DB code: M00199
| RLCP classification | 10.22025.100.10765 : Electron transfer | |
|---|---|---|
| 1.15.8230.352 : Hydrolysis | ||
| 10.25130.110.10590 : Electron transfer | ||
| CATH domain | 3.40.50.1980 : Rossmann fold | Catalytic domain |
| 3.40.50.1980 : Rossmann fold | Catalytic domain | |
| 3.40.50.1980 : Rossmann fold | Catalytic domain | |
| 3.40.50.1980 : Rossmann fold | Catalytic domain | |
| 3.40.50.1980 : Rossmann fold | ||
| 3.40.50.1980 : Rossmann fold | ||
| 1.20.89.10 : Nitrogenase Molybdenum-iron Protein, subunit B; domain 4 | ||
| 3.40.50.300 : Rossmann fold | Catalytic domain | |
| E.C. | 1.18.6.1 | |
| CSA | ||
| M-CSA | ||
| MACiE | ||
| CATH domain | Related DB codes (homologues) |
|---|---|
| 1.20.89.10 : Nitrogenase Molybdenum-iron Protein, subunit B; domain 4 | M00114 |
| 3.40.50.1980 : Rossmann fold | M00114 |
| 3.40.50.300 : Rossmann fold | S00527 S00547 S00548 S00550 S00554 S00555 S00671 S00672 S00676 S00680 S00682 S00913 S00914 S00301 S00302 S00303 S00304 S00307 S00308 S00305 S00306 S00309 S00310 S00311 M00114 D00129 D00130 D00540 M00186 |
| Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | Pfam |
|---|---|---|---|
| P00467 |
Nitrogenase molybdenum-iron protein alpha chain
|
EC
1.18.6.1
Nitrogenase component I Dinitrogenase |
PF00148
(Oxidored_nitro)
[Graphical View] |
| P11347 |
Nitrogenase molybdenum-iron protein beta chain
|
EC
1.18.6.1
Nitrogenase component I Dinitrogenase |
PF00148
(Oxidored_nitro)
[Graphical View] |
| P00456 |
Nitrogenase iron protein 1
|
EC
1.18.6.1
Nitrogenase component II Nitrogenase Fe protein 1 Nitrogenase reductase |
PF00142
(Fer4_NifH)
[Graphical View] |
| KEGG enzyme name |
|---|
|
nitrogenase
|
| UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
|---|---|---|---|---|---|
| P00467 | NIFD_CLOPA | 8 reduced ferredoxin + 8 H(+) + N(2) + 16 ATP + 16 H(2)O = 8 oxidized ferredoxin + H(2) + 2 NH(3) + 16 ADP + 16 phosphate. | Tetramer of two alpha and two beta chains. Forms complex with the iron protein (nitrogenase component 2). | Binds 1 8Fe-7S cluster per heterodimer (By similarity). Binds 1 7Fe-Mo-9S-X-homocitryl cluster per subunit. The identity of the X atom is not known, possibly carbon or oxygen (By similarity). | |
| P11347 | NIFK_CLOPA | 8 reduced ferredoxin + 8 H(+) + N(2) + 16 ATP + 16 H(2)O = 8 oxidized ferredoxin + H(2) + 2 NH(3) + 16 ADP + 16 phosphate. | Tetramer of two alpha and two beta chains. Forms complex with the iron protein (nitrogenase component 2). | Binds 1 8Fe-7S cluster per heterodimer. | |
| P00456 | NIFH1_CLOPA | 8 reduced ferredoxin + 8 H(+) + N(2) + 16 ATP + 16 H(2)O = 8 oxidized ferredoxin + H(2) + 2 NH(3) + 16 ADP + 16 phosphate. | Homodimer. | Binds 1 4Fe-4S cluster per dimer. |
| KEGG Pathways | Map code | Pathways | E.C. |
|---|---|---|
| MAP00625 | Tetrachloroethene degradation | |
| MAP00910 | Nitrogen metabolism |
| Compound table | |||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cofactors | Substrates | Products | Intermediates | ||||||||||||||||||
| KEGG-id | L00026 | L00025 | C01251 | L00024 | C00305 | C00138 | C00080 | C00697 | C00002 | C00001 | C00139 | C00014 | C00008 | C00009 | |||||||
| E.C. | |||||||||||||||||||||
| Compound | [8Fe-7S](P cluster) | [7Fe-9S-Mo](FeMoco) | Homocitrate | [4Fe-4S] | Magnesium | Reduced ferredoxin | H+ | N2 | ATP | H2O | Oxidized ferredoxin | NH3 | ADP | Orthophosphate | Transition-state for ATP hydrolysis | Intermediate for Nitrogenation | |||||
| Type | heavy metal,sulfide group | heavy metal,sulfide group | carbohydrate,carboxyl group | heavy metal,sulfide group | divalent metal (Ca2+, Mg2+) | heavy metal,peptide/protein,sulfide group | others | others | amine group,nucleotide | H2O | heavy metal,peptide/protein,sulfide group | amine group,organic ion | amine group,nucleotide | phosphate group/phosphate ion | |||||||
| ChEBI |
48796 48796 |
52222 52222 |
33725 33725 |
18420 18420 |
15378 15378 |
17997 17997 |
15422 15422 |
15377 15377 |
16134 16134 |
16761 16761 |
26078 26078 |
||||||||||
| PubChem |
439459 439459 |
888 888 |
1038 1038 |
947 947 |
5957 5957 |
22247451 962 22247451 962 |
222 222 |
6022 6022 |
1004 22486802 1004 22486802 |
||||||||||||
| 1mioA01 |
|
|
|
|
|
Unbound | Bound:CFM | Bound:HCA | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |||
| 1mioC01 |
|
|
|
|
|
Unbound | Bound:CFM | Bound:HCA | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |||
| 1mioA02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |||
| 1mioC02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |||
| 1mioA03 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |||
| 1mioC03 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |||
| 1mioB01 |
|
|
|
|
|
Analogue:CLP | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |||
| 1mioD01 |
|
|
|
|
|
Analogue:CLP | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |||
| 1mioB02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |||
| 1mioD02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |||
| 1mioB04 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |||
| 1mioD04 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |||
| 1mioB03 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |||
| 1mioD03 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |||
| 1cp2A |
|
|
|
|
|
Unbound | Unbound | Unbound | Bound:SF4 | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |||
| 1cp2B |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |||
| Reference for Active-site residues | ||
|---|---|---|
| resource | references | E.C. |
| Active-site residues | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| PDB | Catalytic residues | Cofactor-binding residues | Modified residues | Main-chain involved in catalysis | Comment | |||||
| 1mioA01 |
|
|
|
|
|
CYS 53 | CYS 53(P cluster);ILE 465;HIS 482(Homocitrate);HIS 482(FeMoco) | |||
| 1mioC01 |
|
|
|
|
|
CYS 53 | CYS 53(P cluster);ILE 465;HIS 482(Homocitrate);HIS 482(FeMoco) | |||
| 1mioA02 |
|
|
|
|
|
GLN 182 | GLN 182(Homocitrate);CYS 79;CYS 145(P cluster) | |||
| 1mioC02 |
|
|
|
|
|
GLN 182 | GLN 182(Homocitrate);CYS 79;CYS 145(P cluster) | |||
| 1mioA03 |
|
|
|
|
|
CYS 262(FeMoco) | ||||
| 1mioC03 |
|
|
|
|
|
CYS 262(FeMoco) | ||||
| 1mioB01 |
|
|
|
|
|
CYS 106;THR 110 | CYS 23;CYS 48;CYS 106;SER 141(P cluster) | |||
| 1mioD01 |
|
|
|
|
|
CYS 106;THR 110 | CYS 23;CYS 48;CYS 106;SER 141(P cluster) | |||
| 1mioB02 |
|
|
|
|
|
|||||
| 1mioD02 |
|
|
|
|
|
|||||
| 1mioB04 |
|
|
|
|
|
|||||
| 1mioD04 |
|
|
|
|
|
|||||
| 1mioB03 |
|
|
|
|
|
|||||
| 1mioD03 |
|
|
|
|
|
|||||
| 1cp2A |
|
|
|
|
|
LYS 9;LYS 14;ASP 38;LYS 40;ASP 126 | CYS 94;CYS 129(4Fe-4S binding);SER 15(Magnesium binding) | GLY 11 | ||
| 1cp2B |
|
|
|
|
|
LYS 9;LYS 14;ASP 38;LYS 40;ASP 126 | CYS 94;CYS 129(4Fe-4S binding);SER 15(Magnesium binding) | GLY 11 | ||
| References for Catalytic Mechanism | ||
|---|---|---|
| References | Sections | No. of steps in catalysis |
|
[5]
|
Fig.2 | |
|
[12]
|
Scheme 1, Scheme 2, Scheme 3 | |
|
[14]
|
p.1680-1681 | |
|
[16]
|
p.1657-1658 | |
|
[17]
|
p.994 | |
|
[18]
|
Fig.9, p.347-349 | |
|
[20]
|
p.7112-7113 | |
|
[24]
|
Fig.2, Fig.3, p.794 | |
|
[33]
|
p.27012-27013 | |
|
[35]
|
p.10722 | |
|
[36]
|
p.13115-13117 | |
|
[40]
|
p.16774-16775 | |
|
[41]
|
p.9431-9432 | |
|
[42]
|
p.15660-15661 | |
|
[46]
|
Scheme 1, p.12981-12982 | |
|
[47]
|
Fig.2, Fig.4 | |
|
[50]
|
p.374-375 | |
|
[52]
|
p.11381-11383 | |
|
[53]
|
p.404-406 | |
|
[64]
|
p.881-886 | |
|
[68]
|
p.14750-14751 | |
|
[71]
|
p.563-564 | |
|
[73]
|
Scheme 1, Scheme 2, Fig.3, Fig.4, p.571-575 | |
|
[74]
|
p.13824 | |
|
[75]
|
p.648-650 | |
|
[78]
|
||
|
[81]
|
Fig.6 | |
|
[86]
|
Fig.7, Fig.8 | |
|
[87]
|
Fig.5, p.1698-1699 | |
|
[89]
|
Scheme 1, Scheme 2, Scheme 3, Scheme 4 | |
|
[91]
|
p.1032-1033 | |
|
[93]
|
p.380-381 | |
|
[94]
|
Fig.3 | |
|
[95]
|
Fig.8, p.1048-1051 | |
| References | |
|---|---|
| [1] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 6246963 |
| Journal | Biochim Biophys Acta |
| Year | 1980 |
| Volume | 623 |
| Pages | 124-38 |
| Authors | Huynh BH, Henzl MT, Christner JA, Zimmermann R, Orme-Johnson WH, Munck E |
| Title |
Nitrogenase XII. |
| Related PDB | |
| Related UniProtKB | |
| [2] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 6938202 |
| Journal | Biochem Biophys Res Commun |
| Year | 1981 |
| Volume | 98 |
| Pages | 43-50 |
| Authors | Meyer J, Zaccai G |
| Title | Neutron small angle scattering of the Mo-Fe protein (nitrogenase) from Clostridium pasteurianum. |
| Related PDB | |
| Related UniProtKB | |
| [3] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 6948813 |
| Journal | J Biol Chem |
| Year | 1982 |
| Volume | 257 |
| Pages | 1221-3 |
| Authors | Yamane T, Weininger MS, Mortenson LE, Rossmann MG |
| Title |
Molecular symmetry of the MoFe protein of nitrogenase. |
| Related PDB | |
| Related UniProtKB | |
| [4] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 6952190 |
| Journal | Proc Natl Acad Sci U S A |
| Year | 1982 |
| Volume | 79 |
| Pages | 378-80 |
| Authors | Weininger MS, Mortenson LE |
| Title | Crystallographic properties of the MoFe proteins of nitrogenase from Clostridium pasteurianum and Azotobacter vinelandii. |
| Related PDB | |
| Related UniProtKB | |
| [5] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 3890886 |
| Journal | Annu Rev Biophys Biophys Chem |
| Year | 1985 |
| Volume | 14 |
| Pages | 419-59 |
| Authors | Orme-Johnson WH |
| Title | Molecular basis of biological nitrogen fixation. |
| Related PDB | |
| Related UniProtKB | |
| [6] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2840948 |
| Journal | Biochemistry |
| Year | 1988 |
| Volume | 27 |
| Pages | 2800-10 |
| Authors | Wang SZ, Chen JS, Johnson JL |
| Title | Distinct structural features of the alpha and beta subunits of nitrogenase molybdenum-iron protein of Clostridium pasteurianum: an analysis of amino acid sequences. |
| Related PDB | |
| Related UniProtKB | |
| [7] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2842451 |
| Journal | J Inorg Biochem |
| Year | 1988 |
| Volume | 33 |
| Pages | 111-20 |
| Authors | Morgan TV, Mortenson LE, McDonald JW, Watt GD |
| Title | Comparison of redox and EPR properties of the molybdenum iron proteins of Clostridium pasteurianum and Azotobacter vinelandii nitrogenases. |
| Related PDB | |
| Related UniProtKB | |
| [8] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2847787 |
| Journal | Biochemistry |
| Year | 1988 |
| Volume | 27 |
| Pages | 6150-6 |
| Authors | Meyer J, Gaillard J, Moulis JM |
| Title | Hydrogen-1 nuclear magnetic resonance of the nitrogenase iron protein (Cp2) from Clostridium pasteurianum. |
| Related PDB | |
| Related UniProtKB | |
| [9] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2554893 |
| Journal | Biochem J |
| Year | 1989 |
| Volume | 262 |
| Pages | 349-52 |
| Authors | George GN, Bare RE, Jin HY, Stiefel EI, Prince RC |
| Title | E.p.r.-spectroscopic studies on the molybdenum-iron site of nitrogenase from Clostridium pasteurianum. |
| Related PDB | |
| Related UniProtKB | |
| [10] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2153269 |
| Journal | Nature |
| Year | 1990 |
| Volume | 343 |
| Pages | 188-90 |
| Authors | Scott DJ, May HD, Newton WE, Brigle KE, Dean DR |
| Title | Role for the nitrogenase MoFe protein alpha-subunit in FeMo-cofactor binding and catalysis. |
| Related PDB | |
| Related UniProtKB | |
| [11] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2159783 |
| Journal | Biochemistry |
| Year | 1990 |
| Volume | 29 |
| Pages | 3077-82 |
| Authors | Morgan TV, McCracken J, Orme-Johnson WH, Mims WB, Mortenson LE, Peisach J |
| Title | Pulsed electron paramagnetic resonance studies of the interaction of Mg-ATP and D2O with the iron protein of nitrogenase. |
| Related PDB | |
| Related UniProtKB | |
| [12] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1555570 |
| Journal | Eur J Biochem |
| Year | 1992 |
| Volume | 205 |
| Pages | 1-15 |
| Authors | Smith BE, Eady RR |
| Title | Metalloclusters of the nitrogenases. |
| Related PDB | |
| Related UniProtKB | |
| [13] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) |
| Medline ID | |
| PubMed ID | |
| Journal | Nature |
| Year | 1992 |
| Volume | 360 |
| Pages | 553-60 |
| Authors | Kim J, Rees DC |
| Title | Crystallographic structure and functional implications of the nitrogenase molybdenum-iron protein from Azotobacter vinelandii. |
| Related PDB | |
| Related UniProtKB | P07328 P07329 |
| [14] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1529354 |
| Journal | Science |
| Year | 1992 |
| Volume | 257 |
| Pages | 1677-82 |
| Authors | Kim J, Rees DC |
| Title | Structural models for the metal centers in the nitrogenase molybdenum-iron protein. |
| Related PDB | |
| Related UniProtKB | |
| [15] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1740419 |
| Journal | J Biol Chem |
| Year | 1992 |
| Volume | 267 |
| Pages | 3667-73 |
| Authors | Wolle D, Kim C, Dean D, Howard JB |
| Title |
Ionic interactions in the nitrogenase complex. |
| Related PDB | |
| Related UniProtKB | |
| [16] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS). |
| Medline ID | 92410322 |
| PubMed ID | 1529353 |
| Journal | Science |
| Year | 1992 |
| Volume | 257 |
| Pages | 1653-9 |
| Authors | Georgiadis MM, Komiya H, Chakrabarti P, Woo D, Kornuc JJ, Rees DC |
| Title | Crystallographic structure of the nitrogenase iron protein from Azotobacter vinelandii. |
| Related PDB | 1nip |
| Related UniProtKB | P00459 |
| [17] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1359643 |
| Journal | Science |
| Year | 1992 |
| Volume | 258 |
| Pages | 992-5 |
| Authors | Wolle D, Dean DR, Howard JB |
| Title | Nucleotide-iron-sulfur cluster signal transduction in the nitrogenase iron-protein: the role of Asp125. |
| Related PDB | |
| Related UniProtKB | |
| [18] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8322617 |
| Journal | Adv Enzymol Relat Areas Mol Biol |
| Year | 1993 |
| Volume | 67 |
| Pages | 299-374 |
| Authors | Mortenson LE, Seefeldt LC, Morgan TV, Bolin JT |
| Title | The role of metal clusters and MgATP in nitrogenase catalysis. |
| Related PDB | |
| Related UniProtKB | |
| [19] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8392330 |
| Journal | Biochem J |
| Year | 1993 |
| Volume | 293 |
| Pages | 101-7 |
| Authors | Pau RN, Eldridge ME, Lowe DJ, Mitchenall LA, Eady RR |
| Title | Molybdenum-independent nitrogenases of Azotobacter vinelandii: a functional species of alternative nitrogenase-3 isolated from a molybdenum-tolerant strain contains an iron-molybdenum cofactor. |
| Related PDB | |
| Related UniProtKB | |
| [20] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS |
| Medline ID | 93344379 |
| PubMed ID | 8393705 |
| Journal | Biochemistry |
| Year | 1993 |
| Volume | 32 |
| Pages | 7104-15 |
| Authors | Kim J, Woo D, Rees DC |
| Title | X-ray crystal structure of the nitrogenase molybdenum-iron protein from Clostridium pasteurianum at 3.0-A resolution. |
| Related PDB | 1mio |
| Related UniProtKB | P00467 P11347 |
| [21] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8389587 |
| Journal | Biochemistry |
| Year | 1993 |
| Volume | 32 |
| Pages | 6058-64 |
| Authors | Oliver ME, Hales BJ |
| Title | Using dysprosium complexes to probe the nitrogenase paramagnetic centers. |
| Related PDB | |
| Related UniProtKB | |
| [22] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8440368 |
| Journal | FEBS Lett |
| Year | 1993 |
| Volume | 318 |
| Pages | 118-24 |
| Authors | Gerloff DL, Jenny TF, Knecht LJ, Gonnet GH, Benner SA |
| Title |
The nitrogenase MoFe protein. |
| Related PDB | |
| Related UniProtKB | |
| [23] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8430077 |
| Journal | Proc Natl Acad Sci U S A |
| Year | 1993 |
| Volume | 90 |
| Pages | 1078-82 |
| Authors | Bolin JT, Ronco AE, Morgan TV, Mortenson LE, Xuong NH |
| Title | The unusual metal clusters of nitrogenase: structural features revealed by x-ray anomalous diffraction studies of the MoFe protein from Clostridium pasteurianum. |
| Related PDB | |
| Related UniProtKB | |
| [24] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8484118 |
| Journal | Science |
| Year | 1993 |
| Volume | 260 |
| Pages | 792-4 |
| Authors | Chan MK, Kim J, Rees DC |
| Title | The nitrogenase FeMo-cofactor and P-cluster pair: 2.2 A resolution structures. |
| Related PDB | |
| Related UniProtKB | |
| [25] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8140948 |
| Journal | Adv Inorg Biochem |
| Year | 1994 |
| Volume | 9 |
| Pages | 219-43 |
| Authors | Clague MJ, Butler A |
| Title | Functional mimics of vanadium enzymes. |
| Related PDB | |
| Related UniProtKB | |
| [26] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7918402 |
| Journal | Biochemistry |
| Year | 1994 |
| Volume | 33 |
| Pages | 11842-9 |
| Authors | Gavini N, Ma L, Watt G, Burgess BK |
| Title | Purification and characterization of a FeMo cofactor-deficient MoFe protein. |
| Related PDB | |
| Related UniProtKB | |
| [27] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8106367 |
| Journal | J Biol Chem |
| Year | 1994 |
| Volume | 269 |
| Pages | 3290-4 |
| Authors | Chen L, Gavini N, Tsuruta H, Eliezer D, Burgess BK, Doniach S, Hodgson KO |
| Title |
MgATP-induced conformational changes in the iron protein from Azotobacter vinelandii, |
| Related PDB | |
| Related UniProtKB | |
| [28] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7961744 |
| Journal | J Biol Chem |
| Year | 1994 |
| Volume | 269 |
| Pages | 28076-83 |
| Authors | Peters JW, Fisher K, Dean DR |
| Title | Identification of a nitrogenase protein-protein interaction site defined by residues 59 through 67 within the Azotobacter vinelandii Fe protein. |
| Related PDB | |
| Related UniProtKB | |
| [29] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8034623 |
| Journal | J Biol Chem |
| Year | 1994 |
| Volume | 269 |
| Pages | 18723-6 |
| Authors | Zheng L, Dean DR |
| Title | Catalytic formation of a nitrogenase iron-sulfur cluster. |
| Related PDB | |
| Related UniProtKB | |
| [30] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7703853 |
| Journal | Protein Sci |
| Year | 1994 |
| Volume | 3 |
| Pages | 2073-81 |
| Authors | Seefeldt LC |
| Title | Docking of nitrogenase iron- and molybdenum-iron proteins for electron transfer and MgATP hydrolysis: the role of arginine 140 and lysine 143 of the Azotobacter vinelandii iron protein. |
| Related PDB | |
| Related UniProtKB | |
| [31] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8561464 |
| Journal | Annu Rev Microbiol |
| Year | 1995 |
| Volume | 49 |
| Pages | 335-66 |
| Authors | Peters JW, Fisher K, Dean DR |
| Title | Nitrogenase structure and function: a biochemical-genetic perspective. |
| Related PDB | |
| Related UniProtKB | |
| [32] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7893691 |
| Journal | Biochemistry |
| Year | 1995 |
| Volume | 34 |
| Pages | 2798-808 |
| Authors | Kim CH, Newton WE, Dean DR |
| Title | Role of the MoFe protein alpha-subunit histidine-195 residue in FeMo-cofactor binding and nitrogenase catalysis. |
| Related PDB | |
| Related UniProtKB | |
| [33] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7592949 |
| Journal | J Biol Chem |
| Year | 1995 |
| Volume | 270 |
| Pages | 27007-13 |
| Authors | Peters JW, Fisher K, Newton WE, Dean DR |
| Title | Involvement of the P cluster in intramolecular electron transfer within the nitrogenase MoFe protein. |
| Related PDB | |
| Related UniProtKB | |
| [34] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7495793 |
| Journal | Biochemistry |
| Year | 1995 |
| Volume | 34 |
| Pages | 15646-53 |
| Authors | Lanzilotta WN, Holz RC, Seefeldt LC |
| Title | Proton NMR investigation of the [4Fe--4S]1+ cluster environment of nitrogenase iron protein from Azotobacter vinelandii: defining nucleotide-induced conformational changes. |
| Related PDB | |
| Related UniProtKB | |
| [35] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7662655 |
| Journal | Biochemistry |
| Year | 1995 |
| Volume | 34 |
| Pages | 10713-23 |
| Authors | Lanzilotta WN, Ryle MJ, Seefeldt LC |
| Title | Nucleotide hydrolysis and protein conformational changes in Azotobacter vinelandii nitrogenase iron protein: defining the function of aspartate 129. |
| Related PDB | |
| Related UniProtKB | |
| [36] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7768906 |
| Journal | J Biol Chem |
| Year | 1995 |
| Volume | 270 |
| Pages | 13112-7 |
| Authors | Ryle MJ, Lanzilotta WN, Mortenson LE, Watt GD, Seefeldt LC |
| Title | Evidence for a central role of lysine 15 of Azotobacter vinelandii nitrogenase iron protein in nucleotide binding and protein conformational changes. |
| Related PDB | |
| Related UniProtKB | |
| [37] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8555217 |
| Journal | Biochemistry |
| Year | 1996 |
| Volume | 35 |
| Pages | 472-8 |
| Authors | Blanchard CZ, Hales BJ |
| Title | Isolation of two forms of the nitrogenase VFe protein from Azotobacter vinelandii. |
| Related PDB | |
| Related UniProtKB | |
| [38] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8679547 |
| Journal | Biochemistry |
| Year | 1996 |
| Volume | 35 |
| Pages | 7188-96 |
| Authors | Lanzilotta WN, Fisher K, Seefeldt LC |
| Title | Evidence for electron transfer from the nitrogenase iron protein to the molybdenum-iron protein without MgATP hydrolysis: characterization of a tight protein-protein complex. |
| Related PDB | |
| Related UniProtKB | |
| [39] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8988014 |
| Journal | Biochemistry |
| Year | 1996 |
| Volume | 35 |
| Pages | 16770-6 |
| Authors | Lanzilotta WN, Seefeldt LC |
| Title | Electron transfer from the nitrogenase iron protein to the [8Fe-(7/8)S] clusters of the molybdenum-iron protein. |
| Related PDB | |
| Related UniProtKB | |
| [40] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8555218 |
| Journal | Biochemistry |
| Year | 1996 |
| Volume | 35 |
| Pages | 479-87 |
| Authors | Tittsworth RC, Hales BJ |
| Title | Oxidative titration of the nitrogenase VFe protein from Azotobacter vinelandii: an example of redox-gated electron flow. |
| Related PDB | |
| Related UniProtKB | |
| [41] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8755721 |
| Journal | Biochemistry |
| Year | 1996 |
| Volume | 35 |
| Pages | 9424-34 |
| Authors | Ryle MJ, Lanzilotta WN, Seefeldt LC |
| Title | Elucidating the mechanism of nucleotide-dependent changes in the redox potential of the [4Fe-4S] cluster in nitrogenase iron protein: the role of phenylalanine 135. |
| Related PDB | |
| Related UniProtKB | |
| [42] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8961928 |
| Journal | Biochemistry |
| Year | 1996 |
| Volume | 35 |
| Pages | 15654-62 |
| Authors | Ryle MJ, Seefeldt LC |
| Title | The [4Fe-4S] cluster domain of the nitrogenase iron protein facilitates conformational changes required for the cooperative binding of two nucleotides. |
| Related PDB | |
| Related UniProtKB | |
| [43] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8664266 |
| Journal | Biochemistry |
| Year | 1996 |
| Volume | 35 |
| Pages | 4766-75 |
| Authors | Ryle MJ, Seefeldt LC |
| Title | Elucidation of a MgATP signal transduction pathway in the nitrogenase iron protein: formation of a conformation resembling the MgATP-bound state by protein engineering. |
| Related PDB | |
| Related UniProtKB | |
| [44] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8576152 |
| Journal | J Biol Chem |
| Year | 1996 |
| Volume | 271 |
| Pages | 1551-7 |
| Authors | Ryle MJ, Lanzilotta WN, Seefeldt LC, Scarrow RC, Jensen GM |
| Title |
Circular dichroism and x-ray spectroscopies of Azotobacter vinelandii nitrogenase iron protein. |
| Related PDB | |
| Related UniProtKB | |
| [45] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9307010 |
| Journal | Biochem J |
| Year | 1997 |
| Volume | 326 |
| Pages | 637-40 |
| Authors | Yousafzai FK, Eady RR |
| Title | Nitrogenase of Klebsiella pneumoniae: kinetics of formation of the transition-state complex and evidence for an altered conformation of MoFe protein lacking a FeMoco centre. |
| Related PDB | |
| Related UniProtKB | |
| [46] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9335558 |
| Journal | Biochemistry |
| Year | 1997 |
| Volume | 36 |
| Pages | 12976-83 |
| Authors | Lanzilotta WN, Seefeldt LC |
| Title | Changes in the midpoint potentials of the nitrogenase metal centers as a result of iron protein-molybdenum-iron protein complex formation. |
| Related PDB | |
| Related UniProtKB | |
| [47] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) |
| Medline ID | 97178802 |
| PubMed ID | 9063865 |
| Journal | Biochemistry |
| Year | 1997 |
| Volume | 36 |
| Pages | 1181-7 |
| Authors | Peters JW, Stowell MH, Soltis SM, Finnegan MG, Johnson MK, Rees DC |
| Title | Redox-dependent structural changes in the nitrogenase P-cluster. |
| Related PDB | 1min 2min 3min |
| Related UniProtKB | P07328 P07329 |
| [48] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9271856 |
| Journal | FEMS Microbiol Lett |
| Year | 1997 |
| Volume | 153 |
| Pages | 303-9 |
| Authors | Zehr JP, Harris D, Dominic B, Salerno J |
| Title | Structural analysis of the Trichodesmium nitrogenase iron protein: implications for aerobic nitrogen fixation activity. |
| Related PDB | |
| Related UniProtKB | |
| [49] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9102457 |
| Journal | J Mol Biol |
| Year | 1997 |
| Volume | 266 |
| Pages | 642-8 |
| Authors | Grossman JG, Hasnain SS, Yousafzai FK, Smith BE, Eady RR |
| Title | The first glimpse of a complex of nitrogenase component proteins by solution X-ray scattering: conformation of the electron transfer transition state complex of Klebsiella pneumoniae nitrogenase. |
| Related PDB | |
| Related UniProtKB | |
| [50] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) |
| Medline ID | 97305952 |
| PubMed ID | 9163420 |
| Journal | Nature |
| Year | 1997 |
| Volume | 387 |
| Pages | 370-6 |
| Authors | Schindelin H, Kisker C, Schlessman JL, Howard JB, Rees DC |
| Title | Structure of ADP x AIF4(-)-stabilized nitrogenase complex and its implications for signal transduction. |
| Related PDB | 1n2c |
| Related UniProtKB | P07328 P07329 |
| [51] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9730834 |
| Journal | Biochemistry |
| Year | 1998 |
| Volume | 37 |
| Pages | 12611-23 |
| Authors | Christiansen J, Goodwin PJ, Lanzilotta WN, Seefeldt LC, Dean DR |
| Title | Catalytic and biophysical properties of a nitrogenase Apo-MoFe protein produced by a nifB-deletion mutant of Azotobacter vinelandii. |
| Related PDB | |
| Related UniProtKB | |
| [52] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9698385 |
| Journal | Biochemistry |
| Year | 1998 |
| Volume | 37 |
| Pages | 11376-84 |
| Authors | Lanzilotta WN, Christiansen J, Dean DR, Seefeldt LC |
| Title | Evidence for coupled electron and proton transfer in the [8Fe-7S] cluster of nitrogenase. |
| Related PDB | |
| Related UniProtKB | |
| [53] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9425061 |
| Journal | Biochemistry |
| Year | 1998 |
| Volume | 37 |
| Pages | 399-407 |
| Authors | Lanzilotta WN, Parker VD, Seefeldt LC |
| Title | Electron transfer in nitrogenase analyzed by Marcus theory: evidence for gating by MgATP. |
| Related PDB | |
| Related UniProtKB | |
| [54] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9710250 |
| Journal | FEBS Lett |
| Year | 1998 |
| Volume | 432 |
| Pages | 55-8 |
| Authors | Spee JH, Arendsen AF, Wassink H, Marritt SJ, Hagen WR, Haaker H |
| Title | Redox properties and electron paramagnetic resonance spectroscopy of the transition state complex of Azotobacter vinelandii nitrogenase. |
| Related PDB | |
| Related UniProtKB | |
| [55] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9756863 |
| Journal | J Biol Chem |
| Year | 1998 |
| Volume | 273 |
| Pages | 26330-7 |
| Authors | Angove HC, Yoo SJ, Munck E, Burgess BK |
| Title |
An all-ferrous state of the Fe protein of nitrogenase. |
| Related PDB | |
| Related UniProtKB | |
| [56] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9792679 |
| Journal | J Biol Chem |
| Year | 1998 |
| Volume | 273 |
| Pages | 29678-85 |
| Authors | Bursey EH, Burgess BK |
| Title | The role of methionine 156 in cross-subunit nucleotide interactions in the iron protein of nitrogenase. |
| Related PDB | |
| Related UniProtKB | |
| [57] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9642255 |
| Journal | J Biol Chem |
| Year | 1998 |
| Volume | 273 |
| Pages | 16927-34 |
| Authors | Bursey EH, Burgess BK |
| Title | Characterization of a variant iron protein of nitrogenase that is impaired in its ability to adopt the MgATP-induced conformational change. |
| Related PDB | |
| Related UniProtKB | |
| [58] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9677296 |
| Journal | J Mol Biol |
| Year | 1998 |
| Volume | 280 |
| Pages | 669-85 |
| Authors | Schlessman JL, Woo D, Joshua-Tor L, Howard JB, Rees DC |
| Title | Conformational variability in structures of the nitrogenase iron proteins from Azotobacter vinelandii and Clostridium pasteurianum. |
| Related PDB | |
| Related UniProtKB | |
| [59] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9860849 |
| Journal | Biochemistry |
| Year | 1998 |
| Volume | 37 |
| Pages | 17345-54 |
| Authors | Duyvis MG, Wassink H, Haaker H |
| Title | Nitrogenase of Azotobacter vinelandii: kinetic analysis of the Fe protein redox cycle. |
| Related PDB | |
| Related UniProtKB | |
| [60] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10215587 |
| Journal | Biochem J |
| Year | 1999 |
| Volume | 339 |
| Pages | 511-5 |
| Authors | Yousafzai FK, Eady RR |
| Title | MgATP-independent hydrogen evolution catalysed by nitrogenase: an explanation for the missing electron(s) in the MgADP-AlF4 transition-state complex. |
| Related PDB | |
| Related UniProtKB | |
| [61] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10231529 |
| Journal | Biochemistry |
| Year | 1999 |
| Volume | 38 |
| Pages | 5779-85 |
| Authors | Chan JM, Christiansen J, Dean DR, Seefeldt LC |
| Title | Spectroscopic evidence for changes in the redox state of the nitrogenase P-cluster during turnover. |
| Related PDB | |
| Related UniProtKB | |
| [62] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10364195 |
| Journal | J Biol Chem |
| Year | 1999 |
| Volume | 274 |
| Pages | 17593-8 |
| Authors | Chan JM, Ryle MJ, Seefeldt LC |
| Title | Evidence that MgATP accelerates primary electron transfer in a Clostridium pasteurianum Fe protein-Azotobacter vinelandii MoFe protein nitrogenase tight complex. |
| Related PDB | |
| Related UniProtKB | |
| [63] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10391920 |
| Journal | J Biol Chem |
| Year | 1999 |
| Volume | 274 |
| Pages | 19778-84 |
| Authors | Rangaraj P, Ryle MJ, Lanzilotta WN, Ludden PW, Shah VK |
| Title |
In vitro biosynthesis of iron-molybdenum cofactor and maturation of the nif-encoded apodinitrogenase. |
| Related PDB | |
| Related UniProtKB | |
| [64] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 10525412 |
| Journal | J Mol Biol |
| Year | 1999 |
| Volume | 292 |
| Pages | 871-91 |
| Authors | Mayer SM, Lawson DM, Gormal CA, Roe SM, Smith BE |
| Title | New insights into structure-function relationships in nitrogenase: A 1.6 A resolution X-ray crystallographic study of Klebsiella pneumoniae MoFe-protein. |
| Related PDB | 1qgu 1qh1 1qh8 |
| Related UniProtKB | |
| [65] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10852721 |
| Journal | Biochemistry |
| Year | 2000 |
| Volume | 39 |
| Pages | 7221-8 |
| Authors | Chan JM, Wu W, Dean DR, Seefeldt LC |
| Title | Construction and characterization of a heterodimeric iron protein: defining roles for adenosine triphosphate in nitrogenase catalysis. |
| Related PDB | |
| Related UniProtKB | |
| [66] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10985789 |
| Journal | Biochemistry |
| Year | 2000 |
| Volume | 39 |
| Pages | 11434-40 |
| Authors | Clarke TA, Maritano S, Eady RR |
| Title | Formation of a tight 1:1 complex of Clostridium pasteurianum Fe protein-Azotobacter vinelandii MoFe protein: evidence for long-range interactions between the Fe protein binding sites during catalytic hydrogen evolution. |
| Related PDB | |
| Related UniProtKB | |
| [67] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11087939 |
| Journal | Biochim Biophys Acta |
| Year | 2000 |
| Volume | 1543 |
| Pages | 36-46 |
| Authors | Johnson JL, Nyborg AC, Wilson PE, Tolley AM, Nordmeyer FR, Watt GD |
| Title | Mechanistic interpretation of the dilution effect for Azotobacter vinelandii and Clostridium pasteurianum nitrogenase catalysis. |
| Related PDB | |
| Related UniProtKB | |
| [68] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS). |
| Medline ID | 20552920 |
| PubMed ID | 11101289 |
| Journal | Biochemistry |
| Year | 2000 |
| Volume | 39 |
| Pages | 14745-52 |
| Authors | Jang SB, Seefeldt LC, Peters JW |
| Title | Insights into nucleotide signal transduction in nitrogenase: structure of an iron protein with MgADP bound. |
| Related PDB | 1fp6 |
| Related UniProtKB | P00459 |
| [69] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10651628 |
| Journal | Biochemistry |
| Year | 2000 |
| Volume | 39 |
| Pages | 641-8 |
| Authors | Jang SB, Seefeldt LC, Peters JW |
| Title | Modulating the midpoint potential of the [4Fe-4S] cluster of the nitrogenase Fe protein. |
| Related PDB | |
| Related UniProtKB | |
| [70] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11087938 |
| Journal | Biochim Biophys Acta |
| Year | 2000 |
| Volume | 1543 |
| Pages | 24-35 |
| Authors | Johnson JL, Nyborg AC, Wilson PE, Tolley AM, Nordmeyer FR, Watt GD |
| Title | Analysis of steady state Fe and MoFe protein interactions during nitrogenase catalysis. |
| Related PDB | |
| Related UniProtKB | |
| [71] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11006545 |
| Journal | Curr Opin Chem Biol |
| Year | 2000 |
| Volume | 4 |
| Pages | 559-66 |
| Authors | Rees DC, Howard JB |
| Title | Nitrogenase: standing at the crossroads. |
| Related PDB | |
| Related UniProtKB | |
| [72] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10837496 |
| Journal | J Biol Chem |
| Year | 2000 |
| Volume | 275 |
| Pages | 17631-8 |
| Authors | Ribbe MW, Bursey EH, Burgess BK |
| Title | Identification of an Fe protein residue (Glu146) of Azotobacter vinelandii nitrogenase that is specifically involved in FeMo cofactor insertion. |
| Related PDB | |
| Related UniProtKB | |
| [73] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11311117 |
| Journal | Biochem J |
| Year | 2001 |
| Volume | 355 |
| Pages | 569-76 |
| Authors | Durrant MC |
| Title | Controlled protonation of iron-molybdenum cofactor by nitrogenase: a structural and theoretical analysis. |
| Related PDB | |
| Related UniProtKB | |
| [74] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11705370 |
| Journal | Biochemistry |
| Year | 2001 |
| Volume | 40 |
| Pages | 13816-25 |
| Authors | Benton PM, Mayer SM, Shao J, Hoffman BM, Dean DR, Seefeldt LC |
| Title | Interaction of acetylene and cyanide with the resting state of nitrogenase alpha-96-substituted MoFe proteins. |
| Related PDB | |
| Related UniProtKB | |
| [75] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) |
| Medline ID | 21093028 |
| PubMed ID | 11170380 |
| Journal | Biochemistry |
| Year | 2001 |
| Volume | 40 |
| Pages | 641-50 |
| Authors | Chiu H, Peters JW, Lanzilotta WN, Ryle MJ, Seefeldt LC, Howard JB, Rees DC |
| Title | MgATP-Bound and nucleotide-free structures of a nitrogenase protein complex between the Leu 127 Delta-Fe-protein and the MoFe-protein. |
| Related PDB | 1g20 1g21 |
| Related UniProtKB | P07328 P07329 |
| [76] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11258953 |
| Journal | Biochemistry |
| Year | 2001 |
| Volume | 40 |
| Pages | 3333-9 |
| Authors | Fisher K, Newton WE, Lowe DJ |
| Title | Electron paramagnetic resonance analysis of different Azotobacter vinelandii nitrogenase MoFe-protein conformations generated during enzyme turnover: evidence for S = 3/2 spin states from reduced MoFe-protein intermediates. |
| Related PDB | |
| Related UniProtKB | |
| [77] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 11327812 |
| Journal | Biochemistry |
| Year | 2001 |
| Volume | 40 |
| Pages | 1540-9 |
| Authors | Sorlie M, Christiansen J, Lemon BJ, Peters JW, Dean DR, Hales BJ |
| Title | Mechanistic features and structure of the nitrogenase alpha-Gln195 MoFe protein. |
| Related PDB | 1fp4 |
| Related UniProtKB | |
| [78] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11734043 |
| Journal | J Am Chem Soc |
| Year | 2001 |
| Volume | 123 |
| Pages | 12392-410 |
| Authors | Lovell T, Li J, Liu T, Case DA, Noodleman L |
| Title |
FeMo cofactor of nitrogenase: a density functional study of states M(N), |
| Related PDB | |
| Related UniProtKB | |
| [79] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS). |
| Medline ID | 21093029 |
| PubMed ID | 11170381 |
| Journal | Biochemistry |
| Year | 2001 |
| Volume | 40 |
| Pages | 651-6 |
| Authors | Strop P, Takahara PM, Chiu H, Angove HC, Burgess BK, Rees DC |
| Title | Crystal structure of the all-ferrous [4Fe-4S]0 form of the nitrogenase iron protein from Azotobacter vinelandii. |
| Related PDB | 1g1m 1g5p |
| Related UniProtKB | P00459 |
| [80] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12045096 |
| Journal | Annu Rev Biochem |
| Year | 2002 |
| Volume | 71 |
| Pages | 221-46 |
| Authors | Rees DC |
| Title | Great metalloclusters in enzymology. |
| Related PDB | |
| Related UniProtKB | |
| [81] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 12501184 |
| Journal | Biochemistry |
| Year | 2002 |
| Volume | 41 |
| Pages | 15557-65 |
| Authors | Schmid B, Einsle O, Chiu HJ, Willing A, Yoshida M, Howard JB, Rees DC |
| Title | Biochemical and structural characterization of the cross-linked complex of nitrogenase: comparison to the ADP-AlF4(-)-stabilized structure. |
| Related PDB | 1m1y 1m34 |
| Related UniProtKB | |
| [82] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12401079 |
| Journal | Inorg Chem |
| Year | 2002 |
| Volume | 41 |
| Pages | 5744-53 |
| Authors | Lovell T, Torres RA, Han WG, Liu T, Case DA, Noodleman L |
| Title |
Metal substitution in the active site of nitrogenase MFe(7)S(9) (M = Mo(4+), |
| Related PDB | |
| Related UniProtKB | |
| [83] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11782173 |
| Journal | J Am Chem Soc |
| Year | 2002 |
| Volume | 124 |
| Pages | 216-24 |
| Authors | Coucouvanis D, Han J, Moon N |
| Title |
Synthesis and characterization of sulfur-voided cubanes. |
| Related PDB | |
| Related UniProtKB | |
| [84] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 12133839 |
| Journal | J Biol Chem |
| Year | 2002 |
| Volume | 277 |
| Pages | 35263-6 |
| Authors | Mayer SM, Gormal CA, Smith BE, Lawson DM |
| Title | Crystallographic analysis of the MoFe protein of nitrogenase from a nifV mutant of Klebsiella pneumoniae identifies citrate as a ligand to the molybdenum of iron molybdenum cofactor (FeMoco). |
| Related PDB | 1h1l |
| Related UniProtKB | |
| [85] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12176981 |
| Journal | J Biol Chem |
| Year | 2002 |
| Volume | 277 |
| Pages | 40106-11 |
| Authors | Rangaraj P, Ludden PW |
| Title |
Accumulation of 99Mo-containing iron-molybdenum cofactor precursors of nitrogenase on NifNE, |
| Related PDB | |
| Related UniProtKB | |
| [86] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11913144 |
| Journal | Met Ions Biol Syst |
| Year | 2002 |
| Volume | 39 |
| Pages | 75-119 |
| Authors | Lawson DM, Smith BE |
| Title | Molybdenum nitrogenases: a crystallographic and mechanistic view. |
| Related PDB | |
| Related UniProtKB | |
| [87] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (1.16 ANGSTROMS) |
| Medline ID | 22204524 |
| PubMed ID | 12215645 |
| Journal | Science |
| Year | 2002 |
| Volume | 297 |
| Pages | 1696-700 |
| Authors | Einsle O, Tezcan FA, Andrade SL, Schmid B, Yoshida M, Howard JB, Rees DC |
| Title | Nitrogenase MoFe-protein at 1.16 A resolution: a central ligand in the FeMo-cofactor. |
| Related PDB | 1m1n |
| Related UniProtKB | P07328 P07329 |
| [88] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) |
| Medline ID | 21948294 |
| PubMed ID | 11951047 |
| Journal | Science |
| Year | 2002 |
| Volume | 296 |
| Pages | 352-6 |
| Authors | Schmid B, Ribbe MW, Einsle O, Yoshida M, Thomas LM, Dean DR, Rees DC, Burgess BK |
| Title | Structure of a cofactor-deficient nitrogenase MoFe protein. |
| Related PDB | 1l5h |
| Related UniProtKB | P07328 P07329 |
| [89] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12506366 |
| Journal | Chemistry |
| Year | 2003 |
| Volume | 9 |
| Pages | 76-87 |
| Authors | Pickett CJ, Vincent KA, Ibrahim SK, Gormal CA, Smith BE, Best SP |
| Title | Electron-transfer chemistry of the iron-molybdenum cofactor of nitrogenase: delocalized and localized reduced states of FeMoco which allow binding of carbon monoxide to iron and molybdenum. |
| Related PDB | |
| Related UniProtKB | |
| [90] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 15102840 |
| Journal | J Biol Chem |
| Year | 2004 |
| Volume | 279 |
| Pages | 28276-82 |
| Authors | Corbett MC, Hu Y, Naderi F, Ribbe MW, Hedman B, Hodgson KO |
| Title | Comparison of iron-molybdenum cofactor-deficient nitrogenase MoFe proteins by X-ray absorption spectroscopy: implications for P-cluster biosynthesis. |
| Related PDB | |
| Related UniProtKB | |
| [91] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 15549494 |
| Journal | J Biol Inorg Chem |
| Year | 2004 |
| Volume | 9 |
| Pages | 1028-33 |
| Authors | Jang SB, Jeong MS, Seefeldt LC, Peters JW |
| Title | Structural and biochemical implications of single amino acid substitutions in the nucleotide-dependent switch regions of the nitrogenase Fe protein from Azotobacter vinelandii. |
| Related PDB | 1xd8 1xd9 1xdb |
| Related UniProtKB | |
| [92] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 14967020 |
| Journal | Biochemistry |
| Year | 2004 |
| Volume | 43 |
| Pages | 1787-97 |
| Authors | Sen S, Igarashi R, Smith A, Johnson MK, Seefeldt LC, Peters JW |
| Title | A conformational mimic of the MgATP-bound "on state" of the nitrogenase iron protein. |
| Related PDB | 1rw4 |
| Related UniProtKB | |
| [93] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 15650336 |
| Journal | Mol Cells |
| Year | 2004 |
| Volume | 18 |
| Pages | 374-82 |
| Authors | Jeong MS, Jang SB |
| Title | Structural basis for the changes in redox potential in the nitrogenase Phe135Trp Fe protein with MgADP Bound. |
| Related PDB | 1xcp |
| Related UniProtKB | |
| [94] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 16123301 |
| Journal | Science |
| Year | 2005 |
| Volume | 309 |
| Pages | 1377-80 |
| Authors | Tezcan FA, Kaiser JT, Mustafi D, Walton MY, Howard JB, Rees DC |
| Title | Nitrogenase complexes: multiple docking sites for a nucleotide switch protein. |
| Related PDB | 2afh 2afi 2afk |
| Related UniProtKB | |
| [95] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 16616373 |
| Journal | J Inorg Biochem |
| Year | 2006 |
| Volume | 100 |
| Pages | 1041-52 |
| Authors | Sen S, Krishnakumar A, McClead J, Johnson MK, Seefeldt LC, Szilagyi RK, Peters JW |
| Title | Insights into the role of nucleotide-dependent conformational change in nitrogenase catalysis: Structural characterization of the nitrogenase Fe protein Leu127 deletion variant with bound MgATP. |
| Related PDB | 2c8v |
| Related UniProtKB | |
| Comments |
|---|
|
This enzyme is homologous to that from Klebsiella pneumoniae and Azotobacter vinelandii (M00114 in EzCatDB).
This enzyme, The Fe protein component is a homodimer, The MoFe protein is a heterotetramer, This enzyme catalyzes the following reactions: (A) Electron transfer from FMN of flavodoxin or [2Fe-2S] of ferredoxin to the [4Fe-4S] cluster of Fe protein: (X) Binding of MgATP molecules to the Fe proteins induces the conformational change, (B) Electron transfer from the [4Fe-4S] cluster to the P-cluster([8Fe-7S]): (C) Hydrolysis of two ATP molecules to produce two ADP molecules, (D) Electron transfer from the P-cluster([8Fe-7S]) to the FeMoco centre: (E) Hydrogenation (H2 production from 2 H+ ions) at the FeMoco centre: (F) Nitorogenation (NH3 production from N2 and H+ ions) at the FeMoco centre: ### This enzyme catalyzes N2 reduction: N2 + 8 H+ + 8 e- => 2 NH3 + H2 (1) 2 H+ + 2 e- => H2 (2) N2 + 6 H+ + 6 e- => 2 NH3 Thus, ### (A) Electron transfer from FMN of flavodoxin or [2Fe-2S] of ferredoxin to the [4Fe-4S] cluster of Fe protein(see [93], (B) Electron transfer from the [4Fe-4S] cluster to the P-cluster([8Fe-7S]): (B1) Indirect transfer from the [4Fe-4S] cluster to the P-cluster through Cys94 (bound to [4Fe-4S]), (C) Hydrolysis of two ATP molecules to produce two ADP molecules on the Fe protein (see [16], (C1) The negative charge of gamma-phsohate group is stabilized by the magnesium ion bound to Ser15, (C2) Either Asp38 or Asp126 from the adjacent subunit acts as a general base to deprotonate and activate the water molecule. (C3) The activated water molecule makes a nucleophilic attack on the phosphorus atom of the gamma-phosphate. (C4) The transition-state must be stabilized by the above stabilizer residues and cofactor. (C5) The reaction completes. (D) Electron transfer from the P-cluster([8Fe-7S]) to the FeMoco centre (see [20], (D1) Indirect transfer from the the P-cluster to the FeMoco centre through Cys53 (bound to P-cluster), (E) Hydrogenation (H2 production from 2 H+ ions) at the FeMoco centre (see [20], (F) Nitorogenation (NH3 production from N2 and H+ ions) at the FeMoco centre (see [24], |
| Created | Updated |
|---|---|
| 2005-04-25 | 2009-02-26 |