DB code: D00274
| RLCP classification | 9.1050.440000.8010 : Hydride transfer | |
|---|---|---|
| 9.5010.536200.8010 : Hydride transfer | ||
| CATH domain | 3.90.25.10 : UDP-galactose 4-epimerase; domain 1 | |
| 3.40.50.720 : Rossmann fold | Catalytic domain | |
| E.C. | 5.1.3.2 | |
| CSA | ||
| M-CSA | ||
| MACiE | M0188 | |
| CATH domain | Related DB codes (homologues) |
|---|---|
| 3.40.50.720 : Rossmann fold | S00543 S00551 S00552 S00553 S00602 S00604 S00605 S00608 S00610 S00625 S00319 S00328 S00329 S00330 S00331 S00332 D00456 D00457 D00458 S00324 S00320 S00325 S00326 S00327 D00459 S00335 S00336 S00334 T00219 S00339 D00513 D00001 D00002 D00003 D00005 D00007 D00008 D00010 D00012 D00017 D00018 D00023 D00027 D00028 D00031 D00032 D00033 D00034 D00035 D00037 D00048 D00071 D00476 D00481 D00482 D00490 D00492 D00494 D00545 D00601 D00603 D00604 D00605 D00615 D00845 D00857 D00858 M00161 M00171 M00210 T00002 T00010 T00011 T00015 T00227 T00247 T00408 T00414 D00827 D00262 D00275 M00035 T00109 |
| 3.90.25.10 : UDP-galactose 4-epimerase; domain 1 | D00513 D00601 D00604 D00262 D00275 |
| Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | RefSeq | Pfam |
|---|---|---|---|---|
| P09147 |
UDP-glucose 4-epimerase
|
EC
5.1.3.2
Galactowaldenase UDP-galactose 4-epimerase |
NP_415280.3
(Protein)
NC_000913.2 (DNA/RNA sequence) YP_489032.1 (Protein) NC_007779.1 (DNA/RNA sequence) |
PF01370
(Epimerase)
PF13950 (Epimerase_Csub) [Graphical View] |
| Q81K34 |
|
UDP-glucose 4-epimerase
EC 5.1.3.2 |
NP_847665.1
(Protein)
NC_003997.3 (DNA/RNA sequence) YP_022172.1 (Protein) NC_007530.2 (DNA/RNA sequence) YP_031353.1 (Protein) NC_005945.1 (DNA/RNA sequence) |
PF01370
(Epimerase)
PF13950 (Epimerase_Csub) [Graphical View] |
| Q3JPI3 |
|
UDP-glucose 4-epimerase
EC 5.1.3.2 |
YP_334520.1
(Protein)
NC_007434.1 (DNA/RNA sequence) |
PF01370
(Epimerase)
PF13950 (Epimerase_Csub) [Graphical View] |
| Q8T8E9 |
|
UDP-galactose 4-epimerase
EC 5.1.3.2 |
PF01370
(Epimerase)
PF13950 (Epimerase_Csub) [Graphical View] |
|
| Q14376 |
UDP-glucose 4-epimerase
|
EC
5.1.3.2
Galactowaldenase UDP-galactose 4-epimerase |
NP_000394.2
(Protein)
NM_000403.3 (DNA/RNA sequence) NP_001008217.1 (Protein) NM_001008216.1 (DNA/RNA sequence) NP_001121093.1 (Protein) NM_001127621.1 (DNA/RNA sequence) |
PF01370
(Epimerase)
PF13950 (Epimerase_Csub) [Graphical View] |
| KEGG enzyme name |
|---|
|
UDP-glucose 4-epimerase
UDP-galactose 4-epimerase uridine diphosphoglucose epimerase galactowaldenase UDPG-4-epimerase uridine diphosphate galactose 4-epimerase uridine diphospho-galactose-4-epimerase UDP-glucose epimerase UDP-galactose 4-epimerase 4-epimerase UDPG-4-epimerase uridine diphosphoglucose 4-epimerase uridine diphosphate glucose 4-epimerase UDP-D-galactose 4-epimerase |
| UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
|---|---|---|---|---|---|
| P09147 | GALE_ECOLI | UDP-glucose = UDP-galactose. | Homodimer. | NAD. | |
| Q81K34 | Q81K34_BACAN | ||||
| Q3JPI3 | Q3JPI3_BURP1 | ||||
| Q8T8E9 | Q8T8E9_9TRYP | ||||
| Q14376 | GALE_HUMAN | UDP-glucose = UDP-galactose. | Homodimer. | NAD. |
| KEGG Pathways | Map code | Pathways | E.C. |
|---|---|---|
| MAP00052 | Galactose metabolism | |
| MAP00520 | Nucleotide sugars metabolism |
| Compound table | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Cofactors | Substrates | Products | Intermediates | ||||||
| KEGG-id | C00003 | C00029 | C00052 | I00016 | |||||
| E.C. | |||||||||
| Compound | NAD+ | UDP-glucose | UDP-galactose | UDP-4-ketohexopyranose | |||||
| Type | amide group,amine group,nucleotide | amide group,carbohydrate,nucleotide | amide group,carbohydrate,nucleotide | ||||||
| ChEBI |
15846 15846 |
46229 46229 |
|||||||
| PubChem |
5893 5893 |
8629 8629 |
23724458 23724458 |
||||||
| 1a9yA01 |
|
|
|
|
|
Unbound | Bound:UPG | Unbound | |
| 1a9zA01 |
|
|
|
|
|
Unbound | Unbound | Bound:UPG | |
| 1kvqA01 |
|
|
|
|
|
Unbound | Bound:UPG | Unbound | |
| 1kvrA01 |
|
|
|
|
|
Unbound | Analogue:UDP | Unbound | |
| 1kvsA01 |
|
|
|
|
|
Unbound | Bound:UPG | Unbound | |
| 1kvtA01 |
|
|
|
|
|
Unbound | Bound:UPG | Unbound | |
| 1kvuA01 |
|
|
|
|
|
Unbound | Bound:UPG | Unbound | |
| 1lrjA01 |
|
|
|
|
|
Unbound | Analogue:UD1 | Unbound | |
| 1lrkA01 |
|
|
|
|
|
Unbound | Analogue:UD1 | Unbound | |
| 1lrlA01 |
|
|
|
|
|
Unbound | Bound:UPG | Unbound | |
| 1nahA01 |
|
|
|
|
|
Unbound | Analogue:UDP | Unbound | |
| 1naiA01 |
|
|
|
|
|
Unbound | Analogue:UDP | Unbound | |
| 1udaA01 |
|
|
|
|
|
Unbound | Unbound | Analogue:UFG | |
| 1udbA01 |
|
|
|
|
|
Unbound | Analogue:UFG | Unbound | |
| 1udcA01 |
|
|
|
|
|
Unbound | Analogue:UFM | Unbound | |
| 1xelA01 |
|
|
|
|
|
Unbound | Bound:UPG | Unbound | |
| 2udpA01 |
|
|
|
|
|
Unbound | Analogue:UPP | Unbound | |
| 2udpB01 |
|
|
|
|
|
Unbound | Analogue:UPP | Unbound | |
| 3enkA02 |
|
|
|
|
|
Unbound | Analogue:GUD | Unbound | |
| 3enkB02 |
|
|
|
|
|
Unbound | Analogue:GUD | Unbound | |
| 1gy8A01 |
|
|
|
|
|
Unbound | Analogue:UDP | Unbound | |
| 1gy8B01 |
|
|
|
|
|
Unbound | Analogue:UDP | Unbound | |
| 1gy8C01 |
|
|
|
|
|
Unbound | Analogue:UDP | Unbound | |
| 1gy8D01 |
|
|
|
|
|
Unbound | Analogue:UDP | Unbound | |
| 2cnbA01 |
|
|
|
|
|
Unbound | Unbound | Analogue:UFG | |
| 2cnbB01 |
|
|
|
|
|
Unbound | Unbound | Analogue:UFG | |
| 2cnbC01 |
|
|
|
|
|
Unbound | Unbound | Analogue:UFG | |
| 2cnbD01 |
|
|
|
|
|
Unbound | Unbound | Analogue:UFG | |
| 1ek5A01 |
|
|
|
|
|
Unbound | Unbound | Unbound | |
| 1ek6A01 |
|
|
|
|
|
Unbound | Bound:UPG | Unbound | |
| 1ek6B01 |
|
|
|
|
|
Unbound | Bound:UPG | Unbound | |
| 1hzjA01 |
|
|
|
|
|
Unbound | Analogue:UD1 | Unbound | |
| 1hzjB01 |
|
|
|
|
|
Unbound | Analogue:UD1 | Unbound | |
| 1i3kA01 |
|
|
|
|
|
Unbound | Bound:UPG | Unbound | |
| 1i3kB01 |
|
|
|
|
|
Unbound | Bound:UPG | Unbound | |
| 1i3lA01 |
|
|
|
|
|
Unbound | Unbound | Bound:GDU | |
| 1i3lB01 |
|
|
|
|
|
Unbound | Unbound | Bound:GDU | |
| 1i3mA01 |
|
|
|
|
|
Unbound | Analogue:UD1 | Unbound | |
| 1i3mB01 |
|
|
|
|
|
Unbound | Analogue:UD1 | Unbound | |
| 1i3nA01 |
|
|
|
|
|
Unbound | Analogue:UD1 | Unbound | |
| 1i3nB01 |
|
|
|
|
|
Unbound | Analogue:UD2 | Unbound | |
| 1a9yA02 |
|
|
|
|
|
Bound:NAD | Unbound | Unbound | |
| 1a9zA02 |
|
|
|
|
|
Bound:NAD | Unbound | Unbound | |
| 1kvqA02 |
|
|
|
|
|
Bound:NAD | Unbound | Unbound | |
| 1kvrA02 |
|
|
|
|
|
Bound:NAD | Unbound | Unbound | |
| 1kvsA02 |
|
|
|
|
|
Bound:NAD | Unbound | Unbound | |
| 1kvtA02 |
|
|
|
|
|
Bound:NAD | Unbound | Unbound | |
| 1kvuA02 |
|
|
|
|
|
Bound:NAD | Unbound | Unbound | |
| 1lrjA02 |
|
|
|
|
|
Bound:NAD | Unbound | Unbound | |
| 1lrkA02 |
|
|
|
|
|
Bound:NAD | Unbound | Unbound | |
| 1lrlA02 |
|
|
|
|
|
Bound:NAD | Unbound | Unbound | |
| 1nahA02 |
|
|
|
|
|
Bound:NAD | Unbound | Unbound | |
| 1naiA02 |
|
|
|
|
|
Bound:NAD | Unbound | Unbound | |
| 1udaA02 |
|
|
|
|
|
Bound:NAD | Unbound | Unbound | |
| 1udbA02 |
|
|
|
|
|
Bound:NAD | Unbound | Unbound | |
| 1udcA02 |
|
|
|
|
|
Bound:NAD | Unbound | Unbound | |
| 1xelA02 |
|
|
|
|
|
Bound:NAD | Unbound | Unbound | |
| 2udpA02 |
|
|
|
|
|
Bound:NAD | Unbound | Unbound | |
| 2udpB02 |
|
|
|
|
|
Bound:NAD | Unbound | Unbound | |
| 3enkA01 |
|
|
|
|
|
Bound:NAD | Unbound | Unbound | |
| 3enkB01 |
|
|
|
|
|
Bound:NAD | Unbound | Unbound | |
| 1gy8A02 |
|
|
|
|
|
Bound:NAD | Unbound | Unbound | |
| 1gy8B02 |
|
|
|
|
|
Bound:NAD | Unbound | Unbound | |
| 1gy8C02 |
|
|
|
|
|
Bound:NAD | Unbound | Unbound | |
| 1gy8D02 |
|
|
|
|
|
Bound:NAD | Unbound | Unbound | |
| 2cnbA02 |
|
|
|
|
|
Bound:NAD | Unbound | Unbound | |
| 2cnbB02 |
|
|
|
|
|
Bound:NAD | Unbound | Unbound | |
| 2cnbC02 |
|
|
|
|
|
Bound:NAD | Unbound | Unbound | |
| 2cnbD02 |
|
|
|
|
|
Bound:NAD | Unbound | Unbound | |
| 1ek5A02 |
|
|
|
|
|
Bound:NAD | Unbound | Unbound | |
| 1ek6A02 |
|
|
|
|
|
Analogue:NAI | Unbound | Unbound | |
| 1ek6B02 |
|
|
|
|
|
Analogue:NAI | Unbound | Unbound | |
| 1hzjA02 |
|
|
|
|
|
Bound:NAD | Unbound | Unbound | |
| 1hzjB02 |
|
|
|
|
|
Bound:NAD | Unbound | Unbound | |
| 1i3kA02 |
|
|
|
|
|
Bound:NAD | Unbound | Unbound | |
| 1i3kB02 |
|
|
|
|
|
Bound:NAD | Unbound | Unbound | |
| 1i3lA02 |
|
|
|
|
|
Bound:NAD | Unbound | Unbound | |
| 1i3lB02 |
|
|
|
|
|
Bound:NAD | Unbound | Unbound | |
| 1i3mA02 |
|
|
|
|
|
Bound:NAD | Unbound | Unbound | |
| 1i3mB02 |
|
|
|
|
|
Bound:NAD | Unbound | Unbound | |
| 1i3nA02 |
|
|
|
|
|
Bound:NAD | Unbound | Unbound | |
| 1i3nB02 |
|
|
|
|
|
Bound:NAD | Unbound | Unbound | |
| Reference for Active-site residues | ||
|---|---|---|
| resource | references | E.C. |
| literature [22], [31], [35] | ||
| Active-site residues | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| PDB | Catalytic residues | Cofactor-binding residues | Modified residues | Main-chain involved in catalysis | Comment | |||||
| 1a9yA01 |
|
|
|
|
|
|||||
| 1a9zA01 |
|
|
|
|
|
|||||
| 1kvqA01 |
|
|
|
|
|
|||||
| 1kvrA01 |
|
|
|
|
|
|||||
| 1kvsA01 |
|
|
|
|
|
|||||
| 1kvtA01 |
|
|
|
|
|
|||||
| 1kvuA01 |
|
|
|
|
|
|||||
| 1lrjA01 |
|
|
|
|
|
|||||
| 1lrkA01 |
|
|
|
|
|
mutant Y299C | ||||
| 1lrlA01 |
|
|
|
|
|
mutant Y299C | ||||
| 1nahA01 |
|
|
|
|
|
|||||
| 1naiA01 |
|
|
|
|
|
|||||
| 1udaA01 |
|
|
|
|
|
|||||
| 1udbA01 |
|
|
|
|
|
|||||
| 1udcA01 |
|
|
|
|
|
|||||
| 1xelA01 |
|
|
|
|
|
|||||
| 2udpA01 |
|
|
|
|
|
|||||
| 2udpB01 |
|
|
|
|
|
|||||
| 3enkA02 |
|
|
|
|
|
|||||
| 3enkB02 |
|
|
|
|
|
|||||
| 1gy8A01 |
|
|
|
|
|
|||||
| 1gy8B01 |
|
|
|
|
|
|||||
| 1gy8C01 |
|
|
|
|
|
|||||
| 1gy8D01 |
|
|
|
|
|
|||||
| 2cnbA01 |
|
|
|
|
|
|||||
| 2cnbB01 |
|
|
|
|
|
|||||
| 2cnbC01 |
|
|
|
|
|
|||||
| 2cnbD01 |
|
|
|
|
|
|||||
| 1ek5A01 |
|
|
|
|
|
|||||
| 1ek6A01 |
|
|
|
|
|
|||||
| 1ek6B01 |
|
|
|
|
|
|||||
| 1hzjA01 |
|
|
|
|
|
|||||
| 1hzjB01 |
|
|
|
|
|
|||||
| 1i3kA01 |
|
|
|
|
|
|||||
| 1i3kB01 |
|
|
|
|
|
|||||
| 1i3lA01 |
|
|
|
|
|
|||||
| 1i3lB01 |
|
|
|
|
|
|||||
| 1i3mA01 |
|
|
|
|
|
|||||
| 1i3mB01 |
|
|
|
|
|
|||||
| 1i3nA01 |
|
|
|
|
|
|||||
| 1i3nB01 |
|
|
|
|
|
|||||
| 1a9yA02 |
|
|
|
|
|
; ;LYS 153 | mutant S124A, Y149F | |||
| 1a9zA02 |
|
|
|
|
|
; ;LYS 153 | mutant S124A, Y149F | |||
| 1kvqA02 |
|
|
|
|
|
;TYR 149;LYS 153 | mutant S124A | |||
| 1kvrA02 |
|
|
|
|
|
;TYR 149;LYS 153 | mutant S124A | |||
| 1kvsA02 |
|
|
|
|
|
;TYR 149;LYS 153 | mutant S124T | |||
| 1kvtA02 |
|
|
|
|
|
;TYR 149;LYS 153 | mutant S124V | |||
| 1kvuA02 |
|
|
|
|
|
SER 124;;LYS 153 | mutant Y149F | |||
| 1lrjA02 |
|
|
|
|
|
SER 124;TYR 149;LYS 153 | ||||
| 1lrkA02 |
|
|
|
|
|
SER 124;TYR 149;LYS 153 | ||||
| 1lrlA02 |
|
|
|
|
|
SER 124;TYR 149;LYS 153 | ||||
| 1nahA02 |
|
|
|
|
|
SER 124;TYR 149;LYS 153 | ||||
| 1naiA02 |
|
|
|
|
|
SER 124;TYR 149;LYS 153 | ||||
| 1udaA02 |
|
|
|
|
|
SER 124;TYR 149;LYS 153 | ||||
| 1udbA02 |
|
|
|
|
|
SER 124;TYR 149;LYS 153 | ||||
| 1udcA02 |
|
|
|
|
|
SER 124;TYR 149;LYS 153 | ||||
| 1xelA02 |
|
|
|
|
|
SER 124;TYR 149;LYS 153 | ||||
| 2udpA02 |
|
|
|
|
|
SER 124;TYR 149;LYS 153 | ||||
| 2udpB02 |
|
|
|
|
|
SER 124;TYR 149;LYS 153 | ||||
| 3enkA01 |
|
|
|
|
|
SER 128;TYR 152;LYS 156 | ||||
| 3enkB01 |
|
|
|
|
|
SER 128;TYR 152;LYS 156 | ||||
| 1gy8A02 |
|
|
|
|
|
SER 142;TYR 173;LYS 177 | ||||
| 1gy8B02 |
|
|
|
|
|
SER 142;TYR 173;LYS 177 | ||||
| 1gy8C02 |
|
|
|
|
|
SER 142;TYR 173;LYS 177 | ||||
| 1gy8D02 |
|
|
|
|
|
SER 142;TYR 173;LYS 177 | ||||
| 2cnbA02 |
|
|
|
|
|
SER 142;TYR 173;LYS 177 | ||||
| 2cnbB02 |
|
|
|
|
|
SER 142;TYR 173;LYS 177 | ||||
| 2cnbC02 |
|
|
|
|
|
SER 142;TYR 173;LYS 177 | ||||
| 2cnbD02 |
|
|
|
|
|
SER 142;TYR 173;LYS 177 | ||||
| 1ek5A02 |
|
|
|
|
|
SER 132;TYR 157;LYS 161 | ||||
| 1ek6A02 |
|
|
|
|
|
SER 132;TYR 157;LYS 161 | ||||
| 1ek6B02 |
|
|
|
|
|
SER 132;TYR 157;LYS 161 | ||||
| 1hzjA02 |
|
|
|
|
|
SER 132;TYR 157;LYS 161 | ||||
| 1hzjB02 |
|
|
|
|
|
SER 132;TYR 157;LYS 161 | ||||
| 1i3kA02 |
|
|
|
|
|
SER 132;TYR 157;LYS 161 | mutant V94M | |||
| 1i3kB02 |
|
|
|
|
|
SER 132;TYR 157;LYS 161 | mutant V94M | |||
| 1i3lA02 |
|
|
|
|
|
SER 132;TYR 157;LYS 161 | mutant V94M | |||
| 1i3lB02 |
|
|
|
|
|
SER 132;TYR 157;LYS 161 | mutant V94M | |||
| 1i3mA02 |
|
|
|
|
|
SER 132;TYR 157;LYS 161 | mutant V94M | |||
| 1i3mB02 |
|
|
|
|
|
SER 132;TYR 157;LYS 161 | mutant V94M | |||
| 1i3nA02 |
|
|
|
|
|
SER 132;TYR 157;LYS 161 | mutant V94M | |||
| 1i3nB02 |
|
|
|
|
|
SER 132;TYR 157;LYS 161 | mutant V94M | |||
| References for Catalytic Mechanism | ||
|---|---|---|
| References | Sections | No. of steps in catalysis |
|
[3]
|
Fig.2, p.135-136 | |
|
[6]
|
Fig.2, p.373 | |
|
[7]
|
p.13227-13230 | |
|
[8]
|
p.13235-13236 | |
|
[11]
|
p.5139-5143 | |
|
[12]
|
Fig.10, p.2156-2159 | |
|
[13]
|
Scheme 1, Scheme 2, Scheme 3, p.10682-10683 | |
|
[14]
|
p.10694-10695 | |
|
[15]
|
Scheme 1, p.6294-6295, p.6302-6303 | |
|
[18]
|
Scheme 1, p.11474-11477 | |
|
[20]
|
Scheme 1, p.5697-5700 | |
|
[22]
|
Scheme 1, Fig.4, p.6703-6705 | |
|
[23]
|
Scheme 1, p.9187-9188 | |
|
[24]
|
Scheme 1, Fig.6, p.11284-11286 | |
|
[29]
|
p.250 | |
|
[30]
|
Scheme 1 | |
|
[31]
|
p.177-179 | |
|
[32]
|
Fig.2, p.13371 | |
|
[33]
|
Scheme 2, p.3065-3066 | |
|
[35]
|
p.832-833 | |
| References | |
|---|---|
| [1] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 3061645 |
| Journal | Carbohydr Res |
| Year | 1988 |
| Volume | 179 |
| Pages | 289-99 |
| Authors | Kaca W, de Jongh-Leuvenink J, Zahringer U, Rietschel ET, Brade H, Verhoef J, Sinnwell V |
| Title | Isolation and chemical analysis of 7-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-L-glycero-D-manno-heptose as a constituent of the lipopolysaccharides of the UDP-galactose epimerase-less mutant J-5 of Escherichia coli and Vibrio cholerae. |
| Related PDB | |
| Related UniProtKB | |
| [2] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2659075 |
| Journal | Biochemistry |
| Year | 1989 |
| Volume | 28 |
| Pages | 2645-54 |
| Authors | Konopka JM, Halkides CJ, Vanhooke JL, Gorenstein DG, Frey PA |
| Title |
UDP-galactose 4-epimerase. |
| Related PDB | |
| Related UniProtKB | |
| [3] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2008433 |
| Journal | Proteins |
| Year | 1991 |
| Volume | 9 |
| Pages | 135-42 |
| Authors | Bauer AJ, Rayment I, Frey PA, Holden HM |
| Title |
The isolation, |
| Related PDB | |
| Related UniProtKB | |
| [4] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1601848 |
| Journal | J Biol Chem |
| Year | 1992 |
| Volume | 267 |
| Pages | 11714-20 |
| Authors | Bhattacharjee H, Bhaduri A |
| Title | Distinct functional roles of two active site thiols in UDPglucose 4-epimerase from Kluyveromyces fragilis. |
| Related PDB | |
| Related UniProtKB | |
| [5] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1297789 |
| Journal | J Nutr Sci Vitaminol (Tokyo) |
| Year | 1992 |
| Volume | Spec No |
| Pages | 461-4 |
| Authors | Frey PA, Bauer AJ, Vanhooke JL, Konopka JM, Rayment I, Holden HM |
| Title | Chemical spectroscopic and crystallographic studies of UDP-galactose 4-epimerase from Escherichia coli. |
| Related PDB | |
| Related UniProtKB | |
| [6] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS). |
| Medline ID | 92253563 |
| PubMed ID | 1579570 |
| Journal | Proteins |
| Year | 1992 |
| Volume | 12 |
| Pages | 372-81 |
| Authors | Bauer AJ, Rayment I, Frey PA, Holden HM |
| Title | The molecular structure of UDP-galactose 4-epimerase from Escherichia coli determined at 2.5 A resolution. |
| Related PDB | 1udp |
| Related UniProtKB | P09147 |
| [7] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8241177 |
| Journal | Biochemistry |
| Year | 1993 |
| Volume | 32 |
| Pages | 13220-30 |
| Authors | Burke JR, Frey PA |
| Title | The importance of binding energy in catalysis of hydride transfer by UDP-galactose 4-epimerase: a 13C and 15N NMR and kinetic study. |
| Related PDB | |
| Related UniProtKB | |
| [8] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8241178 |
| Journal | Biochemistry |
| Year | 1993 |
| Volume | 32 |
| Pages | 13231-6 |
| Authors | Swanson BA, Frey PA |
| Title | Identification of lysine 153 as a functionally important residue in UDP-galactose 4-epimerase from Escherichia coli. |
| Related PDB | |
| Related UniProtKB | |
| [9] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8652544 |
| Journal | Biochemistry |
| Year | 1996 |
| Volume | 35 |
| Pages | 7615-20 |
| Authors | Liu Y, Vanhooke JL, Frey PA |
| Title | UDP-galactose 4-epimerase: NAD+ content and a charge-transfer band associated with the substrate-induced conformational transition. |
| Related PDB | |
| Related UniProtKB | |
| [10] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS). |
| Medline ID | 96180680 |
| PubMed ID | 8611559 |
| Journal | Biochemistry |
| Year | 1996 |
| Volume | 35 |
| Pages | 2557-66 |
| Authors | Thoden JB, Frey PA, Holden HM |
| Title | Crystal structures of the oxidized and reduced forms of UDP-galactose 4-epimerase isolated from Escherichia coli. |
| Related PDB | 1nah 1nai |
| Related UniProtKB | P09147 |
| [11] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 8611497 |
| Journal | Biochemistry |
| Year | 1996 |
| Volume | 35 |
| Pages | 5137-44 |
| Authors | Thoden JB, Frey PA, Holden HM |
| Title | Molecular structure of the NADH/UDP-glucose abortive complex of UDP-galactose 4-epimerase from Escherichia coli: implications for the catalytic mechanism. |
| Related PDB | 1xel |
| Related UniProtKB | |
| [12] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS). |
| Medline ID | 97084800 |
| PubMed ID | 8931134 |
| Journal | Protein Sci |
| Year | 1996 |
| Volume | 5 |
| Pages | 2149-61 |
| Authors | Thoden JB, Frey PA, Holden HM |
| Title | High-resolution X-ray structure of UDP-galactose 4-epimerase complexed with UDP-phenol. |
| Related PDB | 2udp |
| Related UniProtKB | P09147 |
| [13] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 9271498 |
| Journal | Biochemistry |
| Year | 1997 |
| Volume | 36 |
| Pages | 10675-84 |
| Authors | Liu Y, Thoden JB, Kim J, Berger E, Gulick AM, Ruzicka FJ, Holden HM, Frey PA |
| Title | Mechanistic roles of tyrosine 149 and serine 124 in UDP-galactose 4-epimerase from Escherichia coli. |
| Related PDB | 1kvu |
| Related UniProtKB | |
| [14] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF SER-124 MUTANTS. |
| Medline ID | 97419132 |
| PubMed ID | 9271499 |
| Journal | Biochemistry |
| Year | 1997 |
| Volume | 36 |
| Pages | 10685-95 |
| Authors | Thoden JB, Gulick AM, Holden HM |
| Title |
Molecular structures of the S124A, |
| Related PDB | 1kvq 1kvr 1kvs 1kvt |
| Related UniProtKB | P09147 |
| [15] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS). |
| Medline ID | 97317070 |
| PubMed ID | 9174344 |
| Journal | Biochemistry |
| Year | 1997 |
| Volume | 36 |
| Pages | 6294-304 |
| Authors | Thoden JB, Hegeman AD, Wesenberg G, Chapeau MC, Frey PA, Holden HM |
| Title | Structural analysis of UDP-sugar binding to UDP-galactose 4-epimerase from Escherichia coli. |
| Related PDB | 1uda 1udb 1udc |
| Related UniProtKB | P09147 |
| [16] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9119006 |
| Journal | Eur J Biochem |
| Year | 1997 |
| Volume | 244 |
| Pages | 407-13 |
| Authors | Dutta S, Maiti NR, Bhattacharyya D |
| Title | Reversible folding of UDP-galactose 4-epimerase from Escherichia coli. |
| Related PDB | |
| Related UniProtKB | |
| [17] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9224707 |
| Journal | FEBS Lett |
| Year | 1997 |
| Volume | 409 |
| Pages | 449-51 |
| Authors | Nayar S, Bhattacharyya D |
| Title | UDP-galactose 4-epimerase from Escherichia coli: existence of a catalytic monomer. |
| Related PDB | |
| Related UniProtKB | |
| [18] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF MUTANTS. |
| Medline ID | 98376428 |
| PubMed ID | 9708982 |
| Journal | Biochemistry |
| Year | 1998 |
| Volume | 37 |
| Pages | 11469-77 |
| Authors | Thoden JB, Holden HM |
| Title | Dramatic differences in the binding of UDP-galactose and UDP-glucose to UDP-galactose 4-epimerase from Escherichia coli. |
| Related PDB | 1a9y 1a9z |
| Related UniProtKB | P09147 |
| [19] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10329648 |
| Journal | J Biol Chem |
| Year | 1999 |
| Volume | 274 |
| Pages | 14573-8 |
| Authors | Bhattacharyya U, Dhar G, Bhaduri A |
| Title |
An arginine residue is essential for stretching and binding of the substrate on UDP-glucose-4-epimerase from Escherichia coli. |
| Related PDB | |
| Related UniProtKB | |
| [20] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS). |
| Medline ID | 20263487 |
| PubMed ID | 10801319 |
| Journal | Biochemistry |
| Year | 2000 |
| Volume | 39 |
| Pages | 5691-701 |
| Authors | Thoden JB, Wohlers TM, Fridovich-Keil JL, Holden HM |
| Title | Crystallographic evidence for Tyr 157 functioning as the active site base in human UDP-galactose 4-epimerase. |
| Related PDB | 1ek5 1ek6 |
| Related UniProtKB | Q14376 |
| [21] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11437350 |
| Journal | Arch Biochem Biophys |
| Year | 2001 |
| Volume | 391 |
| Pages | 188-96 |
| Authors | Barat B, Bhattacharyya D |
| Title | UDP-galactose 4-epimerase from Escherichia coli: formation of catalytic site during reversible folding. |
| Related PDB | |
| Related UniProtKB | |
| [22] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11380265 |
| Journal | Biochemistry |
| Year | 2001 |
| Volume | 40 |
| Pages | 6699-705 |
| Authors | Berger E, Arabshahi A, Wei Y, Schilling JF, Frey PA |
| Title | Acid-base catalysis by UDP-galactose 4-epimerase: correlations of kinetically measured acid dissociation constants with thermodynamic values for tyrosine 149. |
| Related PDB | |
| Related UniProtKB | |
| [23] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11478886 |
| Journal | Biochemistry |
| Year | 2001 |
| Volume | 40 |
| Pages | 9187-95 |
| Authors | Gerratana B, Cleland WW, Frey PA |
| Title |
Mechanistic roles of Thr134, |
| Related PDB | |
| Related UniProtKB | |
| [24] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11551228 |
| Journal | Biochemistry |
| Year | 2001 |
| Volume | 40 |
| Pages | 11279-87 |
| Authors | Wei Y, Lin J, Frey PA |
| Title | 13C NMR analysis of electrostatic interactions between NAD+ and active site residues of UDP-galactose 4-epimerase: implications for the activation induced by uridine nucleotides. |
| Related PDB | |
| Related UniProtKB | |
| [25] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11726498 |
| Journal | EMBO J |
| Year | 2001 |
| Volume | 20 |
| Pages | 6619-26 |
| Authors | Stammers DK, Ren J, Leslie K, Nichols CE, Lamb HK, Cocklin S, Dodds A, Hawkins AR |
| Title | The structure of the negative transcriptional regulator NmrA reveals a structural superfamily which includes the short-chain dehydrogenase/reductases. |
| Related PDB | |
| Related UniProtKB | |
| [26] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF VARIANT EDG MET-94. |
| Medline ID | 21282994 |
| PubMed ID | 11279193 |
| Journal | J Biol Chem |
| Year | 2001 |
| Volume | 276 |
| Pages | 20617-23 |
| Authors | Thoden JB, Wohlers TM, Fridovich-Keil JL, Holden HM |
| Title |
Molecular basis for severe epimerase deficiency galactosemia. |
| Related PDB | 1i3k 1i3l 1i3m 1i3n |
| Related UniProtKB | Q14376 |
| [27] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 11279032 |
| Journal | J Biol Chem |
| Year | 2001 |
| Volume | 276 |
| Pages | 15131-6 |
| Authors | Thoden JB, Wohlers TM, Fridovich-Keil JL, Holden HM |
| Title |
Human UDP-galactose 4-epimerase. |
| Related PDB | 1hzj |
| Related UniProtKB | |
| [28] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 12019271 |
| Journal | J Biol Chem |
| Year | 2002 |
| Volume | 277 |
| Pages | 27528-34 |
| Authors | Thoden JB, Henderson JM, Fridovich-Keil JL, Holden HM |
| Title |
Structural analysis of the Y299C mutant of Escherichia coli UDP-galactose 4-epimerase. |
| Related PDB | 1lrj 1lrk 1lrl |
| Related UniProtKB | |
| [29] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12604210 |
| Journal | Chem Biol Interact |
| Year | 2003 |
| Volume | 143-144 |
| Pages | 247-53 |
| Authors | Oppermann U, Filling C, Hult M, Shafqat N, Wu X, Lindh M, Shafqat J, Nordling E, Kallberg Y, Persson B, Jornvall H |
| Title | Short-chain dehydrogenases/reductases (SDR): the 2002 update. |
| Related PDB | |
| Related UniProtKB | |
| [30] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12642575 |
| Journal | J Biol Chem |
| Year | 2003 |
| Volume | 278 |
| Pages | 20874-81 |
| Authors | Koropatkin NM, Liu HW, Holden HM |
| Title | High resolution x-ray structure of tyvelose epimerase from Salmonella typhi. |
| Related PDB | |
| Related UniProtKB | |
| [31] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 12615316 |
| Journal | Mol Biochem Parasitol |
| Year | 2003 |
| Volume | 126 |
| Pages | 173-80 |
| Authors | Shaw MP, Bond CS, Roper JR, Gourley DG, Ferguson MA, Hunter WN |
| Title | High-resolution crystal structure of Trypanosoma brucei UDP-galactose 4'-epimerase: a potential target for structure-based development of novel trypanocides. |
| Related PDB | 1gy8 |
| Related UniProtKB | |
| [32] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 15491143 |
| Journal | Biochemistry |
| Year | 2004 |
| Volume | 43 |
| Pages | 13370-9 |
| Authors | Gatzeva-Topalova PZ, May AP, Sousa MC |
| Title |
Crystal structure of Escherichia coli ArnA (PmrI) decarboxylase domain. |
| Related PDB | |
| Related UniProtKB | |
| [33] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 15023057 |
| Journal | Biochemistry |
| Year | 2004 |
| Volume | 43 |
| Pages | 3057-67 |
| Authors | Vogan EM, Bellamacina C, He X, Liu HW, Ringe D, Petsko GA |
| Title | Crystal structure at 1.8 A resolution of CDP-D-glucose 4,6-dehydratase from Yersinia pseudotuberculosis. |
| Related PDB | |
| Related UniProtKB | |
| [34] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 15175331 |
| Journal | J Biol Chem |
| Year | 2004 |
| Volume | 279 |
| Pages | 32796-803 |
| Authors | Schulz JM, Watson AL, Sanders R, Ross KL, Thoden JB, Holden HM, Fridovich-Keil JL |
| Title | Determinants of function and substrate specificity in human UDP-galactose 4'-epimerase. |
| Related PDB | |
| Related UniProtKB | |
| [35] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 16946458 |
| Journal | Acta Crystallograph Sect F Struct Biol Cryst Commun |
| Year | 2006 |
| Volume | 62 |
| Pages | 829-34 |
| Authors | Alphey MS, Burton A, Urbaniak MD, Boons GJ, Ferguson MA, Hunter WN |
| Title | Trypanosoma brucei UDP-galactose-4'-epimerase in ternary complex with NAD+ and the substrate analogue UDP-4-deoxy-4-fluoro-alpha-D-galactose. |
| Related PDB | 2cnb |
| Related UniProtKB | |
| Comments |
|---|
|
This enzyme is a distant homologue of the short-chain dehydrogenase/reductase (SDR) superfamily, According to the literature [22], (A) Hydride transfer from C4' atom of substrate to NAD+, This reaction mechanism must be the same as that by Drosophia alcohol dehydrogenase. (X) Rotation or conformation change of the intermediate. (B) Hydride transfer from NADH to C4' atom of the 4-ketose intermediate, This reaction mechanism also must be the same as the reverse reaction by Drosophia alcohol dehydrogenase. |
| Created | Updated |
|---|---|
| 2005-06-07 | 2011-07-05 |