DB code: M00180
| RLCP classification | 9.5010.584100.976 : Hydride transfer | |
|---|---|---|
| 5.501.400060.67 : Elimination | ||
| 9.5010.536000.976 : Hydride transfer | ||
| CATH domain | -.-.-.- : | |
| 1.10.3270.10 : HMGR, N-terminal domain | ||
| 3.90.770.10 : 3-hydroxy-3-methylglutaryl-coenzyme A Reductase; Chain A, domain 2 | Catalytic domain | |
| 3.30.70.420 : Alpha-Beta Plaits | Catalytic domain | |
| E.C. | 1.1.1.34 | |
| CSA | 1dqa | |
| M-CSA | 1dqa | |
| MACiE | M0093 | |
| CATH domain | Related DB codes (homologues) |
|---|---|
| 3.30.70.420 : Alpha-Beta Plaits | D00607 |
| 3.90.770.10 : 3-hydroxy-3-methylglutaryl-coenzyme A Reductase; Chain A, domain 2 | D00607 |
| Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | RefSeq | Pfam |
|---|---|---|---|---|
| P04035 |
3-hydroxy-3-methylglutaryl-coenzyme A reductase
|
HMG-CoA reductase
EC 1.1.1.34 |
NP_000850.1
(Protein)
NM_000859.2 (DNA/RNA sequence) NP_001124468.1 (Protein) NM_001130996.1 (DNA/RNA sequence) |
PF00368
(HMG-CoA_red)
[Graphical View] |
| KEGG enzyme name |
|---|
|
hydroxymethylglutaryl-CoA reductase (NADPH)
hydroxymethylglutaryl coenzyme A reductase (reduced nicotinamideadenine dinucleotide phosphate) 3-hydroxy-3-methylglutaryl-CoA reductase beta-hydroxy-beta-methylglutaryl coenzyme A reductase hydroxymethylglutaryl CoA reductase (NADPH) S-3-hydroxy-3-methylglutaryl-CoA reductase NADPH-hydroxymethylglutaryl-CoA reductase HMGCoA reductase-mevalonate:NADP-oxidoreductase (acetylating-CoA) 3-hydroxy-3-methylglutaryl CoA reductase (NADPH) hydroxymethylglutaryl-CoA reductase (NADPH2) |
| UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
|---|---|---|---|---|---|
| P04035 | HMDH_HUMAN | (R)-mevalonate + CoA + 2 NADP(+) = (S)-3- hydroxy-3-methylglutaryl-CoA + 2 NADPH. | Homodimer. | Endoplasmic reticulum membrane, Multi-pass membrane protein. Peroxisome membrane, Multi-pass membrane protein. |
| KEGG Pathways | Map code | Pathways | E.C. |
|---|---|---|
| MAP00100 | Biosynthesis of steroids |
| Compound table | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Substrates | Products | Intermediates | |||||||||||
| KEGG-id | C00356 | C00005 | C00080 | C00418 | C00010 | C00006 | I00101 | I00102 | |||||
| E.C. | |||||||||||||
| Compound | (S)-3-Hydroxy-3-methylglutaryl-CoA | NADPH | H+ | (R)-Mevalonate | CoA | NADP+ | Mevaldyl-CoA | Mevaldehyde | |||||
| Type | amine group,carbohydrate,carboxyl group,nucleotide ,peptide/protein,sulfide group | amide group,amine group,nucleotide | others | carbohydrate,carboxyl group | amine group,carbohydrate,nucleotide ,peptide/protein,sulfhydryl group | amide group,amine group,nucleotide | |||||||
| ChEBI |
15467 15467 |
16474 16474 |
15378 15378 |
17710 17710 |
15346 15346 |
18009 18009 |
|||||||
| PubChem |
439218 445127 439218 445127 |
5884 5884 |
1038 1038 |
439230 439230 |
6816 87642 6816 87642 |
5886 5886 |
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| 1dq8A01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1dq8B01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1dq8C01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1dq8D01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1dq9A01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1dq9B01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1dq9C01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1dq9D01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1dqaA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1dqaB01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1dqaC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1dqaD01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1hw8A01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1hw8B01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1hw8C01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1hw8D01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1hw9A01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1hw9B01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1hw9C01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1hw9D01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1hwiA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1hwiB01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1hwiC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1hwiD01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1hwjA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1hwjB01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1hwjC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1hwjD01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1hwkA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1hwkB01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1hwkC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1hwkD01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1hwlA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1hwlB01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1hwlC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1hwlD01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1dq8A02 |
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Unbound | Unbound | Bound:COA | Unbound | Unbound | Unbound | ||
| 1dq8B02 |
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Unbound | Unbound | Bound:COA | Unbound | Unbound | Intermediate-analogue:MAH | ||
| 1dq8C02 |
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Unbound | Unbound | Bound:COA | Unbound | Unbound | Intermediate-analogue:MAH | ||
| 1dq8D02 |
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Unbound | Unbound | Bound:COA | Unbound | Unbound | Intermediate-analogue:MAH | ||
| 1dq9A02 |
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Bound:HMG | Unbound | Unbound | Unbound | Unbound | Unbound | Intermediate-analogue:MAH | |
| 1dq9B02 |
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Bound:HMG | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1dq9C02 |
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Bound:HMG | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1dq9D02 |
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Bound:HMG | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1dqaA02 |
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Unbound | Unbound | Bound:COA | Unbound | Unbound | Unbound | ||
| 1dqaB02 |
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Unbound | Unbound | Bound:COA | Unbound | Unbound | Intermediate-analogue:MAH | ||
| 1dqaC02 |
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Unbound | Unbound | Bound:COA | Unbound | Unbound | Intermediate-analogue:MAH | ||
| 1dqaD02 |
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Unbound | Unbound | Bound:COA | Unbound | Unbound | Intermediate-analogue:MAH | ||
| 1hw8A02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Intermediate-analogue:114 | Intermediate-analogue:MAH | |
| 1hw8B02 |
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Unbound | Unbound | Unbound | Analogue:ADP | Unbound | Intermediate-analogue:114 | Unbound | |
| 1hw8C02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Intermediate-analogue:114 | Unbound | |
| 1hw8D02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Intermediate-analogue:114 | Unbound | |
| 1hw9A02 |
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Unbound | Unbound | Unbound | Analogue:ADP | Unbound | Intermediate-analogue:SIM | Unbound | |
| 1hw9B02 |
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Unbound | Unbound | Unbound | Analogue:ADP | Unbound | Intermediate-analogue:SIM | Unbound | |
| 1hw9C02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Intermediate-analogue:SIM | Unbound | |
| 1hw9D02 |
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Unbound | Unbound | Unbound | Analogue:ADP | Unbound | Intermediate-analogue:SIM | Unbound | |
| 1hwiA02 |
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Unbound | Unbound | Unbound | Analogue:ADP | Unbound | Intermediate-analogue:115 | Unbound | |
| 1hwiB02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Intermediate-analogue:115 | Unbound | |
| 1hwiC02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Intermediate-analogue:115 | Unbound | |
| 1hwiD02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Intermediate-analogue:115 | Unbound | |
| 1hwjA02 |
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Unbound | Unbound | Unbound | Analogue:ADP | Unbound | Intermediate-analogue:116 | Unbound | |
| 1hwjB02 |
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Unbound | Unbound | Unbound | Analogue:ADP | Unbound | Intermediate-analogue:116 | Unbound | |
| 1hwjC02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Intermediate-analogue:116 | Unbound | |
| 1hwjD02 |
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Unbound | Unbound | Unbound | Analogue:ADP | Unbound | Intermediate-analogue:116 | Unbound | |
| 1hwkA02 |
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Unbound | Unbound | Unbound | Analogue:ADP | Unbound | Intermediate-analogue:117 | Unbound | |
| 1hwkB02 |
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Unbound | Unbound | Unbound | Analogue:ADP | Unbound | Intermediate-analogue:117 | Unbound | |
| 1hwkC02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Intermediate-analogue:117 | Unbound | |
| 1hwkD02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Intermediate-analogue:117 | Unbound | |
| 1hwlA02 |
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Unbound | Unbound | Unbound | Analogue:ADP | Unbound | Intermediate-analogue:FBI | Unbound | |
| 1hwlB02 |
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Unbound | Unbound | Unbound | Analogue:ADP | Unbound | Intermediate-analogue:FBI | Unbound | |
| 1hwlC02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Intermediate-analogue:FBI | Unbound | |
| 1hwlD02 |
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Unbound | Unbound | Unbound | Analogue:ADP | Unbound | Intermediate-analogue:FBI | Unbound | |
| 1dq8A03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1dq8B03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1dq8C03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1dq8D03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1dq9A03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1dq9B03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1dq9C03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1dq9D03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1dqaA03 |
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Unbound | Unbound | Unbound | Unbound | Bound:NAP | Unbound | Unbound | |
| 1dqaB03 |
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Unbound | Unbound | Unbound | Unbound | Bound:NAP | Unbound | Unbound | |
| 1dqaC03 |
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Unbound | Unbound | Unbound | Unbound | Bound:NAP | Unbound | Unbound | |
| 1dqaD03 |
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Unbound | Unbound | Unbound | Unbound | Bound:NAP | Unbound | Unbound | |
| 1hw8A03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1hw8B03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1hw8C03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1hw8D03 |
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Unbound | Unbound | Unbound | Unbound | Analogue:ADP | Unbound | Unbound | |
| 1hw9A03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1hw9B03 |
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Unbound | Unbound | Unbound | Unbound | Analogue:ADP | Unbound | Unbound | |
| 1hw9C03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1hw9D03 |
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Unbound | Unbound | Unbound | Unbound | Analogue:ADP | Unbound | Unbound | |
| 1hwiA03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1hwiB03 |
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Unbound | Unbound | Unbound | Unbound | Analogue:ADP | Unbound | Unbound | |
| 1hwiC03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1hwiD03 |
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Unbound | Unbound | Unbound | Unbound | Analogue:ADP | Unbound | Unbound | |
| 1hwjA03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1hwjB03 |
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Unbound | Unbound | Unbound | Unbound | Analogue:ADP | Unbound | Unbound | |
| 1hwjC03 |
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Unbound | Unbound | Unbound | Unbound | Analogue:ADP | Unbound | Unbound | |
| 1hwjD03 |
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Unbound | Unbound | Unbound | Unbound | Analogue:ADP | Unbound | Unbound | |
| 1hwkA03 |
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Unbound | Unbound | Unbound | Unbound | Analogue:ADP | Unbound | Unbound | |
| 1hwkB03 |
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Unbound | Unbound | Unbound | Unbound | Analogue:ADP | Unbound | Unbound | |
| 1hwkC03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1hwkD03 |
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Unbound | Unbound | Unbound | Unbound | Analogue:ADP | Unbound | Unbound | |
| 1hwlA03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1hwlB03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1hwlC03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1hwlD03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| Reference for Active-site residues | ||
|---|---|---|
| resource | references | E.C. |
| Swiss-prot;P04035 & literature [19] | ||
| Active-site residues | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| PDB | Catalytic residues | Cofactor-binding residues | Modified residues | Main-chain involved in catalysis | Comment | |||||
| 1dq8A01 |
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mutant M485I | ||||
| 1dq8B01 |
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mutant M485I | ||||
| 1dq8C01 |
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mutant M485I | ||||
| 1dq8D01 |
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mutant M485I | ||||
| 1dq9A01 |
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mutant M485I | ||||
| 1dq9B01 |
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mutant M485I | ||||
| 1dq9C01 |
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mutant M485I | ||||
| 1dq9D01 |
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mutant M485I | ||||
| 1dqaA01 |
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mutant M485I | ||||
| 1dqaB01 |
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mutant M485I | ||||
| 1dqaC01 |
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mutant M485I | ||||
| 1dqaD01 |
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mutant M485I | ||||
| 1hw8A01 |
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mutant M485I | ||||
| 1hw8B01 |
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mutant M485I | ||||
| 1hw8C01 |
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mutant M485I | ||||
| 1hw8D01 |
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mutant M485I | ||||
| 1hw9A01 |
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mutant M485I | ||||
| 1hw9B01 |
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mutant M485I | ||||
| 1hw9C01 |
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mutant M485I | ||||
| 1hw9D01 |
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mutant M485I | ||||
| 1hwiA01 |
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mutant M485I | ||||
| 1hwiB01 |
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mutant M485I | ||||
| 1hwiC01 |
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mutant M485I | ||||
| 1hwiD01 |
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mutant M485I | ||||
| 1hwjA01 |
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mutant M485I | ||||
| 1hwjB01 |
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mutant M485I | ||||
| 1hwjC01 |
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mutant M485I | ||||
| 1hwjD01 |
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mutant M485I | ||||
| 1hwkA01 |
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mutant M485I | ||||
| 1hwkB01 |
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mutant M485I | ||||
| 1hwkC01 |
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mutant M485I | ||||
| 1hwkD01 |
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mutant M485I | ||||
| 1hwlA01 |
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mutant M485I | ||||
| 1hwlB01 |
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mutant M485I | ||||
| 1hwlC01 |
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mutant M485I | ||||
| 1hwlD01 |
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mutant M485I | ||||
| 1dq8A02 |
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GLU 559;ASP 767; | invisible H866 | |||
| 1dq8B02 |
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GLU 559;ASP 767; | invisible H866 | |||
| 1dq8C02 |
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GLU 559;ASP 767; | invisible H866 | |||
| 1dq8D02 |
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GLU 559;ASP 767; | invisible H866 | |||
| 1dq9A02 |
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GLU 559;ASP 767; | invisible H866 | |||
| 1dq9B02 |
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GLU 559;ASP 767; | invisible H866 | |||
| 1dq9C02 |
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GLU 559;ASP 767;HIS 866 | ||||
| 1dq9D02 |
|
|
|
|
|
GLU 559;ASP 767; | invisible H866 | |||
| 1dqaA02 |
|
|
|
|
|
GLU 559;ASP 767;HIS 866 | ||||
| 1dqaB02 |
|
|
|
|
|
GLU 559;ASP 767;HIS 866 | ||||
| 1dqaC02 |
|
|
|
|
|
GLU 559;ASP 767;HIS 866 | ||||
| 1dqaD02 |
|
|
|
|
|
GLU 559;ASP 767;HIS 866 | ||||
| 1hw8A02 |
|
|
|
|
|
GLU 559;ASP 767; | invisible H866 | |||
| 1hw8B02 |
|
|
|
|
|
GLU 559;ASP 767; | invisible H866 | |||
| 1hw8C02 |
|
|
|
|
|
GLU 559;ASP 767; | invisible H866 | |||
| 1hw8D02 |
|
|
|
|
|
GLU 559;ASP 767; | invisible H866 | |||
| 1hw9A02 |
|
|
|
|
|
GLU 559;ASP 767; | invisible H866 | |||
| 1hw9B02 |
|
|
|
|
|
GLU 559;ASP 767; | invisible H866 | |||
| 1hw9C02 |
|
|
|
|
|
GLU 559;ASP 767; | invisible H866 | |||
| 1hw9D02 |
|
|
|
|
|
GLU 559;ASP 767; | invisible H866 | |||
| 1hwiA02 |
|
|
|
|
|
GLU 559;ASP 767; | invisible H866 | |||
| 1hwiB02 |
|
|
|
|
|
GLU 559;ASP 767; | invisible H866 | |||
| 1hwiC02 |
|
|
|
|
|
GLU 559;ASP 767; | invisible H866 | |||
| 1hwiD02 |
|
|
|
|
|
GLU 559;ASP 767; | invisible H866 | |||
| 1hwjA02 |
|
|
|
|
|
GLU 559;ASP 767; | invisible H866 | |||
| 1hwjB02 |
|
|
|
|
|
GLU 559;ASP 767; | invisible H866 | |||
| 1hwjC02 |
|
|
|
|
|
GLU 559;ASP 767; | invisible H866 | |||
| 1hwjD02 |
|
|
|
|
|
GLU 559;ASP 767; | invisible H866 | |||
| 1hwkA02 |
|
|
|
|
|
GLU 559;ASP 767; | invisible H866 | |||
| 1hwkB02 |
|
|
|
|
|
GLU 559;ASP 767; | invisible H866 | |||
| 1hwkC02 |
|
|
|
|
|
GLU 559;ASP 767; | invisible H866 | |||
| 1hwkD02 |
|
|
|
|
|
GLU 559;ASP 767; | invisible H866 | |||
| 1hwlA02 |
|
|
|
|
|
GLU 559;ASP 767; | invisible H866 | |||
| 1hwlB02 |
|
|
|
|
|
GLU 559;ASP 767; | invisible H866 | |||
| 1hwlC02 |
|
|
|
|
|
GLU 559;ASP 767; | invisible H866 | |||
| 1hwlD02 |
|
|
|
|
|
GLU 559;ASP 767; | invisible H866 | |||
| 1dq8A03 |
|
|
|
|
|
LYS 691 | ||||
| 1dq8B03 |
|
|
|
|
|
LYS 691 | ||||
| 1dq8C03 |
|
|
|
|
|
LYS 691 | ||||
| 1dq8D03 |
|
|
|
|
|
LYS 691 | ||||
| 1dq9A03 |
|
|
|
|
|
LYS 691 | ||||
| 1dq9B03 |
|
|
|
|
|
LYS 691 | ||||
| 1dq9C03 |
|
|
|
|
|
LYS 691 | ||||
| 1dq9D03 |
|
|
|
|
|
LYS 691 | ||||
| 1dqaA03 |
|
|
|
|
|
LYS 691 | ||||
| 1dqaB03 |
|
|
|
|
|
LYS 691 | ||||
| 1dqaC03 |
|
|
|
|
|
LYS 691 | ||||
| 1dqaD03 |
|
|
|
|
|
LYS 691 | ||||
| 1hw8A03 |
|
|
|
|
|
LYS 691 | ||||
| 1hw8B03 |
|
|
|
|
|
LYS 691 | ||||
| 1hw8C03 |
|
|
|
|
|
LYS 691 | ||||
| 1hw8D03 |
|
|
|
|
|
LYS 691 | ||||
| 1hw9A03 |
|
|
|
|
|
LYS 691 | ||||
| 1hw9B03 |
|
|
|
|
|
LYS 691 | ||||
| 1hw9C03 |
|
|
|
|
|
LYS 691 | ||||
| 1hw9D03 |
|
|
|
|
|
LYS 691 | ||||
| 1hwiA03 |
|
|
|
|
|
LYS 691 | ||||
| 1hwiB03 |
|
|
|
|
|
LYS 691 | ||||
| 1hwiC03 |
|
|
|
|
|
LYS 691 | ||||
| 1hwiD03 |
|
|
|
|
|
LYS 691 | ||||
| 1hwjA03 |
|
|
|
|
|
LYS 691 | ||||
| 1hwjB03 |
|
|
|
|
|
LYS 691 | ||||
| 1hwjC03 |
|
|
|
|
|
LYS 691 | ||||
| 1hwjD03 |
|
|
|
|
|
LYS 691 | ||||
| 1hwkA03 |
|
|
|
|
|
LYS 691 | ||||
| 1hwkB03 |
|
|
|
|
|
LYS 691 | ||||
| 1hwkC03 |
|
|
|
|
|
LYS 691 | ||||
| 1hwkD03 |
|
|
|
|
|
LYS 691 | ||||
| 1hwlA03 |
|
|
|
|
|
LYS 691 | ||||
| 1hwlB03 |
|
|
|
|
|
LYS 691 | ||||
| 1hwlC03 |
|
|
|
|
|
LYS 691 | ||||
| 1hwlD03 |
|
|
|
|
|
LYS 691 | ||||
| References for Catalytic Mechanism | ||
|---|---|---|
| References | Sections | No. of steps in catalysis |
|
[8]
|
Fig.1 | |
|
[13]
|
Fig.7, p.11481-11482 | |
|
[15]
|
Fig.8, p.16865-16866 | |
|
[17]
|
Scheme 1, Scheme 2 | |
|
[19]
|
Fig. 1, Fig. 5, p.14-16 | |
|
[20]
|
p.826-827 | |
| References | |
|---|---|
| [1] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 6247408 |
| Journal | J Lipid Res |
| Year | 1980 |
| Volume | 21 |
| Pages | 399-405 |
| Authors | Hunter CF, Rodwell VW |
| Title | Regulation of vertebrate liver HMG-CoA reductase via reversible modulation of its catalytic activity. |
| Related PDB | |
| Related UniProtKB | |
| [2] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2991281 |
| Journal | J Biol Chem |
| Year | 1985 |
| Volume | 260 |
| Pages | 10271-7 |
| Authors | Luskey KL, Stevens B |
| Title |
Human 3-hydroxy-3-methylglutaryl coenzyme A reductase. |
| Related PDB | |
| Related UniProtKB | |
| [3] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 3058035 |
| Journal | Arch Biochem Biophys |
| Year | 1988 |
| Volume | 267 |
| Pages | 110-8 |
| Authors | Mayer RJ, Debouck C, Metcalf BW |
| Title | Purification and properties of the catalytic domain of human 3-hydroxy-3-methylglutaryl-CoA reductase expressed in Escherichia coli. |
| Related PDB | |
| Related UniProtKB | |
| [4] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 3065625 |
| Journal | Mol Cell Biol |
| Year | 1988 |
| Volume | 8 |
| Pages | 3797-808 |
| Authors | Basson ME, Thorsness M, Finer-Moore J, Stroud RM, Rine J |
| Title |
Structural and functional conservation between yeast and human 3-hydroxy-3-methylglutaryl coenzyme A reductases, |
| Related PDB | |
| Related UniProtKB | |
| [5] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2491679 |
| Journal | Plant Mol Biol |
| Year | 1989 |
| Volume | 13 |
| Pages | 627-38 |
| Authors | Caelles C, Ferrer A, Balcells L, Hegardt FG, Boronat A |
| Title | Isolation and structural characterization of a cDNA encoding Arabidopsis thaliana 3-hydroxy-3-methylglutaryl coenzyme A reductase. |
| Related PDB | |
| Related UniProtKB | |
| [6] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2813388 |
| Journal | Proc Natl Acad Sci U S A |
| Year | 1989 |
| Volume | 86 |
| Pages | 8217-21 |
| Authors | Rajkovic A, Simonsen JN, Davis RE, Rottman FM |
| Title | Molecular cloning and sequence analysis of 3-hydroxy-3-methylglutaryl-coenzyme A reductase from the human parasite Schistosoma mansoni. |
| Related PDB | |
| Related UniProtKB | |
| [7] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2369897 |
| Journal | EMBO J |
| Year | 1990 |
| Volume | 9 |
| Pages | 2439-46 |
| Authors | Clarke PR, Hardie DG |
| Title | Regulation of HMG-CoA reductase: identification of the site phosphorylated by the AMP-activated protein kinase in vitro and in intact rat liver. |
| Related PDB | |
| Related UniProtKB | |
| [8] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2123872 |
| Journal | J Biol Chem |
| Year | 1990 |
| Volume | 265 |
| Pages | 21634-41 |
| Authors | Wang Y, Darnay BG, Rodwell VW |
| Title | Identification of the principal catalytically important acidic residue of 3-hydroxy-3-methylglutaryl coenzyme A reductase. |
| Related PDB | |
| Related UniProtKB | |
| [9] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1685759 |
| Journal | Lipids |
| Year | 1991 |
| Volume | 26 |
| Pages | 637-48 |
| Authors | Bach TJ, Boronat A, Caelles C, Ferrer A, Weber T, Wettstein A |
| Title | Aspects related to mevalonate biosynthesis in plants. |
| Related PDB | |
| Related UniProtKB | |
| [10] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8374303 |
| Journal | Protein Expr Purif |
| Year | 1993 |
| Volume | 4 |
| Pages | 337-44 |
| Authors | Frimpong K, Darnay BG, Rodwell VW |
| Title | Syrian hamster 3-hydroxy-3-methylglutaryl-coenzyme A reductase expressed in Escherichia coli: production of homogeneous protein. |
| Related PDB | |
| Related UniProtKB | |
| [11] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8112324 |
| Journal | Eur J Biochem |
| Year | 1994 |
| Volume | 219 |
| Pages | 743-50 |
| Authors | Ball KL, Dale S, Weekes J, Hardie DG |
| Title | Biochemical characterization of two forms of 3-hydroxy-3-methylglutaryl-CoA reductase kinase from cauliflower (Brassica oleracia). |
| Related PDB | |
| Related UniProtKB | |
| [12] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8288583 |
| Journal | J Biol Chem |
| Year | 1994 |
| Volume | 269 |
| Pages | 1217-21 |
| Authors | Frimpong K, Rodwell VW |
| Title | The active site of hamster 3-hydroxy-3-methylglutaryl-CoA reductase resides at the subunit interface and incorporates catalytically essential acidic residues from separate polypeptides. |
| Related PDB | |
| Related UniProtKB | |
| [13] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7908908 |
| Journal | J Biol Chem |
| Year | 1994 |
| Volume | 269 |
| Pages | 11478-83 |
| Authors | Frimpong K, Rodwell VW |
| Title |
Catalysis by Syrian hamster 3-hydroxy-3-methylglutaryl-coenzyme A reductase. |
| Related PDB | |
| Related UniProtKB | |
| [14] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8120043 |
| Journal | J Biol Chem |
| Year | 1994 |
| Volume | 269 |
| Pages | 6810-4 |
| Authors | Omkumar RV, Darnay BG, Rodwell VW |
| Title |
Modulation of Syrian hamster 3-hydroxy-3-methylglutaryl-CoA reductase activity by phosphorylation. |
| Related PDB | |
| Related UniProtKB | |
| [15] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8207009 |
| Journal | J Biol Chem |
| Year | 1994 |
| Volume | 269 |
| Pages | 16862-6 |
| Authors | Omkumar RV, Rodwell VW |
| Title | Phosphorylation of Ser871 impairs the function of His865 of Syrian hamster 3-hydroxy-3-methylglutaryl-CoA reductase. |
| Related PDB | |
| Related UniProtKB | |
| [16] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8302869 |
| Journal | Proc Natl Acad Sci U S A |
| Year | 1994 |
| Volume | 91 |
| Pages | 927-31 |
| Authors | Enjuto M, Balcells L, Campos N, Caelles C, Arro M, Boronat A |
| Title |
Arabidopsis thaliana contains two differentially expressed 3-hydroxy-3-methylglutaryl-CoA reductase genes, |
| Related PDB | |
| Related UniProtKB | |
| [17] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10413460 |
| Journal | Biochemistry |
| Year | 1999 |
| Volume | 38 |
| Pages | 8879-83 |
| Authors | Bochar DA, Tabernero L, Stauffacher CV, Rodwell VW |
| Title | Aminoethylcysteine can replace the function of the essential active site lysine of Pseudomonas mevalonii 3-hydroxy-3-methylglutaryl coenzyme A reductase. |
| Related PDB | |
| Related UniProtKB | |
| [18] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9973353 |
| Journal | J Bacteriol |
| Year | 1999 |
| Volume | 181 |
| Pages | 1256-63 |
| Authors | Takahashi S, Kuzuyama T, Seto H |
| Title |
Purification, |
| Related PDB | |
| Related UniProtKB | |
| [19] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11111074 |
| Journal | Biochim Biophys Acta |
| Year | 2000 |
| Volume | 1529 |
| Pages | 9-18 |
| Authors | Istvan ES, Deisenhofer J |
| Title | The structure of the catalytic portion of human HMG-CoA reductase. |
| Related PDB | |
| Related UniProtKB | |
| [20] | |
| Resource | |
| Comments |
X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 422-888, |
| Medline ID | |
| PubMed ID | 10698924 |
| Journal | EMBO J |
| Year | 2000 |
| Volume | 19 |
| Pages | 819-30 |
| Authors | Istvan ES, Palnitkar M, Buchanan SK, Deisenhofer J |
| Title | Crystal structure of the catalytic portion of human HMG-CoA reductase: insights into regulation of activity and catalysis. |
| Related PDB | 1dq8 1dq9 1dqa 1dq8 1dq9 |
| Related UniProtKB | |
| [21] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11370859 |
| Journal | Mol Genet Genomics |
| Year | 2001 |
| Volume | 265 |
| Pages | 135-42 |
| Authors | Kato-Emori S, Higashi K, Hosoya K, Kobayashi T, Ezura H |
| Title |
Cloning and characterization of the gene encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase in melon (Cucumis melo L. |
| Related PDB | |
| Related UniProtKB | |
| [22] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 11349148 |
| Journal | Science |
| Year | 2001 |
| Volume | 292 |
| Pages | 1160-4 |
| Authors | Istvan ES, Deisenhofer J |
| Title | Structural mechanism for statin inhibition of HMG-CoA reductase. |
| Related PDB | 1hw8 1hw9 1hwi 1hwj 1hwk 1hwl |
| Related UniProtKB | |
| Comments |
|---|
|
This enzyme is composed of the N-terminal transmembrane domain, HMG-CoA reductase from Pseudomonas mevalonii, According to the literature [13], (A) Hydride transfer from NADPH to HMG-CoA, (A0) Asp767 may modulate the charge/activity of Glu559 and Lys691. (A1) Hydride transfer occurs from nicotinamide group of NADPH to the carbonyl carbon of the substrate HMG-CoA. (B) Eliminative double-bond formation; Elimination of CoASH from Mevaldyl-CoA, (B0) Asp767 may modulate the charge/activity of Glu559 and Lys691. (B1) Glu559 and Lys691 stabilizes the negative charge on the oxygen atom of the intermediate. (B2) His866 on the C-terminal movable domain acts as general acid to protonate the sulfur atom (or thioanion) of the eliminated group, (C) Hydride transfer from NADPH to Mevaldehyde, (C0) Asp767 may modulate the charge/activity of Glu559 and Lys691. (C1) Lys691 acts as a general acid to protonate the carbonyl oxygen of Mevaldehyde. |
| Created | Updated |
|---|---|
| 2004-03-24 | 2011-09-05 |