DB code: M00161
| CATH domain | 3.40.50.720 : Rossmann fold | Catalytic domain |
|---|---|---|
| 3.40.50.1770 : Rossmann fold | Catalytic domain | |
| -.-.-.- : | ||
| 3.40.50.1220 : Rossmann fold | Catalytic domain | |
| E.C. | 1.6.1.2 | |
| CSA | ||
| M-CSA | ||
| MACiE | M0116 | |
| CATH domain | Related DB codes (homologues) |
|---|---|
| 3.40.50.1220 : Rossmann fold | M00162 T00237 T00230 |
| 3.40.50.1770 : Rossmann fold | D00031 |
| 3.40.50.720 : Rossmann fold | S00543 S00551 S00552 S00553 S00602 S00604 S00605 S00608 S00610 S00625 S00319 S00328 S00329 S00330 S00331 S00332 D00456 D00457 D00458 S00324 S00320 S00325 S00326 S00327 D00459 S00335 S00336 S00334 T00219 S00339 D00513 D00001 D00002 D00003 D00005 D00007 D00008 D00010 D00012 D00017 D00018 D00023 D00027 D00028 D00031 D00032 D00033 D00034 D00035 D00037 D00048 D00071 D00476 D00481 D00482 D00490 D00492 D00494 D00545 D00601 D00603 D00604 D00605 D00615 D00845 D00857 D00858 M00171 M00210 T00002 T00010 T00011 T00015 T00227 T00247 T00408 T00414 D00827 D00262 D00274 D00275 M00035 T00109 |
| Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | RefSeq | Pfam |
|---|---|---|---|---|
| Q2RSB2 |
NAD(P) transhydrogenase subunit alpha part 1
|
EC
1.6.1.2
Nicotinamide nucleotide transhydrogenase subunit alpha 1 Pyridine nucleotide transhydrogenase subunit alpha 1 Proton-translocating transhydrogenase component 1 dI |
YP_427270.1
(Protein)
NC_007643.1 (DNA/RNA sequence) |
PF01262
(AlaDh_PNT_C)
PF05222 (AlaDh_PNT_N) [Graphical View] |
| Q2RSB3 |
NAD(P) transhydrogenase subunit alpha part 2
|
EC
1.6.1.2
Nicotinamide nucleotide transhydrogenase subunit alpha 2 Pyridine nucleotide transhydrogenase subunit alpha 2 Proton-translocating transhydrogenase component 2 dII |
YP_427269.1
(Protein)
NC_007643.1 (DNA/RNA sequence) |
PF12769
(DUF3814)
[Graphical View] |
| Q2RSB4 |
NAD(P) transhydrogenase subunit beta
|
EC
1.6.1.2
Nicotinamide nucleotide transhydrogenase subunit beta Pyridine nucleotide transhydrogenase subunit beta Proton-translocating transhydrogenase NADP(H)-binding component dIII |
YP_427268.1
(Protein)
NC_007643.1 (DNA/RNA sequence) |
PF02233
(PNTB)
[Graphical View] |
| KEGG enzyme name |
|---|
|
NAD(P)+ transhydrogenase (AB-specific)
pyridine nucleotide transhydrogenase transhydrogenase NAD(P)+ transhydrogenase nicotinamide adenine dinucleotide (phosphate) transhydrogenase NAD+ transhydrogenase NADH transhydrogenase nicotinamide nucleotide transhydrogenase NADPH-NAD+ transhydrogenase pyridine nucleotide transferase NADPH-NAD+ oxidoreductase NADH-NADP+-transhydrogenase NADPH:NAD+ transhydrogenase H+-Thase energy-linked transhydrogenase NAD(P)+ transhydrogenase (AB-specific) |
| UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
|---|---|---|---|---|---|
| Q2RSB2 | PNTAA_RHORT | NADPH + NAD(+) = NADP(+) + NADH. | Complex of an alpha and a beta chain, in Rhodospirillum, the alpha chain seems to be made of two subunits (By similarity). | ||
| Q2RSB3 | PNTAB_RHORT | NADPH + NAD(+) = NADP(+) + NADH. | Complex of an alpha and a beta chain, in Rhodospirillum, the alpha chain seems to be made of two subunits (By similarity). | Cell inner membrane, Multi-pass membrane protein (By similarity). | |
| Q2RSB4 | PNTB_RHORT | NADPH + NAD(+) = NADP(+) + NADH. | Complex of an alpha and a beta chain, in Rhodospirillum, the alpha chain seems to be made of two subunits (By similarity). | Cell inner membrane, Multi-pass membrane protein (By similarity). |
| KEGG Pathways | Map code | Pathways | E.C. |
|---|---|---|
| MAP00760 | Nicotinate and nicotinamide metabolism |
| Compound table | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Substrates | Products | Intermediates | ||||||||
| KEGG-id | C00005 | C00003 | C00006 | C00004 | ||||||
| E.C. | ||||||||||
| Compound | NADPH | NAD+ | NADP+ | NADH | ||||||
| Type | amide group,amine group,nucleotide | amide group,amine group,nucleotide | amide group,amine group,nucleotide | amide group,amine group,nucleotide | ||||||
| ChEBI |
16474 16474 |
15846 15846 |
18009 18009 |
16908 16908 |
||||||
| PubChem |
5884 5884 |
5893 5893 |
5886 5886 |
439153 439153 |
||||||
| 1f8gA01 |
|
|
|
|
|
Unbound | Bound:NAD | Unbound | Unbound | |
| 1f8gB01 |
|
|
|
|
|
Unbound | Analogue:NAD | Unbound | Unbound | |
| 1f8gC01 |
|
|
|
|
|
Unbound | Analogue:NAD | Unbound | Unbound | |
| 1f8gD01 |
|
|
|
|
|
Unbound | Bound:NAD | Unbound | Unbound | |
| 1l7dA01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | |
| 1l7dB01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | |
| 1l7dC01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | |
| 1l7dD01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | |
| 1l7eA01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Bound:NAI | |
| 1l7eB01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | |
| 1l7eC01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | |
| 1l7eD01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Bound:NAI | |
| 1hzzA01 |
|
|
|
|
|
Unbound | Bound:NAD | Unbound | Unbound | |
| 1hzzB01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | |
| 1nm5A01 |
|
|
|
|
|
Unbound | Bound:NAD | Unbound | Unbound | |
| 1nm5B01 |
|
|
|
|
|
Unbound | Analogue:NAD | Unbound | Unbound | |
| 1ptjA01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | |
| 1ptjB01 |
|
|
|
|
|
Unbound | Analogue:SND | Unbound | Unbound | |
| 1u28A01 |
|
|
|
|
|
Unbound | Bound:NAD | Unbound | Unbound | |
| 1u28B01 |
|
|
|
|
|
Unbound | Analogue:NAD | Unbound | Unbound | |
| 1u2dA01 |
|
|
|
|
|
Unbound | Bound:NAD | Unbound | Unbound | |
| 1u2dB01 |
|
|
|
|
|
Unbound | Bound:NAD | Unbound | Unbound | |
| 1u2gA01 |
|
|
|
|
|
Unbound | Analogue:APR | Unbound | Unbound | |
| 1u2gB01 |
|
|
|
|
|
Unbound | Analogue:APR | Unbound | Unbound | |
| 1f8gA02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | |
| 1f8gB02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | |
| 1f8gC02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | |
| 1f8gD02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | |
| 1l7dA02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | |
| 1l7dB02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | |
| 1l7dC02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | |
| 1l7dD02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | |
| 1l7eA02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | |
| 1l7eB02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | |
| 1l7eC02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | |
| 1l7eD02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | |
| 1hzzA02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | |
| 1hzzB02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | |
| 1nm5A02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | |
| 1nm5B02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | |
| 1ptjA02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | |
| 1ptjB02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | |
| 1u28A02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | |
| 1u28B02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | |
| 1u2dA02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | |
| 1u2dB02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | |
| 1u2gA02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | |
| 1u2gB02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | |
| 1e3tA |
|
|
|
|
|
Unbound | Unbound | Bound:NAP | Unbound | |
| 1pnoA |
|
|
|
|
|
Unbound | Unbound | Bound:NAP | Unbound | |
| 1pnoB |
|
|
|
|
|
Unbound | Unbound | Bound:NAP | Unbound | |
| 1pnqA |
|
|
|
|
|
Bound:NDP | Unbound | Unbound | Unbound | |
| 1pnqB |
|
|
|
|
|
Bound:NDP | Unbound | Unbound | Unbound | |
| 1hzzC |
|
|
|
|
|
Unbound | Unbound | Bound:NAP | Unbound | |
| 1nm5C |
|
|
|
|
|
Unbound | Unbound | Bound:NAP | Unbound | |
| 1ptjC |
|
|
|
|
|
Unbound | Unbound | Bound:NAP | Unbound | |
| 1u28C |
|
|
|
|
|
Unbound | Unbound | Bound:NAP | Unbound | |
| 1u2dC |
|
|
|
|
|
Bound:NDP | Unbound | Unbound | Unbound | |
| 1u2gC |
|
|
|
|
|
Bound:NDP | Unbound | Unbound | Unbound | |
| Reference for Active-site residues | ||
|---|---|---|
| resource | references | E.C. |
| literature [6], [10] & [12] | ||
| Active-site residues | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| PDB | Catalytic residues | Cofactor-binding residues | Modified residues | Main-chain involved in catalysis | Comment | |||||
| 1f8gA01 |
|
|
|
|
|
TYR 235 | ||||
| 1f8gB01 |
|
|
|
|
|
TYR 235 | ||||
| 1f8gC01 |
|
|
|
|
|
TYR 235 | ||||
| 1f8gD01 |
|
|
|
|
|
TYR 235 | ||||
| 1l7dA01 |
|
|
|
|
|
invisible Y235 | ||||
| 1l7dB01 |
|
|
|
|
|
invisible Y235 | ||||
| 1l7dC01 |
|
|
|
|
|
invisible Y235 | ||||
| 1l7dD01 |
|
|
|
|
|
invisible Y235 | ||||
| 1l7eA01 |
|
|
|
|
|
TYR 235 | ||||
| 1l7eB01 |
|
|
|
|
|
invisible Y635 | ||||
| 1l7eC01 |
|
|
|
|
|
invisible Y1035 | ||||
| 1l7eD01 |
|
|
|
|
|
TYR 1435 | ||||
| 1hzzA01 |
|
|
|
|
|
TYR 235 | ||||
| 1hzzB01 |
|
|
|
|
|
invisible Y235 | ||||
| 1nm5A01 |
|
|
|
|
|
invisible Y235 | ||||
| 1nm5B01 |
|
|
|
|
|
invisible Y235 | ||||
| 1ptjA01 |
|
|
|
|
|
TYR 235 | ||||
| 1ptjB01 |
|
|
|
|
|
invisible Y235 | ||||
| 1u28A01 |
|
|
|
|
|
TYR 235 | ||||
| 1u28B01 |
|
|
|
|
|
invisible Y235 | ||||
| 1u2dA01 |
|
|
|
|
|
TYR 235 | ||||
| 1u2dB01 |
|
|
|
|
|
TYR 235 | ||||
| 1u2gA01 |
|
|
|
|
|
TYR 235 | ||||
| 1u2gB01 |
|
|
|
|
|
invisible Y235 | ||||
| 1f8gA02 |
|
|
|
|
|
ARG 127;GLN 132;ASP 135;SER 138 | ||||
| 1f8gB02 |
|
|
|
|
|
ARG 127;GLN 132;ASP 135;SER 138 | ||||
| 1f8gC02 |
|
|
|
|
|
ARG 127;GLN 132;ASP 135;SER 138 | ||||
| 1f8gD02 |
|
|
|
|
|
ARG 127;GLN 132;ASP 135;SER 138 | ||||
| 1l7dA02 |
|
|
|
|
|
ARG 127;GLN 132;ASP 135;SER 138 | ||||
| 1l7dB02 |
|
|
|
|
|
ARG 527;GLN 532;ASP 535;SER 538 | ||||
| 1l7dC02 |
|
|
|
|
|
ARG 927;GLN 932;ASP 935;SER 938 | ||||
| 1l7dD02 |
|
|
|
|
|
ARG 1327;GLN 1332;ASP 1335;SER 1338 | ||||
| 1l7eA02 |
|
|
|
|
|
ARG 127;GLN 132;ASP 135;SER 138 | ||||
| 1l7eB02 |
|
|
|
|
|
ARG 527;GLN 532;ASP 535;SER 538 | ||||
| 1l7eC02 |
|
|
|
|
|
ARG 927;GLN 932;ASP 935;SER 938 | ||||
| 1l7eD02 |
|
|
|
|
|
ARG 1327;GLN 1332;ASP 1335;SER 1338 | ||||
| 1hzzA02 |
|
|
|
|
|
ARG 127;GLN 132;ASP 135;SER 138 | ||||
| 1hzzB02 |
|
|
|
|
|
ARG 127;GLN 132;ASP 135;SER 138 | ||||
| 1nm5A02 |
|
|
|
|
|
ARG 127;;ASP 135;SER 138 | mutant Q132N | |||
| 1nm5B02 |
|
|
|
|
|
ARG 127;;ASP 135;SER 138 | mutant Q132N | |||
| 1ptjA02 |
|
|
|
|
|
ARG 127;GLN 132;ASP 135;SER 138 | ||||
| 1ptjB02 |
|
|
|
|
|
ARG 127;GLN 132;ASP 135;SER 138 | ||||
| 1u28A02 |
|
|
|
|
|
ARG 127;GLN 132;ASP 135;SER 138 | ||||
| 1u28B02 |
|
|
|
|
|
ARG 127;GLN 132;ASP 135;SER 138 | ||||
| 1u2dA02 |
|
|
|
|
|
ARG 127;GLN 132;ASP 135;SER 138 | ||||
| 1u2dB02 |
|
|
|
|
|
ARG 127;GLN 132;ASP 135;SER 138 | ||||
| 1u2gA02 |
|
|
|
|
|
ARG 127;GLN 132;ASP 135;SER 138 | ||||
| 1u2gB02 |
|
|
|
|
|
ARG 127;GLN 132;ASP 135;SER 138 | ||||
| 1e3tA |
|
|
|
|
|
TYR 55;ARG 90;TYR 171 | ||||
| 1pnoA |
|
|
|
|
|
TYR 316;ARG 351;TYR 432 | ||||
| 1pnoB |
|
|
|
|
|
TYR 316;ARG 351;TYR 432 | ||||
| 1pnqA |
|
|
|
|
|
TYR 316;ARG 351;TYR 432 | ||||
| 1pnqB |
|
|
|
|
|
TYR 316;ARG 351;TYR 432 | ||||
| 1hzzC |
|
|
|
|
|
TYR 55;ARG 90;TYR 171 | ||||
| 1nm5C |
|
|
|
|
|
TYR 55;ARG 90;TYR 171 | ||||
| 1ptjC |
|
|
|
|
|
TYR 55;ARG 90;TYR 171 | ||||
| 1u28C |
|
|
|
|
|
TYR 55;ARG 90;TYR 171 | ||||
| 1u2dC |
|
|
|
|
|
TYR 55;ARG 90;TYR 171 | ||||
| 1u2gC |
|
|
|
|
|
TYR 55;ARG 90;TYR 171 | ||||
| References for Catalytic Mechanism | ||
|---|---|---|
| References | Sections | No. of steps in catalysis |
|
[6]
|
p.5-6 | |
|
[10]
|
p.813 | |
|
[12]
|
p.44759-44760 | |
|
[13]
|
p.30684-30685 | |
|
[14]
|
p.167-173 | |
|
[16]
|
||
|
[17]
|
Fig.8, p.12152 | |
|
[18]
|
p.1223-1225 | |
|
[19]
|
||
|
[21]
|
||
|
[22]
|
||
| References | |
|---|---|
| [1] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8011636 |
| Journal | Biochemistry |
| Year | 1994 |
| Volume | 33 |
| Pages | 7691-700 |
| Authors | Holmberg E, Olausson T, Hultman T, Rydstrom J, Ahmad S, Glavas NA, Bragg PD |
| Title | Prediction and site-specific mutagenesis of residues in transmembrane alpha-helices of proton-pumping nicotinamide nucleotide transhydrogenases from Escherichia coli and bovine heart mitochondria. |
| Related PDB | |
| Related UniProtKB | |
| [2] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9804876 |
| Journal | Biochim Biophys Acta |
| Year | 1998 |
| Volume | 1409 |
| Pages | 25-38 |
| Authors | Gupta S, Quirk PG, Venning JD, Slade J, Bizouarn T, Grimley RL, Cotton NP, Jackson JB |
| Title | Mutation of amino acid residues in the mobile loop region of the NAD(H)-binding domain of proton-translocating transhydrogenase. |
| Related PDB | |
| Related UniProtKB | |
| [3] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10393090 |
| Journal | Biochem J |
| Year | 1999 |
| Volume | 341 |
| Pages | 329-37 |
| Authors | Venning JD, Jackson JB |
| Title | A shift in the equilibrium constant at the catalytic site of proton-translocating transhydrogenase: significance for a 'binding-change' mechanism. |
| Related PDB | |
| Related UniProtKB | |
| [4] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10514549 |
| Journal | Biochim Biophys Acta |
| Year | 1999 |
| Volume | 1413 |
| Pages | 81-91 |
| Authors | Peake SJ, Venning JD, Cotton NP, Jackson JB |
| Title | Evidence for the stabilization of NADPH relative to NADP(+) on the dIII components of proton-translocating transhydrogenases from Homo sapiens and from Rhodospirillum rubrum by measurement of tryptophan fluorescence. |
| Related PDB | |
| Related UniProtKB | |
| [5] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10216162 |
| Journal | Biochim Biophys Acta |
| Year | 1999 |
| Volume | 1411 |
| Pages | 159-69 |
| Authors | Peake SJ, Venning JD, Jackson JB |
| Title |
A catalytically active complex formed from the recombinant dI protein of Rhodospirillum rubrum transhydrogenase, |
| Related PDB | |
| Related UniProtKB | |
| [6] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10611473 |
| Journal | FEBS Lett |
| Year | 1999 |
| Volume | 464 |
| Pages | 1-8 |
| Authors | Jackson JB, Peake SJ, White SA |
| Title | Structure and mechanism of proton-translocating transhydrogenase. |
| Related PDB | |
| Related UniProtKB | |
| [7] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10587945 |
| Journal | Microb Comp Genomics |
| Year | 1999 |
| Volume | 4 |
| Pages | 173-86 |
| Authors | Studley WK, Yamaguchi M, Hatefi Y, Saier MH Jr |
| Title | Phylogenetic analyses of proton-translocating transhydrogenases. |
| Related PDB | |
| Related UniProtKB | |
| [8] | |
| Resource | |
| Comments | NMR Structure |
| Medline ID | |
| PubMed ID | 11004437 |
| Journal | Biochim Biophys Acta |
| Year | 2000 |
| Volume | 1459 |
| Pages | 248-57 |
| Authors | Jeeves M, Smith KJ, Quirk PG, Cotton NP, Jackson JB |
| Title | Solution structure of the NADP(H)-binding component (dIII) of proton-translocating transhydrogenase from Rhodospirillum rubrum. |
| Related PDB | 1e3t |
| Related UniProtKB | |
| [9] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10747934 |
| Journal | J Biol Chem |
| Year | 2000 |
| Volume | 275 |
| Pages | 19490-7 |
| Authors | Venning JD, Peake SJ, Quirk PG, Jackson JB |
| Title | Stopped-flow reaction kinetics of recombinant components of proton-translocating transhydrogenase with physiological nucleotides. |
| Related PDB | |
| Related UniProtKB | |
| [10] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10997900 |
| Journal | Structure Fold Des |
| Year | 2000 |
| Volume | 8 |
| Pages | 809-15 |
| Authors | Buckley PA, Baz Jackson J, Schneider T, White SA, Rice DW, Baker PJ |
| Title |
Protein-protein recognition, |
| Related PDB | 1f8g |
| Related UniProtKB | |
| [11] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11231296 |
| Journal | Eur J Biochem |
| Year | 2001 |
| Volume | 268 |
| Pages | 1430-8 |
| Authors | Rodrigues DJ, Venning JD, Quirk PG, Jackson JB |
| Title | A change in ionization of the NADP(H)-binding component (dIII) of proton-translocating transhydrogenase regulates both hydride transfer and nucleotide release. |
| Related PDB | |
| Related UniProtKB | |
| [12] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11577115 |
| Journal | J Biol Chem |
| Year | 2001 |
| Volume | 276 |
| Pages | 44757-61 |
| Authors | Pinheiro TJ, Venning JD, Jackson JB |
| Title | Fast hydride transfer in proton-translocating transhydrogenase revealed in a rapid mixing continuous flow device. |
| Related PDB | |
| Related UniProtKB | |
| [13] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11399770 |
| Journal | J Biol Chem |
| Year | 2001 |
| Volume | 276 |
| Pages | 30678-85 |
| Authors | Venning JD, Rodrigues DJ, Weston CJ, Cotton NP, Quirk PG, Errington N, Finet S, White SA, Jackson JB |
| Title | The heterotrimer of the membrane-peripheral components of transhydrogenase and the alternating-site mechanism of proton translocation. |
| Related PDB | |
| Related UniProtKB | |
| [14] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11250201 |
| Journal | Structure (Camb) |
| Year | 2001 |
| Volume | 9 |
| Pages | 165-76 |
| Authors | Cotton NP, White SA, Peake SJ, McSweeney S, Jackson JB |
| Title | The crystal structure of an asymmetric complex of the two nucleotide binding components of proton-translocating transhydrogenase. |
| Related PDB | 1hzz |
| Related UniProtKB | |
| [15] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12206884 |
| Journal | Biochim Biophys Acta |
| Year | 2002 |
| Volume | 1555 |
| Pages | 8-13 |
| Authors | Rodrigues DJ, Jackson JB |
| Title | A conformational change in the isolated NADP(H)-binding component (dIII) of transhydrogenase induced by low pH: a reflection of events during proton translocation by the complete enzyme? |
| Related PDB | |
| Related UniProtKB | |
| [16] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12379117 |
| Journal | Biochemistry |
| Year | 2002 |
| Volume | 41 |
| Pages | 12745-54 |
| Authors | Prasad GS, Wahlberg M, Sridhar V, Sundaresan V, Yamaguchi M, Hatefi Y, Stout CD |
| Title | Crystal structures of transhydrogenase domain I with and without bound NADH. |
| Related PDB | 1l7d 1l7e |
| Related UniProtKB | |
| [17] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 14567675 |
| Journal | Biochemistry |
| Year | 2003 |
| Volume | 42 |
| Pages | 12143-53 |
| Authors | Sundaresan V, Yamaguchi M, Chartron J, Stout CD |
| Title | Conformational change in the NADP(H) binding domain of transhydrogenase defines four states. |
| Related PDB | 1pno 1pnq |
| Related UniProtKB | |
| [18] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12564924 |
| Journal | Biochemistry |
| Year | 2003 |
| Volume | 42 |
| Pages | 1217-26 |
| Authors | van Boxel GI, Quirk PG, Cotton NP, White SA, Jackson JB |
| Title | Glutamine 132 in the NAD(H)-binding component of proton-translocating transhydrogenase tethers the nucleotides before hydride transfer. |
| Related PDB | 1nm5 |
| Related UniProtKB | |
| [19] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12765762 |
| Journal | Biochim Biophys Acta |
| Year | 2003 |
| Volume | 1604 |
| Pages | 55-9 |
| Authors | Pedersen A, Karlsson J, Althage M, Rydstrom J |
| Title | Properties of the apo-form of the NADP(H)-binding domain III of proton-pumping Escherichia coli transhydrogenase: implications for the reaction mechanism of the intact enzyme. |
| Related PDB | |
| Related UniProtKB | |
| [20] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12972415 |
| Journal | J Biol Chem |
| Year | 2003 |
| Volume | 278 |
| Pages | 47578-84 |
| Authors | Broos J, Gabellieri E, van Boxel GI, Jackson JB, Strambini GB |
| Title | Tryptophan phosphorescence spectroscopy reveals that a domain in the NAD(H)-binding component (dI) of transhydrogenase from Rhodospirillum rubrum has an extremely rigid and conformationally homogeneous protein core. |
| Related PDB | |
| Related UniProtKB | |
| [21] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 12791694 |
| Journal | J Biol Chem |
| Year | 2003 |
| Volume | 278 |
| Pages | 33208-16 |
| Authors | Singh A, Venning JD, Quirk PG, van Boxel GI, Rodrigues DJ, White SA, Jackson JB |
| Title | Interactions between transhydrogenase and thio-nicotinamide Analogues of NAD(H) and NADP(H) underline the importance of nucleotide conformational changes in coupling to proton translocation. |
| Related PDB | 1pt9 1ptj |
| Related UniProtKB | |
| [22] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 15323555 |
| Journal | Biochemistry |
| Year | 2004 |
| Volume | 43 |
| Pages | 10952-64 |
| Authors | Mather OC, Singh A, van Boxel GI, White SA, Jackson JB |
| Title | Active-site conformational changes associated with hydride transfer in proton-translocating transhydrogenase. |
| Related PDB | 1u28 1u2d 1u2g 1u31 |
| Related UniProtKB | |
| Comments |
|---|
|
This enzyme is composed of two alpha chains and a beta chain. |
| Created | Updated |
|---|---|
| 2004-12-17 | 2009-02-26 |