DB code: D00094
| CATH domain | 1.10.166.10 : Tetrahydrodipicolinate-N-succinyltransferase; Chain A, domain 1 | |
|---|---|---|
| 2.160.10.10 : UDP N-Acetylglucosamine Acyltransferase; domain 1 | Catalytic domain | |
| E.C. | 2.3.1.117 | |
| CSA | 2tdt | |
| M-CSA | 2tdt | |
| MACiE | ||
| CATH domain | Related DB codes (homologues) |
|---|---|
| 2.160.10.10 : UDP N-Acetylglucosamine Acyltransferase; domain 1 | D00464 S00167 D00417 |
| Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | Pfam |
|---|---|---|---|
| P56220 |
2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase
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EC
2.3.1.117
Tetrahydrodipicolinate N-succinyltransferase THP succinyltransferase Tetrahydropicolinate succinylase |
PF00132
(Hexapep)
[Graphical View] |
| KEGG enzyme name |
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2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase
tetrahydropicolinate succinylase tetrahydrodipicolinate N-succinyltransferase tetrahydrodipicolinate succinyltransferase succinyl-CoA:tetrahydrodipicolinate N-succinyltransferase succinyl-CoA:2,3,4,5-tetrahydropyridine-2,6-dicarboxylateN-succinyltransferase |
| UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
|---|---|---|---|---|---|
| P56220 | DAPD_MYCBO | Succinyl-CoA + (S)-2,3,4,5-tetrahydropyridine- 2,6-dicarboxylate + H(2)O = CoA + N-succinyl-L-2-amino-6- oxoheptanedioate. | Homotrimer. | Cytoplasm. |
| KEGG Pathways | Map code | Pathways | E.C. |
|---|---|---|
| MAP00300 | Lysine biosynthesis |
| Compound table | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Substrates | Products | Intermediates | |||||||||
| KEGG-id | C00091 | C03972 | C00001 | C00010 | C04462 | ||||||
| E.C. | |||||||||||
| Compound | Succinyl-CoA | 2,3,4,5-Tetrahydrodipicolinate | H2O | CoA | N-Succinyl-L-2-amino-6-oxoheptanedioate | ||||||
| Type | amine group,carbohydrate,carboxyl group,nucleotide ,peptide/protein,sulfide group | carboxyl group,imine group | H2O | amine group,carbohydrate,nucleotide ,peptide/protein,sulfhydryl group | amino acids,amide group,carbohydrate,carboxyl group | ||||||
| ChEBI |
15380 15380 |
864 864 |
15377 15377 |
15346 15346 |
35266 35266 |
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| PubChem |
439161 92133 439161 92133 |
440179 440179 |
22247451 962 22247451 962 |
6816 87642 6816 87642 |
440349 440349 |
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| 1tdtA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1tdtB01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1tdtC01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2tdtA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 3tdtA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1tdtA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1tdtB02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1tdtC02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2tdtA02 |
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Unbound | Unbound | Bound:COA | Unbound | Intermediate-analogue:NPI | |
| 3tdtA02 |
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Unbound | Unbound | Bound:COA | Unbound | Bound:26P | |
| Reference for Active-site residues | ||
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| resource | references | E.C. |
| literature [3] | ||
| Active-site residues | ||||||||||
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| PDB | Catalytic residues | Cofactor-binding residues | Modified residues | Main-chain involved in catalysis | Comment | |||||
| 1tdtA01 |
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| 1tdtB01 |
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| 1tdtC01 |
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| 2tdtA01 |
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| 3tdtA01 |
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| 1tdtA02 |
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ASP 141 | ||||
| 1tdtB02 |
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ASP 141 | ||||
| 1tdtC02 |
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ASP 141 | ||||
| 2tdtA02 |
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ASP 141 | ||||
| 3tdtA02 |
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ASP 141 | ||||
| References for Catalytic Mechanism | ||
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| References | Sections | No. of steps in catalysis |
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[3]
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p.10368-10369 | |
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[5]
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p.975-978 | |
| References | |
|---|---|
| [1] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8880935 |
| Journal | Proteins |
| Year | 1996 |
| Volume | 26 |
| Pages | 115-7 |
| Authors | Binder DA, Blanchard JS, Roderick SL |
| Title | Crystallization and preliminary crystallographic analysis of tetrahydrodipicolinate-N-succinyltransferase. |
| Related PDB | |
| Related UniProtKB | |
| [2] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) |
| Medline ID | 97164902 |
| PubMed ID | 9012664 |
| Journal | Biochemistry |
| Year | 1997 |
| Volume | 36 |
| Pages | 489-94 |
| Authors | Beaman TW, Binder DA, Blanchard JS, Roderick SL |
| Title | Three-dimensional structure of tetrahydrodipicolinate N-succinyltransferase. |
| Related PDB | 1tdt |
| Related UniProtKB | P56220 |
| [3] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) |
| Medline ID | 98337777 |
| PubMed ID | 9671504 |
| Journal | Biochemistry |
| Year | 1998 |
| Volume | 37 |
| Pages | 10363-9 |
| Authors | Beaman TW, Blanchard JS, Roderick SL |
| Title | The conformational change and active site structure of tetrahydrodipicolinate N-succinyltransferase. |
| Related PDB | 2tdt 3tdt |
| Related UniProtKB | P56220 |
| [4] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11106178 |
| Journal | Protein Sci |
| Year | 2000 |
| Volume | 9 |
| Pages | 2034-7 |
| Authors | Cirilli M, Scapin G, Sutherland A, Vederas JC, Blanchard JS |
| Title | The three-dimensional structure of the ternary complex of Corynebacterium glutamicum diaminopimelate dehydrogenase-NADPH-L-2-amino-6-methylene-pimelate. |
| Related PDB | |
| Related UniProtKB | |
| [5] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11910040 |
| Journal | Protein Sci |
| Year | 2002 |
| Volume | 11 |
| Pages | 974-9 |
| Authors | Beaman TW, Vogel KW, Drueckhammer DG, Blanchard JS, Roderick SL |
| Title | Acyl group specificity at the active site of tetrahydridipicolinate N-succinyltransferase. |
| Related PDB | |
| Related UniProtKB | |
| Comments |
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| Created | Updated |
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| 2004-03-17 | 2009-02-26 |