DB code: D00434

RLCP classification	1.13.30000.10 : Hydrolysis
CATH domain	2.40.10.10 : Thrombin, subunit H	Catalytic domain
	2.40.10.10 : Thrombin, subunit H	Catalytic domain
E.C.	3.4.21.80
CSA
M-CSA
MACiE

CATH domain	Related DB codes (homologues)
2.40.10.10 : Thrombin, subunit H	M00139 D00214 M00167 D00426 M00133 D00428 D00429 D00430 D00431 D00432 D00433 D00435 M00227 M00209 D00194 D00197 D00211 D00212 D00216 M00212 D00224 D00497 M00217 M00216 D00528 D00848 D00850 D00851 D00852 D00855 M00152 M00155 M00157 M00181 M00315 M00316 M00317 M00348 M00349 T00074 T00410 T00411

Uniprot Enzyme Name
UniprotKB	Protein name	Synonyms	MEROPS	Pfam
P00776	Streptogrisin-A	EC 3.4.21.80 Serine protease A SGPA Pronase enzyme A	S01.261 (Serine)	PF02983 (Pro_Al_protease) PF00089 (Trypsin) [Graphical View]

KEGG enzyme name
streptogrisin A Streptomyces griseus protease A protease A proteinase A Streptomyces griseus proteinase A Streptomyces griseus serine proteinase 3 Streptomyces griseus serine proteinase A

UniprotKB: Accession Number	Entry name	Activity	Subunit	Subcellular location	Cofactor
P00776	PRTA_STRGR	Hydrolysis of proteins with specificity similar to chymotrypsin.	Monomer.

KEGG Pathways
Map code	Pathways	E.C.

Compound table
						Substrates			Products		Intermediates
KEGG-id						C00012	C00017	C00001	C00012	C00017	I00087	I00085	I00086
E.C.
Compound						Peptide	Protein	H2O	Peptide	Protein	Peptidyl-tetrahedral intermediate	Acyl-enzyme	Tetrahedral intermediate
Type						peptide/protein	peptide/protein	H2O	peptide/protein	peptide/protein
ChEBI								15377 15377
PubChem								22247451 962 22247451 962
1sgcA01							Unbound	Unbound	Unbound	Unbound	Unbound	Unbound	Unbound
2sgaA01							Unbound	Unbound	Unbound	Unbound	Unbound	Unbound	Unbound
3sgaE01							Unbound	Unbound	Unbound	Unbound	Unbound	Unbound	Unbound
4sgaE01							Unbound	Unbound	Unbound	Unbound	Unbound	Unbound	Unbound
5sgaE01							Unbound	Unbound	Unbound	Unbound	Unbound	Unbound	Unbound
1sgcA02							Unbound	Unbound	Unbound	Unbound	Unbound	Unbound	Transition-state-analogue:CST
2sgaA02							Unbound	Unbound	Unbound	Unbound	Unbound	Unbound	Unbound
3sgaE02							Unbound	Unbound	Unbound	Unbound	Unbound	Intermediate-bound:PHA-PRO-ALA-PRO-ACE (chain P)	Unbound
4sgaE02							Unbound	Unbound	Bound:PHE-PRO-ALA-PRO-ACE (chain P)	Unbound	Unbound	Unbound	Unbound
5sgaE02							Unbound	Unbound	Bound:TYR-PRO-ALA-PRO-ACE (chain P)	Unbound	Unbound	Unbound	Unbound

Reference for Active-site residues
resource	references	E.C.
Swiss-prot;P00776 & literature;[6]

Active-site residues
PDB						Catalytic residues	Cofactor-binding residues	Modified residues	Main-chain involved in catalysis	Comment
1sgcA01						HIS 57;ASP 102
2sgaA01						HIS 57;ASP 102
3sgaE01						HIS 57;ASP 102
4sgaE01						HIS 57;ASP 102
5sgaE01						HIS 57;ASP 102
1sgcA02						SER 195			GLY 193;SER 195
2sgaA02						SER 195			GLY 193;SER 195
3sgaE02						SER 195			GLY 193;SER 195
4sgaE02						SER 195			GLY 193;SER 195
5sgaE02						SER 195			GLY 193;SER 195

References for Catalytic Mechanism
References	Sections	No. of steps in catalysis
[3]	Fig.1, p.78-85	6
[4]	p.102-103

References
[1]
Resource
Comments	X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
Medline ID	79050558
PubMed ID	101674
Journal	J Mol Biol
Year	1978
Volume	124
Pages	261-83
Authors	Brayer GD, Delbaere LT, James MN
Title	Molecular structure of crystalline Streptomyces griseus protease A at 2.8 A resolution. II. Molecular conformation, comparison with alpha-chymotrypsin and active-site geometry.
Related PDB
Related UniProtKB	P00776
[2]
Resource
Comments	X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
Medline ID	80140489
PubMed ID	119870
Journal	J Mol Biol
Year	1979
Volume	134
Pages	781-804
Authors	Sielecki AR, Hendrickson WA, Broughton CG, Delbaere LT, Brayer GD, James MN
Title	Protein structure refinement: Streptomyces griseus serine protease A at 1.8 A resolution.
Related PDB
Related UniProtKB	P00776
[3]
Resource
Comments	X-ray crystallography
Medline ID
PubMed ID	6783761
Journal	J Mol Biol
Year	1980
Volume	144
Pages	43-88
Authors	James MN, Sielecki AR, Brayer GD, Delbaere LT, Bauer CA
Title	Structures of product and inhibitor complexes of Streptomyces griseus protease A at 1.8 A resolution. A model for serine protease catalysis.
Related PDB	3sga 4sga 5sga
Related UniProtKB
[4]
Resource
Comments	X-ray crystallography
Medline ID
PubMed ID	3892018
Journal	J Mol Biol
Year	1985
Volume	183
Pages	89-103
Authors	Delbaere LT, Brayer GD
Title	The 1.8 A structure of the complex between chymostatin and Streptomyces griseus protease A. A model for serine protease catalytic tetrahedral intermediates.
Related PDB	1sgc
Related UniProtKB
[5]
Resource
Comments
Medline ID
PubMed ID	3900416
Journal	J Mol Biol
Year	1985
Volume	184
Pages	479-502
Authors	Fujinaga M, Delbaere LT, Brayer GD, James MN
Title	Refined structure of alpha-lytic protease at 1.7 A resolution. Analysis of hydrogen bonding and solvent structure.
Related PDB
Related UniProtKB
[6]
Resource
Comments	X-ray crystallography
Medline ID
PubMed ID	3892015
Journal	J Mol Biol
Year	1985
Volume	182
Pages	555-66
Authors	Moult J, Sussman F, James MN
Title	Electron density calculations as an extension of protein structure refinement. Streptomyces griseus protease A at 1.5 A resolution.
Related PDB	2sga
Related UniProtKB
[7]
Resource
Comments
Medline ID
PubMed ID	1744078
Journal	J Biol Chem
Year	1991
Volume	266
Pages	22851-7
Authors	Nebes VL, Jones EW
Title	Activation of the proteinase B precursor of the yeast Saccharomyces cerevisiae by autocatalysis and by an internal sequence.
Related PDB
Related UniProtKB
[8]
Resource
Comments
Medline ID
PubMed ID	8415632
Journal	Proc Natl Acad Sci U S A
Year	1993
Volume	90
Pages	8920-4
Authors	Kitson DH, Avbelj F, Moult J, Nguyen DT, Mertz JE, Hadzi D, Hagler AT
Title	On achieving better than 1-A accuracy in a simulation of a large protein: Streptomyces griseus protease A.
Related PDB
Related UniProtKB
[9]
Resource
Comments
Medline ID
PubMed ID	8119298
Journal	Eur J Biochem
Year	1994
Volume	220
Pages	55-61
Authors	Kitadokoro K, Tsuzuki H, Nakamura E, Sato T, Teraoka H
Title	Purification, characterization, primary structure, crystallization and preliminary crystallographic study of a serine proteinase from Streptomyces fradiae ATCC 14544.
Related PDB
Related UniProtKB
[10]
Resource
Comments
Medline ID
PubMed ID	7925392
Journal	Eur J Biochem
Year	1994
Volume	224
Pages	735-42
Authors	Kitadokoro K, Tsuzuki H, Okamoto H, Sato T
Title	Crystal structure analysis of a serine proteinase from Streptomyces fradiae at 0.16-nm resolution and molecular modeling of an acidic-amino-acid-specific proteinase.
Related PDB
Related UniProtKB
[11]
Resource
Comments
Medline ID
PubMed ID	8057380
Journal	J Mol Biol
Year	1994
Volume	241
Pages	574-87
Authors	Blanchard H, James MN
Title	A crystallographic re-investigation into the structure of Streptomyces griseus proteinase A reveals an acyl-enzyme intermediate.
Related PDB
Related UniProtKB

Comments
This enzyme belongs to the peptidase family-S2A. According to the literature [3] & [4], the enzyme has got a similar catalytic triad (Ser/His/Asp) to that of trypsin, suggesting a similar mechanism.

Created	Updated
2004-01-17	2011-02-21