RLCP 1.13.10900.463 :EzCatDB EzCatDB

R L C P
Hierarchic Classification of Catalytic Mechanisms


R 1.-.-.- : Hydrolysis (bond cleavage by water) (Reaction)

L 1.13.-.- : Amide bond (One of Carbonyl Ester bonds; including peptide bond) (Ligand group involved)

C 1.13.10900.- : Thermolysin-like mechanism (Acid-Base/Stabilizer); Inorganic cofactor-assisted water activated by base; Activated water attacks on target bond; Inorganic cofactor-assisted stabilization of intermediate (bond & leaving group); Protonation to leaving group by acid leading to cleavage of bond; SN1/SN2 unclear (Catalytic mechanism)

P 1.13.10900.463 : Glu + His/Tyr/Asn + mainchain carbonyl + zinc ion bound to His/Glu cluster (Residues/cofactors in Protein)

1st Nucleophile : water
Catalytic groups : cofactor + groups in residue/substrate
General Base : a catalytic residue
General Acid : a catalytic residue

Related Enzymes

There are 4 entries in this class.
  • D00233 : 3.4.24.26; Pseudolysin (Catalytic domains; 1.10.390.10, 3.10.170.10)
  • D00234 : 3.4.24.27; Thermolysin (Catalytic domains; 1.10.390.10, 3.10.170.10)
  • D00235 : 3.4.24.28; Bacillolysin (Catalytic domains; 1.10.390.10, 3.10.170.10)
  • D00480 : 3.4.24.29; Zinc metalloproteinase aureolysin (Catalytic domains; 1.10.390.10, 3.10.170.10)
    • Copyright: Nozomi Nagano, JST & CBRC-AIST
    Funded by PRESTO/Japan Science and Technology Agency (JST) (December 2001 - November 2004)
    Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2005 - March 2006)
    Funded by Grant-in-Aid for Scientific Research (B)/Japan Society for the Promotion of Science (JSPS) (April 2005 - March 2008)
    Funded by BIRD/Japan Science and Technology Agency (JST) (September 2005 - September 2008)
    Funded by BIRD/Japan Science and Technology Agency (JST) (October 2007 - September 2010)
    Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2011 - March 2012)
    Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2012 - March 2013)
    Supported by the commission for the Development of Artificial Gene Synthesis Technology for Creating Innovative Biomaterial from the Ministry of Economy, Trade and Industry (METI) (October 2012 - March 2016)
    Funded by the project commissioned by the New Energy and Industrial Technology Development Organization (NEDO) (April 2016 -)