DB code: T00008
| CATH domain | 1.10.540.10 : Butyryl-Coa Dehydrogenase, subunit A; domain 1 | |
|---|---|---|
| 2.40.110.10 : Butyryl-CoA Dehydrogenase, subunit A; domain 2 | ||
| 1.20.140.10 : Butyryl-CoA Dehydrogenase, subunit A; domain 3 | Catalytic domain | |
| E.C. | 1.3.8.7 | |
| CSA | 3mdd | |
| M-CSA | 3mdd | |
| MACiE | ||
| CATH domain | Related DB codes (homologues) |
|---|---|
| 1.10.540.10 : Butyryl-Coa Dehydrogenase, subunit A; domain 1 | T00007 T00009 |
| 1.20.140.10 : Butyryl-CoA Dehydrogenase, subunit A; domain 3 | T00007 T00009 |
| 2.40.110.10 : Butyryl-CoA Dehydrogenase, subunit A; domain 2 | T00007 T00009 |
| Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | RefSeq | Pfam |
|---|---|---|---|---|
| Q72JJ3 |
|
Putative acyl-CoA dehydrogenase
EC 1.3.99.- |
YP_004752.1
(Protein)
NC_005835.1 (DNA/RNA sequence) |
PF00441
(Acyl-CoA_dh_1)
PF02770 (Acyl-CoA_dh_M) PF02771 (Acyl-CoA_dh_N) [Graphical View] |
| Q5SGZ2 |
|
Acyl-CoA dehydrogenase
|
YP_145204.1
(Protein)
NC_006461.1 (DNA/RNA sequence) |
PF00441
(Acyl-CoA_dh_1)
PF02770 (Acyl-CoA_dh_M) PF02771 (Acyl-CoA_dh_N) [Graphical View] |
| P41367 |
Medium-chain specific acyl-CoA dehydrogenase, mitochondrial
|
MCAD
EC 1.3.99.3 |
NP_999204.1
(Protein)
NM_214039.1 (DNA/RNA sequence) |
PF00441
(Acyl-CoA_dh_1)
PF02770 (Acyl-CoA_dh_M) PF02771 (Acyl-CoA_dh_N) [Graphical View] |
| P11310 |
Medium-chain specific acyl-CoA dehydrogenase, mitochondrial
|
MCAD
EC 1.3.99.3 |
NP_000007.1
(Protein)
NM_000016.4 (DNA/RNA sequence) NP_001120800.1 (Protein) NM_001127328.1 (DNA/RNA sequence) |
PF00441
(Acyl-CoA_dh_1)
PF02770 (Acyl-CoA_dh_M) PF02771 (Acyl-CoA_dh_N) [Graphical View] |
| KEGG enzyme name |
|---|
|
medium-chain acyl-CoA dehydrogenase
fatty acyl coenzyme A dehydrogenase (ambiguous) acyl coenzyme A dehydrogenase (ambiguous) acyl dehydrogenase (ambiguous) fatty-acyl-CoA dehydrogenase (ambiguous) acyl CoA dehydrogenase (ambiguous) general acyl CoA dehydrogenase (ambiguous) medium-chain acyl-coenzyme A dehydrogenase acyl-CoA:(acceptor) 2,3-oxidoreductase (ambiguous) ACADM (gene name) |
| UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
|---|---|---|---|---|---|
| Q72JJ3 | Q72JJ3_THET2 | ||||
| Q5SGZ2 | Q5SGZ2_THET8 | ||||
| P41367 | ACADM_PIG | A medium-chain acyl-CoA + electron-transfer flavoprotein = a medium-chain trans-2,3-dehydroacyl-CoA + reduced electron-transfer flavoprotein. | Homotetramer. | Mitochondrion matrix. | FAD. |
| P11310 | ACADM_HUMAN | A medium-chain acyl-CoA + electron-transfer flavoprotein = a medium-chain trans-2,3-dehydroacyl-CoA + reduced electron-transfer flavoprotein. | Homotetramer. Interacts with the heterodimeric electron transfer flavoprotein ETF. | Mitochondrion matrix. | FAD. |
| KEGG Pathways | Map code | Pathways | E.C. |
|---|---|---|
| MAP00071 | Fatty acid metabolism | |
| MAP00280 | Valine, leucine and isoleucine degradation | |
| MAP00410 | beta-Alanine metabolism | |
| MAP00640 | Propanoate metabolism |
| Compound table | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Cofactors | Substrates | Products | Intermediates | ||||||||
| KEGG-id | C00016 | C00040 | C04253 | C00605 | C04570 | ||||||
| E.C. | |||||||||||
| Compound | FAD | Acyl-CoA | Electron-transferring flavoprotein | 2,3-Dehydroacyl-CoA | Reduced electron-transferring flavoprotein | ||||||
| Type | amide group,amine group,aromatic ring (only carbon atom),aromatic ring (with nitrogen atoms),carbohydrate,nucleotide | amine group,carbohydrate,nucleotide ,peptide/protein,sulfide group | amide group,amine group,aromatic ring (only carbon atom),aromatic ring (with nitrogen atoms),carbohydrate,peptide/protein,phosphate group/phosphate ion | amine group,carbohydrate,nucleotide ,peptide/protein,sulfide group | amide group,amine group,aromatic ring (only carbon atom),aromatic ring (with nitrogen atoms),carbohydrate,peptide/protein,phosphate group/phosphate ion | ||||||
| ChEBI |
16238 16238 |
||||||||||
| PubChem |
643975 643975 |
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| 1ukwA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ukwB01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2cx9A01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2cx9B01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2cx9C01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2cx9D01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1udyA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1udyB01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1udyC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1udyD01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 3mddA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 3mddB01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 3mdeA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 3mdeB01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1egcA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1egcB01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1egcC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1egcD01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1egdA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1egdB01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1egdC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1egdD01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1egeA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1egeB01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1egeC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1egeD01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1t9gA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1t9gB01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1t9gC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1t9gD01 |
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Unbound | Unbound | Bound:TYR_192-ALA_193-THR_194-LEU_195 (chain S) | Unbound | Unbound | |
| 2a1tA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2a1tB01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2a1tC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2a1tD01 |
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Unbound | Unbound | Bound:TYR_192-ALA_193-THR_194-LEU_195 (chain S) | Unbound | Unbound | |
| 1ukwA02 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | |
| 1ukwB02 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | |
| 2cx9A02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2cx9B02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2cx9C02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2cx9D02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1udyA02 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | |
| 1udyB02 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | |
| 1udyC02 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | |
| 1udyD02 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | |
| 3mddA02 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | |
| 3mddB02 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | |
| 3mdeA02 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | |
| 3mdeB02 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | |
| 1egcA02 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | |
| 1egcB02 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | |
| 1egcC02 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | |
| 1egcD02 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | |
| 1egdA02 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | |
| 1egdB02 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | |
| 1egdC02 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | |
| 1egdD02 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | |
| 1egeA02 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | |
| 1egeB02 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | |
| 1egeC02 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | |
| 1egeD02 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | |
| 1t9gA02 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | |
| 1t9gB02 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | |
| 1t9gC02 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | |
| 1t9gD02 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | |
| 2a1tA02 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | |
| 2a1tB02 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | |
| 2a1tC02 |
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Bound:FAD | Unbound | Bound:FAD(chain R) | Unbound | Unbound | |
| 2a1tD02 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | |
| 1ukwA03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ukwB03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2cx9A03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2cx9B03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2cx9C03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2cx9D03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1udyA03 |
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Unbound | Analogue:CS8 | Unbound | Unbound | Unbound | |
| 1udyB03 |
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Unbound | Analogue:CS8 | Unbound | Unbound | Unbound | |
| 1udyC03 |
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Unbound | Analogue:CS8 | Unbound | Unbound | Unbound | |
| 1udyD03 |
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Unbound | Analogue:CS8 | Unbound | Unbound | Unbound | |
| 3mddA03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 3mddB03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 3mdeA03 |
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Unbound | Bound:CO8 | Unbound | Unbound | Unbound | |
| 3mdeB03 |
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Unbound | Bound:CO8 | Unbound | Unbound | Unbound | |
| 1egcA03 |
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Unbound | Bound:CO8 | Unbound | Unbound | Unbound | |
| 1egcB03 |
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Unbound | Bound:CO8 | Unbound | Unbound | Unbound | |
| 1egcC03 |
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Unbound | Bound:CO8 | Unbound | Unbound | Unbound | |
| 1egcD03 |
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Unbound | Bound:CO8 | Unbound | Unbound | Unbound | |
| 1egdA03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1egdB03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1egdC03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1egdD03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1egeA03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1egeB03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1egeC03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1egeD03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1t9gA03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1t9gB03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1t9gC03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1t9gD03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2a1tA03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2a1tB03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2a1tC03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2a1tD03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| Reference for Active-site residues | ||
|---|---|---|
| resource | references | E.C. |
| PDB;1egc, 1egd, 1ege, 3mde & Swiss-prot;P11310, P41367 & literature [6], [15], [21], [24], [32], [35], [39], [54] | ||
| References for Catalytic Mechanism | ||
|---|---|---|
| References | Sections | No. of steps in catalysis |
|
[1]
|
Scheme 2 | |
|
[4]
|
p.417 | |
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[15]
|
p.7525-7527 | |
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[19]
|
Scheme 1 | |
|
[21]
|
Scheme 3, p.719, p.722-723 | |
|
[22]
|
Fig.10, Fig.11, p.805-807 | |
|
[24]
|
p.12417-12420 | |
|
[30]
|
Scheme 1 | |
|
[31]
|
Fig.4, p.14609-14612 | |
|
[32]
|
p.1701-1704 | |
|
[35]
|
Scheme 1, Scheme 2, Scheme 5, p.8443-8444 | |
|
[36]
|
p.8454 | |
|
[37]
|
Scheme 1, p.1856-1860 | |
|
[38]
|
Fig.1, Fig.3 | |
|
[39]
|
Fig.1, p.226 | |
|
[40]
|
Scheme 4, p.264-266 | |
|
[41]
|
Scheme 1, Fig.7 | |
|
[42]
|
Scheme 2, p.13989-13991 | |
|
[45]
|
Scheme 1 | |
|
[50]
|
Scheme 1, p.4638 | |
|
[50]
|
Fig.8, p.4645-4647 | |
|
[53]
|
Scheme 1, Scheme 2, p.11852-11855 | |
|
[54]
|
p.301-303 | |
|
[55]
|
p.488-491 | |
|
[56]
|
||
|
[58]
|
Fig.5 | |
| References | |
|---|---|
| [1] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 3968064 |
| Journal | J Biol Chem |
| Year | 1985 |
| Volume | 260 |
| Pages | 1326-37 |
| Authors | Ikeda Y, Hine DG, Okamura-Ikeda K, Tanaka K |
| Title |
Mechanism of action of short-chain, |
| Related PDB | |
| Related UniProtKB | |
| [2] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 3769916 |
| Journal | Eur J Biochem |
| Year | 1986 |
| Volume | 160 |
| Pages | 109-15 |
| Authors | Pohl B, Raichle T, Ghisla S |
| Title |
Studies on the reaction mechanism of general acyl-CoA dehydrogenase. |
| Related PDB | |
| Related UniProtKB | |
| [3] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 3115254 |
| Journal | Biochem J |
| Year | 1987 |
| Volume | 243 |
| Pages | 519-24 |
| Authors | Steenkamp DJ |
| Title | Preferential cross-linking of the small subunit of the electron-transfer flavoprotein to general acyl-CoA dehydrogenase. |
| Related PDB | |
| Related UniProtKB | |
| [4] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 3053288 |
| Journal | Biochem Soc Trans |
| Year | 1988 |
| Volume | 16 |
| Pages | 416-8 |
| Authors | Frerman FE |
| Title |
Acyl-CoA dehydrogenases, |
| Related PDB | |
| Related UniProtKB | |
| [5] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 3413116 |
| Journal | Proc Natl Acad Sci U S A |
| Year | 1988 |
| Volume | 85 |
| Pages | 6677-81 |
| Authors | Kim JJ, Wu J |
| Title | Structure of the medium-chain acyl-CoA dehydrogenase from pig liver mitochondria at 3-A resolution. |
| Related PDB | |
| Related UniProtKB | |
| [6] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1970566 |
| Journal | J Biol Chem |
| Year | 1990 |
| Volume | 265 |
| Pages | 7116-9 |
| Authors | Bross P, Engst S, Strauss AW, Kelly DP, Rasched I, Ghisla S |
| Title | Characterization of wild-type and an active site mutant of human medium chain acyl-CoA dehydrogenase after expression in Escherichia coli. |
| Related PDB | |
| Related UniProtKB | |
| [7] | |
| Resource | |
| Comments | VARIANT MCAD GLU-329. |
| Medline ID | 91067682 |
| PubMed ID | 2251268 |
| Journal | Proc Natl Acad Sci U S A |
| Year | 1990 |
| Volume | 87 |
| Pages | 9236-40 |
| Authors | Kelly DP, Whelan AJ, Ogden ML, Alpers R, Zhang ZF, Bellus G, Gregersen N, Dorland L, Strauss AW |
| Title | Molecular characterization of inherited medium-chain acyl-CoA dehydrogenase deficiency. |
| Related PDB | |
| Related UniProtKB | P11310 |
| [8] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2326312 |
| Journal | Prog Clin Biol Res |
| Year | 1990 |
| Volume | 321 |
| Pages | 569-76 |
| Authors | Kim JJ, Wu J |
| Title | Structural studies of medium-chain acyl-CoA dehydrogenase from pig liver mitochondria. |
| Related PDB | |
| Related UniProtKB | |
| [9] | |
| Resource | |
| Comments | T00007-5 |
| Medline ID | |
| PubMed ID | 1931995 |
| Journal | Biochemistry |
| Year | 1991 |
| Volume | 30 |
| Pages | 10755-60 |
| Authors | Tserng KY, Jin SJ, Hoppel CL |
| Title | Spiropentaneacetic acid as a specific inhibitor of medium-chain acyl-CoA dehydrogenase. |
| Related PDB | |
| Related UniProtKB | |
| [10] | |
| Resource | |
| Comments | VARIANT MCAD GLU-329. |
| Medline ID | 91224627 |
| PubMed ID | 1902818 |
| Journal | Hum Genet |
| Year | 1991 |
| Volume | 86 |
| Pages | 545-51 |
| Authors | Gregersen N, Andresen BS, Bross P, Winter V, Rudiger N, Engst S, Christensen E, Kelly D, Strauss AW, Kolvraa S, et al |
| Title |
Molecular characterization of medium-chain acyl-CoA dehydrogenase (MCAD) deficiency: identification of a lys329 to glu mutation in the MCAD gene, |
| Related PDB | |
| Related UniProtKB | P11310 |
| [11] | |
| Resource | |
| Comments | T00007-6 |
| Medline ID | |
| PubMed ID | 8102510 |
| Journal | Am J Hum Genet |
| Year | 1993 |
| Volume | 53 |
| Pages | 730-9 |
| Authors | Andresen BS, Bross P, Jensen TG, Winter V, Knudsen I, Kolvraa S, Jensen UB, Bolund L, Duran M, Kim JJ, et al |
| Title |
A rare disease-associated mutation in the medium-chain acyl-CoA dehydrogenase (MCAD) gene changes a conserved arginine, |
| Related PDB | |
| Related UniProtKB | |
| [12] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8218225 |
| Journal | Biochemistry |
| Year | 1993 |
| Volume | 32 |
| Pages | 11575-85 |
| Authors | Johnson JK, Kumar NR, Srivastava DK |
| Title | Microscopic pathway for the medium-chain fatty acyl CoA dehydrogenase catalyzed oxidative half-reaction: changes in the electronic structures of flavin and CoA derivatives during catalysis. |
| Related PDB | |
| Related UniProtKB | |
| [13] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8267794 |
| Journal | Biochemistry |
| Year | 1993 |
| Volume | 32 |
| Pages | 8004-13 |
| Authors | Johnson JK, Srivastava DK |
| Title | Detection and identification of a chromophoric intermediate during the medium-chain fatty acyl-CoA dehydrogenase-catalyzed reaction via rapid-scanning UV/visible spectroscopy. |
| Related PDB | |
| Related UniProtKB | |
| [14] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8454567 |
| Journal | J Biochem (Tokyo) |
| Year | 1993 |
| Volume | 113 |
| Pages | 106-13 |
| Authors | Miura R, Nishina Y, Sato K, Fujii S, Kuroda K, Shiga K |
| Title | 13C- and 15N-NMR studies on medium-chain acyl-CoA dehydrogenase reconstituted with 13C- and 15N-enriched flavin adenine dinucleotide. |
| Related PDB | |
| Related UniProtKB | |
| [15] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS). |
| Medline ID | 93361479 |
| PubMed ID | 8356049 |
| Journal | Proc Natl Acad Sci U S A |
| Year | 1993 |
| Volume | 90 |
| Pages | 7523-7 |
| Authors | Kim JJ, Wang M, Paschke R |
| Title | Crystal structures of medium-chain acyl-CoA dehydrogenase from pig liver mitochondria with and without substrate. |
| Related PDB | 3mdd 3mde |
| Related UniProtKB | P41367 |
| [16] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8161532 |
| Journal | Biochemistry |
| Year | 1994 |
| Volume | 33 |
| Pages | 4738-44 |
| Authors | Johnson JK, Kumar NR, Srivastava DK |
| Title | Molecular basis of the medium-chain fatty acyl-CoA dehydrogenase-catalyzed "oxidase" reaction: pH-dependent distribution of intermediary enzyme species during catalysis. |
| Related PDB | |
| Related UniProtKB | |
| [17] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7905878 |
| Journal | J Biol Chem |
| Year | 1994 |
| Volume | 269 |
| Pages | 4401-8 |
| Authors | Saijo T, Welch WJ, Tanaka K |
| Title |
Intramitochondrial folding and assembly of medium-chain acyl-CoA dehydrogenase (MCAD). |
| Related PDB | |
| Related UniProtKB | |
| [18] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7703241 |
| Journal | Biochemistry |
| Year | 1995 |
| Volume | 34 |
| Pages | 4276-86 |
| Authors | Fitzsimmons ME, Thorpe C, Anders MW |
| Title | Medium-chain acyl-CoA dehydrogenase- and enoyl-CoA hydratase-dependent bioactivation of 5,6-dichloro-4-thia-5-hexenoyl-CoA. |
| Related PDB | |
| Related UniProtKB | |
| [19] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8845370 |
| Journal | Biochemistry |
| Year | 1995 |
| Volume | 34 |
| Pages | 16424-32 |
| Authors | Schaller RA, Thorpe C |
| Title | Oxidative inactivation of a charge transfer complex in the medium-chain acyl-CoA dehydrogenase. |
| Related PDB | |
| Related UniProtKB | |
| [20] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7718565 |
| Journal | Biochemistry |
| Year | 1995 |
| Volume | 34 |
| Pages | 4625-32 |
| Authors | Srivastava DK, Kumar NR, Peterson KL |
| Title | "Dehydrogenase" and "oxidase" reactions of medium-chain fatty acyl-CoA dehydrogenase utilizing chromogenic substrates: role of the 3',5'-adenosine diphosphate moiety of the coenzyme A thioester in catalysis. |
| Related PDB | |
| Related UniProtKB | |
| [21] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7601336 |
| Journal | FASEB J |
| Year | 1995 |
| Volume | 9 |
| Pages | 718-25 |
| Authors | Thorpe C, Kim JJ |
| Title | Structure and mechanism of action of the acyl-CoA dehydrogenases. |
| Related PDB | |
| Related UniProtKB | |
| [22] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7592542 |
| Journal | J Biochem (Tokyo) |
| Year | 1995 |
| Volume | 117 |
| Pages | 800-8 |
| Authors | Nishina Y, Sato K, Hazekawa I, Shiga K |
| Title | Structural modulation of 2-enoyl-CoA bound to reduced acyl-CoA dehydrogenases: a resonance Raman study of a catalytic intermediate. |
| Related PDB | |
| Related UniProtKB | |
| [23] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7730333 |
| Journal | J Biol Chem |
| Year | 1995 |
| Volume | 270 |
| Pages | 10284-90 |
| Authors | Bross P, Jespersen C, Jensen TG, Andresen BS, Kristensen MJ, Winter V, Nandy A, Krautle F, Ghisla S, Bolundi L, et al |
| Title |
Effects of two mutations detected in medium chain acyl-CoA dehydrogenase (MCAD)-deficient patients on folding, |
| Related PDB | |
| Related UniProtKB | |
| [24] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS). |
| Medline ID | 96420477 |
| PubMed ID | 8823176 |
| Journal | Biochemistry |
| Year | 1996 |
| Volume | 35 |
| Pages | 12412-20 |
| Authors | Lee HJ, Wang M, Paschke R, Nandy A, Ghisla S, Kim JJ |
| Title | Crystal structures of the wild type and the Glu376Gly/Thr255Glu mutant of human medium-chain acyl-CoA dehydrogenase: influence of the location of the catalytic base on substrate specificity. |
| Related PDB | 1egc 1egd 1ege |
| Related UniProtKB | P11310 |
| [25] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8823175 |
| Journal | Biochemistry |
| Year | 1996 |
| Volume | 35 |
| Pages | 12402-11 |
| Authors | Nandy A, Kieweg V, Krautle FG, Vock P, Kuchler B, Bross P, Kim JJ, Rasched I, Ghisla S |
| Title | Medium-long-chain chimeric human Acyl-CoA dehydrogenase: medium-chain enzyme with the active center base arrangement of long-chain Acyl-CoA dehydrogenase. |
| Related PDB | |
| Related UniProtKB | |
| [26] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9271097 |
| Journal | Biochem J |
| Year | 1997 |
| Volume | 325 |
| Pages | 751-60 |
| Authors | Peterson KL, Srivastava DK |
| Title | Functional role of a distal (3'-phosphate) group of CoA in the recombinant human liver medium-chain acyl-CoA dehydrogenase-catalysed reaction. |
| Related PDB | |
| Related UniProtKB | |
| [27] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9426198 |
| Journal | Biol Chem |
| Year | 1997 |
| Volume | 378 |
| Pages | 1381-5 |
| Authors | Macheroux P, Sanner C, Buttner H, Kieweg V, Ruterjans H, Ghisla S |
| Title | Medium-chain acyl CoA dehydrogenase: evidence for phosphorylation. |
| Related PDB | |
| Related UniProtKB | |
| [28] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9434899 |
| Journal | Curr Opin Struct Biol |
| Year | 1997 |
| Volume | 7 |
| Pages | 804-10 |
| Authors | Mattevi A, Vanoni MA, Curti B |
| Title | Structure of D-amino acid oxidase: new insights from an old enzyme. |
| Related PDB | |
| Related UniProtKB | |
| [29] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9208949 |
| Journal | Eur J Biochem |
| Year | 1997 |
| Volume | 246 |
| Pages | 548-56 |
| Authors | Kieweg V, Krautle FG, Nandy A, Engst S, Vock P, Abdel-Ghany AG, Bross P, Gregersen N, Rasched I, Strauss A, Ghisla S |
| Title |
Biochemical characterization of purified, |
| Related PDB | |
| Related UniProtKB | |
| [30] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9477967 |
| Journal | Biochemistry |
| Year | 1998 |
| Volume | 37 |
| Pages | 1383-93 |
| Authors | Baker-Malcolm JF, Haeffner-Gormley L, Wang L, Anders MW, Thorpe C |
| Title | Elimination reactions in the medium-chain acyl-CoA dehydrogenase: bioactivation of cytotoxic 4-thiaalkanoic acids. |
| Related PDB | |
| Related UniProtKB | |
| [31] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9772189 |
| Journal | Biochemistry |
| Year | 1998 |
| Volume | 37 |
| Pages | 14605-12 |
| Authors | Mancini-Samuelson GJ, Kieweg V, Sabaj KM, Ghisla S, Stankovich MT |
| Title |
Redox properties of human medium-chain acyl-CoA dehydrogenase, |
| Related PDB | |
| Related UniProtKB | |
| [32] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9484241 |
| Journal | Biochemistry |
| Year | 1998 |
| Volume | 37 |
| Pages | 1697-705 |
| Authors | Peterson KL, Galitz DS, Srivastava DK |
| Title | Influence of excision of a methylene group from Glu-376 (Glu376-->Asp mutation) in the medium chain acyl-CoA dehydrogenase-catalyzed reaction. |
| Related PDB | |
| Related UniProtKB | |
| [33] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9730839 |
| Journal | Biochemistry |
| Year | 1998 |
| Volume | 37 |
| Pages | 12659-71 |
| Authors | Peterson KL, Peterson KM, Srivastava DK |
| Title | Thermodynamics of ligand binding and catalysis in human liver medium-chain acyl-CoA dehydrogenase: comparative studies involving normal and 3'-dephosphorylated C8-CoAs and wild-type and Asn191 --> Ala (N191A) mutant enzymes. |
| Related PDB | |
| Related UniProtKB | |
| [34] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9521671 |
| Journal | Biochemistry |
| Year | 1998 |
| Volume | 37 |
| Pages | 3499-508 |
| Authors | Qin L, Srivastava DK |
| Title |
Energetics of two-step binding of a chromophoric reaction product, |
| Related PDB | |
| Related UniProtKB | |
| [35] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9622495 |
| Journal | Biochemistry |
| Year | 1998 |
| Volume | 37 |
| Pages | 8437-45 |
| Authors | Rudik I, Ghisla S, Thorpe C |
| Title | Protonic equilibria in the reductive half-reaction of the medium-chain acyl-CoA dehydrogenase. |
| Related PDB | |
| Related UniProtKB | |
| [36] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9622496 |
| Journal | Biochemistry |
| Year | 1998 |
| Volume | 37 |
| Pages | 8446-56 |
| Authors | Srivastava DK, Peterson KL |
| Title |
Discriminatory influence of Glu-376-->Asp mutation in medium-chain acyl-CoA dehydrogenase on the binding of selected CoA-ligands: spectroscopic, |
| Related PDB | |
| Related UniProtKB | |
| [37] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9485310 |
| Journal | Biochemistry |
| Year | 1998 |
| Volume | 37 |
| Pages | 1848-60 |
| Authors | Vock P, Engst S, Eder M, Ghisla S |
| Title | Substrate activation by acyl-CoA dehydrogenases: transition-state stabilization and pKs of involved functional groups. |
| Related PDB | |
| Related UniProtKB | |
| [38] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10496972 |
| Journal | Arch Biochem Biophys |
| Year | 1999 |
| Volume | 370 |
| Pages | 16-21 |
| Authors | Stankovich MT, Sabaj KM, Tonge PJ |
| Title | Structure/function of medium chain acyl-CoA dehydrogenase: the importance of substrate polarization. |
| Related PDB | |
| Related UniProtKB | |
| [39] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9882619 |
| Journal | Biochem J |
| Year | 1999 |
| Volume | 337 |
| Pages | 225-30 |
| Authors | Kuchler B, Abdel-Ghany AG, Bross P, Nandy A, Rasched I, Ghisla S |
| Title | Biochemical characterization of a variant human medium-chain acyl-CoA dehydrogenase with a disease-associated mutation localized in the active site. |
| Related PDB | |
| Related UniProtKB | |
| [40] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9890906 |
| Journal | Biochemistry |
| Year | 1999 |
| Volume | 38 |
| Pages | 257-67 |
| Authors | Engst S, Vock P, Wang M, Kim JJ, Ghisla S |
| Title | Mechanism of activation of acyl-CoA substrates by medium chain acyl-CoA dehydrogenase: interaction of the thioester carbonyl with the flavin adenine dinucleotide ribityl side chain. |
| Related PDB | |
| Related UniProtKB | |
| [41] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9990125 |
| Journal | J Biochem (Tokyo) |
| Year | 1999 |
| Volume | 125 |
| Pages | 285-96 |
| Authors | Tamaoki H, Nishina Y, Shiga K, Miura R |
| Title | Mechanism for the recognition and activation of substrate in medium-chain acyl-CoA dehydrogenase. |
| Related PDB | |
| Related UniProtKB | |
| [42] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11076541 |
| Journal | Biochemistry |
| Year | 2000 |
| Volume | 39 |
| Pages | 13982-92 |
| Authors | Pellett JD, Sabaj KM, Stephens AW, Bell AF, Wu J, Tonge PJ, Stankovich MT |
| Title |
Medium-chain acyl-coenzyme A dehydrogenase bound to a product analogue, |
| Related PDB | |
| Related UniProtKB | |
| [43] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11027146 |
| Journal | Biochemistry |
| Year | 2000 |
| Volume | 39 |
| Pages | 12659-70 |
| Authors | Peterson KM, Gopalan KV, Srivastava DK |
| Title | Influence of alpha-CH-->NH substitution in C8-CoA on the kinetics of association and dissociation of ligands with medium chain acyl-CoA dehydrogenase. |
| Related PDB | |
| Related UniProtKB | |
| [44] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11027148 |
| Journal | Biochemistry |
| Year | 2000 |
| Volume | 39 |
| Pages | 12678-87 |
| Authors | Peterson KM, Srivastava DK |
| Title | Energetic consequences of accommodating a bulkier ligand at the active site of medium chain acyl-CoA dehydrogenase by creating a complementary enzyme site cavity. |
| Related PDB | |
| Related UniProtKB | |
| [45] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10625483 |
| Journal | Biochemistry |
| Year | 2000 |
| Volume | 39 |
| Pages | 92-101 |
| Authors | Rudik I, Bell A, Tonge PJ, Thorpe C |
| Title | 4-Hydroxycinnamoyl-CoA: an ionizable probe of the active site of the medium chain acyl-CoA dehydrogenase. |
| Related PDB | |
| Related UniProtKB | |
| [46] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10694883 |
| Journal | Trends Biochem Sci |
| Year | 2000 |
| Volume | 25 |
| Pages | 126-32 |
| Authors | Fraaije MW, Mattevi A |
| Title | Flavoenzymes: diverse catalysts with recurrent features. |
| Related PDB | |
| Related UniProtKB | |
| [47] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11488611 |
| Journal | Arch Biochem Biophys |
| Year | 2001 |
| Volume | 392 |
| Pages | 341-8 |
| Authors | Rudik I, Thorpe C |
| Title | Thioester enolate stabilization in the acyl-CoA dehydrogenases: the effect of 5-deaza-flavin substitution. |
| Related PDB | |
| Related UniProtKB | |
| [48] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11591145 |
| Journal | Biochemistry |
| Year | 2001 |
| Volume | 40 |
| Pages | 12266-75 |
| Authors | Wang W, Fu Z, Zhou JZ, Kim JJ, Thorpe C |
| Title | Interaction of 3,4-dienoyl-CoA thioesters with medium chain acyl-CoA dehydrogenase: stereochemistry of inactivation of a flavoenzyme. |
| Related PDB | |
| Related UniProtKB | |
| [49] | |
| Resource | |
| Comments | VARIANT MCAD LEU-245. |
| Medline ID | 21302532 |
| PubMed ID | 11409868 |
| Journal | Hum Genet |
| Year | 2001 |
| Volume | 108 |
| Pages | 404-8 |
| Authors | Zschocke J, Schulze A, Lindner M, Fiesel S, Olgemoller K, Hoffmann GF, Penzien J, Ruiter JP, Wanders RJ, Mayatepek E |
| Title | Molecular and functional characterisation of mild MCAD deficiency. |
| Related PDB | |
| Related UniProtKB | P11310 |
| [50] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11926826 |
| Journal | Biochemistry |
| Year | 2002 |
| Volume | 41 |
| Pages | 4638-48 |
| Authors | Gopalan KV, Srivastava DK |
| Title | Beyond the proton abstracting role of Glu-376 in medium-chain acyl-CoA dehydrogenase: influence of Glu-376-->Gln substitution on ligand binding and catalysis. |
| Related PDB | |
| Related UniProtKB | |
| [51] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11872165 |
| Journal | J Biochem (Tokyo) |
| Year | 2002 |
| Volume | 131 |
| Pages | 365-74 |
| Authors | Nakajima Y, Miyahara I, Hirotsu K, Nishina Y, Shiga K, Setoyama C, Tamaoki H, Miura R |
| Title |
Three-dimensional structure of the flavoenzyme acyl-CoA oxidase-II from rat liver, |
| Related PDB | |
| Related UniProtKB | |
| [52] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12504892 |
| Journal | Arch Biochem Biophys |
| Year | 2003 |
| Volume | 409 |
| Pages | 251-61 |
| Authors | Lamm TR, Kohls TD, Saenger AK, Stankovich MT |
| Title | Comparison of ligand polarization and enzyme activation in medium- and short-chain acyl-coenzyme A dehydrogenase-novel analog complexes. |
| Related PDB | |
| Related UniProtKB | |
| [53] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 14529297 |
| Journal | Biochemistry |
| Year | 2003 |
| Volume | 42 |
| Pages | 11846-56 |
| Authors | Wu J, Bell AF, Luo L, Stephens AW, Stankovich MT, Tonge PJ |
| Title | Probing hydrogen-bonding interactions in the active site of medium-chain acyl-CoA dehydrogenase using Raman spectroscopy. |
| Related PDB | |
| Related UniProtKB | |
| [54] | |
| Resource | |
| Comments | X|ray crystallography |
| Medline ID | |
| PubMed ID | 12966080 |
| Journal | J Biochem (Tokyo) |
| Year | 2003 |
| Volume | 134 |
| Pages | 297-304 |
| Authors | Satoh A, Nakajima Y, Miyahara I, Hirotsu K, Tanaka T, Nishina Y, Shiga K, Tamaoki H, Setoyama C, Miura R |
| Title |
Structure of the transition state analog of medium-chain acyl-CoA dehydrogenase. |
| Related PDB | 1udy |
| Related UniProtKB | |
| [55] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 14728675 |
| Journal | Eur J Biochem |
| Year | 2004 |
| Volume | 271 |
| Pages | 483-93 |
| Authors | Kim JJ, Miura R |
| Title |
Acyl-CoA dehydrogenases and acyl-CoA oxidases. |
| Related PDB | |
| Related UniProtKB | |
| [56] | |
| Resource | |
| Comments | X|ray crystallography |
| Medline ID | |
| PubMed ID | 15159392 |
| Journal | J Biol Chem |
| Year | 2004 |
| Volume | 279 |
| Pages | 32904-12 |
| Authors | Toogood HS, van Thiel A, Basran J, Sutcliffe MJ, Scrutton NS, Leys D |
| Title | Extensive domain motion and electron transfer in the human electron transferring flavoprotein.medium chain Acyl-CoA dehydrogenase complex. |
| Related PDB | 1t9g |
| Related UniProtKB | |
| [57] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 15358373 |
| Journal | Protein Expr Purif |
| Year | 2004 |
| Volume | 37 |
| Pages | 472-8 |
| Authors | Zeng J, Li D |
| Title | Expression and purification of His-tagged rat mitochondrial medium-chain acyl-CoA dehydrogenase wild-type and Arg256 mutant proteins. |
| Related PDB | |
| Related UniProtKB | |
| [58] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 15975918 |
| Journal | J Biol Chem |
| Year | 2005 |
| Volume | 280 |
| Pages | 30361-6 |
| Authors | Toogood HS, van Thiel A, Scrutton NS, Leys D |
| Title | Stabilization of non-productive conformations underpins rapid electron transfer to electron-transferring flavoprotein. |
| Related PDB | 2a1t |
| Related UniProtKB | |
| Comments |
|---|
|
This enzyme is a member of medium-chain acyl-CoA dehydrogenases.
Accoriding to the literature, (A) Hydride transfer from acyl-CoA substrate to FAD, (B) Hydride transfer from FADH2 to FAD of an acceptor protein, The ETF protein is heterodimer (alpha/beta subunits) containing one FAD and one AMP per dimer (see [58], |
| Created | Updated |
|---|---|
| 2005-05-12 | 2012-10-02 |