DB code: D00497

CATH domain	Related DB codes (homologues)
2.40.10.10 : Thrombin, subunit H	M00139 D00214 M00167 D00426 M00133 D00428 D00429 D00430 D00431 D00432 D00433 D00434 D00435 M00227 M00209 D00194 D00197 D00211 D00212 D00216 M00212 D00224 M00217 M00216 D00528 D00848 D00850 D00851 D00852 D00855 M00152 M00155 M00157 M00181 M00315 M00316 M00317 M00348 M00349 T00074 T00410 T00411

Uniprot Enzyme Name
UniprotKB	Protein name	Synonyms	RefSeq	MEROPS	Pfam
P00759	Tonin	EC 3.4.21.35 Esterase 1 S2 kallikrein RGK-2 RSKG-5	NP_036809.1 (Protein) NM_012677.1 (DNA/RNA sequence)	S01.172 (Serine)	PF00089 (Trypsin) [Graphical View]

KEGG enzyme name
tissue kallikrein glandular kallikrein pancreatic kallikrein submandibular kallikrein submaxillary kallikrein kidney kallikrein urinary kallikrein kallikrein salivary kallikrein kininogenin kininogenase callicrein glumorin padreatin padutin kallidinogenase bradykininogenase depot-padutin urokallikrein dilminal D onokrein P

UniprotKB: Accession Number	Entry name	Activity	Subunit	Subcellular location	Cofactor
P00759	KLK2_RAT	Preferential cleavage of Arg-\|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-\|-Xaa or Leu-\|-Xaa.	Monomer.		Binds 1 zinc ion per subunit.

KEGG Pathways
Map code	Pathways	E.C.

Compound table
	Cofactors	Substrates				Products			Intermediates
KEGG-id	C00038	C00017	C00012	C00769	C00001	C00012	C01505	C00306	I00087	I00085	I00086
E.C.
Compound	Zinc	Protein	Peptide	Kininogen	H2O	Peptide	Kallidin	Bradykinin	Peptidyl-tetrahedral intermediate	Acyl-enzyme	Tetrahedral intermediate
Type	heavy metal	peptide/protein	peptide/protein	peptide/protein	H2O	peptide/protein	amino acids,amine group,aromatic ring (only carbon atom),carbohydrate,carboxyl group,imine group,lipid,peptide/protein	amino acids,amine group,aromatic ring (only carbon atom),carbohydrate,carboxyl group,imine group,lipid,peptide/protein
ChEBI	29105 29105				15377 15377		6102 6102	3165 3165
PubChem	32051 32051				22247451 962 22247451 962		5311111 5311111	439201 439201

1tonA01	Unbound	Unbound	Unbound	Unbound		Unbound	Unbound	Unbound
1tonA02	Bound:_ZN	Unbound	Unbound	Unbound		Unbound	Unbound	Unbound

Reference for Active-site residues
resource	references	E.C.

Active-site residues
PDB	Catalytic residues	Cofactor-binding residues	Modified residues	Main-chain involved in catalysis	Comment

1tonA01	SER 195			GLY 193;SER 195
1tonA02	HIS 57;ASP 102	HIS 57;HIS 97;HIS 99(Zinc binding)

References for Catalytic Mechanism
References	Sections	No. of steps in catalysis

References
[1]
Resource
Comments	X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
Medline ID	88011252
PubMed ID	2821276
Journal	J Mol Biol
Year	1987
Volume	195
Pages	373-96
Authors	Fujinaga M, James MN
Title	Rat submaxillary gland serine protease, tonin. Structure solution and refinement at 1.8 A resolution.
Related PDB	1ton
Related UniProtKB	P00759
[2]
Resource
Comments
Medline ID
PubMed ID	8148470
Journal	Drug Des Discov
Year	1993
Volume	10
Pages	297-317
Authors	Barth A, Wahab M, Brandt W, Frost K
Title	Classification of serine proteases derived from steric comparisons of their active sites.
Related PDB
Related UniProtKB
[3]
Resource
Comments
Medline ID
PubMed ID	9116171
Journal	Drug Des Discov
Year	1994
Volume	12
Pages	89-111
Authors	Barth A, Frost K, Wahab M, Brandt W, Schadler HD, Franke R
Title	Classification of serine proteases derived from steric comparisons of their active sites, part II: "Ser, His, Asp arrangements in proteolytic and nonproteolytic proteins".
Related PDB
Related UniProtKB

Comments
This enzyme belongs to the peptidase family-S1. This enzyme has got a catalytic triad composed of Ser195/His57/Asp102, similar to that of trypsin (D00197 in EzCatDB). However, His57 orients differently from other homologous peptidases, forming a binding site for zinc ion, along with His97 and His99. Thus, the zinc ion seems to play an inhibitory role, instead of a cofactor.

Created	Updated
2006-01-10	2011-02-18