DB code: D00235
| RLCP classification | 1.13.10900.463 : Hydrolysis | |
|---|---|---|
| CATH domain | 3.10.170.10 : Elastase; domain 1 | Catalytic domain |
| 1.10.390.10 : Neutral Protease; domain 2 | Catalytic domain | |
| E.C. | 3.4.24.28 | |
| CSA | ||
| M-CSA | ||
| MACiE | ||
| CATH domain | Related DB codes (homologues) |
|---|---|
| 1.10.390.10 : Neutral Protease; domain 2 | D00233 D00234 D00480 |
| 3.10.170.10 : Elastase; domain 1 | D00233 D00234 D00480 |
| Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | MEROPS | Pfam |
|---|---|---|---|---|
| P05806 |
Bacillolysin
|
EC
3.4.24.28
Neutral protease |
M04.001
(Metallo)
|
PF07504
(FTP)
PF03413 (PepSY) PF01447 (Peptidase_M4) PF02868 (Peptidase_M4_C) [Graphical View] |
| KEGG enzyme name |
|---|
|
bacillolysin
Bacillus metalloendopeptidase Bacillus subtilis neutral proteinase anilozyme P 10 Bacillus metalloproteinase Bacillus neutral proteinase megateriopeptidase |
| UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
|---|---|---|---|---|---|
| P05806 | NPRE_BACCE | Similar, but not identical, to that of thermolysin. | Secreted. | Binds 4 calcium ions per subunit. Binds 1 zinc ion per subunit. |
| KEGG Pathways | Map code | Pathways | E.C. |
|---|
| Compound table | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cofactors | Substrates | Products | Intermediates | |||||||||
| KEGG-id | C00038 | C00017 | C00012 | C00001 | C00017 | C00012 | ||||||
| E.C. | ||||||||||||
| Compound | Zinc | Protein | Peptide | H2O | Protein | Peptide | ||||||
| Type | heavy metal | peptide/protein | peptide/protein | H2O | peptide/protein | peptide/protein | ||||||
| ChEBI |
29105 29105 |
15377 15377 |
||||||||||
| PubChem |
32051 32051 |
22247451 962 22247451 962 |
||||||||||
| 1espA01 |
|
|
|
|
|
Bound:_ZN | Unbound | Unbound | Unbound | Unbound | ||
| 1npcA01 |
|
|
|
|
|
Bound:_ZN | Unbound | Unbound | Unbound | Unbound | ||
| 1espA02 |
|
|
|
|
|
Bound:_ZN | Unbound | Unbound | Unbound | Unbound | ||
| 1npcA02 |
|
|
|
|
|
Bound:_ZN | Unbound | Unbound | Unbound | Unbound | ||
| Reference for Active-site residues | ||
|---|---|---|
| resource | references | E.C. |
| PDB;1esp, 1npc & Swiss-prot;P05806 | ||
| Active-site residues | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| PDB | Catalytic residues | Cofactor-binding residues | Modified residues | Main-chain involved in catalysis | Comment | |||||
| 1espA01 |
|
|
|
|
|
ASN 113; | HIS 143;HIS 147(Zinc binding) | ALA 114 | mutant E144S | |
| 1npcA01 |
|
|
|
|
|
ASN 113;GLU 144 | HIS 143;HIS 147(Zinc binding) | ALA 114 | ||
| 1espA02 |
|
|
|
|
|
TYR 158;HIS 232 | GLU 167(Zinc binding) | |||
| 1npcA02 |
|
|
|
|
|
TYR 158;HIS 232 | GLU 167(Zinc binding) | |||
| References for Catalytic Mechanism | ||
|---|---|---|
| References | Sections | No. of steps in catalysis |
| References | |
|---|---|
| [1] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS). |
| Medline ID | 88172498 |
| PubMed ID | 3127592 |
| Journal | J Mol Biol |
| Year | 1988 |
| Volume | 199 |
| Pages | 525-37 |
| Authors | Pauptit RA, Karlsson R, Picot D, Jenkins JA, Niklaus-Reimer AS, Jansonius JN |
| Title | Crystal structure of neutral protease from Bacillus cereus refined at 3.0 A resolution and comparison with the homologous but more thermostable enzyme thermolysin. |
| Related PDB | |
| Related UniProtKB | P05806 |
| [2] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2124807 |
| Journal | Biochem J |
| Year | 1990 |
| Volume | 272 |
| Pages | 93-7 |
| Authors | van den Burg B, Eijsink VG, Stulp BK, Venema G |
| Title | Identification of autodigestion target sites in Bacillus subtilis neutral proteinase. |
| Related PDB | |
| Related UniProtKB | |
| [3] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1899021 |
| Journal | Biochemistry |
| Year | 1991 |
| Volume | 30 |
| Pages | 97-106 |
| Authors | Toma S, Campagnoli S, Margarit I, Gianna R, Grandi G, Bolognesi M, De Filippis V, Fontana A |
| Title | Grafting of a calcium-binding loop of thermolysin to Bacillus subtilis neutral protease. |
| Related PDB | |
| Related UniProtKB | |
| [4] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1817257 |
| Journal | Protein Eng |
| Year | 1991 |
| Volume | 4 |
| Pages | 941-5 |
| Authors | Vriend G, Berendsen HJ, van der Zee JR, van den Burg B, Venema G, Eijsink VG |
| Title | Stabilization of the neutral protease of Bacillus stearothermophilus by removal of a buried water molecule. |
| Related PDB | |
| Related UniProtKB | |
| [5] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS). |
| Medline ID | 92339470 |
| PubMed ID | 1633827 |
| Journal | Eur J Biochem |
| Year | 1992 |
| Volume | 207 |
| Pages | 781-91 |
| Authors | Stark W, Pauptit RA, Wilson KS, Jansonius JN |
| Title | The structure of neutral protease from Bacillus cereus at 0.2-nm resolution. |
| Related PDB | 1npc |
| Related UniProtKB | P05806 |
| [6] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1518790 |
| Journal | Protein Eng |
| Year | 1992 |
| Volume | 5 |
| Pages | 421-6 |
| Authors | Eijsink VG, Dijkstra BW, Vriend G, van der Zee JR, Veltman OR, van der Vinne B, van den Burg B, Kempe S, Venema G |
| Title | The effect of cavity-filling mutations on the thermostability of Bacillus stearothermophilus neutral protease. |
| Related PDB | |
| Related UniProtKB | |
| [7] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1594570 |
| Journal | Protein Eng |
| Year | 1992 |
| Volume | 5 |
| Pages | 157-63 |
| Authors | Eijsink VG, Vriend G, van den Burg B, van der Zee JR, Veltman OR, Stulp BK, Venema G |
| Title | Introduction of a stabilizing 10 residue beta-hairpin in Bacillus subtilis neutral protease. |
| Related PDB | |
| Related UniProtKB | |
| [8] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1594571 |
| Journal | Protein Eng |
| Year | 1992 |
| Volume | 5 |
| Pages | 165-70 |
| Authors | Eijsink VG, Vriend G, van den Burg B, van der Zee JR, Venema G |
| Title | Increasing the thermostability of a neutral protease by replacing positively charged amino acids in the N-terminal turn of alpha-helices. |
| Related PDB | |
| Related UniProtKB | |
| [9] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8415578 |
| Journal | Protein Eng |
| Year | 1993 |
| Volume | 6 |
| Pages | 521-7 |
| Authors | van den Burg B, Dijkstra BW, van der Vinne B, Stulp BK, Eijsink VG, Venema G |
| Title | Introduction of disulfide bonds into Bacillus subtilis neutral protease. |
| Related PDB | |
| Related UniProtKB | |
| [10] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8143751 |
| Journal | Eur J Biochem |
| Year | 1994 |
| Volume | 220 |
| Pages | 981-5 |
| Authors | Van den Burg B, Dijkstra BW, Vriend G, Van der Vinne B, Venema G, Eijsink VG |
| Title | Protein stabilization by hydrophobic interactions at the surface. |
| Related PDB | |
| Related UniProtKB | |
| [11] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8177891 |
| Journal | Protein Eng |
| Year | 1994 |
| Volume | 7 |
| Pages | 425-30 |
| Authors | Hardy F, Vriend G, van der Vinne B, Frigerio F, Grandi G, Venema G, Eijsink VG |
| Title | The effect of engineering surface loops on the thermal stability of Bacillus subtilis neutral protease. |
| Related PDB | |
| Related UniProtKB | |
| [12] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF MUTANT SER-393. |
| Medline ID | |
| PubMed ID | |
| Journal | Acta Crystallogr D Biol Crystallogr |
| Year | 1996 |
| Volume | 52 |
| Pages | 543-50 |
| Authors | Lister SA, Wetmore DR, Roche RS, Codding PW |
| Title | E144S active-site mutant of the Bacillus cereus thermolysin-like neutral protease at 2.8-A resolution. |
| Related PDB | 1esp |
| Related UniProtKB | P05806 |
| [13] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9761816 |
| Journal | Acta Crystallogr D Biol Crystallogr |
| Year | 1998 |
| Volume | 54 |
| Pages | 45-57 |
| Authors | Carfi A, Duee E, Paul-Soto R, Galleni M, Frere JM, Dideberg O |
| Title | X-ray structure of the ZnII beta-lactamase from Bacteroides fragilis in an orthorhombic crystal form. |
| Related PDB | |
| Related UniProtKB | |
| [14] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9569607 |
| Journal | Biotechnol Appl Biochem |
| Year | 1998 |
| Volume | 27 |
| Pages | 125-32 |
| Authors | Van den Burg B, Eijsink VG, Vriend G, Veltman OR, Venema G |
| Title | Rendering one autolysis site in Bacillus subtilis neutral protease resistant to cleavage reveals a new fission. |
| Related PDB | |
| Related UniProtKB | |
| [15] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10611287 |
| Journal | Proc Natl Acad Sci U S A |
| Year | 1999 |
| Volume | 96 |
| Pages | 14765-70 |
| Authors | Rudner DZ, Fawcett P, Losick R |
| Title | A family of membrane-embedded metalloproteases involved in regulated proteolysis of membrane-associated transcription factors. |
| Related PDB | |
| Related UniProtKB | |
| Comments |
|---|
|
This enzyme belongs to peptidase family-M4 (Thermolysin family).
Although this enzyme binds four calcium ions, As the active site is the same as the thermolysin (D00234 in EzCatDB), |
| Created | Updated |
|---|---|
| 2005-04-13 | 2009-02-26 |