DB code: S00672
| RLCP classification | 3.103.69910.364 : Transfer | |
|---|---|---|
| CATH domain | 3.40.50.300 : Rossmann fold | Catalytic domain |
| E.C. | 2.7.1.145 | |
| CSA | ||
| M-CSA | ||
| MACiE | ||
| CATH domain | Related DB codes (homologues) |
|---|---|
| 3.40.50.300 : Rossmann fold | S00527 S00547 S00548 S00550 S00554 S00555 S00671 S00676 S00680 S00682 S00913 S00914 S00301 S00302 S00303 S00304 S00307 S00308 S00305 S00306 S00309 S00310 S00311 M00114 M00199 D00129 D00130 D00540 M00186 |
| Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | RefSeq | Pfam |
|---|---|---|---|---|
| Q9XZT6 |
Deoxynucleoside kinase
|
EC
2.7.1.145
Deoxyribonucleoside kinase Dm-dNK Multispecific deoxynucleoside kinase |
NP_001262722.1
(Protein)
NM_001275793.1 (DNA/RNA sequence) NP_524399.1 (Protein) NM_079675.3 (DNA/RNA sequence) |
PF01712
(dNK)
[Graphical View] |
| KEGG enzyme name |
|---|
|
Deoxynucleoside kinase
Multispecific deoxynucleoside kinase Ms-dNK Multisubstrate deoxyribonucleoside kinase Multifunctional deoxynucleoside kinase D. melanogaster deoxynucleoside kinase Dm-dNK |
| UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
|---|---|---|---|---|---|
| Q9XZT6 | DNK_DROME | ATP + 2'-deoxynucleoside = ADP + 2'-deoxynucleoside 5'-phosphate. | Monomer. |
| KEGG Pathways | Map code | Pathways | E.C. |
|---|
| Compound table | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Cofactors | Substrates | Products | Intermediates | ||||||||
| KEGG-id | C00305 | C00002 | C02269 | C00008 | C00676 | ||||||
| E.C. | |||||||||||
| Compound | Magnesium | ATP | 2'-deoxynucleoside | ADP | 2'-deoxynucleoside 5'-phosphate | ||||||
| Type | divalent metal (Ca2+, Mg2+) | amine group,nucleotide | nucleoside | amine group,nucleotide | nucleotide | ||||||
| ChEBI |
18420 18420 |
15422 15422 |
16761 16761 |
||||||||
| PubChem |
888 888 |
5957 5957 |
6022 6022 |
||||||||
| 1j90A00 |
|
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Unbound | Unbound | Bound:DCZ | Unbound | Unbound | |
| 1j90B00 |
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Unbound | Unbound | Bound:DCZ | Unbound | Unbound | |
| 1oe0A00 |
|
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Bound:_MG | Unbound | Unbound | Unbound | Analogue:TTP | |
| 1oe0B00 |
|
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Bound:_MG | Unbound | Unbound | Unbound | Analogue:TTP | |
| 1oe0C00 |
|
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Bound:_MG | Unbound | Unbound | Unbound | Analogue:TTP | |
| 1oe0D00 |
|
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|
Bound:_MG | Unbound | Unbound | Unbound | Analogue:TTP | |
| 1ot3A00 |
|
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Unbound | Unbound | Bound:THM | Unbound | Unbound | |
| 1ot3B00 |
|
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Unbound | Unbound | Bound:THM | Unbound | Unbound | |
| 1ot3C00 |
|
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Unbound | Unbound | Bound:THM | Unbound | Unbound | |
| 1ot3D00 |
|
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Unbound | Unbound | Bound:THM | Unbound | Unbound | |
| 1ot3E00 |
|
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Unbound | Unbound | Bound:THM | Unbound | Unbound | |
| 1ot3F00 |
|
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Unbound | Unbound | Bound:THM | Unbound | Unbound | |
| 1ot3G00 |
|
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|
Unbound | Unbound | Bound:THM | Unbound | Unbound | |
| 1ot3H00 |
|
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Unbound | Unbound | Bound:THM | Unbound | Unbound | |
| 1zm7A00 |
|
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Unbound | Unbound | Unbound | Unbound | Analogue:TTP | |
| 1zm7B00 |
|
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Unbound | Unbound | Unbound | Unbound | Analogue:TTP | |
| 1zm7C00 |
|
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|
|
Unbound | Unbound | Unbound | Unbound | Analogue:TTP | |
| 1zm7D00 |
|
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|
|
Bound:_MG | Unbound | Unbound | Unbound | Analogue:TTP | |
| 1zmxA00 |
|
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Unbound | Unbound | Bound:THM | Unbound | Unbound | |
| 1zmxB00 |
|
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|
Unbound | Unbound | Bound:THM | Unbound | Unbound | |
| 1zmxC00 |
|
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|
|
|
Unbound | Unbound | Bound:THM | Unbound | Unbound | |
| 1zmxD00 |
|
|
|
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|
Unbound | Unbound | Bound:THM | Unbound | Unbound | |
| 1zmxE00 |
|
|
|
|
|
Unbound | Unbound | Bound:THM | Unbound | Unbound | |
| 1zmxF00 |
|
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|
|
|
Unbound | Unbound | Bound:THM | Unbound | Unbound | |
| 1zmxG00 |
|
|
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|
Unbound | Unbound | Bound:THM | Unbound | Unbound | |
| 1zmxH00 |
|
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|
Unbound | Unbound | Bound:THM | Unbound | Unbound | |
| 2jcsA00 |
|
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|
Bound:_MG | Unbound | Unbound | Unbound | Analogue:TTP | |
| 2jcsB00 |
|
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Bound:_MG | Unbound | Unbound | Unbound | Analogue:TTP | |
| 2jj8A00 |
|
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Unbound | Unbound | Analogue:AAZ | Unbound | Unbound | |
| 2jj8B00 |
|
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Unbound | Unbound | Analogue:AAZ | Unbound | Unbound | |
| 2jj8C00 |
|
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Unbound | Unbound | Analogue:AAZ | Unbound | Unbound | |
| 2jj8D00 |
|
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Unbound | Unbound | Analogue:AAZ | Unbound | Unbound | |
| 2vp0A00 |
|
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|
Bound:_MG | Unbound | Unbound | Unbound | Analogue:TTP | |
| 2vp0B00 |
|
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|
Bound:_MG | Unbound | Unbound | Unbound | Analogue:TTP | |
| 2vp2A00 |
|
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Unbound | Unbound | Unbound | Unbound | Analogue:DGT | |
| 2vp2B00 |
|
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Unbound | Unbound | Unbound | Unbound | Analogue:DGT | |
| 2vp4A00 |
|
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Unbound | Unbound | Unbound | Unbound | Analogue:DCP | |
| 2vp4B00 |
|
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Unbound | Unbound | Unbound | Unbound | Analogue:DCP | |
| 2vp4C00 |
|
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Unbound | Unbound | Unbound | Unbound | Analogue:DCP | |
| 2vp4D00 |
|
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Unbound | Unbound | Unbound | Unbound | Analogue:DCP | |
| 2vp5A00 |
|
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Unbound | Unbound | Bound:DCZ | Unbound | Unbound | |
| 2vp5B00 |
|
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Unbound | Unbound | Bound:DCZ | Unbound | Unbound | |
| 2vp6A00 |
|
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Unbound | Unbound | Analogue:5FU | Unbound | Unbound | |
| 2vp6B00 |
|
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Unbound | Unbound | Analogue:5FU | Unbound | Unbound | |
| 2vp6C00 |
|
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Unbound | Unbound | Analogue:5FU | Unbound | Unbound | |
| 2vp6D00 |
|
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Unbound | Unbound | Analogue:5FU | Unbound | Unbound | |
| 2vp6E00 |
|
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Unbound | Unbound | Analogue:5FU | Unbound | Unbound | |
| 2vp6F00 |
|
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Unbound | Unbound | Analogue:5FU | Unbound | Unbound | |
| 2vp6G00 |
|
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Unbound | Unbound | Analogue:5FU | Unbound | Unbound | |
| 2vp6H00 |
|
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Unbound | Unbound | Analogue:5FU | Unbound | Unbound | |
| 2vp9A00 |
|
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Unbound | Unbound | Analogue:DOC | Unbound | Unbound | |
| 2vp9B00 |
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Unbound | Unbound | Analogue:DOC | Unbound | Unbound | |
| 2vp9C00 |
|
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Unbound | Unbound | Analogue:DOC | Unbound | Unbound | |
| 2vp9D00 |
|
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Unbound | Unbound | Analogue:DOC | Unbound | Unbound | |
| 2vp9E00 |
|
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Unbound | Unbound | Analogue:DOC | Unbound | Unbound | |
| 2vp9F00 |
|
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Unbound | Unbound | Analogue:DOC | Unbound | Unbound | |
| 2vp9G00 |
|
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Unbound | Unbound | Analogue:DOC | Unbound | Unbound | |
| 2vp9H00 |
|
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Unbound | Unbound | Analogue:DOC | Unbound | Unbound | |
| 2vppA00 |
|
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Unbound | Unbound | Analogue:GEO | Unbound | Unbound | |
| 2vppB00 |
|
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Unbound | Unbound | Analogue:GEO | Unbound | Unbound | |
| 2vqsA00 |
|
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Unbound | Unbound | Analogue:BVD | Unbound | Unbound | |
| 2vqsB00 |
|
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Unbound | Unbound | Analogue:BVD | Unbound | Unbound | |
| 2vqsC00 |
|
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Unbound | Unbound | Analogue:BVD | Unbound | Unbound | |
| 2vqsD00 |
|
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Unbound | Unbound | Analogue:BVD | Unbound | Unbound | |
| Reference for Active-site residues | ||
|---|---|---|
| resource | references | E.C. |
| literature [4], [5], [7], [10] | ||
| Active-site residues | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| PDB | Catalytic residues | Cofactor-binding residues | Modified residues | Main-chain involved in catalysis | Comment | |||||
| 1j90A00 |
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LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | deletion mutant 231-250 | |
| 1j90B00 |
|
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LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | deletion mutant 231-250 | |
| 1oe0A00 |
|
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LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | deletion mutant 231-250 | |
| 1oe0B00 |
|
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LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | deletion mutant 231-250 | |
| 1oe0C00 |
|
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LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | deletion mutant 231-250 | |
| 1oe0D00 |
|
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LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | deletion mutant 231-250 | |
| 1ot3A00 |
|
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LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | ||
| 1ot3B00 |
|
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LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | ||
| 1ot3C00 |
|
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LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | ||
| 1ot3D00 |
|
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LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | ||
| 1ot3E00 |
|
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LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | ||
| 1ot3F00 |
|
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LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | ||
| 1ot3G00 |
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LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | ||
| 1ot3H00 |
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LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | ||
| 1zm7A00 |
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LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | mutant N64D, deletion mutant 231-250 | |
| 1zm7B00 |
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LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | mutant N64D, deletion mutant 231-250 | |
| 1zm7C00 |
|
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LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | mutant N64D, deletion mutant 231-250 | |
| 1zm7D00 |
|
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LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | mutant N64D, deletion mutant 231-250 | |
| 1zmxA00 |
|
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LYS 33;GLU 52;ARG 105;ARG 167; | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | mutant N64D, deletion mutant 231-250, invisible 168-174 | |
| 1zmxB00 |
|
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LYS 33;GLU 52;ARG 105;ARG 167; | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | mutant N64D, deletion mutant 231-250, invisible 168-174, 195-199 | |
| 1zmxC00 |
|
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LYS 33;GLU 52;ARG 105;ARG 167; | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | mutant N64D, deletion mutant 231-250, invisible 168-173 | |
| 1zmxD00 |
|
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LYS 33;GLU 52;ARG 105;ARG 167; | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | mutant N64D, deletion mutant 231-250, invisible 168-174, 195-199 | |
| 1zmxE00 |
|
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LYS 33;GLU 52;ARG 105;ARG 167; | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | mutant N64D, deletion mutant 231-250, invisible 168-174 | |
| 1zmxF00 |
|
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LYS 33;GLU 52;ARG 105;ARG 167; | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | mutant N64D, deletion mutant 231-250, invisible 168-172, 195-199 | |
| 1zmxG00 |
|
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LYS 33;GLU 52;ARG 105;ARG 167; | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | mutant N64D, deletion mutant 231-250, invisible 168-174 | |
| 1zmxH00 |
|
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LYS 33;GLU 52;ARG 105;ARG 167; | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | mutant N64D, deletion mutant 231-250, invisible 168-173, 195-199 | |
| 2jcsA00 |
|
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LYS 33; ;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | mutant E52D, deletion mutant 231-250 | |
| 2jcsB00 |
|
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LYS 33; ;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | mutant E52D, deletion mutant 231-250 | |
| 2jj8A00 |
|
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LYS 33;GLU 52;ARG 105; ; | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | deletion mutant 231-250, invisible 164-173, 194-196 | |
| 2jj8B00 |
|
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LYS 33;GLU 52;ARG 105;ARG 167; | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | deletion mutant 231-250, invisible 168-173, 195-199 | |
| 2jj8C00 |
|
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LYS 33;GLU 52;ARG 105; ; | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | deletion mutant 231-250, invisible 165-175, 194-199 | |
| 2jj8D00 |
|
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|
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LYS 33;GLU 52;ARG 105;ARG 167; | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | deletion mutant 231-250, invisible 168-173, 195-199 | |
| 2vp0A00 |
|
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LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | ||
| 2vp0B00 |
|
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LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | ||
| 2vp2A00 |
|
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LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | deletion mutant 231-250 | |
| 2vp2B00 |
|
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LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | deletion mutant 231-250 | |
| 2vp4A00 |
|
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LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | deletion mutant 231-250 | |
| 2vp4B00 |
|
|
|
|
|
LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | deletion mutant 231-250 | |
| 2vp4C00 |
|
|
|
|
|
LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | deletion mutant 231-250 | |
| 2vp4D00 |
|
|
|
|
|
LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | deletion mutant 231-250 | |
| 2vp5A00 |
|
|
|
|
|
LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | deletion mutant 231-250 | |
| 2vp5B00 |
|
|
|
|
|
LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | deletion mutant 231-250, invisible 194-199 | |
| 2vp6A00 |
|
|
|
|
|
LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | deletion mutant 231-250 | |
| 2vp6B00 |
|
|
|
|
|
LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | deletion mutant 231-250, invisible 195-199 | |
| 2vp6C00 |
|
|
|
|
|
LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | deletion mutant 231-250 | |
| 2vp6D00 |
|
|
|
|
|
LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | deletion mutant 231-250, invisible 194-199 | |
| 2vp6E00 |
|
|
|
|
|
LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | deletion mutant 231-250, invisible 195-199 | |
| 2vp6F00 |
|
|
|
|
|
LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | deletion mutant 231-250, invisible 195-199 | |
| 2vp6G00 |
|
|
|
|
|
LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | deletion mutant 231-250, invisible 195-199 | |
| 2vp6H00 |
|
|
|
|
|
LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | deletion mutant 231-250, invisible 195-199 | |
| 2vp9A00 |
|
|
|
|
|
LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | deletion mutant 231-250 | |
| 2vp9B00 |
|
|
|
|
|
LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | deletion mutant 231-250, invisible 195-199 | |
| 2vp9C00 |
|
|
|
|
|
LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | deletion mutant 231-250 | |
| 2vp9D00 |
|
|
|
|
|
LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | deletion mutant 231-250, invisible 194-199 | |
| 2vp9E00 |
|
|
|
|
|
LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | deletion mutant 231-250, invisible 195-199 | |
| 2vp9F00 |
|
|
|
|
|
LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | deletion mutant 231-250, invisible 195-199 | |
| 2vp9G00 |
|
|
|
|
|
LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | deletion mutant 231-250, invisible 195-199 | |
| 2vp9H00 |
|
|
|
|
|
LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | deletion mutant 231-250, invisible 195-199 | |
| 2vppA00 |
|
|
|
|
|
LYS 33;GLU 52;ARG 105;ARG 167;ARG 169 | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | deletion mutant 231-250, invisible 198-200 | |
| 2vppB00 |
|
|
|
|
|
LYS 33;GLU 52;ARG 105;ARG 167; | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | deletion mutant 231-250, invisible 168-169, 198-200 | |
| 2vqsA00 |
|
|
|
|
|
LYS 33;GLU 52;ARG 105; ; | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | deletion mutant 231-250, invisible 164-175, 194-196 | |
| 2vqsB00 |
|
|
|
|
|
LYS 33;GLU 52;ARG 105;ARG 167; | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | deletion mutant 231-250, invisible 168-173, 194-196 | |
| 2vqsC00 |
|
|
|
|
|
LYS 33;GLU 52;ARG 105; ; | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | deletion mutant 231-250, invisible 167-175, 194-195 | |
| 2vqsD00 |
|
|
|
|
|
LYS 33;GLU 52;ARG 105; ; | THR 34;GLU 104(Magnesium binding) | GLY 30;SER 31;GLY 32;LYS 33 | deletion mutant 231-250, invisible 165-173, 195-199 | |
| References for Catalytic Mechanism | ||
|---|---|---|
| References | Sections | No. of steps in catalysis |
|
[4]
|
p.617-618 | |
|
[5]
|
Fig.7, p.1338-1339 | |
|
[7]
|
p.5710, p.5711 | |
|
[10]
|
p.1544-1548 | |
| References | |
|---|---|
| [1] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.75 ANGSTROMS) OF 34-376. |
| Medline ID | |
| PubMed ID | 9336833 |
| Journal | Protein Sci |
| Year | 1997 |
| Volume | 6 |
| Pages | 2097-106 |
| Authors | Wild K, Bohner T, Folkers G, Schulz GE |
| Title | The structures of thymidine kinase from herpes simplex virus type 1 in complex with substrates and a substrate analogue. |
| Related PDB | 1vtk 2vtk 3vtk |
| Related UniProtKB | P03176 |
| [2] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS). |
| Medline ID | |
| PubMed ID | 9715911 |
| Journal | Proteins |
| Year | 1998 |
| Volume | 32 |
| Pages | 350-61 |
| Authors | Champness JN, Bennett MS, Wien F, Visse R, Summers WC, Herdewijn P, de Clerq E, Ostrowski T, Jarvest RL, Sanderson MR |
| Title | Exploring the active site of herpes simplex virus type-1 thymidine kinase by X-ray crystallography of complexes with aciclovir and other ligands. |
| Related PDB | 1kim |
| Related UniProtKB | |
| [3] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10692477 |
| Journal | J Biol Chem |
| Year | 2000 |
| Volume | 275 |
| Pages | 6673-9 |
| Authors | Munch-Petersen B, Knecht W, Lenz C, Sondergaard L, Piskur J |
| Title | Functional expression of a multisubstrate deoxyribonucleoside kinase from Drosophila melanogaster and its C-terminal deletion mutants. |
| Related PDB | |
| Related UniProtKB | Q9XZT6 |
| [4] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11427893 |
| Journal | Nat Struct Biol |
| Year | 2001 |
| Volume | 8 |
| Pages | 616-20 |
| Authors | Johansson K, Ramaswamy S, Ljungcrantz C, Knecht W, Piskur J, Munch-Petersen B, Eriksson S, Eklund H |
| Title | Structural basis for substrate specificities of cellular deoxyribonucleoside kinases. |
| Related PDB | 1j90 1jag |
| Related UniProtKB | |
| [5] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12363036 |
| Journal | Cell Mol Life Sci |
| Year | 2002 |
| Volume | 59 |
| Pages | 1327-46 |
| Authors | Eriksson S, Munch-Petersen B, Johansson K, Eklund H |
| Title | Structure and function of cellular deoxyribonucleoside kinases. |
| Related PDB | |
| Related UniProtKB | |
| [6] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11927571 |
| Journal | EMBO J |
| Year | 2002 |
| Volume | 21 |
| Pages | 1873-80 |
| Authors | Knecht W, Sandrini MP, Johansson K, Eklund H, Munch-Petersen B, Piskur J |
| Title | A few amino acid substitutions can convert deoxyribonucleoside kinase specificity from pyrimidines to purines. |
| Related PDB | |
| Related UniProtKB | |
| [7] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12741827 |
| Journal | Biochemistry |
| Year | 2003 |
| Volume | 42 |
| Pages | 5706-12 |
| Authors | Mikkelsen NE, Johansson K, Karlsson A, Knecht W, Andersen G, Piskur J, Munch-Petersen B, Eklund H |
| Title | Structural basis for feedback inhibition of the deoxyribonucleoside salvage pathway: studies of the Drosophila deoxyribonucleoside kinase. |
| Related PDB | 1oe0 1ot3 |
| Related UniProtKB | |
| [8] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12823558 |
| Journal | Eur J Biochem |
| Year | 2003 |
| Volume | 270 |
| Pages | 2879-84 |
| Authors | Solaroli N, Bjerke M, Amiri MH, Johansson M, Karlsson A |
| Title | Active site mutants of Drosophila melanogaster multisubstrate deoxyribonucleoside kinase. |
| Related PDB | |
| Related UniProtKB | |
| [9] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 16008571 |
| Journal | FEBS J |
| Year | 2005 |
| Volume | 272 |
| Pages | 3733-42 |
| Authors | Welin M, Skovgaard T, Knecht W, Zhu C, Berenstein D, Munch-Petersen B, Piskur J, Eklund H |
| Title | Structural basis for the changed substrate specificity of Drosophila melanogaster deoxyribonucleoside kinase mutant N64D. |
| Related PDB | 1zm7 1zmx |
| Related UniProtKB | |
| [10] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 17302737 |
| Journal | FEBS J |
| Year | 2007 |
| Volume | 274 |
| Pages | 1542-51 |
| Authors | Egeblad-Welin L, Sonntag Y, Eklund H, Munch-Petersen B |
| Title |
Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase. |
| Related PDB | 2jcs |
| Related UniProtKB | |
| [11] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 18384378 |
| Journal | FEBS J |
| Year | 2008 |
| Volume | 275 |
| Pages | 2151-60 |
| Authors | Mikkelsen NE, Munch-Petersen B, Eklund H |
| Title | Structural studies of nucleoside analog and feedback inhibitor binding to Drosophila melanogaster multisubstrate deoxyribonucleoside kinase. |
| Related PDB | 2jj8 2vp0 2vp2 2vp4 2vp5 2vp6 2vp9 2vqs |
| Related UniProtKB | |
| [12] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 19285960 |
| Journal | Biochem Biophys Res Commun |
| Year | 2009 |
| Volume | 382 |
| Pages | 430-3 |
| Authors | Knecht W, Mikkelsen NE, Clausen AR, Willer M, Eklund H, Gojkovi? Z, Piskur J |
| Title | Drosophila melanogaster deoxyribonucleoside kinase activates gemcitabine. |
| Related PDB | 2vpp |
| Related UniProtKB | |
| Comments |
|---|
|
This enzyme is closely related to deoxycytidine kinase (EC 2.7.1.74; S00682 in EzCatDB), According to the literature [5] and [7], |
| Created | Updated |
|---|---|
| 2009-09-10 | 2012-03-02 |