D00002
type: Enzyme
cath: Quinone Oxidoreductase; Chain A, domain 1
cath: Rossmann fold
ec: https://purl.uniprot.org/enzyme/1.1.1.2
uniprot: P14941
uniprot: P25984
kegg enzyme: alcohol dehydrogenase (NADP+)
kegg enzyme: aldehyde reductase (NADPH2)
kegg enzyme: NADP-alcohol dehydrogenase
kegg enzyme: NADP+-aldehyde reductase
kegg enzyme: NADP+-dependent aldehyde reductase
kegg enzyme: NADPH-aldehyde reductase
kegg enzyme: NADPH-dependent aldehyde reductase
kegg enzyme: nonspecific succinic semialdehyde reductase
kegg enzyme: ALR 1
kegg pathway: Glycolysis / Gluconeogenesis
kegg pathway: Glycerolipid metabolism
kegg pathway: Caprolactam degradation
kegg cofactor: Zinc
kegg substrate: NADP+
kegg substrate: Alcohol
kegg product: NADPH
kegg product: Aldehyde
kegg product: H+
pdb: 1bxzA01
pdb: 1bxzB01
pdb: 1bxzC01
pdb: 1bxzD01
pdb: 1ykfA01
pdb: 1ykfB01
pdb: 1ykfC01
pdb: 1ykfD01
pdb: 1jqbA01
pdb: 1jqbB01
pdb: 1jqbC01
pdb: 1jqbD01
pdb: 1kevA01
pdb: 1kevB01
pdb: 1kevC01
pdb: 1kevD01
pdb: 1pedA01
pdb: 1pedB01
pdb: 1pedC01
pdb: 1pedD01
pdb: 1bxzA02
pdb: 1bxzB02
pdb: 1bxzC02
pdb: 1bxzD02
pdb: 1ykfA02
pdb: 1ykfB02
pdb: 1ykfC02
pdb: 1ykfD02
pdb: 1jqbA02
pdb: 1jqbB02
pdb: 1jqbC02
pdb: 1jqbD02
pdb: 1kevA02
pdb: 1kevB02
pdb: 1kevC02
pdb: 1kevD02
pdb: 1pedA02
pdb: 1pedB02
pdb: 1pedC02
pdb: 1pedD02
active site residues resources: Structural and enzymatic properties of a gastric NADP(H)- dependent and retinal-active alcohol dehydrogenase.
active site residues resources: Structural basis for the enhanced thermal stability of alcohol dehydrogenase mutants from the mesophilic bacterium Clostridium beijerinckii: contribution of salt bridging.
active site residues resources: http://ezcatdb.cbrc.jp/EzCatDB/reference/D00002#11
active site residues resources: P14941
active site residues resources: P25984
references: http://ezcatdb.cbrc.jp/EzCatDB/reference/D00002#1
references: Crystalline alcohol dehydrogenases from the mesophilic bacterium Clostridium beijerinckii and the thermophilic bacterium Thermoanaerobium brockii: Preparation, characterization and molecular symmetry.
references: Hydrostatic pressure induces conformational and catalytic changes on two alcohol dehydrogenases but no oligomeric dissociation.
references: NADP-dependent bacterial alcohol dehydrogenases: crystal structure, cofactor-binding and cofactor specificity of the ADHs of Clostridium beijerinckii and Thermoanaerobacter brockii.
references: Structural and enzymatic properties of a gastric NADP(H)- dependent and retinal-active alcohol dehydrogenase.
references: Oligomeric integrity--the structural key to thermal stability in bacterial alcohol dehydrogenases.
references: Crystal structure of a thermophilic alcohol dehydrogenase substrate complex suggests determinants of substrate specificity and thermostability.
references: Structural basis for the enhanced thermal stability of alcohol dehydrogenase mutants from the mesophilic bacterium Clostridium beijerinckii: contribution of salt bridging.
references: Complete reversal of coenzyme specificity by concerted mutation of three consecutive residues in alcohol dehydrogenase.
references: Crystal structure of the vertebrate NADP(H)-dependent alcohol dehydrogenase (ADH8).
comment: This enzyme belongs to zinc-containing alcohol dehydrogenase family.

Quinone Oxidoreductase; Chain A, domain 1
type: Cath
references: http://www.cathdb.info/version/latest/superfamily/3.90.180.10
catalytic domain: index.html

Rossmann fold
type: Cath
references: http://www.cathdb.info/version/latest/superfamily/3.40.50.720
catalytic domain: index.html

Glycolysis / Gluconeogenesis
type: KeggPathway
references: KEGG:MAP00010

Glycerolipid metabolism
type: KeggPathway
references: KEGG:MAP00561

Caprolactam degradation
type: KeggPathway
references: KEGG:MAP00930

Zinc
type: KeggCofactor
references: KEGG:C00038
compound type: heavy metal
references: CHEBI:29105
references: http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi

NADP+
type: KeggSubstrate
references: KEGG:C00006
compound type: amide group
compound type: amine group
compound type: nucleotide
references: CHEBI:18009
references: http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi

Alcohol
type: KeggSubstrate
references: KEGG:C00069
compound type: carbohydrate

NADPH
type: KeggProduct
references: KEGG:C00005
compound type: amide group
compound type: amine group
compound type: nucleotide
references: CHEBI:16474
references: http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi

Aldehyde
type: KeggProduct
references: KEGG:C00071
compound type: carbohydrate

H+
type: KeggProduct
references: KEGG:C00080
compound type: others
references: CHEBI:15378
references: http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi

1bxzA01
type: Pdb
references: 1bxz
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1bxz
catalytic residue: SER 39;HIS 42
cofactor binding residue: CYS 37;HIS 59;ASP 150(Catalytic zinc)

1bxzB01
type: Pdb
references: 1bxz
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1bxz
catalytic residue: SER 39;HIS 42
cofactor binding residue: CYS 37;HIS 59;ASP 150(Catalytic zinc)

1bxzC01
type: Pdb
references: 1bxz
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1bxz
catalytic residue: SER 39;HIS 42
cofactor binding residue: CYS 37;HIS 59;ASP 150(Catalytic zinc)

1bxzD01
type: Pdb
references: 1bxz
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1bxz
catalytic residue: SER 39;HIS 42
cofactor binding residue: CYS 37;HIS 59;ASP 150(Catalytic zinc)

1ykfA01
type: Pdb
references: 1ykf
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1ykf
catalytic residue: SER 39;HIS 42
cofactor binding residue: CYS 37;HIS 59;ASP 150(Catalytic zinc)

1ykfB01
type: Pdb
references: 1ykf
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1ykf
catalytic residue: SER 39;HIS 42
cofactor binding residue: CYS 37;HIS 59;ASP 150(Catalytic zinc)

1ykfC01
type: Pdb
references: 1ykf
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1ykf
catalytic residue: SER 39;HIS 42
cofactor binding residue: CYS 37;HIS 59;ASP 150(Catalytic zinc)

1ykfD01
type: Pdb
references: 1ykf
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1ykf
catalytic residue: SER 39;HIS 42
cofactor binding residue: CYS 37;HIS 59;ASP 150(Catalytic zinc)

1jqbA01
type: Pdb
references: 1jqb
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1jqb
catalytic residue: SER 1039;HIS 1042
cofactor binding residue: CYS 1037;HIS 1059;ASP 1150(Catalytic zinc)

1jqbB01
type: Pdb
references: 1jqb
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1jqb
catalytic residue: SER 2039;HIS 2042
cofactor binding residue: CYS 2037;HIS 2059;ASP 2150(Catalytic zinc)

1jqbC01
type: Pdb
references: 1jqb
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1jqb
catalytic residue: SER 3039;HIS 3042
cofactor binding residue: CYS 3037;HIS 3059;ASP 3150(Catalytic zinc)

1jqbD01
type: Pdb
references: 1jqb
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1jqb
catalytic residue: SER 4039;HIS 4042
cofactor binding residue: CYS 4037;HIS 4059;ASP 4150(Catalytic zinc)

1kevA01
type: Pdb
references: 1kev
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1kev
catalytic residue: SER 39;HIS 42
cofactor binding residue: CYS 37;HIS 59;ASP 150(Catalytic zinc)

1kevB01
type: Pdb
references: 1kev
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1kev
catalytic residue: SER 39;HIS 42
cofactor binding residue: CYS 37;HIS 59;ASP 150(Catalytic zinc)

1kevC01
type: Pdb
references: 1kev
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1kev
catalytic residue: SER 39;HIS 42
cofactor binding residue: CYS 37;HIS 59;ASP 150(Catalytic zinc)

1kevD01
type: Pdb
references: 1kev
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1kev
catalytic residue: SER 39;HIS 42
cofactor binding residue: CYS 37;HIS 59;ASP 150(Catalytic zinc)

1pedA01
type: Pdb
references: 1ped
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1ped
catalytic residue: SER 39;HIS 42
cofactor binding residue: CYS 37;HIS 59;ASP 150(Catalytic zinc)

1pedB01
type: Pdb
references: 1ped
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1ped
catalytic residue: SER 39;HIS 42
cofactor binding residue: CYS 37;HIS 59;ASP 150(Catalytic zinc)

1pedC01
type: Pdb
references: 1ped
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1ped
catalytic residue: SER 39;HIS 42
cofactor binding residue: CYS 37;HIS 59;ASP 150(Catalytic zinc)

1pedD01
type: Pdb
references: 1ped
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1ped
catalytic residue: SER 39;HIS 42
cofactor binding residue: CYS 37;HIS 59;ASP 150(Catalytic zinc)

http://ezcatdb.cbrc.jp/EzCatDB/reference/D00002#1
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/8068002
journal: Biochem J
volume: 302
pages: 163-70
published year: 1994
authors:
  item: Burdette D
  item: Zeikus JG
title: Purification of acetaldehyde dehydrogenase and alcohol dehydrogenases from Thermoanaerobacter ethanolicus 39E and characterization of the secondary-alcohol dehydrogenase (2 degrees Adh) as a bifunctional alcohol dehydrogenase--acetyl-CoA reductive thioesterase.

Crystalline alcohol dehydrogenases from the mesophilic bacterium Clostridium beijerinckii and the thermophilic bacterium Thermoanaerobium brockii: Preparation, characterization and molecular symmetry.
type: BibliographicResource
comment: X-ray crystallography
journal: Acta Crystallogr D Biol Crystallogr
volume: 52
pages: 882-6
published year: 1996
authors:
  item: Korkhin Y
  item: Frolow F
  item: Bogin O
  item: Peretz M
  item: Kalb(Gilboa) AJ
  item: Burstein Y
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl

Hydrostatic pressure induces conformational and catalytic changes on two alcohol dehydrogenases but no oligomeric dissociation.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/8639709
journal: Biochim Biophys Acta
volume: 1294
pages: 15-24
published year: 1996
authors:
  item: Dallet S
  item: Legoy MD

NADP-dependent bacterial alcohol dehydrogenases: crystal structure, cofactor-binding and cofactor specificity of the ADHs of Clostridium beijerinckii and Thermoanaerobacter brockii.
type: BibliographicResource
comment: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS)
medline id: 98295421
references: http://www.ncbi.nlm.nih.gov/pubmed/9836873
journal: J Mol Biol
volume: 278
pages: 967-81
published year: 1998
authors:
  item: Korkhin Y
  item: Kalb(Gilboa) AJ
  item: Peretz M
  item: Bogin O
  item: Burstein Y
  item: Frolow F
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: P14941
references: P25984

Structural and enzymatic properties of a gastric NADP(H)- dependent and retinal-active alcohol dehydrogenase.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/10473548
journal: J Biol Chem
volume: 274
pages: 26021-6
published year: 1999
authors:
  item: Peralba JM
  item: Cederlund E
  item: Crosas B
  item: Moreno A
  item: Julia P
  item: Martinez SE
  item: Persson B
  item: Farr s J
  item: Pares X
  item: Jornvall H
references: O57380

Oligomeric integrity--the structural key to thermal stability in bacterial alcohol dehydrogenases.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/10417229
journal: Protein Sci
volume: 8
pages: 1241-9
published year: 1999
authors:
  item: Korkhin Y
  item: Kalb (Gilboa) AJ
  item: Peretz M
  item: Bogin O
  item: Burstein Y
  item: Frolow F

Crystal structure of a thermophilic alcohol dehydrogenase substrate complex suggests determinants of substrate specificity and thermostability.
type: BibliographicResource
comment: X-ray crystallography
references: http://www.ncbi.nlm.nih.gov/pubmed/10651277
journal: Proteins
volume: 37
pages: 619-27
published year: 1999
authors:
  item: Li C
  item: Heatwole J
  item: Soelaiman S
  item: Shoham M
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl

Structural basis for the enhanced thermal stability of alcohol dehydrogenase mutants from the mesophilic bacterium Clostridium beijerinckii: contribution of salt bridging.
type: BibliographicResource
comment: X-ray crystallography
references: http://www.ncbi.nlm.nih.gov/pubmed/12381840
journal: Protein Sci
volume: 11
pages: 2561-74
published year: 2002
authors:
  item: Bogin O
  item: Levin I
  item: Hacham Y
  item: Tel-Or S
  item: Peretz M
  item: Frolow F
  item: Burstein Y
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl

Complete reversal of coenzyme specificity by concerted mutation of three consecutive residues in alcohol dehydrogenase.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/12902331
journal: J Biol Chem
volume: 278
pages: 40573-80
published year: 2003
authors:
  item: Rosell A
  item: Valencia E
  item: Ochoa WF
  item: Fita I
  item: Pares X
  item: Farres J

Crystal structure of the vertebrate NADP(H)-dependent alcohol dehydrogenase (ADH8).
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/12818203
journal: J Mol Biol
volume: 330
pages: 75-85
published year: 2003
authors:
  item: Rosell A
  item: Valencia E
  item: Pares X
  item: Fita I
  item: Farres J
  item: Ochoa WF
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl

low-Km aldehyde reductase
type: KeggEnzyme

high-Km aldehyde reductase
type: KeggEnzyme

alcohol dehydrogenase (NADP+)
type: KeggEnzyme

ReferenceForCatalyticMechanism
reference index: 4
reference section: p.973-975

ReferenceForCatalyticMechanism
reference index: 7
reference section: p.621-623

ReferenceForCatalyticMechanism
reference index: 10
reference section: p.80-83