DB code: M00193
| RLCP classification | 1.30.36000.3 : Hydrolysis | |
|---|---|---|
| CATH domain | 2.60.40.10 : Immunoglobulin-like | |
| 3.20.20.80 : TIM Barrel | Catalytic domain | |
| 1.-.-.- : | ||
| 2.60.40.1180 : Immunoglobulin-like | ||
| E.C. | 3.2.1.135 | |
| CSA | 1bvz | |
| M-CSA | 1bvz | |
| MACiE | ||
| CATH domain | Related DB codes (homologues) |
|---|---|
| 2.60.40.10 : Immunoglobulin-like | M00131 T00257 T00005 M00113 M00127 M00132 M00323 M00325 M00327 M00329 M00330 M00331 M00332 T00307 D00166 D00500 M00112 T00063 T00065 T00067 T00245 |
| 2.60.40.1180 : Immunoglobulin-like | M00113 T00307 D00165 D00176 D00664 D00665 D00863 D00864 M00112 M00314 T00057 T00062 T00067 |
| 3.20.20.80 : TIM Barrel | S00202 S00210 S00748 S00906 S00907 S00911 S00912 S00915 M00134 M00160 D00479 S00204 S00205 S00206 S00207 S00203 S00208 S00209 S00211 S00213 S00214 M00113 T00307 D00165 D00166 D00169 D00176 D00501 D00502 D00503 D00844 D00861 D00864 M00026 M00112 M00346 T00057 T00062 T00063 T00066 T00067 |
| Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | CAZy | Pfam |
|---|---|---|---|---|
| Q08751 |
Neopullulanase 2
|
EC
3.2.1.135
Alpha-amylase II TVA II |
CBM34
(Carbohydrate-Binding Module Family 34)
GH13 (Glycoside Hydrolase Family 13) |
PF00128
(Alpha-amylase)
PF02806 (Alpha-amylase_C) PF02903 (Alpha-amylase_N) [Graphical View] |
| P38940 |
Neopullulanase
|
EC
3.2.1.135
|
CBM34
(Carbohydrate-Binding Module Family 34)
GH13 (Glycoside Hydrolase Family 13) |
PF00128
(Alpha-amylase)
PF02903 (Alpha-amylase_N) [Graphical View] |
| KEGG enzyme name |
|---|
|
neopullulanase
pullulanase II |
| UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
|---|---|---|---|---|---|
| Q08751 | NEPU2_THEVU | Hydrolysis of pullulan to panose (6-alpha-D- glucosylmaltose). | Monomer. | Binds 1 calcium ion per subunit. | |
| P38940 | NEPU_BACST | Hydrolysis of pullulan to panose (6-alpha-D- glucosylmaltose). | Dimer. | Binds 1 calcium ion per subunit. |
| KEGG Pathways | Map code | Pathways | E.C. |
|---|
| Compound table | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Cofactors | Substrates | Products | Intermediates | ||||||||
| KEGG-id | C00076 | C00480 | C00001 | C00480 | C00713 | ||||||
| E.C. | |||||||||||
| Compound | Calcium | Pullulan | H2O | Pullulan | Panose | ||||||
| Type | divalent metal (Ca2+, Mg2+) | polysaccharide | H2O | polysaccharide | polysaccharide | ||||||
| ChEBI |
29108 29108 |
15377 15377 |
|||||||||
| PubChem |
271 271 |
22247451 962 22247451 962 |
44630447 44630447 |
||||||||
| 1bvzA01 |
|
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Unbound | Unbound | Unbound | Unbound | ||
| 1bvzB01 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1g1yA01 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1g1yB01 |
|
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Unbound | Unbound | Unbound | Unbound | ||
| 1jf5A01 |
|
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Unbound | Unbound | Unbound | Unbound | ||
| 1jf5B01 |
|
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Unbound | Unbound | Unbound | Unbound | ||
| 1jf6A01 |
|
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Unbound | Unbound | Unbound | Unbound | ||
| 1jf6B01 |
|
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Unbound | Unbound | Unbound | Unbound | ||
| 1ji2A01 |
|
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Unbound | Unbound | Unbound | Unbound | ||
| 1ji2B01 |
|
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Unbound | Unbound | Unbound | Unbound | ||
| 1jibA01 |
|
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Unbound | Unbound | Unbound | Unbound | ||
| 1jibB01 |
|
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Unbound | Unbound | Unbound | Unbound | ||
| 1jl8A01 |
|
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Unbound | Unbound | Unbound | Unbound | ||
| 1jl8B01 |
|
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Unbound | Unbound | Unbound | Unbound | ||
| 1vb9A01 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1vb9B01 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1vfkA01 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1vfkB01 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1vfmA01 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1vfmB01 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1vfoA01 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1vfoB01 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1vfuA01 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1vfuB01 |
|
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Unbound | Unbound | Unbound | Unbound | ||
| 1wzkA01 |
|
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Unbound | Unbound | Unbound | Unbound | ||
| 1wzkB01 |
|
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Unbound | Unbound | Unbound | Unbound | ||
| 1wzlA01 |
|
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Unbound | Unbound | Unbound | Unbound | ||
| 1wzlB01 |
|
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Unbound | Unbound | Unbound | Unbound | ||
| 1wzmA01 |
|
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Unbound | Unbound | Unbound | Unbound | ||
| 1wzmB01 |
|
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Unbound | Unbound | Unbound | Unbound | ||
| 1j0hA01 |
|
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Unbound | Unbound | Unbound | Unbound | ||
| 1j0hB01 |
|
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Unbound | Unbound | Unbound | Unbound | ||
| 1j0iA01 |
|
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Unbound | Unbound | Unbound | Unbound | ||
| 1j0iB01 |
|
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Unbound | Unbound | Unbound | Unbound | ||
| 1j0jA01 |
|
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Unbound | Unbound | Unbound | Unbound | ||
| 1j0jB01 |
|
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Unbound | Unbound | Unbound | Unbound | ||
| 1j0kA01 |
|
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Unbound | Unbound | Unbound | Unbound | ||
| 1j0kB01 |
|
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Unbound | Unbound | Unbound | Unbound | ||
| 1bvzA02 |
|
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Unbound | Unbound | Unbound | Unbound | ||
| 1bvzB02 |
|
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Unbound | Unbound | Unbound | Unbound | ||
| 1g1yA02 |
|
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Unbound | Analogue:BCD | Unbound | Unbound | ||
| 1g1yB02 |
|
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Unbound | Analogue:BCD | Unbound | Unbound | ||
| 1jf5A02 |
|
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Bound:_CA | Unbound | Unbound | Unbound | ||
| 1jf5B02 |
|
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Bound:_CA | Unbound | Unbound | Unbound | ||
| 1jf6A02 |
|
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Bound:_CA | Unbound | Unbound | Unbound | ||
| 1jf6B02 |
|
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Bound:_CA | Unbound | Unbound | Unbound | ||
| 1ji2A02 |
|
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Bound:_CA | Unbound | Unbound | Unbound | ||
| 1ji2B02 |
|
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Bound:_CA | Unbound | Unbound | Unbound | ||
| 1jibA02 |
|
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Unbound | Analogue:MTT | Unbound | Unbound | ||
| 1jibB02 |
|
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Unbound | Analogue:MTT | Unbound | Unbound | ||
| 1jl8A02 |
|
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Unbound | Analogue:BCD | Unbound | Unbound | ||
| 1jl8B02 |
|
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Unbound | Analogue:BCD | Unbound | Unbound | ||
| 1vb9A02 |
|
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Bound:_CA | Analogue:GLC-GLC-GLC-GLC-GLC-GLC | Unbound | Unbound | ||
| 1vb9B02 |
|
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Bound:_CA | Analogue:GLC-GLC-GLC-GLC-GLC-GLC | Unbound | Unbound | ||
| 1vfkA02 |
|
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Bound:_CA | Unbound | Analogue:GLC-GLC | Analogue:GLC | ||
| 1vfkB02 |
|
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Bound:_CA | Unbound | Analogue:GLC-GLC | Analogue:GLC | ||
| 1vfmA02 |
|
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Bound:_CA | Analogue:GLC-GLC-GLC-GLC-GLC-GLC | Unbound | Unbound | ||
| 1vfmB02 |
|
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Bound:_CA | Analogue:GLC-GLC-GLC-GLC-GLC-GLC | Unbound | Unbound | ||
| 1vfoA02 |
|
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Bound:_CA | Analogue:GLC-GLC-GLC-GLC-GLC-GLC-GLC | Unbound | Unbound | ||
| 1vfoB02 |
|
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Bound:_CA | Analogue:GLC-GLC-GLC-GLC-GLC-GLC-GLC | Unbound | Unbound | ||
| 1vfuA02 |
|
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Bound:_CA | Analogue:GLC-GLC-GLC-GLC-GLC-GLC-GLC-GLC | Unbound | Unbound | ||
| 1vfuB02 |
|
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Bound:_CA | Analogue:GLC-GLC-GLC-GLC-GLC-GLC-GLC | Unbound | Unbound | ||
| 1wzkA02 |
|
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Bound:_CA | Unbound | Unbound | Unbound | ||
| 1wzkB02 |
|
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Bound:_CA | Unbound | Unbound | Unbound | ||
| 1wzlA02 |
|
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Bound:_CA | Unbound | Unbound | Unbound | ||
| 1wzlB02 |
|
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Bound:_CA | Unbound | Unbound | Unbound | ||
| 1wzmA02 |
|
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Bound:_CA | Unbound | Unbound | Unbound | ||
| 1wzmB02 |
|
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Bound:_CA | Unbound | Unbound | Unbound | ||
| 1j0hA02 |
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Bound:_CA | Unbound | Unbound | Unbound | ||
| 1j0hB02 |
|
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Bound:_CA | Unbound | Unbound | Unbound | ||
| 1j0iA02 |
|
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Unbound | Unbound | Bound:GLC-GLC-GLC | Unbound | ||
| 1j0iB02 |
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Unbound | Unbound | Bound:GLC-GLC-GLC | Unbound | ||
| 1j0jA02 |
|
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Unbound | Analogue:GLC-GLC-GLC-GLC | Unbound | Unbound | ||
| 1j0jB02 |
|
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Unbound | Analogue:GLC-GLC-GLC-GLC | Unbound | Unbound | ||
| 1j0kA02 |
|
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Unbound | Analogue:GLC-GLC-GLC | Unbound | Unbound | ||
| 1j0kB02 |
|
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Unbound | Analogue:GLC-GLC-GLC | Unbound | Unbound | ||
| 1bvzA03 |
|
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Unbound | Unbound | Unbound | Unbound | ||
| 1bvzB03 |
|
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Unbound | Unbound | Unbound | Unbound | ||
| 1g1yA03 |
|
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Unbound | Unbound | Unbound | Unbound | ||
| 1g1yB03 |
|
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Unbound | Unbound | Unbound | Unbound | ||
| 1jf5A03 |
|
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Unbound | Unbound | Unbound | Unbound | ||
| 1jf5B03 |
|
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Unbound | Unbound | Unbound | Unbound | ||
| 1jf6A03 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1jf6B03 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1ji2A03 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1ji2B03 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1jibA03 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1jibB03 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1jl8A03 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1jl8B03 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1vb9A03 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1vb9B03 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1vfkA03 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1vfkB03 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1vfmA03 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1vfmB03 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1vfoA03 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1vfoB03 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1vfuA03 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1vfuB03 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1wzkA03 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1wzkB03 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1wzlA03 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1wzlB03 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1wzmA03 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1wzmB03 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1j0hA03 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1j0hB03 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1j0iA03 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1j0iB03 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1j0jA03 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1j0jB03 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1j0kA03 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1j0kB03 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1bvzA04 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1bvzB04 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1g1yA04 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1g1yB04 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1jf5A04 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1jf5B04 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1jf6A04 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1jf6B04 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1ji2A04 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1ji2B04 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1jibA04 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1jibB04 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1jl8A04 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1jl8B04 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1vb9A04 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1vb9B04 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1vfkA04 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1vfkB04 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1vfmA04 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1vfmB04 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1vfoA04 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1vfoB04 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1vfuA04 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1vfuB04 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1wzkA04 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1wzkB04 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1wzlA04 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1wzlB04 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1wzmA04 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1wzmB04 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1j0hA04 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1j0hB04 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1j0iA04 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1j0iB04 |
|
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Unbound | Unbound | Unbound | Unbound | ||
| 1j0jA04 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1j0jB04 |
|
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Unbound | Unbound | Unbound | Unbound | ||
| 1j0kA04 |
|
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Unbound | Unbound | Unbound | Unbound | ||
| 1j0kB04 |
|
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|
Unbound | Unbound | Unbound | Unbound | ||
| Reference for Active-site residues | ||
|---|---|---|
| resource | references | E.C. |
| Swiss-prot;Q08751, P38940 & literature [9], [11], [20] | ||
| Active-site residues | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| PDB | Catalytic residues | Cofactor-binding residues | Modified residues | Main-chain involved in catalysis | Comment | |||||
| 1bvzA01 |
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| 1bvzB01 |
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| 1g1yA01 |
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| 1g1yB01 |
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| 1jf5A01 |
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| 1jf5B01 |
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| 1jf6A01 |
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| 1jf6B01 |
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| 1ji2A01 |
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| 1ji2B01 |
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| 1jibA01 |
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| 1jibB01 |
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| 1jl8A01 |
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| 1jl8B01 |
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| 1vb9A01 |
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| 1vb9B01 |
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| 1vfkA01 |
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| 1vfkB01 |
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| 1vfmA01 |
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| 1vfmB01 |
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| 1vfoA01 |
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| 1vfoB01 |
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| 1vfuA01 |
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| 1vfuB01 |
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|
|||||
| 1wzkA01 |
|
|
|
|
|
|||||
| 1wzkB01 |
|
|
|
|
|
|||||
| 1wzlA01 |
|
|
|
|
|
|||||
| 1wzlB01 |
|
|
|
|
|
|||||
| 1wzmA01 |
|
|
|
|
|
|||||
| 1wzmB01 |
|
|
|
|
|
|||||
| 1j0hA01 |
|
|
|
|
|
|||||
| 1j0hB01 |
|
|
|
|
|
|||||
| 1j0iA01 |
|
|
|
|
|
|||||
| 1j0iB01 |
|
|
|
|
|
|||||
| 1j0jA01 |
|
|
|
|
|
|||||
| 1j0jB01 |
|
|
|
|
|
|||||
| 1j0kA01 |
|
|
|
|
|
|||||
| 1j0kB01 |
|
|
|
|
|
|||||
| 1bvzA02 |
|
|
|
|
|
ASP 325;GLU 354;ASP 421 | ASN 143;ASP 145;ASN 148;ASP 149;GLY 169;ASP 171(Calcium binding) | |||
| 1bvzB02 |
|
|
|
|
|
ASP 325;GLU 354;ASP 421 | ASN 143;ASP 145;ASN 148;ASP 149;GLY 169;ASP 171(Calcium binding) | |||
| 1g1yA02 |
|
|
|
|
|
ASP 325;;ASP 421 | ASN 143;ASP 145;ASN 148;ASP 149;GLY 169;ASP 171(Calcium binding) | mutant E354A | ||
| 1g1yB02 |
|
|
|
|
|
ASP 325;;ASP 421 | ASN 143;ASP 145;ASN 148;ASP 149;GLY 169;ASP 171(Calcium binding) | mutant E354A | ||
| 1jf5A02 |
|
|
|
|
|
ASP 325;GLU 354;ASP 421 | ASN 143;ASP 145;ASN 148;ASP 149;GLY 169;ASP 171(Calcium binding) | mutant F286A | ||
| 1jf5B02 |
|
|
|
|
|
ASP 325;GLU 354;ASP 421 | ASN 143;ASP 145;ASN 148;ASP 149;GLY 169;ASP 171(Calcium binding) | mutant F286A | ||
| 1jf6A02 |
|
|
|
|
|
ASP 325;GLU 354;ASP 421 | ASN 143;ASP 145;ASN 148;ASP 149;GLY 169;ASP 171(Calcium binding) | mutant F286Y | ||
| 1jf6B02 |
|
|
|
|
|
ASP 325;GLU 354;ASP 421 | ASN 143;ASP 145;ASN 148;ASP 149;GLY 169;ASP 171(Calcium binding) | mutant F286Y | ||
| 1ji2A02 |
|
|
|
|
|
ASP 325;GLU 354;ASP 421 | ASN 143;ASP 145;ASN 148;ASP 149;GLY 169;ASP 171(Calcium binding) | |||
| 1ji2B02 |
|
|
|
|
|
ASP 325;GLU 354;ASP 421 | ASN 143;ASP 145;ASN 148;ASP 149;GLY 169;ASP 171(Calcium binding) | |||
| 1jibA02 |
|
|
|
|
|
;GLU 354;ASP 421 | ASN 143;ASP 145;ASN 148;ASP 149;GLY 169;ASP 171(Calcium binding) | mutant D325N | ||
| 1jibB02 |
|
|
|
|
|
;GLU 354;ASP 421 | ASN 143;ASP 145;ASN 148;ASP 149;GLY 169;ASP 171(Calcium binding) | mutant D325N | ||
| 1jl8A02 |
|
|
|
|
|
;GLU 354;ASP 421 | ASN 143;ASP 145;ASN 148;ASP 149;GLY 169;ASP 171(Calcium binding) | mutant D325N | ||
| 1jl8B02 |
|
|
|
|
|
;GLU 354;ASP 421 | ASN 143;ASP 145;ASN 148;ASP 149;GLY 169;ASP 171(Calcium binding) | mutant D325N | ||
| 1vb9A02 |
|
|
|
|
|
;GLU 354;ASP 421 | ASN 143;ASP 145;ASN 148;ASP 149;GLY 169;ASP 171(Calcium binding) | mutant D325N | ||
| 1vb9B02 |
|
|
|
|
|
;GLU 354;ASP 421 | ASN 143;ASP 145;ASN 148;ASP 149;GLY 169;ASP 171(Calcium binding) | mutant D325N | ||
| 1vfkA02 |
|
|
|
|
|
ASP 325;GLU 354;ASP 421 | ASN 143;ASP 145;ASN 148;ASP 149;GLY 169;ASP 171(Calcium binding) | |||
| 1vfkB02 |
|
|
|
|
|
ASP 325;GLU 354;ASP 421 | ASN 143;ASP 145;ASN 148;ASP 149;GLY 169;ASP 171(Calcium binding) | |||
| 1vfmA02 |
|
|
|
|
|
;GLU 354; | ASN 143;ASP 145;ASN 148;ASP 149;GLY 169;ASP 171(Calcium binding) | mutant D325N, D421N | ||
| 1vfmB02 |
|
|
|
|
|
;GLU 354; | ASN 143;ASP 145;ASN 148;ASP 149;GLY 169;ASP 171(Calcium binding) | mutant D325N, D421N | ||
| 1vfoA02 |
|
|
|
|
|
;GLU 354; | ASN 143;ASP 145;ASN 148;ASP 149;GLY 169;ASP 171(Calcium binding) | mutant D325N, D421N | ||
| 1vfoB02 |
|
|
|
|
|
;GLU 354; | ASN 143;ASP 145;ASN 148;ASP 149;GLY 169;ASP 171(Calcium binding) | mutant D325N, D421N | ||
| 1vfuA02 |
|
|
|
|
|
;GLU 354; | ASN 143;ASP 145;ASN 148;ASP 149;GLY 169;ASP 171(Calcium binding) | mutant D325N, D421N | ||
| 1vfuB02 |
|
|
|
|
|
;GLU 354; | ASN 143;ASP 145;ASN 148;ASP 149;GLY 169;ASP 171(Calcium binding) | mutant D325N, D421N | ||
| 1wzkA02 |
|
|
|
|
|
ASP 325;GLU 354;ASP 421 | ASN 143;ASP 145;ASN 148;ASP 149;GLY 169;ASP 171(Calcium binding) | mutant D465N | ||
| 1wzkB02 |
|
|
|
|
|
ASP 325;GLU 354;ASP 421 | ASN 143;ASP 145;ASN 148;ASP 149;GLY 169;ASP 171(Calcium binding) | mutant D465N | ||
| 1wzlA02 |
|
|
|
|
|
ASP 325;GLU 354;ASP 421 | ASN 143;ASP 145;ASN 148;ASP 149;GLY 169;ASP 171(Calcium binding) | mutant R469L | ||
| 1wzlB02 |
|
|
|
|
|
ASP 325;GLU 354;ASP 421 | ASN 143;ASP 145;ASN 148;ASP 149;GLY 169;ASP 171(Calcium binding) | mutant R469L | ||
| 1wzmA02 |
|
|
|
|
|
ASP 325;GLU 354;ASP 421 | ASN 143;ASP 145;ASN 148;ASP 149;GLY 169;ASP 171(Calcium binding) | mutant R469K | ||
| 1wzmB02 |
|
|
|
|
|
ASP 325;GLU 354;ASP 421 | ASN 143;ASP 145;ASN 148;ASP 149;GLY 169;ASP 171(Calcium binding) | mutant R469K | ||
| 1j0hA02 |
|
|
|
|
|
ASP 328;GLU 357;ASP 424 | ASN 147;ASN 149;;SER 153;GLY 172;ASP 174(Calcium binding) | |||
| 1j0hB02 |
|
|
|
|
|
ASP 328;GLU 357;ASP 424 | ASN 147;ASN 149;;SER 153;GLY 172;ASP 174(Calcium binding) | |||
| 1j0iA02 |
|
|
|
|
|
ASP 328;GLU 357;ASP 424 | ASN 147;ASN 149;;SER 153;GLY 172;ASP 174(Calcium binding) | |||
| 1j0iB02 |
|
|
|
|
|
ASP 328;GLU 357;ASP 424 | ASN 147;ASN 149;;SER 153;GLY 172;ASP 174(Calcium binding) | |||
| 1j0jA02 |
|
|
|
|
|
ASP 328;;ASP 424 | ASN 147;ASN 149;;SER 153;GLY 172;ASP 174(Calcium binding) | mutantE357Q | ||
| 1j0jB02 |
|
|
|
|
|
ASP 328;;ASP 424 | ASN 147;ASN 149;;SER 153;GLY 172;ASP 174(Calcium binding) | mutantE357Q | ||
| 1j0kA02 |
|
|
|
|
|
ASP 328;;ASP 424 | ASN 147;ASN 149;;SER 153;GLY 172;ASP 174(Calcium binding) | mutantE357Q | ||
| 1j0kB02 |
|
|
|
|
|
ASP 328;;ASP 424 | ASN 147;ASN 149;;SER 153;GLY 172;ASP 174(Calcium binding) | mutantE357Q | ||
| 1bvzA03 |
|
|
|
|
|
|||||
| 1bvzB03 |
|
|
|
|
|
|||||
| 1g1yA03 |
|
|
|
|
|
|||||
| 1g1yB03 |
|
|
|
|
|
|||||
| 1jf5A03 |
|
|
|
|
|
|||||
| 1jf5B03 |
|
|
|
|
|
|||||
| 1jf6A03 |
|
|
|
|
|
|||||
| 1jf6B03 |
|
|
|
|
|
|||||
| 1ji2A03 |
|
|
|
|
|
|||||
| 1ji2B03 |
|
|
|
|
|
|||||
| 1jibA03 |
|
|
|
|
|
|||||
| 1jibB03 |
|
|
|
|
|
|||||
| 1jl8A03 |
|
|
|
|
|
|||||
| 1jl8B03 |
|
|
|
|
|
|||||
| 1vb9A03 |
|
|
|
|
|
|||||
| 1vb9B03 |
|
|
|
|
|
|||||
| 1vfkA03 |
|
|
|
|
|
|||||
| 1vfkB03 |
|
|
|
|
|
|||||
| 1vfmA03 |
|
|
|
|
|
|||||
| 1vfmB03 |
|
|
|
|
|
|||||
| 1vfoA03 |
|
|
|
|
|
|||||
| 1vfoB03 |
|
|
|
|
|
|||||
| 1vfuA03 |
|
|
|
|
|
|||||
| 1vfuB03 |
|
|
|
|
|
|||||
| 1wzkA03 |
|
|
|
|
|
|||||
| 1wzkB03 |
|
|
|
|
|
|||||
| 1wzlA03 |
|
|
|
|
|
|||||
| 1wzlB03 |
|
|
|
|
|
|||||
| 1wzmA03 |
|
|
|
|
|
|||||
| 1wzmB03 |
|
|
|
|
|
|||||
| 1j0hA03 |
|
|
|
|
|
|||||
| 1j0hB03 |
|
|
|
|
|
|||||
| 1j0iA03 |
|
|
|
|
|
|||||
| 1j0iB03 |
|
|
|
|
|
|||||
| 1j0jA03 |
|
|
|
|
|
|||||
| 1j0jB03 |
|
|
|
|
|
|||||
| 1j0kA03 |
|
|
|
|
|
|||||
| 1j0kB03 |
|
|
|
|
|
|||||
| 1bvzA04 |
|
|
|
|
|
|||||
| 1bvzB04 |
|
|
|
|
|
|||||
| 1g1yA04 |
|
|
|
|
|
|||||
| 1g1yB04 |
|
|
|
|
|
|||||
| 1jf5A04 |
|
|
|
|
|
|||||
| 1jf5B04 |
|
|
|
|
|
|||||
| 1jf6A04 |
|
|
|
|
|
|||||
| 1jf6B04 |
|
|
|
|
|
|||||
| 1ji2A04 |
|
|
|
|
|
|||||
| 1ji2B04 |
|
|
|
|
|
|||||
| 1jibA04 |
|
|
|
|
|
|||||
| 1jibB04 |
|
|
|
|
|
|||||
| 1jl8A04 |
|
|
|
|
|
|||||
| 1jl8B04 |
|
|
|
|
|
|||||
| 1vb9A04 |
|
|
|
|
|
|||||
| 1vb9B04 |
|
|
|
|
|
|||||
| 1vfkA04 |
|
|
|
|
|
|||||
| 1vfkB04 |
|
|
|
|
|
|||||
| 1vfmA04 |
|
|
|
|
|
|||||
| 1vfmB04 |
|
|
|
|
|
|||||
| 1vfoA04 |
|
|
|
|
|
|||||
| 1vfoB04 |
|
|
|
|
|
|||||
| 1vfuA04 |
|
|
|
|
|
|||||
| 1vfuB04 |
|
|
|
|
|
|||||
| 1wzkA04 |
|
|
|
|
|
|||||
| 1wzkB04 |
|
|
|
|
|
|||||
| 1wzlA04 |
|
|
|
|
|
|||||
| 1wzlB04 |
|
|
|
|
|
|||||
| 1wzmA04 |
|
|
|
|
|
|||||
| 1wzmB04 |
|
|
|
|
|
|||||
| 1j0hA04 |
|
|
|
|
|
|||||
| 1j0hB04 |
|
|
|
|
|
|||||
| 1j0iA04 |
|
|
|
|
|
|||||
| 1j0iB04 |
|
|
|
|
|
|||||
| 1j0jA04 |
|
|
|
|
|
|||||
| 1j0jB04 |
|
|
|
|
|
|||||
| 1j0kA04 |
|
|
|
|
|
|||||
| 1j0kB04 |
|
|
|
|
|
|||||
| References for Catalytic Mechanism | ||
|---|---|---|
| References | Sections | No. of steps in catalysis |
|
[5]
|
p.146-150 | |
|
[10]
|
Fig.2, p.170 | |
|
[12]
|
Fig.2 | |
|
[22]
|
FIG.5, p.31038 | |
| References | |
|---|---|
| [1] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1388153 |
| Journal | J Biol Chem |
| Year | 1992 |
| Volume | 267 |
| Pages | 18447-52 |
| Authors | Takata H, Kuriki T, Okada S, Takesada Y, Iizuka M, Minamiura N, Imanaka T |
| Title |
Action of neopullulanase. |
| Related PDB | |
| Related UniProtKB | |
| [2] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8136030 |
| Journal | J Protein Chem |
| Year | 1993 |
| Volume | 12 |
| Pages | 791-805 |
| Authors | Jespersen HM, MacGregor EA, Henrissat B, Sierks MR, Svensson B |
| Title | Starch- and glycogen-debranching and branching enzymes: prediction of structural features of the catalytic (beta/alpha)8-barrel domain and evolutionary relationship to other amylolytic enzymes. |
| Related PDB | |
| Related UniProtKB | |
| [3] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8087809 |
| Journal | Carbohydr Res |
| Year | 1994 |
| Volume | 261 |
| Pages | 157-62 |
| Authors | Tonozuka T, Sakai H, Ohta T, Sakano Y |
| Title | A convenient enzymatic synthesis of 4(2)-alpha-isomaltosylisomaltose using Thermoactinomyces vulgaris R-47 alpha-amylase II (TVA II). |
| Related PDB | |
| Related UniProtKB | |
| [4] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7822101 |
| Journal | Int J Pept Protein Res |
| Year | 1994 |
| Volume | 44 |
| Pages | 245-52 |
| Authors | Hansen G, Heese O, Hohne WE, Hofemeister B |
| Title |
alpha-Amylases from Thermoactinomyces vulgaris: characteristics, |
| Related PDB | |
| Related UniProtKB | |
| [5] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8018865 |
| Journal | Plant Mol Biol |
| Year | 1994 |
| Volume | 25 |
| Pages | 141-57 |
| Authors | Svensson B |
| Title |
Protein engineering in the alpha-amylase family: catalytic mechanism, |
| Related PDB | |
| Related UniProtKB | |
| [6] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8695646 |
| Journal | Biochim Biophys Acta |
| Year | 1996 |
| Volume | 1295 |
| Pages | 195-200 |
| Authors | Lamminmaki U, Vihinen M |
| Title | Structural consequences of neopullulanase mutations. |
| Related PDB | |
| Related UniProtKB | |
| [7] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8663322 |
| Journal | J Biol Chem |
| Year | 1996 |
| Volume | 271 |
| Pages | 17321-9 |
| Authors | Kuriki T, Kaneko H, Yanase M, Takata H, Shimada J, Handa S, Takada T, Umeyama H, Okada S |
| Title | Controlling substrate preference and transglycosylation activity of neopullulanase by manipulating steric constraint and hydrophobicity in active center. |
| Related PDB | |
| Related UniProtKB | |
| [8] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10403384 |
| Journal | FEBS Lett |
| Year | 1999 |
| Volume | 453 |
| Pages | 100-6 |
| Authors | Saab-Rincon G, del-Rio G, Santamaria RI, Lopez-Munguia A, Soberon X |
| Title | Introducing transglycosylation activity in a liquefying alpha-amylase. |
| Related PDB | |
| Related UniProtKB | |
| [9] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS). |
| Medline ID | 99241045 |
| PubMed ID | 10222200 |
| Journal | J Mol Biol |
| Year | 1999 |
| Volume | 287 |
| Pages | 907-21 |
| Authors | Kamitori S, Kondo S, Okuyama K, Yokota T, Shimura Y, Tonozuka T, Sakano Y |
| Title | Crystal structure of Thermoactinomyces vulgaris R-47 alpha-amylase II (TVAII) hydrolyzing cyclodextrins and pullulan at 2.6 A resolution. |
| Related PDB | 1bvz |
| Related UniProtKB | Q08751 |
| [10] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10825529 |
| Journal | Biochim Biophys Acta |
| Year | 2000 |
| Volume | 1478 |
| Pages | 165-85 |
| Authors | Park KH, Kim TJ, Cheong TK, Kim JW, Oh BH, Svensson B |
| Title |
Structure, |
| Related PDB | |
| Related UniProtKB | |
| [11] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11210138 |
| Journal | Biosci Biotechnol Biochem |
| Year | 2000 |
| Volume | 64 |
| Pages | 2692-5 |
| Authors | Ichikawa K, Tonozuka T, Yokota T, Shimura Y, Sakano Y |
| Title | Analysis of catalytic residues of Thermoactinomyces vulgaris R-47 alpha-amylase II (TVA II) by site-directed mutagenesis. |
| Related PDB | |
| Related UniProtKB | |
| [12] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11257505 |
| Journal | Biochim Biophys Acta |
| Year | 2001 |
| Volume | 1546 |
| Pages | 1-20 |
| Authors | MacGregor EA, Janecek S, Svensson B |
| Title | Relationship of sequence and structure to specificity in the alpha-amylase family of enzymes. |
| Related PDB | |
| Related UniProtKB | |
| [13] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 11330677 |
| Journal | Biosci Biotechnol Biochem |
| Year | 2001 |
| Volume | 65 |
| Pages | 619-26 |
| Authors | Yokota T, Tonozuka T, Shimura Y, Ichikawa K, Kamitori S, Sakano Y |
| Title | Structures of Thermoactinomyces vulgaris R-47 alpha-amylase II complexed with substrate analogues. |
| Related PDB | 1jib 1jl8 |
| Related UniProtKB | |
| [14] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 11527532 |
| Journal | Carbohydr Res |
| Year | 2001 |
| Volume | 334 |
| Pages | 309-13 |
| Authors | Ohtaki A, Kondo S, Shimura Y, Tonozuka T, Sakano Y, Kamitori S |
| Title |
Role of Phe286 in the recognition mechanism of cyclomaltooligosaccharides (cyclodextrins) by Thermoactinomyces vulgaris R-47 alpha-amylase 2 (TVAII). |
| Related PDB | 1jf5 1jf6 |
| Related UniProtKB | |
| [15] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 11226882 |
| Journal | J Biochem (Tokyo) |
| Year | 2001 |
| Volume | 129 |
| Pages | 423-8 |
| Authors | Kondo S, Ohtaki A, Tonozuka T, Sakano Y, Kamitori S |
| Title |
Studies on the hydrolyzing mechanism for cyclodextrins of Thermoactinomyces vulgaris R-47 alpha-amylase 2 (TVAII). |
| Related PDB | 1g1y |
| Related UniProtKB | |
| [16] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11834127 |
| Journal | Biotechnol Appl Biochem |
| Year | 2002 |
| Volume | 35 |
| Pages | 27-34 |
| Authors | Cheong KA, Kim TJ, Yoon JW, Park CS, Lee TS, Kim YB, Park KH, Kim JW |
| Title |
Catalytic activities of intracellular dimeric neopullulanase on cyclodextrin, |
| Related PDB | |
| Related UniProtKB | |
| [17] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11923309 |
| Journal | J Biol Chem |
| Year | 2002 |
| Volume | 277 |
| Pages | 21891-7 |
| Authors | Lee HS, Kim MS, Cho HS, Kim JI, Kim TJ, Choi JH, Park C, Lee HS, Oh BH, Park KH |
| Title |
Cyclomaltodextrinase, |
| Related PDB | |
| Related UniProtKB | |
| [18] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS). |
| Medline ID | 22047855 |
| PubMed ID | 12051850 |
| Journal | J Mol Biol |
| Year | 2002 |
| Volume | 318 |
| Pages | 443-53 |
| Authors | Kamitori S, Abe A, Ohtaki A, Kaji A, Tonozuka T, Sakano Y |
| Title | Crystal structures and structural comparison of Thermoactinomyces vulgaris R-47 alpha-amylase 1 (TVAI) at 1.6 A resolution and alpha-amylase 2 (TVAII) at 2.3 A resolution. |
| Related PDB | 1ji2 |
| Related UniProtKB | Q08751 |
| [19] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12860426 |
| Journal | Carbohydr Res |
| Year | 2003 |
| Volume | 338 |
| Pages | 1553-8 |
| Authors | Ohtaki A, Iguchi A, Mizuno M, Tonozuka T, Sakano Y, Kamitori S |
| Title | Mutual conversion of substrate specificities of Thermoactinomyces vulgaris R-47 alpha-amylases TVAI and TVAII by site-directed mutagenesis. |
| Related PDB | |
| Related UniProtKB | |
| [20] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS). |
| Medline ID | |
| PubMed ID | 12547200 |
| Journal | J Mol Biol |
| Year | 2003 |
| Volume | 326 |
| Pages | 177-88 |
| Authors | Hondoh H, Kuriki T, Matsuura Y |
| Title | Three-dimensional structure and substrate binding of Bacillus stearothermophilus neopullulanase. |
| Related PDB | 1j0h 1j0i 1j0j 1j0k |
| Related UniProtKB | P38940 |
| [21] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 15182368 |
| Journal | Eur J Biochem |
| Year | 2004 |
| Volume | 271 |
| Pages | 2530-8 |
| Authors | Mizuno M, Tonozuka T, Uechi A, Ohtaki A, Ichikawa K, Kamitori S, Nishikawa A, Sakano Y |
| Title | The crystal structure of Thermoactinomyces vulgaris R-47 alpha-amylase II (TVA II) complexed with transglycosylated product. |
| Related PDB | 1vb9 |
| Related UniProtKB | |
| [22] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 15138257 |
| Journal | J Biol Chem |
| Year | 2004 |
| Volume | 279 |
| Pages | 31033-40 |
| Authors | Ohtaki A, Mizuno M, Tonozuka T, Sakano Y, Kamitori S |
| Title | Complex structures of Thermoactinomyces vulgaris R-47 alpha-amylase 2 with acarbose and cyclodextrins demonstrate the multiple substrate recognition mechanism. |
| Related PDB | 1vfk 1vfm 1vfo 1vfu |
| Related UniProtKB | |
| Comments |
|---|
|
This enzyme belongs to the glycosidase family-13.
Although this enzyme binds a calcium ion, According to the literature [10] & [22], Asp325 of 1bvz (PDB) acts as a nucleophile, (1) Glu354 initiates the reaction by protonating O4 atom, (2) Asp325 makes a nucleophilic attack on the C1 atom to form glycosyl-enzyme intermediate. (3) Glu354 acts as a general base to activate a water. (4) The activated water attacks on the intermediate to complete the hydrolysis. |
| Created | Updated |
|---|---|
| 2005-04-21 | 2009-02-26 |