DB code: D00071

CATH domain 3.40.50.720 : Rossmann fold Catalytic domain
3.50.50.60 : FAD/NAD(P)-binding domain Catalytic domain
E.C. 1.18.1.2
CSA 1e6e
M-CSA 1e6e
MACiE M0142

CATH domain Related DB codes (homologues)
3.40.50.720 : Rossmann fold S00543 S00551 S00552 S00553 S00602 S00604 S00605 S00608 S00610 S00625 S00319 S00328 S00329 S00330 S00331 S00332 D00456 D00457 D00458 S00324 S00320 S00325 S00326 S00327 D00459 S00335 S00336 S00334 T00219 S00339 D00513 D00001 D00002 D00003 D00005 D00007 D00008 D00010 D00012 D00017 D00018 D00023 D00027 D00028 D00031 D00032 D00033 D00034 D00035 D00037 D00048 D00476 D00481 D00482 D00490 D00492 D00494 D00545 D00601 D00603 D00604 D00605 D00615 D00845 D00857 D00858 M00161 M00171 M00210 T00002 T00010 T00011 T00015 T00227 T00247 T00408 T00414 D00827 D00262 D00274 D00275 M00035 T00109
3.50.50.60 : FAD/NAD(P)-binding domain M00163 D00015 D00041 D00042 D00045 D00064 T00004 T00015 T00017 T00025 T00211 T00213 T00233 T00242

Uniprot Enzyme Name
UniprotKB Protein name Synonyms RefSeq
P08165 NADPH:adrenodoxin oxidoreductase, mitochondrial
Adrenodoxin reductase
AR
EC 1.18.1.2
Ferredoxin--NADP(+) reductase
Ferredoxin reductase
NP_777116.1 (Protein)
NM_174691.1 (DNA/RNA sequence)

KEGG enzyme name
ferredoxin---NADP+ reductase
adrenodoxin reductase
ferredoxin-nicotinamide adenine dinucleotide phosphate reductase
ferredoxin-NADP+ reductase
TPNH-ferredoxin reductase
ferredoxin-NADP+ oxidoreductase
NADP+:ferredoxin oxidoreductase
ferredoxin-TPN reductase
reduced nicotinamide adenine dinucleotide phosphate-adrenodoxinreductase
ferredoxin-NADP+-oxidoreductase
NADPH:ferredoxin oxidoreductase
ferredoxin-nicotinamide-adenine dinucleotide phosphate (oxidized)reductase
ferredoxin---NADP+ reductase

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P08165 ADRO_BOVIN 2 reduced ferredoxin + NADP(+) + H(+) = 2 oxidized ferredoxin + NADPH. Monomer. Interacts directly with FDX1. Mitochondrion matrix. FAD.

KEGG Pathways
Map code Pathways E.C.
MAP00195 Photosynthesis

Compound table
Cofactors Substrates Products Intermediates
KEGG-id C00176 C00138 C00006 C00139 C00005
E.C.
Compound Flavin Reduced ferredoxin NADP+ Oxidized ferredoxin NADPH
Type amide group,amine group,aromatic ring (only carbon atom),aromatic ring (with nitrogen atoms) heavy metal,peptide/protein,sulfide group amide group,amine group,nucleotide heavy metal,peptide/protein,sulfide group amide group,amine group,nucleotide
ChEBI 18009
 18009
 		16474
 16474
PubChem 5886
 5886
 		5884
 5884
1cjcA01 Bound:FAD Unbound Unbound Unbound Unbound
1e1lA01 Bound:FAD Unbound Unbound Unbound Unbound
1e1kA01 Bound:FAD Unbound Unbound Unbound Unbound
1e1mA01 Bound:FAD Unbound Unbound Unbound Unbound
1e1nA01 Bound:FAD Unbound Unbound Unbound Unbound
1e6eA01 Bound:FAD Bound:FES (chain B) Unbound Unbound Unbound
1e6eC01 Bound:FAD Bound:FES (chain D) Unbound Unbound Unbound
1cjcA02 Unbound Unbound Unbound Unbound Unbound
1e1lA02 Unbound Unbound Bound:NAP Unbound Unbound
1e1kA02 Unbound Unbound Bound:NAP Unbound Unbound
1e1mA02 Unbound Unbound Bound:NAP Unbound Unbound
1e1nA02 Unbound Unbound Unbound Unbound Unbound
1e6eA02 Unbound Unbound Unbound Unbound Unbound
1e6eC02 Unbound Unbound Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.
literature [6]

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1cjcA01
1e1lA01
1e1kA01
1e1mA01
1e1nA01
1e6eA01
1e6eC01
1cjcA02 ARG 124;TYR 331
1e1lA02 ARG 124;TYR 331
1e1kA02 ARG 124;TYR 331
1e1mA02 ARG 124;TYR 331
1e1nA02 ARG 124;TYR 331
1e6eA02 ARG 124;TYR 331
1e6eC02 ARG 124;TYR 331

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[6]
Fig.7, p.10991-10992 3
[7]
p.598-599
[8]
p.29-31
[10]
p.2789

References
[1]
Resource
Comments
Medline ID
PubMed ID 8230204
Journal J Mol Biol
Year 1993
Volume 234
Pages 245-8
Authors Kuban RJ, Marg A, Resch M, Ruckpaul K
Title Crystallization of bovine adrenodoxin reductase in a new unit cell and its crystallographic characterization.
Related PDB
Related UniProtKB
[2]
Resource
Comments
Medline ID
PubMed ID 9188745
Journal Proteins
Year 1997
Volume 28
Pages 289-92
Authors Lapko A, Muller A, Heese O, Ruckpaul K, Heinemann U
Title Preparation and crystallization of a cross-linked complex of bovine adrenodoxin and adrenodoxin reductase.
Related PDB
Related UniProtKB
[3]
Resource
Comments
Medline ID
PubMed ID 9551550
Journal Structure
Year 1998
Volume 6
Pages 269-80
Authors Muller A, Muller JJ, Muller YA, Uhlmann H, Bernhardt R, Heinemann U
Title New aspects of electron transfer revealed by the crystal structure of a truncated bovine adrenodoxin, Adx(4-108).
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID 9989598
Journal FEBS Lett
Year 1999
Volume 443
Pages 167-9
Authors Vonrhein C, Schmidt U, Ziegler GA, Schweiger S, Hanukoglu I, Schulz GE
Title Chaperone-assisted expression of authentic bovine adrenodoxin reductase in Escherichia coli.
Related PDB
Related UniProtKB
[5]
Resource
Comments X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 33-492
Medline ID 99299392
PubMed ID 10369776
Journal J Mol Biol
Year 1999
Volume 289
Pages 981-90
Authors Ziegler GA, Vonrhein C, Hanukoglu I, Schulz GE
Title The structure of adrenodoxin reductase of mitochondrial P450 systems: electron transfer for steroid biosynthesis.
Related PDB
Related UniProtKB P08165
[6]
Resource
Comments X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS)
Medline ID 20455764
PubMed ID 10998235
Journal Biochemistry
Year 2000
Volume 39
Pages 10986-95
Authors Ziegler GA, Schulz GE
Title Crystal structures of adrenodoxin reductase in complex with NADP+ and NADPH suggesting a mechanism for the electron transfer of an enzyme family.
Related PDB 1e1k 1e1m 1e1n
Related UniProtKB P08165
[7]
Resource
Comments
Medline ID
PubMed ID 10899784
Journal Proteins
Year 2000
Volume 40
Pages 590-612
Authors Grinberg AV, Hannemann F, Schiffler B, Muller J, Heinemann U, Bernhardt R
Title Adrenodoxin: structure, stability, and electron transfer properties.
Related PDB
Related UniProtKB
[8]
Resource
Comments
Medline ID
PubMed ID 11716458
Journal Arch Biochem Biophys
Year 2001
Volume 396
Pages 25-34
Authors Grinberg AV, Bernhardt R
Title Contribution of a salt bridge to the thermostability of adrenodoxin determined by site-directed mutagenesis.
Related PDB
Related UniProtKB
[9]
Resource
Comments
Medline ID
PubMed ID 11298752
Journal Eur J Biochem
Year 2001
Volume 268
Pages 2338-43
Authors Tuckey RC, McKinley AJ, Headlam MJ
Title Oxidized adrenodoxin acts as a competitive inhibitor of cytochrome P450scc in mitochondria from the human placenta.
Related PDB
Related UniProtKB
[10]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF COMPLEX WITH ADRENODOXIN
Medline ID 21264735
PubMed ID 11053423
Journal J Biol Chem
Year 2001
Volume 276
Pages 2786-9
Authors Muller JJ, Lapko A, Bourenkov G, Ruckpaul K, Heinemann U
Title Adrenodoxin reductase-adrenodoxin complex structure suggests electron transfer path in steroid biosynthesis.
Related PDB 1e6e
Related UniProtKB P08165
[11]
Resource
Comments
Medline ID
PubMed ID 12137805
Journal J Steroid Biochem Mol Biol
Year 2002
Volume 81
Pages 153-8
Authors Tuckey RC, Headlam MJ
Title Placental cytochrome P450scc (CYP11A1): comparison of catalytic properties between conditions of limiting and saturating adrenodoxin reductase.
Related PDB
Related UniProtKB
[12]
Resource
Comments
Medline ID
PubMed ID 12071965
Journal Eur J Biochem
Year 2002
Volume 269
Pages 3005-13
Authors Fischer F, Raimondi D, Aliverti A, Zanetti G
Title Mycobacterium tuberculosis FprA, a novel bacterial NADPH-ferredoxin reductase.
Related PDB
Related UniProtKB

Comments

Created Updated
2004-03-24 2009-02-26