DB code: D00224
| RLCP classification | 1.13.30000.10 : Hydrolysis | |
|---|---|---|
| CATH domain | 2.40.10.10 : Thrombin, subunit H | Catalytic domain |
| 2.40.10.10 : Thrombin, subunit H | Catalytic domain | |
| E.C. | 3.4.21.81 | |
| CSA | 1ds2 | |
| M-CSA | 1ds2 | |
| MACiE | ||
| CATH domain | Related DB codes (homologues) |
|---|---|
| 2.40.10.10 : Thrombin, subunit H | M00139 D00214 M00167 D00426 M00133 D00428 D00429 D00430 D00431 D00432 D00433 D00434 D00435 M00227 M00209 D00194 D00197 D00211 D00212 D00216 M00212 D00497 M00217 M00216 D00528 D00848 D00850 D00851 D00852 D00855 M00152 M00155 M00157 M00181 M00315 M00316 M00317 M00348 M00349 T00074 T00410 T00411 |
| Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | MEROPS | Pfam |
|---|---|---|---|---|
| P00777 |
Streptogrisin-B
|
EC
3.4.21.81
Serine protease B SGPB Pronase enzyme B |
S01.262
(Serine)
|
PF02983
(Pro_Al_protease)
PF00089 (Trypsin) [Graphical View] |
| KEGG enzyme name |
|---|
|
streptogrisin B
Streptomyces griseus protease B pronase B serine proteinase B Streptomyces griseus proteinase B Streptomyces griseus proteinase 1 Streptomyces griseus serine proteinase B |
| UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
|---|---|---|---|---|---|
| P00777 | PRTB_STRGR | Hydrolysis of proteins with trypsin-like specificity. | Monomer. |
| KEGG Pathways | Map code | Pathways | E.C. |
|---|
| Compound table | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Substrates | Products | Intermediates | |||||||||||
| KEGG-id | C00017 | C00012 | C00001 | C00017 | C00012 | I00087 | I00085 | I00086 | |||||
| E.C. | |||||||||||||
| Compound | Protein | Peptide | H2O | Protein | Peptide | Peptidyl-tetrahedral intermediate | Acyl-enzyme | Tetrahedral intermediate | |||||
| Type | peptide/protein | peptide/protein | H2O | peptide/protein | peptide/protein | ||||||||
| ChEBI |
15377 15377 |
||||||||||||
| PubChem |
22247451 962 22247451 962 |
||||||||||||
| 1csoE01 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ct0E01 |
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|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ct2E01 |
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|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ct4E01 |
|
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|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ds2E01 |
|
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|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1sgdE01 |
|
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|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1sgeE01 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1sgnE01 |
|
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|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1sgpE01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1sgqE01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1sgrE01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1sgyE01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2sgdE01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2sgeE01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2sgfE01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2sgpE01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2sgqE01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 3sgbE01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 3sgqE01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 4sgbE01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1csoE02 |
|
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Unbound | Analogue:ILE_18-GLU_19(chain I) | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ct0E02 |
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Unbound | Analogue:SER_18-GLU_19(chain I) | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ct2E02 |
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Unbound | Analogue:THR_18-GLU_19(chain I) | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ct4E02 |
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Unbound | Analogue:VAL_18-GLU_19(chain I) | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ds2E02 |
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Unbound | Analogue:1LU_18-GLU_19(chain I) | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1sgdE02 |
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Unbound | Analogue:ASP_18-GLU_19(chain I) | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1sgeE02 |
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Unbound | Analogue:GLU_18-GLU_19(chain I) | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1sgnE02 |
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Unbound | Analogue:ASN_18-GLU_19(chain I) | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1sgpE02 |
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Unbound | Analogue:ALA_18-GLU_19(chain I) | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1sgqE02 |
|
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Unbound | Analogue:GLY_18-GLU_19(chain I) | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1sgrE02 |
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Unbound | Analogue:LEU_18-GLU_19(chain I) | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1sgyE02 |
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Unbound | Analogue:TYR_18-GLU_19(chain I) | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2sgdE02 |
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Unbound | Analogue:ASP_18-GLU_19(chain I) | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2sgeE02 |
|
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Unbound | Analogue:GLU_18-GLU_19(chain I) | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2sgfE02 |
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Unbound | Analogue:PHE_18-GLU_19(chain I) | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2sgpE02 |
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Unbound | Analogue:PRO_18-GLU_19(chain I) | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2sgqE02 |
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Unbound | Analogue:GLN_18-GLU_19(chain I) | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 3sgbE02 |
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Unbound | Analogue:LEU_18-GLU_19(chain I) | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 3sgqE02 |
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Unbound | Analogue:GLN_18-GLU_19(chain I) | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 4sgbE02 |
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Unbound | Analogue:LEU_38-ASN_39(chain I) | Unbound | Unbound | Unbound | Unbound | Unbound | |
| Reference for Active-site residues | ||
|---|---|---|
| resource | references | E.C. |
| PDB;1sgd, 1sge, 1sgn, 1sgp,1sgq,1sgr, 2sgd, 2sge, 2sgf, 2sgp, 2sgq, 3sgq & Swiss-prot;P00777 & literature [3], [5], [7] | ||
| Active-site residues | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| PDB | Catalytic residues | Cofactor-binding residues | Modified residues | Main-chain involved in catalysis | Comment | |||||
| 1csoE01 |
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HIS 57;ASP 102 | ||||
| 1ct0E01 |
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HIS 57;ASP 102 | ||||
| 1ct2E01 |
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HIS 57;ASP 102 | ||||
| 1ct4E01 |
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HIS 57;ASP 102 | ||||
| 1ds2E01 |
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HIS 57;ASP 102 | ||||
| 1sgdE01 |
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HIS 57;ASP 102 | ||||
| 1sgeE01 |
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HIS 57;ASP 102 | ||||
| 1sgnE01 |
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HIS 57;ASP 102 | ||||
| 1sgpE01 |
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HIS 57;ASP 102 | ||||
| 1sgqE01 |
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HIS 57;ASP 102 | ||||
| 1sgrE01 |
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HIS 57;ASP 102 | ||||
| 1sgyE01 |
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HIS 57;ASP 102 | ||||
| 2sgdE01 |
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HIS 57;ASP 102 | ||||
| 2sgeE01 |
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HIS 57;ASP 102 | ||||
| 2sgfE01 |
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HIS 57;ASP 102 | ||||
| 2sgpE01 |
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HIS 57;ASP 102 | ||||
| 2sgqE01 |
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HIS 57;ASP 102 | ||||
| 3sgbE01 |
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HIS 57;ASP 102 | ||||
| 3sgqE01 |
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HIS 57;ASP 102 | ||||
| 4sgbE01 |
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HIS 57;ASP 102 | ||||
| 1csoE02 |
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SER 195 | GLY 193;SER 195 | |||
| 1ct0E02 |
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SER 195 | GLY 193;SER 195 | |||
| 1ct2E02 |
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SER 195 | GLY 193;SER 195 | |||
| 1ct4E02 |
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SER 195 | GLY 193;SER 195 | |||
| 1ds2E02 |
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SER 195 | GLY 193;SER 195 | |||
| 1sgdE02 |
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SER 195 | GLY 193;SER 195 | |||
| 1sgeE02 |
|
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SER 195 | GLY 193;SER 195 | |||
| 1sgnE02 |
|
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SER 195 | GLY 193;SER 195 | |||
| 1sgpE02 |
|
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SER 195 | GLY 193;SER 195 | |||
| 1sgqE02 |
|
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SER 195 | GLY 193;SER 195 | |||
| 1sgrE02 |
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SER 195 | GLY 193;SER 195 | |||
| 1sgyE02 |
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SER 195 | GLY 193;SER 195 | |||
| 2sgdE02 |
|
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SER 195 | GLY 193;SER 195 | |||
| 2sgeE02 |
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SER 195 | GLY 193;SER 195 | |||
| 2sgfE02 |
|
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SER 195 | GLY 193;SER 195 | |||
| 2sgpE02 |
|
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|
SER 195 | GLY 193;SER 195 | |||
| 2sgqE02 |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 3sgbE02 |
|
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|
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|
SER 195 | GLY 193;SER 195 | |||
| 3sgqE02 |
|
|
|
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|
SER 195 | GLY 193;SER 195 | |||
| 4sgbE02 |
|
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SER 195 | GLY 193;SER 195 | |||
| References for Catalytic Mechanism | ||
|---|---|---|
| References | Sections | No. of steps in catalysis |
|
[5]
|
p.142 | |
|
[7]
|
p.431 | |
|
[9]
|
||
| References | |
|---|---|
| [1] | |
| Resource | |
| Comments | ACTIVE SITE. |
| Medline ID | 68124983 |
| PubMed ID | 5636372 |
| Journal | Biochim Biophys Acta |
| Year | 1968 |
| Volume | 151 |
| Pages | 402-8 |
| Authors | Wahlby S, Engstrom L |
| Title |
Studies on Streptomyces griseus protease. |
| Related PDB | |
| Related UniProtKB | P00777 |
| [2] | |
| Resource | |
| Comments | ACTIVE SITE. |
| Medline ID | 74302902 |
| PubMed ID | 4212092 |
| Journal | FEBS Lett |
| Year | 1974 |
| Volume | 43 |
| Pages | 81-5 |
| Authors | Gertler A |
| Title | Inhibition of Streptomyces griseus protease B by peptide chloromethyl ketones: partial mapping of the binding site and identification of the reactive residue. |
| Related PDB | |
| Related UniProtKB | P00777 |
| [3] | |
| Resource | |
| Comments |
X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS), |
| Medline ID | 76051298 |
| PubMed ID | 1186854 |
| Journal | Nature |
| Year | 1975 |
| Volume | 257 |
| Pages | 758-63 |
| Authors | Delbaere LT, Hutcheon WL, James MN, Thiessen WE |
| Title | Tertiary structural differences between microbial serine proteases and pancreatic serine enzymes. |
| Related PDB | |
| Related UniProtKB | P00777 |
| [4] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 413567 |
| Journal | Biochemistry |
| Year | 1978 |
| Volume | 17 |
| Pages | 375-80 |
| Authors | Bauer CA |
| Title |
Active centers of Streptomyces griseus protease 1, |
| Related PDB | |
| Related UniProtKB | |
| [5] | |
| Resource | |
| Comments |
X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS), |
| Medline ID | 79210651 |
| PubMed ID | 110426 |
| Journal | Can J Biochem |
| Year | 1979 |
| Volume | 57 |
| Pages | 135-44 |
| Authors | Delbaere LT, Brayer GD, James MN |
| Title | The 2.8 A resolution structure of Streptomyces griseus protease B and its homology with alpha-chymotrypsin and Streptomyces griseus protease A. |
| Related PDB | |
| Related UniProtKB | P00777 |
| [6] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 6769571 |
| Journal | Can J Biochem |
| Year | 1980 |
| Volume | 58 |
| Pages | 252-71 |
| Authors | James MN |
| Title | An X-ray crystallographic approach to enzyme structure and function. |
| Related PDB | |
| Related UniProtKB | |
| [7] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 6777499 |
| Journal | J Mol Biol |
| Year | 1980 |
| Volume | 139 |
| Pages | 423-38 |
| Authors | James MN, Brayer GD, Delbaere LT, Sielecki AR, Gertler A |
| Title | Crystal structure studies and inhibition kinetics of tripeptide chloromethyl ketone inhibitors with Streptomyces griseus protease B. |
| Related PDB | |
| Related UniProtKB | |
| [8] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF THIRD DOMAIN. |
| Medline ID | 83014993 |
| PubMed ID | 6750612 |
| Journal | Proc Natl Acad Sci U S A |
| Year | 1982 |
| Volume | 79 |
| Pages | 4868-72 |
| Authors | Fujinaga M, Read RJ, Sielecki A, Ardelt W, Laskowski M Jr, James MN |
| Title |
Refined crystal structure of the molecular complex of Streptomyces griseus protease B, |
| Related PDB | |
| Related UniProtKB | |
| [9] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 6414511 |
| Journal | Biochemistry |
| Year | 1983 |
| Volume | 22 |
| Pages | 4420-33 |
| Authors | Read RJ, Fujinaga M, Sielecki AR, James MN |
| Title | Structure of the complex of Streptomyces griseus protease B and the third domain of the turkey ovomucoid inhibitor at 1.8-A resolution. |
| Related PDB | 3sgb |
| Related UniProtKB | |
| [10] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 55-106. |
| Medline ID | 89178636 |
| PubMed ID | 2494344 |
| Journal | J Mol Biol |
| Year | 1989 |
| Volume | 205 |
| Pages | 201-28 |
| Authors | Greenblatt HM, Ryan CA, James MN |
| Title | Structure of the complex of Streptomyces griseus proteinase B and polypeptide chymotrypsin inhibitor-1 from Russet Burbank potato tubers at 2.1 A resolution. |
| Related PDB | 4sgb |
| Related UniProtKB | P00777 |
| [11] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8495199 |
| Journal | Protein Sci |
| Year | 1993 |
| Volume | 2 |
| Pages | 786-99 |
| Authors | Bigler TL, Lu W, Park SJ, Tashiro M, Wieczorek M, Wynn R, Laskowski M Jr |
| Title | Binding of amino acid side chains to preformed cavities: interaction of serine proteinases with turkey ovomucoid third domains with coded and noncoded P1 residues. |
| Related PDB | |
| Related UniProtKB | |
| [12] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7499197 |
| Journal | J Biol Chem |
| Year | 1995 |
| Volume | 270 |
| Pages | 27419-22 |
| Authors | Abul Qasim M, Ranjbar MR, Wynn R, Anderson S, Laskowski M Jr |
| Title | Ionizable P1 residues in serine proteinase inhibitors undergo large pK shifts on complex formation. |
| Related PDB | |
| Related UniProtKB | |
| [13] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 8535235 |
| Journal | Protein Sci |
| Year | 1995 |
| Volume | 4 |
| Pages | 1985-97 |
| Authors | Huang K, Lu W, Anderson S, Laskowski M Jr, James MN |
| Title |
Water molecules participate in proteinase-inhibitor interactions: crystal structures of Leu18, |
| Related PDB | 1sgp 1sgq 1sgr |
| Related UniProtKB | |
| [14] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9020764 |
| Journal | Biochemistry |
| Year | 1997 |
| Volume | 36 |
| Pages | 673-9 |
| Authors | Lu W, Qasim MA, Laskowski M Jr, Kent SB |
| Title | Probing intermolecular main chain hydrogen bonding in serine proteinase-protein inhibitor complexes: chemical synthesis of backbone-engineered turkey ovomucoid third domain. |
| Related PDB | |
| Related UniProtKB | |
| [15] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9878379 |
| Journal | J Mol Biol |
| Year | 1998 |
| Volume | 284 |
| Pages | 1683-94 |
| Authors | Fujinaga M, Huang K, Bateman KS, James MN |
| Title | Computational analysis of the binding of P1 variants of domain 3 of turkey ovomucoid inhibitor to Streptomyces griseus protease B. |
| Related PDB | |
| Related UniProtKB | |
| [16] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10712933 |
| Journal | Chem Biol |
| Year | 2000 |
| Volume | 7 |
| Pages | 163-71 |
| Authors | Elliott RJ, Bennet AJ, Braun CA, MacLeod AM, Borgford TJ |
| Title | Active-site variants of Streptomyces griseus protease B with peptide-ligation activity. |
| Related PDB | |
| Related UniProtKB | |
| [17] | |
| Resource | |
| Comments | X-RAY DIFFRACTION |
| Medline ID | |
| PubMed ID | 10739250 |
| Journal | Protein Sci |
| Year | 2000 |
| Volume | 9 |
| Pages | 83-94 |
| Authors | Bateman KS, Anderson S, Lu W, Qasim MA, Laskowski M Jr, James MN |
| Title |
Deleterious effects of beta-branched residues in the S1 specificity pocket of Streptomyces griseus proteinase B (SGPB): crystal structures of the turkey ovomucoid third domain variants Ile18I, |
| Related PDB | 1cso 1ct0 1ct2 1ct4 |
| Related UniProtKB | |
| [18] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 11162096 |
| Journal | J Mol Biol |
| Year | 2001 |
| Volume | 305 |
| Pages | 839-49 |
| Authors | Bateman KS, Huang K, Anderson S, Lu W, Qasim MA, Laskowski M Jr, James MN |
| Title |
Contribution of peptide bonds to inhibitor-protease binding: crystal structures of the turkey ovomucoid third domain backbone variants OMTKY3-Pro18I and OMTKY3-psi[COO]-Leu18I in complex with Streptomyces griseus proteinase B (SGPB) and the structure of the free inhibitor, |
| Related PDB | 1ds2 2sgp |
| Related UniProtKB | |
| [19] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 14718653 |
| Journal | Protein Sci |
| Year | 2004 |
| Volume | 13 |
| Pages | 381-90 |
| Authors | Truhlar SM, Cunningham EL, Agard DA |
| Title | The folding landscape of Streptomyces griseus protease B reveals the energetic costs and benefits associated with evolving kinetic stability. |
| Related PDB | |
| Related UniProtKB | |
| [20] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 15740746 |
| Journal | J Mol Biol |
| Year | 2005 |
| Volume | 347 |
| Pages | 355-66 |
| Authors | Jaswal SS, Truhlar SM, Dill KA, Agard DA |
| Title | Comprehensive analysis of protein folding activation thermodynamics reveals a universal behavior violated by kinetically stable proteases. |
| Related PDB | |
| Related UniProtKB | |
| Comments |
|---|
|
This enzyme belongs to the peptidase family-S1E.
This enzyme has got a catalytic triad composed of Ser/His/Asp, |
| Created | Updated |
|---|---|
| 2003-10-14 | 2011-02-21 |