DB code: D00851

CATH domain	Related DB codes (homologues)
2.40.10.10 : Thrombin, subunit H	M00139 D00214 M00167 D00426 M00133 D00428 D00429 D00430 D00431 D00432 D00433 D00434 D00435 M00227 M00209 D00194 D00197 D00211 D00212 D00216 M00212 D00224 D00497 M00217 M00216 D00528 D00848 D00850 D00852 D00855 M00152 M00155 M00157 M00181 M00315 M00316 M00317 M00348 M00349 T00074 T00410 T00411

Uniprot Enzyme Name
UniprotKB	Protein name	Synonyms	MEROPS	RefSeq
P0C1U8	Glutamyl endopeptidase	EC 3.4.21.19 Endoproteinase Glu-C Staphylococcal serine proteinase V8 protease V8 proteinase	S01.269 (Serine)
Q99V45	Glutamyl endopeptidase	EC 3.4.21.19 Endoproteinase Glu-C Staphylococcal serine proteinase V8 protease V8 proteinase		NP_371572.1 (Protein) NC_002758.2 (DNA/RNA sequence)

KEGG enzyme name
Glutamyl endopeptidase V8 proteinase Endoproteinase Glu-C Staphylococcal serine proteinase

UniprotKB: Accession Number	Entry name	Activity	Subunit	Subcellular location	Cofactor
P0C1U8	SSPA_STAAU	Preferential cleavage: Glu-\|-Xaa, Asp-\|-Xaa.		Secreted.
Q99V45	SSPA_STAAM	Preferential cleavage: Glu-\|-Xaa, Asp-\|-Xaa.		Secreted (By similarity).

KEGG Pathways
Map code	Pathways	E.C.

Compound table
	Substrates		Products	Intermediates
KEGG-id	C00017	C00001	C00017	I00087	I00085	I00086
E.C.
Compound	Protein	H2O	Protein	Peptidyl-Ser-tetrahedral-intermediate (with previous peptide)	Acyl-enzyme(Peptidyl-Ser-acyl group)	Peptidyl-Ser-tetrahedral-intermediate
Type	peptide/protein	H2O	peptide/protein
ChEBI		15377 15377
PubChem		22247451 962 22247451 962

1wczA01	Unbound		Unbound	Unbound	Unbound	Unbound
1qy6A01	Unbound		Unbound	Unbound	Unbound	Unbound
2o8lA01	Unbound		Unbound	Unbound	Unbound	Unbound
1wczA02	Unbound		Unbound	Unbound	Unbound	Unbound
1qy6A02	Unbound		Unbound	Unbound	Unbound	Unbound
2o8lA02	Unbound		Unbound	Unbound	Unbound	Unbound

Reference for Active-site residues
resource	references	E.C.
Literature [4] & Swiss-prot;P0C1U8, Q99V45

Active-site residues
PDB	Catalytic residues	Cofactor-binding residues	Modified residues	Main-chain involved in catalysis	Comment

1wczA01	SER 169			GLY 167;SER 169
1qy6A01	SER 169			GLY 167;SER 169
2o8lA01	SER 169			GLY 167;SER 169
1wczA02	HIS 51;ASP 93
1qy6A02	HIS 51;ASP 93
2o8lA02	HIS 51;ASP 93

References for Catalytic Mechanism
References	Sections	No. of steps in catalysis
[3]	Figure 5, p.4505-4508

References
[1]
Resource
Comments
Medline ID
PubMed ID	4509307
Journal	Proc Natl Acad Sci U S A
Year	1972
Volume	69
Pages	3506-9
Authors	Houmard J, Drapeau GR
Title	Staphylococcal protease: a proteolytic enzyme specific for glutamoyl bonds.
Related PDB
Related UniProtKB
[2]
Resource
Comments
Medline ID
PubMed ID	136253
Journal	Biochem Biophys Res Commun
Year	1976
Volume	72
Pages	411-7
Authors	Austen BM, Smith EL
Title	Action of staphylococcal proteinase on peptides of varying chain length and composition.
Related PDB
Related UniProtKB
[3]
Resource
Comments
Medline ID
PubMed ID	12475199
Journal	Chem Rev
Year	2002
Volume	102
Pages	4501-24
Authors	Hedstrom L
Title	Serine protease mechanism and specificity.
Related PDB
Related UniProtKB
[4]
Resource
Comments	X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 69-342.
Medline ID
PubMed ID	14747701
Journal	Acta Crystallogr D Biol Crystallogr
Year	2004
Volume	60
Pages	256-9
Authors	Prasad L, Leduc Y, Hayakawa K, Delbaere LT
Title	The structure of a universally employed enzyme: V8 protease from Staphylococcus aureus.
Related PDB	1qy6 2o8l
Related UniProtKB	Q99V45
[5]
Resource
Comments
Medline ID
PubMed ID	17878159
Journal	J Biol Chem
Year	2007
Volume	282
Pages	34129-38
Authors	Nickerson NN, Prasad L, Jacob L, Delbaere LT, McGavin MJ
Title	Activation of the SspA serine protease zymogen of Staphylococcus aureus proceeds through unique variations of a trypsinogen-like mechanism and is dependent on both autocatalytic and metalloprotease-specific processing.
Related PDB
Related UniProtKB

Comments
This enzyme belongs to peptidase family-S1B. Since this enzyme has got the same catalytic site as trypsin, it must catalyze the trypsin-like reaction.

Created	Updated
2011-02-24	2012-07-31