DB code: D00002
| CATH domain | 3.90.180.10 : Quinone Oxidoreductase; Chain A, domain 1 | Catalytic domain |
|---|---|---|
| 3.40.50.720 : Rossmann fold | Catalytic domain | |
| E.C. | 1.1.1.2 | |
| CSA | ||
| M-CSA | ||
| MACiE | ||
| CATH domain | Related DB codes (homologues) |
|---|---|
| 3.40.50.720 : Rossmann fold | S00543 S00551 S00552 S00553 S00602 S00604 S00605 S00608 S00610 S00625 S00319 S00328 S00329 S00330 S00331 S00332 D00456 D00457 D00458 S00324 S00320 S00325 S00326 S00327 D00459 S00335 S00336 S00334 T00219 S00339 D00513 D00001 D00003 D00005 D00007 D00008 D00010 D00012 D00017 D00018 D00023 D00027 D00028 D00031 D00032 D00033 D00034 D00035 D00037 D00048 D00071 D00476 D00481 D00482 D00490 D00492 D00494 D00545 D00601 D00603 D00604 D00605 D00615 D00845 D00857 D00858 M00161 M00171 M00210 T00002 T00010 T00011 T00015 T00227 T00247 T00408 T00414 D00827 D00262 D00274 D00275 M00035 T00109 |
| 3.90.180.10 : Quinone Oxidoreductase; Chain A, domain 1 | D00001 D00018 D00048 D00481 D00482 D00490 D00492 D00615 |
| Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | Pfam |
|---|---|---|---|
| P14941 |
NADP-dependent alcohol dehydrogenase
|
EC
1.1.1.2
|
PF08240
(ADH_N)
PF00107 (ADH_zinc_N) [Graphical View] |
| P25984 |
NADP-dependent alcohol dehydrogenase
|
EC
1.1.1.2
CbADH |
PF08240
(ADH_N)
PF00107 (ADH_zinc_N) [Graphical View] |
| KEGG enzyme name |
|---|
|
alcohol dehydrogenase (NADP+)
aldehyde reductase (NADPH2) NADP-alcohol dehydrogenase NADP+-aldehyde reductase NADP+-dependent aldehyde reductase NADPH-aldehyde reductase NADPH-dependent aldehyde reductase nonspecific succinic semialdehyde reductase ALR 1 low-Km aldehyde reductase high-Km aldehyde reductase alcohol dehydrogenase (NADP+) |
| UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
|---|---|---|---|---|---|
| P14941 | ADH_THEBR | An alcohol + NADP(+) = an aldehyde + NADPH. | Homotetramer. | Binds 1 zinc ion per subunit. | |
| P25984 | ADH_CLOBE | An alcohol + NADP(+) = an aldehyde + NADPH. | Homotetramer. | Binds 1 zinc ion per subunit. |
| KEGG Pathways | Map code | Pathways | E.C. |
|---|---|---|
| MAP00010 | Glycolysis / Gluconeogenesis | |
| MAP00561 | Glycerolipid metabolism | |
| MAP00930 | Caprolactam degradation |
| Compound table | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cofactors | Substrates | Products | Intermediates | |||||||||
| KEGG-id | C00038 | C00006 | C00069 | C00005 | C00071 | C00080 | ||||||
| E.C. | ||||||||||||
| Compound | Zinc | NADP+ | Alcohol | NADPH | Aldehyde | H+ | ||||||
| Type | heavy metal | amide group,amine group,nucleotide | carbohydrate | amide group,amine group,nucleotide | carbohydrate | others | ||||||
| ChEBI |
29105 29105 |
18009 18009 |
16474 16474 |
15378 15378 |
||||||||
| PubChem |
32051 32051 |
5886 5886 |
5884 5884 |
1038 1038 |
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| 1bxzA01 |
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Bound:_ZN | Unbound | Bound:SBT | Unbound | Unbound | ||
| 1bxzB01 |
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Bound:_ZN | Unbound | Bound:SBT | Unbound | Unbound | ||
| 1bxzC01 |
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Bound:_ZN | Unbound | Bound:SBT | Unbound | Unbound | ||
| 1bxzD01 |
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Bound:_ZN | Unbound | Bound:SBT | Unbound | Unbound | ||
| 1ykfA01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | ||
| 1ykfB01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | ||
| 1ykfC01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | ||
| 1ykfD01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | ||
| 1jqbA01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | ||
| 1jqbB01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | ||
| 1jqbC01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | ||
| 1jqbD01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | ||
| 1kevA01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | ||
| 1kevB01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | ||
| 1kevC01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | ||
| 1kevD01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | ||
| 1pedA01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | ||
| 1pedB01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | ||
| 1pedC01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | ||
| 1pedD01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | ||
| 1bxzA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | ||
| 1bxzB02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | ||
| 1bxzC02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | ||
| 1bxzD02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | ||
| 1ykfA02 |
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Unbound | Bound:NAP | Unbound | Unbound | Unbound | ||
| 1ykfB02 |
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Unbound | Bound:NAP | Unbound | Unbound | Unbound | ||
| 1ykfC02 |
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Unbound | Bound:NAP | Unbound | Unbound | Unbound | ||
| 1ykfD02 |
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Unbound | Bound:NAP | Unbound | Unbound | Unbound | ||
| 1jqbA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | ||
| 1jqbB02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | ||
| 1jqbC02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | ||
| 1jqbD02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | ||
| 1kevA02 |
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Unbound | Unbound | Unbound | Bound:NDP | Unbound | ||
| 1kevB02 |
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Unbound | Unbound | Unbound | Bound:NDP | Unbound | ||
| 1kevC02 |
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Unbound | Unbound | Unbound | Bound:NDP | Unbound | ||
| 1kevD02 |
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Unbound | Unbound | Unbound | Bound:NDP | Unbound | ||
| 1pedA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | ||
| 1pedB02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | ||
| 1pedC02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | ||
| 1pedD02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | ||
| Reference for Active-site residues | ||
|---|---|---|
| resource | references | E.C. |
| Swiss-prot;P14941, P25984 & literature [4], [7] & [10] | ||
| Active-site residues | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| PDB | Catalytic residues | Cofactor-binding residues | Modified residues | Main-chain involved in catalysis | Comment | |||||
| 1bxzA01 |
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SER 39;HIS 42 | CYS 37;HIS 59;ASP 150(Catalytic zinc) | |||
| 1bxzB01 |
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SER 39;HIS 42 | CYS 37;HIS 59;ASP 150(Catalytic zinc) | |||
| 1bxzC01 |
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SER 39;HIS 42 | CYS 37;HIS 59;ASP 150(Catalytic zinc) | |||
| 1bxzD01 |
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SER 39;HIS 42 | CYS 37;HIS 59;ASP 150(Catalytic zinc) | |||
| 1ykfA01 |
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SER 39;HIS 42 | CYS 37;HIS 59;ASP 150(Catalytic zinc) | |||
| 1ykfB01 |
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SER 39;HIS 42 | CYS 37;HIS 59;ASP 150(Catalytic zinc) | |||
| 1ykfC01 |
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SER 39;HIS 42 | CYS 37;HIS 59;ASP 150(Catalytic zinc) | |||
| 1ykfD01 |
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SER 39;HIS 42 | CYS 37;HIS 59;ASP 150(Catalytic zinc) | |||
| 1jqbA01 |
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SER 1039;HIS 1042 | CYS 1037;HIS 1059;ASP 1150(Catalytic zinc) | |||
| 1jqbB01 |
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SER 2039;HIS 2042 | CYS 2037;HIS 2059;ASP 2150(Catalytic zinc) | |||
| 1jqbC01 |
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SER 3039;HIS 3042 | CYS 3037;HIS 3059;ASP 3150(Catalytic zinc) | |||
| 1jqbD01 |
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SER 4039;HIS 4042 | CYS 4037;HIS 4059;ASP 4150(Catalytic zinc) | |||
| 1kevA01 |
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SER 39;HIS 42 | CYS 37;HIS 59;ASP 150(Catalytic zinc) | |||
| 1kevB01 |
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SER 39;HIS 42 | CYS 37;HIS 59;ASP 150(Catalytic zinc) | |||
| 1kevC01 |
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SER 39;HIS 42 | CYS 37;HIS 59;ASP 150(Catalytic zinc) | |||
| 1kevD01 |
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SER 39;HIS 42 | CYS 37;HIS 59;ASP 150(Catalytic zinc) | |||
| 1pedA01 |
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SER 39;HIS 42 | CYS 37;HIS 59;ASP 150(Catalytic zinc) | |||
| 1pedB01 |
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SER 39;HIS 42 | CYS 37;HIS 59;ASP 150(Catalytic zinc) | |||
| 1pedC01 |
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SER 39;HIS 42 | CYS 37;HIS 59;ASP 150(Catalytic zinc) | |||
| 1pedD01 |
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SER 39;HIS 42 | CYS 37;HIS 59;ASP 150(Catalytic zinc) | |||
| 1bxzA02 |
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| 1bxzB02 |
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| 1bxzC02 |
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| 1bxzD02 |
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| 1ykfA02 |
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| 1ykfB02 |
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| 1ykfC02 |
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| 1ykfD02 |
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| 1jqbA02 |
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| 1jqbB02 |
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| 1jqbC02 |
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| 1jqbD02 |
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| 1kevA02 |
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| 1kevB02 |
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| 1kevC02 |
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| 1kevD02 |
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| 1pedA02 |
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| 1pedB02 |
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| 1pedC02 |
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| 1pedD02 |
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| References for Catalytic Mechanism | ||
|---|---|---|
| References | Sections | No. of steps in catalysis |
|
[4]
|
p.973-975 | |
|
[7]
|
p.621-623 | |
|
[10]
|
p.80-83 | |
| References | |
|---|---|
| [1] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8068002 |
| Journal | Biochem J |
| Year | 1994 |
| Volume | 302 |
| Pages | 163-70 |
| Authors | Burdette D, Zeikus JG |
| Title | Purification of acetaldehyde dehydrogenase and alcohol dehydrogenases from Thermoanaerobacter ethanolicus 39E and characterization of the secondary-alcohol dehydrogenase (2 degrees Adh) as a bifunctional alcohol dehydrogenase--acetyl-CoA reductive thioesterase. |
| Related PDB | |
| Related UniProtKB | |
| [2] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | |
| Journal | Acta Crystallogr D Biol Crystallogr |
| Year | 1996 |
| Volume | 52 |
| Pages | 882-6 |
| Authors | Korkhin Y, Frolow F, Bogin O, Peretz M, Kalb(Gilboa) AJ, Burstein Y |
| Title |
Crystalline alcohol dehydrogenases from the mesophilic bacterium Clostridium beijerinckii and the thermophilic bacterium Thermoanaerobium brockii: Preparation, |
| Related PDB | 1kev 1ped |
| Related UniProtKB | |
| [3] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8639709 |
| Journal | Biochim Biophys Acta |
| Year | 1996 |
| Volume | 1294 |
| Pages | 15-24 |
| Authors | Dallet S, Legoy MD |
| Title | Hydrostatic pressure induces conformational and catalytic changes on two alcohol dehydrogenases but no oligomeric dissociation. |
| Related PDB | |
| Related UniProtKB | |
| [4] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) |
| Medline ID | 98295421 |
| PubMed ID | 9836873 |
| Journal | J Mol Biol |
| Year | 1998 |
| Volume | 278 |
| Pages | 967-81 |
| Authors | Korkhin Y, Kalb(Gilboa) AJ, Peretz M, Bogin O, Burstein Y, Frolow F |
| Title |
NADP-dependent bacterial alcohol dehydrogenases: crystal structure, |
| Related PDB | 1ykf |
| Related UniProtKB | P14941 P25984 |
| [5] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10473548 |
| Journal | J Biol Chem |
| Year | 1999 |
| Volume | 274 |
| Pages | 26021-6 |
| Authors | Peralba JM, Cederlund E, Crosas B, Moreno A, Julia P, Martinez SE, Persson B, Farr s J, Pares X, Jornvall H |
| Title | Structural and enzymatic properties of a gastric NADP(H)- dependent and retinal-active alcohol dehydrogenase. |
| Related PDB | |
| Related UniProtKB | O57380 |
| [6] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10417229 |
| Journal | Protein Sci |
| Year | 1999 |
| Volume | 8 |
| Pages | 1241-9 |
| Authors | Korkhin Y, Kalb (Gilboa) AJ, Peretz M, Bogin O, Burstein Y, Frolow F |
| Title | Oligomeric integrity--the structural key to thermal stability in bacterial alcohol dehydrogenases. |
| Related PDB | |
| Related UniProtKB | |
| [7] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 10651277 |
| Journal | Proteins |
| Year | 1999 |
| Volume | 37 |
| Pages | 619-27 |
| Authors | Li C, Heatwole J, Soelaiman S, Shoham M |
| Title | Crystal structure of a thermophilic alcohol dehydrogenase substrate complex suggests determinants of substrate specificity and thermostability. |
| Related PDB | 1bxz |
| Related UniProtKB | |
| [8] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 12381840 |
| Journal | Protein Sci |
| Year | 2002 |
| Volume | 11 |
| Pages | 2561-74 |
| Authors | Bogin O, Levin I, Hacham Y, Tel-Or S, Peretz M, Frolow F, Burstein Y |
| Title | Structural basis for the enhanced thermal stability of alcohol dehydrogenase mutants from the mesophilic bacterium Clostridium beijerinckii: contribution of salt bridging. |
| Related PDB | 1jqb |
| Related UniProtKB | |
| [9] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12902331 |
| Journal | J Biol Chem |
| Year | 2003 |
| Volume | 278 |
| Pages | 40573-80 |
| Authors | Rosell A, Valencia E, Ochoa WF, Fita I, Pares X, Farres J |
| Title | Complete reversal of coenzyme specificity by concerted mutation of three consecutive residues in alcohol dehydrogenase. |
| Related PDB | |
| Related UniProtKB | |
| [10] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12818203 |
| Journal | J Mol Biol |
| Year | 2003 |
| Volume | 330 |
| Pages | 75-85 |
| Authors | Rosell A, Valencia E, Pares X, Fita I, Farres J, Ochoa WF |
| Title | Crystal structure of the vertebrate NADP(H)-dependent alcohol dehydrogenase (ADH8). |
| Related PDB | 1p0c 1p0f |
| Related UniProtKB | |
| Comments |
|---|
|
This enzyme belongs to zinc-containing alcohol dehydrogenase family.
|
| Created | Updated |
|---|---|
| 2004-05-24 | 2009-02-26 |