DB code: M00104
| RLCP classification | 3.103.90020.1136 : Transfer | |
|---|---|---|
| CATH domain | 1.10.1320.10 : T7 RNA polymerase; domain 1 | |
| 1.10.287.260 : Helix Hairpins | ||
| 3.30.70.370 : Alpha-Beta Plaits | Catalytic domain | |
| 1.10.287.280 : Helix Hairpins | ||
| 1.10.150.20 : DNA polymerase; domain 1 | Catalytic domain | |
| E.C. | 2.7.7.6 | |
| CSA | 1s76 | |
| M-CSA | 1s76 | |
| MACiE | ||
| CATH domain | Related DB codes (homologues) |
|---|---|
| 1.10.150.20 : DNA polymerase; domain 1 | M00055 M00173 M00175 M00208 D00158 |
| 1.10.287.280 : Helix Hairpins | M00019 |
| 3.30.70.370 : Alpha-Beta Plaits | M00055 M00173 M00175 |
| Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | RefSeq | Pfam |
|---|---|---|---|---|
| P00573 |
DNA-directed RNA polymerase
|
EC
2.7.7.6
|
NP_041960.1
(Protein)
NC_001604.1 (DNA/RNA sequence) |
PF00940
(RNA_pol)
[Graphical View] |
| KEGG enzyme name |
|---|
|
C RNA formation factors
DNA-directed RNA polymerase RNA polymerase RNA nucleotidyltransferase (DNA-directed) RNA polymerase I RNA polymerase II RNA polymerase III RNA nucleotidyltransferase (DNA-directed) deoxyribonucleic acid-dependent ribonucleic acid polymerase DNA-dependent ribonucleate nucleotidyltransferase DNA-dependent RNA nucleotidyltransferase DNA-dependent RNA polymerase ribonucleate nucleotidyltransferase ribonucleate polymerase C ribonucleic acid formation factors ribonucleic acid nucleotidyltransferase ribonucleic acid polymerase ribonucleic acid transcriptase ribonucleic polymerase ribonucleic transcriptase C RNA formation factors RNA nucleotidyltransferase RNA transcriptase transcriptase |
| UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
|---|---|---|---|---|---|
| P00573 | RPOL_BPT7 | Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). | Monomer. |
| KEGG Pathways | Map code | Pathways | E.C. |
|---|---|---|
| MAP00230 | Purine metabolism | |
| MAP00240 | Pyrimidine metabolism |
| Compound table | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Cofactors | Substrates | Products | Intermediates | ||||||||
| KEGG-id | C00305 | C00201 | C00046 | C00013 | C00046 | ||||||
| E.C. | |||||||||||
| Compound | Magnesium | Nucleoside triphosphate | RNA(n) | Pyrophosphate | RNA(n+1) | ||||||
| Type | divalent metal (Ca2+, Mg2+) | nucleotide | nucleic acids | phosphate group/phosphate ion | nucleic acids | ||||||
| ChEBI |
18420 18420 |
29888 29888 |
|||||||||
| PubChem |
888 888 |
1023 21961011 1023 21961011 |
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| 1aroP01 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1cezA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1h38A01 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1h38B01 |
|
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|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1h38C01 |
|
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|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1h38D01 |
|
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|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1mswD01 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1qlnA01 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1s0vA01 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1s0vB01 |
|
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|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1s0vC01 |
|
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|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1s0vD01 |
|
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|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1s76D01 |
|
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|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1s77D01 |
|
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|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1aroP02 |
|
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|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1cezA02 |
|
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|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1h38A02 |
|
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|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1h38B02 |
|
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|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1h38C02 |
|
|
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|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1h38D02 |
|
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|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1mswD02 |
|
|
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|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1qlnA02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1s0vA02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1s0vB02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1s0vC02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1s0vD02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1s76D02 |
|
|
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|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1s77D02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1aroP03 |
|
|
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|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1cezA03 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1h38A03 |
|
|
|
|
|
Unbound | Unbound | Bound:G-C-G-G-C-G-A-U (chain F) | Unbound | Unbound | |
| 1h38B03 |
|
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|
Unbound | Unbound | Bound:G-C-G-G-C-G-A-U (chain I) | Unbound | Unbound | |
| 1h38C03 |
|
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|
Unbound | Unbound | Bound:G-C-G-G-C-G-A-U (chain L) | Unbound | Unbound | |
| 1h38D03 |
|
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|
Unbound | Unbound | Bound:G-C-G-G-C-G-A-U (chain O) | Unbound | Unbound | |
| 1mswD03 |
|
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|
|
Unbound | Unbound | Bound:G-A-C-A-C-G-G-C-G-A (chain R) | Unbound | Unbound | |
| 1qlnA03 |
|
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|
Unbound | Unbound | Bound:GTP-G-G (chain R) | Unbound | Unbound | |
| 1s0vA03 |
|
|
|
|
|
Bound:2x_MG | Unbound | Bound:G-C-G-G-C-G-A-U (chain F) | Unbound | Unbound | |
| 1s0vB03 |
|
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|
Bound:2x_MG | Unbound | Bound:G-C-G-G-C-G-A-U (chain I) | Unbound | Unbound | |
| 1s0vC03 |
|
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|
|
Bound:2x_MG | Unbound | Bound:G-C-G-G-C-G-A-U (chain L) | Unbound | Unbound | |
| 1s0vD03 |
|
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|
Bound:2x_MG | Unbound | Bound:G-C-G-G-C-G-A-U (chain O) | Unbound | Unbound | |
| 1s76D03 |
|
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Bound:2x_MG | Unbound | Bound:A-C-A-C-G-G-C-G-A (chain R) | Unbound | Unbound | |
| 1s77D03 |
|
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Bound:2x_MG | Unbound | Unbound | Unbound | Bound:A-C-A-C-G-G-C-G-A-3DA (chain R) | |
| 1aroP04 |
|
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|
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1cezA04 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1h38A04 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1h38B04 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1h38C04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1h38D04 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1mswD04 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1qlnA04 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1s0vA04 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1s0vB04 |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1s0vC04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1s0vD04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1s76D04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1s77D04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1aroP05 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1cezA05 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1h38A05 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1h38B05 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1h38C05 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1h38D05 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1mswD05 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1qlnA05 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1s0vA05 |
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Unbound | Analogue:APC | Unbound | Unbound | Unbound | |
| 1s0vB05 |
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Unbound | Analogue:APC | Unbound | Unbound | Unbound | |
| 1s0vC05 |
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Unbound | Analogue:APC | Unbound | Unbound | Unbound | |
| 1s0vD05 |
|
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Unbound | Analogue:APC | Unbound | Unbound | Unbound | |
| 1s76D05 |
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Unbound | Analogue:APC | Unbound | Unbound | Unbound | |
| 1s77D05 |
|
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Unbound | Unbound | Unbound | Bound:POP | Unbound | |
| Reference for Active-site residues | ||
|---|---|---|
| resource | references | E.C. |
| swiss-prot:P00573 | ||
| Active-site residues | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| PDB | Catalytic residues | Cofactor-binding residues | Modified residues | Main-chain involved in catalysis | Comment | |||||
| 1aroP01 |
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| 1cezA01 |
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| 1h38A01 |
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| 1h38B01 |
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| 1h38C01 |
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| 1h38D01 |
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| 1mswD01 |
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| 1qlnA01 |
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| 1s0vA01 |
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| 1s0vB01 |
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| 1s0vC01 |
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| 1s0vD01 |
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| 1s76D01 |
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| 1s77D01 |
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| 1aroP02 |
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| 1cezA02 |
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| 1h38A02 |
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| 1h38B02 |
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| 1h38C02 |
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| 1h38D02 |
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| 1mswD02 |
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| 1qlnA02 |
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| 1s0vA02 |
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| 1s0vB02 |
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| 1s0vC02 |
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| 1s0vD02 |
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| 1s76D02 |
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| 1s77D02 |
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| 1aroP03 |
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ASP 537;GLY 538;ASP 812(Two magnesium ions) | ||||
| 1cezA03 |
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ASP 537;GLY 538;ASP 812(Two magnesium ions) | ||||
| 1h38A03 |
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ASP 537;GLY 538;ASP 812(Two magnesium ions) | ||||
| 1h38B03 |
|
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ASP 537;GLY 538;ASP 812(Two magnesium ions) | ||||
| 1h38C03 |
|
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ASP 537;GLY 538;ASP 812(Two magnesium ions) | ||||
| 1h38D03 |
|
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ASP 537;GLY 538;ASP 812(Two magnesium ions) | ||||
| 1mswD03 |
|
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|
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ASP 537;GLY 538;ASP 812(Two magnesium ions) | ||||
| 1qlnA03 |
|
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ASP 537;GLY 538;ASP 812(Two magnesium ions) | ||||
| 1s0vA03 |
|
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ASP 537;GLY 538;ASP 812(Two magnesium ions) | ||||
| 1s0vB03 |
|
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ASP 537;GLY 538;ASP 812(Two magnesium ions) | ||||
| 1s0vC03 |
|
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ASP 537;GLY 538;ASP 812(Two magnesium ions) | ||||
| 1s0vD03 |
|
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ASP 537;GLY 538;ASP 812(Two magnesium ions) | ||||
| 1s76D03 |
|
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ASP 537;GLY 538;ASP 812(Two magnesium ions) | ||||
| 1s77D03 |
|
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ASP 537;GLY 538;ASP 812(Two magnesium ions) | ||||
| 1aroP04 |
|
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| 1cezA04 |
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| 1h38A04 |
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| 1h38B04 |
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| 1h38C04 |
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| 1h38D04 |
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| 1mswD04 |
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| 1qlnA04 |
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| 1s0vA04 |
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| 1s0vB04 |
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| 1s0vC04 |
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| 1s0vD04 |
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| 1s76D04 |
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| 1s77D04 |
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| 1aroP05 |
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ARG 627;LYS 631 | ||||
| 1cezA05 |
|
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ARG 627;LYS 631 | ||||
| 1h38A05 |
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ARG 627;LYS 631 | ||||
| 1h38B05 |
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ARG 627;LYS 631 | ||||
| 1h38C05 |
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ARG 627;LYS 631 | ||||
| 1h38D05 |
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ARG 627;LYS 631 | ||||
| 1mswD05 |
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ARG 627;LYS 631 | ||||
| 1qlnA05 |
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ARG 627;LYS 631 | ||||
| 1s0vA05 |
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ARG 627;LYS 631 | ||||
| 1s0vB05 |
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ARG 627;LYS 631 | ||||
| 1s0vC05 |
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ARG 627;LYS 631 | ||||
| 1s0vD05 |
|
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ARG 627;LYS 631 | ||||
| 1s76D05 |
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ARG 627;LYS 631 | ||||
| 1s77D05 |
|
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|
ARG 627;LYS 631 | ||||
| References for Catalytic Mechanism | ||
|---|---|---|
| References | Sections | No. of steps in catalysis |
|
[7]
|
||
|
[16]
|
p.266-267 | |
|
[17]
|
Fig.1, p.705-707 | |
|
[32]
|
Fig.3 | |
| References | |
|---|---|
| [1] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1847871 |
| Journal | Eur J Biochem |
| Year | 1991 |
| Volume | 195(3) |
| Pages | 841-7 |
| Authors | Maksimova TG, Mustayev AA, Zaychikov EF, Lyakhov DL, Tunitskaya VL, Akbarov AKh, Luchin SV, Rechinsky VO, Chernov BK, Kochetkov SN |
| Title |
Lys631 residue in the active site of the bacteriophage T7 RNA polymerase. |
| Related PDB | |
| Related UniProtKB | P00573 |
| [2] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8462683 |
| Journal | FEBS Lett |
| Year | 1993 |
| Volume | 320(1) |
| Pages | 9-12 |
| Authors | Rechinsky VO, Tunitskaya VL, Dragan SM, Kostyuk DA, Kochetkov SN |
| Title | Tyr-571 is involved in the T7 RNA polymerase binding to its promoter. |
| Related PDB | |
| Related UniProtKB | P00573 |
| [3] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8492813 |
| Journal | Mol Gen Genet |
| Year | 1993 |
| Volume | 238(3) |
| Pages | 455-8 |
| Authors | Rechinsky VO, Kostyuk DA, Lyakhov DL, Chernov BK, Kochetkov SN |
| Title | Random mutagenesis of the gene for bacteriophage T7 RNA polymerase. |
| Related PDB | |
| Related UniProtKB | P00573 |
| [4] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8475053 |
| Journal | Proc Natl Acad Sci U S A |
| Year | 1993 |
| Volume | 90(8) |
| Pages | 3147-51 |
| Authors | Raskin CA, Diaz GA, McAllister WT |
| Title | T7 RNA polymerase mutants with altered promoter specificities. |
| Related PDB | |
| Related UniProtKB | |
| [5] | |
| Resource | |
| Comments |
Comments on: Nature. |
| Medline ID | |
| PubMed ID | 7688863 |
| Journal | Nature |
| Year | 1993 |
| Volume | 364(6438) |
| Pages | 572-3 |
| Authors | Moras D |
| Title |
Polymerases. |
| Related PDB | |
| Related UniProtKB | |
| [6] | |
| Resource | |
| Comments |
Comments in: Nature. |
| Medline ID | |
| PubMed ID | 7688864 |
| Journal | Nature |
| Year | 1993 |
| Volume | 364(6438) |
| Pages | 593-9 |
| Authors | Sousa R, Chung YJ, Rose JP, Wang BC |
| Title | Crystal structure of bacteriophage T7 RNA polymerase at 3.3 A resolution. |
| Related PDB | 4rnp |
| Related UniProtKB | P00573 |
| [7] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8133519 |
| Journal | J Mol Biol |
| Year | 1994 |
| Volume | 237(1) |
| Pages | 5-19 |
| Authors | Osumi-Davis PA, Sreerama N, Volkin DB, Middaugh CR, Woody RW, Woody AY |
| Title |
Bacteriophage T7 RNA polymerase and its active-site mutants. |
| Related PDB | |
| Related UniProtKB | P00573 |
| [8] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8171031 |
| Journal | Proc Natl Acad Sci U S A |
| Year | 1994 |
| Volume | 91(9) |
| Pages | 4034-8 |
| Authors | Cheng X, Zhang X, Pflugrath JW, Studier FW |
| Title |
The structure of bacteriophage T7 lysozyme, |
| Related PDB | |
| Related UniProtKB | P00806 |
| [9] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7966322 |
| Journal | J Mol Biol |
| Year | 1994 |
| Volume | 244(1) |
| Pages | 6-12 |
| Authors | Sousa R, Rose J, Wang BC |
| Title |
The thumb's knuckle. |
| Related PDB | |
| Related UniProtKB | |
| [10] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8885831 |
| Journal | Biochemistry |
| Year | 1996 |
| Volume | 35(42) |
| Pages | 13519-30 |
| Authors | Sastry SS |
| Title | Identification of the template-binding cleft of T7 RNA polymerase as the site for promoter binding by photochemical cross-linking with psoralen. |
| Related PDB | |
| Related UniProtKB | |
| [11] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9062120 |
| Journal | Biochemistry |
| Year | 1997 |
| Volume | 36(10) |
| Pages | 2908-18 |
| Authors | Gardner LP, Mookhtiar KA, Coleman JE |
| Title |
Initiation, |
| Related PDB | |
| Related UniProtKB | |
| [12] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9405156 |
| Journal | J Mol Biol |
| Year | 1997 |
| Volume | 274(5) |
| Pages | 748-56 |
| Authors | Jeruzalmi D, Steitz TA |
| Title | Use of organic cosmotropic solutes to crystallize flexible proteins: application to T7 RNA polymerase and its complex with the inhibitor T7 lysozyme. |
| Related PDB | |
| Related UniProtKB | |
| [13] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9603929 |
| Journal | J Biol Chem |
| Year | 1998 |
| Volume | 273(23) |
| Pages | 14242-6 |
| Authors | Izawa M, Sasaki N, Watahiki M, Ohara E, Yoneda Y, Muramatsu M, Okazaki Y, Hayashizaki Y |
| Title | Recognition sites of 3'-OH group by T7 RNA polymerase and its application to transcriptional sequencing. |
| Related PDB | |
| Related UniProtKB | |
| [14] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9670025 |
| Journal | EMBO J |
| Year | 1998 |
| Volume | 17(14) |
| Pages | 4101-13 |
| Authors | Jeruzalmi D, Steitz TA |
| Title | Structure of T7 RNA polymerase complexed to the transcriptional inhibitor T7 lysozyme. |
| Related PDB | 1aro |
| Related UniProtKB | P00573 P00806 |
| [15] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9689042 |
| Journal | Proc Natl Acad Sci U S A |
| Year | 1998 |
| Volume | 95(16) |
| Pages | 9111-6 |
| Authors | Sastry S, Ross BM |
| Title | RNA-binding site in T7 RNA polymerase. |
| Related PDB | |
| Related UniProtKB | |
| [16] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9872383 |
| Journal | FEBS Lett |
| Year | 1998 |
| Volume | 440(3) |
| Pages | 264-7 |
| Authors | Kochetkov SN, Rusakova EE, Tunitskaya VL |
| Title | Recent studies of T7 RNA polymerase mechanism. |
| Related PDB | |
| Related UniProtKB | |
| [17] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9914251 |
| Journal | Curr Opin Struct Biol |
| Year | 1998 |
| Volume | 8 |
| Pages | 704-12 |
| Authors | Doublie S, Ellenberger T |
| Title | The mechanism of action of T7 DNA polymerase. |
| Related PDB | |
| Related UniProtKB | |
| [18] | |
| Resource | |
| Comments | Erratum in Nature 1999;400(6739):89. |
| Medline ID | |
| PubMed ID | 10331394 |
| Journal | Nature |
| Year | 1999 |
| Volume | 399(6731) |
| Pages | 80-3 |
| Authors | Cheetham GM, Jeruzalmi D, Steitz TA |
| Title | Structural basis for initiation of transcription from an RNA polymerase-promoter complex. |
| Related PDB | 1cez |
| Related UniProtKB | |
| [19] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10213599 |
| Journal | Biochemistry |
| Year | 1999 |
| Volume | 38(16) |
| Pages | 4972-81 |
| Authors | Sastry S, Ross BM |
| Title | Probing the interaction of T7 RNA polymerase with promoter. |
| Related PDB | |
| Related UniProtKB | |
| [20] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10600732 |
| Journal | Science |
| Year | 1999 |
| Volume | 286(5448) |
| Pages | 2305-9 |
| Authors | Cheetham GM, Steitz TA |
| Title | Structure of a transcribing T7 RNA polymerase initiation complex. |
| Related PDB | 1qln |
| Related UniProtKB | |
| [21] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10653695 |
| Journal | J Mol Biol |
| Year | 2000 |
| Volume | 295(5) |
| Pages | 1173-84 |
| Authors | Ujvari A, Martin CT |
| Title | Evidence for DNA bending at the T7 RNA polymerase promoter. |
| Related PDB | |
| Related UniProtKB | |
| [22] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10653635 |
| Journal | Biochemistry |
| Year | 2000 |
| Volume | 39(5) |
| Pages | 919-23 |
| Authors | Brieba LG, Sousa R |
| Title | Roles of histidine 784 and tyrosine 639 in ribose discrimination by T7 RNA polymerase. |
| Related PDB | |
| Related UniProtKB | |
| [23] | |
| Resource | |
| Comments |
Comments in: Curr Opin Struct Biol. |
| Medline ID | |
| PubMed ID | 10679468 |
| Journal | Curr Opin Struct Biol |
| Year | 2000 |
| Volume | 10(1) |
| Pages | 117-23 |
| Authors | Cheetham GM, Steitz TA |
| Title | Insights into transcription: structure and function of single-subunit DNA-dependent RNA polymerases. |
| Related PDB | |
| Related UniProtKB | |
| [24] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10995224 |
| Journal | Biochemistry |
| Year | 2000 |
| Volume | 39(38) |
| Pages | 11571-80 |
| Authors | Huang J, Brieba LG, Sousa R |
| Title | Misincorporation by wild-type and mutant T7 RNA polymerases: identification of interactions that reduce misincorporation rates by stabilizing the catalytically incompetent open conformation. |
| Related PDB | |
| Related UniProtKB | |
| [25] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11095736 |
| Journal | Proc Natl Acad Sci U S A |
| Year | 2000 |
| Volume | 97(26) |
| Pages | 14109-14 |
| Authors | Temiakov D, Mentesana PE, Ma K, Mustaev A, Borukhov S, McAllister WT |
| Title |
The specificity loop of T7 RNA polymerase interacts first with the promoter and then with the elongating transcript, |
| Related PDB | |
| Related UniProtKB | |
| [26] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11300767 |
| Journal | Biochemistry |
| Year | 2001 |
| Volume | 40(13) |
| Pages | 3882-90 |
| Authors | Brieba LG, Sousa R |
| Title | The T7 RNA polymerase intercalating hairpin is important for promoter opening during initiation but not for RNA displacement or transcription bubble stability during elongation. |
| Related PDB | |
| Related UniProtKB | |
| [27] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11278877 |
| Journal | J Biol Chem |
| Year | 2001 |
| Volume | 276(17) |
| Pages | 14075-82 |
| Authors | Bandwar RP, Patel SS |
| Title | Peculiar 2-aminopurine fluorescence monitors the dynamics of open complex formation by bacteriophage T7 RNA polymerase. |
| Related PDB | |
| Related UniProtKB | |
| [28] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11704669 |
| Journal | J Biol Chem |
| Year | 2002 |
| Volume | 277(4) |
| Pages | 2987-91 |
| Authors | McGinness KE, Joyce GF |
| Title | Substitution of ribonucleotides in the T7 RNA polymerase promoter element |
| Related PDB | |
| Related UniProtKB | |
| [29] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12422209 |
| Journal | Nature |
| Year | 2002 |
| Volume | 420 |
| Pages | 43-50 |
| Authors | Tahirov TH, Temiakov D, Anikin M, Patlan V, McAllister WT, Vassylyev DG, Yokoyama S |
| Title | Structure of a T7 RNA polymerase elongation complex at 2.9 A resolution. |
| Related PDB | 1h38 |
| Related UniProtKB | |
| [30] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12242451 |
| Journal | Science |
| Year | 2002 |
| Volume | 298 |
| Pages | 1387-95 |
| Authors | Yin YW, Steitz TA |
| Title | Structural basis for the transition from initiation to elongation transcription in T7 RNA polymerase. |
| Related PDB | 1msw |
| Related UniProtKB | |
| [31] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12499566 |
| Journal | Acta Crystallogr D Biol Crystallogr |
| Year | 2003 |
| Volume | 59 |
| Pages | 185-7 |
| Authors | Temiakov D, Tahirov TH, Anikin M, McAllister WT, Vassylyev DG, Yokoyama S |
| Title | Crystallization and preliminary crystallographic analysis of T7 RNA polymerase elongation complex. |
| Related PDB | 1s0v |
| Related UniProtKB | |
| [32] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 15016374 |
| Journal | Cell |
| Year | 2004 |
| Volume | 116 |
| Pages | 393-404 |
| Authors | Yin YW, Steitz TA |
| Title | The structural mechanism of translocation and helicase activity in T7 RNA polymerase. |
| Related PDB | 1s76 1s77 |
| Related UniProtKB | |
| Comments |
|---|
|
According to the literature [17] & [32], (1) Magnesium-A, (2) The activated acceptor group, (3) The transition state is stabilized by the following way. ## This nucleophilic substitution must be associative reaction, |
| Created | Updated |
|---|---|
| 2002-08-30 | 2009-02-26 |