DB code: M00155
| RLCP classification | 1.13.30000.10 : Hydrolysis | |
|---|---|---|
| CATH domain | 2.10.70.10 : Complement Module; domain 1 | |
| 2.10.25.10 : Laminin | ||
| 2.40.20.10 : Plasminogen Kringle 4 | ||
| 2.40.10.10 : Thrombin, subunit H | Catalytic domain | |
| 2.40.10.10 : Thrombin, subunit H | Catalytic domain | |
| E.C. | 3.4.21.68 | |
| CSA | 1rtf | |
| M-CSA | 1rtf | |
| MACiE | ||
| CATH domain | Related DB codes (homologues) |
|---|---|
| 2.10.25.10 : Laminin | M00139 M00133 M00212 M00152 M00315 M00316 |
| 2.10.70.10 : Complement Module; domain 1 | M00139 M00315 M00316 |
| 2.40.10.10 : Thrombin, subunit H | M00139 D00214 M00167 D00426 M00133 D00428 D00429 D00430 D00431 D00432 D00433 D00434 D00435 M00227 M00209 D00194 D00197 D00211 D00212 D00216 M00212 D00224 D00497 M00217 M00216 D00528 D00848 D00850 D00851 D00852 D00855 M00152 M00157 M00181 M00315 M00316 M00317 M00348 M00349 T00074 T00410 T00411 |
| 2.40.20.10 : Plasminogen Kringle 4 | M00152 M00157 |
| Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | Contains | RefSeq | MEROPS | Pfam |
|---|---|---|---|---|---|---|
| P00750 |
Tissue-type plasminogen activator (t-plasminogen activator) (t-PA) (tPA) (EC 3.4.21.68)AltName: INN=Alteplase;AltName: INN=Reteplase;
|
None |
Tissue-type plasminogen activator chain A
Tissue-type plasminogen activator chain B |
NP_000921.1
(Protein)
NM_000930.3 (DNA/RNA sequence) NP_127509.1 (Protein) NM_033011.2 (DNA/RNA sequence) |
S01.232
(Serine)
|
PF00008
(EGF)
PF00039 (fn1) PF00051 (Kringle) PF00089 (Trypsin) [Graphical View] |
| P98119 |
Salivary plasminogen activator alpha 1
|
EC
3.4.21.68
DSPA alpha-1 |
None |
S01.239
(Serine)
|
PF00008
(EGF)
PF00039 (fn1) PF00051 (Kringle) PF00089 (Trypsin) [Graphical View] |
| KEGG enzyme name |
|---|
|
t-plasminogen activator
tissue plasminogen activator plasminogen activator, tissue-type tissue-type plasminogen activator tPA t-PA |
| UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
|---|---|---|---|---|---|
| P00750 | TPA_HUMAN | Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin. | Heterodimer of chain A and chain B held by a disulfide bond. Binds to fibrin with high affinity. This interaction leads to an increase in the catalytic efficiency of the enzyme between 100-fold and 1000-fold, due to an increase in affinity for plasminogen. Similarly, binding to heparin increases the activation of plasminogen. Binds to annexin A2, cytokeratin-8, fibronectin and laminin. Binds to mannose receptor and the low- density lipoprotein receptor-related protein (LRP1), these proteins are involved in TPA clearance. Yet unidentified interactions on endothelial cells and vascular smooth muscle cells (VSMC) lead to a 100-fold stimulation of plasminogen activation. In addition, binding to VSMC reduces TPA inhibition by PAI-1 by 30-fold. Binds LRP1B, binding is followed by internalization and degradation. | Secreted, extracellular space. | |
| P98119 | URT1_DESRO | Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin. | Monomer. | Secreted. |
| KEGG Pathways | Map code | Pathways | E.C. |
|---|
| Compound table | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Substrates | Products | Intermediates | ||||||||
| KEGG-id | C00520 | C00471 | I00087 | I00085 | I00086 | |||||
| E.C. | ||||||||||
| Compound | Plasminogen | Plasmin | Peptidyl-tetrahedral intermediate | Acyl-enzyme | Tetrahedral intermediate | |||||
| Type | peptide/protein | peptide/protein | ||||||||
| ChEBI | ||||||||||
| PubChem | ||||||||||
| 1tpgA01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound |
| 1tpgA02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound |
| 1tpmA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound |
| 1tpnA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound |
| 1pk2A |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound |
| 1pmlA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound |
| 1pmlB |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound |
| 1pmlC |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound |
| 1tpkA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound |
| 1tpkB |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound |
| 1tpkC |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound |
| 1a5hA01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound |
| 1a5hB01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound |
| 1a5iA01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:GLU-GLY-ARG-CH2 |
| 1bdaA01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:DSY-GLU-GLY-ARG-CH2 |
| 1bdaB01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:DSY-GLU-GLY-ARG-CH2 |
| 1rtfB01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound |
| 1a5hA02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound |
| 1a5hB02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound |
| 1a5iA02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound |
| 1bdaA02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound |
| 1bdaB02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound |
| 1rtfB02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound |
| Reference for Active-site residues | ||
|---|---|---|
| resource | references | E.C. |
| Swoss-prot; P00750, P98119 & literature [21] | ||
| Active-site residues | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| PDB | Catalytic residues | Cofactor-binding residues | Modified residues | Main-chain involved in catalysis | Comment | |||||
| 1tpgA01 |
|
|
|
|
|
|||||
| 1tpgA02 |
|
|
|
|
|
mutant C83S | ||||
| 1tpmA |
|
|
|
|
|
|||||
| 1tpnA |
|
|
|
|
|
|||||
| 1pk2A |
|
|
|
|
|
|||||
| 1pmlA |
|
|
|
|
|
ASN 26;LYS 33;VAL 34;TYR 35;HIS 64(Chloride binding) | ||||
| 1pmlB |
|
|
|
|
|
ASN 26;LYS 33;VAL 34;TYR 35;HIS 64(Chloride binding) | ||||
| 1pmlC |
|
|
|
|
|
ASN 26;LYS 33;VAL 34;TYR 35;HIS 64(Chloride binding) | ||||
| 1tpkA |
|
|
|
|
|
ASN 26;LYS 33;VAL 34;TYR 35;HIS 64(Chloride binding) | ||||
| 1tpkB |
|
|
|
|
|
ASN 26;LYS 33;VAL 34;TYR 35;HIS 64(Chloride binding) | ||||
| 1tpkC |
|
|
|
|
|
ASN 26;LYS 33;VAL 34;TYR 35;HIS 64(Chloride binding) | ||||
| 1a5hA01 |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 1a5hB01 |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 1a5iA01 |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 1bdaA01 |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 1bdaB01 |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 1rtfB01 |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 1a5hA02 |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 1a5hB02 |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 1a5iA02 |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 1bdaA02 |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 1bdaB02 |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 1rtfB02 |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| References for Catalytic Mechanism | ||
|---|---|---|
| References | Sections | No. of steps in catalysis |
| References | |
|---|---|
| [1] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 6685059 |
| Journal | FEBS Lett |
| Year | 1983 |
| Volume | 163 |
| Pages | 37-41 |
| Authors | Banyai L, Varadi A, Patthy L |
| Title | Common evolutionary origin of the fibrin-binding structures of fibronectin and tissue-type plasminogen activator. |
| Related PDB | |
| Related UniProtKB | |
| [2] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 3030730 |
| Journal | EMBO J |
| Year | 1986 |
| Volume | 5 |
| Pages | 3525-30 |
| Authors | Verheijen JH, Caspers MP, Chang GT, de Munk GA, Pouwels PH, Enger-Valk BE |
| Title | Involvement of finger domain and kringle 2 domain of tissue-type plasminogen activator in fibrin binding and stimulation of activity by fibrin. |
| Related PDB | |
| Related UniProtKB | |
| [3] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 3021732 |
| Journal | J Biol Chem |
| Year | 1986 |
| Volume | 261 |
| Pages | 14214-8 |
| Authors | van Zonneveld AJ, Veerman H, Pannekoek H |
| Title |
On the interaction of the finger and the kringle-2 domain of tissue-type plasminogen activator with fibrin. |
| Related PDB | |
| Related UniProtKB | |
| [4] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 3088564 |
| Journal | Proc Natl Acad Sci U S A |
| Year | 1986 |
| Volume | 83 |
| Pages | 4670-4 |
| Authors | van Zonneveld AJ, Veerman H, Pannekoek H |
| Title | Autonomous functions of structural domains on human tissue-type plasminogen activator. |
| Related PDB | |
| Related UniProtKB | |
| [5] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2846282 |
| Journal | EMBO J |
| Year | 1988 |
| Volume | 7 |
| Pages | 2731-40 |
| Authors | Gething MJ, Adler B, Boose JA, Gerard RD, Madison EL, McGookey D, Meidell RS, Roman LM, Sambrook J |
| Title | Variants of human tissue-type plasminogen activator that lack specific structural domains of the heavy chain. |
| Related PDB | |
| Related UniProtKB | |
| [6] | |
| Resource | |
| Comments | STRUCTURE BY NMR OF KRINGLE 2. |
| Medline ID | 90122799 |
| PubMed ID | 2558718 |
| Journal | Biochemistry |
| Year | 1989 |
| Volume | 28 |
| Pages | 9350-60 |
| Authors | Byeon IJ, Kelley RF, Llinas M |
| Title | 1H NMR structural characterization of a recombinant kringle 2 domain from human tissue-type plasminogen activator. |
| Related PDB | |
| Related UniProtKB | P00750 |
| [7] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2497771 |
| Journal | Biochemistry |
| Year | 1989 |
| Volume | 28 |
| Pages | 1884-91 |
| Authors | Cleary S, Mulkerrin MG, Kelley RF |
| Title | Purification and characterization of tissue plasminogen activator kringle-2 domain expressed in Escherichia coli. |
| Related PDB | |
| Related UniProtKB | |
| [8] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2502177 |
| Journal | Biochemistry |
| Year | 1989 |
| Volume | 28 |
| Pages | 4047-54 |
| Authors | Kelley RF, Cleary S |
| Title | Effect of residue 65 substitutions on thermal stability of tissue plasminogen activator kringle-2 domain. |
| Related PDB | |
| Related UniProtKB | |
| [9] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2477307 |
| Journal | Gene |
| Year | 1989 |
| Volume | 79 |
| Pages | 333-44 |
| Authors | Stern A, Mattes R, Buckel P, Weidle UH |
| Title | Functional topology of human tissue-type plasminogen activator: characterization of two deletion derivatives and of a duplication derivative. |
| Related PDB | |
| Related UniProtKB | |
| [10] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2556697 |
| Journal | Protein Eng |
| Year | 1989 |
| Volume | 3 |
| Pages | 111-6 |
| Authors | Markland W, Pollock D, Livingston DJ |
| Title | Tissue-type plasminogen activator variants with domain duplications and rearrangements. |
| Related PDB | |
| Related UniProtKB | |
| [11] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2108143 |
| Journal | J Biol Chem |
| Year | 1990 |
| Volume | 265 |
| Pages | 5540-5 |
| Authors | Ahern TJ, Morris GE, Barone KM, Horgan PG, Timony GA, Angus LB, Henson KS, Stoudemire JB, Langer-Safer PR, Larsen GR |
| Title |
Site-directed mutagenesis in human tissue-plasminogen activator. |
| Related PDB | |
| Related UniProtKB | |
| [12] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2108167 |
| Journal | J Biol Chem |
| Year | 1990 |
| Volume | 265 |
| Pages | 5170-7 |
| Authors | Burck PJ, Berg DH, Warrick MW, Berg DT, Walls JD, Jaskunas SR, Crisel RM, Weigel B, Vlahos CJ, McClure DB, et al |
| Title | Characterization of a modified human tissue plasminogen activator comprising a kringle-2 and a protease domain. |
| Related PDB | |
| Related UniProtKB | |
| [13] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2115513 |
| Journal | J Biol Chem |
| Year | 1990 |
| Volume | 265 |
| Pages | 12184-91 |
| Authors | Collen D, Lijnen HR, Bulens F, Vandamme AM, Tulinsky A, Nelles L |
| Title | Biochemical and functional characterization of human tissue-type plasminogen activator variants with mutagenized kringle domains. |
| Related PDB | |
| Related UniProtKB | |
| [14] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2117612 |
| Journal | J Biol Chem |
| Year | 1990 |
| Volume | 265 |
| Pages | 14606-11 |
| Authors | Wilhelm OG, Jaskunas SR, Vlahos CJ, Bang NU |
| Title | Functional properties of the recombinant kringle-2 domain of tissue plasminogen activator produced in Escherichia coli. |
| Related PDB | |
| Related UniProtKB | |
| [15] | |
| Resource | |
| Comments | STRUCTURE BY NMR OF KRINGLE 2. |
| Medline ID | 91200042 |
| PubMed ID | 1901789 |
| Journal | Eur J Biochem |
| Year | 1991 |
| Volume | 197 |
| Pages | 155-65 |
| Authors | Byeon IJ, Kelley RF, Llinas M |
| Title |
Kringle-2 domain of the tissue-type plasminogen activator. |
| Related PDB | |
| Related UniProtKB | P00750 |
| [16] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1900516 |
| Journal | J Biol Chem |
| Year | 1991 |
| Volume | 266 |
| Pages | 5191-201 |
| Authors | Bennett WF, Paoni NF, Keyt BA, Botstein D, Jones AJ, Presta L, Wurm FM, Zoller MJ |
| Title | High resolution analysis of functional determinants on human tissue-type plasminogen activator. |
| Related PDB | |
| Related UniProtKB | |
| [17] | |
| Resource | |
| Comments | DISULFIDE BONDS IN KRINGLE 2. |
| Medline ID | 91244765 |
| PubMed ID | 1645336 |
| Journal | J Biol Chem |
| Year | 1991 |
| Volume | 266 |
| Pages | 10070-2 |
| Authors | Vlahos CJ, Wilhelm OG, Hassell T, Jaskunas SR, Bang NU |
| Title | Disulfide pairing of the recombinant kringle-2 domain of tissue plasminogen activator produced in Escherichia coli. |
| Related PDB | |
| Related UniProtKB | P00750 |
| [18] | |
| Resource | |
| Comments | STRUCTURE BY NMR OF KRINGLE 2. |
| Medline ID | 92106329 |
| PubMed ID | 1762144 |
| Journal | J Mol Biol |
| Year | 1991 |
| Volume | 222 |
| Pages | 1035-51 |
| Authors | Byeon IJ, Llinas M |
| Title | Solution structure of the tissue-type plasminogen activator kringle 2 domain complexed to 6-aminohexanoic acid an antifibrinolytic drug. |
| Related PDB | 1pk2 |
| Related UniProtKB | P00750 |
| [19] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1309292 |
| Journal | Arch Biochem Biophys |
| Year | 1992 |
| Volume | 292 |
| Pages | 206-12 |
| Authors | De Serrano VS, Sehl LC, Castellino FJ |
| Title | Direct identification of lysine-33 as the principal cationic center of the omega-amino acid binding site of the recombinant kringle 2 domain of tissue-type plasminogen activator. |
| Related PDB | |
| Related UniProtKB | |
| [20] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1554717 |
| Journal | Biochemistry |
| Year | 1992 |
| Volume | 31 |
| Pages | 3326-35 |
| Authors | De Serrano VS, Castellino FJ |
| Title | Role of tryptophan-74 of the recombinant kringle 2 domain of tissue-type plasminogen activator in its omega-amino acid binding properties. |
| Related PDB | |
| Related UniProtKB | |
| [21] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF KRINGLE 2. |
| Medline ID | 92118803 |
| PubMed ID | 1310033 |
| Journal | Biochemistry |
| Year | 1992 |
| Volume | 31 |
| Pages | 270-9 |
| Authors | de Vos AM, Ultsch MH, Kelley RF, Padmanabhan K, Tulinsky A, Westbrook ML, Kossiakoff AA |
| Title | Crystal structure of the kringle 2 domain of tissue plasminogen activator at 2.4-A resolution. |
| Related PDB | 1tpk |
| Related UniProtKB | P00750 |
| [22] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1731899 |
| Journal | Biochemistry |
| Year | 1992 |
| Volume | 31 |
| Pages | 419-22 |
| Authors | Eastman D, Wurm FM, van Reis R, Higgins DL |
| Title | A region of tissue plasminogen activator that affects plasminogen activation differentially with various fibrin(ogen)-related stimulators. |
| Related PDB | |
| Related UniProtKB | |
| [23] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1315765 |
| Journal | J Biol Chem |
| Year | 1992 |
| Volume | 267 |
| Pages | 9668-77 |
| Authors | Bassel-Duby R, Jiang NY, Bittick T, Madison E, McGookey D, Orth K, Shohet R, Sambrook J, Gething MJ |
| Title | Tyrosine 67 in the epidermal growth factor-like domain of tissue-type plasminogen activator is important for clearance by a specific hepatic receptor. |
| Related PDB | |
| Related UniProtKB | |
| [24] | |
| Resource | |
| Comments | STRUCTURE BY NMR OF 38-85. |
| Medline ID | 92292163 |
| PubMed ID | 1602484 |
| Journal | J Mol Biol |
| Year | 1992 |
| Volume | 225 |
| Pages | 821-33 |
| Authors | Downing AK, Driscoll PC, Harvey TS, Dudgeon TJ, Smith BO, Baron M, Campbell ID |
| Title | Solution structure of the fibrin binding finger domain of tissue-type plasminogen activator determined by 1H nuclear magnetic resonance. |
| Related PDB | 1tpm 1tpn |
| Related UniProtKB | P00750 |
| [25] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1321420 |
| Journal | Protein Eng |
| Year | 1992 |
| Volume | 5 |
| Pages | 93-100 |
| Authors | Kohnert U, Rudolph R, Verheijen JH, Weening-Verhoeff EJ, Stern A, Opitz U, Martin U, Lill H, Prinz H, Lechner M, et al |
| Title | Biochemical properties of the kringle 2 and protease domains are maintained in the refolded t-PA deletion variant BM 06.022. |
| Related PDB | |
| Related UniProtKB | |
| [26] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1287881 |
| Journal | Thromb Haemost |
| Year | 1992 |
| Volume | 68 |
| Pages | 672-7 |
| Authors | Yahara H, Matsumoto K, Maruyama H, Nagaoka T, Ikenaka Y, Yajima K, Fukao H, Ueshima S, Matsuo O |
| Title | Recombinant variants of tissue-type plasminogen activator containing amino acid substitutions in the finger domain. |
| Related PDB | |
| Related UniProtKB | |
| [27] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8424682 |
| Journal | Arch Biochem Biophys |
| Year | 1993 |
| Volume | 300 |
| Pages | 472-82 |
| Authors | Rydzewski A, Castellino FJ |
| Title | The effector roles of kringle 1 and kringle 2 in the enzymatic properties of recombinant tissue-type plasminogen activator as revealed by generation of recombinant molecules containing each kringle linked to the protease domain. |
| Related PDB | |
| Related UniProtKB | |
| [28] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8385482 |
| Journal | Biochemistry |
| Year | 1993 |
| Volume | 32 |
| Pages | 3540-8 |
| Authors | De Serrano VS, Castellino FJ |
| Title | Specific anionic residues of the recombinant kringle 2 domain of tissue-type plasminogen activator that are responsible for stabilization of its interaction with omega-amino acid ligands. |
| Related PDB | |
| Related UniProtKB | |
| [29] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8449917 |
| Journal | J Biol Chem |
| Year | 1993 |
| Volume | 268 |
| Pages | 5550-6 |
| Authors | Lubin IM, Caban R, Runge MS |
| Title | The tissue plasminogen activator finger domain confers fibrin-dependent enhancement of catalytic activity to single-chain urokinase-type plasminogen activator. |
| Related PDB | |
| Related UniProtKB | |
| [30] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8030371 |
| Journal | Acta Biochim Pol |
| Year | 1994 |
| Volume | 41 |
| Pages | 25-34 |
| Authors | Pietrucha T, Stec WJ, Okruszek A, Uznanski B, Koziolkiewicz M, Wilk A, Plucienniczak A, Swiatkowska M, Cierniewski CS |
| Title |
The epidermal growth factor-like domain from tissue plasminogen activator. |
| Related PDB | |
| Related UniProtKB | |
| [31] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8142348 |
| Journal | Biochemistry |
| Year | 1994 |
| Volume | 33 |
| Pages | 3509-14 |
| Authors | De Serrano VS, Castellino FJ |
| Title | Involvement of tyrosine-76 of the kringle 2 domain of tissue-type plasminogen activator in its thermal stability and its omega-amino acid ligand binding site. |
| Related PDB | |
| Related UniProtKB | |
| [32] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8312251 |
| Journal | Biochemistry |
| Year | 1994 |
| Volume | 33 |
| Pages | 1340-4 |
| Authors | De Serrano VS, Castellino FJ |
| Title | Role of the strictly conserved tryptophan-25 residue in the stabilization of the structure and in the ligand binding properties of the kringle 2 domain of tissue-type plasminogen activator. |
| Related PDB | |
| Related UniProtKB | |
| [33] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8175674 |
| Journal | J Biol Chem |
| Year | 1994 |
| Volume | 269 |
| Pages | 12639-44 |
| Authors | Horrevoets AJ, Smilde A, de Vries C, Pannekoek H |
| Title | The specific roles of finger and kringle 2 domains of tissue-type plasminogen activator during in vitro fibrinolysis. |
| Related PDB | |
| Related UniProtKB | |
| [34] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 8069221 |
| Journal | Protein Sci |
| Year | 1994 |
| Volume | 3 |
| Pages | 898-910 |
| Authors | Padmanabhan K, Wu TP, Ravichandran KG, Tulinsky A |
| Title | Kringle-kringle interactions in multimer kringle structures. |
| Related PDB | 1pmk 1pml |
| Related UniProtKB | |
| [35] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7740461 |
| Journal | Thromb Haemost |
| Year | 1994 |
| Volume | 72 |
| Pages | 900-5 |
| Authors | Stringer HA, van Swieten P, Horrevoets AJ, Smilde A, Pannekoek H |
| Title | The role of the finger domain of tissue-type plasminogen activator (t-PA) and plasminogen activator inhibitor 1 (PAI-1) in initiation and progression of thrombolysis. |
| Related PDB | |
| Related UniProtKB | |
| [36] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7893685 |
| Journal | Biochemistry |
| Year | 1995 |
| Volume | 34 |
| Pages | 2739-50 |
| Authors | Byeon IJ, Kelley RF, Mulkerrin MG, An SS, Llinas M |
| Title | Ligand binding to the tissue-type plasminogen activator kringle 2 domain: structural characterization by 1H-NMR. |
| Related PDB | |
| Related UniProtKB | |
| [37] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7711052 |
| Journal | Biochim Biophys Acta |
| Year | 1995 |
| Volume | 1248 |
| Pages | 1-10 |
| Authors | Rudd PM, Woods RJ, Wormald MR, Opdenakker G, Downing AK, Campbell ID, Dwek RA |
| Title |
The effects of variable glycosylation on the functional activities of ribonuclease, |
| Related PDB | |
| Related UniProtKB | |
| [38] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8530479 |
| Journal | J Biol Chem |
| Year | 1995 |
| Volume | 270 |
| Pages | 30486-90 |
| Authors | Smith JW, Tachias K, Madison EL |
| Title | Protein loop grafting to construct a variant of tissue-type plasminogen activator that binds platelet integrin alpha IIb beta 3. |
| Related PDB | |
| Related UniProtKB | |
| [39] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7479691 |
| Journal | Protein Eng |
| Year | 1995 |
| Volume | 8 |
| Pages | 293-300 |
| Authors | Bakker AH, Rehberg EF, Marotti KR, Verheijen JH |
| Title | The position of the structurally autonomous kringle 2 domain influences the functional features of tissue-type plasminogen activator. |
| Related PDB | |
| Related UniProtKB | |
| [40] | |
| Resource | |
| Comments | STRUCTURE BY NMR OF 36-126. |
| Medline ID | 96027104 |
| PubMed ID | 7582899 |
| Journal | Structure |
| Year | 1995 |
| Volume | 3 |
| Pages | 823-33 |
| Authors | Smith BO, Downing AK, Driscoll PC, Dudgeon TJ, Campbell ID |
| Title | The solution structure and backbone dynamics of the fibronectin type I and epidermal growth factor-like pair of modules of tissue-type plasminogen activator. |
| Related PDB | 1tpg |
| Related UniProtKB | P00750 |
| [41] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8605163 |
| Journal | Biochemistry |
| Year | 1996 |
| Volume | 35 |
| Pages | 3270-6 |
| Authors | Hu CK, Kohnert U, Sturzebecher J, Fischer S, Llinas M |
| Title | Complexation of the tissue plasminogen activator protease with benzamidine-type inhibitors: interference by the kringle 2 module. |
| Related PDB | |
| Related UniProtKB | |
| [42] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF CATALYTIC DOMAIN. |
| Medline ID | 96200985 |
| PubMed ID | 8613982 |
| Journal | J Mol Biol |
| Year | 1996 |
| Volume | 258 |
| Pages | 117-35 |
| Authors | Lamba D, Bauer M, Huber R, Fischer S, Rudolph R, Kohnert U, Bode W |
| Title | The 2.3 A crystal structure of the catalytic domain of recombinant two-chain human tissue-type plasminogen activator. |
| Related PDB | 1rtf |
| Related UniProtKB | P00750 |
| [43] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8736495 |
| Journal | Protein Eng |
| Year | 1996 |
| Volume | 9 |
| Pages | 283-90 |
| Authors | Sehl LC, Nguyen HV, Berleau LT, Arcila P, Bennett WF, Keyt BA |
| Title | Locating the unpaired cysteine of tissue-type plasminogen activator. |
| Related PDB | |
| Related UniProtKB | |
| [44] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9201906 |
| Journal | Biochemistry |
| Year | 1997 |
| Volume | 36 |
| Pages | 7652-63 |
| Authors | Chang Y, Zajicek J, Castellino FJ |
| Title |
Role of tryptophan-63 of the kringle 2 domain of tissue-type plasminogen activator in its thermal stability, |
| Related PDB | |
| Related UniProtKB | |
| [45] | |
| Resource | |
| Comments | TISSUE=Salivary gland; |
| Medline ID | 98022741 |
| PubMed ID | 9354616 |
| Journal | Biochemistry |
| Year | 1997 |
| Volume | 36 |
| Pages | 13483-93 |
| Authors | Renatus M, Stubbs MT, Huber R, Bringmann P, Donner P, Schleuning WD, Bode W |
| Title | Catalytic domain structure of vampire bat plasminogen activator: a molecular paradigm for proteolysis without activation cleavage. |
| Related PDB | 1a5i |
| Related UniProtKB | P98119 |
| [46] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9434908 |
| Journal | Curr Opin Struct Biol |
| Year | 1997 |
| Volume | 7 |
| Pages | 865-72 |
| Authors | Bode W, Renatus M |
| Title | Tissue-type plasminogen activator: variants and crystal/solution structures demarcate structural determinants of function. |
| Related PDB | |
| Related UniProtKB | |
| [47] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) OF CATALYTIC DOMAIN. |
| Medline ID | 97449126 |
| PubMed ID | 9305622 |
| Journal | EMBO J |
| Year | 1997 |
| Volume | 16 |
| Pages | 4797-805 |
| Authors | Renatus M, Engh RA, Stubbs MT, Huber R, Fischer S, Kohnert U, Bode W |
| Title | Lysine 156 promotes the anomalous proenzyme activity of tPA: X-ray crystal structure of single-chain human tPA. |
| Related PDB | 1bda |
| Related UniProtKB | P00750 |
| [48] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 9268299 |
| Journal | J Biol Chem |
| Year | 1997 |
| Volume | 272 |
| Pages | 21713-9 |
| Authors | Renatus M, Bode W, Huber R, Sturzebecher J, Prasa D, Fischer S, Kohnert U, Stubbs MT |
| Title |
Structural mapping of the active site specificity determinants of human tissue-type plasminogen activator. |
| Related PDB | 1a5h |
| Related UniProtKB | |
| [49] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10543954 |
| Journal | J Mol Biol |
| Year | 1999 |
| Volume | 293 |
| Pages | 613-27 |
| Authors | Dekker RJ, Eichinger A, Stoop AA, Bode W, Pannekoek H, Horrevoets AJ |
| Title | The variable region-1 from tissue-type plasminogen activator confers specificity for plasminogen activator inhibitor-1 to thrombin by facilitating catalysis: release of a kinetic block by a heterologous protein surface loop. |
| Related PDB | |
| Related UniProtKB | |
| [50] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10408340 |
| Journal | J Pept Res |
| Year | 1999 |
| Volume | 53 |
| Pages | 656-64 |
| Authors | Chang Y, Nilsen SL, Castellino FJ |
| Title | Functional and structural consequences of aromatic residue substitutions within the kringle-2 domain of tissue-type plasminogen activator. |
| Related PDB | |
| Related UniProtKB | |
| [51] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10744692 |
| Journal | J Biol Chem |
| Year | 2000 |
| Volume | 275 |
| Pages | 10112-20 |
| Authors | Stewart RJ, Fredenburgh JC, Leslie BA, Keyt BA, Rischke JA, Weitz JI |
| Title | Identification of the mechanism responsible for the increased fibrin specificity of TNK-tissue plasminogen activator relative to tissue plasminogen activator. |
| Related PDB | |
| Related UniProtKB | |
| [52] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12696753 |
| Journal | Curr Top Dev Biol |
| Year | 2003 |
| Volume | 54 |
| Pages | 263-312 |
| Authors | Ellis V |
| Title | Plasminogen activation at the cell surface. |
| Related PDB | |
| Related UniProtKB | |
| [53] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 14500731 |
| Journal | J Biol Chem |
| Year | 2003 |
| Volume | 278 |
| Pages | 48197-203 |
| Authors | Perron MJ, Blouse GE, Shore JD |
| Title | Distortion of the catalytic domain of tissue-type plasminogen activator by plasminogen activator inhibitor-1 coincides with the formation of stable serpin-proteinase complexes. |
| Related PDB | |
| Related UniProtKB | |
| Comments |
|---|
|
This enzyme belongs to the peptidase family-S1.
This enzyme is composed of six domains; fibronectin type-I domain, The PDB structures correspond to the following domains. (PDB;1tpg, PDB;1pk2, PDB;1a5hA, As this enzyme has got a classical catalytic triad composed of Ser/His/Asp, |
| Created | Updated |
|---|---|
| 2004-08-13 | 2011-02-21 |