DB code: S00302
| RLCP classification | 3.103.70000.350 : Transfer | |
|---|---|---|
| CATH domain | 3.40.50.300 : Rossmann fold | Catalytic domain |
| E.C. | 2.7.1.21 | |
| CSA | 1kim | |
| M-CSA | 1kim | |
| MACiE | ||
| CATH domain | Related DB codes (homologues) |
|---|---|
| 3.40.50.300 : Rossmann fold | S00527 S00547 S00548 S00550 S00554 S00555 S00671 S00672 S00676 S00680 S00682 S00913 S00914 S00301 S00303 S00304 S00307 S00308 S00305 S00306 S00309 S00310 S00311 M00114 M00199 D00129 D00130 D00540 M00186 |
| Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | RefSeq | Pfam |
|---|---|---|---|---|
| P03176 |
Thymidine kinase
|
EC
2.7.1.21
|
NP_044624.1
(Protein)
NC_001806.1 (DNA/RNA sequence) |
PF00693
(Herpes_TK)
[Graphical View] |
| P06479 |
Thymidine kinase
|
EC
2.7.1.21
|
PF00693
(Herpes_TK)
[Graphical View] |
|
| P24425 |
Thymidine kinase
|
EC
2.7.1.21
|
PF00693
(Herpes_TK)
[Graphical View] |
|
| P0C0E6 |
Thymidine kinase
|
EC
2.7.1.21
|
PF00693
(Herpes_TK)
[Graphical View] |
| KEGG enzyme name |
|---|
|
thymidine kinase
thymidine kinase (phosphorylating) 2'-deoxythymidine kinase deoxythymidine kinase (phosphorylating) |
| UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
|---|---|---|---|---|---|
| P03176 | KITH_HHV11 | ATP + thymidine = ADP + thymidine 5''- phosphate. | Homodimer. | ||
| P06479 | KITH_HHV1S | ATP + thymidine = ADP + thymidine 5''- phosphate. | Homodimer (By similarity). | ||
| P24425 | KITH_EHV4 | ATP + thymidine = ADP + thymidine 5''- phosphate. | |||
| P0C0E6 | KITH_VZVO | ATP + thymidine = ADP + thymidine 5''- phosphate. |
| KEGG Pathways | Map code | Pathways | E.C. |
|---|---|---|
| MAP00240 | Pyrimidine metabolism | |
| MAP00983 | Drug metabolism - other enzymes |
| Compound table | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Cofactors | Substrates | Products | Intermediates | ||||||||
| KEGG-id | C00305 | C00002 | C00214 | C00008 | C00364 | ||||||
| E.C. | |||||||||||
| Compound | Magnesium | ATP | Thymidine | ADP | Thymidine 5'-phosphate | ||||||
| Type | divalent metal (Ca2+, Mg2+) | amine group,nucleotide | amide group,nucleoside | amine group,nucleotide | amide group,nucleotide | ||||||
| ChEBI |
18420 18420 |
15422 15422 |
17748 17748 |
16761 16761 |
17013 17013 |
||||||
| PubChem |
888 888 |
5957 5957 |
5789 5789 |
6022 6022 |
9700 9700 |
||||||
| 1e2hA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1e2hB |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1e2iA |
|
|
|
|
|
Unbound | Unbound | Analogue:APS-APS-ARP-ARP | Unbound | Unbound | |
| 1e2iB |
|
|
|
|
|
Unbound | Unbound | Analogue:APS-ARP-APS-ARP | Unbound | Unbound | |
| 1e2jA |
|
|
|
|
|
Unbound | Unbound | Bound:THM | Unbound | Unbound | |
| 1e2jB |
|
|
|
|
|
Unbound | Unbound | Bound:THM | Unbound | Unbound | |
| 1e2kA |
|
|
|
|
|
Unbound | Unbound | Analogue:TMC | Unbound | Unbound | |
| 1e2kB |
|
|
|
|
|
Unbound | Unbound | Analogue:TMC | Unbound | Unbound | |
| 1e2lA |
|
|
|
|
|
Unbound | Unbound | Analogue:TMC | Unbound | Unbound | |
| 1e2lB |
|
|
|
|
|
Unbound | Unbound | Analogue:TMC | Unbound | Unbound | |
| 1e2mA |
|
|
|
|
|
Unbound | Unbound | Analogue:HPT | Unbound | Unbound | |
| 1e2mB |
|
|
|
|
|
Unbound | Unbound | Analogue:HPT | Unbound | Unbound | |
| 1e2nA |
|
|
|
|
|
Unbound | Unbound | Analogue:RCA | Unbound | Unbound | |
| 1e2nB |
|
|
|
|
|
Unbound | Unbound | Analogue:RCA | Unbound | Unbound | |
| 1e2pA |
|
|
|
|
|
Unbound | Unbound | Analogue:CCV | Unbound | Unbound | |
| 1e2pB |
|
|
|
|
|
Unbound | Unbound | Analogue:CCV | Unbound | Unbound | |
| 1ki2A |
|
|
|
|
|
Unbound | Unbound | Analogue:GA2 | Unbound | Unbound | |
| 1ki2B |
|
|
|
|
|
Unbound | Unbound | Analogue:GA2 | Unbound | Unbound | |
| 1ki3A |
|
|
|
|
|
Unbound | Unbound | Analogue:PE2 | Unbound | Unbound | |
| 1ki3B |
|
|
|
|
|
Unbound | Unbound | Analogue:PE2 | Unbound | Unbound | |
| 1ki4A |
|
|
|
|
|
Unbound | Unbound | Analogue:BTD | Unbound | Unbound | |
| 1ki4B |
|
|
|
|
|
Unbound | Unbound | Analogue:BTD | Unbound | Unbound | |
| 1ki5A |
|
|
|
|
|
Unbound | Unbound | Analogue:AC2 | Unbound | Unbound | |
| 1ki5B |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ki6A |
|
|
|
|
|
Unbound | Unbound | Analogue:AHU | Unbound | Unbound | |
| 1ki6B |
|
|
|
|
|
Unbound | Unbound | Analogue:AHU | Unbound | Unbound | |
| 1ki7A |
|
|
|
|
|
Unbound | Unbound | Analogue:ID2 | Unbound | Unbound | |
| 1ki7B |
|
|
|
|
|
Unbound | Unbound | Analogue:ID2 | Unbound | Unbound | |
| 1ki8A |
|
|
|
|
|
Unbound | Unbound | Analogue:BVD | Unbound | Unbound | |
| 1ki8B |
|
|
|
|
|
Unbound | Unbound | Analogue:BVD | Unbound | Unbound | |
| 1kimA |
|
|
|
|
|
Unbound | Unbound | Bound:THM | Unbound | Unbound | |
| 1kimB |
|
|
|
|
|
Unbound | Unbound | Bound:THM | Unbound | Unbound | |
| 1kinA |
|
|
|
|
|
Unbound | Unbound | Bound:THM | Unbound | Unbound | |
| 1kinB |
|
|
|
|
|
Unbound | Unbound | Bound:THM | Unbound | Unbound | |
| 1of1A |
|
|
|
|
|
Unbound | Unbound | Analogue:SCT | Unbound | Unbound | |
| 1of1B |
|
|
|
|
|
Unbound | Unbound | Analogue:SCT | Unbound | Unbound | |
| 1p7cA |
|
|
|
|
|
Unbound | Analogue:T5A(ATP) | Unbound | Unbound | Analogue:T5A(TMP) | |
| 1p7cB |
|
|
|
|
|
Unbound | Unbound | Bound:THM | Unbound | Unbound | |
| 1qhiA |
|
|
|
|
|
Unbound | Unbound | Analogue:BPG | Unbound | Unbound | |
| 1qhiB |
|
|
|
|
|
Unbound | Unbound | Analogue:BPG | Unbound | Unbound | |
| 1vtkA |
|
|
|
|
|
Unbound | Unbound | Unbound | Bound:ADP | Bound:TMP | |
| 2ki5A |
|
|
|
|
|
Unbound | Unbound | Analogue:AC2 | Unbound | Unbound | |
| 2ki5B |
|
|
|
|
|
Unbound | Unbound | Analogue:AC2 | Unbound | Unbound | |
| 2vtkA |
|
|
|
|
|
Unbound | Unbound | Bound:THM | Bound:ADP | Unbound | |
| 3vtkA |
|
|
|
|
|
Unbound | Unbound | Unbound | Bound:ADP | Analogue:5IU | |
| 1osnA |
|
|
|
|
|
Unbound | Unbound | Unbound | Bound:ADP | Analogue:BVP | |
| 1osnB |
|
|
|
|
|
Unbound | Unbound | Unbound | Bound:ADP | Analogue:BVP | |
| 1osnC |
|
|
|
|
|
Unbound | Unbound | Unbound | Bound:ADP | Analogue:BVP | |
| 1osnD |
|
|
|
|
|
Unbound | Unbound | Unbound | Bound:ADP | Analogue:BVP | |
| 1p6xA |
|
|
|
|
|
Unbound | Unbound | Bound:THM | Unbound | Unbound | |
| 1p6xB |
|
|
|
|
|
Unbound | Unbound | Bound:THM | Unbound | Unbound | |
| 1p72A |
|
|
|
|
|
Unbound | Unbound | Bound:THM | Bound:ADP | Unbound | |
| 1p72B |
|
|
|
|
|
Unbound | Unbound | Bound:THM | Bound:ADP | Unbound | |
| 1p73A |
|
|
|
|
|
Unbound | Analogue:4TA(ATP) | Unbound | Unbound | Analogue:4TA(TMP) | |
| 1p73B |
|
|
|
|
|
Unbound | Analogue:4TA(ATP) | Unbound | Unbound | Analogue:4TA(TMP) | |
| 1p73C |
|
|
|
|
|
Unbound | Analogue:4TA(ATP) | Unbound | Unbound | Analogue:4TA(TMP) | |
| 1p73D |
|
|
|
|
|
Unbound | Analogue:4TA(ATP) | Unbound | Unbound | Analogue:4TA(TMP) | |
| 1p75A |
|
|
|
|
|
Unbound | Analogue:T5A(ATP) | Unbound | Unbound | Analogue:T5A(TMP) | |
| 1p75B |
|
|
|
|
|
Unbound | Analogue:T5A(ATP) | Unbound | Unbound | Analogue:T5A(TMP) | |
| 1p75C |
|
|
|
|
|
Unbound | Analogue:T5A(ATP) | Unbound | Unbound | Analogue:T5A(TMP) | |
| 1p75D |
|
|
|
|
|
Unbound | Analogue:T5A(ATP) | Unbound | Unbound | Analogue:T5A(TMP) | |
| Reference for Active-site residues | ||
|---|---|---|
| resource | references | E.C. |
| litearture [19] & [31] | ||
| Active-site residues | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| PDB | Catalytic residues | Cofactor-binding residues | Modified residues | Main-chain involved in catalysis | Comment | |||||
| 1e2hA |
|
|
|
|
|
LYS 62;GLU 83;ARG 163;ARG 222 | THR 63;ASP 162(Magnesium binding) | |||
| 1e2hB |
|
|
|
|
|
LYS 62;GLU 83;ARG 163; | THR 63;ASP 162(Magnesium binding) | invisible 220-225 | ||
| 1e2iA |
|
|
|
|
|
LYS 62;GLU 83;ARG 163;ARG 222 | THR 63;ASP 162(Magnesium binding) | |||
| 1e2iB |
|
|
|
|
|
LYS 62;GLU 83;ARG 163; | THR 63;ASP 162(Magnesium binding) | invisible 221-224 | ||
| 1e2jA |
|
|
|
|
|
LYS 62;GLU 83;ARG 163;ARG 222 | THR 63;ASP 162(Magnesium binding) | mutant Q125N | ||
| 1e2jB |
|
|
|
|
|
LYS 62;GLU 83;ARG 163; | THR 63;ASP 162(Magnesium binding) | mutant Q125N, invisible 220-225 | ||
| 1e2kA |
|
|
|
|
|
LYS 62;GLU 83;ARG 163;ARG 222 | THR 63;ASP 162(Magnesium binding) | mutant Y101F | ||
| 1e2kB |
|
|
|
|
|
LYS 62;GLU 83;ARG 163; | THR 63;ASP 162(Magnesium binding) | mutant Y101F, invisible 221-224 | ||
| 1e2lA |
|
|
|
|
|
LYS 62;GLU 83;ARG 163;ARG 222 | THR 63;ASP 162(Magnesium binding) | mutant Y101F | ||
| 1e2lB |
|
|
|
|
|
LYS 62;GLU 83;ARG 163; | THR 63;ASP 162(Magnesium binding) | mutant Y101F, invisible 220-225 | ||
| 1e2mA |
|
|
|
|
|
LYS 62;GLU 83;ARG 163;ARG 222 | THR 63;ASP 162(Magnesium binding) | |||
| 1e2mB |
|
|
|
|
|
LYS 62;GLU 83;ARG 163; | THR 63;ASP 162(Magnesium binding) | invisible 220-225 | ||
| 1e2nA |
|
|
|
|
|
LYS 62;GLU 83;ARG 163;ARG 222 | THR 63;ASP 162(Magnesium binding) | |||
| 1e2nB |
|
|
|
|
|
LYS 62;GLU 83;ARG 163; | THR 63;ASP 162(Magnesium binding) | invisible 220-226 | ||
| 1e2pA |
|
|
|
|
|
LYS 62;GLU 83;ARG 163;ARG 222 | THR 63;ASP 162(Magnesium binding) | mutant P276S | ||
| 1e2pB |
|
|
|
|
|
LYS 62;GLU 83;ARG 163; | THR 63;ASP 162(Magnesium binding) | mutant P276S, invisible 220-225 | ||
| 1ki2A |
|
|
|
|
|
LYS 62;GLU 83;ARG 163;ARG 222 | THR 63;ASP 162(Magnesium binding) | |||
| 1ki2B |
|
|
|
|
|
LYS 62;GLU 83;ARG 163;ARG 222 | THR 63;ASP 162(Magnesium binding) | |||
| 1ki3A |
|
|
|
|
|
LYS 62;GLU 83;ARG 163;ARG 222 | THR 63;ASP 162(Magnesium binding) | |||
| 1ki3B |
|
|
|
|
|
LYS 62;GLU 83;ARG 163;ARG 222 | THR 63;ASP 162(Magnesium binding) | |||
| 1ki4A |
|
|
|
|
|
LYS 62;GLU 83;ARG 163;ARG 222 | THR 63;ASP 162(Magnesium binding) | |||
| 1ki4B |
|
|
|
|
|
LYS 62;GLU 83;ARG 163;ARG 222 | THR 63;ASP 162(Magnesium binding) | |||
| 1ki5A |
|
|
|
|
|
LYS 62;GLU 83;ARG 163;ARG 222 | THR 63;ASP 162(Magnesium binding) | |||
| 1ki5B |
|
|
|
|
|
LYS 62;GLU 83;ARG 163;ARG 222 | THR 63;ASP 162(Magnesium binding) | |||
| 1ki6A |
|
|
|
|
|
LYS 62;GLU 83;ARG 163;ARG 222 | THR 63;ASP 162(Magnesium binding) | |||
| 1ki6B |
|
|
|
|
|
LYS 62;GLU 83;ARG 163;ARG 222 | THR 63;ASP 162(Magnesium binding) | |||
| 1ki7A |
|
|
|
|
|
LYS 62;GLU 83;ARG 163;ARG 222 | THR 63;ASP 162(Magnesium binding) | |||
| 1ki7B |
|
|
|
|
|
LYS 62;GLU 83;ARG 163;ARG 222 | THR 63;ASP 162(Magnesium binding) | |||
| 1ki8A |
|
|
|
|
|
LYS 62;GLU 83;ARG 163;ARG 222 | THR 63;ASP 162(Magnesium binding) | |||
| 1ki8B |
|
|
|
|
|
LYS 62;GLU 83;ARG 163;ARG 222 | THR 63;ASP 162(Magnesium binding) | |||
| 1kimA |
|
|
|
|
|
LYS 62;GLU 83;ARG 163;ARG 222 | THR 63;ASP 162(Magnesium binding) | |||
| 1kimB |
|
|
|
|
|
LYS 62;GLU 83;ARG 163;ARG 222 | THR 63;ASP 162(Magnesium binding) | |||
| 1kinA |
|
|
|
|
|
LYS 62;GLU 83;ARG 163;ARG 222 | THR 63;ASP 162(Magnesium binding) | |||
| 1kinB |
|
|
|
|
|
LYS 62;GLU 83;ARG 163;ARG 222 | THR 63;ASP 162(Magnesium binding) | |||
| 1of1A |
|
|
|
|
|
LYS 62;GLU 83;ARG 163;ARG 222 | THR 63;ASP 162(Magnesium binding) | |||
| 1of1B |
|
|
|
|
|
LYS 62;GLU 83;ARG 163; | THR 63;ASP 162(Magnesium binding) | invisible 221-223 | ||
| 1p7cA |
|
|
|
|
|
LYS 62;GLU 83;ARG 163;ARG 222 | THR 63;ASP 162(Magnesium binding) | |||
| 1p7cB |
|
|
|
|
|
LYS 62;GLU 83;ARG 163;ARG 222 | THR 63;ASP 162(Magnesium binding) | |||
| 1qhiA |
|
|
|
|
|
LYS 62;GLU 83;ARG 163;ARG 222 | THR 63;ASP 162(Magnesium binding) | |||
| 1qhiB |
|
|
|
|
|
LYS 62;GLU 83;ARG 163;ARG 222 | THR 63;ASP 162(Magnesium binding) | |||
| 1vtkA |
|
|
|
|
|
LYS 62;GLU 83;ARG 163;ARG 222 | THR 63;ASP 162(Magnesium binding) | |||
| 2ki5A |
|
|
|
|
|
LYS 62;GLU 83;ARG 163;ARG 222 | THR 63;ASP 162(Magnesium binding) | |||
| 2ki5B |
|
|
|
|
|
LYS 62;GLU 83;ARG 163;ARG 222 | THR 63;ASP 162(Magnesium binding) | |||
| 2vtkA |
|
|
|
|
|
LYS 62;GLU 83;ARG 163;ARG 222 | THR 63;ASP 162(Magnesium binding) | |||
| 3vtkA |
|
|
|
|
|
LYS 62;GLU 83;ARG 163;ARG 222 | THR 63;ASP 162(Magnesium binding) | |||
| 1osnA |
|
|
|
|
|
LYS 25;GLU 48;ARG 130; | THR 26;ASP 129(Magnesium binding) | invisible 186-191 | ||
| 1osnB |
|
|
|
|
|
LYS 25;GLU 48;ARG 130; | THR 26;ASP 129(Magnesium binding) | invisible 186-191 | ||
| 1osnC |
|
|
|
|
|
LYS 25;GLU 48;ARG 130; | THR 26;ASP 129(Magnesium binding) | invisible 186-191 | ||
| 1osnD |
|
|
|
|
|
LYS 25;GLU 48;ARG 130; | THR 26;ASP 129(Magnesium binding) | invisible 186-191 | ||
| 1p6xA |
|
|
|
|
|
LYS 38;GLU 60;ARG 139;ARG 196 | SER 39;ASP 138(Magnesium binding) | |||
| 1p6xB |
|
|
|
|
|
LYS 38;GLU 60;ARG 139;ARG 196 | SER 39;ASP 138(Magnesium binding) | |||
| 1p72A |
|
|
|
|
|
LYS 38;GLU 60;ARG 139;ARG 196 | SER 39;ASP 138(Magnesium binding) | |||
| 1p72B |
|
|
|
|
|
LYS 38;GLU 60;ARG 139;ARG 196 | SER 39;ASP 138(Magnesium binding) | |||
| 1p73A |
|
|
|
|
|
LYS 38;GLU 60;ARG 139;ARG 196 | SER 39;ASP 138(Magnesium binding) | |||
| 1p73B |
|
|
|
|
|
LYS 38;GLU 60;ARG 139;ARG 196 | SER 39;ASP 138(Magnesium binding) | |||
| 1p73C |
|
|
|
|
|
LYS 38;GLU 60;ARG 139;ARG 196 | SER 39;ASP 138(Magnesium binding) | |||
| 1p73D |
|
|
|
|
|
LYS 38;GLU 60;ARG 139;ARG 196 | SER 39;ASP 138(Magnesium binding) | |||
| 1p75A |
|
|
|
|
|
LYS 38;GLU 60;ARG 139;ARG 196 | SER 39;ASP 138(Magnesium binding) | invisible 199 | ||
| 1p75B |
|
|
|
|
|
LYS 38;GLU 60;ARG 139;ARG 196 | SER 39;ASP 138(Magnesium binding) | |||
| 1p75C |
|
|
|
|
|
LYS 38;GLU 60;ARG 139;ARG 196 | SER 39;ASP 138(Magnesium binding) | invisible 197-199 | ||
| 1p75D |
|
|
|
|
|
LYS 38;GLU 60;ARG 139; | SER 39;ASP 138(Magnesium binding) | invisible 195-199 | ||
| References for Catalytic Mechanism | ||
|---|---|---|
| References | Sections | No. of steps in catalysis |
|
[10]
|
p.290-291 | |
|
[18]
|
p.2102-2103 | |
|
[23]
|
p.124 | |
|
[31]
|
||
|
[35]
|
||
| References | |
|---|---|
| [1] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 3003090 |
| Journal | J Biol Chem |
| Year | 1986 |
| Volume | 261 |
| Pages | 1985-7 |
| Authors | Arnold JR, Cheng MS, Cullis PM, Lowe G |
| Title | The stereochemical course of phosphoryl transfer catalyzed by herpes simplex virus type I-induced thymidine kinase. |
| Related PDB | |
| Related UniProtKB | |
| [2] | |
| Resource | |
| Comments | NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| Medline ID | |
| PubMed ID | 3007662 |
| Journal | J Gen Virol |
| Year | 1986 |
| Volume | 67 |
| Pages | 753-8 |
| Authors | Graham D, Larder BA, Inglis MM |
| Title | Evidence that the 'active centre' of the herpes simplex virus thymidine kinase involves an interaction between three distinct regions of the polypeptide. |
| Related PDB | |
| Related UniProtKB | P06479 |
| [3] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 3588286 |
| Journal | Nucleic Acids Res |
| Year | 1987 |
| Volume | 15 |
| Pages | 4111-21 |
| Authors | Parkanyi L, Kalman A, Czugler M, Kovacs T, Walker RT |
| Title | A comparison of the conformations adopted by some 5-bromovinyl-2'-deoxyuridines and a correlation with their antiviral properties: an X-ray study. |
| Related PDB | |
| Related UniProtKB | |
| [4] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8218229 |
| Journal | Biochemistry |
| Year | 1993 |
| Volume | 32 |
| Pages | 11618-26 |
| Authors | Black ME, Loeb LA |
| Title | Identification of important residues within the putative nucleoside binding site of HSV-1 thymidine kinase by random sequence selection: analysis of selected mutants in vitro. |
| Related PDB | |
| Related UniProtKB | |
| [5] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8396286 |
| Journal | Virus Genes |
| Year | 1993 |
| Volume | 7 |
| Pages | 205-9 |
| Authors | Fetzer J, Folkers G, Muller I, Keil GM |
| Title | Site-directed mutagenesis in the active site of the herpes simplex virus type 1 thymidine kinase gene. |
| Related PDB | |
| Related UniProtKB | |
| [6] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7993074 |
| Journal | Antiviral Res |
| Year | 1994 |
| Volume | 24 |
| Pages | 289-304 |
| Authors | Olivier A, Creuven I, Evrard C, Evrard G, Dory M, van Aerschot A, Wigerinck P, Herdewijn P, Durant F |
| Title | Stereoelectronic properties of five anti-HSV-1 2'-deoxynucleosides analogues with heterocyclic substituents in the 5-position: a comparison with BVDU. |
| Related PDB | |
| Related UniProtKB | |
| [7] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8068016 |
| Journal | Biochem J |
| Year | 1994 |
| Volume | 302 |
| Pages | 279-82 |
| Authors | Maga G, Focher F, Wright GE, Capobianco M, Garbesi A, Bendiscioli A, Spadari S |
| Title | Kinetic studies with N2-phenylguanines and with L-thymidine indicate that herpes simplex virus type-1 thymidine kinase and thymidylate kinase share a common active site. |
| Related PDB | |
| Related UniProtKB | |
| [8] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7957251 |
| Journal | Eur J Biochem |
| Year | 1994 |
| Volume | 226 |
| Pages | 219-26 |
| Authors | Michael M, Fetzer J, Folkers G |
| Title | Site-directed mutagenesis clarifies the substrate position within the three-dimensional model of the active site of herpes simplex virus type-1 thymidine kinase. |
| Related PDB | |
| Related UniProtKB | |
| [9] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7733991 |
| Journal | Biochem Biophys Res Commun |
| Year | 1995 |
| Volume | 209 |
| Pages | 966-73 |
| Authors | Michael M, Fetzer J, Folkers G |
| Title |
Site-directed mutagenesis of herpes simplex virus type 1 thymidine kinase opposes the importance of amino acid positions 251, |
| Related PDB | |
| Related UniProtKB | |
| [10] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 34-376. |
| Medline ID | |
| PubMed ID | 7628623 |
| Journal | FEBS Lett |
| Year | 1995 |
| Volume | 368 |
| Pages | 289-92 |
| Authors | Wild K, Bohner T, Aubry A, Folkers G, Schulz GE |
| Title | The three-dimensional structure of thymidine kinase from herpes simplex virus type 1. |
| Related PDB | |
| Related UniProtKB | P03176 |
| [11] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS). |
| Medline ID | |
| PubMed ID | 7552712 |
| Journal | Nat Struct Biol |
| Year | 1995 |
| Volume | 2 |
| Pages | 876-81 |
| Authors | Brown DG, Visse R, Sandhu G, Davies A, Rizkallah PJ, Melitz C, Summers WC, Sanderson MR |
| Title | Crystal structures of the thymidine kinase from herpes simplex virus type-1 in complex with deoxythymidine and ganciclovir. |
| Related PDB | 1kin |
| Related UniProtKB | P03176 |
| [12] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8660548 |
| Journal | Anal Biochem |
| Year | 1996 |
| Volume | 237 |
| Pages | 135-40 |
| Authors | Rechtin TM, Black ME, Drake RR |
| Title | Proteolytic mapping of the thymidine/thymidylate binding site of herpes simplex virus type 1 thymidine kinase: a general photoaffinity labeling method for identifying active-site peptides. |
| Related PDB | |
| Related UniProtKB | |
| [13] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8783799 |
| Journal | Antiviral Res |
| Year | 1996 |
| Volume | 30 |
| Pages | 63-74 |
| Authors | Creuven I, Evrard C, Olivier A, Evrard G, Van Aerschot A, Wigerinck P, Herdewijn P, Durant F |
| Title | Relationship between structural properties and affinity for herpes simplex virus type 1 thymidine kinase of bromine substituted 5-heteroaromatic 2'-deoxyuridines. |
| Related PDB | |
| Related UniProtKB | |
| [14] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8941385 |
| Journal | J Med Chem |
| Year | 1996 |
| Volume | 39 |
| Pages | 4727-37 |
| Authors | De Winter H, Herdewijn P |
| Title | Understanding the binding of 5-substituted 2'-deoxyuridine substrates to thymidine kinase of herpes simplex virus type-1. |
| Related PDB | |
| Related UniProtKB | |
| [15] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8622970 |
| Journal | Proc Natl Acad Sci U S A |
| Year | 1996 |
| Volume | 93 |
| Pages | 3525-9 |
| Authors | Black ME, Newcomb TG, Wilson HM, Loeb LA |
| Title | Creation of drug-specific herpes simplex virus type 1 thymidine kinase mutants for gene therapy. |
| Related PDB | |
| Related UniProtKB | |
| [16] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9450049 |
| Journal | Biochem Soc Trans |
| Year | 1997 |
| Volume | 25 |
| Pages | S621 |
| Authors | Evans JS, Lock KP, Levine BA, Champness JN, Sanderson MR, Summers WC, Buchan A |
| Title | Structure-specificity features of HSV-1 thymidine kinases. |
| Related PDB | |
| Related UniProtKB | |
| [17] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9029509 |
| Journal | J Recept Signal Transduct Res |
| Year | 1997 |
| Volume | 17 |
| Pages | 475-94 |
| Authors | Folkers G, Alber F, Amrhein I, Behrends H, Bohner T, Gerber S, Kuonen O, Scapozza L |
| Title | Integrated homology modelling and X-ray study of herpes simplex virus I thymidine kinase: a case study. |
| Related PDB | |
| Related UniProtKB | |
| [18] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.75 ANGSTROMS) OF 34-376. |
| Medline ID | |
| PubMed ID | 9336833 |
| Journal | Protein Sci |
| Year | 1997 |
| Volume | 6 |
| Pages | 2097-106 |
| Authors | Wild K, Bohner T, Folkers G, Schulz GE |
| Title | The structures of thymidine kinase from herpes simplex virus type 1 in complex with substrates and a substrate analogue. |
| Related PDB | 1vtk 2vtk 3vtk |
| Related UniProtKB | P03176 |
| [19] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9716390 |
| Journal | Eur J Biochem |
| Year | 1998 |
| Volume | 255 |
| Pages | 472-81 |
| Authors | Kussmann-Gerber S, Kuonen O, Folkers G, Pilger BD, Scapozza L |
| Title | Drug resistance of herpes simplex virus type 1--structural considerations at the molecular level of the thymidine kinase. |
| Related PDB | |
| Related UniProtKB | |
| [20] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9747715 |
| Journal | J Gen Virol |
| Year | 1998 |
| Volume | 79 |
| Pages | 2083-92 |
| Authors | Evans JS, Lock KP, Levine BA, Champness JN, Sanderson MR, Summers WC, McLeish PJ, Buchan A |
| Title | Herpesviral thymidine kinases: laxity and resistance by design. |
| Related PDB | |
| Related UniProtKB | |
| [21] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS). |
| Medline ID | |
| PubMed ID | 9715911 |
| Journal | Proteins |
| Year | 1998 |
| Volume | 32 |
| Pages | 350-61 |
| Authors | Champness JN, Bennett MS, Wien F, Visse R, Summers WC, Herdewijn P, de Clerq E, Ostrowski T, Jarvest RL, Sanderson MR |
| Title | Exploring the active site of herpes simplex virus type-1 thymidine kinase by X-ray crystallography of complexes with aciclovir and other ligands. |
| Related PDB | 1ki2 1ki3 1ki4 1ki5 1ki6 1ki7 1ki8 1kim |
| Related UniProtKB | P03176 |
| [22] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10386936 |
| Journal | Bioorg Med Chem Lett |
| Year | 1999 |
| Volume | 9 |
| Pages | 1563-6 |
| Authors | Wouters J, Herdewijn P |
| Title | 5-Substituted pyrimidine 1,5-anhydrohexitols: conformational analysis and interaction with viral thymidine kinase. |
| Related PDB | |
| Related UniProtKB | |
| [23] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS). |
| Medline ID | |
| PubMed ID | 9989588 |
| Journal | FEBS Lett |
| Year | 1999 |
| Volume | 443 |
| Pages | 121-5 |
| Authors | Bennett MS, Wien F, Champness JN, Batuwangala T, Rutherford T, Summers WC, Sun H, Wright G, Sanderson MR |
| Title |
Structure to 1.9 A resolution of a complex with herpes simplex virus type-1 thymidine kinase of a novel, |
| Related PDB | 1qhi 2ki5 |
| Related UniProtKB | P03176 |
| [24] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10542226 |
| Journal | J Biol Chem |
| Year | 1999 |
| Volume | 274 |
| Pages | 31967-73 |
| Authors | Pilger BD, Perozzo R, Alber F, Wurth C, Folkers G, Scapozza L |
| Title |
Substrate diversity of herpes simplex virus thymidine kinase. |
| Related PDB | |
| Related UniProtKB | |
| [25] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10358938 |
| Journal | Nucleosides Nucleotides |
| Year | 1999 |
| Volume | 18 |
| Pages | 311-30 |
| Authors | Kussmann-Gerber S, Wurth C, Scapozza L, Pilger BD, Pliska V, Folkers G |
| Title | Interaction of the recombinant herpes simplex virus type 1 thymidine kinase with thymidine and aciclovir: a kinetic study. |
| Related PDB | |
| Related UniProtKB | |
| [26] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10747801 |
| Journal | Biochemistry |
| Year | 2000 |
| Volume | 39 |
| Pages | 4105-11 |
| Authors | Hinds TA, Compadre C, Hurlburt BK, Drake RR |
| Title | Conservative mutations of glutamine-125 in herpes simplex virus type 1 thymidine kinase result in a ganciclovir kinase with minimal deoxypyrimidine kinase activities. |
| Related PDB | |
| Related UniProtKB | |
| [27] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 10924157 |
| Journal | Biochemistry |
| Year | 2000 |
| Volume | 39 |
| Pages | 9597-603 |
| Authors | Prota A, Vogt J, Pilger B, Perozzo R, Wurth C, Marquez VE, Russ P, Schulz GE, Folkers G, Scapozza L |
| Title | Kinetics and crystal structure of the wild-type and the engineered Y101F mutant of Herpes simplex virus type 1 thymidine kinase interacting with (North)-methanocarba-thymidine. |
| Related PDB | 1e2k 1e2l |
| Related UniProtKB | |
| [28] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10747922 |
| Journal | J Biol Chem |
| Year | 2000 |
| Volume | 275 |
| Pages | 16139-45 |
| Authors | Perozzo R, Jelesarov I, Bosshard HR, Folkers G, Scapozza L |
| Title |
Compulsory order of substrate binding to herpes simplex virus type 1 thymidine kinase. |
| Related PDB | |
| Related UniProtKB | |
| [29] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10935205 |
| Journal | J Mol Graph Model |
| Year | 2000 |
| Volume | 18 |
| Pages | 33-41 |
| Authors | Gaudio AC, Richards WG, Takahata Y |
| Title | QSAR and molecular graphics analysis of N2-phenylguanines as inhibitors of herpes simplex virus thymidine kinases. |
| Related PDB | |
| Related UniProtKB | |
| [30] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 11056041 |
| Journal | Proteins |
| Year | 2000 |
| Volume | 41 |
| Pages | 545-53 |
| Authors | Vogt J, Perozzo R, Pautsch A, Prota A, Schelling P, Pilger B, Folkers G, Scapozza L, Schulz GE |
| Title | Nucleoside binding site of herpes simplex type 1 thymidine kinase analyzed by X-ray crystallography. |
| Related PDB | 1e2h 1e2i 1e2j |
| Related UniProtKB | |
| [31] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11262392 |
| Journal | J Biol Chem |
| Year | 2001 |
| Volume | 276 |
| Pages | 21692-7 |
| Authors | Sulpizi M, Schelling P, Folkers G, Carloni P, Scapozza L |
| Title |
The rational of catalytic activity of herpes simplex virus thymidine kinase. |
| Related PDB | |
| Related UniProtKB | |
| [32] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11676546 |
| Journal | J Mol Biol |
| Year | 2001 |
| Volume | 313 |
| Pages | 657-70 |
| Authors | Wurth C, Thomas RM, Folkers G, Scapozza L |
| Title | Folding and self-assembly of herpes simplex virus type 1 thymidine kinase. |
| Related PDB | |
| Related UniProtKB | |
| [33] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 11266595 |
| Journal | Protein Sci |
| Year | 2001 |
| Volume | 10 |
| Pages | 63-73 |
| Authors | Wurth C, Kessler U, Vogt J, Schulz GE, Folkers G, Scapozza L |
| Title | The effect of substrate binding on the conformation and structural stability of Herpes simplex virus type 1 thymidine kinase. |
| Related PDB | 1e2m 1e2n 1e2p |
| Related UniProtKB | |
| [34] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 12686543 |
| Journal | J Biol Chem |
| Year | 2003 |
| Volume | 278 |
| Pages | 24680-7 |
| Authors | Bird LE, Ren J, Wright A, Leslie KD, Degreve B, Balzarini J, Stammers DK |
| Title | Crystal structure of varicella zoster virus thymidine kinase. |
| Related PDB | 1osn |
| Related UniProtKB | |
| [35] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 14527394 |
| Journal | Structure (Camb) |
| Year | 2003 |
| Volume | 11 |
| Pages | 1265-77 |
| Authors | Gardberg A, Shuvalova L, Monnerjahn C, Konrad M, Lavie A |
| Title | Structural basis for the dual thymidine and thymidylate kinase activity of herpes thymidine kinases. |
| Related PDB | 1p7c 1p6x 1p72 1p73 1p75 |
| Related UniProtKB | |
| [36] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 14705959 |
| Journal | Biochem J |
| Year | 2004 |
| Volume | 379 |
| Pages | 795-803 |
| Authors | Wu CC, Chen MC, Chang YR, Hsu TY, Chen JY |
| Title | Identification and characterization of the conserved nucleoside-binding sites in the Epstein-Barr virus thymidine kinase. |
| Related PDB | |
| Related UniProtKB | |
| [37] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 14719951 |
| Journal | J Am Chem Soc |
| Year | 2004 |
| Volume | 126 |
| Pages | 543-9 |
| Authors | Marquez VE, Ben-Kasus T, Barchi JJ Jr, Green KM, Nicklaus MC, Agbaria R |
| Title | Experimental and structural evidence that herpes 1 kinase and cellular DNA polymerase(s) discriminate on the basis of sugar pucker. |
| Related PDB | |
| Related UniProtKB | |
| [38] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 15163659 |
| Journal | J Biol Chem |
| Year | 2004 |
| Volume | 279 |
| Pages | 32832-8 |
| Authors | Schelling P, Claus MT, Johner R, Marquez VE, Schulz GE, Scapozza L |
| Title | Biochemical and structural characterization of (South)-methanocarbathymidine that specifically inhibits growth of herpes simplex virus type 1 thymidine kinase-transduced osteosarcoma cells. |
| Related PDB | 1of1 |
| Related UniProtKB | |
| Comments |
|---|
|
Although there has been no structure with magnesium ion bound to the active site, The catalytic mechanism proceeds as follows: (1) Glu83 acts as a general base to activate the acceptor group, (2) The activated acceptor group makes a nucleophilic attack on the transferred group, (3) During the transition state, |
| Created | Updated |
|---|---|
| 2002-05-31 | 2009-02-26 |