DB code: M00043
| RLCP classification | 3.103.106000.1163 : Transfer | |
|---|---|---|
| CATH domain | 3.10.20.90 : Ubiquitin-like (UB roll) | |
| 2.60.40.150 : Immunoglobulin-like | ||
| 1.25.40.70 : Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat | ||
| 3.30.1010.10 : Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 4 | Catalytic domain | |
| 1.10.1070.11 : Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, Domain 5 | Catalytic domain | |
| 2.30.30.40 : SH3 type barrels. | ||
| 1.10.555.10 : Phosphatidylinositol 3-kinase; Chain A | ||
| 3.30.505.10 : SHC Adaptor Protein | ||
| 3.30.505.10 : SHC Adaptor Protein | ||
| E.C. | 2.7.1.153 | |
| CSA | ||
| M-CSA | ||
| MACiE | ||
| CATH domain | Related DB codes (homologues) |
|---|---|
| 2.30.30.40 : SH3 type barrels. | M00183 M00130 T00256 M00304 M00335 |
| 2.60.40.150 : Immunoglobulin-like | M00221 M00183 M00118 |
| 3.30.505.10 : SHC Adaptor Protein | M00183 M00130 M00148 T00256 M00304 M00333 M00339 M00344 T00221 |
| Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | RefSeq | Pfam |
|---|---|---|---|---|
| P32871 |
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
|
PI3-kinase subunit alpha
PI3K-alpha PI3Kalpha PtdIns-3-kinase subunit alpha EC 2.7.1.153 Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha PtdIns-3-kinase subunit p110-alpha p110alpha Phosphoinositide-3-kinase catalytic alpha polypeptide Serine/threonine protein kinase PIK3CA EC 2.7.11.1 |
NP_776999.1
(Protein)
NM_174574.1 (DNA/RNA sequence) |
PF00454
(PI3_PI4_kinase)
PF00792 (PI3K_C2) PF02192 (PI3K_p85B) PF00794 (PI3K_rbd) PF00613 (PI3Ka) [Graphical View] |
| O02697 |
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform
|
PI3-kinase subunit gamma
PI3K-gamma PI3Kgamma PtdIns-3-kinase subunit gamma EC 2.7.1.153 Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma PtdIns-3-kinase subunit p110-gamma p110gamma Phosphoinositide-3-kinase catalytic gamma polypeptide Serine/threonine protein kinase PIK3CG EC 2.7.11.1 p120-PI3K |
NP_999104.1
(Protein)
NM_213939.1 (DNA/RNA sequence) |
PF00454
(PI3_PI4_kinase)
PF00792 (PI3K_C2) PF00794 (PI3K_rbd) PF00613 (PI3Ka) [Graphical View] |
| P48736 |
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform
|
PI3-kinase subunit gamma
PI3K-gamma PI3Kgamma PtdIns-3-kinase subunit gamma EC 2.7.1.153 Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma PtdIns-3-kinase subunit p110-gamma p110gamma Phosphoinositide-3-kinase catalytic gamma polypeptide Serine/threonine protein kinase PIK3CG EC 2.7.11.1 p120-PI3K |
NP_002640.2
(Protein)
NM_002649.2 (DNA/RNA sequence) |
PF00454
(PI3_PI4_kinase)
PF00792 (PI3K_C2) PF00794 (PI3K_rbd) PF00613 (PI3Ka) [Graphical View] |
| P23727 |
Phosphatidylinositol 3-kinase regulatory subunit alpha
|
PI3-kinase regulatory subunit alpha
PI3K regulatory subunit alpha PtdIns-3-kinase regulatory subunit alpha Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha PI3-kinase subunit p85-alpha PtdIns-3-kinase regulatory subunit p85-alpha |
NP_777000.1
(Protein)
NM_174575.1 (DNA/RNA sequence) |
PF00620
(RhoGAP)
PF00017 (SH2) PF07653 (SH3_2) [Graphical View] |
| P27986 |
Phosphatidylinositol 3-kinase regulatory subunit alpha
|
PI3-kinase regulatory subunit alpha
PI3K regulatory subunit alpha PtdIns-3-kinase regulatory subunit alpha Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha PI3-kinase subunit p85-alpha PtdIns-3-kinase regulatory subunit p85-alpha |
NP_001229395.1
(Protein)
NM_001242466.1 (DNA/RNA sequence) NP_852556.2 (Protein) NM_181504.3 (DNA/RNA sequence) NP_852664.1 (Protein) NM_181523.2 (DNA/RNA sequence) NP_852665.1 (Protein) NM_181524.1 (DNA/RNA sequence) |
PF00620
(RhoGAP)
PF00017 (SH2) PF07653 (SH3_2) [Graphical View] |
| KEGG enzyme name |
|---|
|
phosphatidylinositol-4,5-bisphosphate 3-kinase
type I phosphoinositide 3-kinase |
| UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
|---|---|---|---|---|---|
| P32871 | PK3CA_BOVIN | ATP + 1-phosphatidyl-1D-myo-inositol 4,5- bisphosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,4,5- trisphosphate. | Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit. Binds to IRS1 in nuclear extracts. Interacts with RUFY3 (By similarity). | ||
| O02697 | PK3CG_PIG | ATP + 1-phosphatidyl-1D-myo-inositol 4,5- bisphosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,4,5- trisphosphate. | Heterodimer of a catalytic subunit (PIK3CG/p120) and a regulatory (PIK3R5a/p101) subunit. | ||
| P48736 | PK3CG_HUMAN | ATP + 1-phosphatidyl-1D-myo-inositol 4,5- bisphosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,4,5- trisphosphate. | Heterodimer of a catalytic subunit (PIK3CG/p120) and a regulatory (PIK3R5a/p101) subunit. | ||
| P23727 | P85A_BOVIN | Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunits. Interacts with phosphorylated LAT, LAX1, TRAT1 and LIME1 upon TCR and/or BCR activation. Interacts with CBLB. The SH2 domains interact with the YTHM motif of phosphorylated INSR in vitro. Also interacts with tyrosine-phosphorylated IGF1R in vitro. Interacts with CD28 and CD3Z upon T-cell activation. Interacts with SOCS7. Interacts with IRS1 and phosphorylated IRS4. Interacts with NISCH, RUFY3 and HCST (By similarity). | |||
| P27986 | P85A_HUMAN | Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunits. Interacts with phosphorylated TOM1L1. Interacts with phosphorylated LIME1 upon TCR and/or BCR activation. Interacts with SOCS7. Interacts with RUFY3 (By similarity). Interacts with phosphorylated LAT, LAX1 and TRAT1 upon TCR activation. Interacts with CBLB. Interacts with HIV-1 Nef to activate the Nef associated p21-activated kinase (PAK). This interaction depends on the C- terminus of both proteins and leads to increased production of HIV. Interacts with HCV NS5A. The SH2 domains interact with the YTHM motif of phosphorylated INSR in vitro. Also interacts with tyrosine-phosphorylated IGF1R in vitro. Interacts with CD28 and CD3Z upon T-cell activation. Interacts with IRS1 and phosphorylated IRS4, as well as with NISCH and HCST. |
| KEGG Pathways | Map code | Pathways | E.C. |
|---|---|---|
| MAP00562 | Inositol phosphate metabolism | |
| MAP04070 | Phosphatidylinositol signaling system |
| Compound table | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Cofactors | Substrates | Products | Intermediates | ||||||||
| KEGG-id | C00305 | C00002 | C04637 | C00008 | C05981 | ||||||
| E.C. | |||||||||||
| Compound | Magnesium | ATP | 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | ADP | 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate | ||||||
| Type | divalent metal (Ca2+, Mg2+) | amine group,nucleotide | carbohydrate,lipid,phosphate group/phosphate ion | amine group,nucleotide | carbohydrate,lipid,phosphate group/phosphate ion | ||||||
| ChEBI |
18420 18420 |
15422 15422 |
16761 16761 |
||||||||
| PubChem |
888 888 |
5957 5957 |
6022 6022 |
||||||||
| 1e7uA01 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1e7vA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1e8wA01 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1e8xA01 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1e90A01 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1qmmA01 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1e8yA01 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1e8zA01 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1he8A01 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1e7uA02 |
|
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|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1e7vA02 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1e8wA02 |
|
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|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1e8xA02 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1e90A02 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1qmmA02 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1e8yA02 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1e8zA02 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1he8A02 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1e7uA03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1e7vA03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1e8wA03 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1e8xA03 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1e90A03 |
|
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|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1qmmA03 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1e8yA03 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1e8zA03 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1he8A03 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1e7uA04 |
|
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Unbound | Unbound | Analogue:KWT | Unbound | Unbound | |
| 1e7vA04 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1e8wA04 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1e8xA04 |
|
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Unbound | Bound:ATP | Unbound | Unbound | Unbound | |
| 1e90A04 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1qmmA04 |
|
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Unbound | Bound:ATP | Unbound | Unbound | Unbound | |
| 1e8yA04 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1e8zA04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1he8A04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1e7uA05 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1e7vA05 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1e8wA05 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1e8xA05 |
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Analogue:2x_LU | Unbound | Unbound | Unbound | Unbound | |
| 1e90A05 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1qmmA05 |
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Analogue:2x_LU | Unbound | Unbound | Unbound | Unbound | |
| 1e8yA05 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1e8zA05 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1he8A05 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1pnjA |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2pniA |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1phtA |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1pksA |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1pktA |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1pbwA |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1pbwB |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1oo3A |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1oo4A |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2pnaA |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2pnbA |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1bfiA |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1bfjA |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1qadA |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1h9oA |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1picA |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| Reference for Active-site residues | ||
|---|---|---|
| resource | references | E.C. |
| (See comments) | ||
| Active-site residues | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| PDB | Catalytic residues | Cofactor-binding residues | Modified residues | Main-chain involved in catalysis | Comment | |||||
| 1e7uA01 |
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| 1e7vA01 |
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| 1e8wA01 |
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| 1e8xA01 |
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| 1e90A01 |
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| 1qmmA01 |
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| 1e8yA01 |
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| 1e8zA01 |
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| 1he8A01 |
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| 1e7uA02 |
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mutant R505A | ||||
| 1e7vA02 |
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mutant R505A | ||||
| 1e8wA02 |
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mutant R505A | ||||
| 1e8xA02 |
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mutant R505A | ||||
| 1e90A02 |
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mutant R505A | ||||
| 1qmmA02 |
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mutant R505A | ||||
| 1e8yA02 |
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mutant P1M | ||||
| 1e8zA02 |
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mutant P1M | ||||
| 1he8A02 |
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mutant R505A | ||||
| 1e7uA03 |
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| 1e7vA03 |
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| 1e8wA03 |
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| 1e8xA03 |
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| 1e90A03 |
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| 1qmmA03 |
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| 1e8yA03 |
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| 1e8zA03 |
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| 1he8A03 |
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| 1e7uA04 |
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SER 806;LYS 807;LYS 833 | ASP 836(Magnesium binding) | |||
| 1e7vA04 |
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SER 806;LYS 807;LYS 833 | ASP 836(Magnesium binding) | |||
| 1e8wA04 |
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SER 806;LYS 807;LYS 833 | ASP 836(Magnesium binding) | |||
| 1e8xA04 |
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SER 806;LYS 807;LYS 833 | ASP 836(Magnesium binding) | |||
| 1e90A04 |
|
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SER 806;LYS 807;LYS 833 | ASP 836(Magnesium binding) | |||
| 1qmmA04 |
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SER 806;LYS 807;LYS 833 | ASP 836(Magnesium binding) | |||
| 1e8yA04 |
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SER 806;LYS 807;LYS 833 | ASP 836(Magnesium binding) | |||
| 1e8zA04 |
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SER 806;LYS 807;LYS 833 | ASP 836(Magnesium binding) | |||
| 1he8A04 |
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SER 806;LYS 807;LYS 833 | ASP 836(Magnesium binding) | |||
| 1e7uA05 |
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HIS 948;ASP 950 | ASN 951;ASP 964(Magnesium binding) | |||
| 1e7vA05 |
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HIS 948;ASP 950 | ASN 951;ASP 964(Magnesium binding) | |||
| 1e8wA05 |
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HIS 948;ASP 950 | ASN 951;ASP 964(Magnesium binding) | |||
| 1e8xA05 |
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HIS 948;ASP 950 | ASN 951;ASP 964(Magnesium binding) | |||
| 1e90A05 |
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HIS 948;ASP 950 | ASN 951;ASP 964(Magnesium binding) | |||
| 1qmmA05 |
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HIS 948;ASP 950 | ASN 951;ASP 964(Magnesium binding) | |||
| 1e8yA05 |
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HIS 948;ASP 950 | ASN 951;ASP 964(Magnesium binding) | |||
| 1e8zA05 |
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HIS 948;ASP 950 | ASN 951;ASP 964(Magnesium binding) | |||
| 1he8A05 |
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HIS 948;ASP 950 | ASN 951;ASP 964(Magnesium binding) | |||
| 1pnjA |
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| 2pniA |
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| 1phtA |
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| 1pksA |
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| 1pktA |
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| 1pbwA |
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| 1pbwB |
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| 1oo3A |
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| 1oo4A |
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| 2pnaA |
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| 2pnbA |
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| 1bfiA |
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| 1bfjA |
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| 1qadA |
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| 1h9oA |
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| 1picA |
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| References for Catalytic Mechanism | ||
|---|---|---|
| References | Sections | No. of steps in catalysis |
|
[29]
|
p.315 | |
|
[46]
|
p.428-429 | |
| References | |
|---|---|
| [1] | |
| Resource | |
| Comments | STRUCTURE BY NMR OF 314-431 |
| Medline ID | 92357146 |
| PubMed ID | 1323062 |
| Journal | Nature |
| Year | 1992 |
| Volume | 358 |
| Pages | 684-7 |
| Authors | Booker GW, Breeze AL, Downing AK, Panayotou G, Gout I, Waterfield MD, Campbell ID |
| Title | Structure of an SH2 domain of the p85 alpha subunit of phosphatidylinositol-3-OH kinase. |
| Related PDB | 2pna 2pnb |
| Related UniProtKB | P23727 |
| [2] | |
| Resource | |
| Comments | CIRCULAR DICHROISM AND FLUORESCENCE SPECTROSCOPY |
| Medline ID | 93049176 |
| PubMed ID | 1330535 |
| Journal | EMBO J |
| Year | 1992 |
| Volume | 11 |
| Pages | 4261-72 |
| Authors | Panayotou G, Bax B, Gout I, Federwisch M, Wroblowski B, Dhand R, Fry MJ, Blundell TL, Wollmer A, Waterfield MD |
| Title | Interaction of the p85 subunit of PI 3-kinase and its N-terminal SH2 domain with a PDGF receptor phosphorylation site: structural features and analysis of conformational changes. |
| Related PDB | |
| Related UniProtKB | P23727 |
| [3] | |
| Resource | |
| Comments | STRUCTURE BY NMR OF 1-84 |
| Medline ID | 93272320 |
| PubMed ID | 7684655 |
| Journal | Cell |
| Year | 1993 |
| Volume | 73 |
| Pages | 813-22 |
| Authors | Booker GW, Gout I, Downing AK, Driscoll PC, Boyd J, Waterfield MD, Campbell ID |
| Title | Solution structure and ligand-binding site of the SH3 domain of the p85 alpha subunit of phosphatidylinositol 3-kinase. |
| Related PDB | 1pnj 2pni |
| Related UniProtKB | P23727 |
| [4] | |
| Resource | |
| Comments | STRUCTURE BY NMR OF 1-79 |
| Medline ID | 93208889 |
| PubMed ID | 7681364 |
| Journal | Cell |
| Year | 1993 |
| Volume | 72 |
| Pages | 945-52 |
| Authors | Koyama S, Yu H, Dalgarno DC, Shin TB, Zydowsky LD, Schreiber SL |
| Title | Structure of the PI3K SH3 domain and analysis of the SH3 family. |
| Related PDB | 1pks 1pkt |
| Related UniProtKB | P27986 |
| [5] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 115-298 |
| Medline ID | 97121392 |
| PubMed ID | 8962058 |
| Journal | Proc Natl Acad Sci U S A |
| Year | 1996 |
| Volume | 93 |
| Pages | 14373-8 |
| Authors | Musacchio A, Cantley LC, Harrison SC |
| Title | Crystal structure of the breakpoint cluster region-homology domain from phosphoinositide 3-kinase p85 alpha subunit. |
| Related PDB | 1pbw |
| Related UniProtKB | P27986 |
| [6] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 324-434 |
| Medline ID | 96185448 |
| PubMed ID | 8599763 |
| Journal | Nat Struct Biol |
| Year | 1996 |
| Volume | 3 |
| Pages | 364-74 |
| Authors | Nolte RT, Eck MJ, Schlessinger J, Shoelson SE, Harrison SC |
| Title | Crystal structure of the PI 3-kinase p85 amino-terminal SH2 domain and its phosphopeptide complexes. |
| Related PDB | |
| Related UniProtKB | P27986 |
| [7] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 1-85 |
| Medline ID | 96196433 |
| PubMed ID | 8648629 |
| Journal | J Mol Biol |
| Year | 1996 |
| Volume | 257 |
| Pages | 632-43 |
| Authors | Liang J, Chen JK, Schreiber ST, Clardy J |
| Title | Crystal structure of P13K SH3 domain at 20 angstroms resolution. |
| Related PDB | 1pht |
| Related UniProtKB | P27986 |
| [8] | |
| Resource | |
| Comments | STRUCTURE BY NMR OF 617-724 |
| Medline ID | 96312955 |
| PubMed ID | 8670861 |
| Journal | EMBO J |
| Year | 1996 |
| Volume | 15 |
| Pages | 3579-89 |
| Authors | Breeze AL, Kara BV, Barratt DG, Anderson M, Smith JC, Luke RW, Best JR, Cartlidge SA |
| Title | Structure of a specific peptide complex of the carboxy-terminal SH2 domain from the p85 alpha subunit of phosphatidylinositol 3-kinase. |
| Related PDB | 1pic |
| Related UniProtKB | P27986 |
| [9] | |
| Resource | |
| Comments | STRUCTURE BY NMR OF 321-434 |
| Medline ID | 97110350 |
| PubMed ID | 8952511 |
| Journal | Biochemistry |
| Year | 1996 |
| Volume | 35 |
| Pages | 15570-81 |
| Authors | Gunther UL, Liu Y, Sanford D, Bachovchin WW, Schaffhausen B |
| Title | NMR analysis of interactions of a phosphatidylinositol 3'-kinase SH2 domain with phosphotyrosine peptides reveals interdependence of major binding sites. |
| Related PDB | |
| Related UniProtKB | P23727 |
| [10] | |
| Resource | |
| Comments | STRUCTURE BY NMR OF 91-104 |
| Medline ID | 97121261 |
| PubMed ID | 8961927 |
| Journal | Biochemistry |
| Year | 1996 |
| Volume | 35 |
| Pages | 15646-53 |
| Authors | Renzoni DA, Pugh DJ, Siligardi G, Das P, Morton CJ, Rossi C, Waterfield MD, Campbell ID, Ladbury JE |
| Title | Structural and thermodynamic characterization of the interaction of the SH3 domain from Fyn with the proline-rich binding site on the p85 subunit of PI3-kinase. |
| Related PDB | |
| Related UniProtKB | P27986 |
| [11] | |
| Resource | |
| Comments | VARIANT ILE-326 |
| Medline ID | 97184306 |
| PubMed ID | 9032108 |
| Journal | Diabetes |
| Year | 1997 |
| Volume | 46 |
| Pages | 494-501 |
| Authors | Hansen T, Andersen CB, Echwald SM, Urhammer SA, Clausen JO, Vestergaard H, Owens D, Hansen L, Pedersen O |
| Title |
Identification of a common amino acid polymorphism in the p85alpha regulatory subunit of phosphatidylinositol 3-kinase: effects on glucose disappearance constant, |
| Related PDB | |
| Related UniProtKB | P27986 |
| [12] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9034995 |
| Journal | J Rheumatol |
| Year | 1997 |
| Volume | 24 |
| Pages | 341-8 |
| Authors | Jackson JK, Lauener R, Duronio V, Burt HM |
| Title | The involvement of phosphatidylinositol 3-kinase in crystal induced human neutrophil activation. |
| Related PDB | |
| Related UniProtKB | |
| [13] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9391111 |
| Journal | Proc Natl Acad Sci U S A |
| Year | 1997 |
| Volume | 94 |
| Pages | 13820-5 |
| Authors | Li Z, Wahl MI, Eguinoa A, Stephens LR, Hawkins PT, Witte ON |
| Title | Phosphatidylinositol 3-kinase-gamma activates Bruton's tyrosine kinase in concert with Src family kinases. |
| Related PDB | |
| Related UniProtKB | |
| [14] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9488453 |
| Journal | Mol Cell Biol |
| Year | 1998 |
| Volume | 18 |
| Pages | 1379-87 |
| Authors | Yu J, Zhang Y, McIlroy J, Rordorf-Nikolic T, Orr GA, Backer JM |
| Title | Regulation of the p85/p110 phosphatidylinositol 3'-kinase: stabilization and inhibition of the p110alpha catalytic subunit by the p85 regulatory subunit. |
| Related PDB | |
| Related UniProtKB | |
| [15] | |
| Resource | |
| Comments | STRUCTURE BY NMR OF 614-724 |
| Medline ID | 98173872 |
| PubMed ID | 9512716 |
| Journal | J Mol Biol |
| Year | 1998 |
| Volume | 276 |
| Pages | 461-78 |
| Authors | Siegal G, Davis B, Kristensen SM, Sankar A, Linacre J, Stein RC, Panayotou G, Waterfield MD, Driscoll PC |
| Title | Solution structure of the C-terminal SH2 domain of the p85 alpha regulatory subunit of phosphoinositide 3-kinase. |
| Related PDB | 1bfi 1bfj |
| Related UniProtKB | P23727 |
| [16] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9461588 |
| Journal | J Biol Chem |
| Year | 1998 |
| Volume | 273 |
| Pages | 3994-4000 |
| Authors | von Willebrand M, Williams S, Saxena M, Gilman J, Tailor P, Jascur T, Amarante-Mendes GP, Green DR, Mustelin T |
| Title | Modification of phosphatidylinositol 3-kinase SH2 domain binding properties by Abl- or Lck-mediated tyrosine phosphorylation at Tyr-688. |
| Related PDB | |
| Related UniProtKB | |
| [17] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9804776 |
| Journal | J Biol Chem |
| Year | 1998 |
| Volume | 273 |
| Pages | 30199-203 |
| Authors | Yu J, Wjasow C, Backer JM |
| Title |
Regulation of the p85/p110alpha phosphatidylinositol 3'-kinase. |
| Related PDB | |
| Related UniProtKB | |
| [18] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9677338 |
| Journal | Biochem J |
| Year | 1998 |
| Volume | 333 |
| Pages | 757-63 |
| Authors | Hellyer NJ, Cheng K, Koland JG |
| Title | ErbB3 (HER3) interaction with the p85 regulatory subunit of phosphoinositide 3-kinase. |
| Related PDB | |
| Related UniProtKB | |
| [19] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9551103 |
| Journal | J Mol Biol |
| Year | 1998 |
| Volume | 276 |
| Pages | 657-67 |
| Authors | Guijarro JI, Morton CJ, Plaxco KW, Campbell ID, Dobson CM |
| Title | Folding kinetics of the SH3 domain of PI3 kinase by real-time NMR combined with optical spectroscopy. |
| Related PDB | |
| Related UniProtKB | |
| [20] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9539718 |
| Journal | Proc Natl Acad Sci U S A |
| Year | 1998 |
| Volume | 95 |
| Pages | 4224-8 |
| Authors | Guijarro JI, Sunde M, Jones JA, Campbell ID, Dobson CM |
| Title | Amyloid fibril formation by an SH3 domain. |
| Related PDB | |
| Related UniProtKB | |
| [21] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9593201 |
| Journal | Proteins |
| Year | 1998 |
| Volume | 31 |
| Pages | 309-19 |
| Authors | Knapp S, Mattson PT, Christova P, Berndt KD, Karshikoff A, Vihinen M, Smith CI, Ladenstein R |
| Title | Thermal unfolding of small proteins with SH3 domain folding pattern. |
| Related PDB | |
| Related UniProtKB | |
| [22] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9765155 |
| Journal | Science |
| Year | 1998 |
| Volume | 282 |
| Pages | 293-6 |
| Authors | Bondeva T, Pirola L, Bulgarelli-Leva G, Rubio I, Wetzker R, Wymann MP |
| Title | Bifurcation of lipid and protein kinase signals of PI3Kgamma to the protein kinases PKB and MAPK. |
| Related PDB | |
| Related UniProtKB | |
| [23] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10525402 |
| Journal | J Mol Biol |
| Year | 1999 |
| Volume | 292 |
| Pages | 763-70 |
| Authors | Hoedemaeker FJ, Siegal G, Roe SM, Driscoll PC, Abrahams JP |
| Title | Crystal structure of the C-terminal SH2 domain of the p85alpha regulatory subunit of phosphoinositide 3-kinase: an SH2 domain mimicking its own substrate. |
| Related PDB | |
| Related UniProtKB | |
| [24] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10358930 |
| Journal | Chem Phys Lipids |
| Year | 1999 |
| Volume | 98 |
| Pages | 79-86 |
| Authors | Katada T, Kurosu H, Okada T, Suzuki T, Tsujimoto N, Takasuga S, Kontani K, Hazeki O, Ui M |
| Title | Synergistic activation of a family of phosphoinositide 3-kinase via G-protein coupled and tyrosine kinase-related receptors. |
| Related PDB | |
| Related UniProtKB | |
| [25] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10022824 |
| Journal | EMBO J |
| Year | 1999 |
| Volume | 18 |
| Pages | 815-21 |
| Authors | Jimenez JL, Guijarro JI, Orlova E, Zurdo J, Dobson CM, Sunde M, Saibil HR |
| Title | Cryo-electron microscopy structure of an SH3 amyloid fibril and model of the molecular packing. |
| Related PDB | |
| Related UniProtKB | |
| [26] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10212202 |
| Journal | J Biol Chem |
| Year | 1999 |
| Volume | 274 |
| Pages | 12323-32 |
| Authors | Harpur AG, Layton MJ, Das P, Bottomley MJ, Panayotou G, Driscoll PC, Waterfield MD |
| Title | Intermolecular interactions of the p85alpha regulatory subunit of phosphatidylinositol 3-kinase. |
| Related PDB | |
| Related UniProtKB | |
| [27] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10356365 |
| Journal | Mol Cell Biol Res Commun |
| Year | 1999 |
| Volume | 1 |
| Pages | 153-7 |
| Authors | Beeton CA, Das P, Waterfield MD, Shepherd PR |
| Title | The SH3 and BH domains of the p85alpha adapter subunit play a critical role in regulating class Ia phosphoinositide 3-kinase function. |
| Related PDB | |
| Related UniProtKB | |
| [28] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10358071 |
| Journal | J Biol Chem |
| Year | 1999 |
| Volume | 274 |
| Pages | 17152-8 |
| Authors | Krugmann S, Hawkins PT, Pryer N, Braselmann S |
| Title | Characterizing the interactions between the two subunits of the p101/p110gamma phosphoinositide 3-kinase and their role in the activation of this enzyme by G beta gamma subunits. |
| Related PDB | |
| Related UniProtKB | |
| [29] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10580505 |
| Journal | Nature |
| Year | 1999 |
| Volume | 402 |
| Pages | 313-20 |
| Authors | Walker EH, Perisic O, Ried C, Stephens L, Williams RL |
| Title |
Structural insights into phosphoinositide 3-kinase catalysis and signalling. |
| Related PDB | |
| Related UniProtKB | |
| [30] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10835283 |
| Journal | J Mol Biol |
| Year | 2000 |
| Volume | 299 |
| Pages | 771-88 |
| Authors | Kristensen SM, Siegal G, Sankar A, Driscoll PC |
| Title | Backbone dynamics of the C-terminal SH2 domain of the p85alpha subunit of phosphoinositide 3-kinase: effect of phosphotyrosine-peptide binding and characterization of slow conformational exchange processes. |
| Related PDB | |
| Related UniProtKB | |
| [31] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10967104 |
| Journal | J Biol Chem |
| Year | 2000 |
| Volume | 275 |
| Pages | 36450-6 |
| Authors | King TR, Fang Y, Mahon ES, Anderson DH |
| Title | Using a phage display library to identify basic residues in A-Raf required to mediate binding to the Src homology 2 domains of the p85 subunit of phosphatidylinositol 3'-kinase. |
| Related PDB | |
| Related UniProtKB | |
| [32] | |
| Resource | |
| Comments |
VARIANT INSULIN RESISTANCE GLN-409, |
| Medline ID | 20230645 |
| PubMed ID | 10768093 |
| Journal | Diabetologia |
| Year | 2000 |
| Volume | 43 |
| Pages | 321-31 |
| Authors | Baynes KC, Beeton CA, Panayotou G, Stein R, Soos M, Hansen T, Simpson H, O'Rahilly S, Shepherd PR, Whitehead JP |
| Title | Natural variants of human p85 alpha phosphoinositide 3-kinase in severe insulin resistance: a novel variant with impaired insulin-stimulated lipid kinase activity. |
| Related PDB | |
| Related UniProtKB | P27986 |
| [33] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11123912 |
| Journal | Biochemistry |
| Year | 2000 |
| Volume | 39 |
| Pages | 15860-9 |
| Authors | Weber T, Schaffhausen B, Liu Y, Gunther UL |
| Title | NMR structure of the N-SH2 of the p85 subunit of phosphoinositide 3-kinase complexed to a doubly phosphorylated peptide reveals a second phosphotyrosine binding site. |
| Related PDB | |
| Related UniProtKB | |
| [34] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10861385 |
| Journal | Biopolymers |
| Year | 2000 |
| Volume | 57 |
| Pages | 208-17 |
| Authors | Okishio N, Nagai M, Fukuda R, Nagatomo S, Kitagawa T |
| Title |
Interactions of phosphatidylinositol 3-kinase Src homology 3 domain with its ligand peptide studied by absorption, |
| Related PDB | |
| Related UniProtKB | |
| [35] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 11136978 |
| Journal | Cell |
| Year | 2000 |
| Volume | 103 |
| Pages | 931-43 |
| Authors | Pacold ME, Suire S, Perisic O, Lara-Gonzalez S, Davis CT, Walker EH, Hawkins PT, Stephens L, Eccleston JF, Williams RL |
| Title | Crystal structure and functional analysis of Ras binding to its effector phosphoinositide 3-kinase gamma. |
| Related PDB | 1he8 |
| Related UniProtKB | |
| [36] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11067939 |
| Journal | J Immunol |
| Year | 2000 |
| Volume | 165 |
| Pages | 5798-806 |
| Authors | Tudan C, Jackson JK, Blanis L, Pelech SL, Burt HM |
| Title | Inhibition of TNF-alpha-induced neutrophil apoptosis by crystals of calcium pyrophosphate dihydrate is mediated by the extracellular signal-regulated kinase and phosphatidylinositol 3-kinase/Akt pathways up-stream of caspase 3. |
| Related PDB | |
| Related UniProtKB | |
| [37] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 11090628 |
| Journal | Mol Cell |
| Year | 2000 |
| Volume | 6 |
| Pages | 909-19 |
| Authors | Walker EH, Pacold ME, Perisic O, Stephens L, Hawkins PT, Wymann MP, Williams RL |
| Title |
Structural determinants of phosphoinositide 3-kinase inhibition by wortmannin, |
| Related PDB | 1e7u 1e7v 1e8w 1e8x 1e8y 1e8z 1e90 1qmm |
| Related UniProtKB | |
| [38] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11418612 |
| Journal | J Biol Chem |
| Year | 2001 |
| Volume | 276 |
| Pages | 36174-82 |
| Authors | Gelkop S, Babichev Y, Isakov N |
| Title | T cell activation induces direct binding of the Crk adapter protein to the regulatory subunit of phosphatidylinositol 3-kinase (p85) via a complex mechanism involving the Cbl protein. |
| Related PDB | |
| Related UniProtKB | |
| [39] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11567151 |
| Journal | Acta Crystallogr D Biol Crystallogr |
| Year | 2001 |
| Volume | 57 |
| Pages | 1397-404 |
| Authors | Pauptit RA, Dennis CA, Derbyshire DJ, Breeze AL, Weston SA, Rowsell S, Murshudov GN |
| Title | NMR trial models: experiences with the colicin immunity protein Im7 and the p85alpha C-terminal SH2-peptide complex. |
| Related PDB | 1h9o |
| Related UniProtKB | |
| [40] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11141062 |
| Journal | Biochemistry |
| Year | 2001 |
| Volume | 40 |
| Pages | 119-29 |
| Authors | Okishio N, Tanaka T, Fukuda R, Nagai M |
| Title | Role of the conserved acidic residue Asp21 in the structure of phosphatidylinositol 3-kinase Src homology 3 domain: circular dichroism and nuclear magnetic resonance studies. |
| Related PDB | |
| Related UniProtKB | |
| [41] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11747457 |
| Journal | Biochemistry |
| Year | 2001 |
| Volume | 40 |
| Pages | 15797-804 |
| Authors | Okishio N, Tanaka T, Nagai M, Fukuda R, Nagatomo S, Kitagawa T |
| Title | Identification of tyrosine residues involved in ligand recognition by the phosphatidylinositol 3-kinase Src homology 3 domain: circular dichroism and UV resonance Raman studies. |
| Related PDB | |
| Related UniProtKB | |
| [42] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11478864 |
| Journal | J Mol Biol |
| Year | 2001 |
| Volume | 311 |
| Pages | 325-40 |
| Authors | Zurdo J, Guijarro JI, Jimenez JL, Saibil HR, Dobson CM |
| Title | Dependence on solution conditions of aggregation and amyloid formation by an SH3 domain. |
| Related PDB | |
| Related UniProtKB | |
| [43] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11401516 |
| Journal | Biochem Biophys Res Commun |
| Year | 2001 |
| Volume | 282 |
| Pages | 691-6 |
| Authors | Kirsch C, Wetzker R, Klinger R |
| Title | Anionic phospholipids are involved in membrane targeting of PI 3-kinase gamma. |
| Related PDB | |
| Related UniProtKB | |
| [44] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12367534 |
| Journal | J Mol Biol |
| Year | 2002 |
| Volume | 322 |
| Pages | 1147-58 |
| Authors | Ventura S, Lacroix E, Serrano L |
| Title | Insights into the origin of the tendency of the PI3-SH3 domain to form amyloid fibrils. |
| Related PDB | |
| Related UniProtKB | |
| [45] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11986952 |
| Journal | Leukemia |
| Year | 2002 |
| Volume | 16 |
| Pages | 894-901 |
| Authors | Jucker M, Sudel K, Horn S, Sickel M, Wegner W, Fiedler W, Feldman RA |
| Title | Expression of a mutated form of the p85alpha regulatory subunit of phosphatidylinositol 3-kinase in a Hodgkin's lymphoma-derived cell line (CO). |
| Related PDB | |
| Related UniProtKB | |
| [46] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12151228 |
| Journal | Trends Biochem Sci |
| Year | 2002 |
| Volume | 27 |
| Pages | 426-32 |
| Authors | Djordjevic S, Driscoll PC |
| Title | Structural insight into substrate specificity and regulatory mechanisms of phosphoinositide 3-kinases. |
| Related PDB | |
| Related UniProtKB | |
| [47] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12515556 |
| Journal | Biochemistry |
| Year | 2003 |
| Volume | 42 |
| Pages | 208-16 |
| Authors | Okishio N, Tanaka T, Fukuda R, Nagai M |
| Title | Differential ligand recognition by the Src and phosphatidylinositol 3-kinase Src homology 3 domains: circular dichroism and ultraviolet resonance Raman studies. |
| Related PDB | |
| Related UniProtKB | |
| [48] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12629217 |
| Journal | Proc Natl Acad Sci U S A |
| Year | 2003 |
| Volume | 100 |
| Pages | 3275-80 |
| Authors | Fu Z, Aronoff-Spencer E, Backer JM, Gerfen GJ |
| Title | The structure of the inter-SH2 domain of class IA phosphoinositide 3-kinase determined by site-directed spin labeling EPR and homology modeling. |
| Related PDB | |
| Related UniProtKB | |
| [49] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 15046599 |
| Journal | Biochem Soc Trans |
| Year | 2004 |
| Volume | 32 |
| Pages | 320-5 |
| Authors | Koyasu S |
| Title | Role of class IA phosphoinositide 3-kinase in B lymphocyte development and functions. |
| Related PDB | |
| Related UniProtKB | |
| Comments |
|---|
|
This enzyme is composed of a catalytic subunit and a regulatory subunit.
### The regulatory subunit of PI3-kinase, ### Although the catalytic mechanism of this enzyme is not so clear, If there is no general base, Moreover, Instead of the basic resdiues, Taken together, (1) Asp950 acts as a general base to deprotonate the acceptor group of the substrate, (2) The activated acceptor group makes a nuclepophilic attack on the transferred group, (3) Lys807 may stabilize the negative charge on the transferred group, (4) Finally, |
| Created | Updated |
|---|---|
| 2004-03-18 | 2009-02-26 |