DB code: D00234
RLCP classification | 1.13.10900.463 : Hydrolysis | |
---|---|---|
CATH domain | 3.10.170.10 : Elastase; domain 1 | Catalytic domain |
1.10.390.10 : Neutral Protease; domain 2 | Catalytic domain | |
E.C. | 3.4.24.27 | |
CSA | 1tlp 8tln | |
M-CSA | 1tlp 8tln | |
MACiE | M0176 |
CATH domain | Related DB codes (homologues) |
---|---|
1.10.390.10 : Neutral Protease; domain 2 | D00233 D00235 D00480 |
3.10.170.10 : Elastase; domain 1 | D00233 D00235 D00480 |
Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | MEROPS | Pfam |
---|---|---|---|---|
P00800 |
Thermolysin
|
EC
3.4.24.27
Thermostable neutral proteinase |
M04.001
(Metallo)
|
PF07504
(FTP)
PF03413 (PepSY) PF01447 (Peptidase_M4) PF02868 (Peptidase_M4_C) [Graphical View] |
KEGG enzyme name |
---|
thermolysin
Bacillus thermoproteolyticus neutral proteinase thermoase thermoase Y10 TLN |
UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
---|---|---|---|---|---|
P00800 | THER_BACTH | Preferential cleavage: Xaa-|-Leu > Xaa-|-Phe. | Secreted. | Binds 4 calcium ions per subunit. Binds 1 zinc ion per subunit. |
KEGG Pathways | Map code | Pathways | E.C. |
---|
Compound table | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Cofactors | Substrates | Products | Intermediates | |||||||||
KEGG-id | C00038 | C00017 | C00012 | C00001 | C00017 | C00012 | ||||||
E.C. | ||||||||||||
Compound | Zinc | Protein | Peptide | H2O | Protein | Peptide(leaving group) | ||||||
Type | heavy metal | peptide/protein | peptide/protein | H2O | peptide/protein | peptide/protein | ||||||
ChEBI |
29105 29105 |
15377 15377 |
||||||||||
PubChem |
32051 32051 |
22247451 962 22247451 962 |
||||||||||
1fj3A01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Unbound | |
1fjoA01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Unbound | |
1fjqA01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Unbound | |
1fjtA01 |
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Bound:_ZN | Unbound | Unbound | Bound:HOH_202 | Unbound | Bound:VAL-LYS | Unbound |
1fjuA01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Unbound | |
1fjvA01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Unbound | |
1fjwA01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Unbound | |
1gxwA01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Bound:VAL-LYS | Unbound | |
1hytA01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:BZS | |
1keiA01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Bound:VAL-LYS | Unbound | |
1kjoA01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:PHQ-THR | |
1kjpA01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:PHQ-GLU | |
1kkkA01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:PHQ-ASP | |
1kl6A01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:PHQ-ALA | |
1kr6A01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:PHQ-DGL | |
1kroA01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:PHQ-DTH | |
1ks7A01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:PHQ-DAS | |
1ktoA01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Transition-state-Analogue:PHQ-DAL | |
1l3fE01 |
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Bound:_ZN_321 | Unbound | Unbound | Unbound | Unbound | Unbound | |
1lnaE01 |
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Analogue:_CO_900 | Unbound | Unbound | Unbound | Bound:VAL-LYS | Unbound | |
1lnbE01 |
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Analogue:_FE | Unbound | Unbound | Unbound | Bound:VAL-LYS | Unbound | |
1lncE01 |
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Analogue:_MN_900 | Unbound | Unbound | Unbound | Bound:VAL-LYS | Unbound | |
1lndE01 |
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Bound:_ZN_800 | Unbound | Unbound | Unbound | Bound:VAL-LYS | Unbound | |
1lneE01 |
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Analogue:_CD_900 | Unbound | Unbound | Unbound | Bound:VAL-LYS | Unbound | |
1lnfE01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Bound:VAL-LYS | Unbound | |
1os0A01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:PPH-DPH-PHE | |
1qf0A01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:TI2 | |
1qf1A01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:TI1 | |
1qf2A01 |
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Bound:_ZN | Unbound | Analogue:TI3 | Unbound | Unbound | Unbound | |
1thlA01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:CLT-CCM-TRP | |
1tliA01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Unbound | |
1tlpE01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:RHA-LEU-TRP | |
1tlxA01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Bound:VAL-LYS | Unbound | |
1tmnE01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:CLT-LEU-TRP | |
2tliA01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Unbound | |
2tlxA01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Bound:VAL-LYS | Unbound | |
2tmnE01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:PO3-LEU-NH2 | |
3tliA01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Unbound | |
3tlnA01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Unbound | |
3tmnE01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Bound:VAL-TRP | Unbound | |
4tliA01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Unbound | |
4tlnA01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:LNO | |
4tmnE01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:PHQ-PPH-LEU-ALA | |
5tliA01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Unbound | |
5tlnA01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:BAN | |
5tmnE01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:PHQ-PGL-LEU-LEU | |
6tliA01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Unbound | |
6tmnE01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Intermediate-analogue:PHQ-PGL-1LU-LEU | |
7tliA01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Unbound | |
7tlnA01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Intermediate-analogue:INC | |
8tliA01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Unbound | |
8tlnE01 |
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Bound:_ZN | Unbound | Unbound | Unbound | Bound:VAL-LYS | Unbound | |
1fj3A02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
1fjoA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
1fjqA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
1fjtA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
1fjuA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
1fjvA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
1fjwA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
1gxwA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
1hytA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
1keiA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
1kjoA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
1kjpA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
1kkkA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
1kl6A02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
1kr6A02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
1kroA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
1ks7A02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
1ktoA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
1l3fE02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
1lnaE02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
1lnbE02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
1lncE02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
1lndE02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
1lneE02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
1lnfE02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
1os0A02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
1qf0A02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
1qf1A02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
1qf2A02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
1thlA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
1tliA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
1tlpE02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
1tlxA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
1tmnE02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
1trlA |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
1trlB |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
2tliA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
2tlxA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
2tmnE02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
3tliA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
3tlnA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
3tmnE02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
4tliA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
4tlnA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
4tmnE02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
5tliA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
5tlnA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
5tmnE02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
6tliA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
6tmnE02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
7tliA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
7tlnA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
8tliA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
8tlnE02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
Reference for Active-site residues | ||
---|---|---|
resource | references | E.C. |
Swiss-prot;P00800 |
Active-site residues | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
PDB | Catalytic residues | Cofactor-binding residues | Modified residues | Main-chain involved in catalysis | Comment | |||||
1fj3A01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
1fjoA01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
1fjqA01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
1fjtA01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
1fjuA01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
1fjvA01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
1fjwA01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
1gxwA01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
1hytA01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
1keiA01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
1kjoA01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
1kjpA01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
1kkkA01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
1kl6A01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
1kr6A01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
1kroA01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
1ks7A01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
1ktoA01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
1l3fE01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
1lnaE01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
1lnbE01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
1lncE01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
1lndE01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
1lneE01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
1lnfE01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
1os0A01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
1qf0A01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
1qf1A01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
1qf2A01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
1thlA01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
1tliA01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
1tlpE01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
1tlxA01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
1tmnE01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
2tliA01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
2tlxA01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
2tmnE01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
3tliA01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
3tlnA01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
3tmnE01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
4tliA01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
4tlnA01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
4tmnE01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
5tliA01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
5tlnA01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
5tmnE01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
6tliA01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
6tmnE01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
7tliA01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
7tlnA01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
8tliA01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
8tlnE01 |
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ASN 112;GLU 143 | HIS 142;HIS 146(Zinc binding) | ALA 113 | ||
1fj3A02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
1fjoA02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
1fjqA02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
1fjtA02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
1fjuA02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
1fjvA02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
1fjwA02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
1gxwA02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
1hytA02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
1keiA02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
1kjoA02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
1kjpA02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
1kkkA02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
1kl6A02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
1kr6A02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
1kroA02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
1ks7A02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
1ktoA02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
1l3fE02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
1lnaE02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
1lnbE02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
1lncE02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
1lndE02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
1lneE02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
1lnfE02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
1os0A02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
1qf0A02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
1qf1A02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
1qf2A02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
1thlA02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
1tliA02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
1tlpE02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
1tlxA02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
1tmnE02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
1trlA |
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|||||
1trlB |
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2tliA02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
2tlxA02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
2tmnE02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
3tliA02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
3tlnA02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
3tmnE02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
4tliA02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
4tlnA02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
4tmnE02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
5tliA02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
5tlnA02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
5tmnE02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
6tliA02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
6tmnE02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
7tliA02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
7tlnA02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
8tliA02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) | |||
8tlnE02 |
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TYR 157;HIS 231 | GLU 166(Zinc binding) |
References for Catalytic Mechanism | ||
---|---|---|
References | Sections | No. of steps in catalysis |
[3]
|
Fig.5, p.6917-6920 | |
[5]
|
||
[6]
|
p.5727-5728 | |
[7]
|
||
[8]
|
Fig.1, p.8547-8549 | |
[9]
|
||
[10]
|
p.3259 | |
[17]
|
Fig.1, p.16807-16808 | |
[20]
|
Scheme 1, p.7373 | |
[42]
|
Fig.2, Fig.3, p.287-296 |
References | |
---|---|
[1] | |
Resource | |
Comments | ACTIVE SITE. |
Medline ID | 74052951 |
PubMed ID | 4808703 |
Journal | Biochemistry |
Year | 1974 |
Volume | 13 |
Pages | 205-10 |
Authors | Burstein Y, Walsh KA, Neurath H |
Title | Evidence of an essential histidine residue in thermolysin |
Related PDB | |
Related UniProtKB | P00800 |
[2] | |
Resource | |
Comments | X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS). |
Medline ID | 75041142 |
PubMed ID | 4214815 |
Journal | J Biol Chem |
Year | 1974 |
Volume | 249 |
Pages | 8030-44 |
Authors | Matthews BW, Weaver LH, Kester WR |
Title | The conformation of thermolysin |
Related PDB | |
Related UniProtKB | P00800 |
[3] | |
Resource | |
Comments | X-ray crystallography |
Medline ID | |
PubMed ID | 7317361 |
Journal | Biochemistry |
Year | 1981 |
Volume | 20 |
Pages | 6912-20 |
Authors | Holmes MA, Matthews BW |
Title | Binding of hydroxamic acid inhibitors to crystalline thermolysin suggests a pentacoordinate zinc intermediate in catalysis |
Related PDB | 4tln 5tln |
Related UniProtKB | |
[4] | |
Resource | |
Comments | X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS). |
Medline ID | 83085812 |
PubMed ID | 7175940 |
Journal | J Mol Biol |
Year | 1982 |
Volume | 160 |
Pages | 623-39 |
Authors | Holmes MA, Matthews BW |
Title | Structure of thermolysin refined at 1.6 A resolution |
Related PDB | |
Related UniProtKB | P00800 |
[5] | |
Resource | |
Comments | X-ray crystallography |
Medline ID | |
PubMed ID | 6830761 |
Journal | Biochemistry |
Year | 1983 |
Volume | 22 |
Pages | 236-40 |
Authors | Holmes MA, Tronrud DE, Matthews BW |
Title | Structural analysis of the inhibition of thermolysin by an active-site-directed irreversible inhibitor |
Related PDB | 7tln |
Related UniProtKB | |
[6] | |
Resource | |
Comments | X-ray crystallography |
Medline ID | |
PubMed ID | 6395881 |
Journal | Biochemistry |
Year | 1984 |
Volume | 23 |
Pages | 5724-9 |
Authors | Monzingo AF, Matthews BW |
Title | Binding of N-carboxymethyl dipeptide inhibitors to thermolysin determined by X-ray crystallography: a novel class of transition-state analogues for zinc peptidases |
Related PDB | 1tmn |
Related UniProtKB | |
[7] | |
Resource | |
Comments | X-ray crystallography |
Medline ID | |
PubMed ID | 3709536 |
Journal | Eur J Biochem |
Year | 1986 |
Volume | 157 |
Pages | 261-8 |
Authors | Tronrud DE, Monzingo AF, Matthews BW |
Title | Crystallographic structural analysis of phosphoramidates as inhibitors and transition-state analogs of thermolysin |
Related PDB | 1tlp 2tmn |
Related UniProtKB | |
[8] | |
Resource | |
Comments | X-ray crystallography |
Medline ID | |
PubMed ID | 3442675 |
Journal | Biochemistry |
Year | 1987 |
Volume | 26 |
Pages | 8542-53 |
Authors | Holden HM, Tronrud DE, Monzingo AF, Weaver LH, Matthews BW |
Title | Slow- and fast-binding inhibitors of thermolysin display different modes of binding: crystallographic analysis of extended phosphonamidate transition-state analogues |
Related PDB | 4tmn 5tmn |
Related UniProtKB | |
[9] | |
Resource | |
Comments | X-ray crystallography |
Medline ID | |
PubMed ID | 3810156 |
Journal | Science |
Year | 1987 |
Volume | 235 |
Pages | 571-4 |
Authors | Tronrud DE, Holden HM, Matthews BW |
Title | Structures of two thermolysin-inhibitor complexes that differ by a single hydrogen bond |
Related PDB | 6tmn |
Related UniProtKB | |
[10] | |
Resource | |
Comments | X-ray crystallography |
Medline ID | |
PubMed ID | 3343246 |
Journal | J Biol Chem |
Year | 1988 |
Volume | 263 |
Pages | 3256-60 |
Authors | Holden HM, Matthews BW |
Title | The binding of L-valyl-L-tryptophan to crystalline thermolysin illustrates the mode of interaction of a product of peptide hydrolysis |
Related PDB | 3tmn |
Related UniProtKB | |
[11] | |
Resource | |
Comments | |
Medline ID | |
PubMed ID | 2019475 |
Journal | Int J Pept Protein Res |
Year | 1991 |
Volume | 37 |
Pages | 128-33 |
Authors | Miranda MT, Tominaga M |
Title | Thermolysin as a catalyst in enzymatic synthesis of asparagine-containing peptides II |
Related PDB | |
Related UniProtKB | |
[12] | |
Resource | |
Comments | X-ray crystallography |
Medline ID | |
PubMed ID | 1445869 |
Journal | Biochemistry |
Year | 1992 |
Volume | 31 |
Pages | 11310-6 |
Authors | Holland DR, Tronrud DE, Pley HW, Flaherty KM, Stark W, Jansonius JN, McKay DB, Matthews BW |
Title | Structural comparison suggests that thermolysin and related neutral proteases undergo hinge-bending motion during catalysis |
Related PDB | 3tln 8tln |
Related UniProtKB | |
[13] | |
Resource | |
Comments | |
Medline ID | |
PubMed ID | 8286362 |
Journal | Biochemistry |
Year | 1994 |
Volume | 33 |
Pages | 51-6 |
Authors | Holland DR, Barclay PL, Danilewicz JC, Matthews BW, James K |
Title | Inhibition of thermolysin and neutral endopeptidase 24.11 by a novel glutaramide derivative: X-ray structure determination of the thermolysin-inhibitor complex |
Related PDB | 1thl |
Related UniProtKB | |
[14] | |
Resource | |
Comments | STRUCTURE BY NMR OF 487-548. |
Medline ID | 95086079 |
PubMed ID | 7993910 |
Journal | Biochemistry |
Year | 1994 |
Volume | 33 |
Pages | 14834-47 |
Authors | Rico M, Jimenez MA, Gonzalez C, De Filippis V, Fontana A |
Title | NMR solution structure of the C-terminal fragment 255-316 of thermolysin: a dimer formed by subunits having the native structure |
Related PDB | 1trl |
Related UniProtKB | P00800 |
[15] | |
Resource | |
Comments | X-ray crystallography |
Medline ID | |
PubMed ID | 8034637 |
Journal | J Biol Chem |
Year | 1994 |
Volume | 269 |
Pages | 18839-42 |
Authors | Hausrath AC, Matthews BW |
Title | Redetermination and refinement of the complex of benzylsuccinic acid with thermolysin and its relation to the complex with carboxypeptidase A |
Related PDB | 1hyt |
Related UniProtKB | |
[16] | |
Resource | |
Comments | |
Medline ID | |
PubMed ID | 7720870 |
Journal | FEBS Lett |
Year | 1995 |
Volume | 362 |
Pages | 189-91 |
Authors | Kunugi S, Yokoyama M, Sakamoto A |
Title | Observation of the pre-steady state process in thermolysin catalysis with a fluorescent displacement probe at low pH |
Related PDB | |
Related UniProtKB | |
[17] | |
Resource | |
Comments | |
Medline ID | |
PubMed ID | 7622493 |
Journal | J Biol Chem |
Year | 1995 |
Volume | 270 |
Pages | 16803-8 |
Authors | Beaumont A, O'Donohue MJ, Paredes N, Rousselet N, Assicot M, Bohuon C, Fournie-Zaluski MC, Roques BP |
Title |
The role of histidine 231 in thermolysin-like enzymes. |
Related PDB | |
Related UniProtKB | |
[18] | |
Resource | |
Comments | |
Medline ID | |
PubMed ID | 7629812 |
Journal | J Med Chem |
Year | 1995 |
Volume | 38 |
Pages | 2742-7 |
Authors | Beusen DD, McDowell LM, Slomczynska U, Schaefer J |
Title | Solid-state nuclear magnetic resonance analysis of the conformation of an inhibitor bound to thermolysin |
Related PDB | |
Related UniProtKB | |
[19] | |
Resource | |
Comments | X-ray crystallography |
Medline ID | |
PubMed ID | 8535232 |
Journal | Protein Sci |
Year | 1995 |
Volume | 4 |
Pages | 1955-65 |
Authors | Holland DR, Hausrath AC, Juers D, Matthews BW |
Title | Structural analysis of zinc substitutions in the active site of thermolysin |
Related PDB | 1lna 1lnb 1lnc 1lnd 1lne 1lnf |
Related UniProtKB | |
[20] | |
Resource | |
Comments | |
Medline ID | |
PubMed ID | 8652513 |
Journal | Biochemistry |
Year | 1996 |
Volume | 35 |
Pages | 7369-77 |
Authors | Mock WL, Stanford DJ |
Title |
Arazoformyl dipeptide substrates for thermolysin. |
Related PDB | |
Related UniProtKB | |
[21] | |
Resource | |
Comments | STRUCTURE BY NMR OF 437-548. |
Medline ID | 97452621 |
PubMed ID | 9305992 |
Journal | Biochemistry |
Year | 1997 |
Volume | 36 |
Pages | 11975-83 |
Authors | Conejero-Lara F, Gonzalez C, Jimenez MA, Padmanabhan S, Mateo PL, Rico M |
Title |
NMR solution structure of the 205-316 C-terminal fragment of thermolysin. |
Related PDB | |
Related UniProtKB | P00800 |
[22] | |
Resource | |
Comments | |
Medline ID | |
PubMed ID | 9262642 |
Journal | J Pept Sci |
Year | 1997 |
Volume | 3 |
Pages | 245-51 |
Authors | Eichhorn U, Bommarius AS, Drauz K, Jakubke HD |
Title | Synthesis of dipeptides by suspension-to-suspension conversion via thermolysin catalysis: from analytical to preparative scale |
Related PDB | |
Related UniProtKB | |
[23] | |
Resource | |
Comments | |
Medline ID | |
PubMed ID | 9153087 |
Journal | Protein Eng |
Year | 1997 |
Volume | 10 |
Pages | 223-30 |
Authors | Frigerio F, Margarit I, Nogarotto R, Grandi G, Vriend G, Hardy F, Veltman OR, Venema G, Eijsink VG |
Title | Model building of a thermolysin-like protease by mutagenesis. |
Related PDB | |
Related UniProtKB | |
[24] | |
Resource | |
Comments | |
Medline ID | |
PubMed ID | 9928125 |
Journal | Ann N Y Acad Sci |
Year | 1998 |
Volume | 864 |
Pages | 458-62 |
Authors | Pauthe E, Dauchez M, Berry H, Berjot M, Monti JP, Alix AJ, Larreta-Garde V |
Title | Enzymatic and structural approaches of the thermolysin mechanism in glycerol-containing media |
Related PDB | |
Related UniProtKB | |
[25] | |
Resource | |
Comments | |
Medline ID | |
PubMed ID | 10099304 |
Journal | Biotechnol Bioeng |
Year | 1998 |
Volume | 58 |
Pages | 654-7 |
Authors | Bedell BA, Mozhaev VV, Clark DS, Dordick JS |
Title | Testing for diffusion limitations in salt-activated enzyme catalysts operating in organic solvents |
Related PDB | |
Related UniProtKB | |
[26] | |
Resource | |
Comments | |
Medline ID | |
PubMed ID | 9488701 |
Journal | J Biol Chem |
Year | 1998 |
Volume | 273 |
Pages | 5697-701 |
Authors | Marie-Claire C, Roques BP, Beaumont A |
Title | Intramolecular processing of prothermolysin |
Related PDB | |
Related UniProtKB | |
[27] | |
Resource | |
Comments | |
Medline ID | |
PubMed ID | 9735298 |
Journal | J Mol Biol |
Year | 1998 |
Volume | 282 |
Pages | 435-46 |
Authors | Tate S, Ohno A, Seeram SS, Hiraga K, Oda K, Kainosho M |
Title |
Elucidation of the mode of interaction of thermolysin with a proteinaceous metalloproteinase inhibitor, |
Related PDB | |
Related UniProtKB | |
[28] | |
Resource | |
Comments | X-ray crystallography |
Medline ID | |
PubMed ID | 10504225 |
Journal | Biochemistry |
Year | 1999 |
Volume | 38 |
Pages | 12569-76 |
Authors | Gaucher JF, Selkti M, Tiraboschi G, Prange T, Roques BP, Tomas A, Fournie-Zaluski MC |
Title | Crystal structures of alpha-mercaptoacyldipeptides in the thermolysin active site: structural parameters for a Zn monodentation or bidentation in metalloendopeptidases |
Related PDB | 1qf0 1qf1 1qf2 |
Related UniProtKB | |
[29] | |
Resource | |
Comments | |
Medline ID | |
PubMed ID | 10467116 |
Journal | Biotechnol Appl Biochem |
Year | 1999 |
Volume | 30 |
Pages | 35-40 |
Authors | Van den Burg B, de Kreij A, Van der Veek P, Mansfeld J, Venema G |
Title | Characterization of a novel stable biocatalyst obtained by protein engineering. |
Related PDB | |
Related UniProtKB | |
[30] | |
Resource | |
Comments | X-ray crystallography |
Medline ID | |
PubMed ID | 10651278 |
Journal | Proteins |
Year | 1999 |
Volume | 37 |
Pages | 628-40 |
Authors | English AC, Done SH, Caves LS, Groom CR, Hubbard RE |
Title | Locating interaction sites on proteins: the crystal structure of thermolysin soaked in 2% to 100% isopropanol |
Related PDB | 1tli 1tlx 2tlx 2tli 3tli 4tli 5tli 6tli 7tli 8tli |
Related UniProtKB | |
[31] | |
Resource | |
Comments | |
Medline ID | |
PubMed ID | 10869357 |
Journal | J Biol Chem |
Year | 2000 |
Volume | 275 |
Pages | 31115-20 |
Authors | de Kreij A, Venema G, van den Burg B |
Title | Substrate specificity in the highly heterogeneous M4 peptidase family is determined by a small subset of amino acids. |
Related PDB | |
Related UniProtKB | |
[32] | |
Resource | |
Comments | |
Medline ID | |
PubMed ID | 10921773 |
Journal | J Comput Aided Mol Des |
Year | 2000 |
Volume | 14 |
Pages | 573-91 |
Authors | Cosgrove DA, Bayada DM, Johnson AP |
Title | A novel method of aligning molecules by local surface shape similarity |
Related PDB | |
Related UniProtKB | |
[33] | |
Resource | |
Comments | |
Medline ID | |
PubMed ID | 11326120 |
Journal | Acta Crystallogr A |
Year | 2001 |
Volume | 57 |
Pages | 351-8 |
Authors | Webster G, Hilgenfeld R |
Title | An evolutionary computational approach to the phase problem in macromolecular X-ray crystallography |
Related PDB | |
Related UniProtKB | |
[34] | |
Resource | |
Comments | |
Medline ID | |
PubMed ID | 11427942 |
Journal | Biotechnol Bioeng |
Year | 2001 |
Volume | 74 |
Pages | 406-15 |
Authors | Murakami Y, Chiba K, Oda T, Hirata A |
Title | Novel kinetic analysis of enzymatic dipeptide synthesis: effect of pH and substrates on thermolysin catalysis |
Related PDB | |
Related UniProtKB | |
[35] | |
Resource | |
Comments | X-ray crystallography |
Medline ID | |
PubMed ID | 11287678 |
Journal | Protein Eng |
Year | 2001 |
Volume | 14 |
Pages | 47-59 |
Authors | English AC, Groom CR, Hubbard RE |
Title | Experimental and computational mapping of the binding surface of a crystalline protein |
Related PDB | 1fj3 1fjo 1fjq 1fjt 1fju 1fjv 1fjw |
Related UniProtKB | |
[36] | |
Resource | |
Comments | X-ray crystallography |
Medline ID | |
PubMed ID | 12454500 |
Journal | Acta Crystallogr D Biol Crystallogr |
Year | 2002 |
Volume | 58 |
Pages | 2198-200 |
Authors | Gaucher JF, Selkti M, Prange T, Tomas A |
Title | The 2.2 A resolution structure of thermolysin (TLN) crystallized in the presence of potassium thiocyanate. |
Related PDB | 1gxw |
Related UniProtKB | |
[37] | |
Resource | |
Comments | X-ray crystallography |
Medline ID | |
PubMed ID | 12037302 |
Journal | Acta Crystallogr D Biol Crystallogr |
Year | 2002 |
Volume | 58 |
Pages | 1002-7 |
Authors | Hausrath AC, Matthews BW |
Title | Thermolysin in the absence of substrate has an open conformation |
Related PDB | 1l3f |
Related UniProtKB | |
[38] | |
Resource | |
Comments | |
Medline ID | |
PubMed ID | 12023041 |
Journal | FEBS Lett |
Year | 2002 |
Volume | 519 |
Pages | 181-4 |
Authors | Pedersen NR, Halling PJ, Pedersen LH, Wimmer R, Matthiesen R, Veltman OR |
Title | Efficient transesterification of sucrose catalysed by the metalloprotease thermolysin in dimethylsulfoxide |
Related PDB | |
Related UniProtKB | |
[39] | |
Resource | |
Comments | |
Medline ID | |
PubMed ID | 11996551 |
Journal | J Am Chem Soc |
Year | 2002 |
Volume | 124 |
Pages | 5254-5 |
Authors | Rich JO, Mozhaev VV, Dordick JS, Clark DS, Khmelnitsky YL |
Title | Molecular imprinting of enzymes with water-insoluble ligands for nonaqueous biocatalysis |
Related PDB | |
Related UniProtKB | |
[40] | |
Resource | |
Comments | |
Medline ID | |
PubMed ID | 11859085 |
Journal | J Biol Chem |
Year | 2002 |
Volume | 277 |
Pages | 15432-8 |
Authors | de Kreij A, van den Burg B, Venema G, Vriend G, Eijsink VG, Nielsen JE |
Title | The effects of modifying the surface charge on the catalytic activity of a thermolysin-like protease. |
Related PDB | |
Related UniProtKB | |
[41] | |
Resource | |
Comments | |
Medline ID | |
PubMed ID | 11935352 |
Journal | J Biol Inorg Chem |
Year | 2002 |
Volume | 7 |
Pages | 284-98 |
Authors | Pelmenschikov V, Blomberg MR, Siegbahn PE |
Title | A theoretical study of the mechanism for peptide hydrolysis by thermolysin. |
Related PDB | |
Related UniProtKB | |
[42] | |
Resource | |
Comments | |
Medline ID | |
PubMed ID | 11858637 |
Journal | J Mol Graph Model |
Year | 2002 |
Volume | 20 |
Pages | 281-95 |
Authors | Clark RD, Strizhev A, Leonard JM, Blake JF, Matthew JB |
Title | Consensus scoring for ligand/protein interactions |
Related PDB | |
Related UniProtKB | |
[43] | |
Resource | |
Comments | X-ray crystallography |
Medline ID | |
PubMed ID | 12832763 |
Journal | Acta Crystallogr D Biol Crystallogr |
Year | 2003 |
Volume | 59 |
Pages | 1200-5 |
Authors | Selkti M, Tomas A, Gaucher JF, Prange T, Fournie-Zaluski MC, Chen H, Roques BP |
Title | Interactions of a new alpha-aminophosphinic derivative inside the active site of TLN (thermolysin): a model for zinc-metalloendopeptidase inhibition. |
Related PDB | 1os0 |
Related UniProtKB | |
[44] | |
Resource | |
Comments | |
Medline ID | |
PubMed ID | 12569506 |
Journal | Angew Chem Int Ed Engl |
Year | 2003 |
Volume | 42 |
Pages | 421-4 |
Authors | Kelso MJ, Hoang HN, Oliver W, Sokolenko N, March DR, Appleton TG, Fairlie DP |
Title | A cyclic metallopeptide induces alpha helicity in short peptide fragments of thermolysin. |
Related PDB | |
Related UniProtKB | |
[45] | |
Resource | |
Comments | |
Medline ID | |
PubMed ID | 12735988 |
Journal | Bioorg Med Chem |
Year | 2003 |
Volume | 11 |
Pages | 2421-6 |
Authors | Kim SJ, Kim DH, Park JD, Woo JR, Jin Y, Ryu SE |
Title | Origin of the stereospecificity in binding hydroxamates of alpha- and beta-phenylalanine methylamide to thermolysin revealed by the X-ray crystallographic study. |
Related PDB | |
Related UniProtKB |
Comments |
---|
This enzyme belongs to peptidase family-M4 (Thermolysin family).
Although this enzyme binds four calcium ions, According to the literature [8], (1) Carbonyl oxygen of Target bond and hydrorytic water molecule are bound to zinc ion, (2) The water molecule makes a nucleophilic attack on the carbonyl carbon to form a tetrahedral intermediate. (3) The intermediate is stabilized by His231, (4) Glu143 acts as a genral acid to protonate the leaving amine group. |
Created | Updated |
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2005-04-01 | 2009-03-16 |