DB code: M00315
| RLCP classification | 1.13.30000.10 : Hydrolysis | |
|---|---|---|
| CATH domain | 2.60.120.290 : Jelly Rolls | |
| 2.10.25.10 : Laminin | ||
| 2.60.120.290 : Jelly Rolls | ||
| 2.10.70.10 : Complement Module; domain 1 | ||
| 2.10.70.10 : Complement Module; domain 1 | ||
| 2.40.10.10 : Thrombin, subunit H | Catalytic domain | |
| 2.40.10.10 : Thrombin, subunit H | Catalytic domain | |
| E.C. | 3.4.21.104 | |
| CSA | ||
| M-CSA | ||
| MACiE | ||
| CATH domain | Related DB codes (homologues) |
|---|---|
| 2.10.25.10 : Laminin | M00139 M00133 M00212 M00152 M00155 M00316 |
| 2.10.70.10 : Complement Module; domain 1 | M00139 M00155 M00316 |
| 2.40.10.10 : Thrombin, subunit H | M00139 D00214 M00167 D00426 M00133 D00428 D00429 D00430 D00431 D00432 D00433 D00434 D00435 M00227 M00209 D00194 D00197 D00211 D00212 D00216 M00212 D00224 D00497 M00217 M00216 D00528 D00848 D00850 D00851 D00852 D00855 M00152 M00155 M00157 M00181 M00316 M00317 M00348 M00349 T00074 T00410 T00411 |
| 2.60.120.290 : Jelly Rolls | M00139 M00227 M00316 M00317 M00348 |
| Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | Contains | RefSeq | MEROPS | Pfam |
|---|---|---|---|---|---|---|
| Q9JJS8 |
Mannan-binding lectin serine protease 2
|
EC
3.4.21.104
MBL-associated serine protease 2 Mannose-binding protein-associated serine protease 2 MASP-2 |
Mannan-binding lectin serine protease 2 A chain
Mannan-binding lectin serine protease 2 B chain |
NP_742040.1
(Protein)
NM_172043.1 (DNA/RNA sequence) |
S01.229
(Serine)
|
PF00431
(CUB)
PF07645 (EGF_CA) PF00084 (Sushi) PF00089 (Trypsin) [Graphical View] |
| O00187 |
Mannan-binding lectin serine protease 2
|
EC
3.4.21.104
MBL-associated serine protease 2 Mannose-binding protein-associated serine protease 2 MASP-2 |
Mannan-binding lectin serine protease 2 A chain
Mannan-binding lectin serine protease 2 B chain |
NP_006601.2
(Protein)
NM_006610.3 (DNA/RNA sequence) NP_631947.1 (Protein) NM_139208.2 (DNA/RNA sequence) |
S01.229
(Serine)
|
PF00431
(CUB)
PF07645 (EGF_CA) PF00084 (Sushi) PF00089 (Trypsin) [Graphical View] |
| KEGG enzyme name |
|---|
|
Mannan-binding lectin-associated serine protease-2
MASP-2 MASP2 MBP-associated serine protease-2 Mannose-binding lectin-associated serine protease-2 p100 Mannan-binding lectin-associated serine peptidase 2 |
| UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
|---|---|---|---|---|---|
| Q9JJS8 | MASP2_RAT | Selective cleavage after Arg-223 in complement component C2 (-Ser-Leu-Gly-Arg-|-Lys-Ile-Gln-Ile) and after Arg-76 in complement component C4 (-Gly-Leu-Gln-Arg-|-Ala-Leu-Glu-Ile). | Homodimer. disulfide-linked. Binds MBL2. Isoform 2 binds to MASP1. Binds SERPING1 By similarity. | Secreted. | |
| O00187 | MASP2_HUMAN | Selective cleavage after Arg-223 in complement component C2 (-Ser-Leu-Gly-Arg-|-Lys-Ile-Gln-Ile) and after Arg-76 in complement component C4 (-Gly-Leu-Gln-Arg-|-Ala-Leu-Glu-Ile). | Homodimer. disulfide-linked. Binds MBL2. Isoform 2 binds to MASP1. Binds SERPING1. Dimerization and MBL2 binding requires calcium ions. of C3. Interacts with SERPING1. | Secreted. |
| KEGG Pathways | Map code | Pathways | E.C. |
|---|
| Compound table | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Substrates | Products | Intermediates | |||||||||||||
| KEGG-id | L00082 | L00083 | C00001 | L00084 | L00085 | L00086 | L00080 | I00087 | I00085 | I00086 | |||||
| E.C. | |||||||||||||||
| Compound | Complement component C2 | Complement component C4 | H2O | Complement component C2a | Complement component C2b | Complement component C4a | Complement component C4b | Peptidyl-Ser-tetrahedral-intermediate (with previous peptide) | Acyl-enzyme(Peptidyl-Ser-acyl group) | Peptidyl-Ser-tetrahedral-intermediate | |||||
| Type | peptide/protein | peptide/protein | H2O | peptide/protein | peptide/protein | peptide/protein | peptide/protein | ||||||||
| ChEBI |
15377 15377 |
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| PubChem |
22247451 962 22247451 962 |
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| 1nt0A01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1nt0G01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1szbA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1szbB01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1nt0A02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1nt0G02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1szbA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1szbB02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1nt0A03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1nt0G03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1zjkA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1q3xA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1q3xB01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1zjkA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 3tvjA |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1q3xA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1q3xB02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1zjkA03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 3tvjB01 |
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Unbound | Analogue:LYS_30-LEU_31(chain I) | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1q3xA03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1q3xB03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1zjkA04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 3tvjB02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| Reference for Active-site residues | ||
|---|---|---|
| resource | references | E.C. |
| Literature [11], [14] & Swiss-prot;O00187 | ||
| Active-site residues | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| PDB | Catalytic residues | Cofactor-binding residues | Modified residues | Main-chain involved in catalysis | Comment | |||||
| 1nt0A01 |
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| 1nt0G01 |
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| 1szbA01 |
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| 1szbB01 |
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| 1nt0A02 |
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| 1nt0G02 |
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| 1szbA02 |
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| 1szbB02 |
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| 1nt0A03 |
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| 1nt0G03 |
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| 1zjkA01 |
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| 1q3xA01 |
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| 1q3xB01 |
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| 1zjkA02 |
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| 3tvjA |
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| 1q3xA02 |
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SER 633 | GLY 631;SER 633 | |||
| 1q3xB02 |
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SER 633 | GLY 631;SER 633 | |||
| 1zjkA03 |
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SER 633 | GLY 631;SER 633 | |||
| 3tvjB01 |
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SER 633 | GLY 631;SER 633 | |||
| 1q3xA03 |
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HIS 483;ASP 532 | ||||
| 1q3xB03 |
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HIS 483;ASP 532 | ||||
| 1zjkA04 |
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HIS 483;ASP 532 | ||||
| 3tvjB02 |
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HIS 483;ASP 532 | ||||
| References for Catalytic Mechanism | ||
|---|---|---|
| References | Sections | No. of steps in catalysis |
|
[5]
|
Figure 5, p.4505-4508 | |
| References | |
|---|---|
| [1] | |
| Resource | |
| Comments |
FUNCTION, |
| Medline ID | |
| PubMed ID | 10946292 |
| Journal | J Immunol |
| Year | 2000 |
| Volume | 165 |
| Pages | 2637-42 |
| Authors | Matsushita M, Thiel S, Jensenius JC, Terai I, Fujita T |
| Title | Proteolytic activities of two types of mannose-binding lectin-associated serine protease. |
| Related PDB | |
| Related UniProtKB | |
| [2] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10925294 |
| Journal | J Immunol |
| Year | 2000 |
| Volume | 165 |
| Pages | 2093-100 |
| Authors | Vorup-Jensen T, Petersen SV, Hansen AG, Poulsen K, Schwaeble W, Sim RB, Reid KB, Davis SJ, Thiel S, Jensenius JC |
| Title | Distinct pathways of mannan-binding lectin (MBL)- and C1-complex autoactivation revealed by reconstitution of MBL with recombinant MBL-associated serine protease-2. |
| Related PDB | |
| Related UniProtKB | |
| [3] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12369900 |
| Journal | Curr Protein Pept Sci |
| Year | 2001 |
| Volume | 2 |
| Pages | 43-59 |
| Authors | Gal P, Ambrus G |
| Title | Structure and function of complement activating enzyme complexes: C1 and MBL-MASPs. |
| Related PDB | |
| Related UniProtKB | |
| [4] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11527969 |
| Journal | J Biol Chem |
| Year | 2001 |
| Volume | 276 |
| Pages | 40880-7 |
| Authors | Rossi V, Cseh S, Bally I, Thielens NM, Jensenius JC, Arlaud GJ |
| Title | Substrate specificities of recombinant mannan-binding lectin-associated serine proteases-1 and -2. |
| Related PDB | |
| Related UniProtKB | |
| [5] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12475199 |
| Journal | Chem Rev |
| Year | 2002 |
| Volume | 102 |
| Pages | 4501-24 |
| Authors | Hedstrom L |
| Title | Serine protease mechanism and specificity. |
| Related PDB | |
| Related UniProtKB | |
| [6] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12396007 |
| Journal | Immunobiology |
| Year | 2002 |
| Volume | 205 |
| Pages | 455-66 |
| Authors | Schwaeble W, Dahl MR, Thiel S, Stover C, Jensenius JC |
| Title | The mannan-binding lectin-associated serine proteases (MASPs) and MAp19: four components of the lectin pathway activation complex encoded by two genes. |
| Related PDB | |
| Related UniProtKB | |
| [7] | |
| Resource | |
| Comments |
X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 20-299, |
| Medline ID | |
| PubMed ID | 12743029 |
| Journal | EMBO J |
| Year | 2003 |
| Volume | 22 |
| Pages | 2348-59 |
| Authors | Feinberg H, Uitdehaag JC, Davies JM, Wallis R, Drickamer K, Weis WI |
| Title | Crystal structure of the CUB1-EGF-CUB2 region of mannose-binding protein associated serine protease-2. |
| Related PDB | 1nt0 |
| Related UniProtKB | Q9JJS8 |
| [8] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12538697 |
| Journal | J Immunol |
| Year | 2003 |
| Volume | 170 |
| Pages | 1374-82 |
| Authors | Ambrus G, Gal P, Kojima M, Szilagyi K, Balczer J, Antal J, Graf L, Laich A, Moffatt BE, Schwaeble W, Sim RB, Zavodszky P |
| Title | Natural substrates and inhibitors of mannan-binding lectin-associated serine protease-1 and -2: a study on recombinant catalytic fragments. |
| Related PDB | |
| Related UniProtKB | |
| [9] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 15060079 |
| Journal | J Biol Chem |
| Year | 2004 |
| Volume | 279 |
| Pages | 26058-65 |
| Authors | Chen CB, Wallis R |
| Title | Two mechanisms for mannose-binding protein modulation of the activity of its associated serine proteases. |
| Related PDB | |
| Related UniProtKB | |
| [10] | |
| Resource | |
| Comments |
X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 16-181 (ISOFORM 2), |
| Medline ID | |
| PubMed ID | 15117939 |
| Journal | J Biol Chem |
| Year | 2004 |
| Volume | 279 |
| Pages | 29391-7 |
| Authors | Gregory LA, Thielens NM, Matsushita M, Sorensen R, Arlaud GJ, Fontecilla-Camps JC, Gaboriaud C |
| Title | The X-ray structure of human mannan-binding lectin-associated protein 19 (MAp19) and its interaction site with mannan-binding lectin and L-ficolin. |
| Related PDB | 1szb |
| Related UniProtKB | O00187 |
| [11] | |
| Resource | |
| Comments |
X-RAY CRYSTALLOGRAPHY (2.23 ANGSTROMS) OF 363-686, |
| Medline ID | |
| PubMed ID | 15364579 |
| Journal | J Mol Biol |
| Year | 2004 |
| Volume | 342 |
| Pages | 1533-46 |
| Authors | Harmat V, Gal P, Kardos J, Szilagyi K, Ambrus G, Vegh B, Naray-Szabo G, Zavodszky P |
| Title | The structure of MBL-associated serine protease-2 reveals that identical substrate specificities of C1s and MASP-2 are realized through different sets of enzyme-substrate interactions. |
| Related PDB | 1q3x |
| Related UniProtKB | O00187 |
| [12] | |
| Resource | |
| Comments |
X-RAY CRYSTALLOGRAPHY (2.18 ANGSTROMS) OF 287-686, |
| Medline ID | |
| PubMed ID | 16040602 |
| Journal | J Biol Chem |
| Year | 2005 |
| Volume | 280 |
| Pages | 33435-44 |
| Authors | Gal P, Harmat V, Kocsis A, Bian T, Barna L, Ambrus G, Vegh B, Balczer J, Sim RB, Naray-Szabo G, Zavodszky P |
| Title |
A true autoactivating enzyme. |
| Related PDB | 1zjk |
| Related UniProtKB | O00187 |
| [13] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 17709141 |
| Journal | Mol Immunol |
| Year | 2008 |
| Volume | 45 |
| Pages | 670-7 |
| Authors | Kerr FK, Thomas AR, Wijeyewickrema LC, Whisstock JC, Boyd SE, Kaiserman D, Matthews AY, Bird PI, Thielens NM, Rossi V, Pike RN |
| Title |
Elucidation of the substrate specificity of the MASP-2 protease of the lectin complement pathway and identification of the enzyme as a major physiological target of the serpin, |
| Related PDB | |
| Related UniProtKB | |
| [14] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 19477526 |
| Journal | Mol Immunol |
| Year | 2009 |
| Volume | 46 |
| Pages | 2745-52 |
| Authors | Gal P, Dobo J, Zavodszky P, Sim RB |
| Title |
Early complement proteases: C1r, |
| Related PDB | |
| Related UniProtKB | |
| [15] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 22511776 |
| Journal | J Biol Chem |
| Year | 2012 |
| Volume | 287 |
| Pages | 20290-300 |
| Authors | Heja D, Harmat V, Fodor K, Wilmanns M, Dobo J, Kekesi KA, Zavodszky P, Gal P, Pal G |
| Title | Monospecific inhibitors show that both mannan-binding lectin-associated serine protease-1 (MASP-1) and -2 Are essential for lectin pathway activation and reveal structural plasticity of MASP-2. |
| Related PDB | 3tvj |
| Related UniProtKB | |
| Comments |
|---|
|
This enzyme belongs to peptidase family-S1.
This enzyme (MASP-2) can activate C2 and C4 in the lectin pathway of the complement system (see [3], This enzyme is involved in "lectin pathway" of complement system, Since this enzyme has got the same catalytic site as trypsin, |
| Created | Updated |
|---|---|
| 2011-02-24 | 2012-08-10 |