DB code: M00030
| CATH domain | 2.30.40.10 : Urease, subunit C; domain 1 | |
|---|---|---|
| 3.20.20.140 : TIM Barrel | Catalytic domain | |
| 2.10.150.10 : Urease, subunit B | ||
| 3.30.280.10 : Urease; subunit A | ||
| E.C. | 3.5.1.5 | |
| CSA | 1kra | |
| M-CSA | 1kra | |
| MACiE | M0087 | |
| CATH domain | Related DB codes (homologues) |
|---|---|
| 2.10.150.10 : Urease, subunit B | M00225 M00226 |
| 2.30.40.10 : Urease, subunit C; domain 1 | D00673 D00675 D00801 D00873 M00225 M00226 |
| 3.20.20.140 : TIM Barrel | S00231 S00232 M00186 D00673 D00675 D00801 D00873 M00225 M00226 |
| 3.30.280.10 : Urease; subunit A | M00225 M00226 |
| Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | MEROPS | Pfam | RefSeq |
|---|---|---|---|---|---|
| P18314 |
Urease subunit alpha
|
EC
3.5.1.5
Urea amidohydrolase subunit alpha |
M38.982
(Metallo)
|
PF01979
(Amidohydro_1)
PF00449 (Urease_alpha) [Graphical View] |
|
| P41020 |
Urease subunit alpha
|
EC
3.5.1.5
Urea amidohydrolase subunit alpha |
M38.982
(Metallo)
|
PF01979
(Amidohydro_1)
PF00449 (Urease_alpha) [Graphical View] |
|
| P0A660 |
Urease subunit alpha
|
EC
3.5.1.5
Urea amidohydrolase subunit alpha |
M38.982
(Metallo)
|
PF01979
(Amidohydro_1)
PF00449 (Urease_alpha) [Graphical View] |
NP_216366.1
(Protein)
NC_000962.3 (DNA/RNA sequence) NP_336355.1 (Protein) NC_002755.2 (DNA/RNA sequence) YP_006515250.1 (Protein) NC_018143.1 (DNA/RNA sequence) YP_007610509.1 (Protein) NC_020559.1 (DNA/RNA sequence) |
| P18315 |
Urease subunit beta
|
EC
3.5.1.5
Urea amidohydrolase subunit beta |
M38.982
(Metallo)
|
PF00699
(Urease_beta)
[Graphical View] |
|
| P41021 |
Urease subunit beta
|
EC
3.5.1.5
Urea amidohydrolase subunit beta |
PF00699
(Urease_beta)
[Graphical View] |
||
| P0A662 |
Urease subunit beta
|
EC
3.5.1.5
Urea amidohydrolase subunit beta |
PF00699
(Urease_beta)
[Graphical View] |
NP_216365.1
(Protein)
NC_000962.3 (DNA/RNA sequence) NP_336354.1 (Protein) NC_002755.2 (DNA/RNA sequence) YP_006515249.1 (Protein) NC_018143.1 (DNA/RNA sequence) YP_007610508.1 (Protein) NC_020559.1 (DNA/RNA sequence) |
|
| P18316 |
Urease subunit gamma
|
EC
3.5.1.5
Urea amidohydrolase subunit gamma |
PF00547
(Urease_gamma)
[Graphical View] |
||
| P41022 |
Urease subunit gamma
|
EC
3.5.1.5
Urea amidohydrolase subunit gamma |
PF00547
(Urease_gamma)
[Graphical View] |
||
| P0A676 |
Urease subunit gamma
|
EC
3.5.1.5
Urea amidohydrolase subunit gamma |
PF00547
(Urease_gamma)
[Graphical View] |
NP_216364.1
(Protein)
NC_000962.3 (DNA/RNA sequence) NP_336353.1 (Protein) NC_002755.2 (DNA/RNA sequence) YP_006515248.1 (Protein) NC_018143.1 (DNA/RNA sequence) YP_007610507.1 (Protein) NC_020559.1 (DNA/RNA sequence) |
| KEGG enzyme name |
|---|
|
Urease
Urea amidohydrolase |
| UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
|---|---|---|---|---|---|
| P18314 | URE1_ENTAE | Urea + H(2)O = CO(2) + 2 NH(3). | Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme. The apoenzyme interacts with an accessory complex composed of UreD, UreF and UreG, which is required for the assembly of the nickel containing metallocenter of UreC. The UreE protein may also play a direct role as a metallochaperone in nickel transfer to the urease apoprotein. | Cytoplasm (By similarity). | Binds 2 nickel ions per subunit. |
| P41020 | URE1_BACPA | Urea + H(2)O = CO(2) + 2 NH(3). | Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme. | Cytoplasm (By similarity). | Binds 2 nickel ions per subunit. |
| P0A660 | URE1_MYCTU | Urea + H(2)O = CO(2) + 2 NH(3). | Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme. | Cytoplasm (By similarity). | Binds 2 nickel ions per subunit. |
| P18315 | URE2_ENTAE | Urea + H(2)O = CO(2) + 2 NH(3). | Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme. The apoenzyme interacts with an accessory complex composed of UreD, UreF and UreG, which is required for the assembly of the nickel containing metallocenter of UreC. The UreE protein may also play a direct role as a metallochaperone in nickel transfer to the urease apoprotein. | Cytoplasm (By similarity). | |
| P41021 | URE2_BACPA | Urea + H(2)O = CO(2) + 2 NH(3). | Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme. | Cytoplasm (By similarity). | |
| P0A662 | URE2_MYCTU | Urea + H(2)O = CO(2) + 2 NH(3). | Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme. | Cytoplasm (By similarity). | |
| P18316 | URE3_ENTAE | Urea + H(2)O = CO(2) + 2 NH(3). | Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme. The apoenzyme interacts with an accessory complex composed of UreD, UreF and UreG, which is required for the assembly of the nickel containing metallocenter of UreC. The UreE protein may also play a direct role as a metallochaperone in nickel transfer to the urease apoprotein. | Cytoplasm (By similarity). | |
| P41022 | URE3_BACPA | Urea + H(2)O = CO(2) + 2 NH(3). | Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme. | Cytoplasm (By similarity). | |
| P0A676 | URE3_MYCTU | Urea + H(2)O = CO(2) + 2 NH(3). | Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme. | Cytoplasm (By similarity). |
| KEGG Pathways | Map code | Pathways | E.C. |
|---|---|---|
| MAP00220 | Urea cycle and metabolism of amino groups | |
| MAP00230 | Purine metabolism | |
| MAP00791 | Atrazine degradation |
| Compound table | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cofactors | Substrates | Products | Intermediates | |||||||||
| KEGG-id | C00291 | C00086 | C00001 | C00011 | C00014 | I00127 | C01563 | |||||
| E.C. | ||||||||||||
| Compound | Nickel | Urea | H2O | CO2 | NH3 | Diaminohydroxymethanolate | Carbamate | |||||
| Type | heavy metal | amide group,amine group | H2O | others | amine group,organic ion | |||||||
| ChEBI |
28112 28112 |
16199 48376 16199 48376 |
15377 15377 |
16526 16526 |
16134 16134 |
|||||||
| PubChem |
935 935 |
1176 1176 |
22247451 962 22247451 962 |
280 280 |
222 222 |
|||||||
| 1a5kC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1a5lC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1a5mC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1a5nC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1a5oC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ef2A01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ejrC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ejsC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ejtC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ejuC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ejvC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ejwC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ejxC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1fwaC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1fwbC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1fwcC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1fwdC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1fweC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1fwfC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1fwgC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1fwhC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1fwiC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1fwjC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1kauC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1kraC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1krbC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1krcC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2kauC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 3kauC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ie7C01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1s3tC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ubpC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2ubpC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 3ubpC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 4ubpC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1a5kC02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1a5lC02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1a5mC02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1a5nC02 |
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Bound:2x_NI | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1a5oC02 |
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Bound:2x_NI | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ef2A02 |
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Analogue:2x_MN | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ejrC02 |
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Bound:2x_NI | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ejsC02 |
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Bound:2x_NI | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ejtC02 |
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Bound:2x_NI | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ejuC02 |
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Bound:2x_NI | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ejvC02 |
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Bound:2x_NI | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ejwC02 |
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Bound:2x_NI | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ejxC02 |
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Bound:2x_NI | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1fwaC02 |
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Bound:2x_NI | Unbound | Unbound | Unbound | Unbound | Intermediate-analogue:CO3 | |
| 1fwbC02 |
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Bound:2x_NI | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1fwcC02 |
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Bound:2x_NI | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1fwdC02 |
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Bound:2x_NI | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1fweC02 |
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Bound:2x_NI | Unbound | Unbound | Unbound | Unbound | Intermediate-analogue:HAE | |
| 1fwfC02 |
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Bound:2x_NI | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1fwgC02 |
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Bound:2x_NI | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1fwhC02 |
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Bound:2x_NI | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1fwiC02 |
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Bound:_NI | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1fwjC02 |
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Bound:2x_NI | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1kauC02 |
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Bound:2x_NI | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1kraC02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1krbC02 |
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Bound:2x_NI | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1krcC02 |
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Bound:2x_NI | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2kauC02 |
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Bound:2x_NI | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 3kauC02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ie7C02 |
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Bound:2x_NI | Unbound | Unbound | Unbound | Transition-state-analogue:PO4 | Unbound | |
| 1s3tC02 |
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Bound:2x_NI | Analogue:BO3 | Bound:HOH_604 | Unbound | Unbound | Unbound | Unbound |
| 1ubpC02 |
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Bound:2x_NI | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2ubpC02 |
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Bound:2x_NI | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 3ubpC02 |
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Bound:2x_NI | Unbound | Unbound | Unbound | Transition-state-analogue:2PA | Unbound | |
| 4ubpC02 |
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Bound:2x_NI | Unbound | Unbound | Unbound | Unbound | Intermediate-analogue:HAE | |
| 1a5kB |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1a5lB |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1a5mB |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1a5nB |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1a5oB |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ef2B |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ejrB |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ejsB |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ejtB |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ejuB |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ejvB |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ejwB |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ejxB |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1fwaB |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1fwbB |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1fwcB |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1fwdB |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1fweB |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1fwfB |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1fwgB |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1fwhB |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1fwiB |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1fwjB |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1kauB |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1kraB |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1krbB |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1krcB |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2kauB |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 3kauB |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ie7B |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1s3tB |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ubpB |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2ubpB |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 3ubpB |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 4ubpB |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1a5kA |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1a5lA |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1a5mA |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1a5nA |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1a5oA |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ef2C |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ejrA |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ejsA |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ejtA |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ejuA |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ejvA |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ejwA |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ejxA |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1fwaA |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1fwbA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1fwcA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1fwdA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1fweA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1fwfA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1fwgA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1fwhA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1fwiA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1fwjA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1kauA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1kraA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1krbA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1krcA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2kauA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 3kauA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ie7A |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1s3tA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ubpA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2ubpA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 3ubpA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 4ubpA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2fvhA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2fvhB |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2fvhC |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| Reference for Active-site residues | ||
|---|---|---|
| resource | references | E.C. |
| Swiss-prot;P18314, P41020, P69996 & literature [17], [22], [31] | ||
| Active-site residues | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| PDB | Catalytic residues | Cofactor-binding residues | Modified residues | Main-chain involved in catalysis | Comment | |||||
| 1a5kC01 |
|
|
|
|
|
|||||
| 1a5lC01 |
|
|
|
|
|
|||||
| 1a5mC01 |
|
|
|
|
|
|||||
| 1a5nC01 |
|
|
|
|
|
|||||
| 1a5oC01 |
|
|
|
|
|
|||||
| 1ef2A01 |
|
|
|
|
|
|||||
| 1ejrC01 |
|
|
|
|
|
|||||
| 1ejsC01 |
|
|
|
|
|
|||||
| 1ejtC01 |
|
|
|
|
|
|||||
| 1ejuC01 |
|
|
|
|
|
|||||
| 1ejvC01 |
|
|
|
|
|
|||||
| 1ejwC01 |
|
|
|
|
|
|||||
| 1ejxC01 |
|
|
|
|
|
|||||
| 1fwaC01 |
|
|
|
|
|
|||||
| 1fwbC01 |
|
|
|
|
|
|||||
| 1fwcC01 |
|
|
|
|
|
|||||
| 1fwdC01 |
|
|
|
|
|
|||||
| 1fweC01 |
|
|
|
|
|
|||||
| 1fwfC01 |
|
|
|
|
|
|||||
| 1fwgC01 |
|
|
|
|
|
|||||
| 1fwhC01 |
|
|
|
|
|
|||||
| 1fwiC01 |
|
|
|
|
|
|||||
| 1fwjC01 |
|
|
|
|
|
|||||
| 1kauC01 |
|
|
|
|
|
|||||
| 1kraC01 |
|
|
|
|
|
|||||
| 1krbC01 |
|
|
|
|
|
|||||
| 1krcC01 |
|
|
|
|
|
|||||
| 2kauC01 |
|
|
|
|
|
|||||
| 3kauC01 |
|
|
|
|
|
|||||
| 1ie7C01 |
|
|
|
|
|
|||||
| 1s3tC01 |
|
|
|
|
|
|||||
| 1ubpC01 |
|
|
|
|
|
|||||
| 2ubpC01 |
|
|
|
|
|
|||||
| 3ubpC01 |
|
|
|
|
|
|||||
| 4ubpC01 |
|
|
|
|
|
|||||
| 1a5kC02 |
|
|
|
|
|
HIS 219;ASP 221;HIS 320;ARG 336 | ;HIS 246;HIS 272(Nickel-1);HIS 134;HIS 136;ASP 360(Nickel-2) | mutant K217E | ||
| 1a5lC02 |
|
|
|
|
|
HIS 219;ASP 221; ; | ;HIS 246;HIS 272(Nickel-1);HIS 134;HIS 136;ASP 360(Nickel-2) | mutant K217C, C319A, invisible 309-338 | ||
| 1a5mC02 |
|
|
|
|
|
HIS 219;ASP 221;HIS 320;ARG 336 | ;HIS 246;HIS 272(Nickel-1);HIS 134;HIS 136;ASP 360(Nickel-2) | mutant K217A | ||
| 1a5nC02 |
|
|
|
|
|
HIS 219;ASP 221;;ARG 336 | ;HIS 246;HIS 272(Nickel-1);HIS 134;HIS 136;ASP 360(Nickel-2) | mutant K217A, invisible 316-330 | ||
| 1a5oC02 |
|
|
|
|
|
HIS 219;ASP 221;HIS 320;ARG 336 | ;HIS 246;HIS 272(Nickel-1);HIS 134;HIS 136;ASP 360(Nickel-2) | mutant K217C, C319A | ||
| 1ef2A02 |
|
|
|
|
|
HIS 1219;ASP 1221;HIS 1320;ARG 1336 | KCX 1217(Nickel-1 & -2);HIS 1246;HIS 1272(Nickel-1);HIS 1134;HIS 1136;ASP 1360(Nickel-2) | KCX 1217(Carbamylated LYS) | ||
| 1ejrC02 |
|
|
|
|
|
HIS 1219; ; ;ARG 1336 | KCX 1217(Nickel-1 & -2);HIS 1246;HIS 1272(Nickel-1);HIS 1134;HIS 1136;ASP 1360(Nickel-2) | KCX 1217(Carbamylated LYS) | mutant D1221A, invisible 1318-1330 | |
| 1ejsC02 |
|
|
|
|
|
;ASP 1221;HIS 1320;ARG 1336 | KCX 1217(Nickel-1 & -2);HIS 1246;HIS 1272(Nickel-1);HIS 1134;HIS 1136;ASP 1360(Nickel-2) | KCX 1217(Carbamylated LYS) | mutant H1219N | |
| 1ejtC02 |
|
|
|
|
|
;ASP 1221;HIS 1320;ARG 1336 | KCX 1217(Nickel-1 & -2);HIS 1246;HIS 1272(Nickel-1);HIS 1134;HIS 1136;ASP 1360(Nickel-2) | KCX 1217(Carbamylated LYS) | mutant H1219Q | |
| 1ejuC02 |
|
|
|
|
|
HIS 1219;ASP 1221; ;ARG 1336 | KCX 1217(Nickel-1 & -2);HIS 1246;HIS 1272(Nickel-1);HIS 1134;HIS 1136;ASP 1360(Nickel-2) | KCX 1217(Carbamylated LYS) | mutant H1320N, invisible 1318-1330 | |
| 1ejvC02 |
|
|
|
|
|
HIS 1219;ASP 1221; ;ARG 1336 | KCX 1217(Nickel-1 & -2);HIS 1246;HIS 1272(Nickel-1);HIS 1134;HIS 1136;ASP 1360(Nickel-2) | KCX 1217(Carbamylated LYS) | mutant H1320Q, invisible 1318-1330 | |
| 1ejwC02 |
|
|
|
|
|
HIS 1219;ASP 1221;HIS 1320;ARG 1336 | KCX 1217(Nickel-1 & -2);HIS 1246;HIS 1272(Nickel-1);HIS 1134;HIS 1136;ASP 1360(Nickel-2) | KCX 1217(Carbamylated LYS) | ||
| 1ejxC02 |
|
|
|
|
|
HIS 1219;ASP 1221; ;ARG 1336 | KCX 1217(Nickel-1 & -2);HIS 1246;HIS 1272(Nickel-1);HIS 1134;HIS 1136;ASP 1360(Nickel-2) | KCX 1217(Carbamylated LYS) | invisible 1320-1329 | |
| 1fwaC02 |
|
|
|
|
|
HIS 219;ASP 221;HIS 320;ARG 336 | KCX 217(Nickel-1 & -2);HIS 246;HIS 272(Nickel-1);HIS 134;HIS 136;ASP 360(Nickel-2) | KCX 217(Carbamylated LYS) | mutant C319A | |
| 1fwbC02 |
|
|
|
|
|
HIS 219;ASP 221;HIS 320;ARG 336 | KCX 217(Nickel-1 & -2);HIS 246;HIS 272(Nickel-1);HIS 134;HIS 136;ASP 360(Nickel-2) | KCX 217(Carbamylated LYS) | mutant C319A | |
| 1fwcC02 |
|
|
|
|
|
HIS 219;ASP 221;HIS 320;ARG 336 | KCX 217(Nickel-1 & -2);HIS 246;HIS 272(Nickel-1);HIS 134;HIS 136;ASP 360(Nickel-2) | KCX 217(Carbamylated LYS) | mutant C319A | |
| 1fwdC02 |
|
|
|
|
|
HIS 219;ASP 221;HIS 320;ARG 336 | KCX 217(Nickel-1 & -2);HIS 246;HIS 272(Nickel-1);HIS 134;HIS 136;ASP 360(Nickel-2) | KCX 217(Carbamylated LYS) | mutant C319A | |
| 1fweC02 |
|
|
|
|
|
HIS 219;ASP 221;;ARG 336 | KCX 217(Nickel-1 & -2);HIS 246;HIS 272(Nickel-1);HIS 134;HIS 136;ASP 360(Nickel-2) | KCX 217(Carbamylated LYS) | mutant C319A, invisible 318-331 | |
| 1fwfC02 |
|
|
|
|
|
HIS 219;ASP 221;;ARG 336 | KCX 217(Nickel-1 & -2);HIS 246;HIS 272(Nickel-1);HIS 134;HIS 136;ASP 360(Nickel-2) | KCX 217(Carbamylated LYS) | mutant C319D, invisible 317-331 | |
| 1fwgC02 |
|
|
|
|
|
HIS 219;ASP 221;HIS 320;ARG 336 | KCX 217(Nickel-1 & -2);HIS 246;HIS 272(Nickel-1);HIS 134;HIS 136;ASP 360(Nickel-2) | KCX 217(Carbamylated LYS) | mutant C319S | |
| 1fwhC02 |
|
|
|
|
|
HIS 219;ASP 221;HIS 320;ARG 336 | KCX 217(Nickel-1 & -2);HIS 246;HIS 272(Nickel-1);HIS 134;HIS 136;ASP 360(Nickel-2) | KCX 217(Carbamylated LYS) | mutant C319Y | |
| 1fwiC02 |
|
|
|
|
|
HIS 219;ASP 221;;ARG 336 | KCX 217(Nickel-1 & -2);HIS 246;HIS 272(Nickel-1); ;HIS 136;ASP 360(Nickel-2) | KCX 217(Carbamylated LYS) | mutant H134A, invisible 317-331 | |
| 1fwjC02 |
|
|
|
|
|
HIS 219;ASP 221;HIS 320;ARG 336 | KCX 217(Nickel-1 & -2);HIS 246;HIS 272(Nickel-1);HIS 134;HIS 136;ASP 360(Nickel-2) | KCX 217(Carbamylated LYS) | ||
| 1kauC02 |
|
|
|
|
|
HIS 219;ASP 221;HIS 320;ARG 336 | LYS 217(Nickel-1 & -2);HIS 246;HIS 272(Nickel-1);HIS 134;HIS 136;ASP 360(Nickel-2) | CBX 217(Carbamylated LYS) | ||
| 1kraC02 |
|
|
|
|
|
HIS 219;ASP 221;HIS 320;ARG 336 | LYS 217(Nickel-1 & -2);HIS 246;HIS 272(Nickel-1);HIS 134;HIS 136;ASP 360(Nickel-2) | |||
| 1krbC02 |
|
|
|
|
|
;ASP 221;HIS 320;ARG 336 | KCX 217(Nickel-1 & -2);HIS 246;HIS 272(Nickel-1);HIS 134;HIS 136;ASP 360(Nickel-2) | KCX 217(Carbamylated LYS) | mutant H219A | |
| 1krcC02 |
|
|
|
|
|
HIS 219;ASP 221;;ARG 336 | LYS 217(Nickel-1 & -2);HIS 246;HIS 272(Nickel-1);HIS 134;HIS 136;ASP 360(Nickel-2) | CO2 576(Carbamylated LYS) | mutant H320A | |
| 2kauC02 |
|
|
|
|
|
HIS 219;ASP 221;HIS 320;ARG 336 | KCX 217(Nickel-1 & -2);HIS 246;HIS 272(Nickel-1);HIS 134;HIS 136;ASP 360(Nickel-2) | KCX 217(Carbamylated LYS) | ||
| 3kauC02 |
|
|
|
|
|
HIS 219;ASP 221;HIS 320;ARG 336 | LYS 217(Nickel-1 & -2);HIS 246;HIS 272(Nickel-1);HIS 134;HIS 136;ASP 360(Nickel-2) | |||
| 1ie7C02 |
|
|
|
|
|
HIS 222;ASP 224;HIS 323;ARG 339 | KCX 220(Nickel-1 & -2);HIS 249;HIS 275(Nickel-1);HIS 137;HIS 139;ASP 363(Nickel-2) | KCX 220(Carbamylated LYS) | ||
| 1s3tC02 |
|
|
|
|
|
HIS 222;ASP 224;HIS 323;ARG 339 | KCX 220(Nickel-1 & -2);HIS 249;HIS 275(Nickel-1);HIS 137;HIS 139;ASP 363(Nickel-2) | KCX 220(Carbamylated LYS) | ||
| 1ubpC02 |
|
|
|
|
|
HIS 222;ASP 224;HIS 323;ARG 339 | KCX 220(Nickel-1 & -2);HIS 249;HIS 275(Nickel-1);HIS 137;HIS 139;ASP 363(Nickel-2) | KCX 220(Carbamylated LYS) | ||
| 2ubpC02 |
|
|
|
|
|
HIS 222;ASP 224;HIS 323;ARG 339 | KCX 220(Nickel-1 & -2);HIS 249;HIS 275(Nickel-1);HIS 137;HIS 139;ASP 363(Nickel-2) | KCX 220(Carbamylated LYS) | ||
| 3ubpC02 |
|
|
|
|
|
HIS 222;ASP 224;HIS 323;ARG 339 | KCX 220(Nickel-1 & -2);HIS 249;HIS 275(Nickel-1);HIS 137;HIS 139;ASP 363(Nickel-2) | KCX 220(Carbamylated LYS) | ||
| 4ubpC02 |
|
|
|
|
|
HIS 222;ASP 224;HIS 323;ARG 339 | KCX 220(Nickel-1 & -2);HIS 249;HIS 275(Nickel-1);HIS 137;HIS 139;ASP 363(Nickel-2) | KCX 220(Carbamylated LYS) | ||
| 1a5kB |
|
|
|
|
|
|||||
| 1a5lB |
|
|
|
|
|
|||||
| 1a5mB |
|
|
|
|
|
|||||
| 1a5nB |
|
|
|
|
|
|||||
| 1a5oB |
|
|
|
|
|
|||||
| 1ef2B |
|
|
|
|
|
|||||
| 1ejrB |
|
|
|
|
|
|||||
| 1ejsB |
|
|
|
|
|
|||||
| 1ejtB |
|
|
|
|
|
|||||
| 1ejuB |
|
|
|
|
|
|||||
| 1ejvB |
|
|
|
|
|
|||||
| 1ejwB |
|
|
|
|
|
|||||
| 1ejxB |
|
|
|
|
|
|||||
| 1fwaB |
|
|
|
|
|
|||||
| 1fwbB |
|
|
|
|
|
|||||
| 1fwcB |
|
|
|
|
|
|||||
| 1fwdB |
|
|
|
|
|
|||||
| 1fweB |
|
|
|
|
|
|||||
| 1fwfB |
|
|
|
|
|
|||||
| 1fwgB |
|
|
|
|
|
|||||
| 1fwhB |
|
|
|
|
|
|||||
| 1fwiB |
|
|
|
|
|
|||||
| 1fwjB |
|
|
|
|
|
|||||
| 1kauB |
|
|
|
|
|
|||||
| 1kraB |
|
|
|
|
|
|||||
| 1krbB |
|
|
|
|
|
|||||
| 1krcB |
|
|
|
|
|
|||||
| 2kauB |
|
|
|
|
|
|||||
| 3kauB |
|
|
|
|
|
|||||
| 1ie7B |
|
|
|
|
|
|||||
| 1s3tB |
|
|
|
|
|
|||||
| 1ubpB |
|
|
|
|
|
|||||
| 2ubpB |
|
|
|
|
|
|||||
| 3ubpB |
|
|
|
|
|
|||||
| 4ubpB |
|
|
|
|
|
|||||
| 1a5kA |
|
|
|
|
|
|||||
| 1a5lA |
|
|
|
|
|
|||||
| 1a5mA |
|
|
|
|
|
|||||
| 1a5nA |
|
|
|
|
|
|||||
| 1a5oA |
|
|
|
|
|
|||||
| 1ef2C |
|
|
|
|
|
|||||
| 1ejrA |
|
|
|
|
|
|||||
| 1ejsA |
|
|
|
|
|
|||||
| 1ejtA |
|
|
|
|
|
|||||
| 1ejuA |
|
|
|
|
|
|||||
| 1ejvA |
|
|
|
|
|
|||||
| 1ejwA |
|
|
|
|
|
|||||
| 1ejxA |
|
|
|
|
|
|||||
| 1fwaA |
|
|
|
|
|
|||||
| 1fwbA |
|
|
|
|
|
|||||
| 1fwcA |
|
|
|
|
|
|||||
| 1fwdA |
|
|
|
|
|
|||||
| 1fweA |
|
|
|
|
|
|||||
| 1fwfA |
|
|
|
|
|
|||||
| 1fwgA |
|
|
|
|
|
|||||
| 1fwhA |
|
|
|
|
|
|||||
| 1fwiA |
|
|
|
|
|
|||||
| 1fwjA |
|
|
|
|
|
|||||
| 1kauA |
|
|
|
|
|
|||||
| 1kraA |
|
|
|
|
|
|||||
| 1krbA |
|
|
|
|
|
|||||
| 1krcA |
|
|
|
|
|
|||||
| 2kauA |
|
|
|
|
|
|||||
| 3kauA |
|
|
|
|
|
|||||
| 1ie7A |
|
|
|
|
|
|||||
| 1s3tA |
|
|
|
|
|
|||||
| 1ubpA |
|
|
|
|
|
|||||
| 2ubpA |
|
|
|
|
|
|||||
| 3ubpA |
|
|
|
|
|
|||||
| 4ubpA |
|
|
|
|
|
|||||
| 2fvhA |
|
|
|
|
|
|||||
| 2fvhB |
|
|
|
|
|
|||||
| 2fvhC |
|
|
|
|
|
|||||
| References for Catalytic Mechanism | ||
|---|---|---|
| References | Sections | No. of steps in catalysis |
|
[7]
|
figure, p.996 | |
|
[12]
|
Figure 2, Figure 6, p.333-337 | |
|
[17]
|
Figure 8, p.211-213 | |
|
[19]
|
Figure 7, p.5394-5395 | |
|
[20]
|
FIGURE 1, FIGURE 8, p.8583-8584 | |
|
[22]
|
Figure 2, Figure 3, Figure 4, p.796 | |
|
[23]
|
||
|
[24]
|
Fig.6, p.787 | |
|
[31]
|
Figure 3, Figure 7, Figure 8, Figure 10, p.15335-15337 | |
|
[34]
|
Figure 13, p.6943 | |
|
[35]
|
Figure 4, Figure 11, p.11842 | |
|
[36]
|
Figure 4, Figure 5, Figure 6, Figure 13, Figure 16, Figure 18 | |
|
[37]
|
Figure 1, Figure 2, Figure 3 | |
|
[40]
|
Scheme 2, p.521 | |
| References | |
|---|---|
| [1] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1400317 |
| Journal | J Biol Chem |
| Year | 1992 |
| Volume | 267 |
| Pages | 20024-7 |
| Authors | Martin PR, Hausinger RP |
| Title | Site-directed mutagenesis of the active site cysteine in Klebsiella aerogenes urease. |
| Related PDB | |
| Related UniProtKB | |
| [2] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1404395 |
| Journal | J Mol Biol |
| Year | 1992 |
| Volume | 227 |
| Pages | 934-7 |
| Authors | Jabri E, Lee MH, Hausinger RP, Karplus PA |
| Title | Preliminary crystallographic studies of urease from jack bean and from Klebsiella aerogenes. |
| Related PDB | |
| Related UniProtKB | |
| [3] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8318888 |
| Journal | Protein Sci |
| Year | 1993 |
| Volume | 2 |
| Pages | 1034-41 |
| Authors | Park IS, Hausinger RP |
| Title |
Site-directed mutagenesis of Klebsiella aerogenes urease: identification of histidine residues that appear to function in nickel ligation, |
| Related PDB | |
| Related UniProtKB | |
| [4] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7565414 |
| Journal | Microbiol Rev |
| Year | 1995 |
| Volume | 59 |
| Pages | 451-80 |
| Authors | Mobley HL, Island MD, Hausinger RP |
| Title | Molecular biology of microbial ureases. |
| Related PDB | |
| Related UniProtKB | |
| [5] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8535259 |
| Journal | Protein Sci |
| Year | 1995 |
| Volume | 4 |
| Pages | 2234-6 |
| Authors | Moncrief MB, Hom LG, Jabri E, Karplus PA, Hausinger RP |
| Title | Urease activity in the crystalline state. |
| Related PDB | |
| Related UniProtKB | |
| [6] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS). |
| Medline ID | 95273988 |
| PubMed ID | 7754395 |
| Journal | Science |
| Year | 1995 |
| Volume | 268 |
| Pages | 998-1004 |
| Authors | Jabri E, Carr MB, Hausinger RP, Karplus PA |
| Title | The crystal structure of urease from Klebsiella aerogenes. |
| Related PDB | 1kau 2kau 3kau |
| Related UniProtKB | P18314 P18315 P18316 |
| [7] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7754394 |
| Journal | Science |
| Year | 1995 |
| Volume | 268 |
| Pages | 996-7 |
| Authors | Lippard SJ |
| Title | At last--the crystal structure of urease. |
| Related PDB | |
| Related UniProtKB | |
| [8] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS). |
| Medline ID | 96346054 |
| PubMed ID | 8718850 |
| Journal | Biochemistry |
| Year | 1996 |
| Volume | 35 |
| Pages | 10616-26 |
| Authors | Jabri E, Karplus PA |
| Title | Structures of the Klebsiella aerogenes urease apoenzyme and two active-site mutants. |
| Related PDB | 1kra 1krb 1krc |
| Related UniProtKB | P18314 P18315 P18316 |
| [9] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 8702515 |
| Journal | J Biol Chem |
| Year | 1996 |
| Volume | 271 |
| Pages | 18632-7 |
| Authors | Park IS, Michel LO, Pearson MA, Jabri E, Karplus PA, Wang S, Dong J, Scott RA, Koehler BP, Johnson MK, Hausinger RP |
| Title | Characterization of the mononickel metallocenter in H134A mutant urease. |
| Related PDB | 1fwi |
| Related UniProtKB | |
| [10] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 9201965 |
| Journal | Biochemistry |
| Year | 1997 |
| Volume | 36 |
| Pages | 8164-72 |
| Authors | Pearson MA, Michel LO, Hausinger RP, Karplus PA |
| Title | Structures of Cys319 variants and acetohydroxamate-inhibited Klebsiella aerogenes urease. |
| Related PDB | 1fwa 1fwb 1fwc 1fwd 1fwe 1fwf 1fwg 1fwh 1fwj |
| Related UniProtKB | |
| [11] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9144792 |
| Journal | Proteins |
| Year | 1997 |
| Volume | 28 |
| Pages | 72-82 |
| Authors | Holm L, Sander C |
| Title | An evolutionary treasure: unification of a broad set of amidohydrolases related to urease. |
| Related PDB | |
| Related UniProtKB | |
| [12] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | |
| Journal | Acc Chem Res |
| Year | 1997 |
| Volume | 30 |
| Pages | 330?7 |
| Authors | Karplus PA, Pearson MA, Hausinger RP |
| Title | 70 Years of Crystalline Urease:? What Have We Learned? |
| Related PDB | |
| Related UniProtKB | |
| [13] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS). |
| Medline ID | |
| PubMed ID | 9761912 |
| Journal | Acta Crystallogr D Biol Crystallogr |
| Year | 1998 |
| Volume | 54 |
| Pages | 409-12 |
| Authors | Benini S, Ciurli S, Rypniewski WR, Wilson KS, Mangani S |
| Title | Crystallization and preliminary high-resolution X-ray diffraction analysis of native and beta-mercaptoethanol-inhibited urease from Bacillus pasteurii. |
| Related PDB | |
| Related UniProtKB | P41020 P41021 P41022 |
| [14] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS). |
| Medline ID | 98226663 |
| PubMed ID | 9558361 |
| Journal | Biochemistry |
| Year | 1998 |
| Volume | 37 |
| Pages | 6214-20 |
| Authors | Pearson MA, Schaller RA, Michel LO, Karplus PA, Hausinger RP |
| Title | Chemical rescue of Klebsiella aerogenes urease variants lacking the carbamylated-lysine nickel ligand. |
| Related PDB | 1a5k 1a5l 1a5m 1a5n 1a5o |
| Related UniProtKB | P18314 P18315 P18316 |
| [15] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | |
| Journal | J Biol Inorg Chem |
| Year | 1998 |
| Volume | 3 |
| Pages | 268-73 |
| Authors | Benini S, Rypniewski WR, Wilson KS, Ciurli S, Mangani S |
| Title | The complex of Bacillus pasteurii urease with beta-mercaptoethanol from X-ray data at 1.65-angstrom resolution. |
| Related PDB | 1ubp |
| Related UniProtKB | |
| [16] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS). |
| Medline ID | |
| PubMed ID | 10555581 |
| Journal | J Biol Inorg Chem |
| Year | 1999 |
| Volume | 4 |
| Pages | 468-77 |
| Authors | Yamaguchi K, Cosper NJ, Stalhandske C, Scott RA, Pearson MA, Karplus PA, Hausinger RP |
| Title | Characterization of metal-substituted Klebsiella aerogenes urease. |
| Related PDB | 1ef2 |
| Related UniProtKB | P18314 P18315 P18316 |
| [17] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS). |
| Medline ID | 99148127 |
| PubMed ID | 10368287 |
| Journal | Structure Fold Des |
| Year | 1999 |
| Volume | 7 |
| Pages | 205-16 |
| Authors | Benini S, Rypniewski WR, Wilson KS, Miletti S, Ciurli S, Mangani S |
| Title | A new proposal for urease mechanism based on the crystal structures of the native and inhibited enzyme from Bacillus pasteurii: why urea hydrolysis costs two nickels. |
| Related PDB | 2ubp 3ubp |
| Related UniProtKB | P41020 P41021 P41022 |
| [18] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11996109 |
| Journal | Acta Biochim Pol |
| Year | 2000 |
| Volume | 47 |
| Pages | 1189-95 |
| Authors | Sirko A, Brodzik R |
| Title | Plant ureases: roles and regulation. |
| Related PDB | |
| Related UniProtKB | |
| [19] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10820010 |
| Journal | Biochemistry |
| Year | 2000 |
| Volume | 39 |
| Pages | 5389-96 |
| Authors | Todd MJ, Hausinger RP |
| Title |
Fluoride inhibition of Klebsiella aerogenes urease: mechanistic implications of a pseudo-uncompetitive, |
| Related PDB | |
| Related UniProtKB | |
| [20] | |
| Resource | |
| Comments |
X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS), |
| Medline ID | |
| PubMed ID | 10913264 |
| Journal | Biochemistry |
| Year | 2000 |
| Volume | 39 |
| Pages | 8575-84 |
| Authors | Pearson MA, Park IS, Schaller RA, Michel LO, Karplus PA, Hausinger RP |
| Title | Kinetic and structural characterization of urease active site variants. |
| Related PDB | 1ejr 1ejs 1ejt 1eju 1ejv |
| Related UniProtKB | P18314 P18315 P18316 |
| [21] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 10766443 |
| Journal | J Biol Inorg Chem |
| Year | 2000 |
| Volume | 5 |
| Pages | 110-8 |
| Authors | Benini S, Rypniewski WR, Wilson KS, Miletti S, Ciurli S, Mangani S |
| Title | The complex of Bacillus pasteurii urease with acetohydroxamate anion from X-ray data at 1.55 A resolution. |
| Related PDB | 4ubp |
| Related UniProtKB | |
| [22] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10798524 |
| Journal | J Biomol Struct Dyn |
| Year | 2000 |
| Volume | 17 |
| Pages | 787-97 |
| Authors | Zimmer M |
| Title | Molecular mechanics evaluation of the proposed mechanisms for the degradation of urea by urease. |
| Related PDB | |
| Related UniProtKB | |
| [23] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | |
| Journal | J Am Chem Soc |
| Year | 2000 |
| Volume | 122 |
| Pages | 9172-77 |
| Authors | Barrios AM, Lippard SJ |
| Title | Interaction of Urea with a Hydroxide-Bridged Dinuclear Nickel Center:? An Alternative Model for the Mechanism of Urease. |
| Related PDB | |
| Related UniProtKB | |
| [24] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 11713685 |
| Journal | J Biol Inorg Chem |
| Year | 2001 |
| Volume | 6 |
| Pages | 778-90 |
| Authors | Benini S, Rypniewski WR, Wilson KS, Ciurli S, Mangani S |
| Title | Structure-based rationalization of urease inhibition by phosphate: novel insights into the enzyme mechanism. |
| Related PDB | 1ie7 |
| Related UniProtKB | |
| [25] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11315566 |
| Journal | J Biol Inorg Chem |
| Year | 2001 |
| Volume | 6 |
| Pages | 300-14 |
| Authors | Musiani F, Arnofi E, Casadio R, Ciurli S |
| Title | Structure-based computational study of the catalytic and inhibition mechanisms of urease. |
| Related PDB | |
| Related UniProtKB | |
| [26] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11373609 |
| Journal | Nat Struct Biol |
| Year | 2001 |
| Volume | 8 |
| Pages | 480-2 |
| Authors | Dunn BE, Grutter MG |
| Title | Helicobacter pylori springs another surprise. |
| Related PDB | |
| Related UniProtKB | |
| [27] | |
| Resource | |
| Comments |
X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS), |
| Medline ID | |
| PubMed ID | 11373617 |
| Journal | Nat Struct Biol |
| Year | 2001 |
| Volume | 8 |
| Pages | 505-9 |
| Authors | Ha NC, Oh ST, Sung JY, Cha KA, Lee MH, Oh BH |
| Title | Supramolecular assembly and acid resistance of Helicobacter pylori urease. |
| Related PDB | 1e9y 1e9z |
| Related UniProtKB | P14916 P69996 |
| [28] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11300826 |
| Journal | Inorg Chem |
| Year | 2001 |
| Volume | 40 |
| Pages | 1250-5 |
| Authors | Barrios AM, Lippard SJ |
| Title | Decomposition of alkyl-substituted urea molecules at a hydroxide-bridged dinickel center. |
| Related PDB | |
| Related UniProtKB | |
| [29] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11807281 |
| Journal | Acta Crystallogr D Biol Crystallogr |
| Year | 2002 |
| Volume | 58 |
| Pages | 374-6 |
| Authors | Sheridan L, Wilmot CM, Cromie KD, van der Logt P, Phillips SE |
| Title | Crystallization and preliminary X-ray structure determination of jack bean urease with a bound antibody fragment. |
| Related PDB | |
| Related UniProtKB | |
| [30] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12121941 |
| Journal | Antimicrob Agents Chemother |
| Year | 2002 |
| Volume | 46 |
| Pages | 2613-8 |
| Authors | Mishra H, Parrill AL, Williamson JS |
| Title | Three-dimensional quantitative structure-activity relationship and comparative molecular field analysis of dipeptide hydroxamic acid Helicobacter pylori urease inhibitors. |
| Related PDB | |
| Related UniProtKB | |
| [31] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 14664576 |
| Journal | J Am Chem Soc |
| Year | 2003 |
| Volume | 125 |
| Pages | 15324-37 |
| Authors | Suarez D, Diaz N, Merz KM Jr |
| Title | Ureases: quantum chemical calculations on cluster models. |
| Related PDB | |
| Related UniProtKB | |
| [32] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12913138 |
| Journal | Plant Physiol |
| Year | 2003 |
| Volume | 132 |
| Pages | 1801-10 |
| Authors | Goldraij A, Beamer LJ, Polacco JC |
| Title | Interallelic complementation at the ubiquitous urease coding locus of soybean. |
| Related PDB | |
| Related UniProtKB | |
| [33] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 15038715 |
| Journal | J Am Chem Soc |
| Year | 2004 |
| Volume | 126 |
| Pages | 3714-5 |
| Authors | Benini S, Rypniewski WR, Wilson KS, Mangani S, Ciurli S |
| Title | Molecular details of urease inhibition by boric acid: insights into the catalytic mechanism. |
| Related PDB | 1s3t |
| Related UniProtKB | |
| [34] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 15174863 |
| Journal | J Am Chem Soc |
| Year | 2004 |
| Volume | 126 |
| Pages | 6932-44 |
| Authors | Estiu G, Merz KM Jr |
| Title | The hydrolysis of urea and the proficiency of urease. |
| Related PDB | |
| Related UniProtKB | |
| [35] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 15382918 |
| Journal | J Am Chem Soc |
| Year | 2004 |
| Volume | 126 |
| Pages | 11832-42 |
| Authors | Estiu G, Merz KM Jr |
| Title | Enzymatic catalysis of urea decomposition: elimination or hydrolysis? |
| Related PDB | |
| Related UniProtKB | |
| [36] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 16584179 |
| Journal | Biochemistry |
| Year | 2006 |
| Volume | 45 |
| Pages | 4429-43 |
| Authors | Estiu G, Merz KM Jr |
| Title |
Catalyzed decomposition of urea. |
| Related PDB | |
| Related UniProtKB | |
| [37] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 17676790 |
| Journal | J Phys Chem B |
| Year | 2007 |
| Volume | 111 |
| Pages | 10263-74 |
| Authors | Estiu G, Merz KM Jr |
| Title | Competitive hydrolytic and elimination mechanisms in the urease catalyzed decomposition of urea. |
| Related PDB | |
| Related UniProtKB | |
| [38] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 20471401 |
| Journal | J Mol Biol |
| Year | 2010 |
| Volume | 400 |
| Pages | 274-83 |
| Authors | Balasubramanian A, Ponnuraj K |
| Title | Crystal structure of the first plant urease from jack bean: 83 years of journey from its first crystal to molecular structure. |
| Related PDB | 3la4 |
| Related UniProtKB | |
| [39] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 21788478 |
| Journal | Proc Natl Acad Sci U S A |
| Year | 2011 |
| Volume | 108 |
| Pages | 13095-9 |
| Authors | Carter EL, Tronrud DE, Taber SR, Karplus PA, Hausinger RP |
| Title | Iron-containing urease in a pathogenic bacterium. |
| Related PDB | 3qga 3qgk |
| Related UniProtKB | |
| [40] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 21542631 |
| Journal | Acc Chem Res |
| Year | 2011 |
| Volume | 44 |
| Pages | 520-30 |
| Authors | Zambelli B, Musiani F, Benini S, Ciurli S |
| Title |
Chemistry of Ni2+ in urease: sensing, |
| Related PDB | |
| Related UniProtKB | |
| Comments |
|---|
|
(ii) Hydrolysis by nucleophilic attack of a bridging hydroxide: Carbonyl oxygen of urea is bound to Nickel-1, (iii) Elimination through cyanic acid (see [23], Alghough these reported mechanisms lead to a debate, There are several types of urease enzymes based on the composition of subunits. According to the literature, (i) Hydrolysis by a reverse protonation mechanism: Carbonyl oxygen of urea is bound to Nickel-1, |
| Created | Updated |
|---|---|
| 2005-10-18 | 2012-03-23 |