DB code: M00186

CATH domain 3.20.20.140 : TIM Barrel Catalytic domain
-.-.-.- :
-.-.-.- : Catalytic domain
2.40.50.140 : OB fold (Dihydrolipoamide Acetyltransferase, E2P)
-.-.-.- :
3.30.420.10 : Nucleotidyltransferase; domain 5 Catalytic domain
-.-.-.- :
1.10.1110.10 : DNA Polymerase III; Chain A
3.40.50.300 : Rossmann fold Catalytic domain
1.20.272.10 : Zinc Finger, Delta Prime; domain 3
1.10.8.60 : Helicase, Ruva Protein; domain 3
-.-.-.- :
-.-.-.- :
3.40.50.300 : Rossmann fold
1.20.272.20 : Zinc Finger, Delta Prime; domain 3
1.10.8.60 : Helicase, Ruva Protein; domain 3
3.40.50.300 : Rossmann fold
1.10.8.10 : Helicase, Ruva Protein; domain 3
1.20.272.10 : Zinc Finger, Delta Prime; domain 3
3.40.50.10220 : Rossmann fold
3.40.50.10110 : Rossmann fold
3.10.150.10 : DNA Polymerase III; Chain A, domain 2
3.10.150.10 : DNA Polymerase III; Chain A, domain 2
3.10.150.10 : DNA Polymerase III; Chain A, domain 2
E.C. 2.7.7.7 3.1.11.1 3.6.1.3
CSA 1j53
M-CSA 1j53
MACiE

CATH domain Related DB codes (homologues)
3.40.50.300 : Rossmann fold S00527 S00547 S00548 S00550 S00554 S00555 S00671 S00672 S00676 S00680 S00682 S00913 S00914 S00301 S00302 S00303 S00304 S00307 S00308 S00305 S00306 S00309 S00310 S00311 M00114 M00199 D00129 D00130 D00540
2.40.50.140 : OB fold (Dihydrolipoamide Acetyltransferase, E2P) M00220 T00050 D00291 D00294 T00254
3.20.20.140 : TIM Barrel S00231 S00232 D00673 D00675 D00801 D00873 M00030 M00225 M00226
3.30.420.10 : Nucleotidyltransferase; domain 5 M00206 T00252 M00019 M00020 M00055 M00135 M00146 M00166 M00173 M00175

Uniprot Enzyme Name
UniprotKB Protein name Synonyms Contains RefSeq Pfam
P10443 DNA polymerase III subunit alpha
EC 2.7.7.7
None NP_414726.1 (Protein)
NC_000913.2 (DNA/RNA sequence)
YP_488486.1 (Protein)
NC_007779.1 (DNA/RNA sequence)
PF07733 (DNA_pol3_alpha)
PF02811 (PHP)
PF01336 (tRNA_anti)
[Graphical View]
P03007 DNA polymerase III subunit epsilon
EC 2.7.7.7
None NP_414751.1 (Protein)
NC_000913.2 (DNA/RNA sequence)
YP_488512.1 (Protein)
NC_007779.1 (DNA/RNA sequence)
PF00929 (RNase_T)
[Graphical View]
P0ABS8 DNA polymerase III subunit theta
EC 2.7.7.7
None NP_416356.1 (Protein)
NC_000913.2 (DNA/RNA sequence)
YP_490104.1 (Protein)
NC_007779.1 (DNA/RNA sequence)
PF06440 (DNA_pol3_theta)
[Graphical View]
P06710 DNA polymerase III subunit tau
EC 2.7.7.7
DNA polymerase III subunit gamma
NP_415003.1 (Protein)
NC_000913.2 (DNA/RNA sequence)
YP_488761.1 (Protein)
NC_007779.1 (DNA/RNA sequence)
PF12169 (DNA_pol3_gamma3)
PF12168 (DNA_pol3_tau_4)
PF12170 (DNA_pol3_tau_5)
[Graphical View]
P28630 DNA polymerase III subunit delta
EC 2.7.7.7
None NP_415173.1 (Protein)
NC_000913.2 (DNA/RNA sequence)
YP_488931.1 (Protein)
NC_007779.1 (DNA/RNA sequence)
PF06144 (DNA_pol3_delta)
[Graphical View]
P28631 DNA polymerase III subunit delta''
EC 2.7.7.7
None NP_415617.1 (Protein)
NC_000913.2 (DNA/RNA sequence)
YP_489367.1 (Protein)
NC_007779.1 (DNA/RNA sequence)
PF09115 (DNApol3-delta_C)
[Graphical View]
P28632 DNA polymerase III subunit psi
EC 2.7.7.7
None NP_418789.1 (Protein)
NC_000913.2 (DNA/RNA sequence)
YP_492501.1 (Protein)
NC_007779.1 (DNA/RNA sequence)
PF03603 (DNA_III_psi)
[Graphical View]
P28905 DNA polymerase III subunit chi
EC 2.7.7.7
None NP_418680.1 (Protein)
NC_000913.2 (DNA/RNA sequence)
YP_492397.1 (Protein)
NC_007779.1 (DNA/RNA sequence)
PF04364 (DNA_pol3_chi)
[Graphical View]
P0A988 DNA polymerase III subunit beta
EC 2.7.7.7
None NP_418156.1 (Protein)
NC_000913.2 (DNA/RNA sequence)
YP_491734.1 (Protein)
NC_007779.1 (DNA/RNA sequence)
PF00712 (DNA_pol3_beta)
PF02767 (DNA_pol3_beta_2)
PF02768 (DNA_pol3_beta_3)
[Graphical View]

KEGG enzyme name
DNA-directed DNA polymerase
(EC 2.7.7.7 )
DNA polymerase I
(EC 2.7.7.7 )
DNA polymerase II
(EC 2.7.7.7 )
DNA polymerase III
(EC 2.7.7.7 )
DNA polymerase alpha
(EC 2.7.7.7 )
DNA polymerase beta
(EC 2.7.7.7 )
DNA polymerase gamma
(EC 2.7.7.7 )
DNA nucleotidyltransferase (DNA-directed)
(EC 2.7.7.7 )
DNA nucleotidyltransferase (DNA-directed)
(EC 2.7.7.7 )
deoxyribonucleate nucleotidyltransferase
(EC 2.7.7.7 )
deoxynucleate polymerase
(EC 2.7.7.7 )
deoxyribonucleic acid duplicase
(EC 2.7.7.7 )
deoxyribonucleic acid polymerase
(EC 2.7.7.7 )
deoxyribonucleic duplicase
(EC 2.7.7.7 )
deoxyribonucleic polymerase
(EC 2.7.7.7 )
deoxyribonucleic polymerase I
(EC 2.7.7.7 )
DNA duplicase
(EC 2.7.7.7 )
DNA nucleotidyltransferase
(EC 2.7.7.7 )
DNA polymerase
(EC 2.7.7.7 )
DNA replicase
(EC 2.7.7.7 )
DNA-dependent DNA polymerase
(EC 2.7.7.7 )
duplicase
(EC 2.7.7.7 )
Klenow fragment
(EC 2.7.7.7 )
sequenase
(EC 2.7.7.7 )
Taq DNA polymerase
(EC 2.7.7.7 )
Taq Pol I
(EC 2.7.7.7 )
Tca DNA polymerase
(EC 2.7.7.7 )
exodeoxyribonuclease I
(EC 3.1.11.1 )
Escherichia coli exonuclease I
(EC 3.1.11.1 )
E. coli exonuclease I
(EC 3.1.11.1 )
exonuclease I
(EC 3.1.11.1 )
adenosinetriphosphatase
(EC 3.6.1.3 )
adenylpyrophosphatase
(EC 3.6.1.3 )
ATP monophosphatase
(EC 3.6.1.3 )
triphosphatase
(EC 3.6.1.3 )
ATPase
(EC 3.6.1.3 )
SV40 T-antigen
(EC 3.6.1.3 )
adenosine 5'-triphosphatase
(EC 3.6.1.3 )
ATP hydrolase
(EC 3.6.1.3 )
ATPase
(EC 3.6.1.3 )
complex V (mitochondrial electron transport)
(EC 3.6.1.3 )
(Ca2+ + Mg2+)-ATPase
(EC 3.6.1.3 )
HCO3--ATPase
(EC 3.6.1.3 )
adenosine triphosphatase
(EC 3.6.1.3 )

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P10443 DPO3A_ECOLI Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). The DNA polymerase holoenzyme is a complex that contains 10 different types of subunits. These subunits are organized into 3 functionally essential subassemblies: the pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The pol III core (subunits alpha,epsilon and theta) contains the polymerase and the 3''-5'' exonuclease proofreading activities. The polymerase is tethered to the template via the sliding clamp processivity factor. The clamp-loading complex assembles the beta processivity factor onto the primer template and plays a central role in the organization and communication at the replication fork. This complex contains delta, delta'', psi and chi, and copies of either or both of two different dnaX proteins, gamma and tau. The composition of the holoenzyme is, therefore: (alpha,epsilon,theta)[2]-(gamma/tau)[3]-delta,delta'', psi,chi- beta[4]. Cytoplasm.
P03007 DPO3E_ECOLI Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). The DNA polymerase holoenzyme is a complex that contains 10 different types of subunits. These subunits are organized into 3 functionally essential subassemblies: the pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The pol III core (subunits alpha,epsilon and theta) contains the polymerase and the 3''-5'' exonuclease proofreading activities. The polymerase is tethered to the template via the sliding clamp processivity factor. The clamp-loading complex assembles the beta processivity factor onto the primer template and plays a central role in the organization and communication at the replication fork. This complex contains delta, delta'', psi and chi, and copies of either or both of two different dnaX proteins, gamma and tau. The composition of the holoenzyme is, therefore: (alpha,epsilon,theta)[2]-(gamma/tau)[3]-delta,delta'', psi,chi- beta[4]. Binds 2 divalent metal cations. Magnesium or manganese.
P0ABS8 HOLE_ECOLI Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). The DNA polymerase holoenzyme is a complex that contains 10 different types of subunits. These subunits are organized into 3 functionally essential subassemblies: the pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The pol III core (subunits alpha,epsilon and theta) contains the polymerase and the 3''-5'' exonuclease proofreading activities. The polymerase is tethered to the template via the sliding clamp processivity factor. The clamp-loading complex assembles the beta processivity factor onto the primer template and plays a central role in the organization and communication at the replication fork. This complex contains delta, delta'', psi and chi, and copies of either or both of two different dnaX proteins, gamma and tau. The composition of the holoenzyme is, therefore: (alpha,epsilon,theta)[2]-(gamma/tau)[3]-delta,delta'', psi,chi- beta[4].
P06710 DPO3X_ECOLI Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). The DNA polymerase holoenzyme is a complex that contains 10 different types of subunits. These subunits are organized into 3 functionally essential subassemblies: the pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The pol III core (subunits alpha,epsilon and theta) contains the polymerase and the 3''-5'' exonuclease proofreading activities. The polymerase is tethered to the template via the sliding clamp processivity factor. The clamp-loading complex assembles the beta processivity factor onto the primer template and plays a central role in the organization and communication at the replication fork. This complex contains delta, delta'', psi and chi, and copies of either or both of two different dnaX proteins, gamma and tau. The composition of the holoenzyme is, therefore: (alpha,epsilon,theta)[2]-(gamma/tau)[3]-delta,delta'', psi,chi- beta[4].
P28630 HOLA_ECOLI Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). The DNA polymerase holoenzyme is a complex that contains 10 different types of subunits. These subunits are organized into 3 functionally essential subassemblies: the pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The pol III core (subunits alpha,epsilon and theta) contains the polymerase and the 3''-5'' exonuclease proofreading activities. The polymerase is tethered to the template via the sliding clamp processivity factor. The clamp-loading complex assembles the beta processivity factor onto the primer template and plays a central role in the organization and communication at the replication fork. This complex contains delta, delta'', psi and chi, and copies of either or both of two different dnaX proteins, gamma and tau. The composition of the holoenzyme is, therefore: (alpha,epsilon,theta)[2]-(gamma/tau)[3]-delta,delta'', psi,chi- beta[4].
P28631 HOLB_ECOLI Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). The DNA polymerase holoenzyme is a complex that contains 10 different types of subunits. These subunits are organized into 3 functionally essential subassemblies: the pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The pol III core (subunits alpha,epsilon and theta) contains the polymerase and the 3''-5'' exonuclease proofreading activities. The polymerase is tethered to the template via the sliding clamp processivity factor. The clamp-loading complex assembles the beta processivity factor onto the primer template and plays a central role in the organization and communication at the replication fork. This complex contains delta, delta'', psi and chi, and copies of either or both of two different dnaX proteins, gamma and tau. The composition of the holoenzyme is, therefore: (alpha,epsilon,theta)[2]-(gamma/tau)[3]-delta,delta'', psi,chi- beta[4].
P28632 HOLD_ECOLI Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). The DNA polymerase holoenzyme is a complex that contains 10 different types of subunits. These subunits are organized into 3 functionally essential subassemblies: the pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The pol III core (subunits alpha,epsilon and theta) contains the polymerase and the 3''-5'' exonuclease proofreading activities. The polymerase is tethered to the template via the sliding clamp processivity factor. The clamp-loading complex assembles the beta processivity factor onto the primer template and plays a central role in the organization and communication at the replication fork. This complex contains delta, delta'', psi and chi, and copies of either or both of two different dnaX proteins, gamma and tau. The composition of the holoenzyme is, therefore: (alpha,epsilon,theta)[2]-(gamma/tau)[3]-delta,delta'', psi,chi- beta[4].
P28905 HOLC_ECOLI Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). The DNA polymerase holoenzyme is a complex that contains 10 different types of subunits. These subunits are organized into 3 functionally essential subassemblies: the pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The pol III core (subunits alpha,epsilon and theta) contains the polymerase and the 3''-5'' exonuclease proofreading activities. The polymerase is tethered to the template via the sliding clamp processivity factor. The clamp-loading complex assembles the beta processivity factor onto the primer template and plays a central role in the organization and communication at the replication fork. This complex contains delta, delta'', psi and chi, and copies of either or both of two different dnaX proteins, gamma and tau. The composition of the holoenzyme is, therefore: (alpha,epsilon,theta)[2]-(gamma/tau)[3]-delta,delta'', psi,chi- beta[4].
P0A988 DPO3B_ECOLI Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). The DNA polymerase holoenzyme is a complex that contains 10 different types of subunits. These subunits are organized into 3 functionally essential subassemblies: the pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The pol III core (subunits alpha,epsilon and theta) contains the polymerase and the 3''-5'' exonuclease proofreading activities. The polymerase is tethered to the template via the sliding clamp processivity factor. The clamp-loading complex assembles the beta processivity factor onto the primer template and plays a central role in the organization and communication at the replication fork. This complex contains delta, delta'', psi and chi, and copies of either or both of two different dnaX proteins, gamma and tau. The composition of the holoenzyme is, therefore: (alpha,epsilon,theta)[2]-(gamma/tau)[3]-delta,delta'', psi,chi- beta[4]. The beta chain is a homodimer when not associated with the other components. Cytoplasm.

KEGG Pathways
Map code Pathways E.C.
MAP00230 Purine metabolism 2.7.7.7 3.6.1.3
MAP00240 Pyrimidine metabolism 2.7.7.7

Compound table
Cofactors Substrates Products Intermediates
KEGG-id C02148 C00677 C00039 C00039 C00001 C00002 C00013 C00039 C00039 C01150 C00008 C00009
E.C. 2.7.7.7
3.1.11.1
2.7.7.7
2.7.7.7
3.1.11.1
3.1.11.1
3.6.1.3
3.6.1.3
2.7.7.7
2.7.7.7
3.1.11.1
3.1.11.1
3.6.1.3
3.6.1.3
Compound Divalent metal Deoxynucleoside triphosphate DNA(n) DNA(n+1) H2O ATP Pyrophosphate DNA(n+1) DNA(n) 5'-Phosphomononucleotides ADP Orthophosphate
Type divalent metal (Ca2+, Mg2+) nucleotide nucleic acids nucleic acids H2O amine group,nucleotide phosphate group/phosphate ion nucleic acids nucleic acids amine group,nucleotide phosphate group/phosphate ion
ChEBI 15377
 15377
 		15422
 15422
 		29888
 29888
 		16761
 16761
 		26078
 26078
PubChem 22247451
 962
 22247451
 962
 		5957
 5957
 		1023
 21961011
 1023
 21961011
 		6022
 6022
 		1004
 22486802
 1004
 22486802
1j53A Bound:2x_MN Unbound Unbound Unbound Unbound Unbound Unbound Analogue:TMP_2100 Bound:TMP_2000 Unbound Unbound
1j54A Bound:2x_MN Unbound Unbound Unbound Unbound Unbound Unbound Unbound Bound:TMP_2000 Unbound Unbound
1du2A Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2ae9A Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1jr3A01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Analogue:SO4
1jr3B01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Analogue:SO4
1jr3C01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Analogue:SO4
1xxhB01 Unbound Unbound Unbound Unbound Analogue:ATG Unbound Unbound Unbound Unbound Unbound Unbound
1xxhC01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Bound:PO4
1xxhD01 Unbound Unbound Unbound Unbound Analogue:ATG Unbound Unbound Unbound Unbound Unbound Unbound
1xxhG01 Unbound Unbound Unbound Unbound Analogue:ATG Unbound Unbound Unbound Unbound Unbound Unbound
1xxhH01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Bound:PO4
1xxhI01 Unbound Unbound Unbound Unbound Analogue:ATG Unbound Unbound Unbound Unbound Unbound Unbound
1xxiB01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Bound:ADP Unbound
1xxiC01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Bound:PO4
1xxiD01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Bound:ADP Unbound
1xxiG01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxiH01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxiI01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Bound:ADP Unbound
1njfA01 Unbound Unbound Unbound Unbound Analogue:ATG Unbound Unbound Unbound Unbound Unbound Unbound
1njfB01 Unbound Unbound Unbound Unbound Analogue:ATG Unbound Unbound Unbound Unbound Unbound Unbound
1njfC01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Bound:ADP Unbound
1njfD01 Unbound Unbound Unbound Unbound Analogue:ATG Unbound Unbound Unbound Unbound Unbound Unbound
1njgA01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Analogue:SO4
1njgB01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Analogue:SO4
1jr3A02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1jr3B02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1jr3C02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxhB02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxhC02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxhD02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxhG02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxhH02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxhI02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxiB02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxiC02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxiD02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxiG02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxiH02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxiI02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1jr3A03 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1jr3B03 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1jr3C03 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxhB03 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxhC03 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxhD03 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxhG03 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxhH03 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxhI03 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxiB03 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxiC03 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxiD03 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxiG03 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxiH03 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxiI03 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1njfA02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1njfB02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1njfC02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1njfD02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1njgA02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1njgB02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1jr3D01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxhA01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxhF01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxiA01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxiF01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1jqjC01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1jqjD01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1jqlB Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1jr3D02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxhA02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxhF02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxiA02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxiF02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1jqjC02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1jqjD02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1jr3D03 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxhA03 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxhF03 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxiA03 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxiF03 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1jqjC03 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1jqjD03 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1a5tA01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1jr3E01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxhE01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxhJ01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxiE01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxiJ01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1a5tA02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1jr3E02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxhE02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxhJ02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxiE02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxiJ02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1a5tA03 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1jr3E03 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxhE03 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxhJ03 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxiE03 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1xxiJ03 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1em8B Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1em8D Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1em8A Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1em8C Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1jqjA01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1jqjB01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1jqlA01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1mmiA01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1mmiB01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1ok7A01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1ok7B01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1unnA01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1unnB01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2polA01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2polB01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1jqjA02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1jqjB02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1jqlA02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1mmiA02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1mmiB02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1ok7A02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1ok7B02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1unnA02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1unnB02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2polA02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2polB02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1jqjA03 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1jqjB03 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1jqlA03 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1mmiA03 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1mmiB03 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1ok7A03 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1ok7B03 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1unnA03 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1unnB03 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2polA03 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2polB03 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1j53A GLU 14;HIS 162 ASP 12;GLU 14;ASP 167(divalent metal binding)
1j54A GLU 14;HIS 162 ASP 12;GLU 14;ASP 167(divalent metal binding)
1du2A
2ae9A
1jr3A01 CYS 64;CYS 73;CYS 76;CYS 79(Zinc binding)
1jr3B01 CYS 2064;CYS 73;CYS 76;CYS 79(Zinc binding)
1jr3C01 CYS 64;CYS 73;CYS 76;CYS 79(Zinc binding)
1xxhB01 CYS 64;CYS 73;CYS 76;CYS 79(Zinc binding)
1xxhC01 CYS 64;CYS 73;CYS 76;CYS 79(Zinc binding)
1xxhD01 CYS 64;CYS 73;CYS 76;CYS 79(Zinc binding)
1xxhG01 CYS 64;CYS 73;CYS 76;CYS 79(Zinc binding)
1xxhH01 CYS 64;CYS 73;CYS 76;CYS 79(Zinc binding)
1xxhI01 CYS 64;CYS 73;CYS 76;CYS 79(Zinc binding)
1xxiB01 CYS 64;CYS 73;CYS 76;CYS 79(Zinc binding)
1xxiC01 CYS 64;CYS 73;CYS 76;CYS 79(Zinc binding)
1xxiD01 CYS 64;CYS 73;CYS 76;CYS 79(Zinc binding)
1xxiG01 CYS 64;CYS 73;CYS 76;CYS 79(Zinc binding)
1xxiH01 CYS 64;CYS 73;CYS 76;CYS 79(Zinc binding)
1xxiI01 CYS 64;CYS 73;CYS 76;CYS 79(Zinc binding)
1njfA01 CYS 64;CYS 73;CYS 76;CYS 79(Zinc binding)
1njfB01 CYS 64;CYS 73;CYS 76;CYS 79(Zinc binding)
1njfC01 CYS 64;CYS 73;CYS 76;CYS 79(Zinc binding)
1njfD01 CYS 64;CYS 73;CYS 76;CYS 79(Zinc binding)
1njgA01 CYS 64;CYS 73;CYS 76;CYS 79(Zinc binding)
1njgB01 CYS 64;CYS 73;CYS 76;CYS 79(Zinc binding)
1jr3A02
1jr3B02
1jr3C02
1xxhB02
1xxhC02
1xxhD02
1xxhG02
1xxhH02
1xxhI02
1xxiB02
1xxiC02
1xxiD02
1xxiG02
1xxiH02
1xxiI02
1jr3A03
1jr3B03
1jr3C03
1xxhB03
1xxhC03
1xxhD03
1xxhG03
1xxhH03
1xxhI03
1xxiB03
1xxiC03
1xxiD03
1xxiG03
1xxiH03
1xxiI03
1njfA02
1njfB02
1njfC02
1njfD02
1njgA02
1njgB02
1jr3D01
1xxhA01
1xxhF01
1xxiA01
1xxiF01
1jqjC01
1jqjD01
1jqlB
1jr3D02
1xxhA02
1xxhF02
1xxiA02
1xxiF02
1jqjC02
1jqjD02
1jr3D03
1xxhA03
1xxhF03
1xxiA03
1xxiF03
1jqjC03
1jqjD03
1a5tA01 CYS 50;CYS 59;CYS 62;CYS 65(Zinc binding)
1jr3E01 CYS 50;CYS 59;CYS 62;CYS 65(Zinc binding)
1xxhE01 CYS 50;CYS 59;CYS 62;CYS 65(Zinc binding)
1xxhJ01 CYS 50;CYS 59;CYS 62;CYS 65(Zinc binding)
1xxiE01 CYS 50;CYS 59;CYS 62;CYS 65(Zinc binding)
1xxiJ01 CYS 50;CYS 59;CYS 62;CYS 65(Zinc binding)
1a5tA02
1jr3E02
1xxhE02
1xxhJ02
1xxiE02
1xxiJ02
1a5tA03
1jr3E03
1xxhE03
1xxhJ03
1xxiE03
1xxiJ03
1em8B
1em8D
1em8A
1em8C
1jqjA01
1jqjB01
1jqlA01
1mmiA01
1mmiB01
1ok7A01
1ok7B01
1unnA01
1unnB01
2polA01
2polB01
1jqjA02
1jqjB02
1jqlA02
1mmiA02
1mmiB02
1ok7A02
1ok7B02
1unnA02
1unnB02
2polA02
2polB02
1jqjA03
1jqjB03
1jqlA03
1mmiA03
1mmiB03
1ok7A03
1ok7B03
1unnA03
1unnB03
2polA03
2polB03

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[34]
Fig.5, p.540-543

References
[1]
Resource
Comments
Medline ID
PubMed ID 7118945
Journal J Biol Chem
Year 1982
Volume 257
Pages 12310-5
Authors Johanson KO, McHenry CS
Title The beta subunit of the DNA polymerase III holoenzyme becomes inaccessible to antibody after formation of an initiation complex with primed DNA.
Related PDB
Related UniProtKB
[2]
Resource
Comments
Medline ID
PubMed ID 3037519
Journal Proc Natl Acad Sci U S A
Year 1987
Volume 84
Pages 4389-92
Authors Maki H, Kornberg A
Title Proofreading by DNA polymerase III of Escherichia coli depends on cooperative interaction of the polymerase and exonuclease subunits.
Related PDB
Related UniProtKB
[3]
Resource
Comments
Medline ID
PubMed ID 3283128
Journal J Biol Chem
Year 1988
Volume 263
Pages 6570-8
Authors Maki H, Maki S, Kornberg A
Title DNA Polymerase III holoenzyme of Escherichia coli. IV. The holoenzyme is an asymmetric dimer with twin active sites.
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID 2243096
Journal J Biol Chem
Year 1990
Volume 265
Pages 20356-63
Authors Griep MA, McHenry CS
Title Dissociation of the DNA polymerase III holoenzyme beta 2 subunits is accompanied by conformational change at distal cysteines 333.
Related PDB
Related UniProtKB
[5]
Resource
Comments
Medline ID
PubMed ID 1918028
Journal J Biol Chem
Year 1991
Volume 266
Pages 19127-30
Authors McHenry CS
Title DNA polymerase III holoenzyme. Components, structure, and mechanism of a true replicative complex.
Related PDB
Related UniProtKB
[6]
Resource
Comments REVIEW.
Medline ID 92246902
PubMed ID 1575709
Journal Bioessays
Year 1992
Volume 14
Pages 105-11
Authors O'Donnell M
Title Accessory protein function in the DNA polymerase III holoenzyme from E. coli.
Related PDB
Related UniProtKB P28631
[7]
Resource
Comments
Medline ID
PubMed ID 1740452
Journal J Biol Chem
Year 1992
Volume 267
Pages 4045-53
Authors Zechner EL, Wu CA, Marians KJ
Title Coordinated leading- and lagging-strand synthesis at the Escherichia coli DNA replication fork. II. Frequency of primer synthesis and efficiency of primer utilization control Okazaki fragment size.
Related PDB
Related UniProtKB
[8]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS)
Medline ID 92257585
PubMed ID 1349852
Journal Cell
Year 1992
Volume 69
Pages 425-37
Authors Kong XP, Onrust R, O'Donnell M, Kuriyan J
Title Three-dimensional structure of the beta subunit of E. coli DNA polymerase III holoenzyme: a sliding DNA clamp.
Related PDB 2pol
Related UniProtKB P00583
[9]
Resource
Comments CHARACTERIZATION.
Medline ID 93280137
PubMed ID 8505304
Journal J Biol Chem
Year 1993
Volume 268
Pages 11766-72
Authors Onrust R, O'Donnell M
Title DNA polymerase III accessory proteins. II. Characterization of delta and delta'.
Related PDB
Related UniProtKB P28631
[10]
Resource
Comments
Medline ID
PubMed ID 7903401
Journal J Mol Biol
Year 1993
Volume 234
Pages 915-25
Authors Kuriyan J, O'Donnell M
Title Sliding clamps of DNA polymerases.
Related PDB
Related UniProtKB
[11]
Resource
Comments
Medline ID
PubMed ID 8505305
Journal J Biol Chem
Year 1993
Volume 268
Pages 11779-84
Authors Xiao H, Dong Z, O'Donnell M
Title DNA polymerase III accessory proteins. IV. Characterization of chi and psi.
Related PDB
Related UniProtKB
[12]
Resource
Comments
Medline ID
PubMed ID 8001157
Journal Cell
Year 1994
Volume 79
Pages 1233-43
Authors Krishna TS, Kong XP, Gary S, Burgers PM, Kuriyan J
Title Crystal structure of the eukaryotic DNA polymerase processivity factor PCNA.
Related PDB
Related UniProtKB
[13]
Resource
Comments
Medline ID
PubMed ID 8300534
Journal J Bacteriol
Year 1994
Volume 176
Pages 815-21
Authors Slater SC, Lifsics MR, O'Donnell M, Maurer R
Title holE, the gene coding for the theta subunit of DNA polymerase III of Escherichia coli: characterization of a holE mutant and comparison with a dnaQ (epsilon-subunit) mutant.
Related PDB
Related UniProtKB
[14]
Resource
Comments
Medline ID
PubMed ID 7768937
Journal J Biol Chem
Year 1995
Volume 270
Pages 13358-65
Authors Naktinis V, Onrust R, Fang L, O'Donnell M
Title Assembly of a chromosomal replication machine: two DNA polymerases, a clamp loader, and sliding clamps in one holoenzyme particle. II. Intermediate complex between the clamp loader and its clamp.
Related PDB
Related UniProtKB
[15]
Resource
Comments
Medline ID
PubMed ID 7768938
Journal J Biol Chem
Year 1995
Volume 270
Pages 13366-77
Authors Onrust R, Finkelstein J, Turner J, Naktinis V, O'Donnell M
Title Assembly of a chromosomal replication machine: two DNA polymerases, a clamp loader, and sliding clamps in one holoenzyme particle. III. Interface between two polymerases and the clamp loader.
Related PDB
Related UniProtKB
[16]
Resource
Comments
Medline ID
PubMed ID 7768939
Journal J Biol Chem
Year 1995
Volume 270
Pages 13378-83
Authors Xiao H, Naktinis V, O'Donnell M
Title Assembly of a chromosomal replication machine: two DNA polymerases, a clamp loader, and sliding clamps in one holoenzyme particle. IV. ATP-binding site mutants identify the clamp loader.
Related PDB
Related UniProtKB
[17]
Resource
Comments
Medline ID
PubMed ID 7478986
Journal Nucleic Acids Res
Year 1995
Volume 23
Pages 3613-20
Authors Kelman Z, O'Donnell M
Title Structural and functional similarities of prokaryotic and eukaryotic DNA polymerase sliding clamps.
Related PDB
Related UniProtKB
[18]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
Medline ID 98028572
PubMed ID 9363942
Journal Cell
Year 1997
Volume 91
Pages 335-45
Authors Guenther B, Onrust R, Sali A, O'Donnell M, Kuriyan J
Title Crystal structure of the delta' subunit of the clamp-loader complex of E. coli DNA polymerase III.
Related PDB 1a5t
Related UniProtKB P28631
[19]
Resource
Comments
Medline ID
PubMed ID 9346941
Journal J Biol Chem
Year 1997
Volume 272
Pages 27919-30
Authors Bloom LB, Chen X, Fygenson DK, Turner J, O'Donnell M, Goodman MF
Title Fidelity of Escherichia coli DNA polymerase III holoenzyme. The effects of beta, gamma complex processivity proteins and epsilon proofreading exonuclease on nucleotide misincorporation efficiencies.
Related PDB
Related UniProtKB
[20]
Resource
Comments
Medline ID
PubMed ID 9753470
Journal Biochemistry
Year 1998
Volume 37
Pages 13807-15
Authors Terashima I, Suzuki N, Dasaradhi L, Tan CK, Downey KM, Shibutani S
Title Translesional synthesis on DNA templates containing an estrogen quinone-derived adduct: N2-(2-hydroxyestron-6-yl)-2'-deoxyguanosine and N6-(2-hydroxyestron-6-yl)-2'-deoxyadenosine.
Related PDB
Related UniProtKB
[21]
Resource
Comments
Medline ID
PubMed ID 9685491
Journal Nucleic Acids Res
Year 1998
Volume 26
Pages 3746-52
Authors Aravind L, Koonin EV
Title Phosphoesterase domains associated with DNA polymerases of diverse origins.
Related PDB
Related UniProtKB
[22]
Resource
Comments
Medline ID
PubMed ID 11101526
Journal EMBO J
Year 2000
Volume 19
Pages 6536-45
Authors Pritchard AE, Dallmann HG, Glover BP, McHenry CS
Title A novel assembly mechanism for the DNA polymerase III holoenzyme DnaX complex: association of deltadelta' with DnaX(4) forms DnaX(3)deltadelta'.
Related PDB
Related UniProtKB
[23]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 10794414
Journal Protein Sci
Year 2000
Volume 9
Pages 721-33
Authors Keniry MA, Berthon HA, Yang JY, Miles CS, Dixon NE
Title NMR solution structure of the theta subunit of DNA polymerase III from Escherichia coli.
Related PDB 1du2
Related UniProtKB
[24]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 11525729
Journal Cell
Year 2001
Volume 106
Pages 429-41
Authors Jeruzalmi D, O'Donnell M, Kuriyan J
Title Crystal structure of the processivity clamp loader gamma (gamma) complex of E. coli DNA polymerase III.
Related PDB 1jr3
Related UniProtKB
[25]
Resource
Comments
Medline ID
PubMed ID 11518714
Journal J Biol Chem
Year 2001
Volume 276
Pages 40668-79
Authors Song MS, Dallmann HG, McHenry CS
Title Carboxyl-terminal domain III of the delta' subunit of the DNA polymerase III holoenzyme binds delta.
Related PDB
Related UniProtKB
[26]
Resource
Comments
Medline ID
PubMed ID 11606586
Journal J Biol Chem
Year 2001
Volume 276
Pages 48709-15
Authors Song MS, McHenry CS
Title Carboxyl-terminal domain III of the delta' subunit of DNA polymerase III holoenzyme binds DnaX and supports cooperative DnaX complex assembly.
Related PDB
Related UniProtKB
[27]
Resource
Comments
Medline ID
PubMed ID 11572866
Journal J Biol Chem
Year 2001
Volume 276
Pages 47185-94
Authors Leu FP, O'Donnell M
Title Interplay of clamp loader subunits in opening the beta sliding clamp of Escherichia coli DNA polymerase III holoenzyme.
Related PDB
Related UniProtKB
[28]
Resource
Comments
Medline ID
PubMed ID 11279099
Journal J Biol Chem
Year 2001
Volume 276
Pages 19182-9
Authors Stewart J, Hingorani MM, Kelman Z, O'Donnell M
Title Mechanism of beta clamp opening by the delta subunit of Escherichia coli DNA polymerase III holoenzyme.
Related PDB
Related UniProtKB
[29]
Resource
Comments
Medline ID
PubMed ID 11719243
Journal Curr Biol
Year 2001
Volume 11
Pages R935-46
Authors O'Donnell M, Jeruzalmi D, Kuriyan J
Title Clamp loader structure predicts the architecture of DNA polymerase III holoenzyme and RFC.
Related PDB
Related UniProtKB
[30]
Resource
Comments
Medline ID
PubMed ID 11525728
Journal Cell
Year 2001
Volume 106
Pages 417-28
Authors Jeruzalmi D, Yurieva O, Zhao Y, Young M, Stewart J, Hingorani M, O'Donnell M, Kuriyan J
Title Mechanism of processivity clamp opening by the delta subunit wrench of the clamp loader complex of E. coli DNA polymerase III.
Related PDB 1jqj 1jql
Related UniProtKB
[31]
Resource
Comments
Medline ID
PubMed ID 11859073
Journal J Biol Chem
Year 2002
Volume 277
Pages 17334-48
Authors Bruck I, Yuzhakov A, Yurieva O, Jeruzalmi D, Skangalis M, Kuriyan J, O'Donnell M
Title Analysis of a multicomponent thermostable DNA polymerase III replicase from an extreme thermophile.
Related PDB
Related UniProtKB
[32]
Resource
Comments
Medline ID
PubMed ID 11809766
Journal J Biol Chem
Year 2002
Volume 277
Pages 13246-56
Authors Bullard JM, Pritchard AE, Song MS, Glover BP, Wieczorek A, Chen J, Janjic N, McHenry CS
Title A three-domain structure for the delta subunit of the DNA polymerase III holoenzyme delta domain III binds delta' and assembles into the DnaX complex.
Related PDB
Related UniProtKB
[33]
Resource
Comments
Medline ID
PubMed ID 11772007
Journal Biochemistry
Year 2002
Volume 41
Pages 94-110
Authors DeRose EF, Li D, Darden T, Harvey S, Perrino FW, Schaaper RM, London RE
Title Model for the catalytic domain of the proofreading epsilon subunit of Escherichia coli DNA polymerase III based on NMR structural data.
Related PDB
Related UniProtKB
[34]
Resource
Comments
Medline ID
PubMed ID 11937058
Journal Structure
Year 2002
Volume 10
Pages 535-46
Authors Hamdan S, Carr PD, Brown SE, Ollis DL, Dixon NE
Title Structural basis for proofreading during replication of the Escherichia coli chromosome.
Related PDB 1j53 1j54
Related UniProtKB
[35]
Resource
Comments
Medline ID
PubMed ID 14592985
Journal EMBO J
Year 2003
Volume 22
Pages 5883-92
Authors Bunting KA, Roe SM, Pearl LH
Title Structural basis for recruitment of translesion DNA polymerase Pol IV/DinB to the beta-clamp.
Related PDB 1unn
Related UniProtKB
[36]
Resource
Comments
Medline ID
PubMed ID 12832762
Journal Acta Crystallogr D Biol Crystallogr
Year 2003
Volume 59
Pages 1192-9
Authors Oakley AJ, Prosselkov P, Wijffels G, Beck JL, Wilce MC, Dixon NE
Title Flexibility revealed by the 1.85 A crystal structure of the beta sliding-clamp subunit of Escherichia coli DNA polymerase III.
Related PDB 1mmi
Related UniProtKB
[37]
Resource
Comments
Medline ID
PubMed ID 12623013
Journal Structure
Year 2003
Volume 11
Pages 253-63
Authors Podobnik M, Weitze TF, O'Donnell M, Kuriyan J
Title Nucleotide-induced conformational changes in an isolated Escherichia coli DNA polymerase III clamp loader subunit.
Related PDB 1njf 1njg
Related UniProtKB
[38]
Resource
Comments
Medline ID
PubMed ID 14717711
Journal Eur J Biochem
Year 2004
Volume 271
Pages 439-49
Authors Gulbis JM, Kazmirski SL, Finkelstein J, Kelman Z, O'Donnell M, Kuriyan J
Title Crystal structure of the chi:psi sub-assembly of the Escherichia coli DNA polymerase clamp-loader complex.
Related PDB 1em8
Related UniProtKB
[39]
Resource
Comments
Medline ID
PubMed ID 14610068
Journal J Biol Chem
Year 2004
Volume 279
Pages 4386-93
Authors Snyder AK, Williams CR, Johnson A, O'Donnell M, Bloom LB
Title Mechanism of loading the Escherichia coli DNA polymerase III sliding clamp: II. Uncoupling the beta and DNA binding activities of the gamma complex.
Related PDB
Related UniProtKB
[40]
Resource
Comments
Medline ID
PubMed ID 15037068
Journal J Mol Biol
Year 2004
Volume 336
Pages 1047-59
Authors Goedken ER, Levitus M, Johnson A, Bustamante C, O'Donnell M, Kuriyan J
Title Fluorescence measurements on the E.coli DNA polymerase clamp loader: implications for conformational changes during ATP and clamp binding.
Related PDB
Related UniProtKB
[41]
Resource
Comments
Medline ID
PubMed ID 14729336
Journal J Mol Biol
Year 2004
Volume 335
Pages 1187-97
Authors Burnouf DY, Olieric V, Wagner J, Fujii S, Reinbolt J, Fuchs RP, Dumas P
Title Structural and biochemical analysis of sliding clamp/ligand interactions suggest a competition between replicative and translesion DNA polymerases.
Related PDB 1ok7
Related UniProtKB
[42]
Resource
Comments
Medline ID
PubMed ID 15556993
Journal Proc Natl Acad Sci U S A
Year 2004
Volume 101
Pages 16750-5
Authors Kazmirski SL, Podobnik M, Weitze TF, O'Donnell M, Kuriyan J
Title Structural analysis of the inactive state of the Escherichia coli DNA polymerase clamp-loader complex.
Related PDB 1xxh 1xxi
Related UniProtKB
[43]
Resource
Comments
Medline ID
PubMed ID 16199579
Journal J Bacteriol
Year 2005
Volume 187
Pages 7081-9
Authors Mueller GA, Kirby TW, DeRose EF, Li D, Schaaper RM, London RE
Title Nuclear magnetic resonance solution structure of the Escherichia coli DNA polymerase III theta subunit.
Related PDB 2ae9
Related UniProtKB

Comments
This enzyme belongs to the DNA polymerase type-C family.
This DNA enzyme is composed of 10 different types of subunits.
According to the literature, these subunits are organized into 3 functionally essential subassemblies:
(a) the pol III core (subunits alpha, epsilon and theta)
(b) the clamp-loading complex (subunits delta, delta', psi and chi, and either or both of gamma and tau)
(c) the beta sliding clamp processivity factor (subunit beta)
The pol III core contains the polymerase and the 3'-5' exonuclease proofreading activities. The polymerase (a) is tethered to the template via the sliding clamp processivity factor (c).
The clamp-loading complex (b) assembles the beta processivity factor (c) onto the primer template and plays a central role in the organization and communication at the replication fork.
The composition of the enzyme is as follows:
(a) (alpha, epsilon, theta)[2]
(b) (gamma/tau)[3]-(delta, delta', psi, chi)[2]
(c) (beta[2])[2]
The roles of the subunits are as follows:
(a) (alpha, epsilon, theta)[2];
alpha subunit: A polymerase subunit.
epsilon subunit: A proofreading subunit, with 3'-5' exonuclease activity.
theta subunit: This subunit might maintain fidelity.
(b) (gamma/tau)[3]-(delta, delta', psi, chi)[2];
gamma subunit: This subunit is a short vaiant of dnaX and interacts with delta subunit to transfer the beta subunit on the DNA. Moreover, this subunit has ATPase activity.
tau subunit: This subunit is a long vaiant of dnaX, and also a scaffold to help in the dimerization of the core complex.
delta subunit: This subunit interacts with gamma subunit to transfer the beta subunit on the DNA.
delta' subunit: The function is unknown.
chi subunit: The function is unknown.
psi subunit: The function is unknown.
(c) (beta[2])[2];
beta subunit: This subunit seems to be required for initiation of replication.

Created Updated
2005-03-30 2009-02-26