DB code: M00181

RLCP classification 1.13.30000.10 : Hydrolysis
CATH domain 2.40.10.10 : Thrombin, subunit H Catalytic domain
2.40.10.10 : Thrombin, subunit H Catalytic domain
2.40.10.10 : Thrombin, subunit H
2.40.10.10 : Thrombin, subunit H
2.10.90.10 : Cystine Knot Cytokines, subunit B
E.C. 3.4.21.35
CSA
M-CSA
MACiE

CATH domain Related DB codes (homologues)
2.40.10.10 : Thrombin, subunit H M00139 D00214 M00167 D00426 M00133 D00428 D00429 D00430 D00431 D00432 D00433 D00434 D00435 M00227 M00209 D00194 D00197 D00211 D00212 D00216 M00212 D00224 D00497 M00217 M00216 D00528 D00848 D00850 D00851 D00852 D00855 M00152 M00155 M00157 M00315 M00316 M00317 M00348 M00349 T00074 T00410 T00411

Uniprot Enzyme Name
UniprotKB Protein name Synonyms Contains RefSeq MEROPS Pfam
P00756 Kallikrein 1-related peptidase b3
EC 3.4.21.35
Glandular kallikrein K3
mGK-3
Tissue kallikrein-3
7S nerve growth factor gamma chain
Gamma-NGF
Nerve growth factor gamma chain 1
Nerve growth factor gamma chain 2
NP_032719.1 (Protein)
NM_008693.2 (DNA/RNA sequence)
S01.170 (Serine)
PF00089 (Trypsin)
[Graphical View]
P00757 Kallikrein 1-related peptidase-like b4
7S nerve growth factor alpha chain
Alpha-NGF
None NP_035045.2 (Protein)
NM_010915.3 (DNA/RNA sequence)
S01.931 (Serine)
PF00089 (Trypsin)
[Graphical View]
P01139 Beta-nerve growth factor
Beta-NGF
None NP_001106168.1 (Protein)
NM_001112698.1 (DNA/RNA sequence)
NP_038637.1 (Protein)
NM_013609.2 (DNA/RNA sequence)
PF00243 (NGF)
[Graphical View]

KEGG enzyme name
tissue kallikrein
glandular kallikrein
pancreatic kallikrein
submandibular kallikrein
submaxillary kallikrein
kidney kallikrein
urinary kallikrein
kallikrein
salivary kallikrein
kininogenin
kininogenase
callicrein
glumorin
padreatin
padutin
kallidinogenase
bradykininogenase
depot-padutin
urokallikrein
dilminal D
onokrein P

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P00756 K1KB3_MOUSE Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa. 7S nerve growth factor is composed of two alpha chains, a beta dimer composed of identical chains, and two gamma chains. Binds 2 zinc ions per 7S complex. The zinc ions are bound at the alpha-gamma interfaces.
P00757 K1KB4_MOUSE 7S nerve growth factor is composed of two alpha chains, a beta dimer composed of identical chains, and two gamma chains. Binds 2 zinc ions per 7S complex. The zinc ions are bound at the alpha-gamma interfaces.
P01139 NGF_MOUSE Homodimer. Secreted.

KEGG Pathways
Map code Pathways E.C.

Compound table
Cofactors Substrates Products Intermediates
KEGG-id C00038 C00017 C00012 C00769 C00001 C00012 C01505 C00306 I00087 I00085 I00086
E.C.
Compound Zinc Protein Peptide Kininogen H2O Peptide Kallidin Bradykinin Peptidyl-tetrahedral intermediate Acyl-enzyme Tetrahedral intermediate
Type heavy metal peptide/protein peptide/protein peptide/protein H2O peptide/protein amino acids,amine group,aromatic ring (only carbon atom),carbohydrate,carboxyl group,imine group,lipid,peptide/protein amino acids,amine group,aromatic ring (only carbon atom),carbohydrate,carboxyl group,imine group,lipid,peptide/protein
ChEBI 29105
 29105
 		15377
 15377
 		6102
 6102
 		3165
 3165
PubChem 32051
 32051
 		22247451
 962
 22247451
 962
 		5311111
 5311111
 		439201
 439201
1sgfG01 Bound:_ZN Unbound Unbound Unbound Unbound Unbound Unbound
1sgfZ01 Bound:_ZN Unbound Unbound Unbound Unbound Unbound Unbound
1sgfG02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1sgfZ02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1sgfA01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1sgfX01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1sgfA02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1sgfX02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1sgfB Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1sgfY Unbound Unbound Unbound Unbound Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1sgfG01 SER 195 HIS 217;GLU 222(Zinc binding) GLY 193;SER 195
1sgfZ01 SER 195 HIS 217;GLU 222(Zinc binding) GLY 193;SER 195
1sgfG02 HIS 57;ASP 102
1sgfZ02 HIS 57;ASP 102
1sgfA01
1sgfX01
1sgfA02 GLU 75;HIS 82(Zinc binding)
1sgfX02 ;HIS 82(Zinc binding) invisible 71-76, 78-79
1sgfB
1sgfY

References for Catalytic Mechanism
References Sections No. of steps in catalysis

References
[1]
Resource
Comments
Medline ID
PubMed ID 8401208
Journal Protein Sci
Year 1993
Volume 2
Pages 1229-41
Authors Bax B, Blaber M, Ferguson G, Sternberg MJ, Walls PH
Title Prediction of the three-dimensional structures of the nerve growth factor and epidermal growth factor binding proteins (kallikreins) and an hypothetical structure of the high molecular weight complex of epidermal growth factor with its binding protein.
Related PDB
Related UniProtKB
[2]
Resource
Comments X-RAY CRYSTALLOGRAPHY (3.15 ANGSTROMS) OF 7S COMPLEX.
Medline ID
PubMed ID 9351801
Journal Structure
Year 1997
Volume 5
Pages 1275-85
Authors Bax B, Blundell TL, Murray-Rust J, McDonald NQ
Title Structure of mouse 7S NGF: a complex of nerve growth factor with four binding proteins.
Related PDB 1sgf
Related UniProtKB P00756

Comments
This enzyme belongs to the peptidase family-S1.
This enzyme, 7S nerve growth factor, is composed of two alpha chains, a beta dimer composed of identical chains, and two gamma chains. The alpha and gamma chains belong to the peptidase family-S1. However, although the gamma chains have got peptidase activity, the alpha chains do not have activity.
Although this enzyme binds two zinc ions per complex, they are not involved in catalysis, as they bound at the interface of alpha/gamma subunits.
The gamma subunits have got a catalytic triad, composed of Ser/His/Asp and an oxyanionhole of mainchain amide groups. Thus, it must have a similar reaction mechanism to that of trypsin (D00197 in EzCatDB).

Created Updated
2006-01-10 2011-02-18