DB code: D00298
| CATH domain | 3.30.200.20 : Phosphorylase Kinase; domain 1 | |
|---|---|---|
| 3.30.470.20 : D-amino Acid Aminotransferase; Chain A, domain 1 | ||
| E.C. | 6.3.2.6 | |
| CSA | ||
| M-CSA | ||
| MACiE | ||
| CATH domain | Related DB codes (homologues) |
|---|---|
| 3.30.200.20 : Phosphorylase Kinase; domain 1 | M00125 M00124 M00131 T00224 M00127 M00129 M00130 M00132 M00136 M00196 M00197 M00198 M00304 M00323 M00325 M00326 M00327 M00328 M00329 M00330 M00331 M00332 M00333 M00335 M00339 M00344 |
| 3.30.470.20 : D-amino Acid Aminotransferase; Chain A, domain 1 | T00082 M00035 M00037 M00051 T00107 T00108 |
| Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | RefSeq | Pfam |
|---|---|---|---|---|
| P27616 |
Phosphoribosylaminoimidazole-succinocarboxamide synthase
|
EC
6.3.2.6
SAICAR synthetase |
NP_009409.1
(Protein)
NM_001178216.1 (DNA/RNA sequence) |
PF01259
(SAICAR_synt)
[Graphical View] |
| KEGG enzyme name |
|---|
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phosphoribosylaminoimidazolesuccinocarboxamide synthase
phosphoribosylaminoimidazole-succinocarboxamide synthetase PurC SAICAR synthetase 4-(N-succinocarboxamide)-5-aminoimidazole synthetase 4-[(N-succinylamino)carbonyl]-5-aminoimidazole ribonucleotidesynthetase SAICARs phosphoribosylaminoimidazolesuccinocarboxamide synthetase 5-aminoimidazole-4-N-succinocarboxamide ribonucleotide synthetase |
| UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
|---|---|---|---|---|---|
| P27616 | PUR7_YEAST | ATP + 5-amino-1-(5-phospho-D- ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4- carboxamido)succinate. | Monomer. |
| KEGG Pathways | Map code | Pathways | E.C. |
|---|---|---|
| MAP00230 | Purine metabolism |
| Compound table | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cofactors | Substrates | Products | Intermediates | ||||||||||
| KEGG-id | C00305 | C00002 | C04751 | C00049 | C00008 | C00009 | C04823 | ||||||
| E.C. | |||||||||||||
| Compound | Magnesium | ATP | 1-(5'-Phosphoribosyl)-5-amino-4-carboxyimidazole | L-Aspartate | ADP | Orthophosphate | 1-(5'-Phosphoribosyl)-5-amino-4-(N-succinocarboxamide)-imidazole | ||||||
| Type | divalent metal (Ca2+, Mg2+) | amine group,nucleotide | amine group,carboxyl group,nucleotide | amino acids,carboxyl group | amine group,nucleotide | phosphate group/phosphate ion | amino acids,amide group,amine group,carbohydrate,nucleotide | ||||||
| ChEBI |
18420 18420 |
15422 15422 |
28413 28413 |
17053 17053 |
16761 16761 |
26078 26078 |
18319 18319 |
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| PubChem |
888 888 |
5957 5957 |
165388 165388 |
44367445 5960 44367445 5960 |
6022 6022 |
1004 22486802 1004 22486802 |
160666 160666 |
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| 1a48A01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1a48A02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| Reference for Active-site residues | ||
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| resource | references | E.C. |
| Swiss-prot;P27616 | ||
| Active-site residues | ||||||||||
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| PDB | Catalytic residues | Cofactor-binding residues | Modified residues | Main-chain involved in catalysis | Comment | |||||
| 1a48A01 |
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| 1a48A02 |
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| References for Catalytic Mechanism | ||
|---|---|---|
| References | Sections | No. of steps in catalysis |
| References | |
|---|---|
| [1] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1534690 |
| Journal | Biochemistry |
| Year | 1992 |
| Volume | 31 |
| Pages | 5022-32 |
| Authors | Meyer E, Leonard NJ, Bhat B, Stubbe J, Smith JM |
| Title |
Purification and characterization of the purE, |
| Related PDB | |
| Related UniProtKB | |
| [2] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1447788 |
| Journal | J Mol Biol |
| Year | 1992 |
| Volume | 228 |
| Pages | 298-9 |
| Authors | Grebenko AI, Levdikov VM, Barynin VV, Melik-Adamyan WR, Myasnikov AN |
| Title | Crystallization and preliminary X-ray investigation of phosphoribosylaminoimidazolesuccinocarboxamide synthase from the yeast Saccharomyces cerevisiae. |
| Related PDB | |
| Related UniProtKB | |
| [3] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7918411 |
| Journal | Biochemistry |
| Year | 1994 |
| Volume | 33 |
| Pages | 11927-34 |
| Authors | Firestine SM, Poon SW, Mueller EJ, Stubbe J, Davisson VJ |
| Title | Reactions catalyzed by 5-aminoimidazole ribonucleotide carboxylases from Escherichia coli and Gallus gallus: a case for divergent catalytic mechanisms. |
| Related PDB | |
| Related UniProtKB | |
| [4] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) |
| Medline ID | 98212921 |
| PubMed ID | 9551557 |
| Journal | Structure |
| Year | 1998 |
| Volume | 6 |
| Pages | 363-76 |
| Authors | Levdikov VM, Barynin VV, Grebenko AI, Melik-Adamyan WR, Lamzin VS, Wilson KS |
| Title | The structure of SAICAR synthase: an enzyme in the de novo pathway of purine nucleotide biosynthesis. |
| Related PDB | 1a48 |
| Related UniProtKB | P27616 |
| Comments |
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| Created | Updated |
|---|---|
| 2004-03-25 | 2009-02-26 |