D00071
type: Enzyme
cath: Rossmann fold
cath: FAD/NAD(P)-binding domain
ec: https://purl.uniprot.org/enzyme/1.18.1.2
csa: 1e6e
macie: M0142
uniprot: P08165
kegg enzyme: ferredoxin---NADP+ reductase
kegg enzyme: adrenodoxin reductase
kegg enzyme: ferredoxin-nicotinamide adenine dinucleotide phosphate reductase
kegg enzyme: ferredoxin-NADP+ reductase
kegg enzyme: TPNH-ferredoxin reductase
kegg enzyme: ferredoxin-NADP+ oxidoreductase
kegg enzyme: NADP+:ferredoxin oxidoreductase
kegg enzyme: ferredoxin-TPN reductase
kegg enzyme: reduced nicotinamide adenine dinucleotide phosphate-adrenodoxinreductase
kegg enzyme: ferredoxin-NADP+-oxidoreductase
kegg enzyme: NADPH:ferredoxin oxidoreductase
kegg enzyme: ferredoxin-nicotinamide-adenine dinucleotide phosphate (oxidized)reductase
kegg pathway: Photosynthesis
kegg cofactor: Flavin
kegg substrate: Reduced ferredoxin
kegg substrate: NADP+
kegg product: Oxidized ferredoxin
kegg product: NADPH
pdb: 1cjcA01
pdb: 1e1lA01
pdb: 1e1kA01
pdb: 1e1mA01
pdb: 1e1nA01
pdb: 1e6eA01
pdb: 1e6eC01
pdb: 1cjcA02
pdb: 1e1lA02
pdb: 1e1kA02
pdb: 1e1mA02
pdb: 1e1nA02
pdb: 1e6eA02
pdb: 1e6eC02
pdb bound state: Unbound
  compound: Reduced ferredoxin
  pdb: 1cjcA01
pdb bound state: Unbound
  compound: Reduced ferredoxin
  pdb: 1e1lA01
pdb bound state: Unbound
  compound: Reduced ferredoxin
  pdb: 1e1kA01
pdb bound state: Unbound
  compound: Reduced ferredoxin
  pdb: 1e1mA01
pdb bound state: Unbound
  compound: Reduced ferredoxin
  pdb: 1e1nA01
pdb bound state: Unbound
  compound: Reduced ferredoxin
  pdb: 1cjcA02
pdb bound state: Unbound
  compound: Reduced ferredoxin
  pdb: 1e1lA02
pdb bound state: Unbound
  compound: Reduced ferredoxin
  pdb: 1e1kA02
pdb bound state: Unbound
  compound: Reduced ferredoxin
  pdb: 1e1mA02
pdb bound state: Unbound
  compound: Reduced ferredoxin
  pdb: 1e1nA02
pdb bound state: Unbound
  compound: Reduced ferredoxin
  pdb: 1e6eA02
pdb bound state: Unbound
  compound: Reduced ferredoxin
  pdb: 1e6eC02
pdb bound state: Unbound
  compound: NADP+
  pdb: 1cjcA01
pdb bound state: Unbound
  compound: NADP+
  pdb: 1e1lA01
pdb bound state: Unbound
  compound: NADP+
  pdb: 1e1kA01
pdb bound state: Unbound
  compound: NADP+
  pdb: 1e1mA01
pdb bound state: Unbound
  compound: NADP+
  pdb: 1e1nA01
pdb bound state: Unbound
  compound: NADP+
  pdb: 1e6eA01
pdb bound state: Unbound
  compound: NADP+
  pdb: 1e6eC01
pdb bound state: Unbound
  compound: NADP+
  pdb: 1cjcA02
pdb bound state: Unbound
  compound: NADP+
  pdb: 1e1nA02
pdb bound state: Unbound
  compound: NADP+
  pdb: 1e6eA02
pdb bound state: Unbound
  compound: NADP+
  pdb: 1e6eC02
pdb bound state: Unbound
  compound: Flavin
  pdb: 1cjcA02
pdb bound state: Unbound
  compound: Flavin
  pdb: 1e1lA02
pdb bound state: Unbound
  compound: Flavin
  pdb: 1e1kA02
pdb bound state: Unbound
  compound: Flavin
  pdb: 1e1mA02
pdb bound state: Unbound
  compound: Flavin
  pdb: 1e1nA02
active site residues resources: Adrenodoxin: structure, stability, and electron transfer properties.
references: Crystallization of bovine adrenodoxin reductase in a new unit cell and its crystallographic characterization.
references: Preparation and crystallization of a cross-linked complex of bovine adrenodoxin and adrenodoxin reductase.
references: New aspects of electron transfer revealed by the crystal structure of a truncated bovine adrenodoxin, Adx(4-108).
references: Chaperone-assisted expression of authentic bovine adrenodoxin reductase in Escherichia coli.
references: The structure of adrenodoxin reductase of mitochondrial P450 systems: electron transfer for steroid biosynthesis.
references: Crystal structures of adrenodoxin reductase in complex with NADP+ and NADPH suggesting a mechanism for the electron transfer of an enzyme family.
references: Adrenodoxin: structure, stability, and electron transfer properties.
references: Contribution of a salt bridge to the thermostability of adrenodoxin determined by site-directed mutagenesis.
references: Oxidized adrenodoxin acts as a competitive inhibitor of cytochrome P450scc in mitochondria from the human placenta.
references: Adrenodoxin reductase-adrenodoxin complex structure suggests electron transfer path in steroid biosynthesis.
references: Placental cytochrome P450scc (CYP11A1): comparison of catalytic properties between conditions of limiting and saturating adrenodoxin reductase.
references: Mycobacterium tuberculosis FprA, a novel bacterial NADPH-ferredoxin reductase.

Rossmann fold
type: Cath
references: http://www.cathdb.info/version/latest/superfamily/3.40.50.720
catalytic domain: index.html

FAD/NAD(P)-binding domain
type: Cath
references: http://www.cathdb.info/version/latest/superfamily/3.50.50.60
catalytic domain: index.html

Photosynthesis
type: KeggPathway
references: KEGG:MAP00195

Flavin
type: KeggCofactor
references: KEGG:C00176
compound type: amide group
compound type: amine group
compound type: aromatic ring (only carbon atom)
compound type: aromatic ring (with nitrogen atoms)

Reduced ferredoxin
type: KeggSubstrate
references: KEGG:C00138
compound type: heavy metal
compound type: peptide/protein
compound type: sulfide group

NADP+
type: KeggSubstrate
references: KEGG:C00006
compound type: amide group
compound type: amine group
compound type: nucleotide
references: CHEBI:18009
references: http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi

Oxidized ferredoxin
type: KeggProduct
references: KEGG:C00139
compound type: heavy metal
compound type: peptide/protein
compound type: sulfide group

NADPH
type: KeggProduct
references: KEGG:C00005
compound type: amide group
compound type: amine group
compound type: nucleotide
references: CHEBI:16474
references: http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi

1cjcA02
type: Pdb
references: 1cjc
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1cjc
catalytic residue: ARG 124;TYR 331

1e1lA02
type: Pdb
references: 1e1l
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1e1l
catalytic residue: ARG 124;TYR 331

1e1kA02
type: Pdb
references: 1e1k
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1e1k
catalytic residue: ARG 124;TYR 331

1e1mA02
type: Pdb
references: 1e1m
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1e1m
catalytic residue: ARG 124;TYR 331

1e1nA02
type: Pdb
references: 1e1n
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1e1n
catalytic residue: ARG 124;TYR 331

1e6eA02
type: Pdb
references: 1e6e
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1e6e
catalytic residue: ARG 124;TYR 331

1e6eC02
type: Pdb
references: 1e6e
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1e6e
catalytic residue: ARG 124;TYR 331

Crystallization of bovine adrenodoxin reductase in a new unit cell and its crystallographic characterization.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/8230204
journal: J Mol Biol
volume: 234
pages: 245-8
published year: 1993
authors:
  item: Kuban RJ
  item: Marg A
  item: Resch M
  item: Ruckpaul K

Preparation and crystallization of a cross-linked complex of bovine adrenodoxin and adrenodoxin reductase.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/9188745
journal: Proteins
volume: 28
pages: 289-92
published year: 1997
authors:
  item: Lapko A
  item: Muller A
  item: Heese O
  item: Ruckpaul K
  item: Heinemann U

New aspects of electron transfer revealed by the crystal structure of a truncated bovine adrenodoxin, Adx(4-108).
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/9551550
journal: Structure
volume: 6
pages: 269-80
published year: 1998
authors:
  item: Muller A
  item: Muller JJ
  item: Muller YA
  item: Uhlmann H
  item: Bernhardt R
  item: Heinemann U

Chaperone-assisted expression of authentic bovine adrenodoxin reductase in Escherichia coli.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/9989598
journal: FEBS Lett
volume: 443
pages: 167-9
published year: 1999
authors:
  item: Vonrhein C
  item: Schmidt U
  item: Ziegler GA
  item: Schweiger S
  item: Hanukoglu I
  item: Schulz GE

The structure of adrenodoxin reductase of mitochondrial P450 systems: electron transfer for steroid biosynthesis.
type: BibliographicResource
comment: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 33-492
medline id: 99299392
references: http://www.ncbi.nlm.nih.gov/pubmed/10369776
journal: J Mol Biol
volume: 289
pages: 981-90
published year: 1999
authors:
  item: Ziegler GA
  item: Vonrhein C
  item: Hanukoglu I
  item: Schulz GE
references: P08165

Crystal structures of adrenodoxin reductase in complex with NADP+ and NADPH suggesting a mechanism for the electron transfer of an enzyme family.
type: BibliographicResource
comment: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS)
medline id: 20455764
references: http://www.ncbi.nlm.nih.gov/pubmed/10998235
journal: Biochemistry
volume: 39
pages: 10986-95
published year: 2000
authors:
  item: Ziegler GA
  item: Schulz GE
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: P08165

Adrenodoxin: structure, stability, and electron transfer properties.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/10899784
journal: Proteins
volume: 40
pages: 590-612
published year: 2000
authors:
  item: Grinberg AV
  item: Hannemann F
  item: Schiffler B
  item: Muller J
  item: Heinemann U
  item: Bernhardt R

Contribution of a salt bridge to the thermostability of adrenodoxin determined by site-directed mutagenesis.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/11716458
journal: Arch Biochem Biophys
volume: 396
pages: 25-34
published year: 2001
authors:
  item: Grinberg AV
  item: Bernhardt R

Oxidized adrenodoxin acts as a competitive inhibitor of cytochrome P450scc in mitochondria from the human placenta.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/11298752
journal: Eur J Biochem
volume: 268
pages: 2338-43
published year: 2001
authors:
  item: Tuckey RC
  item: McKinley AJ
  item: Headlam MJ

Adrenodoxin reductase-adrenodoxin complex structure suggests electron transfer path in steroid biosynthesis.
type: BibliographicResource
comment: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF COMPLEX WITH ADRENODOXIN
medline id: 21264735
references: http://www.ncbi.nlm.nih.gov/pubmed/11053423
journal: J Biol Chem
volume: 276
pages: 2786-9
published year: 2001
authors:
  item: Muller JJ
  item: Lapko A
  item: Bourenkov G
  item: Ruckpaul K
  item: Heinemann U
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: P08165

Placental cytochrome P450scc (CYP11A1): comparison of catalytic properties between conditions of limiting and saturating adrenodoxin reductase.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/12137805
journal: J Steroid Biochem Mol Biol
volume: 81
pages: 153-8
published year: 2002
authors:
  item: Tuckey RC
  item: Headlam MJ

Mycobacterium tuberculosis FprA, a novel bacterial NADPH-ferredoxin reductase.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/12071965
journal: Eur J Biochem
volume: 269
pages: 3005-13
published year: 2002
authors:
  item: Fischer F
  item: Raimondi D
  item: Aliverti A
  item: Zanetti G

ReferenceForCatalyticMechanism
reference index: 6
reference section: Fig.7, p.10991-10992
reference step: 3

ReferenceForCatalyticMechanism
reference index: 7
reference section: p.598-599

ReferenceForCatalyticMechanism
reference index: 8
reference section: p.29-31

ReferenceForCatalyticMechanism
reference index: 10
reference section: p.2789