DB code: D00432

CATH domain	Related DB codes (homologues)
2.40.10.10 : Thrombin, subunit H	M00139 D00214 M00167 D00426 M00133 D00428 D00429 D00430 D00431 D00433 D00434 D00435 M00227 M00209 D00194 D00197 D00211 D00212 D00216 M00212 D00224 D00497 M00217 M00216 D00528 D00848 D00850 D00851 D00852 D00855 M00152 M00155 M00157 M00181 M00315 M00316 M00317 M00348 M00349 T00074 T00410 T00411

Uniprot Enzyme Name
UniprotKB	Protein name	Synonyms	RefSeq	MEROPS	Pfam
P08419	Elastase-2A	EC 3.4.21.71 Elastase-2	NP_999274.1 (Protein) NM_214109.1 (DNA/RNA sequence)	S01.155 (Serine)	PF00089 (Trypsin) [Graphical View]
Q29461	Elastase-2A	EC 3.4.21.71 Elastase-2 Elastase II	NP_777139.1 (Protein) NM_174714.2 (DNA/RNA sequence)	S01.155 (Serine)	PF00089 (Trypsin) [Graphical View]

KEGG enzyme name
pancreatic elastase II pancreatic elastase 2

UniprotKB: Accession Number	Entry name	Activity	Subunit	Subcellular location	Cofactor
P08419	ELA2A_PIG	Preferential cleavage: Leu-\|-Xaa, Met-\|-Xaa and Phe-\|-Xaa. Hydrolyzes elastin.		Secreted.
Q29461	ELA2A_BOVIN	Preferential cleavage: Leu-\|-Xaa, Met-\|-Xaa and Phe-\|-Xaa. Hydrolyzes elastin.		Secreted.

KEGG Pathways
Map code	Pathways	E.C.

Compound table
	Substrates		Products	Intermediates
KEGG-id	C00373	C00001	C00012	I00087	I00085	I00086
E.C.
Compound	Elastin	H2O	Peptide	Peptidyl-tetrahedral intermediate	Acyl-enzyme	Tetrahedral intermediate
Type	peptide/protein	H2O	peptide/protein
ChEBI		15377 15377
PubChem	439221 439221	22247451 962 22247451 962

1bruP01	Unbound		Unbound	Unbound	Intermediate-analogue:1NB	Unbound
1bruP02	Unbound		Unbound	Unbound	Unbound	Unbound

Reference for Active-site residues
resource	references	E.C.
Swiss-prot;P08419

Active-site residues
PDB	Catalytic residues	Cofactor-binding residues	Modified residues	Main-chain involved in catalysis	Comment

1bruP01	SER 195			GLY 193;SER 195
1bruP02	HIS 57;ASP 102

References for Catalytic Mechanism
References	Sections	No. of steps in catalysis

References
[1]
Resource
Comments
Medline ID
PubMed ID	6094548
Journal	J Biol Chem
Year	1984
Volume	259
Pages	14271-8
Authors	Swift GH, Craik CS, Stary SJ, Quinto C, Lahaie RG, Rutter WJ, MacDonald RJ
Title	Structure of the two related elastase genes expressed in the rat pancreas.
Related PDB
Related UniProtKB
[2]
Resource
Comments
Medline ID
PubMed ID	3635654
Journal	J Theor Biol
Year	1986
Volume	119
Pages	107-24
Authors	Carlson GM, MacDonald RJ, Meyer EF Jr
Title	Computer aided prediction and evaluation of the tertiary structure for rat elastase II.
Related PDB
Related UniProtKB
[3]
Resource
Comments
Medline ID
PubMed ID	3634756
Journal	Int J Pept Protein Res
Year	1986
Volume	27
Pages	183-90
Authors	Vered M, Gertler A, Burstein Y
Title	Partial amino acid sequence of porcine elastase II. Active site and the activation peptide regions.
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID	11838546
Journal	J Protein Chem
Year	2001
Volume	20
Pages	577-84
Authors	Azuma K, Banshou Y, Suzuki H
Title	Bovine pancreatic elastase II cleaves Gln-Ile bond.
Related PDB
Related UniProtKB

Comments
This enzyme belongs to the peptidase family-S1. This enzyme has got a catalytic triad, composed of Ser195/His57/Asp102, suggesting that it has a similar catalytic mechanism to that of trypsin.

Created	Updated
2004-10-26	2011-02-21