DB code: D00194
| RLCP classification | 1.13.30000.10 : Hydrolysis | |
|---|---|---|
| CATH domain | 2.40.10.10 : Thrombin, subunit H | Catalytic domain |
| 2.40.10.10 : Thrombin, subunit H | Catalytic domain | |
| E.C. | 3.4.21.1 | |
| CSA | 1ca0 1hja | |
| M-CSA | 1ca0 1hja | |
| MACiE | ||
| CATH domain | Related DB codes (homologues) |
|---|---|
| 2.40.10.10 : Thrombin, subunit H | M00139 D00214 M00167 D00426 M00133 D00428 D00429 D00430 D00431 D00432 D00433 D00434 D00435 M00227 M00209 D00197 D00211 D00212 D00216 M00212 D00224 D00497 M00217 M00216 D00528 D00848 D00850 D00851 D00852 D00855 M00152 M00155 M00157 M00181 M00315 M00316 M00317 M00348 M00349 T00074 T00410 T00411 |
| Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | Contains | Pfam | MEROPS |
|---|---|---|---|---|---|
| Q7SIG2 |
Chymotrypsin-1
|
EC
3.4.21.1
Chymotrypsin I Soli C1 |
None |
PF00089
(Trypsin)
[Graphical View] |
|
| P00766 |
Chymotrypsinogen A
|
EC
3.4.21.1
|
Chymotrypsin A chain A
Chymotrypsin A chain B Chymotrypsin A chain C |
PF00089
(Trypsin)
[Graphical View] |
S01.001
(Serine)
|
| P00767 |
Chymotrypsinogen B
|
EC
3.4.21.1
|
Chymotrypsin B chain A
Chymotrypsin B chain B Chymotrypsin B chain C |
PF00089
(Trypsin)
[Graphical View] |
S01.152
(Serine)
|
| KEGG enzyme name |
|---|
|
chymotrypsin
chymotrypsins A and B alpha-chymar ophth avazyme chymar chymotest enzeon quimar quimotrase alpha-chymar alpha-chymotrypsin A alpha-chymotrypsin |
| UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
|---|---|---|---|---|---|
| Q7SIG2 | CTR1_SOLIN | Preferential cleavage: Tyr-|-Xaa, Trp-|-Xaa, Phe-|-Xaa, Leu-|-Xaa. | Secreted, extracellular space. | ||
| P00766 | CTRA_BOVIN | Preferential cleavage: Tyr-|-Xaa, Trp-|-Xaa, Phe-|-Xaa, Leu-|-Xaa. | Secreted, extracellular space. | ||
| P00767 | CTRB_BOVIN | Preferential cleavage: Tyr-|-Xaa, Trp-|-Xaa, Phe-|-Xaa, Leu-|-Xaa. | Secreted, extracellular space. |
| KEGG Pathways | Map code | Pathways | E.C. |
|---|
| Compound table | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Substrates | Products | Intermediates | |||||||||
| KEGG-id | C00012 | C00241 | C00001 | C00012 | C04245 | ||||||
| E.C. | |||||||||||
| Compound | Peptide | Amide | H2O | Peptide | Amino acid(Tyr-, Trp-, Phe-, Leu-) | ||||||
| Type | peptide/protein | amide group | H2O | peptide/protein | amino acids | ||||||
| ChEBI |
15377 15377 |
2645 2645 |
|||||||||
| PubChem |
22247451 962 22247451 962 |
440270 440270 |
|||||||||
| 1eq9A01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:PMS | |
| 1eq9B01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:PMS | |
| 1ab9C |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Intermediate-bound:TYR_304(chain D) | |
| 1acbE01 |
|
|
|
|
|
Bound:LEU_45-ASP_46(chain I) | Unbound | Unbound | Unbound | Unbound | |
| 1afqC |
|
|
|
|
|
Analogue:DLE-PHE-FBA(chain D) | Unbound | Unbound | Unbound | Unbound | |
| 1ca0C |
|
|
|
|
|
Analogue:ARG_15-ALA_16(chain D) | Unbound | Unbound | Unbound | Unbound | |
| 1ca0H |
|
|
|
|
|
Analogue:ARG_15-ALA_16(chain I) | Unbound | Unbound | Unbound | Unbound | |
| 1cbwC |
|
|
|
|
|
Analogue:LYS_15-ALA_16(chain D) | Unbound | Unbound | Unbound | Unbound | |
| 1cbwH |
|
|
|
|
|
Analogue:LYS_15-ALA_16(chain I) | Unbound | Unbound | Unbound | Unbound | |
| 1cgiE01 |
|
|
|
|
|
Bound:TYR_18-GLU_19(chain I) | Unbound | Unbound | Unbound | Unbound | |
| 1cgjE01 |
|
|
|
|
|
Bound:LEU_18-GLU_19(chain I) | Unbound | Unbound | Unbound | Unbound | |
| 1chgA01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1choG |
|
|
|
|
|
Bound:LEU_18-GLU_19(chain I) | Unbound | Unbound | Unbound | Unbound | |
| 1dlkB01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:GLY-GLY-PCS(chain I) | |
| 1dlkD01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:GLY-GLY-PCS(chain J) | |
| 1ex3A01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1gcdA01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:DEP | |
| 1gctA01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Intermediate-bound:UNK-PRO-GLY-ALA-TYR(chain B) | |
| 1gg6C |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:APF | |
| 1ggdC |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Intermediate-bound:FAF | |
| 1ghaG |
|
|
|
|
|
Unbound | Unbound | Bound:PRO-GLY-VAL-TYR(chain P) | Unbound | Unbound | |
| 1ghbE01 |
|
|
|
|
|
Unbound | Unbound | Analogue:PRO-GLY-ALA(chain P) | Unbound | Unbound | |
| 1gmcG |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Intermediate-bound:PRO-GLY-ALA-TYR(chain B) | |
| 1gmdG |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Transition-state-bound:PRO-GLY-ALA-TYR-ASP(chain B) | |
| 1gmhG |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:ISP | |
| 1hjaC |
|
|
|
|
|
Analogue:LYS_18-GLU_19(chain I) | Unbound | Unbound | Unbound | Unbound | |
| 1mtnC |
|
|
|
|
|
Analogue:LYS_315-ALA_316(chain D) | Unbound | Unbound | Unbound | Unbound | |
| 1mtnG |
|
|
|
|
|
Analogue:LYS_315-ALA_316(chain H) | Unbound | Unbound | Unbound | Unbound | |
| 1vgcC |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:V36 | |
| 2cgaA01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2cgaB01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2chaC |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:TOS | |
| 2chaG |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:TOS | |
| 2gchG |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2gctA01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Intermediate-bound:UNK-PRO-GLY-ALA-TYR(chain B) | |
| 2gmtA01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2vgcC |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Intermediate-analogue:V35 | |
| 3gchA01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Intermediate-analogue:OAC | |
| 3gctG |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Intermediate-bound:UNK-PRO-GLY-ALA-TYR(chain B) | |
| 3vgcC |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:SRB | |
| 4chaC |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 4chaG |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 4gchG |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Intermediate-analogue:DMC | |
| 4vgcC |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Intermediate-analogue:SRD | |
| 5chaC |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 5chaG |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 5gchG |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 6chaC |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:PBA | |
| 6chaG |
|
|
|
|
|
Unbound | Unbound | Unbound | Analogue:PBA | Unbound | |
| 6gchG |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:APF | |
| 7gchG |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:LPF | |
| 8gchG |
|
|
|
|
|
Unbound | Unbound | Bound:GLY-ALA-TRP(chain C) | Unbound | Unbound | |
| 1eq9A02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1eq9B02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ab9B |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1acbE02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1afqB |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ca0B |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ca0G |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1cbwB |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1cbwG |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1cgiE02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1cgjE02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1chgA02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1choF |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1dlkB02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1dlkD02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ex3A02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1gcdA02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1gctA02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1gg6B |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ggdB |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ghaF |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ghbE02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1gmcF |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1gmdF |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1gmhF |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1hjaB |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1mtnB |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1mtnF |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1vgcB |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2cgaA02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2cgaB02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2chaB |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2chaF |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2gchF |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2gctA02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2gmtA02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2vgcB |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 3gchA02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 3gctF |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 3vgcB |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 4chaB |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 4chaF |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 4gchF |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 4vgcB |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 5chaB |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 5chaF |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 5gchF |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 6chaB |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 6chaF |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 6gchF |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 7gchF |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 8gchF |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| Reference for Active-site residues | ||
|---|---|---|
| resource | references | E.C. |
| Active-site residues | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| PDB | Catalytic residues | Cofactor-binding residues | Modified residues | Main-chain involved in catalysis | Comment | |||||
| 1eq9A01 |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 1eq9B01 |
|
|
|
|
|
SER 495 | GLY 493;SER 495 | |||
| 1ab9C |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 1acbE01 |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 1afqC |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 1ca0C |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 1ca0H |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 1cbwC |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 1cbwH |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 1cgiE01 |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 1cgjE01 |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 1chgA01 |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 1choG |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 1dlkB01 |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 1dlkD01 |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 1ex3A01 |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 1gcdA01 |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 1gctA01 |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 1gg6C |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 1ggdC |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 1ghaG |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 1ghbE01 |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 1gmcG |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 1gmdG |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 1gmhG |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 1hjaC |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 1mtnC |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 1mtnG |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 1vgcC |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 2cgaA01 |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 2cgaB01 |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 2chaC |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 2chaG |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 2gchG |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 2gctA01 |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 2gmtA01 |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 2vgcC |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 3gchA01 |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 3gctG |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 3vgcC |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 4chaC |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 4chaG |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 4gchG |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 4vgcC |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 5chaC |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 5chaG |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 5gchG |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 6chaC |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 6chaG |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 6gchG |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 7gchG |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 8gchG |
|
|
|
|
|
SER 195 | GLY 193;SER 195 | |||
| 1eq9A02 |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 1eq9B02 |
|
|
|
|
|
HIS 357;ASP 402 | ||||
| 1ab9B |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 1acbE02 |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 1afqB |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 1ca0B |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 1ca0G |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 1cbwB |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 1cbwG |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 1cgiE02 |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 1cgjE02 |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 1chgA02 |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 1choF |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 1dlkB02 |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 1dlkD02 |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 1ex3A02 |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 1gcdA02 |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 1gctA02 |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 1gg6B |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 1ggdB |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 1ghaF |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 1ghbE02 |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 1gmcF |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 1gmdF |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 1gmhF |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 1hjaB |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 1mtnB |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 1mtnF |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 1vgcB |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 2cgaA02 |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 2cgaB02 |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 2chaB |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 2chaF |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 2gchF |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 2gctA02 |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 2gmtA02 |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 2vgcB |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 3gchA02 |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 3gctF |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 3vgcB |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 4chaB |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 4chaF |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 4gchF |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 4vgcB |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 5chaB |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 5chaF |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 5gchF |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 6chaB |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 6chaF |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 6gchF |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 7gchF |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| 8gchF |
|
|
|
|
|
HIS 57;ASP 102 | ||||
| References for Catalytic Mechanism | ||
|---|---|---|
| References | Sections | No. of steps in catalysis |
|
[3]
|
Fig.6, p.72-74 | |
|
[4]
|
Fig.4, p.65-67 | |
|
[6]
|
p.299-300 | |
|
[8]
|
Fig.2, p.438-441 | |
|
[13]
|
p.709-710 | |
|
[15]
|
||
|
[17]
|
p.7036-7037 | |
|
[18]
|
p.7606-7607 | |
|
[25]
|
Scheme 1 | |
|
[55]
|
Scheme 1 | |
| References | |
|---|---|
| [1] | |
| Resource | |
| Comments | ACTIVE SITE. |
| Medline ID | 67181723 |
| PubMed ID | 5971785 |
| Journal | Biochem J |
| Year | 1966 |
| Volume | 101 |
| Pages | 232-41 |
| Authors | Smillie LB, Hartley BS |
| Title | Histidine sequences in the active centres of some 'serine' proteinases. |
| Related PDB | |
| Related UniProtKB | P00766 |
| [2] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF CHYMOTRYPSINOGEN. |
| Medline ID | 70177557 |
| PubMed ID | 5442169 |
| Journal | Biochemistry |
| Year | 1970 |
| Volume | 9 |
| Pages | 1997-2009 |
| Authors | Freer ST, Kraut J, Robertus JD, Wright HT, Xuong NH |
| Title |
Chymotrypsinogen: 2.5-angstrom crystal structure, |
| Related PDB | 1chg |
| Related UniProtKB | P00766 |
| [3] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY. |
| Medline ID | 72035052 |
| PubMed ID | 4399050 |
| Journal | Philos Trans R Soc Lond B Biol Sci |
| Year | 1970 |
| Volume | 257 |
| Pages | 67-76 |
| Authors | Birktoft JJ, Blow DM, Henderson R, Steitz TA |
| Title |
I. |
| Related PDB | |
| Related UniProtKB | P00766 |
| [4] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 4508173 |
| Journal | Cold Spring Harb Symp Quant Biol |
| Year | 1972 |
| Volume | 36 |
| Pages | 63-70 |
| Authors | Henderson R, Wright CS, Hess GP, Blow DM |
| Title | Chymotrypsin: what can we learn about catalysis from xray diffraction? |
| Related PDB | |
| Related UniProtKB | |
| [5] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 5069789 |
| Journal | J Mol Biol |
| Year | 1972 |
| Volume | 68 |
| Pages | 187-240 |
| Authors | Birktoft JJ, Blow DM |
| Title |
Structure of crystalline -chymotrypsin. |
| Related PDB | 2cha |
| Related UniProtKB | |
| [6] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 4634509 |
| Journal | J Mol Biol |
| Year | 1972 |
| Volume | 63 |
| Pages | 295-303 |
| Authors | Wright CS, Hess GP, Blow DM |
| Title | Structure of crystalline methylchymotrypsin. |
| Related PDB | |
| Related UniProtKB | |
| [7] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 4745847 |
| Journal | J Mol Biol |
| Year | 1973 |
| Volume | 79 |
| Pages | 1-11 |
| Authors | Wright HT |
| Title | Comparison of the crystal structures of chymotrypsinogenA and alphachymotrypsin. |
| Related PDB | |
| Related UniProtKB | |
| [8] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 4597310 |
| Journal | Ann N Y Acad Sci |
| Year | 1974 |
| Volume | 227 |
| Pages | 438-45 |
| Authors | Hartley BS |
| Title | The active centers of serine proteinases.: Review |
| Related PDB | |
| Related UniProtKB | |
| [9] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF GAMMA-CHYMOTRYPSIN. |
| Medline ID | 82078042 |
| PubMed ID | 6914398 |
| Journal | J Mol Biol |
| Year | 1981 |
| Volume | 148 |
| Pages | 449-79 |
| Authors | Cohen GH, Silverton EW, Davies DR |
| Title |
Refined crystal structure of gamma-chymotrypsin at 1.9 A resolution. |
| Related PDB | 2gch |
| Related UniProtKB | P00766 |
| [10] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 6750612 |
| Journal | Proc Natl Acad Sci U S A |
| Year | 1982 |
| Volume | 79 |
| Pages | 4868-72 |
| Authors | Fujinaga M, Read RJ, Sielecki A, Ardelt W, Laskowski M Jr, James MN |
| Title |
Refined crystal structure of the molecular complex of Streptomyces griseus protease B, |
| Related PDB | |
| Related UniProtKB | |
| [11] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 3971987 |
| Journal | Eur J Biochem |
| Year | 1985 |
| Volume | 147 |
| Pages | 387-95 |
| Authors | Bode W, Epp O, Huber R, Laskowski M Jr, Ardelt W |
| Title | The crystal and molecular structure of the third domain of silver pheasant ovomucoid (OMSVP3). |
| Related PDB | |
| Related UniProtKB | |
| [12] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 3980476 |
| Journal | J Biol Chem |
| Year | 1985 |
| Volume | 260 |
| Pages | 4264-75 |
| Authors | Blevins RA, Tulinsky A |
| Title | The refinement and the structure of the dimer of alpha-chymotrypsin at 1.67-A resolution. |
| Related PDB | 5cha |
| Related UniProtKB | |
| [13] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (1.68 ANGSTROMS) OF ALPHA-CHYMOTRYPSIN. |
| Medline ID | 86011575 |
| PubMed ID | 4046030 |
| Journal | J Mol Biol |
| Year | 1985 |
| Volume | 184 |
| Pages | 703-11 |
| Authors | Tsukada H, Blow DM |
| Title | Structure of alpha-chymotrypsin refined at 1.68 A resolution. |
| Related PDB | 4cha |
| Related UniProtKB | P00766 |
| [14] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 4057257 |
| Journal | J Mol Biol |
| Year | 1985 |
| Volume | 185 |
| Pages | 595-624 |
| Authors | Wang D, Bode W, Huber R |
| Title | Bovine chymotrypsinogen A X-ray crystal structure analysis and refinement of a new crystal form at 1.8 A resolution. |
| Related PDB | 2cga |
| Related UniProtKB | |
| [15] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 3584139 |
| Journal | J Biol Chem |
| Year | 1987 |
| Volume | 262 |
| Pages | 7737-43 |
| Authors | Tulinsky A, Blevins RA |
| Title | Structure of a tetrahedral transition state complex of alpha-chymotrypsin dimer at 1.8-A resolution. |
| Related PDB | 6cha |
| Related UniProtKB | |
| [16] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 3477645 |
| Journal | J Mol Biol |
| Year | 1987 |
| Volume | 195 |
| Pages | 397-418 |
| Authors | Fujinaga M, Sielecki AR, Read RJ, Ardelt W, Laskowski M Jr, James MN |
| Title | Crystal and molecular structures of the complex of alpha-chymotrypsin with its inhibitor turkey ovomucoid third domain at 1.8 A resolution. |
| Related PDB | 1cho |
| Related UniProtKB | |
| [17] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 2819046 |
| Journal | Biochemistry |
| Year | 1989 |
| Volume | 28 |
| Pages | 7033-8 |
| Authors | Dixon MM, Matthews BW |
| Title | Is gamma-chymotrypsin a tetrapeptide acyl-enzyme adduct of alpha-chymotrypsin? |
| Related PDB | 1gct |
| Related UniProtKB | |
| [18] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 2271520 |
| Journal | Biochemistry |
| Year | 1990 |
| Volume | 29 |
| Pages | 7600-7 |
| Authors | Brady K, Wei AZ, Ringe D, Abeles RH |
| Title | Structure of chymotrypsin-trifluoromethyl ketone inhibitor complexes: comparison of slowly and rapidly equilibrating inhibitors. |
| Related PDB | 6gch 7gch |
| Related UniProtKB | |
| [19] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 2364065 |
| Journal | Biochemistry |
| Year | 1990 |
| Volume | 29 |
| Pages | 4871-9 |
| Authors | Stoddard BL, Bruhnke J, Porter N, Ringe D, Petsko GA |
| Title | Structure and activity of two photoreversible cinnamates bound to chymotrypsin. |
| Related PDB | 4gch |
| Related UniProtKB | |
| [20] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 2036388 |
| Journal | Biochemistry |
| Year | 1991 |
| Volume | 30 |
| Pages | 5217-25 |
| Authors | Harel M, Su CT, Frolow F, Silman I, Sussman JL |
| Title | Gamma-chymotrypsin is a complex of alpha-chymotrypsin with its own autolysis products. |
| Related PDB | 8gch |
| Related UniProtKB | |
| [21] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 1888717 |
| Journal | Int J Biol Macromol |
| Year | 1991 |
| Volume | 13 |
| Pages | 89-96 |
| Authors | Dixon MM, Brennan RG, Matthews BW |
| Title | Structure of gamma-chymotrypsin in the range pH 2.0 to pH 10.5 suggests that gamma-chymotrypsin is a covalent acyl-enzyme adduct at low pH. |
| Related PDB | 2gct 3gct |
| Related UniProtKB | |
| [22] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 1942036 |
| Journal | J Mol Biol |
| Year | 1991 |
| Volume | 221 |
| Pages | 909-18 |
| Authors | Harel M, Su CT, Frolow F, Ashani Y, Silman I, Sussman JL |
| Title | Refined crystal structures of "aged" and "non-aged" organophosphoryl conjugates of gamma-chymotrypsin. |
| Related PDB | 1gcd 1gmh |
| Related UniProtKB | |
| [23] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 1870127 |
| Journal | J Mol Biol |
| Year | 1991 |
| Volume | 220 |
| Pages | 711-22 |
| Authors | Hecht HJ, Szardenings M, Collins J, Schomburg D |
| Title | Three-dimensional structure of the complexes between bovine chymotrypsinogen A and two recombinant variants of human pancreatic secretory trypsin inhibitor (Kazal-type). |
| Related PDB | 1cgi 1cgj |
| Related UniProtKB | |
| [24] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 1583684 |
| Journal | J Mol Biol |
| Year | 1992 |
| Volume | 225 |
| Pages | 107-23 |
| Authors | Frigerio F, Coda A, Pugliese L, Lionetti C, Menegatti E, Amiconi G, Schnebli HP, Ascenzi P, Bolognesi M |
| Title | Crystal and molecular structure of the bovine alpha-chymotrypsin-eglin c complex at 2.0 A resolution. |
| Related PDB | 1acb |
| Related UniProtKB | |
| [25] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 7947790 |
| Journal | Biochemistry |
| Year | 1994 |
| Volume | 33 |
| Pages | 13792-800 |
| Authors | Kreutter K, Steinmetz AC, Liang TC, Prorok M, Abeles RH, Ringe D |
| Title | Three-dimensional structure of chymotrypsin inactivated with (2S)-N-acetyl-L-alanyl-L-phenylalanyl alpha-chloroethane: implications for the mechanism of inactivation of serine proteases by chloroketones. |
| Related PDB | 2gmt |
| Related UniProtKB | |
| [26] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 8003497 |
| Journal | Biochemistry |
| Year | 1994 |
| Volume | 33 |
| Pages | 7326-36 |
| Authors | Yennawar NH, Yennawar HP, Farber GK |
| Title | X-ray crystal structure of gamma-chymotrypsin in hexane. |
| Related PDB | 1ab9 1afq 1gmc 1gmd |
| Related UniProtKB | |
| [27] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8089842 |
| Journal | J Mol Biol |
| Year | 1994 |
| Volume | 242 |
| Pages | 203-14 |
| Authors | Krezel AM, Darba P, Robertson AD, Fejzo J, Macura S, Markley JL |
| Title | Solution structure of turkey ovomucoid third domain as determined from nuclear magnetic resonance data. |
| Related PDB | |
| Related UniProtKB | |
| [28] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | |
| Journal | J Am Chem Soc |
| Year | 1995 |
| Volume | 117 |
| Pages | 577-85 |
| Authors | Yennawar HP, Yennawar NH, Farber GK |
| Title | A structural explanation for enzyme memory in nonaqueous solvents. |
| Related PDB | 1gha 1ghb |
| Related UniProtKB | |
| [29] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8642605 |
| Journal | J Mol Biol |
| Year | 1996 |
| Volume | 258 |
| Pages | 501-37 |
| Authors | Lesk AM, Fordham WD |
| Title | Conservation and variability in the structures of serine proteinases of the chymotrypsin family. |
| Related PDB | |
| Related UniProtKB | |
| [30] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 9179777 |
| Journal | J Mol Recognit |
| Year | 1997 |
| Volume | 10 |
| Pages | 26-35 |
| Authors | Capasso C, Rizzi M, Menegatti E, Ascenzi P, Bolognesi M |
| Title |
Crystal structure of the bovine alpha-chymotrypsin:Kunitz inhibitor complex. |
| Related PDB | 1mtn |
| Related UniProtKB | |
| [31] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 9300481 |
| Journal | Protein Sci |
| Year | 1997 |
| Volume | 6 |
| Pages | 1806-24 |
| Authors | Scheidig AJ, Hynes TR, Pelletier LA, Wells JA, Kossiakoff AA |
| Title | Crystal structures of bovine chymotrypsin and trypsin complexed to the inhibitor domain of Alzheimer's amyloid beta-protein precursor (APPI) and basic pancreatic trypsin inhibitor (BPTI): engineering of inhibitors with altered specificities. |
| Related PDB | 1ca0 1cbw |
| Related UniProtKB | |
| [32] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 9425066 |
| Journal | Biochemistry |
| Year | 1998 |
| Volume | 37 |
| Pages | 451-62 |
| Authors | Stoll VS, Eger BT, Hynes RC, Martichonok V, Jones JB, Pai EF |
| Title | Differences in binding modes of enantiomers of 1-acetamido boronic acid based protease inhibitors: crystal structures of gamma-chymotrypsin and subtilisin Carlsberg complexes. |
| Related PDB | 1vgc 2vgc 3vgc 4vgc |
| Related UniProtKB | |
| [33] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 10713514 |
| Journal | Acta Crystallogr D Biol Crystallogr |
| Year | 2000 |
| Volume | 56 |
| Pages | 280-6 |
| Authors | Mac Sweeney A, Birrane G, Walsh MA, O'Connell T, Malthouse JP, Higgins TM |
| Title | Crystal structure of delta-chymotrypsin bound to a peptidyl chloromethyl ketone inhibitor. |
| Related PDB | 1dlk |
| Related UniProtKB | |
| [34] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 10801356 |
| Journal | J Mol Biol |
| Year | 2000 |
| Volume | 298 |
| Pages | 895-901 |
| Authors | Botos I, Meyer E, Nguyen M, Swanson SM, Koomen JM, Russell DH, Meyer EF |
| Title | The structure of an insect chymotrypsin. |
| Related PDB | 1eq9 |
| Related UniProtKB | |
| [35] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 10873462 |
| Journal | J Mol Biol |
| Year | 2000 |
| Volume | 300 |
| Pages | 235-9 |
| Authors | Pjura PE, Lenhoff AM, Leonard SA, Gittis AG |
| Title | Protein crystallization by design: chymotrypsinogen without precipitants. |
| Related PDB | 1ex3 |
| Related UniProtKB | |
| [36] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11327865 |
| Journal | Biochemistry |
| Year | 2001 |
| Volume | 40 |
| Pages | 2439-47 |
| Authors | Neidhart D, Wei Y, Cassidy C, Lin J, Cleland WW, Frey PA |
| Title | Correlation of low-barrier hydrogen bonding and oxyanion binding in transition state analogue complexes of chymotrypsin. |
| Related PDB | 1gg6 1ggd |
| Related UniProtKB | |
| [37] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11258883 |
| Journal | Biochemistry |
| Year | 2001 |
| Volume | 40 |
| Pages | 2723-31 |
| Authors | Pan Y, Daggett V |
| Title | Direct comparison of experimental and calculated folding free energies for hydrophobic deletion mutants of chymotrypsin inhibitor 2: free energy perturbation calculations using transition and denatured states from molecular dynamics simulations of unfolding. |
| Related PDB | |
| Related UniProtKB | |
| [38] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11733020 |
| Journal | Eur J Biochem |
| Year | 2001 |
| Volume | 268 |
| Pages | 6238-46 |
| Authors | Bodi A, Kaslik G, Venekei I, Graf L |
| Title | Structural determinants of the half-life and cleavage site preference in the autolytic inactivation of chymotrypsin. |
| Related PDB | |
| Related UniProtKB | |
| [39] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11562364 |
| Journal | J Biol Chem |
| Year | 2001 |
| Volume | 276 |
| Pages | 45079-87 |
| Authors | Chen C, Hsu CH, Su NY, Lin YC, Chiou SH, Wu SH |
| Title | Solution structure of a Kunitz-type chymotrypsin inhibitor isolated from the elapid snake Bungarus fasciatus. |
| Related PDB | |
| Related UniProtKB | |
| [40] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11495915 |
| Journal | J Biol Chem |
| Year | 2001 |
| Volume | 276 |
| Pages | 38893-8 |
| Authors | Roussel A, Mathieu M, Dobbs A, Luu B, Cambillau C, Kellenberger C |
| Title | Complexation of two proteic insect inhibitors to the active site of chymotrypsin suggests decoupled roles for binding and selectivity. |
| Related PDB | |
| Related UniProtKB | |
| [41] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11846564 |
| Journal | J Mol Biol |
| Year | 2001 |
| Volume | 314 |
| Pages | 519-25 |
| Authors | Ghani U, Ng KK, Atta-ur-Rahman, Choudhary MI, Ullah N, James MN |
| Title | Crystal structure of gamma-chymotrypsin in complex with 7-hydroxycoumarin. |
| Related PDB | |
| Related UniProtKB | |
| [42] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11391787 |
| Journal | J Mol Recognit |
| Year | 2001 |
| Volume | 14 |
| Pages | 166-71 |
| Authors | Song J, Markley JL |
| Title |
NMR chemical shift mapping of the binding site of a protein proteinase inhibitor: changes in the (1)H, |
| Related PDB | |
| Related UniProtKB | |
| [43] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12054616 |
| Journal | Biochem Biophys Res Commun |
| Year | 2002 |
| Volume | 293 |
| Pages | 416-20 |
| Authors | Simon LM, Kotorman M, Garab G, Laczko I |
| Title | Effects of polyhydroxy compounds on the structure and activity of alpha-chymotrypsin. |
| Related PDB | |
| Related UniProtKB | |
| [44] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11909728 |
| Journal | Bioorg Med Chem Lett |
| Year | 2002 |
| Volume | 12 |
| Pages | 1109-12 |
| Authors | Wouters J, Huygens M, Pochet L, Pirotte B, Durant F, Masereel B |
| Title | Structural approach of the mechanism of inhibition of alpha-chymotrypsin by coumarins. |
| Related PDB | |
| Related UniProtKB | |
| [45] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12209825 |
| Journal | Biotechnol Bioeng |
| Year | 2002 |
| Volume | 79 |
| Pages | 539-49 |
| Authors | Clark DS, Bailey JE |
| Title | Structure-function relationships in immobilized chymotrypsin catalysis. |
| Related PDB | |
| Related UniProtKB | |
| [46] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11960433 |
| Journal | J Am Chem Soc |
| Year | 2002 |
| Volume | 124 |
| Pages | 4196-7 |
| Authors | Westler WM, Frey PA, Lin J, Wemmer DE, Morimoto H, Williams PG, Markley JL |
| Title | Evidence for a strong hydrogen bond in the catalytic dyad of transition-state analogue inhibitor complexes of chymotrypsin from proton-triton NMR isotope shifts. |
| Related PDB | |
| Related UniProtKB | |
| [47] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12452711 |
| Journal | J Am Chem Soc |
| Year | 2002 |
| Volume | 124 |
| Pages | 14373-81 |
| Authors | Westler WM, Weinhold F, Markley JL |
| Title | Quantum chemical calculations on structural models of the catalytic site of chymotrypsin: comparison of calculated results with experimental data from NMR spectroscopy. |
| Related PDB | |
| Related UniProtKB | |
| [48] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12015318 |
| Journal | J Biol Chem |
| Year | 2002 |
| Volume | 277 |
| Pages | 28031-7 |
| Authors | Kolodziej SJ, Wagenknecht T, Strickland DK, Stoops JK |
| Title | The three-dimensional structure of the human alpha 2-macroglobulin dimer reveals its structural organization in the tetrameric native and chymotrypsin alpha 2-macroglobulin complexes. |
| Related PDB | |
| Related UniProtKB | |
| [49] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12427971 |
| Journal | Proc Natl Acad Sci U S A |
| Year | 2002 |
| Volume | 99 |
| Pages | 15297-302 |
| Authors | Pal SK, Peon J, Zewail AH |
| Title | Ultrafast surface hydration dynamics and expression of protein functionality: alpha -Chymotrypsin. |
| Related PDB | |
| Related UniProtKB | |
| [50] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12705877 |
| Journal | Biochem Biophys Res Commun |
| Year | 2003 |
| Volume | 304 |
| Pages | 18-21 |
| Authors | Kotorman M, Laczko I, Szabo A, Simon LM |
| Title | Effects of Ca2+ on catalytic activity and conformation of trypsin and alpha-chymotrypsin in aqueous ethanol. |
| Related PDB | |
| Related UniProtKB | |
| [51] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12589768 |
| Journal | J Mol Biol |
| Year | 2003 |
| Volume | 326 |
| Pages | 1271-88 |
| Authors | Filfil R, Chalikian TV |
| Title | The thermodynamics of protein-protein recognition as characterized by a combination of volumetric and calorimetric techniques: the binding of turkey ovomucoid third domain to alpha-chymotrypsin. |
| Related PDB | |
| Related UniProtKB | |
| [52] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 14568540 |
| Journal | J Mol Biol |
| Year | 2003 |
| Volume | 333 |
| Pages | 845-61 |
| Authors | Helland R, Czapinska H, Leiros I, Olufsen M, Otlewski J, Smalas AO |
| Title | Structural consequences of accommodation of four non-cognate amino acid residues in the S1 pocket of bovine trypsin and chymotrypsin. |
| Related PDB | |
| Related UniProtKB | |
| [53] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12927546 |
| Journal | J Mol Biol |
| Year | 2003 |
| Volume | 331 |
| Pages | 1121-30 |
| Authors | Szabo E, Venekei I, Bocskei Z, Naray-Szabo G, Graf L |
| Title | Three dimensional structures of S189D chymotrypsin and D189S trypsin mutants: the effect of polarity at site 189 on a protease-specific stabilization of the substrate-binding site. |
| Related PDB | |
| Related UniProtKB | |
| [54] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 14560053 |
| Journal | Protein Eng |
| Year | 2003 |
| Volume | 16 |
| Pages | 673-81 |
| Authors | Mucsi Z, Gaspari Z, Orosz G, Perczel A |
| Title | Structure-oriented rational design of chymotrypsin inhibitor models. |
| Related PDB | |
| Related UniProtKB | |
| [55] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 14730979 |
| Journal | Biochemistry |
| Year | 2004 |
| Volume | 43 |
| Pages | 742-7 |
| Authors | Hengge AC, Stein RL |
| Title | Role of protein conformational mobility in enzyme catalysis: acylation of alpha-chymotrypsin by specific peptide substrates. |
| Related PDB | |
| Related UniProtKB | |
| Comments |
|---|
|
This enzyme belongs to the peptidase family-S1.
This enzyme is one of calssical serine proteases. According to the literature, (1) His57 acts as a general base, (2) Ser195, (3) The oxyanion of the transition-state is stabilized by the mainchain amide nitrogens. (4) His57 acts as a general acid, (5) A water molecule makes a nucleophilic attack on the acyl group, |
| Created | Updated |
|---|---|
| 2004-10-14 | 2009-03-30 |