DB code: S00205

RLCP classification	1.30.36020.973 : Hydrolysis
CATH domain	3.20.20.80 : TIM Barrel	Catalytic domain
E.C.	3.2.1.21
CSA	1cbg
M-CSA	1cbg
MACiE

CATH domain	Related DB codes (homologues)
3.20.20.80 : TIM Barrel	S00202 S00210 S00748 S00906 S00907 S00911 S00912 S00915 M00134 M00160 D00479 S00204 S00206 S00207 S00203 S00208 S00209 S00211 S00213 S00214 M00113 T00307 D00165 D00166 D00169 D00176 D00501 D00502 D00503 D00844 D00861 D00864 M00026 M00112 M00193 M00346 T00057 T00062 T00063 T00066 T00067

Uniprot Enzyme Name
UniprotKB	Protein name	Synonyms	CAZy	Pfam	RefSeq
P22073	Beta-glucosidase A	BGA EC 3.2.1.21 Gentiobiase Cellobiase Beta-D-glucoside glucohydrolase Amygdalase	GH1 (Glycoside Hydrolase Family 1)	PF00232 (Glyco_hydro_1) [Graphical View]
Q03506	Beta-glucosidase	EC 3.2.1.21 Gentiobiase Cellobiase Beta-D-glucoside glucohydrolase Amygdalase	GH1 (Glycoside Hydrolase Family 1)	PF00232 (Glyco_hydro_1) [Graphical View]
P26205	Cyanogenic beta-glucosidase	EC 3.2.1.21 Linamarase	GH1 (Glycoside Hydrolase Family 1)	PF00232 (Glyco_hydro_1) [Graphical View]
P49235	Beta-glucosidase, chloroplastic	EC 3.2.1.21 Gentiobiase Cellobiase Beta-D-glucoside glucohydrolase	GH1 (Glycoside Hydrolase Family 1)	PF00232 (Glyco_hydro_1) [Graphical View]	NP_001105454.1 (Protein) NM_001111984.1 (DNA/RNA sequence)

KEGG enzyme name
beta-glucosidase gentiobiase cellobiase emulsin elaterase aryl-beta-glucosidase beta-D-glucosidase beta-glucoside glucohydrolase arbutinase amygdalinase p-nitrophenyl beta-glucosidase primeverosidase amygdalase limarase salicilinase beta-1,6-glucosidase

UniprotKB: Accession Number	Entry name	Activity	Subunit	Subcellular location
P22073	BGLA_PAEPO	Hydrolysis of terminal, non-reducing beta-D- glucose residues with release of beta-D-glucose.	Homooctamer.
Q03506	BGLA_BACCI	Hydrolysis of terminal, non-reducing beta-D- glucosyl residues with release of beta-D-glucose.
P26205	BGLT_TRIRP	Hydrolysis of terminal, non-reducing beta-D- glucose residues with release of beta-D-glucose.	Homodimer.
P49235	BGLC_MAIZE	Hydrolysis of terminal, non-reducing beta-D- glucose residues with release of beta-D-glucose.	Homodimer.	Plastid, chloroplast.

KEGG Pathways
Map code	Pathways	E.C.
MAP00460	Cyanoamino acid metabolism
MAP00500	Starch and sucrose metabolism
MAP00940	Phenylpropanoid biosynthesis

Compound table
	Substrates		Products		Intermediates
KEGG-id	C00963	C00001	C00963	C00221
E.C.
Compound	beta-D-Glucoside	H2O	beta-D-Glucoside	beta-D-Glucose	Transition-state in deglycosylation
Type	carbohydrate	H2O	carbohydrate	carbohydrate
ChEBI		15377 15377		15903 15903
PubChem		22247451 962 22247451 962		64689 64689

1bgaA	Unbound		Unbound	Unbound
1bgaB	Unbound		Unbound	Unbound
1bgaC	Unbound		Unbound	Unbound
1bgaD	Unbound		Unbound	Unbound
1bggA	Unbound		Unbound	Unbound
1bggB	Unbound		Unbound	Analogue:GCO
1bggC	Unbound		Unbound	Analogue:GCO
1bggD	Unbound		Unbound	Analogue:GCO
1tr1A	Unbound		Unbound	Unbound
1tr1B	Unbound		Unbound	Unbound
1tr1C	Unbound		Unbound	Unbound
1tr1D	Unbound		Unbound	Unbound
1qoxA	Unbound		Unbound	Unbound
1qoxB	Unbound		Unbound	Unbound
1qoxC	Unbound		Unbound	Unbound
1qoxD	Unbound		Unbound	Unbound
1qoxE	Unbound		Unbound	Unbound
1qoxF	Unbound		Unbound	Unbound
1qoxG	Unbound		Unbound	Unbound
1qoxH	Unbound		Unbound	Unbound
1qoxI	Unbound		Unbound	Unbound
1qoxJ	Unbound		Unbound	Unbound
1qoxK	Unbound		Unbound	Unbound
1qoxL	Unbound		Unbound	Unbound
1qoxM	Unbound		Unbound	Unbound
1qoxN	Unbound		Unbound	Unbound
1qoxO	Unbound		Unbound	Unbound
1qoxP	Unbound		Unbound	Unbound
1cbgA	Unbound		Unbound	Unbound
1e1eA	Unbound		Unbound	Unbound
1e1eB	Unbound		Unbound	Unbound
1e1fA	Analogue:PSG		Unbound	Unbound
1e1fB	Analogue:PSG		Unbound	Unbound
1e4lA	Unbound		Unbound	Unbound
1e4lB	Unbound		Unbound	Unbound
1e4nA	Unbound		Unbound	Unbound
1e4nB	Unbound		Unbound	Unbound
1e55A	Unbound		Analogue:GLC-DHR	Unbound
1e55B	Unbound		Analogue:GLC-DHR	Unbound
1e56A	Unbound		Analogue:GLC-HBO	Unbound
1e56B	Unbound		Unbound	Unbound	Tansition-state-analogue:GLC-HBO

Reference for Active-site residues
resource	references	E.C.

Active-site residues
PDB	Catalytic residues	Cofactor-binding residues	Modified residues	Main-chain involved in catalysis	Comment

1bgaA	HIS 121;GLU 166;TYR 296;GLU 352
1bgaB	HIS 121;GLU 166;TYR 296;GLU 352
1bgaC	HIS 121;GLU 166;TYR 296;GLU 352
1bgaD	HIS 121;GLU 166;TYR 296;GLU 352
1bggA	HIS 121;GLU 166;TYR 296;GLU 352
1bggB	HIS 121;GLU 166;TYR 296;GLU 352
1bggC	HIS 121;GLU 166;TYR 296;GLU 352
1bggD	HIS 121;GLU 166;TYR 296;GLU 352
1tr1A	HIS 121;GLU 166;TYR 296;GLU 352
1tr1B	HIS 121;GLU 166;TYR 296;GLU 352
1tr1C	HIS 121;GLU 166;TYR 296;GLU 352
1tr1D	HIS 121;GLU 166;TYR 296;GLU 352
1qoxA	HIS 121;GLU 166;TYR 296;GLU 355
1qoxB	HIS 121;GLU 166;TYR 296;GLU 355
1qoxC	HIS 121;GLU 166;TYR 296;GLU 355
1qoxD	HIS 121;GLU 166;TYR 296;GLU 355
1qoxE	HIS 121;GLU 166;TYR 296;GLU 355
1qoxF	HIS 121;GLU 166;TYR 296;GLU 355
1qoxG	HIS 121;GLU 166;TYR 296;GLU 355
1qoxH	HIS 121;GLU 166;TYR 296;GLU 355
1qoxI	HIS 121;GLU 166;TYR 296;GLU 355
1qoxJ	HIS 121;GLU 166;TYR 296;GLU 355
1qoxK	HIS 121;GLU 166;TYR 296;GLU 355
1qoxL	HIS 121;GLU 166;TYR 296;GLU 355
1qoxM	HIS 121;GLU 166;TYR 296;GLU 355
1qoxN	HIS 121;GLU 166;TYR 296;GLU 355
1qoxO	HIS 121;GLU 166;TYR 296;GLU 355
1qoxP	HIS 121;GLU 166;TYR 296;GLU 355
1cbgA	HIS 137;GLU 183;TYR 326;GLU 397
1e1eA	HIS 142;GLU 191;TYR 333;GLU 406
1e1eB	HIS 142;GLU 191;TYR 333;GLU 406
1e1fA	HIS 142;GLU 191;TYR 333;GLU 406
1e1fB	HIS 142;GLU 191;TYR 333;GLU 406
1e4lA	HIS 142;;TYR 333;GLU 406				mutant E191D
1e4lB	HIS 142;;TYR 333;GLU 406				mutant E191D
1e4nA	HIS 142;;TYR 333;GLU 406				mutant E191D
1e4nB	HIS 142;;TYR 333;GLU 406				mutant E191D
1e55A	HIS 142;;TYR 333;GLU 406				mutant E191D
1e55B	HIS 142;;TYR 333;GLU 406				mutant E191D
1e56A	HIS 142;;TYR 333;GLU 406				mutant E191D
1e56B	HIS 142;;TYR 333;GLU 406				mutant E191D

References for Catalytic Mechanism
References	Sections	No. of steps in catalysis
[9]
[10]
[14]	Scheme 1
[15]	p.17175-17176
[16]	Fig.8
[20]	p.495-500
[33]
[34]

References
[1]
Resource
Comments
Medline ID
PubMed ID	1367638
Journal	Enzyme Microb Technol
Year	1991
Volume	13
Pages	547-53
Authors	Cantarella M, Cantarella L, Alfani F
Title	Hydrolytic reactions in two-phase systems. Effect of water-immiscible organic solvents on stability and activity of acid phosphatase, beta-glucosidase, and beta-fructofuranosidase.
Related PDB
Related UniProtKB
[2]
Resource
Comments
Medline ID
PubMed ID	1714449
Journal	J Biol Chem
Year	1991
Volume	266
Pages	15021-7
Authors	Fabbro D, Grabowski GA
Title	Human acid beta-glucosidase. Use of inhibitory and activating monoclonal antibodies to investigate the enzyme's catalytic mechanism and saposin A and C binding sites.
Related PDB
Related UniProtKB
[3]
Resource
Comments
Medline ID
PubMed ID	8477701
Journal	Eur J Biochem
Year	1993
Volume	213
Pages	305-12
Authors	Kengen SW, Luesink EJ, Stams AJ, Zehnder AJ
Title	Purification and characterization of an extremely thermostable beta-glucosidase from the hyperthermophilic archaeon Pyrococcus furiosus.
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID	7986074
Journal	Arch Biochem Biophys
Year	1994
Volume	315
Pages	323-30
Authors	Keresztessy Z, Kiss L, Hughes MA
Title	Investigation of the active site of the cyanogenic beta-D-glucosidase (linamarase) from Manihot esculenta Crantz (cassava). II. Identification of Glu-198 as an active site carboxylate group with acid catalytic function.
Related PDB
Related UniProtKB
[5]
Resource
Comments
Medline ID
PubMed ID	7944386
Journal	Arch Biochem Biophys
Year	1994
Volume	314
Pages	142-52
Authors	Keresztessy Z, Kiss L, Hughes MA
Title	Investigation of the active site of the cyanogenic beta-D-glucosidase (linamarase) from Manihot esculenta Crantz (cassava). I. Evidence for an essential carboxylate and a reactive histidine residue in a single catalytic center.
Related PDB
Related UniProtKB
[6]
Resource
Comments
Medline ID
PubMed ID	8028010
Journal	J Mol Biol
Year	1994
Volume	240
Pages	267-70
Authors	Sanz-Aparicio J, Romero A, Martinez-Ripoll M, Madarro A, Flors A, Polaina J
Title	Crystallization and preliminary X-ray diffraction analysis of a type I beta-glucosidase encoded by the bgIA gene of Bacillus polymyxa.
Related PDB
Related UniProtKB
[7]
Resource
Comments
Medline ID
PubMed ID	8588743
Journal	Arch Microbiol
Year	1995
Volume	164
Pages	414-9
Authors	Rath J, Messner R, Kosma P, Altmann F, Marz L, Kubicek CP
Title	The alpha-D-mannan core of a complex cell-wall heteroglycan of Trichoderma reesei is responsible for beta-glucosidase activation.
Related PDB
Related UniProtKB
[8]
Resource
Comments
Medline ID
PubMed ID	8519777
Journal	Biochemistry
Year	1995
Volume	34
Pages	16194-202
Authors	Namchuk MN, Withers SG
Title	Mechanism of Agrobacterium beta-glucosidase: kinetic analysis of the role of noncovalent enzyme/substrate interactions.
Related PDB
Related UniProtKB
[9]
Resource
Comments
Medline ID
PubMed ID	7578061
Journal	Biochemistry
Year	1995
Volume	34
Pages	14554-62
Authors	Wang Q, Trimbur D, Graham R, Warren RA, Withers SG
Title	Identification of the acid/base catalyst in Agrobacterium faecalis beta-glucosidase by kinetic analysis of mutants.
Related PDB
Related UniProtKB
[10]
Resource
Comments	X-ray crystallography (2.15 angstroms).
Medline ID	96097401
PubMed ID	8535788
Journal	Structure
Year	1995
Volume	3
Pages	951-60
Authors	Barrett T, Suresh CG, Tolley SP, Dodson EJ, Hughes MA
Title	The crystal structure of a cyanogenic beta-glucosidase from white clover, a family 1 glycosyl hydrolase.
Related PDB	1cbg
Related UniProtKB	P26205
[11]
Resource
Comments
Medline ID
PubMed ID	9636318
Journal	Biotechnology (N Y)
Year	1996
Volume	14
Pages	88-91
Authors	Fischer L, Bromann R, Kengen SW, de Vos WM, Wagner F
Title	Catalytical potency of beta-glucosidase from the extremophile Pyrococcus furiosus in glucoconjugate synthesis.
Related PDB
Related UniProtKB
[12]
Resource
Comments
Medline ID
PubMed ID	9214768
Journal	Biosci Biotechnol Biochem
Year	1997
Volume	61
Pages	1033-5
Authors	Nunoura N, Fujita T, Ohdan K, Kirihata M, Yamamoto K, Kumagai H
Title	Structural analysis of disaccharides synthesized by beta-D-glucosidase of Bifidobacterium breve clb and their assimilation by Bifidobacteria.
Related PDB
Related UniProtKB
[13]
Resource
Comments
Medline ID
PubMed ID	9657966
Journal	Biochem J
Year	1998
Volume	333
Pages	275-83
Authors	Duroux L, Delmotte FM, Lancelin JM, Keravis G, Jay-Allemand C
Title	Insight into naphthoquinone metabolism: beta-glucosidase-catalysed hydrolysis of hydrojuglone beta-D-glucopyranoside.
Related PDB
Related UniProtKB
[14]
Resource
Comments
Medline ID
PubMed ID	9461511
Journal	Biochem J
Year	1998
Volume	330
Pages	203-9
Authors	Lawson SL, Warren RA, Withers SG
Title	Mechanistic consequences of replacing the active-site nucleophile Glu-358 in Agrobacterium sp. beta-glucosidase with a cysteine residue.
Related PDB
Related UniProtKB
[15]
Resource
Comments
Medline ID
PubMed ID	9860830
Journal	Biochemistry
Year	1998
Volume	37
Pages	17170-8
Authors	Bauer MW, Kelly RM
Title	The family 1 beta-glucosidases from Pyrococcus furiosus and Agrobacterium faecalis share a common catalytic mechanism.
Related PDB
Related UniProtKB
[16]
Resource
Comments
Medline ID
PubMed ID	9630531
Journal	Biochim Biophys Acta
Year	1998
Volume	1385
Pages	78-88
Authors	Watt DK, Ono H, Hayashi K
Title	Agrobacterium tumefaciens beta-glucosidase is also an effective beta-xylosidase, and has a high transglycosylation activity in the presence of alcohols.
Related PDB
Related UniProtKB
[17]
Resource
Comments
Medline ID
PubMed ID	9845479
Journal	Biol Trace Elem Res
Year	1998
Volume	64
Pages	247-58
Authors	Sreekala M, Lalitha K
Title	Selenium-mediated differential response of beta-glucosidase and beta-galactosidase of germinating Trigonella foenum-graecum.
Related PDB
Related UniProtKB
[18]
Resource
Comments
Medline ID
PubMed ID	9468315
Journal	FEBS Lett
Year	1998
Volume	421
Pages	243-8
Authors	Montero E, Vallmitjana M, Perez-Pons JA, Querol E, Jimenez-Barbero J, Canada FJ
Title	NMR studies of the conformation of thiocellobiose bound to a beta-glucosidase from Streptomyces sp.
Related PDB
Related UniProtKB
[19]
Resource
Comments
Medline ID
PubMed ID	9857024
Journal	J Biol Chem
Year	1998
Volume	273
Pages	34941-8
Authors	Hays WS, VanderJagt DJ, Bose B, Serianni AS, Glew RH
Title	Catalytic mechanism and specificity for hydrolysis and transglycosylation reactions of cytosolic beta-glucosidase from guinea pig liver.
Related PDB
Related UniProtKB
[20]
Resource
Comments	X-ray crystallography (2.4 angstroms).
Medline ID	98139963
PubMed ID	9466926
Journal	J Mol Biol
Year	1998
Volume	275
Pages	491-502
Authors	Sanz-Aparicio J, Hermoso JA, Martinez-Ripoll M, Lequerica JL, Polaina J
Title	Crystal structure of beta-glucosidase A from Bacillus polymyxa: insights into the catalytic activity in family 1 glycosyl hydrolases.
Related PDB	1bga 1bgg 1tr1
Related UniProtKB	P22073
[21]
Resource
Comments
Medline ID
PubMed ID	9849940
Journal	Proteins
Year	1998
Volume	33
Pages	567-76
Authors	Sanz-Aparicio J, Hermoso JA, Martinez-Ripoll M, Gonzalez B, Lopez-Camacho C, Polaina J
Title	Structural basis of increased resistance to thermal denaturation induced by single amino acid substitution in the sequence of beta-glucosidase A from Bacillus polymyxa.
Related PDB
Related UniProtKB
[22]
Resource
Comments
Medline ID
PubMed ID	10467123
Journal	Biotechnol Appl Biochem
Year	1999
Volume	30
Pages	81-7
Authors	Lin J, Pillay B, Singh S
Title	Purification and biochemical characteristics of beta-D-glucosidase from a thermophilic fungus, Thermomyces lanuginosus-SSBP.
Related PDB
Related UniProtKB
[23]
Resource
Comments
Medline ID
PubMed ID	10583402
Journal	Eur J Biochem
Year	1999
Volume	266
Pages	1056-65
Authors	Rotrekl V, Nejedla E, Kucera I, Abdallah F, Palme K, Brzobohaty B
Title	The role of cysteine residues in structure and enzyme activity of a maize beta-glucosidase.
Related PDB
Related UniProtKB
[24]
Resource
Comments
Medline ID
PubMed ID	10737327
Journal	Glycoconj J
Year	1999
Volume	16
Pages	415-23
Authors	Kono H, Kawano S, Tajima K, Erata T, Takai M
Title	Structural analyses of new tri- and tetrasaccharides produced from disaccharides by transglycosylation of purified Trichoderma viride beta-glucosidase.
Related PDB
Related UniProtKB
[25]
Resource
Comments
Medline ID
PubMed ID	10736164
Journal	Biochemistry
Year	2000
Volume	39
Pages	3656-65
Authors	Lebbink JH, Kaper T, Bron P, van der Oost J, de Vos WM
Title	Improving low-temperature catalysis in the hyperthermostable Pyrococcus furiosus beta-glucosidase CelB by directed evolution.
Related PDB
Related UniProtKB
[26]
Resource
Comments
Medline ID
PubMed ID	11828410
Journal	Chembiochem
Year	2000
Volume	1
Pages	177-80
Authors	Hansen SU, Plesner IW, Bols M
Title	Direct NMR-spectroscopic determination of active-enzyme concentration by titration with a labeled inhibitor: determination of the k(cat) value of almond beta-glucosidase.
Related PDB
Related UniProtKB
[27]
Resource
Comments
Medline ID
PubMed ID	11052758
Journal	J Agric Food Chem
Year	2000
Volume	48
Pages	4929-36
Authors	Decker CH, Visser J, Schreier P
Title	beta-glucosidases from five black Aspergillus species: study of their physico-chemical and biocatalytic properties.
Related PDB
Related UniProtKB
[28]
Resource
Comments
Medline ID
PubMed ID	10871610
Journal	J Biol Chem
Year	2000
Volume	275
Pages	28843-8
Authors	Arrizubieta MJ, Polaina J
Title	Increased thermal resistance and modification of the catalytic properties of a beta-glucosidase by random mutagenesis and in vitro recombination.
Related PDB
Related UniProtKB
[29]
Resource
Comments	X-ray crystallography (2.7 Angstroms)
Medline ID
PubMed ID	10675298
Journal	J Struct Biol
Year	2000
Volume	129
Pages	69-79
Authors	Hakulinen N, Paavilainen S, Korpela T, Rouvinen J
Title	The Crystal Structure of Beta-Glucosidase from Bacillus Circulans Sp. Alkalophilus: Ability to Form Long Polymeric Assemblies.
Related PDB	1qox
Related UniProtKB
[30]
Resource
Comments	X-ray crystallography (2.0-2.2 Angstroms)
Medline ID
PubMed ID	11106394
Journal	Proc Nat Acad Sci USA
Year	2000
Volume	97
Pages	13555-13560
Authors	Czjzek M, Cicek M, Zamboni V, Bevan DR, Henrissat B, Esen A
Title	The Mechanism of Substrate (Aglycone) Specificity in Beta-Glucosidases is Revealed by Crystal Structures of Mutant Maize Beta-Glucosidase-Dimboa,-Dimboaglc, and-Dhurrin Complexes.
Related PDB	1e4l 1e4n 1e55 1e56
Related UniProtKB
[31]
Resource
Comments	X-ray crystallography (2.5 Angstroms)
Medline ID
PubMed ID	11171077
Journal	Biochem J
Year	2001
Volume	354
Pages	37-46
Authors	Czjzek M, Cicek M, Zamboni V, Burmeister WP, Bevan DR, Henrissat B, Esen A
Title	Crystal Structure of a Monocotyledon (Maize Zmglu1) Beta-Glucosidase and a Model of its Complex with P-Nitrophenyl Beta-D-Thioglucoside.
Related PDB	1e1e 1e1f
Related UniProtKB
[32]
Resource
Comments
Medline ID
PubMed ID	11115405
Journal	Biochem J
Year	2001
Volume	353
Pages	117-127
Authors	Parry NJ, Beever DE, Owen E, Vandenberghe I, Van Beeumen J, Bhat MK
Title	Biochemical characterization and mechanism of action of a thermostable beta-glucosidase purified from Thermoascus aurantiacus.
Related PDB
Related UniProtKB
[33]
Resource
Comments	catalysis, kinetics
Medline ID
PubMed ID	11352732
Journal	Biochemistry
Year	2001
Volume	40
Pages	5975-82
Authors	Vallmitjana M, Ferrer-Navarro M, Planell R, Abel M, Ausin C, Querol E, Planas A, Perez-Pons JA
Title	Mechanism of the family 1 beta-glucosidase from Streptomyces sp: catalytic residues and kinetic studies.
Related PDB
Related UniProtKB
[34]
Resource
Comments
Medline ID
PubMed ID	11342030
Journal	Biochim Biophys Acta
Year	2001
Volume	1545
Pages	41-52
Authors	Marana SR, Jacobs-Lorena M, Terra WR, Ferreira C
Title	Amino acid residues involved in substrate binding and catalysis in an insect digestive beta-glycosidase.
Related PDB
Related UniProtKB
[35]
Resource
Comments
Medline ID
PubMed ID	11520685
Journal	Insect Biochem Mol Biol
Year	2001
Volume	31
Pages	1065-76
Authors	Ferreira AH, Marana SR, Terra WR, Ferreira C
Title	Purification, molecular cloning, and properties of a beta-glycosidase isolated from midgut lumen of Tenebrio molitor (Coleoptera) larvae.
Related PDB
Related UniProtKB
[36]
Resource
Comments
Medline ID
PubMed ID	11706179
Journal	Plant Physiol
Year	2001
Volume	127
Pages	973-85
Authors	Zouhar J, Vevodova J, Marek J, Damborsky J, Su XD, Brzobohaty B
Title	Insights into the functional architecture of the catalytic center of a maize beta-glucosidase Zm-p60.1.
Related PDB
Related UniProtKB
[37]
Resource
Comments
Medline ID
PubMed ID	12450115
Journal	Biosci Biotechnol Biochem
Year	2002
Volume	66
Pages	2060-7
Authors	Fukuda K, Mori H, Okuyama M, Kimura A, Ozaki H, Yoneyama M, Chiba S
Title	Identification of essential ionizable groups and evaluation of subsite affinities in the active site of beta-D-glucosidase F1 from a Streptomyces sp.
Related PDB
Related UniProtKB
[38]
Resource
Comments
Medline ID
PubMed ID	12363364
Journal	Biotechnol Prog
Year	2002
Volume	18
Pages	1104-8
Authors	Maugard T, Enaud E, de la Sayette A, Choisy P, Legoy MD
Title	Beta-glucosidase-catalyzed hydrolysis of indican from leaves of Polygonum tinctorium.
Related PDB
Related UniProtKB

Comments
This enzyme belongs to the family-1 of glycosidase enzymes, a member family of 4/7 superfamily, which has got the catalytic residues at the C-terminal ends of beta-4 and beta-7 on the (alpha/beta)8 barrel fold. According to the literature [10] & [20], Glu352 and Glu166 (of PDB;1bgaA) act as a nucleophile and an acid-base, respectively. Moreover, the literature suggested that His121 might stabilize the transition-state, and that Tyr296 modulates the pKa and the activity of the nucleophile, Glu352.

Comments

This enzyme belongs to the family-1 of glycosidase enzymes, a member family of 4/7 superfamily, which has got the catalytic residues at the C-terminal ends of beta-4 and beta-7 on the (alpha/beta)8 barrel fold.
According to the literature [10] & [20], Glu352 and Glu166 (of PDB;1bgaA) act as a nucleophile and an acid-base, respectively. Moreover, the literature suggested that His121 might stabilize the transition-state, and that Tyr296 modulates the pKa and the activity of the nucleophile, Glu352.

Created	Updated
2003-12-05	2009-03-17