DB code: M00004
| CATH domain | 3.30.43.10 : Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase; domain 2 | Catalytic domain |
|---|---|---|
| 3.30.465.20 : Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase; domain 3 | Catalytic domain | |
| 3.40.462.10 : Vanillyl-alcohol Oxidase; Chain A, domain 3 | Catalytic domain | |
| 1.10.45.10 : Vanillyl-alcohol Oxidase; Chain A, domain 4 | ||
| E.C. | 1.1.3.38 | |
| CSA | 1vao | |
| M-CSA | 1vao | |
| MACiE | M0103 | |
| CATH domain | Related DB codes (homologues) |
|---|---|
| 1.10.45.10 : Vanillyl-alcohol Oxidase; Chain A, domain 4 | M00179 |
| 3.30.43.10 : Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase; domain 2 | M00001 M00039 M00179 T00003 |
| 3.30.465.20 : Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase; domain 3 | M00001 M00179 |
| 3.40.462.10 : Vanillyl-alcohol Oxidase; Chain A, domain 3 | M00179 |
| Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | Pfam |
|---|---|---|---|
| P56216 |
Vanillyl-alcohol oxidase
|
EC
1.1.3.38
Aryl-alcohol oxidase 4-allylphenol oxidase |
PF02913
(FAD-oxidase_C)
PF01565 (FAD_binding_4) [Graphical View] |
| KEGG enzyme name |
|---|
|
vanillyl-alcohol oxidase
4-hydroxy-2-methoxybenzyl alcohol oxidase |
| UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
|---|---|---|---|---|---|
| P56216 | VAOX_PENSI | Vanillyl alcohol + O(2) = vanillin + H(2)O(2). | Homooctamer (tetramer of tightly interacting dimers). | Peroxisome. Cytoplasm. | Binds 1 FAD covalently per subunit. |
| KEGG Pathways | Map code | Pathways | E.C. |
|---|---|---|
| MAP00623 | 2,4-Dichlorobenzoate degradation |
| Compound table | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Cofactors | Substrates | Products | Intermediates | ||||||||
| KEGG-id | C00016 | C06317 | C00007 | C00755 | C00027 | ||||||
| E.C. | |||||||||||
| Compound | FAD | Vanillyl alcohol | O2 | Vanillin | H2O2 | p-quinone methide intermediate | |||||
| Type | amide group,amine group,aromatic ring (only carbon atom),aromatic ring (with nitrogen atoms),carbohydrate,nucleotide | aromatic ring (only carbon atom),carbohydrate | others | aromatic ring (only carbon atom),carbohydrate | others | ||||||
| ChEBI |
16238 16238 |
18353 18353 |
15379 26689 27140 15379 26689 27140 |
18346 18346 |
16240 16240 |
||||||
| PubChem |
643975 643975 |
62348 62348 |
977 977 |
1183 1183 |
22326046 784 22326046 784 |
||||||
| 1ahuA01 |
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Analogue:FAA | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1ahuB01 |
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Analogue:FAA | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1ahvA01 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1ahvB01 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1ahzA01 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1ahzB01 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1dznA01 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1dznB01 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1e0yA01 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1e0yB01 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1e8fA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1e8fB01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1e8gA01 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1e8gB01 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1e8hA01 |
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Analogue:ADP | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1e8hB01 |
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Analogue:ADP | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1qltA01 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1qltB01 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1qluA01 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1qluB01 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1vaoA01 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1vaoB01 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1w1jA01 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1w1jB01 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1w1kA01 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1w1kB01 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1w1lA01 |
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Analogue:EUG-FAD | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1w1lB01 |
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Analogue:EUG-FAD | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1w1mA01 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1w1mB01 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | Unbound |
| 2vaoA01 |
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | Unbound |
| 2vaoB01 |
|
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Bound:FAD | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1ahuA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1ahuB02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1ahvA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1ahvB02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1ahzA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1ahzB02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1dznA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1dznB02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1e0yA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1e0yB02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1e8fA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1e8fB02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1e8gA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1e8gB02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1e8hA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1e8hB02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1qltA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1qltB02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1qluA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1qluB02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1vaoA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1vaoB02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1w1jA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1w1jB02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1w1kA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1w1kB02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1w1lA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1w1lB02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1w1mA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1w1mB02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 2vaoA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 2vaoB02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1ahuA03 |
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Unbound | Analogue:FAA | Unbound | Unbound | Unbound | Unbound |
| 1ahuB03 |
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Unbound | Analogue:FAA | Unbound | Unbound | Unbound | Unbound |
| 1ahvA03 |
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Unbound | Analogue:NCR | Unbound | Unbound | Unbound | Unbound |
| 1ahvB03 |
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Unbound | Analogue:NCR | Unbound | Unbound | Unbound | Unbound |
| 1ahzA03 |
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Unbound | Analogue:EPT | Unbound | Unbound | Unbound | Unbound |
| 1ahzB03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1dznA03 |
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Unbound | Unbound | Unbound | Analogue:EUG | Unbound | Unbound |
| 1dznB03 |
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Unbound | Unbound | Unbound | Analogue:EUG | Unbound | Unbound |
| 1e0yA03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1e0yB03 |
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Unbound | Analogue:FCR | Unbound | Unbound | Unbound | Unbound |
| 1e8fA03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1e8fB03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1e8gA03 |
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Unbound | Analogue:FCR | Unbound | Unbound | Unbound | Unbound |
| 1e8gB03 |
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Unbound | Analogue:FCR | Unbound | Unbound | Unbound | Unbound |
| 1e8hA03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1e8hB03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1qltA03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1qltB03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1qluA03 |
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Unbound | Analogue:EUG | Unbound | Analogue:EUG | Unbound | Unbound |
| 1qluB03 |
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Unbound | Analogue:EUG | Unbound | Analogue:EUG | Unbound | Unbound |
| 1vaoA03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1vaoB03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1w1jA03 |
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Unbound | Unbound | Unbound | Analogue:EUG | Unbound | Unbound |
| 1w1jB03 |
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Unbound | Unbound | Unbound | Analogue:EUG | Unbound | Unbound |
| 1w1kA03 |
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Unbound | Unbound | Unbound | Analogue:EUG | Unbound | Unbound |
| 1w1kB03 |
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Unbound | Unbound | Unbound | Analogue:EUG | Unbound | Unbound |
| 1w1lA03 |
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Unbound | Unbound | Unbound | Analogue:EUG | Unbound | Unbound |
| 1w1lB03 |
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Unbound | Unbound | Unbound | Analogue:EUG | Unbound | Unbound |
| 1w1mA03 |
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Unbound | Unbound | Unbound | Analogue:EUG | Unbound | Unbound |
| 1w1mB03 |
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Unbound | Unbound | Unbound | Analogue:EUG | Unbound | Unbound |
| 2vaoA03 |
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Unbound | Unbound | Unbound | Analogue:EUG | Unbound | Unbound |
| 2vaoB03 |
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Unbound | Unbound | Unbound | Analogue:EUG | Unbound | Unbound |
| 1ahuA04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1ahuB04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1ahvA04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1ahvB04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1ahzA04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1ahzB04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1dznA04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1dznB04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1e0yA04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1e0yB04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1e8fA04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1e8fB04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1e8gA04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1e8gB04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1e8hA04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1e8hB04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1qltA04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1qltB04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1qluA04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1qluB04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1vaoA04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1vaoB04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1w1jA04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1w1jB04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1w1kA04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1w1kB04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1w1lA04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1w1lB04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1w1mA04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1w1mB04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 2vaoA04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 2vaoB04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| Reference for Active-site residues | ||
|---|---|---|
| resource | references | E.C. |
| Swiss-prot;P56216 & literature [10] | ||
| Active-site residues | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| PDB | Catalytic residues | Cofactor-binding residues | Modified residues | Main-chain involved in catalysis | Comment | |||||
| 1ahuA01 |
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TYR 108 | ||||
| 1ahuB01 |
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TYR 108 | ||||
| 1ahvA01 |
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TYR 108 | ||||
| 1ahvB01 |
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TYR 108 | ||||
| 1ahzA01 |
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TYR 108 | ||||
| 1ahzB01 |
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TYR 108 | ||||
| 1dznA01 |
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TYR 108 | ||||
| 1dznB01 |
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TYR 108 | ||||
| 1e0yA01 |
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TYR 108 | ||||
| 1e0yB01 |
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TYR 108 | ||||
| 1e8fA01 |
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TYR 108 | mutant H61T | |||
| 1e8fB01 |
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TYR 108 | mutant H61T | |||
| 1e8gA01 |
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TYR 108 | mutant H61T | |||
| 1e8gB01 |
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TYR 108 | mutant H61T | |||
| 1e8hA01 |
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TYR 108 | mutant H61T | |||
| 1e8hB01 |
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TYR 108 | mutant H61T | |||
| 1qltA01 |
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TYR 108 | ||||
| 1qltB01 |
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TYR 108 | ||||
| 1qluA01 |
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TYR 108 | ||||
| 1qluB01 |
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TYR 108 | ||||
| 1vaoA01 |
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TYR 108 | ||||
| 1vaoB01 |
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TYR 108 | ||||
| 1w1jA01 |
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TYR 108 | ||||
| 1w1jB01 |
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TYR 108 | ||||
| 1w1kA01 |
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TYR 108 | ||||
| 1w1kB01 |
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TYR 108 | ||||
| 1w1lA01 |
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TYR 108 | ||||
| 1w1lB01 |
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TYR 108 | ||||
| 1w1mA01 |
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TYR 108 | ||||
| 1w1mB01 |
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TYR 108 | ||||
| 2vaoA01 |
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TYR 108 | ||||
| 2vaoB01 |
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TYR 108 | ||||
| 1ahuA02 |
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ASP 170 | ||||
| 1ahuB02 |
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ASP 170 | ||||
| 1ahvA02 |
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ASP 170 | ||||
| 1ahvB02 |
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ASP 170 | ||||
| 1ahzA02 |
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ASP 170 | ||||
| 1ahzB02 |
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ASP 170 | ||||
| 1dznA02 |
|
|
|
|
|
mutant D170S | ||||
| 1dznB02 |
|
|
|
|
|
mutant D170S | ||||
| 1e0yA02 |
|
|
|
|
|
mutant D170S | ||||
| 1e0yB02 |
|
|
|
|
|
mutant D170S | ||||
| 1e8fA02 |
|
|
|
|
|
ASP 170 | ||||
| 1e8fB02 |
|
|
|
|
|
ASP 170 | ||||
| 1e8gA02 |
|
|
|
|
|
ASP 170 | ||||
| 1e8gB02 |
|
|
|
|
|
ASP 170 | ||||
| 1e8hA02 |
|
|
|
|
|
ASP 170 | ||||
| 1e8hB02 |
|
|
|
|
|
ASP 170 | ||||
| 1qltA02 |
|
|
|
|
|
ASP 170 | ||||
| 1qltB02 |
|
|
|
|
|
ASP 170 | ||||
| 1qluA02 |
|
|
|
|
|
ASP 170 | ||||
| 1qluB02 |
|
|
|
|
|
ASP 170 | ||||
| 1vaoA02 |
|
|
|
|
|
ASP 170 | ||||
| 1vaoB02 |
|
|
|
|
|
ASP 170 | ||||
| 1w1jA02 |
|
|
|
|
|
ASP 170 | ||||
| 1w1jB02 |
|
|
|
|
|
ASP 170 | ||||
| 1w1kA02 |
|
|
|
|
|
ASP 170 | mutant I238T | |||
| 1w1kB02 |
|
|
|
|
|
ASP 170 | mutant I238T | |||
| 1w1lA02 |
|
|
|
|
|
ASP 170 | ||||
| 1w1lB02 |
|
|
|
|
|
ASP 170 | ||||
| 1w1mA02 |
|
|
|
|
|
ASP 170 | ||||
| 1w1mB02 |
|
|
|
|
|
ASP 170 | ||||
| 2vaoA02 |
|
|
|
|
|
ASP 170 | ||||
| 2vaoB02 |
|
|
|
|
|
ASP 170 | ||||
| 1ahuA03 |
|
|
|
|
|
TYR 503;ARG 504 | HIS 422(FAD binding) | |||
| 1ahuB03 |
|
|
|
|
|
TYR 503;ARG 504 | HIS 422(FAD binding) | |||
| 1ahvA03 |
|
|
|
|
|
TYR 503;ARG 504 | HIS 422(FAD binding) | |||
| 1ahvB03 |
|
|
|
|
|
TYR 503;ARG 504 | HIS 422(FAD binding) | |||
| 1ahzA03 |
|
|
|
|
|
TYR 503;ARG 504 | HIS 422(FAD binding) | |||
| 1ahzB03 |
|
|
|
|
|
TYR 503;ARG 504 | HIS 422(FAD binding) | |||
| 1dznA03 |
|
|
|
|
|
TYR 503;ARG 504 | HIS 422(FAD binding) | |||
| 1dznB03 |
|
|
|
|
|
TYR 503;ARG 504 | HIS 422(FAD binding) | |||
| 1e0yA03 |
|
|
|
|
|
TYR 503;ARG 504 | HIS 422(FAD binding) | mutant T457E | ||
| 1e0yB03 |
|
|
|
|
|
TYR 503;ARG 504 | HIS 422(FAD binding) | mutant T457E | ||
| 1e8fA03 |
|
|
|
|
|
TYR 503;ARG 504 | HIS 422(FAD binding) | |||
| 1e8fB03 |
|
|
|
|
|
TYR 503;ARG 504 | HIS 422(FAD binding) | |||
| 1e8gA03 |
|
|
|
|
|
TYR 503;ARG 504 | HIS 422(FAD binding) | |||
| 1e8gB03 |
|
|
|
|
|
TYR 503;ARG 504 | HIS 422(FAD binding) | |||
| 1e8hA03 |
|
|
|
|
|
TYR 503;ARG 504 | HIS 422(FAD binding) | |||
| 1e8hB03 |
|
|
|
|
|
TYR 503;ARG 504 | HIS 422(FAD binding) | |||
| 1qltA03 |
|
|
|
|
|
TYR 503;ARG 504 | mutant H422A | |||
| 1qltB03 |
|
|
|
|
|
TYR 503;ARG 504 | mutant H422A | |||
| 1qluA03 |
|
|
|
|
|
TYR 503;ARG 504 | mutant H422A | |||
| 1qluB03 |
|
|
|
|
|
TYR 503;ARG 504 | mutant H422A | |||
| 1vaoA03 |
|
|
|
|
|
TYR 503;ARG 504 | HIS 422(FAD binding) | |||
| 1vaoB03 |
|
|
|
|
|
TYR 503;ARG 504 | HIS 422(FAD binding) | |||
| 1w1jA03 |
|
|
|
|
|
TYR 503;ARG 504 | HIS 422(FAD binding) | mutant T505S | ||
| 1w1jB03 |
|
|
|
|
|
TYR 503;ARG 504 | HIS 422(FAD binding) | mutant T505S | ||
| 1w1kA03 |
|
|
|
|
|
TYR 503;ARG 504 | HIS 422(FAD binding) | |||
| 1w1kB03 |
|
|
|
|
|
TYR 503;ARG 504 | HIS 422(FAD binding) | |||
| 1w1lA03 |
|
|
|
|
|
TYR 503;ARG 504 | HIS 422(FAD binding) | mutant F454Y | ||
| 1w1lB03 |
|
|
|
|
|
TYR 503;ARG 504 | HIS 422(FAD binding) | mutant F454Y | ||
| 1w1mA03 |
|
|
|
|
|
TYR 503;ARG 504 | HIS 422(FAD binding) | mutant E502G | ||
| 1w1mB03 |
|
|
|
|
|
TYR 503;ARG 504 | HIS 422(FAD binding) | mutant E502G | ||
| 2vaoA03 |
|
|
|
|
|
TYR 503;ARG 504 | HIS 422(FAD binding) | |||
| 2vaoB03 |
|
|
|
|
|
TYR 503;ARG 504 | HIS 422(FAD binding) | |||
| 1ahuA04 |
|
|
|
|
|
|||||
| 1ahuB04 |
|
|
|
|
|
|||||
| 1ahvA04 |
|
|
|
|
|
|||||
| 1ahvB04 |
|
|
|
|
|
|||||
| 1ahzA04 |
|
|
|
|
|
|||||
| 1ahzB04 |
|
|
|
|
|
|||||
| 1dznA04 |
|
|
|
|
|
|||||
| 1dznB04 |
|
|
|
|
|
|||||
| 1e0yA04 |
|
|
|
|
|
|||||
| 1e0yB04 |
|
|
|
|
|
|||||
| 1e8fA04 |
|
|
|
|
|
|||||
| 1e8fB04 |
|
|
|
|
|
|||||
| 1e8gA04 |
|
|
|
|
|
|||||
| 1e8gB04 |
|
|
|
|
|
|||||
| 1e8hA04 |
|
|
|
|
|
|||||
| 1e8hB04 |
|
|
|
|
|
|||||
| 1qltA04 |
|
|
|
|
|
|||||
| 1qltB04 |
|
|
|
|
|
|||||
| 1qluA04 |
|
|
|
|
|
|||||
| 1qluB04 |
|
|
|
|
|
|||||
| 1vaoA04 |
|
|
|
|
|
|||||
| 1vaoB04 |
|
|
|
|
|
|||||
| 1w1jA04 |
|
|
|
|
|
|||||
| 1w1jB04 |
|
|
|
|
|
|||||
| 1w1kA04 |
|
|
|
|
|
|||||
| 1w1kB04 |
|
|
|
|
|
|||||
| 1w1lA04 |
|
|
|
|
|
|||||
| 1w1lB04 |
|
|
|
|
|
|||||
| 1w1mA04 |
|
|
|
|
|
|||||
| 1w1mB04 |
|
|
|
|
|
|||||
| 2vaoA04 |
|
|
|
|
|
|||||
| 2vaoB04 |
|
|
|
|
|
|||||
| References for Catalytic Mechanism | ||
|---|---|---|
| References | Sections | No. of steps in catalysis |
|
[2]
|
p.276-277 | |
|
[4]
|
Equation 4, Scheme 1, p.18115-18116 | |
|
[6]
|
Fig.10, p.915-917 | |
|
[7]
|
Scheme 1, p.35518-35520 | |
|
[8]
|
Scheme 1, Fig.3, p.109 | |
|
[10]
|
Fig.1, Fig.8, p.14806-14807 | |
|
[11]
|
Scheme 1, Fig.5, p.9454-9456 | |
|
[13]
|
Fig.1 | |
|
[16]
|
Fig.2, p.182-186 | |
|
[17]
|
Fig.1, p.33498-33499 | |
| References | |
|---|---|
| [1] | |
| Resource | |
| Comments | CHARACTERIZATION |
| Medline ID | 93011092 |
| PubMed ID | 1396672 |
| Journal | Eur J Biochem |
| Year | 1992 |
| Volume | 208 |
| Pages | 651-7 |
| Authors | de Jong E, van Berkel WJ, van der Zwan RP, de Bont JA |
| Title |
Purification and characterization of vanillyl-alcohol oxidase from Penicillium simplicissimum. |
| Related PDB | |
| Related UniProtKB | P56216 |
| [2] | |
| Resource | |
| Comments | SUBSTRATE SPECIFICITY |
| Medline ID | 96096749 |
| PubMed ID | 8529652 |
| Journal | Eur J Biochem |
| Year | 1995 |
| Volume | 234 |
| Pages | 271-7 |
| Authors | Fraaije MW, Veeger C, van Berkel WJ |
| Title |
Substrate specificity of flavin-dependent vanillyl-alcohol oxidase from Penicillium simplicissimum. |
| Related PDB | |
| Related UniProtKB | P56216 |
| [3] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9013853 |
| Journal | FEBS Lett |
| Year | 1997 |
| Volume | 402 |
| Pages | 33-5 |
| Authors | Fraaije MW, Mattevi A, van Berkel WJ |
| Title | Mercuration of vanillyl-alcohol oxidase from Penicillium simplicissimum generates inactive dimers. |
| Related PDB | |
| Related UniProtKB | |
| [4] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9218444 |
| Journal | J Biol Chem |
| Year | 1997 |
| Volume | 272 |
| Pages | 18111-6 |
| Authors | Fraaije MW, van Berkel WJ |
| Title |
Catalytic mechanism of the oxidative demethylation of 4-(methoxymethyl)phenol by vanillyl-alcohol oxidase. |
| Related PDB | |
| Related UniProtKB | |
| [5] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.5 AND 3.2 ANGSTROMS) |
| Medline ID | 97285922 |
| PubMed ID | 9141139 |
| Journal | Proteins |
| Year | 1997 |
| Volume | 27 |
| Pages | 601-3 |
| Authors | Mattevi A, Fraaije MW, Coda A, van Berkel WJ |
| Title | Crystallization and preliminary X-ray analysis of the flavoenzyme vanillyl-alcohol oxidase from Penicillium simplicissimum. |
| Related PDB | 1ahu 1ahv 1ahz 1vao 2vao |
| Related UniProtKB | P56216 |
| [6] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9261083 |
| Journal | Structure |
| Year | 1997 |
| Volume | 5 |
| Pages | 907-20 |
| Authors | Mattevi A, Fraaije MW, Mozzarelli A, Olivi L, Coda A, van Berkel WJ |
| Title | Crystal structures and inhibitor binding in the octameric flavoenzyme vanillyl-alcohol oxidase: the shape of the active-site cavity controls substrate specificity. |
| Related PDB | |
| Related UniProtKB | |
| [7] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY OF MUTANTS (2.2 ANGSTROMS) |
| Medline ID | 20054426 |
| PubMed ID | 10585424 |
| Journal | J Biol Chem |
| Year | 1999 |
| Volume | 274 |
| Pages | 35514-20 |
| Authors | Fraaije MW, van den Heuvel RH, van Berkel WJ, Mattevi A |
| Title | Covalent flavinylation is essential for efficient redox catalysis in vanillyl-alcohol oxidase. |
| Related PDB | 1qlt 1qlu |
| Related UniProtKB | P56216 |
| [8] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10996306 |
| Journal | FEBS Lett |
| Year | 2000 |
| Volume | 481 |
| Pages | 109-12 |
| Authors | van den Heuvel RH, Fraaije MW, van Berkel WJ |
| Title | Direction of the reactivity of vanillyl-alcohol oxidase with 4-alkylphenols. |
| Related PDB | |
| Related UniProtKB | |
| [9] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY OF MUTANTS (2.1 ANGSTROMS) |
| Medline ID | 20556311 |
| PubMed ID | 10984479 |
| Journal | J Biol Chem |
| Year | 2000 |
| Volume | 275 |
| Pages | 38654-8 |
| Authors | Fraaije MW, van Den Heuvel RH, van Berkel WJ, Mattevi A |
| Title | Structural analysis of flavinylation in vanillyl-alcohol oxidase. |
| Related PDB | 1e8f 1e8g 1e8h |
| Related UniProtKB | P56216 |
| [10] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY OF D170S MUTANT (2.8 ANGSTROMS) |
| Medline ID | 20270176 |
| PubMed ID | 10809721 |
| Journal | J Biol Chem |
| Year | 2000 |
| Volume | 275 |
| Pages | 14799-808 |
| Authors | van den Heuvel RH, Fraaije MW, Mattevi A, van Berkel WJ |
| Title | Asp-170 is crucial for the redox properties of vanillyl-alcohol oxidase. |
| Related PDB | 1dzn |
| Related UniProtKB | P56216 |
| [11] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY OF D170S/T457E MUTANT (2.75 ANGSTROMS) |
| Medline ID | 20402555 |
| PubMed ID | 10920192 |
| Journal | Proc Natl Acad Sci U S A |
| Year | 2000 |
| Volume | 97 |
| Pages | 9455-60 |
| Authors | van Den Heuvel RH, Fraaije MW, Ferrer M, Mattevi A, van Berkel WJ |
| Title | Inversion of stereospecificity of vanillyl-alcohol oxidase. |
| Related PDB | 1e0y |
| Related UniProtKB | P56216 |
| [12] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10752605 |
| Journal | Protein Sci |
| Year | 2000 |
| Volume | 9 |
| Pages | 435-9 |
| Authors | van Berkel WJ, van den Heuvel RH, Versluis C, Heck AJ |
| Title | Detection of intact megaDalton protein assemblies of vanillyl-alcohol oxidase by mass spectrometry. |
| Related PDB | |
| Related UniProtKB | |
| [13] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10694883 |
| Journal | Trends Biochem Sci |
| Year | 2000 |
| Volume | 25 |
| Pages | 126-32 |
| Authors | Fraaije MW, Mattevi A |
| Title | Flavoenzymes: diverse catalysts with recurrent features. |
| Related PDB | |
| Related UniProtKB | |
| [14] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11513884 |
| Journal | FEBS Lett |
| Year | 2001 |
| Volume | 503 |
| Pages | 213-6 |
| Authors | van den Heuvel RH, Partridge J, Laane C, Halling PJ, van Berkel WJ |
| Title | Tuning of the product spectrum of vanillyl-alcohol oxidase by medium engineering. |
| Related PDB | |
| Related UniProtKB | |
| [15] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12107187 |
| Journal | J Biol Chem |
| Year | 2002 |
| Volume | 277 |
| Pages | 36425-32 |
| Authors | Tahallah N, Van Den Heuvel RH, Van Den Berg WA, Maier CS, Van Berkel WJ, Heck AJ |
| Title | Cofactor-dependent assembly of the flavoenzyme vanillyl-alcohol oxidase. |
| Related PDB | |
| Related UniProtKB | |
| [16] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12078493 |
| Journal | Methods Enzymol |
| Year | 2002 |
| Volume | 353 |
| Pages | 177-86 |
| Authors | van den Heuvel RH, Fraaije MW, van Berkel WJ |
| Title | Redox properties of vanillyl-alcohol oxidase. |
| Related PDB | |
| Related UniProtKB | |
| [17] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 15169773 |
| Journal | J Biol Chem |
| Year | 2004 |
| Volume | 279 |
| Pages | 33492-500 |
| Authors | van den Heuvel RH, van den Berg WA, Rovida S, van Berkel WJ |
| Title | Laboratory-evolved vanillyl-alcohol oxidase produces natural vanillin. |
| Related PDB | 1w1j 1w1k 1w1l 1w1m |
| Related UniProtKB | |
| Comments |
|---|
|
Although vanillyl alcohol (C06317) is annotated as a substrate, According to the literature [10], (A) Hydride transfer from methyl group of substrate to FAD, (B) Hydride transfer from FADH2 to O2 (Re-oxidation of FAD by O2, (C) Additive double-bond deformation; Addition of H2O to p-quinone methide intermediate (Hydroxylation): (D) Eliminative double-bond formation; Elimination of H2O (or Elimination of methanol in the case of 4-(methoxymethyl)phenol as a substrate): |
| Created | Updated |
|---|---|
| 2005-06-01 | 2009-02-26 |