DB code: D00528

RLCP classification	1.13.30000.10 : Hydrolysis
CATH domain	2.40.10.10 : Thrombin, subunit H	Catalytic domain
CATH domain	2.40.10.10 : Thrombin, subunit H	Catalytic domain
E.C.	3.4.21.119
CSA
M-CSA
MACiE

CATH domain	Related DB codes (homologues)
2.40.10.10 : Thrombin, subunit H	M00139 D00214 M00167 D00426 M00133 D00428 D00429 D00430 D00431 D00432 D00433 D00434 D00435 M00227 M00209 D00194 D00197 D00211 D00212 D00216 M00212 D00224 D00497 M00217 M00216 D00848 D00850 D00851 D00852 D00855 M00152 M00155 M00157 M00181 M00315 M00316 M00317 M00348 M00349 T00074 T00410 T00411

Uniprot Enzyme Name
UniprotKB	Protein name	Synonyms	RefSeq	Pfam
P36368	Epidermal growth factor-binding protein type B	EGF-BP B EC 3.4.21.119 Glandular kallikrein K13 mGK-13 Tissue kallikrein 13 Prorenin-converting enzyme 1 PRECE-1	NP_034245.3 (Protein) NM_010115.6 (DNA/RNA sequence)	PF00089 (Trypsin) [Graphical View]

KEGG enzyme name
kallikrein 13 KLK13 kallikrein mK13 mGK-13 mK13 mKLK13 prorenin converting enzyme 1 PRECE-1 prorenin-converting enzyme PRECE proteinase P

UniprotKB: Accession Number	Entry name	Activity	Subunit	Subcellular location	Cofactor
P36368	EGFB2_MOUSE	Hydrolyzes mouse Ren2 protein (a species of prorenin present in the submandibular gland) on the carboxy side of the arginine residue at the Lys-Arg-\|- pair in the N-terminus, to yield mature renin.

KEGG Pathways
Map code	Pathways	E.C.

Compound table
	Substrates		Products		Intermediates
KEGG-id	L00034	C00001	L00035	L00036	I00087	I00085	I00086
E.C.
Compound	Prorenin	H2O	Renin	Lys-Arg	Peptidyl-tetrahedral intermediate	Acyl-enzyme	Tetrahedral intermediate
Type	amide group,amine group,imine group,lipid,peptide/protein	H2O	peptide/protein	amide group,amine group,imine group,lipid,peptide/protein
ChEBI		15377 15377		74553 74553
PubChem		22247451 962 22247451 962		15061422 15061422

1ao5A01	Unbound		Unbound	Unbound	Unbound	Unbound	Unbound
1ao5B01	Unbound		Unbound	Unbound	Unbound	Unbound	Unbound
1ao5A02	Unbound		Unbound	Unbound	Unbound	Unbound	Unbound
1ao5B02	Unbound		Unbound	Unbound	Unbound	Unbound	Unbound

Reference for Active-site residues
resource	references	E.C.

Active-site residues
PDB	Catalytic residues	Cofactor-binding residues	Modified residues	Main-chain involved in catalysis	Comment

1ao5A01	SER 195			ASP 193;SER 195
1ao5B01	SER 195			ASP 193;SER 195
1ao5A02	HIS 57;ASP 102
1ao5B02	HIS 57;ASP 102

References for Catalytic Mechanism
References	Sections	No. of steps in catalysis
[9]
[13]	Scheme 3, Scheme 10, Scheme 11, p.94-104
[14]	p.2039
[21]	p.258-259
[29]	p.27277-27278
[30]	p.329-332

References
[1]
Resource
Comments	REVIEW.
Medline ID	82194877
PubMed ID	7043199
Journal	Methods Enzymol
Year	1981
Volume	80 Pt C
Pages	493-532
Authors	Fiedler F, Fink E, Tschesche H, Fritz H
Title	Porcine glandular kallikreins.
Related PDB
Related UniProtKB	P00752
[2]
Resource
Comments	X-RAY CRYSTALLOGRAPHY (2 ANGSTROMS), AND REVISIONS.
Medline ID	83189107
PubMed ID	6551452
Journal	J Mol Biol
Year	1983
Volume	164
Pages	237-82
Authors	Bode W, Chen Z, Bartels K, Kutzbach C, Schmidt-Kastner G, Bartunik H
Title	Refined 2 A X-ray crystal structure of porcine pancreatic kallikrein A, a specific trypsin-like serine proteinase. Crystallization, structure determination, crystallographic refinement, structure and its comparison with bovine trypsin.
Related PDB	2pka
Related UniProtKB	P00752
[3]
Resource
Comments	X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF COMPLEX WITH BOVINE PANCREATIC TRYPSIN INHIBITOR.
Medline ID	83189108
PubMed ID	6188842
Journal	J Mol Biol
Year	1983
Volume	164
Pages	283-311
Authors	Chen Z, Bode W
Title	Refined 2.5 A X-ray crystal structure of the complex formed by porcine kallikrein A and the bovine pancreatic trypsin inhibitor. Crystallization, Patterson search, structure determination, refinement, structure and comparison with its components and with the bovine trypsin-pancreatic trypsin inhibitor complex.
Related PDB	2kai
Related UniProtKB	P00752
[4]
Resource
Comments
Medline ID
PubMed ID	2954262
Journal	Thromb Res
Year	1987
Volume	45
Pages	451-62
Authors	Hijikata-Okunomiya A, Okamoto S, Kikumoto R, Tamao Y, Ohkubo K, Tezuka T, Tonomura S, Matsumoto O
Title	Similarity and dissimilarity in the stereogeometry of the active sites of thrombin, trypsin, plasmin and glandular kallikrein.
Related PDB
Related UniProtKB
[5]
Resource
Comments	STRUCTURE OF CARBOHYDRATES.
Medline ID	89062455
PubMed ID	3196708
Journal	Biochemistry
Year	1988
Volume	27
Pages	7146-54
Authors	Tomiya N, Yamaguchi T, Awaya J, Kurono M, Endo S, Arata Y, Takahashi N, Ishihara H, Mori M, Tejima S
Title	Structural analyses of asparagine-linked oligosaccharides of porcine pancreatic kallikrein.
Related PDB
Related UniProtKB	P00752
[6]
Resource
Comments
Medline ID
PubMed ID	2012805
Journal	Biochemistry
Year	1991
Volume	30
Pages	3443-50
Authors	Fahnestock M, Woo JE, Lopez GA, Snow J, Walz DA, Arici MJ, Mobley WC
Title	beta-NGF-endopeptidase: structure and activity of a kallikrein encoded by the gene mGK-22.
Related PDB
Related UniProtKB
[7]
Resource
Comments
Medline ID
PubMed ID	1868047
Journal	Biochemistry
Year	1991
Volume	30
Pages	7666-72
Authors	Hatala MA, DiPippo VA, Powers CA
Title	Biological thiols elicit prolactin proteolysis by glandular kallikrein and permit regulation by biochemical pathways linked to redox control.
Related PDB
Related UniProtKB
[8]
Resource
Comments
Medline ID
PubMed ID	1507198
Journal	J Med Chem
Year	1992
Volume	35
Pages	3094-102
Authors	Deshpande MS, Burton J
Title	Mapping the binding site of tissue kallikrein: preparation and testing of all possible substrate analog inhibitors homologous with the sequence of kininogen between Ser386 and Gln392.
Related PDB
Related UniProtKB
[9]
Resource
Comments
Medline ID
PubMed ID	8148470
Journal	Drug Des Discov
Year	1993
Volume	10
Pages	297-317
Authors	Barth A, Wahab M, Brandt W, Frost K
Title	Classification of serine proteases derived from steric comparisons of their active sites.
Related PDB
Related UniProtKB
[10]
Resource
Comments
Medline ID
PubMed ID	8254673
Journal	J Mol Biol
Year	1993
Volume	234
Pages	779-815
Authors	Sali A, Blundell TL
Title	Comparative protein modelling by satisfaction of spatial restraints.
Related PDB
Related UniProtKB
[11]
Resource
Comments
Medline ID
PubMed ID	7526861
Journal	Biochem Biophys Res Commun
Year	1994
Volume	204
Pages	1251-6
Authors	Vihinen M
Title	Modeling of prostate specific antigen and human glandular kallikrein structures.
Related PDB
Related UniProtKB
[12]
Resource
Comments
Medline ID
PubMed ID	7749384
Journal	Braz J Med Biol Res
Year	1994
Volume	27
Pages	1935-42
Authors	Sueiras-Diaz J, Jones DM, Ashworth D, Horton J, Evans DM, Szelke M
Title	Cleavage of human kininogen fragments at Met-Lys by human tissue kallikrein.
Related PDB
Related UniProtKB
[13]
Resource
Comments
Medline ID
PubMed ID	9116171
Journal	Drug Des Discov
Year	1994
Volume	12
Pages	89-111
Authors	Barth A, Frost K, Wahab M, Brandt W, Schadler HD, Franke R
Title	Classification of serine proteases derived from steric comparisons of their active sites, part II: "Ser, His, Asp arrangements in proteolytic and nonproteolytic proteins".
Related PDB
Related UniProtKB
[14]
Resource
Comments
Medline ID
PubMed ID	7535613
Journal	Protein Sci
Year	1994
Volume	3
Pages	2033-44
Authors	Villoutreix BO, Getzoff ED, Griffin JH
Title	A structural model for the prostate disease marker, human prostate-specific antigen.
Related PDB
Related UniProtKB
[15]
Resource
Comments
Medline ID
PubMed ID	7864830
Journal	Biochem J
Year	1995
Volume	306
Pages	63-9
Authors	Chagas JR, Portaro FC, Hirata IY, Almeida PC, Juliano MA, Juliano L, Prado ES
Title	Determinants of the unusual cleavage specificity of lysyl-bradykinin-releasing kallikreins.
Related PDB
Related UniProtKB
[16]
Resource
Comments
Medline ID
PubMed ID	7538242
Journal	Urology
Year	1995
Volume	45
Pages	801-6
Authors	Bridon DP, Dowell BL
Title	Structural comparison of prostate-specific antigen and human glandular kallikrein using molecular modeling.
Related PDB
Related UniProtKB
[17]
Resource
Comments
Medline ID
PubMed ID	8961351
Journal	Protein Eng
Year	1996
Volume	9
Pages	987-95
Authors	Moreau T, Gauthier F
Title	Homology modelling of rat kallikrein rK9, a member of the tissue kallikrein family: implications for substrate specificity and inhibitor binding.
Related PDB
Related UniProtKB
[18]
Resource
Comments
Medline ID
PubMed ID	9208936
Journal	Eur J Biochem
Year	1997
Volume	246
Pages	440-6
Authors	Mikolajczyk SD, Millar LS, Marker KM, Grauer LS, Goel A, Cass MM, Kumar A, Saedi MS
Title	Ala217 is important for the catalytic function and autoactivation of prostate-specific human kallikrein 2.
Related PDB
Related UniProtKB
[19]
Resource
Comments
Medline ID
PubMed ID	9491347
Journal	J Comput Aided Mol Des
Year	1997
Volume	11
Pages	547-56
Authors	Henriques EF, Ramos MJ, Reynolds CA
Title	Inclusion of conserved buried water molecules in the model structure of rat submaxillary kallikrein.
Related PDB
Related UniProtKB
[20]
Resource
Comments	X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS).
Medline ID	97376615
PubMed ID	9232643
Journal	Protein Sci
Year	1997
Volume	6
Pages	1418-25
Authors	Timm DE
Title	The crystal structure of the mouse glandular kallikrein-13 (prorenin converting enzyme).
Related PDB	1ao5
Related UniProtKB	P36368
[21]
Resource
Comments	X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF COMPLEX WITH HIRUSTASIN.
Medline ID	97184690
PubMed ID	9032072
Journal	Structure
Year	1997
Volume	5
Pages	253-64
Authors	Mittl PR, Di Marco S, Fendrich G, Pohlig G, Heim J, Sommerhoff C, Fritz H, Priestle JP, Grutter MG
Title	A new structural class of serine protease inhibitors revealed by the structure of the hirustasin-kallikrein complex.
Related PDB	1hia
Related UniProtKB	P00752
[22]
Resource
Comments
Medline ID
PubMed ID	9568894
Journal	Protein Sci
Year	1998
Volume	7
Pages	875-85
Authors	Katz BA, Liu B, Barnes M, Springman EB
Title	Crystal structure of recombinant human tissue kallikrein at 2.0 A resolution.
Related PDB
Related UniProtKB
[23]
Resource
Comments
Medline ID
PubMed ID	9521101
Journal	Protein Sci
Year	1998
Volume	7
Pages	259-69
Authors	Piironen T, Villoutreix BO, Becker C, Hollingsworth K, Vihinen M, Bridon D, Qiu X, Rapp J, Dowell B, Lovgren T, Pettersson K, Lilja H
Title	Determination and analysis of antigenic epitopes of prostate specific antigen (PSA) and human glandular kallikrein 2 (hK2) using synthetic peptides and computer modeling.
Related PDB
Related UniProtKB
[24]
Resource
Comments
Medline ID
PubMed ID	9933620
Journal	J Biol Chem
Year	1999
Volume	274
Pages	4220-4
Authors	Kishi T, Kato M, Shimizu T, Kato K, Matsumoto K, Yoshida S, Shiosaka S, Hakoshima T
Title	Crystal structure of neuropsin, a hippocampal protease involved in kindling epileptogenesis.
Related PDB
Related UniProtKB
[25]
Resource
Comments
Medline ID
PubMed ID	10195448
Journal	J Pept Res
Year	1999
Volume	53
Pages	109-19
Authors	Juliano MA, Filira F, Gobbo M, Rocchi R, Del Nery E, Juliano L
Title	Chromogenic and fluorogenic glycosylated and acetylglycosylated peptides as substrates for serine, thiol and aspartyl proteases.
Related PDB
Related UniProtKB
[26]
Resource
Comments
Medline ID
PubMed ID	10993887
Journal	J Biol Chem
Year	2000
Volume	275
Pages	38457-66
Authors	Chen VC, Chao L, Chao J
Title	Roles of the P1, P2, and P3 residues in determining inhibitory specificity of kallistatin toward human tissue kallikrein.
Related PDB
Related UniProtKB
[27]
Resource
Comments
Medline ID
PubMed ID	10991942
Journal	J Biol Chem
Year	2000
Volume	275
Pages	40371-7
Authors	Chen VC, Chao L, Chao J
Title	A positively charged loop on the surface of kallistatin functions to enhance tissue kallikrein inhibition by acting as a secondary binding site for kallikrein.
Related PDB
Related UniProtKB
[28]
Resource
Comments
Medline ID
PubMed ID	11983703
Journal	J Biol Chem
Year	2002
Volume	277
Pages	24562-70
Authors	Bernett MJ, Blaber SI, Scarisbrick IA, Dhanarajan P, Thompson SM, Blaber M
Title	Crystal structure and biochemical characterization of human kallikrein 6 reveals that a trypsin-like kallikrein is expressed in the central nervous system.
Related PDB
Related UniProtKB
[29]
Resource
Comments
Medline ID
PubMed ID	12016211
Journal	J Biol Chem
Year	2002
Volume	277
Pages	27273-81
Authors	Gomis-Ruth FX, Bayes A, Sotiropoulou G, Pampalakis G, Tsetsenis T, Villegas V, Aviles FX, Coll M
Title	The structure of human prokallikrein 6 reveals a novel activation mechanism for the kallikrein family.
Related PDB
Related UniProtKB
[30]
Resource
Comments	NUCLEOTIDE SEQUENCE [MRNA] OF 62-252, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND X-RAY CRYSTALLOGRAPHY (1.42 ANGSTROMS) OF 25-261.
Medline ID
PubMed ID	12217694
Journal	J Mol Biol
Year	2002
Volume	322
Pages	325-37
Authors	Carvalho AL, Sanz L, Barettino D, Romero A, Calvete JJ, Romao MJ
Title	Crystal structure of a prostate kallikrein isolated from stallion seminal plasma: a homologue of human PSA.
Related PDB	1gvz
Related UniProtKB	Q6H321
[31]
Resource
Comments	X-ray Diffraction
Medline ID
PubMed ID	15651049
Journal	Proteins
Year	2005
Volume	58
Pages	802-14
Authors	Laxmikanthan G, Blaber SI, Bernett MJ, Scarisbrick IA, Juliano MA, Blaber M
Title	1.70 A X-ray structure of human apo kallikrein 1: structural changes upon peptide inhibitor/substrate binding.
Related PDB	1spj
Related UniProtKB

Comments
This enzyme belongs to the peptidase family-S1, and closely related to kallikrein (D00212 in EzCatDB). According to the literature, this enzyme has got a catalytic triad, composed of Ser/His/Asp and an oxyanionhole of mainchain amide groups. Thus, it must have a similar reaction mechanism to that of trypsin (D00197 in EzCatDB).

Created	Updated
2006-01-10	2011-02-21