DB code: M00157
| RLCP classification | 1.13.30120.22 : Hydrolysis | |
|---|---|---|
| CATH domain | -.-.-.- : | |
| 2.40.20.10 : Plasminogen Kringle 4 | ||
| 2.40.20.10 : Plasminogen Kringle 4 | ||
| 2.40.20.10 : Plasminogen Kringle 4 | ||
| 2.40.20.10 : Plasminogen Kringle 4 | ||
| 2.40.20.10 : Plasminogen Kringle 4 | ||
| 2.40.10.10 : Thrombin, subunit H | Catalytic domain | |
| 2.40.10.10 : Thrombin, subunit H | Catalytic domain | |
| E.C. | 3.4.21.7 | |
| CSA | 1ddj 1qrz | |
| M-CSA | 1ddj 1qrz | |
| MACiE | ||
| CATH domain | Related DB codes (homologues) |
|---|---|
| 2.40.10.10 : Thrombin, subunit H | M00139 D00214 M00167 D00426 M00133 D00428 D00429 D00430 D00431 D00432 D00433 D00434 D00435 M00227 M00209 D00194 D00197 D00211 D00212 D00216 M00212 D00224 D00497 M00217 M00216 D00528 D00848 D00850 D00851 D00852 D00855 M00152 M00155 M00181 M00315 M00316 M00317 M00348 M00349 T00074 T00410 T00411 |
| 2.40.20.10 : Plasminogen Kringle 4 | M00152 M00155 |
| Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | Contains | RefSeq | MEROPS | Pfam |
|---|---|---|---|---|---|---|
| P00747 |
Plasminogen
|
EC
3.4.21.7
|
Plasmin heavy chain A
Activation peptide Angiostatin Plasmin heavy chain A, short form Plasmin light chain B |
NP_000292.1
(Protein)
NM_000301.3 (DNA/RNA sequence) |
S01.233
(Serine)
|
PF00051
(Kringle)
PF00024 (PAN_1) PF00089 (Trypsin) [Graphical View] |
| KEGG enzyme name |
|---|
|
plasmin
fibrinase fibrinolysin actase serum tryptase thrombolysin |
| UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
|---|---|---|---|---|---|
| P00747 | PLMN_HUMAN | Preferential cleavage: Lys-|-Xaa > Arg-|-Xaa, higher selectivity than trypsin. Converts fibrin into soluble products. | Interacts with AMOT and CSPG4 (also true for angiostatin). | Secreted. |
| KEGG Pathways | Map code | Pathways | E.C. |
|---|
| Compound table | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Substrates | Products | Intermediates | |||||||||||
| KEGG-id | C00001 | C00290 | C02873 | C00069 | C00045 | I00087 | I00085 | I00086 | |||||
| E.C. | |||||||||||||
| Compound | H2O | Fibrin | Peptide(Lys-, Arg-) | Alcohol | Amino acid | Peptidyl-tetrahedral intermediate | Acyl-enzyme | Tetrahedral intermediate | |||||
| Type | H2O | amine group,peptide/protein | amino acids,amine group,peptide/protein | carbohydrate | amino acids | ||||||||
| ChEBI |
15377 15377 |
5054 5054 |
|||||||||||
| PubChem |
22247451 962 22247451 962 |
439199 439199 |
|||||||||||
| 1ki0A01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ceaA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ceaB |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1cebA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1cebB |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1hpjA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1hpkA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1pkrA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ki0A02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1b2iA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1i5kA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1i5kB |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ki0A03 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1krnA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1pk4A |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1pmkA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1pmkB |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2pk4A |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 5hpgA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 5hpgB |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1bmlA01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1bmlB01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1buiA01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:GLU-GLY-MAI (chain I) | |
| 1buiB01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ddjA01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ddjB01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ddjC01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ddjD01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1l4dA01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1l4zA01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1qrzA01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1qrzB01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1qrzC01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1qrzD01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1rjxB01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1bmlA02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1bmlB02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1buiA02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1buiB02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ddjA02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ddjB02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ddjC02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ddjD02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1l4dA02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1l4zA02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1qrzA02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1qrzB02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1qrzC02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1qrzD02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1rjxB02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| Reference for Active-site residues | ||
|---|---|---|
| resource | references | E.C. |
| PDB;1bml, 1bui, 1qrz & Swiss-prot;P00747 | ||
| Active-site residues | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| PDB | Catalytic residues | Cofactor-binding residues | Modified residues | Main-chain involved in catalysis | Comment | |||||
| 1ki0A01 |
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|||||
| 1ceaA |
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| 1ceaB |
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|||||
| 1cebA |
|
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| 1cebB |
|
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|||||
| 1hpjA |
|
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| 1hpkA |
|
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| 1pkrA |
|
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|||||
| 1ki0A02 |
|
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| 1b2iA |
|
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|
mutant C181T, E182S, C188G | ||||
| 1i5kA |
|
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|
|
mutant C4G, E56D, L72Y | ||||
| 1i5kB |
|
|
|
|
|
mutant C4G, E56D, L72Y | ||||
| 1ki0A03 |
|
|
|
|
|
mutant N289E | ||||
| 1krnA |
|
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|||||
| 1pk4A |
|
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|||||
| 1pmkA |
|
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|
|||||
| 1pmkB |
|
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| 2pk4A |
|
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| 5hpgA |
|
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|||||
| 5hpgB |
|
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| 1bmlA01 |
|
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|
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|
mutant S741A | ||||
| 1bmlB01 |
|
|
|
|
|
mutant S741A | ||||
| 1buiA01 |
|
|
|
|
|
SER 195 | ||||
| 1buiB01 |
|
|
|
|
|
SER 195 | ||||
| 1ddjA01 |
|
|
|
|
|
mutant S741A | ||||
| 1ddjB01 |
|
|
|
|
|
mutant S741A | ||||
| 1ddjC01 |
|
|
|
|
|
mutant S741A | ||||
| 1ddjD01 |
|
|
|
|
|
mutant S741A | ||||
| 1l4dA01 |
|
|
|
|
|
mutant S741A | ||||
| 1l4zA01 |
|
|
|
|
|
mutant S741A | ||||
| 1qrzA01 |
|
|
|
|
|
SER 741 | mutant V673M | |||
| 1qrzB01 |
|
|
|
|
|
SER 741 | mutant V673M | |||
| 1qrzC01 |
|
|
|
|
|
SER 741 | mutant V673M | |||
| 1qrzD01 |
|
|
|
|
|
SER 741 | mutant V673M | |||
| 1rjxB01 |
|
|
|
|
|
SER 741 | mutant K698M | |||
| 1bmlA02 |
|
|
|
|
|
HIS 603;ASP 646 | SER 578(Phospholylated) | |||
| 1bmlB02 |
|
|
|
|
|
HIS 603;ASP 646 | SER 578(Phospholylated) | |||
| 1buiA02 |
|
|
|
|
|
HIS 57;ASP 102 | SER 32(Phospholylated) | |||
| 1buiB02 |
|
|
|
|
|
HIS 57;ASP 102 | SER 32(Phospholylated) | |||
| 1ddjA02 |
|
|
|
|
|
HIS 603;ASP 646 | SER 578(Phospholylated) | |||
| 1ddjB02 |
|
|
|
|
|
HIS 603;ASP 646 | SER 578(Phospholylated) | |||
| 1ddjC02 |
|
|
|
|
|
HIS 603;ASP 646 | SER 578(Phospholylated) | |||
| 1ddjD02 |
|
|
|
|
|
HIS 603;ASP 646 | SER 578(Phospholylated) | |||
| 1l4dA02 |
|
|
|
|
|
HIS 603;ASP 646 | SER 578(Phospholylated) | |||
| 1l4zA02 |
|
|
|
|
|
HIS 603;ASP 646 | SER 578(Phospholylated) | |||
| 1qrzA02 |
|
|
|
|
|
HIS 603;ASP 646 | SER 578(Phospholylated) | mutant M585Q, M788L | ||
| 1qrzB02 |
|
|
|
|
|
HIS 603;ASP 646 | SER 578(Phospholylated) | mutant M585Q, M788L | ||
| 1qrzC02 |
|
|
|
|
|
HIS 603;ASP 646 | SER 578(Phospholylated) | mutant M585Q, M788L | ||
| 1qrzD02 |
|
|
|
|
|
HIS 603;ASP 646 | SER 578(Phospholylated) | mutant M585Q, M788L | ||
| 1rjxB02 |
|
|
|
|
|
HIS 603;ASP 646 | SER 578(Phospholylated) | |||
| References for Catalytic Mechanism | ||
|---|---|---|
| References | Sections | No. of steps in catalysis |
|
[49]
|
p.906, p.907-909 | |
| References | |
|---|---|
| [1] | |
| Resource | |
| Comments | ACTIVE SITE. |
| Medline ID | 69234739 |
| PubMed ID | 4240117 |
| Journal | J Biol Chem |
| Year | 1969 |
| Volume | 244 |
| Pages | 3590-7 |
| Authors | Groskopf WR, Summaria L, Robbins KC |
| Title |
Studies on the active center of human plasmin. |
| Related PDB | |
| Related UniProtKB | P00747 |
| [2] | |
| Resource | |
| Comments | ACTIVE SITE. |
| Medline ID | 73149248 |
| PubMed ID | 4694729 |
| Journal | J Biol Chem |
| Year | 1973 |
| Volume | 248 |
| Pages | 1631-3 |
| Authors | Robbins KC, Bernabe P, Arzadon L, Summaria L |
| Title |
The primary structure of human plasminogen. |
| Related PDB | |
| Related UniProtKB | P00747 |
| [3] | |
| Resource | |
| Comments | VARIANT THROMBOPHILIA THR-620. |
| Medline ID | 83065132 |
| PubMed ID | 6216475 |
| Journal | Proc Natl Acad Sci U S A |
| Year | 1982 |
| Volume | 79 |
| Pages | 6132-6 |
| Authors | Miyata T, Iwanaga S, Sakata Y, Aoki N |
| Title | Plasminogen Tochigi: inactive plasmin resulting from replacement of alanine-600 by threonine in the active site. |
| Related PDB | |
| Related UniProtKB | P00747 |
| [4] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 6311534 |
| Journal | Eur J Biochem |
| Year | 1983 |
| Volume | 135 |
| Pages | 379-91 |
| Authors | Llinas M, De Marco A, Hochschwender SM, Laursen RA |
| Title |
A 1H-NMR study of isolated domains from human plasminogen. |
| Related PDB | |
| Related UniProtKB | |
| [5] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2933077 |
| Journal | Biochim Biophys Acta |
| Year | 1985 |
| Volume | 832 |
| Pages | 215-9 |
| Authors | Ascenzi P, Torroni A, Menegatti E, Guarneri M, Amiconi G |
| Title |
Catalytic properties of human Lys77-plasmin. |
| Related PDB | |
| Related UniProtKB | |
| [6] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2982407 |
| Journal | Biochim Biophys Acta |
| Year | 1985 |
| Volume | 827 |
| Pages | 369-80 |
| Authors | De Marco A, Laursen RA, Llinas M |
| Title |
Proton Overhauser experiments on kringle 4 from human plasminogen. |
| Related PDB | |
| Related UniProtKB | |
| [7] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 4041537 |
| Journal | Biophys J |
| Year | 1985 |
| Volume | 48 |
| Pages | 411-22 |
| Authors | De Marco A, Motta A, Llinas M, Laursen RA |
| Title |
Macro- and micro-stabilities of the kringle 4 domain from plasminogen. |
| Related PDB | |
| Related UniProtKB | |
| [8] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 3004350 |
| Journal | Arch Biochem Biophys |
| Year | 1986 |
| Volume | 244 |
| Pages | 727-41 |
| Authors | De Marco A, Laursen RA, Llinas M |
| Title | 1H-NMR spectroscopic manifestations of ligand binding to the kringle 4 domain of human plasminogen. |
| Related PDB | |
| Related UniProtKB | |
| [9] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 3803570 |
| Journal | FEBS Lett |
| Year | 1986 |
| Volume | 209 |
| Pages | 111-6 |
| Authors | Williams RJ, Esnouf P, Lawrence M, Cederholm-Williams SA |
| Title | The similarities and differences in structures between kringle 1 of prothrombin and kringle 4 of plasminogen. |
| Related PDB | |
| Related UniProtKB | |
| [10] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 3531210 |
| Journal | J Biol Chem |
| Year | 1986 |
| Volume | 261 |
| Pages | 13684-92 |
| Authors | Motta A, Laursen RA, Rajan N, Llinas M |
| Title |
Proton magnetic resonance study of kringle 1 from human plasminogen. |
| Related PDB | |
| Related UniProtKB | |
| [11] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2820478 |
| Journal | Biochemistry |
| Year | 1987 |
| Volume | 26 |
| Pages | 3827-36 |
| Authors | Motta A, Laursen RA, Llinas M, Tulinsky A, Park CH |
| Title | Complete assignment of the aromatic proton magnetic resonance spectrum of the kringle 1 domain from human plasminogen: structure of the ligand-binding site. |
| Related PDB | |
| Related UniProtKB | |
| [12] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 3030435 |
| Journal | Biochim Biophys Acta |
| Year | 1987 |
| Volume | 912 |
| Pages | 254-69 |
| Authors | Thewes T, Ramesh V, Simplaceanu EL, Llinas M |
| Title |
Isolation, |
| Related PDB | |
| Related UniProtKB | |
| [13] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2828641 |
| Journal | J Mol Biol |
| Year | 1987 |
| Volume | 198 |
| Pages | 481-98 |
| Authors | Ramesh V, Petros AM, Llinas M, Tulinsky A, Park CH |
| Title |
Proton magnetic resonance study of lysine-binding to the kringle 4 domain of human plasminogen. |
| Related PDB | |
| Related UniProtKB | |
| [14] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2954262 |
| Journal | Thromb Res |
| Year | 1987 |
| Volume | 45 |
| Pages | 451-62 |
| Authors | Hijikata-Okunomiya A, Okamoto S, Kikumoto R, Tamao Y, Ohkubo K, Tezuka T, Tonomura S, Matsumoto O |
| Title |
Similarity and dissimilarity in the stereogeometry of the active sites of thrombin, |
| Related PDB | |
| Related UniProtKB | |
| [15] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 3338450 |
| Journal | Eur J Biochem |
| Year | 1988 |
| Volume | 170 |
| Pages | 539-48 |
| Authors | Mabbutt BC, Williams RJ |
| Title | Two-dimensional 1H-NMR studies of the solution structure of plasminogen kringle 4. |
| Related PDB | |
| Related UniProtKB | |
| [16] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2841130 |
| Journal | Eur J Biochem |
| Year | 1988 |
| Volume | 175 |
| Pages | 237-49 |
| Authors | Thewes T, Ramesh V, Simplaceanu EL, Llinas M |
| Title |
Analysis of the aromatic 1H-NMR spectrum of the kringle 5 domain from human plasminogen. |
| Related PDB | |
| Related UniProtKB | |
| [17] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 3135547 |
| Journal | Proteins |
| Year | 1988 |
| Volume | 3 |
| Pages | 85-96 |
| Authors | Tulinsky A, Park CH, Mao B, Llinas M |
| Title | Lysine/fibrin binding sites of kringles modeled after the structure of kringle 1 of prothrombin. |
| Related PDB | |
| Related UniProtKB | |
| [18] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2535939 |
| Journal | Biochim Biophys Acta |
| Year | 1989 |
| Volume | 994 |
| Pages | 121-37 |
| Authors | De Marco A, Petros AM, Llinas M, Kaptein R, Boelens R |
| Title | Ligand-binding effects on the kringle 4 domain from human plasminogen: a study by laser photo-CIDNP 1H-NMR spectroscopy. |
| Related PDB | |
| Related UniProtKB | |
| [19] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1966798 |
| Journal | Blood Coagul Fibrinolysis |
| Year | 1990 |
| Volume | 1 |
| Pages | 673-9 |
| Authors | Mulichak AM, Tulinsky A |
| Title | Structure of the lysine-fibrin binding subsite of human plasminogen kringle 4. |
| Related PDB | |
| Related UniProtKB | |
| [20] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2105955 |
| Journal | J Biol Chem |
| Year | 1990 |
| Volume | 265 |
| Pages | 3906-15 |
| Authors | Thewes T, Constantine K, Byeon IJ, Llinas M |
| Title |
Ligand interactions with the kringle 5 domain of plasminogen. |
| Related PDB | |
| Related UniProtKB | |
| [21] | |
| Resource | |
| Comments | STRUCTURE BY NMR OF 374-461. |
| Medline ID | 90219023 |
| PubMed ID | 2157850 |
| Journal | J Mol Biol |
| Year | 1990 |
| Volume | 212 |
| Pages | 541-52 |
| Authors | Atkinson RA, Williams RJ |
| Title | Solution structure of the kringle 4 domain from human plasminogen by 1H nuclear magnetic resonance spectroscopy and distance geometry. |
| Related PDB | |
| Related UniProtKB | P00747 |
| [22] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 374-461. |
| Medline ID | 92031502 |
| PubMed ID | 1657148 |
| Journal | Biochemistry |
| Year | 1991 |
| Volume | 30 |
| Pages | 10576-88 |
| Authors | Mulichak AM, Tulinsky A, Ravichandran KG |
| Title | Crystal and molecular structure of human plasminogen kringle 4 refined at 1.9-A resolution. |
| Related PDB | 1pk4 |
| Related UniProtKB | P00747 |
| [23] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 374-461. |
| Medline ID | 92031503 |
| PubMed ID | 1657149 |
| Journal | Biochemistry |
| Year | 1991 |
| Volume | 30 |
| Pages | 10589-94 |
| Authors | Wu TP, Padmanabhan K, Tulinsky A, Mulichak AM |
| Title | The refined structure of the epsilon-aminocaproic acid complex of human plasminogen kringle 4. |
| Related PDB | 2pk4 |
| Related UniProtKB | P00747 |
| [24] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1847112 |
| Journal | FEBS Lett |
| Year | 1991 |
| Volume | 278 |
| Pages | 17-22 |
| Authors | Teuten AJ, Smith RA, Dobson CM |
| Title | Domain interactions in human plasminogen studied by proton NMR. |
| Related PDB | |
| Related UniProtKB | |
| [25] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1827215 |
| Journal | Thromb Haemost |
| Year | 1991 |
| Volume | 65 |
| Pages | 67-72 |
| Authors | Lu H, Soria C, Li H, Soria J, Lijnen HR, Perrot JY, Caen JP |
| Title | Role of active center and lysine binding sites of plasmin in plasmin-induced platelet activation and disaggregation. |
| Related PDB | |
| Related UniProtKB | |
| [26] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1533399 |
| Journal | J Cell Physiol |
| Year | 1992 |
| Volume | 151 |
| Pages | 255-61 |
| Authors | Ryan TJ, Lai L, Malik AB |
| Title | Plasmin generation induces neutrophil aggregation: dependence on the catalytic and lysine binding sites. |
| Related PDB | |
| Related UniProtKB | |
| [27] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8218159 |
| Journal | Biochemistry |
| Year | 1993 |
| Volume | 32 |
| Pages | 10936-43 |
| Authors | Hoover GJ, Menhart N, Martin A, Warder S, Castellino FJ |
| Title | Amino acids of the recombinant kringle 1 domain of human plasminogen that stabilize its interaction with omega-amino acids. |
| Related PDB | |
| Related UniProtKB | |
| [28] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 8054447 |
| Journal | Blood Coagul Fibrinolysis |
| Year | 1994 |
| Volume | 5 |
| Pages | 157-66 |
| Authors | Wu TP, Padmanabhan KP, Tulinsky A |
| Title | The structure of recombinant plasminogen kringle 1 and the fibrin binding site. |
| Related PDB | 1pkr |
| Related UniProtKB | |
| [29] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8187244 |
| Journal | Chem Phys Lipids |
| Year | 1994 |
| Volume | 67-68 |
| Pages | 43-58 |
| Authors | Cox M, Schaller J, Boelens R, Kaptein R, Rickli E, Llinas M |
| Title | Kringle solution structures via NMR: two-dimensional 1H-NMR analysis of horse plasminogen kringle 4. |
| Related PDB | |
| Related UniProtKB | |
| [30] | |
| Resource | |
| Comments | STRUCTURE BY NMR OF 96-184. |
| Medline ID | 94237158 |
| PubMed ID | 8181476 |
| Journal | Eur J Biochem |
| Year | 1994 |
| Volume | 221 |
| Pages | 939-49 |
| Authors | Rejante MR, Llinas M |
| Title | Solution structure of the epsilon-aminohexanoic acid complex of human plasminogen kringle 1. |
| Related PDB | 1hpj 1hpk |
| Related UniProtKB | P00747 |
| [31] | |
| Resource | |
| Comments | STRUCTURE BY NMR OF 96-184. |
| Medline ID | 94237157 |
| PubMed ID | 8181475 |
| Journal | Eur J Biochem |
| Year | 1994 |
| Volume | 221 |
| Pages | 927-37 |
| Authors | Rejante MR, Llinas M |
| Title | 1H-NMR assignments and secondary structure of human plasminogen kringle 1. |
| Related PDB | |
| Related UniProtKB | P00747 |
| [32] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 8069221 |
| Journal | Protein Sci |
| Year | 1994 |
| Volume | 3 |
| Pages | 898-910 |
| Authors | Padmanabhan K, Wu TP, Ravichandran KG, Tulinsky A |
| Title | Kringle-kringle interactions in multimer kringle structures. |
| Related PDB | 1pmk |
| Related UniProtKB | |
| [33] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7793622 |
| Journal | Anal Biochem |
| Year | 1995 |
| Volume | 226 |
| Pages | 225-31 |
| Authors | Castro MJ, Kingston IB, Anderson S |
| Title | A spectrophotometric assay for the determination of the catalytic efficiency of plasminogen activators using a slowly hydrolyzed plasmin substrate. |
| Related PDB | |
| Related UniProtKB | |
| [34] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7711052 |
| Journal | Biochim Biophys Acta |
| Year | 1995 |
| Volume | 1248 |
| Pages | 1-10 |
| Authors | Rudd PM, Woods RJ, Wormald MR, Opdenakker G, Downing AK, Campbell ID, Dwek RA |
| Title |
The effects of variable glycosylation on the functional activities of ribonuclease, |
| Related PDB | |
| Related UniProtKB | |
| [35] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 102-181. |
| Medline ID | 96180681 |
| PubMed ID | 8611560 |
| Journal | Biochemistry |
| Year | 1996 |
| Volume | 35 |
| Pages | 2567-76 |
| Authors | Mathews II, Vanderhoff-Hanaver P, Castellino FJ, Tulinsky A |
| Title | Crystal structures of the recombinant kringle 1 domain of human plasminogen in complexes with the ligands epsilon-aminocaproic acid and trans-4-(aminomethyl)cyclohexane-1-carboxylic Acid. |
| Related PDB | 1cea 1ceb |
| Related UniProtKB | P00747 |
| [36] | |
| Resource | |
| Comments | STRUCTURE BY NMR OF 183-354. |
| Medline ID | 96194156 |
| PubMed ID | 8652577 |
| Journal | Biochemistry |
| Year | 1996 |
| Volume | 35 |
| Pages | 2357-64 |
| Authors | Sohndel S, Hu CK, Marti D, Affolter M, Schaller J, Llinas M, Rickli EE |
| Title | Recombinant gene expression and 1H NMR characteristics of the kringle (2 + 3) supermodule: spectroscopic/functional individuality of plasminogen kringle domains. |
| Related PDB | |
| Related UniProtKB | P00747 |
| [37] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (1.67 ANGSTROMS) OF 376-454 |
| Medline ID | |
| PubMed ID | |
| Journal | Acta Crystallogr D Biol Crystallogr |
| Year | 1997 |
| Volume | 53 |
| Pages | 169-78 |
| Authors | Stec B, Yamano A, Whitlow M, Teeter MM |
| Title |
Structure of Human Plasminogen Kringle 4 at 1.68 angstrom and 277 K. |
| Related PDB | 1krn |
| Related UniProtKB | P00747 |
| [38] | |
| Resource | |
| Comments | PHOSPHORYLATION SITE SER-597. |
| Medline ID | 97345939 |
| PubMed ID | 9201958 |
| Journal | Biochemistry |
| Year | 1997 |
| Volume | 36 |
| Pages | 8100-6 |
| Authors | Wang H, Prorok M, Bretthauer RK, Castellino FJ |
| Title | Serine-578 is a major phosphorylation locus in human plasma plasminogen. |
| Related PDB | |
| Related UniProtKB | P00747 |
| [39] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (1.66 ANGSTROMS) OF 480-563. |
| Medline ID | 98198034 |
| PubMed ID | 9521645 |
| Journal | Biochemistry |
| Year | 1998 |
| Volume | 37 |
| Pages | 3258-71 |
| Authors | Chang Y, Mochalkin I, McCance SG, Cheng B, Tulinsky A, Castellino FJ |
| Title | Structure and ligand binding determinants of the recombinant kringle 5 domain of human plasminogen. |
| Related PDB | 5hpg |
| Related UniProtKB | P00747 |
| [40] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9563511 |
| Journal | FEBS Lett |
| Year | 1998 |
| Volume | 425 |
| Pages | 448-52 |
| Authors | Takeda-Shitaka M, Umeyama H |
| Title | Elucidation of the cause for reduced activity of abnormal human plasmin containing an Ala55-Thr mutation: importance of highly conserved Ala55 in serine proteases. |
| Related PDB | |
| Related UniProtKB | |
| [41] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 9783753 |
| Journal | Nat Struct Biol |
| Year | 1998 |
| Volume | 5 |
| Pages | 917-23 |
| Authors | Parry MA, Fernandez-Catalan C, Bergner A, Huber R, Hopfner KP, Schlott B, Guhrs KH, Bode W |
| Title | The ternary microplasmin-staphylokinase-microplasmin complex is a proteinase-cofactor-substrate complex in action. |
| Related PDB | 1bui |
| Related UniProtKB | |
| [42] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9761475 |
| Journal | Protein Sci |
| Year | 1998 |
| Volume | 7 |
| Pages | 1947-59 |
| Authors | An SS, Marti DN, Carreno C, Albericio F, Schaller J, Llinas M |
| Title | Structural/functional properties of the Glu1-HSer57 N-terminal fragment of human plasminogen: conformational characterization and interaction with kringle domains. |
| Related PDB | |
| Related UniProtKB | |
| [43] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 9733510 |
| Journal | Science |
| Year | 1998 |
| Volume | 281 |
| Pages | 1662-5 |
| Authors | Wang X, Lin X, Loy JA, Tang J, Zhang XC |
| Title | Crystal structure of the catalytic domain of human plasmin complexed with streptokinase. |
| Related PDB | 1bml |
| Related UniProtKB | |
| [44] | |
| Resource | |
| Comments | NMR Structure |
| Medline ID | |
| PubMed ID | 10625440 |
| Journal | Biochemistry |
| Year | 1999 |
| Volume | 38 |
| Pages | 15741-55 |
| Authors | Marti DN, Schaller J, Llinas M |
| Title | Solution structure and dynamics of the plasminogen kringle 2-AMCHA complex: 3(1)-helix in homologous domains. |
| Related PDB | 1b2i |
| Related UniProtKB | |
| [45] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 10460175 |
| Journal | Biochemistry |
| Year | 1999 |
| Volume | 38 |
| Pages | 11180-8 |
| Authors | Peisach E, Wang J, de los Santos T, Reich E, Ringe D |
| Title | Crystal structure of the proenzyme domain of plasminogen. |
| Related PDB | 1qrz |
| Related UniProtKB | |
| [46] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10390345 |
| Journal | J Mol Biol |
| Year | 1999 |
| Volume | 290 |
| Pages | 471-9 |
| Authors | Jespers L, Lijnen HR, Vanwetswinkel S, Van Hoef B, Brepoels K, Collen D, De Maeyer M |
| Title | Guiding a docking mode by phage display: selection of correlated mutations at the staphylokinase-plasmin interface. |
| Related PDB | |
| Related UniProtKB | |
| [47] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11076540 |
| Journal | Biochemistry |
| Year | 2000 |
| Volume | 39 |
| Pages | 13974-81 |
| Authors | Boxrud PD, Bock PE |
| Title | Streptokinase binds preferentially to the extended conformation of plasminogen through lysine binding site and catalytic domain interactions. |
| Related PDB | |
| Related UniProtKB | |
| [48] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10769130 |
| Journal | Biochemistry |
| Year | 2000 |
| Volume | 39 |
| Pages | 4740-5 |
| Authors | Lin LF, Houng A, Reed GL |
| Title | Epsilon amino caproic acid inhibits streptokinase-plasminogen activator complex formation and substrate binding through kringle-dependent mechanisms. |
| Related PDB | |
| Related UniProtKB | |
| [49] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 10656799 |
| Journal | J Mol Biol |
| Year | 2000 |
| Volume | 295 |
| Pages | 903-14 |
| Authors | Wang X, Terzyan S, Tang J, Loy JA, Lin X, Zhang XC |
| Title | Human plasminogen catalytic domain undergoes an unusual conformational change upon activation. |
| Related PDB | 1ddj |
| Related UniProtKB | |
| [50] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10926513 |
| Journal | J Mol Biol |
| Year | 2000 |
| Volume | 301 |
| Pages | 333-47 |
| Authors | Zajicek J, Chang Y, Castellino FJ |
| Title | The effects of ligand binding on the backbone dynamics of the kringle 1 domain of human plasminogen. |
| Related PDB | |
| Related UniProtKB | |
| [51] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11152303 |
| Journal | J Pept Res |
| Year | 2000 |
| Volume | 56 |
| Pages | 438-45 |
| Authors | Schenone MM, Warder SE, Martin JA, Prorok M, Castellino FJ |
| Title |
An internal histidine residue from the bacterial surface protein, |
| Related PDB | |
| Related UniProtKB | |
| [52] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 11350170 |
| Journal | J Mol Biol |
| Year | 2001 |
| Volume | 308 |
| Pages | 705-19 |
| Authors | Rios-Steiner JL, Schenone M, Mochalkin I, Tulinsky A, Castellino FJ |
| Title | Structure and binding determinants of the recombinant kringle-2 domain of human plasminogen to an internal peptide from a group A Streptococcal surface protein. |
| Related PDB | 1i5k |
| Related UniProtKB | |
| [53] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12080056 |
| Journal | J Biol Chem |
| Year | 2002 |
| Volume | 277 |
| Pages | 33068-74 |
| Authors | Turner RB, Liu L, Sazonova IY, Reed GL |
| Title | Structural elements that govern the substrate specificity of the clot-dissolving enzyme plasmin. |
| Related PDB | |
| Related UniProtKB | |
| [54] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 12054798 |
| Journal | J Mol Biol |
| Year | 2002 |
| Volume | 318 |
| Pages | 1009-17 |
| Authors | Abad MC, Arni RK, Grella DK, Castellino FJ, Tulinsky A, Geiger JH |
| Title | The X-ray crystallographic structure of the angiogenesis inhibitor angiostatin. |
| Related PDB | 1ki0 |
| Related UniProtKB | |
| [55] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 12456874 |
| Journal | Protein Eng |
| Year | 2002 |
| Volume | 15 |
| Pages | 753-61 |
| Authors | Wakeham N, Terzyan S, Zhai P, Loy JA, Tang J, Zhang XC |
| Title | Effects of deletion of streptokinase residues 48-59 on plasminogen activation. |
| Related PDB | 1l4d |
| Related UniProtKB | |
| [56] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12515545 |
| Journal | Biochemistry |
| Year | 2003 |
| Volume | 42 |
| Pages | 114-20 |
| Authors | Zhai P, Wakeham N, Loy JA, Zhang XC |
| Title | Functional roles of streptokinase C-terminal flexible peptide in active site formation and substrate recognition in plasminogen activation. |
| Related PDB | |
| Related UniProtKB | |
| [57] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12773528 |
| Journal | J Biol Chem |
| Year | 2003 |
| Volume | 278 |
| Pages | 30569-77 |
| Authors | Sundram V, Nanda JS, Rajagopal K, Dhar J, Chaudhary A, Sahni G |
| Title | Domain truncation studies reveal that the streptokinase-plasmin activator complex utilizes long range protein-protein interactions with macromolecular substrate to maximize catalytic turnover. |
| Related PDB | |
| Related UniProtKB | |
| [58] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 15211511 |
| Journal | Proteins |
| Year | 2004 |
| Volume | 56 |
| Pages | 277-84 |
| Authors | Terzyan S, Wakeham N, Zhai P, Rodgers K, Zhang XC |
| Title | Characterization of Lys-698-to-Met substitution in human plasminogen catalytic domain. |
| Related PDB | 1rjx |
| Related UniProtKB | |
| Comments |
|---|
|
This enzyme is composed of N-terminal PAN domain, Although this enzyme has got a classical catalytic triad, Thus, |
| Created | Updated |
|---|---|
| 2004-08-20 | 2011-02-17 |