DB code: T00064
| RLCP classification | 3.900.45300.71 : Transfer | |
|---|---|---|
| CATH domain | 2.60.120.200 : Jelly Rolls | |
| 2.120.10.10 : Neuraminidase | Catalytic domain | |
| 2.40.220.10 : Intramolecular trans-sialidase; domain 3 | ||
| E.C. | 4.2.2.15 | |
| CSA | 1sll | |
| M-CSA | 1sll | |
| MACiE | ||
| CATH domain | Related DB codes (homologues) |
|---|---|
| 2.120.10.10 : Neuraminidase | D00173 M00310 T00065 T00208 |
| 2.60.120.200 : Jelly Rolls | S00148 D00535 D00666 M00185 S00511 T00208 |
| Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | CAZy | Pfam |
|---|---|---|---|---|
| Q27701 |
Anhydrosialidase
|
EC
4.2.2.15
Sialidase L Anhydroneuraminidase |
CBM40
(Carbohydrate-Binding Module Family 40)
GH33 (Glycoside Hydrolase Family 33) |
PF02973
(Sialidase)
[Graphical View] |
| KEGG enzyme name |
|---|
|
anhydrosialidase
anhydroneuraminidase sialglycoconjugate N-acylneuraminylhydrolase (2,7-cyclizing) sialidase L |
| UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
|---|---|---|---|---|---|
| Q27701 | NANL_MACDE | Elimination of alpha-sialyl groups in N- acetylneuraminic acid glycosides, releasing 2,7-anhydro-alpha-N- acetylneuraminate. | Secreted, extracellular space. |
| KEGG Pathways | Map code | Pathways | E.C. |
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| Compound table | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Substrates | Products | Intermediates | |||||||
| KEGG-id | C00270 | C04521 | C00001 | ||||||
| E.C. | |||||||||
| Compound | N-Acetylneuraminate | 2,7-Anhydro-alpha-N-acetylneuraminic acid | H2O | ||||||
| Type | amide group,carbohydrate,carboxyl group | amide group,carbohydrate,carboxyl group | H2O | ||||||
| ChEBI |
17012 17012 |
15377 15377 |
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| PubChem |
439197 439197 |
440369 440369 |
22247451 962 22247451 962 |
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| 1sliA01 |
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Unbound | Unbound | ||
| 1sllA01 |
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Unbound | Unbound | ||
| 2sliA01 |
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Unbound | Unbound | ||
| 3sliA01 |
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Unbound | Unbound | ||
| 4sliA01 |
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Unbound | Unbound | ||
| 1sliA02 |
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Unbound | Unbound | Intermediate-analogue:DAN | |
| 1sllA02 |
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Unbound | Unbound | ||
| 2sliA02 |
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Unbound | Bound:SKD | ||
| 3sliA02 |
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Unbound | Bound:SKD | ||
| 4sliA02 |
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Analogue:CNP | Unbound | ||
| 1sliA03 |
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Unbound | Unbound | ||
| 1sllA03 |
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Unbound | Unbound | ||
| 2sliA03 |
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Unbound | Unbound | ||
| 3sliA03 |
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Unbound | Unbound | ||
| 4sliA03 |
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Unbound | Unbound | ||
| Reference for Active-site residues | ||
|---|---|---|
| resource | references | E.C. |
| Swiss-prot;Q27701 & literature [4] | ||
| Active-site residues | ||||||||||
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| PDB | Catalytic residues | Cofactor-binding residues | Modified residues | Main-chain involved in catalysis | Comment | |||||
| 1sliA01 |
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| 1sllA01 |
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| 2sliA01 |
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| 3sliA01 |
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| 4sliA01 |
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| 1sliA02 |
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ASP 318;GLU 595;TYR 713 | ||||
| 1sllA02 |
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ASP 318;GLU 595;TYR 713 | ||||
| 2sliA02 |
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ASP 318;GLU 595;TYR 713 | ||||
| 3sliA02 |
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ASP 318;GLU 595;TYR 713 | ||||
| 4sliA02 |
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ASP 318;GLU 595;TYR 713 | ||||
| 1sliA03 |
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| 1sllA03 |
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| 2sliA03 |
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| 3sliA03 |
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| 4sliA03 |
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| References for Catalytic Mechanism | ||
|---|---|---|
| References | Sections | No. of steps in catalysis |
|
[2]
|
Fig.4, p.526-528 | |
|
[4]
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Fig.5, p.326-329 | |
| References | |
|---|---|
| [1] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2254319 |
| Journal | J Biol Chem |
| Year | 1990 |
| Volume | 265 |
| Pages | 21629-33 |
| Authors | Li YT, Nakagawa H, Ross SA, Hansson GC, Li SC |
| Title | A novel sialidase which releases 2,7-anhydro-alpha-N-acetylneuraminic acid from sialoglycoconjugates. |
| Related PDB | |
| Related UniProtKB | |
| [2] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 81-759. |
| Medline ID | |
| PubMed ID | 9562562 |
| Journal | Structure |
| Year | 1998 |
| Volume | 6 |
| Pages | 521-30 |
| Authors | Luo Y, Li SC, Chou MY, Li YT, Luo M |
| Title | The crystal structure of an intramolecular trans-sialidase with a NeuAc alpha2-->3Gal specificity. |
| Related PDB | 1sli 1sll |
| Related UniProtKB | Q27701 |
| [3] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10513893 |
| Journal | Biosci Rep |
| Year | 1999 |
| Volume | 19 |
| Pages | 163-8 |
| Authors | Sonnino S, Brocca P, Acquotti D, Bernardi A, Raimondi L, Kiso M, Ishida H, Li SC, Li YT |
| Title | The structural basis for the susceptibility of gangliosides to enzymatic degradation. |
| Related PDB | |
| Related UniProtKB | |
| [4] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 81-759. |
| Medline ID | |
| PubMed ID | 9878409 |
| Journal | J Mol Biol |
| Year | 1999 |
| Volume | 285 |
| Pages | 323-32 |
| Authors | Luo Y, Li SC, Li YT, Luo M |
| Title | The 1.8 A structures of leech intramolecular trans-sialidase complexes: evidence of its enzymatic mechanism. |
| Related PDB | 2sli 3sli 4sli |
| Related UniProtKB | Q27701 |
| Comments |
|---|
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According to the literature [2] & [4], (1) Tyr713 acts as a modulator, (2) Asp318 may act as a general acid to protonate the leaving O2 atom (probably through a water). (3) The departure of the leaving group leads to the oxocarbonium intermediate with a positive charge formed on C2 atom, (4) Asp318 acts as a general base, (5) The activated O7 atom makes a nucleophilic attack on the C2 atom to complete the reaction. |
| Created | Updated |
|---|---|
| 2005-04-18 | 2009-02-26 |