DB code: S00227
| CATH domain | 3.20.20.100 : TIM Barrel | Catalytic domain |
|---|---|---|
| E.C. | 1.1.1.2 | |
| CSA | 2alr | |
| M-CSA | 2alr | |
| MACiE | ||
| CATH domain | Related DB codes (homologues) |
|---|---|
| 3.20.20.100 : TIM Barrel | S00228 S00229 |
| Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | RefSeq | Pfam |
|---|---|---|---|---|
| P14550 |
Alcohol dehydrogenase {NADP+}
|
EC
1.1.1.2
Aldehyde reductase Aldo-keto reductase family 1 member A1 |
NP_001189342.1
(Protein)
NM_001202413.1 (DNA/RNA sequence) NP_001189343.1 (Protein) NM_001202414.1 (DNA/RNA sequence) NP_006057.1 (Protein) NM_006066.3 (DNA/RNA sequence) NP_697021.1 (Protein) NM_153326.2 (DNA/RNA sequence) |
PF00248
(Aldo_ket_red)
[Graphical View] |
| KEGG enzyme name |
|---|
|
alcohol dehydrogenase (NADP+)
aldehyde reductase (NADPH2) NADP-alcohol dehydrogenase NADP+-aldehyde reductase NADP+-dependent aldehyde reductase NADPH-aldehyde reductase NADPH-dependent aldehyde reductase nonspecific succinic semialdehyde reductase ALR 1 low-Km aldehyde reductase high-Km aldehyde reductase alcohol dehydrogenase (NADP+) |
| UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
|---|---|---|---|---|---|
| P14550 | AK1A1_HUMAN | An alcohol + NADP(+) = an aldehyde + NADPH. | Monomer (By similarity). |
| KEGG Pathways | Map code | Pathways | E.C. |
|---|---|---|
| MAP00010 | Glycolysis / Gluconeogenesis | |
| MAP00561 | Glycerolipid metabolism | |
| MAP00930 | Caprolactam degradation |
| Compound table | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Substrates | Products | Intermediates | |||||||||
| KEGG-id | C00006 | C00226 | C00432 | C00005 | C00071 | ||||||
| E.C. | |||||||||||
| Compound | NADP+ | Primary alcohol | Secondary alcohol | NADPH | Aldehyde | ||||||
| Type | amide group,amine group,nucleotide | carbohydrate | carbohydrate | amide group,amine group,nucleotide | carbohydrate | ||||||
| ChEBI |
18009 18009 |
16474 16474 |
|||||||||
| PubChem |
5886 5886 |
5884 5884 |
|||||||||
| 2alrA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1hqtA |
|
|
|
|
|
Bound:NAP | Unbound | Unbound | Unbound | Unbound | |
| Reference for Active-site residues | ||
|---|---|---|
| resource | references | E.C. |
| see S00228 | ||
| Active-site residues | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| PDB | Catalytic residues | Cofactor-binding residues | Modified residues | Main-chain involved in catalysis | Comment | |||||
| 2alrA |
|
|
|
|
|
ASP 44;TYR 49;LYS 79;HIS 112 | ||||
| 1hqtA |
|
|
|
|
|
ASP 45; ;LYS 80;HIS 113 | mutant Y50F | |||
| References for Catalytic Mechanism | ||
|---|---|---|
| References | Sections | No. of steps in catalysis |
|
[2]
|
p.866-868 | |
|
[3]
|
Fig.3 | |
| References | |
|---|---|
| [1] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 3160340 |
| Journal | Biochem J |
| Year | 1985 |
| Volume | 228 |
| Pages | 363-73 |
| Authors | Esterbauer H, Zollner H, Lang J |
| Title | Metabolism of the lipid peroxidation product 4-hydroxynonenal by isolated hepatocytes and by liver cytosolic fractions. |
| Related PDB | |
| Related UniProtKB | |
| [2] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.48 ANGSTROMS) |
| Medline ID | |
| PubMed ID | |
| Journal | Acta Crystallogr D Biol Crystallogr |
| Year | 1994 |
| Volume | 50 |
| Pages | 859-68 |
| Authors | El-Kabbani O, Green NC, Lin G, Carson M, Narayana SVL., Moore KM, Flynn TG, DeLucas LJ |
| Title | Structures of human and porcine aldehyde reductase: an enzyme implicated in diabetic complications |
| Related PDB | 2alr |
| Related UniProtKB | P14550 |
| [3] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9521768 |
| Journal | Biochemistry |
| Year | 1998 |
| Volume | 37 |
| Pages | 4482-9 |
| Authors | Cho H, Plapp BV |
| Title | Specificity of alcohol dehydrogenases for sulfoxides. |
| Related PDB | |
| Related UniProtKB | |
| [4] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10694410 |
| Journal | Biochemistry |
| Year | 2000 |
| Volume | 39 |
| Pages | 2406-12 |
| Authors | Northrop DB, Cho YK |
| Title | Effect of pressure on deuterium isotope effects of yeast alcohol dehydrogenase: evidence for mechanical models of catalysis. |
| Related PDB | |
| Related UniProtKB | |
| [5] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10651037 |
| Journal | Proteins |
| Year | 2000 |
| Volume | 38 |
| Pages | 41-8 |
| Authors | Ye Q, Hyndman D, Li X, Flynn TG, Jia Z |
| Title |
Crystal structure of CHO reductase, |
| Related PDB | 1c9w |
| Related UniProtKB | |
| [6] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11279029 |
| Journal | J Biol Chem |
| Year | 2001 |
| Volume | 276 |
| Pages | 19253-8 |
| Authors | Tryggvason K, Romert A, Eriksson U |
| Title |
Biosynthesis of 9-cis-retinoic acid in vivo. |
| Related PDB | |
| Related UniProtKB | |
| [7] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11306083 |
| Journal | Chem Biol Interact |
| Year | 2001 |
| Volume | 130-132 |
| Pages | 651-8 |
| Authors | Ye Q, Hyndman D, Green NC, Li L, Jia Z, Flynn TG |
| Title | The crystal structure of an aldehyde reductase Y50F mutant-NADP complex and its implications for substrate binding. |
| Related PDB | 1hqt |
| Related UniProtKB | |
| Comments |
|---|
|
This enzyme is homologous to aldose reductase (S00228 in EzCatDB), |
| Created | Updated |
|---|---|
| 2004-02-02 | 2009-09-28 |