DB code: M00344

RLCP classification 3.103.130000.1162 : Transfer
CATH domain 2.30.29.30 : PH-domain like
-.-.-.- :
-.-.-.- :
3.30.505.10 : SHC Adaptor Protein
3.30.200.20 : Phosphorylase Kinase; domain 1
1.10.510.10 : Transferase(Phosphotransferase); domain 1 Catalytic domain
E.C. 2.7.10.2
CSA
M-CSA
MACiE

CATH domain Related DB codes (homologues)
1.10.510.10 : Transferase(Phosphotransferase); domain 1 M00125 M00124 M00131 T00224 M00127 M00129 M00130 M00132 M00136 M00196 M00197 M00198 M00304 M00323 M00325 M00326 M00327 M00328 M00329 M00330 M00331 M00332 M00333 M00335 M00339
2.30.29.30 : PH-domain like M00125 M00183 M00118
3.30.200.20 : Phosphorylase Kinase; domain 1 M00125 M00124 M00131 T00224 M00127 M00129 M00130 M00132 M00136 M00196 M00197 M00198 M00304 M00323 M00325 M00326 M00327 M00328 M00329 M00330 M00331 M00332 M00333 M00335 M00339 D00298
3.30.505.10 : SHC Adaptor Protein M00183 M00043 M00130 M00148 T00256 M00304 M00333 M00339 T00221

Uniprot Enzyme Name
UniprotKB Protein name Synonyms RefSeq Pfam
P51813 Cytoplasmic tyrosine-protein kinase BMX
EC 2.7.10.2
Bone marrow tyrosine kinase gene in chromosome X protein
Epithelial and endothelial tyrosine kinase
ETK
NTK38
NP_001712.1 (Protein)
NM_001721.6 (DNA/RNA sequence)
NP_975010.1 (Protein)
NM_203281.2 (DNA/RNA sequence)
PF00779 (BTK)
PF00169 (PH)
PF07714 (Pkinase_Tyr)
PF00017 (SH2)
[Graphical View]

KEGG enzyme name
Non-specific protein-tyrosine kinase
ATP:protein-tyrosine O-phosphotransferase (ambiguous)
Bruton's tyrosine kinase
BMX
Cytoplasmic protein tyrosine kinase
ETK
Ntk

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P51813 BMX_HUMAN ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. Interacts with BCAR1, CAV1, MYD88, PTK2/FAK1, RUFY1, RUFY2, STAT3, TIRAP and TNFRSF1B. Cytoplasm. Note: Localizes to the edges of spreading cells when complexed with BCAR1. Binds 1 zinc ion per subunit (By similarity).

KEGG Pathways
Map code Pathways E.C.

Compound table
Cofactors Substrates Products Intermediates
KEGG-id C00305 C00002 C00585 C00008 C01167
E.C.
Compound Magnesium ATP [protein]-L-tyrosine ADP [protein]-L-tyrosine phosphate
Type divalent metal (Ca2+, Mg2+) amine group,nucleotide aromatic ring (only carbon atom),peptide/protein amine group,nucleotide aromatic ring (only carbon atom),peptide/protein,phosphate group/phosphate ion
ChEBI 18420
 18420
 		15422
 15422
 		16761
 16761
PubChem 888
 888
 		5957
 5957
 		6022
 6022
2ys2A Unbound Unbound Unbound Unbound Unbound
2ekxA00 Unbound Unbound Unbound Unbound Unbound
3sxrA01 Unbound Unbound Unbound Unbound Unbound
3sxrB01 Unbound Unbound Unbound Unbound Unbound
3sxsA01 Unbound Unbound Unbound Unbound Unbound
3sxrA02 Unbound Unbound Unbound Unbound Unbound
3sxrB02 Unbound Unbound Unbound Unbound Unbound
3sxsA02 Unbound Unbound Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
2ys2A
2ekxA00
3sxrA01
3sxrB01
3sxsA01
3sxrA02 ASP 536;ARG 540 ASN 541;ASP 554(Magnesium binding)
3sxrB02 ASP 536;ARG 540 ASN 541;ASP 554(Magnesium binding)
3sxsA02 ASP 536;ARG 540 ASN 541;ASP 554(Magnesium binding)

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[1]
Figure 2

References
[1]
Resource
Comments REVIEW ON FUNCTION.
Medline ID
PubMed ID 11114746
Journal Oncogene
Year 2000
Volume 19
Pages 5651-61
Authors Qiu Y, Kung HJ
Title Signaling network of the Btk family kinases.
Related PDB
Related UniProtKB
[2]
Resource
Comments
Medline ID
PubMed ID 11331870
Journal Nat Cell Biol
Year 2001
Volume 3
Pages 439-44
Authors Chen R, Kim O, Li M, Xiong X, Guan JL, Kung HJ, Chen H, Shimizu Y, Qiu Y
Title Regulation of the PH-domain-containing tyrosine kinase Etk by focal adhesion kinase through the FERM domain.
Related PDB
Related UniProtKB
[3]
Resource
Comments
Medline ID
PubMed ID 14532277
Journal J Biol Chem
Year 2003
Volume 278
Pages 51267-76
Authors Zhang R, Xu Y, Ekman N, Wu Z, Wu J, Alitalo K, Min W
Title Etk/Bmx transactivates vascular endothelial growth factor 2 and recruits phosphatidylinositol 3-kinase to mediate the tumor necrosis factor-induced angiogenic pathway.
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID 18292575
Journal J Immunol
Year 2008
Volume 180
Pages 3485-91
Authors Semaan N, Alsaleh G, Gottenberg JE, Wachsmann D, Sibilia J
Title Etk/BMX, a Btk family tyrosine kinase, and Mal contribute to the cross-talk between MyD88 and FAK pathways.
Related PDB
Related UniProtKB
[5]
Resource
Comments
Medline ID
PubMed ID 21883956
Journal Chem Biol Drug Des
Year 2011
Volume 78
Pages 739-48
Authors Muckelbauer J, Sack JS, Ahmed N, Burke J, Chang CY, Gao M, Tino J, Xie D, Tebben AJ
Title X-ray crystal structure of bone marrow kinase in the x chromosome: a Tec family kinase.
Related PDB 3sxr 3sxs
Related UniProtKB P51813

Comments
This enzyme belongs to Tec family of non-receptor tyrosine kinases.
This enzyme is composed of the N-terminal pleckstrin homology (PH) domain, BTK-type zinc finger motif, an unknown domain, which can be Src homology 3 (SH3) domain, Src homology 2 (SH2) domain, and the C-terminal kinase domain (see [1]).
The catalytic domain of this enzyme is homologous to Proto-oncogene tyrosine-protein kinase ABL1 (M00130 in EzCatDB), with common active-site residues.

Created Updated
2012-06-19 2013-01-30