DB code: S00345
| RLCP classification | 1.12.30000.10 : Hydrolysis | |
|---|---|---|
| CATH domain | 3.40.50.1820 : Rossmann fold | Catalytic domain |
| E.C. | 3.1.1.1 | |
| CSA | 1auo | |
| M-CSA | 1auo | |
| MACiE | ||
| CATH domain | Related DB codes (homologues) |
|---|---|
| 3.40.50.1820 : Rossmann fold | S00544 S00344 S00517 S00525 S00526 S00720 S00723 S00724 S00725 S00919 S00057 S00374 S00347 S00348 S00346 S00350 S00352 S00353 S00355 S00356 S00358 D00189 D00210 D00539 T00253 |
| Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | Pfam |
|---|---|---|---|
| Q53547 |
Carboxylesterase 2
|
EC
3.1.1.1
Esterase II |
PF02230
(Abhydrolase_2)
[Graphical View] |
| KEGG enzyme name |
|---|
|
carboxylesterase
ali-esterase B-esterase monobutyrase cocaine esterase procaine esterase methylbutyrase vitamin A esterase butyryl esterase carboxyesterase carboxylate esterase carboxylic esterase methylbutyrate esterase triacetin esterase carboxyl ester hydrolase butyrate esterase methylbutyrase alpha-carboxylesterase propionyl esterase nonspecific carboxylesterase esterase D esterase B esterase A serine esterase carboxylic acid esterase cocaine esterase |
| UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
|---|---|---|---|---|---|
| Q53547 | EST2_PSEFL | A carboxylic ester + H(2)O = an alcohol + a carboxylate. | Homodimer. |
| KEGG Pathways | Map code | Pathways | E.C. |
|---|---|---|
| MAP00960 | Alkaloid biosynthesis II | |
| MAP00983 | Drug metabolism - other enzymes |
| Compound table | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Substrates | Products | Intermediates | ||||||||||
| KEGG-id | C02391 | C00001 | C00069 | C00060 | I00123 | I00085 | I00086 | |||||
| E.C. | ||||||||||||
| Compound | Carboxylic ester | H2O | Alcohol | Carboxylate | Peptidyl-Ser-tetrahedral intermediate (with previous carboxylic-ester) | Acyl-enzyme(Peptidyl-Ser-acyl group) | Peptidyl-Ser-tetrahedral-intermediate | |||||
| Type | carbohydrate | H2O | carbohydrate | carboxyl group | ||||||||
| ChEBI |
15377 15377 |
|||||||||||
| PubChem |
22247451 962 22247451 962 |
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| 1auoA |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1auoB |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1aurA |
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|
Unbound | Unbound | Unbound | Unbound | Unbound | Analogue:PMS | |
| 1aurB |
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Unbound | Unbound | Unbound | Unbound | Unbound | Analogue:PMS | |
| Reference for Active-site residues | ||
|---|---|---|
| resource | references | E.C. |
| Swiss-prot, PDB & literature | ||
| Active-site residues | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| PDB | Catalytic residues | Cofactor-binding residues | Modified residues | Main-chain involved in catalysis | Comment | |||||
| 1auoA |
|
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|
|
|
SER 114;ASP 168;HIS 199 | LEU 23;GLN 115 | |||
| 1auoB |
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|
|
SER 114;ASP 168;HIS 199 | LEU 23;GLN 115 | |||
| 1aurA |
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|
SER 114;ASP 168;HIS 199 | LEU 23;GLN 115 | |||
| 1aurB |
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|
|
|
SER 114;ASP 168;HIS 199 | LEU 23;GLN 115 | |||
| References for Catalytic Mechanism | ||
|---|---|---|
| References | Sections | No. of steps in catalysis |
|
[1]
|
p.1574-1576 | |
|
[2]
|
p.762-763 | |
|
[3]
|
p.12300-12301 | |
| References | |
|---|---|
| [1] | |
| Resource | |
| Comments | X-ray crystallography (1.8 Angstroms) |
| Medline ID | |
| PubMed ID | 9438866 |
| Journal | Structure |
| Year | 1997 |
| Volume | 5 |
| Pages | 1571-84 |
| Authors | Kim KK, Song HK, Shin DH, Hwang KY, Choe S, Yoo OJ, Suh SW |
| Title |
Crystal structure of carboxylesterase from Pseudomonas fluorescens, |
| Related PDB | 1auo 1aur |
| Related UniProtKB | Q53547 |
| [2] | |
| Resource | |
| Comments | X-ray crystallography (2.6 Angstroms) |
| Medline ID | |
| PubMed ID | 11061974 |
| Journal | J Mol Biol |
| Year | 2000 |
| Volume | 303 |
| Pages | 761-71 |
| Authors | De Simone G, Galdiero S, Manco G, Lang D, Rossi M, Pedone C |
| Title | A snapshot of a transition state analogue of a novel thermophilic esterase belonging to the subfamily of mammalian hormone-sensitive lipase. |
| Related PDB | 1evq |
| Related UniProtKB | |
| [3] | |
| Resource | |
| Comments | Homologous enzyme |
| Medline ID | |
| PubMed ID | 12369817 |
| Journal | Biochemistry |
| Year | 2002 |
| Volume | 41 |
| Pages | 12297-307 |
| Authors | Turner JM, Larsen NA, Basran A, Barbas CF 3rd, Bruce NC, Wilson IA, Lerner RA |
| Title | Biochemical characterization and structural analysis of a highly proficient cocaine esterase. |
| Related PDB | |
| Related UniProtKB | |
| Comments |
|---|
| Created | Updated |
|---|---|
| 2002-07-11 | 2012-10-22 |