DB code: T00211

CATH domain 3.90.660.10 : Polyamine Oxidase; Chain A, domain 2 Catalytic domain
3.50.50.60 : FAD/NAD(P)-binding domain
1.10.405.10 : Guanine Nucleotide Dissociation Inhibitor; domain 1 Catalytic domain
E.C. 1.4.3.2
CSA 1f8r
M-CSA 1f8r
MACiE

CATH domain Related DB codes (homologues)
3.50.50.60 : FAD/NAD(P)-binding domain M00163 D00015 D00041 D00042 D00045 D00064 D00071 T00004 T00015 T00017 T00025 T00213 T00233 T00242
3.90.660.10 : Polyamine Oxidase; Chain A, domain 2 D00042

Uniprot Enzyme Name
UniprotKB Protein name Synonyms Pfam
P81382 L-amino-acid oxidase
LAAO
LAO
EC 1.4.3.2
PF01593 (Amino_oxidase)
[Graphical View]

KEGG enzyme name
L-amino-acid oxidase
ophio-amino-acid oxidase

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P81382 OXLA_AGKRH An L-amino acid + H(2)O + O(2) = a 2-oxo acid + NH(3) + H(2)O(2). Homodimer. Secreted. FAD.

KEGG Pathways
Map code Pathways E.C.
MAP00252 Alanine and aspartate metabolism
MAP00271 Methionine metabolism
MAP00280 Valine, leucine and isoleucine degradation
MAP00350 Tyrosine metabolism
MAP00360 Phenylalanine metabolism
MAP00380 Tryptophan metabolism
MAP00400 Phenylalanine, tyrosine and tryptophan biosynthesis
MAP00950 Alkaloid biosynthesis I

Compound table
Cofactors Substrates Products Intermediates
KEGG-id C00016 C00001 C00007 C00151 C00014 C00027 C00161
E.C.
Compound FAD H2O O2 L-Amino acid NH3 H2O2 2-Oxo acid
Type amide group,amine group,aromatic ring (only carbon atom),aromatic ring (with nitrogen atoms),carbohydrate,nucleotide H2O others amino acids amine group,organic ion others carbohydrate,carboxyl group
ChEBI 16238
 16238
 		15377
 15377
 		15379
 26689
 27140
 15379
 26689
 27140
 		16134
 16134
 		16240
 16240
PubChem 643975
 643975
 		22247451
 962
 22247451
 962
 		977
 977
 		222
 222
 		22326046
 784
 22326046
 784
1f8rA01 Unbound Unbound Unbound Unbound Unbound Analogue:CIT
1f8rB01 Unbound Unbound Unbound Unbound Unbound Analogue:CIT
1f8rC01 Unbound Unbound Unbound Unbound Unbound Analogue:CIT
1f8rD01 Unbound Unbound Unbound Unbound Unbound Analogue:CIT
1f8sA01 Unbound Unbound Analogue:BE2_1 Unbound Unbound Unbound
1f8sB01 Unbound Unbound Analogue:BE2_4 Unbound Unbound Unbound
1f8sC01 Unbound Unbound Analogue:BE2_7 Unbound Unbound Unbound
1f8sD01 Unbound Unbound Analogue:BE2_10 Unbound Unbound Unbound
1f8sE01 Unbound Unbound Analogue:BE2_13 Unbound Unbound Unbound
1f8sF01 Unbound Unbound Analogue:BE2_16 Unbound Unbound Unbound
1f8sG01 Unbound Unbound Analogue:BE2_19 Unbound Unbound Unbound
1f8sH01 Unbound Unbound Analogue:BE2_22 Unbound Unbound Unbound
1tdkA01 Unbound Unbound Bound:LVG_491 Unbound Unbound Unbound
1tdnA01 Unbound Unbound Bound:LEU Unbound Unbound Unbound
1tdoA01 Unbound Unbound Bound:PHE Unbound Unbound Unbound
1reoA01 Unbound Unbound Unbound Unbound Unbound Analogue:CIT
1f8rA02 Bound:FAD Unbound Unbound Unbound Unbound Unbound
1f8rB02 Bound:FAD Unbound Unbound Unbound Unbound Unbound
1f8rC02 Bound:FAD Unbound Unbound Unbound Unbound Unbound
1f8rD02 Bound:FAD Unbound Unbound Unbound Unbound Unbound
1f8sA02 Bound:FAD Unbound Unbound Unbound Unbound Unbound
1f8sB02 Bound:FAD Unbound Unbound Unbound Unbound Unbound
1f8sC02 Bound:FAD Unbound Unbound Unbound Unbound Unbound
1f8sD02 Bound:FAD Unbound Unbound Unbound Unbound Unbound
1f8sE02 Bound:FAD Unbound Unbound Unbound Unbound Unbound
1f8sF02 Bound:FAD Unbound Unbound Unbound Unbound Unbound
1f8sG02 Bound:FAD Unbound Unbound Unbound Unbound Unbound
1f8sH02 Bound:FAD Unbound Unbound Unbound Unbound Unbound
1tdkA02 Bound:FAD Unbound Unbound Unbound Unbound Unbound
1tdnA02 Bound:FAD Unbound Unbound Unbound Unbound Unbound
1tdoA02 Bound:FAD Unbound Unbound Unbound Unbound Unbound
1reoA02 Bound:FAD Unbound Unbound Unbound Unbound Unbound
1f8rA03 Unbound Unbound Unbound Unbound Unbound Unbound
1f8rB03 Unbound Unbound Unbound Unbound Unbound Unbound
1f8rC03 Unbound Unbound Unbound Unbound Unbound Unbound
1f8rD03 Unbound Unbound Unbound Unbound Unbound Unbound
1f8sA03 Unbound Unbound Unbound Unbound Unbound Unbound
1f8sB03 Unbound Unbound Unbound Unbound Unbound Unbound
1f8sC03 Unbound Unbound Unbound Unbound Unbound Unbound
1f8sD03 Unbound Unbound Unbound Unbound Unbound Unbound
1f8sE03 Unbound Unbound Unbound Unbound Unbound Unbound
1f8sF03 Unbound Unbound Unbound Unbound Unbound Unbound
1f8sG03 Unbound Unbound Unbound Unbound Unbound Unbound
1f8sH03 Unbound Unbound Unbound Unbound Unbound Unbound
1tdkA03 Unbound Unbound Unbound Unbound Unbound Unbound
1tdnA03 Unbound Unbound Unbound Unbound Unbound Unbound
1tdoA03 Unbound Unbound Unbound Unbound Unbound Unbound
1reoA03 Unbound Unbound Unbound Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.
literature [5]

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1f8rA01 LYS 326
1f8rB01 LYS 326
1f8rC01 LYS 326
1f8rD01 LYS 326
1f8sA01 LYS 326
1f8sB01 LYS 326
1f8sC01 LYS 326
1f8sD01 LYS 326
1f8sE01 LYS 326
1f8sF01 LYS 326
1f8sG01 LYS 326
1f8sH01 LYS 326
1tdkA01 LYS 326
1tdnA01 LYS 326
1tdoA01 LYS 326
1reoA01 LYS 326
1f8rA02
1f8rB02
1f8rC02
1f8rD02
1f8sA02
1f8sB02
1f8sC02
1f8sD02
1f8sE02
1f8sF02
1f8sG02
1f8sH02
1tdkA02
1tdnA02
1tdoA02
1reoA02
1f8rA03 HIS 223
1f8rB03 HIS 223
1f8rC03 HIS 223
1f8rD03 HIS 223
1f8sA03 HIS 223
1f8sB03 HIS 223
1f8sC03 HIS 223
1f8sD03 HIS 223
1f8sE03 HIS 223
1f8sF03 HIS 223
1f8sG03 HIS 223
1f8sH03 HIS 223
1tdkA03 HIS 223
1tdnA03 HIS 223
1tdoA03 HIS 223
1reoA03 HIS 223

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[5]
Scheme 1, p.4212-4213 2
[7]
Scheme 1
[8]
Scheme 1
[11]
Fig.1, Fig.4, p.23975-23976

References
[1]
Resource
Comments
Medline ID
PubMed ID 2044840
Journal Int J Biochem
Year 1991
Volume 23
Pages 323-7
Authors Tan NH, Saifuddin MN
Title Substrate specificity of king cobra (Ophiophagus hannah) venom L-amino acid oxidase.
Related PDB
Related UniProtKB
[2]
Resource
Comments
Medline ID
PubMed ID 8755511
Journal Proc Natl Acad Sci U S A
Year 1996
Volume 93
Pages 7546-51
Authors Raibekas AA, Massey V
Title Glycerol-induced development of catalytically active conformation of Crotalus adamanteus L-amino acid oxidase in vitro.
Related PDB
Related UniProtKB
[3]
Resource
Comments
Medline ID
PubMed ID 10441379
Journal Arch Biochem Biophys
Year 1999
Volume 368
Pages 285-90
Authors Souza DH, Eugenio LM, Fletcher JE, Jiang MS, Garratt RC, Oliva G, Selistre-de-Araujo HS
Title Isolation and structural characterization of a cytotoxic L-amino acid oxidase from Agkistrodon contortrix laticinctus snake venom: preliminary crystallographic data.
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID 11368308
Journal Arch Biochem Biophys
Year 2000
Volume 384
Pages 216-26
Authors Ali SA, Stoeva S, Abbasi A, Alam JM, Kayed R, Faigle M, Neumeister B, Voelter W
Title Isolation, structural, and functional characterization of an apoptosis-inducing L-amino acid oxidase from leaf-nosed viper (Eristocophis macmahoni) snake venom.
Related PDB
Related UniProtKB
[5]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS), AND CARBOHYDRATE-LINKAGE SITES
Medline ID 20402326
PubMed ID 10944103
Journal EMBO J
Year 2000
Volume 19
Pages 4204-15
Authors Pawelek PD, Cheah J, Coulombe R, Macheroux P, Ghisla S, Vrielink A
Title The structure of L-amino acid oxidase reveals the substrate trajectory into an enantiomerically conserved active site.
Related PDB 1f8r 1f8s
Related UniProtKB P81382
[6]
Resource
Comments
Medline ID
PubMed ID 11341935
Journal Biochim Biophys Acta
Year 2001
Volume 1544
Pages 267-77
Authors Takatsuka H, Sakurai Y, Yoshioka A, Kokubo T, Usami Y, Suzuki M, Matsui T, Titani K, Yagi H, Matsumoto M, Fujimura Y
Title Molecular characterization of L-amino acid oxidase from Agkistrodon halys blomhoffii with special reference to platelet aggregation.
Related PDB
Related UniProtKB
[7]
Resource
Comments
Medline ID
PubMed ID 11453999
Journal Eur J Biochem
Year 2001
Volume 268
Pages 4044-53
Authors Geyer A, Fitzpatrick TB, Pawelek PD, Kitzing K, Vrielink A, Ghisla S, Macheroux P
Title Structure and characterization of the glycan moiety of L-amino-acid oxidase from the Malayan pit viper Calloselasma rhodostoma.
Related PDB
Related UniProtKB
[8]
Resource
Comments
Medline ID
PubMed ID 11248687
Journal Eur J Biochem
Year 2001
Volume 268
Pages 1679-86
Authors MacHeroux P, Seth O, Bollschweiler C, Schwarz M, Kurfurst M, Au LC, Ghisla S
Title L-amino-acid oxidase from the Malayan pit viper Calloselasma rhodostoma. Comparative sequence analysis and characterization of active and inactive forms of the enzyme.
Related PDB
Related UniProtKB
[9]
Resource
Comments
Medline ID
PubMed ID 12031486
Journal Biochim Biophys Acta
Year 2002
Volume 1576
Pages 70-80
Authors Chavan SS, Tian W, Hsueh K, Jawaheer D, Gregersen PK, Chu CC
Title Characterization of the human homolog of the IL-4 induced gene-1 (Fig1).
Related PDB
Related UniProtKB
[10]
Resource
Comments
Medline ID
PubMed ID
Journal Enzyme Microb Technol
Year 2002
Volume 31
Pages 77-87
Authors Geueke B, Hummel W
Title A new bacterial L-amino acid oxidase with a broad substrate specificity: purification and characterization.
Related PDB
Related UniProtKB
[11]
Resource
Comments
Medline ID
PubMed ID 12015330
Journal J Biol Chem
Year 2002
Volume 277
Pages 23973-6
Authors Binda C, Mattevi A, Edmondson DE
Title Structure-function relationships in flavoenzyme-dependent amine oxidations: a comparison of polyamine oxidase and monoamine oxidase.
Related PDB
Related UniProtKB
[12]
Resource
Comments
Medline ID
PubMed ID 12175601
Journal Toxicon
Year 2002
Volume 40
Pages 659-65
Authors Du XY, Clemetson KJ
Title Snake venom L-amino acid oxidases.
Related PDB
Related UniProtKB
[13]
Resource
Comments
Medline ID
PubMed ID 14636049
Journal Biochemistry
Year 2003
Volume 42
Pages 13826-32
Authors Sobrado P, Fitzpatrick PF
Title Analysis of the role of the active site residue Arg98 in the flavoprotein tryptophan 2-monooxygenase, a member of the L-amino oxidase family.
Related PDB
Related UniProtKB
[14]
Resource
Comments
Medline ID
PubMed ID 15103157
Journal Acta Crystallogr D Biol Crystallogr
Year 2004
Volume 60
Pages 974-7
Authors Zhang H, Teng M, Niu L, Wang Y, Wang Y, Liu Q, Huang Q, Hao Q, Dong Y, Liu P
Title Purification, partial characterization, crystallization and structural determination of AHP-LAAO, a novel L-amino-acid oxidase with cell apoptosis-inducing activity from Agkistrodon halys pallas venom.
Related PDB 1tdk 1tdn 1tdo 1reo
Related UniProtKB

Comments
This enzyme catalyzes three distinct reactions (see [7], [8] & [11]):
(A) Hydride transfer from amine group to FAD(ox)
(B) Exchange of double-bonded atoms (Schiff-base deformation)
(C) Hydride transfer from FADH2(red) to O2

Created Updated
2004-06-18 2009-02-26