DB code: T00255
| CATH domain | 1.20.890.10 : cAMP-dependent Protein Kinase, Chain A | |
|---|---|---|
| 2.60.120.10 : Jelly Rolls | ||
| 2.60.120.10 : Jelly Rolls | ||
| E.C. | 2.7.11.11 | |
| CSA | ||
| M-CSA | ||
| MACiE | ||
| CATH domain | Related DB codes (homologues) |
|---|---|
| 2.60.120.10 : Jelly Rolls | S00145 S00155 D00842 D00843 M00216 T00101 |
| Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | Contains | RefSeq | Pfam |
|---|---|---|---|---|---|
| P05132 |
cAMP-dependent protein kinase catalytic subunit alpha
|
PKA C-alpha
EC 2.7.11.11 |
None |
NP_001264827.1
(Protein)
NM_001277898.1 (DNA/RNA sequence) NP_032880.1 (Protein) NM_008854.5 (DNA/RNA sequence) |
PF00069
(Pkinase)
[Graphical View] |
| P00517 |
cAMP-dependent protein kinase catalytic subunit alpha
|
PKA C-alpha
EC 2.7.11.11 |
None |
NP_777009.1
(Protein)
NM_174584.2 (DNA/RNA sequence) |
PF00069
(Pkinase)
[Graphical View] |
| Q9DBC7 |
cAMP-dependent protein kinase type I-alpha regulatory subunit
|
None |
cAMP-dependent protein kinase type I-alpha regulatory subunit, N-terminally processed
|
NP_068680.1
(Protein)
NM_021880.2 (DNA/RNA sequence) |
PF00027
(cNMP_binding)
PF02197 (RIIa) [Graphical View] |
| P12849 |
cAMP-dependent protein kinase type I-beta regulatory subunit
|
None | None |
NP_001240819.1
(Protein)
NM_001253890.1 (DNA/RNA sequence) NP_032949.3 (Protein) NM_008923.3 (DNA/RNA sequence) |
PF00027
(cNMP_binding)
PF02197 (RIIa) [Graphical View] |
| P12367 |
cAMP-dependent protein kinase type II-alpha regulatory subunit
|
None | None |
PF00027
(cNMP_binding)
PF02197 (RIIa) [Graphical View] |
|
| P31324 |
cAMP-dependent protein kinase type II-beta regulatory subunit
|
None | None |
NP_035288.2
(Protein)
NM_011158.3 (DNA/RNA sequence) |
PF00027
(cNMP_binding)
PF02197 (RIIa) [Graphical View] |
| P00514 |
cAMP-dependent protein kinase type I-alpha regulatory subunit
|
None |
cAMP-dependent protein kinase type I-alpha regulatory subunit, N-terminally processed
|
NP_001069826.1
(Protein)
NM_001076358.1 (DNA/RNA sequence) |
PF00027
(cNMP_binding)
PF02197 (RIIa) [Graphical View] |
| P00515 |
cAMP-dependent protein kinase type II-alpha regulatory subunit
|
None | None |
NP_001178296.1
(Protein)
NM_001191367.1 (DNA/RNA sequence) |
PF00027
(cNMP_binding)
PF02197 (RIIa) [Graphical View] |
| P31322 |
cAMP-dependent protein kinase type II-beta regulatory subunit
|
None | None |
NP_777074.1
(Protein)
NM_174649.2 (DNA/RNA sequence) |
PF00027
(cNMP_binding)
PF02197 (RIIa) [Graphical View] |
| KEGG enzyme name |
|---|
|
cAMP-dependent protein kinase
PKA PKA C protein kinase A STK22 |
| UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
|---|---|---|---|---|---|
| P05132 | KAPCA_MOUSE | ATP + a protein = ADP + a phosphoprotein. | A number of inactive tetrameric holoenzymes are produced by the combination of homo- or heterodimers of the different regulatory subunits associated with two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. | Cytoplasm (By similarity). Nucleus (By similarity). Note=Translocates into the nucleus (monomeric catalytic subunit) (By similarity). The inactive holoenzyme is found in the cytoplasm (By similarity). | |
| P00517 | KAPCA_BOVIN | ATP + a protein = ADP + a phosphoprotein. | A number of inactive tetrameric holoenzymes are produced by the combination of homo- or heterodimers of the different regulatory subunits associated with two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. | Cytoplasm. Nucleus. Note=Translocates into the nucleus (monomeric catalytic subunit). The inactive holoenzyme is found in the cytoplasm. | |
| Q9DBC7 | KAP0_MOUSE | The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. PRKAR1A also interacts with RFC2, the complex may be involved in cell survival (By similarity). Interacts with AKAP4. | |||
| P12849 | KAP1_MOUSE | The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. | |||
| P12367 | KAP2_MOUSE | The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Interacts with AKAP4. Interacts with CBFA2T3 (By similarity). | |||
| P31324 | KAP3_MOUSE | The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. | |||
| P00514 | KAP0_BOVIN | The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. PRKAR1A also interacts with RFC2, the complex may be involved in cell survival. Interacts with AKAP4 (By similarity). | |||
| P00515 | KAP2_BOVIN | The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Interacts with AKAP4 and CBFA2T3 (By similarity). | |||
| P31322 | KAP3_BOVIN | The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. |
| KEGG Pathways | Map code | Pathways | E.C. |
|---|
| Compound table | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Substrates | Products | Intermediates | ||||||||
| KEGG-id | C00002 | C00017 | C00008 | C00562 | ||||||
| E.C. | ||||||||||
| Compound | ATP | Protein | ADP | Phosphoprotein | ||||||
| Type | amine group,nucleotide | peptide/protein | amine group,nucleotide | peptide/protein,phosphate group/phosphate ion | ||||||
| ChEBI |
15422 15422 |
16761 16761 |
||||||||
| PubChem |
5957 5957 |
6022 6022 |
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| 1r2aA |
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Unbound | Unbound | Unbound | Unbound | |
| 1r2aB |
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Unbound | Unbound | Unbound | Unbound | |
| 1rgsA01 |
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Unbound | Unbound | Unbound | Unbound | |
| 1rgsA02 |
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Unbound | Unbound | Unbound | Unbound | |
| Reference for Active-site residues | ||
|---|---|---|
| resource | references | E.C. |
| Active-site residues | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| PDB | Catalytic residues | Cofactor-binding residues | Modified residues | Main-chain involved in catalysis | Comment | |||||
| 1r2aA |
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| 1r2aB |
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| 1rgsA01 |
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| 1rgsA02 |
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| References for Catalytic Mechanism | ||
|---|---|---|
| References | Sections | No. of steps in catalysis |
| References | |
|---|---|
| [1] | |
| Resource | |
| Comments | DISULFIDE BONDS |
| Medline ID | 88033069 |
| PubMed ID | 3667618 |
| Journal | J Biol Chem |
| Year | 1987 |
| Volume | 262 |
| Pages | 14961-6 |
| Authors | Bubis J, Vedvick TS, Taylor SS |
| Title | Antiparallel alignment of the two protomers of the regulatory subunit dimer of cAMP-dependent protein kinase I. |
| Related PDB | |
| Related UniProtKB | P00514 |
| [2] | |
| Resource | |
| Comments | 3D-STRUCTURE MODELING |
| Medline ID | 87157645 |
| PubMed ID | 3030405 |
| Journal | Biochemistry |
| Year | 1987 |
| Volume | 26 |
| Pages | 343-51 |
| Authors | Weber IT, Steitz TA, Bubis J, Taylor SS |
| Title | Predicted structures of cAMP binding domains of type I and II regulatory subunits of cAMP-dependent protein kinase. |
| Related PDB | |
| Related UniProtKB | P00514 |
| [3] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 113-379 |
| Medline ID | |
| PubMed ID | 7638597 |
| Journal | Science |
| Year | 1995 |
| Volume | 269 |
| Pages | 807-13 |
| Authors | Su Y, Dostmann WR, Herberg FW, Durick K, Xuong NH, Ten Eyck L, Taylor SS, Varughese KI |
| Title | Regulatory subunit of protein kinase A: structure of deletion mutant with cAMP binding domains. |
| Related PDB | 1rgs |
| Related UniProtKB | P00514 |
| [4] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7873523 |
| Journal | Biochemistry |
| Year | 1995 |
| Volume | 34 |
| Pages | 2447-54 |
| Authors | Adams JA, McGlone ML, Gibson R, Taylor SS |
| Title | Phosphorylation modulates catalytic function and regulation in the cAMP-dependent protein kinase. |
| Related PDB | |
| Related UniProtKB | |
| [5] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7756252 |
| Journal | Biochemistry |
| Year | 1995 |
| Volume | 34 |
| Pages | 6267-71 |
| Authors | Yang S, Fletcher WH, Johnson DA |
| Title | Regulation of cAMP-dependent protein kinase: enzyme activation without dissociation. |
| Related PDB | |
| Related UniProtKB | |
| [6] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8524844 |
| Journal | Proc Natl Acad Sci U S A |
| Year | 1995 |
| Volume | 92 |
| Pages | 11761-5 |
| Authors | Luo K, Zhou P, Lodish HF |
| Title | The specificity of the transforming growth factor beta receptor kinases determined by a spatially addressable peptide library. |
| Related PDB | |
| Related UniProtKB | |
| [7] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8612810 |
| Journal | FEBS Lett |
| Year | 1996 |
| Volume | 384 |
| Pages | 138-42 |
| Authors | Sahara S, Sato K, Kaise H, Mori K, Sato A, Aoto M, Tokmakov AA, Fukami Y |
| Title | Biochemical evidence for the interaction of regulatory subunit of cAMP-dependent protein kinase with IDA (Inter-DFG-APE) region of catalytic subunit. |
| Related PDB | |
| Related UniProtKB | |
| [8] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9300482 |
| Journal | Protein Sci |
| Year | 1997 |
| Volume | 6 |
| Pages | 1825-34 |
| Authors | Gibson RM, Ji-Buechler Y, Taylor SS |
| Title | Identification of electrostatic interaction sites between the regulatory and catalytic subunits of cyclic AMP-dependent protein kinase. |
| Related PDB | |
| Related UniProtKB | |
| [9] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9050991 |
| Journal | Oncogene |
| Year | 1997 |
| Volume | 14 |
| Pages | 923-8 |
| Authors | Tortora G, Damiano V, Bianco C, Baldassarre G, Bianco AR, Lanfrancone L, Pelicci PG, Ciardiello F |
| Title | The RIalpha subunit of protein kinase A (PKA) binds to Grb2 and allows PKA interaction with the activated EGF-receptor. |
| Related PDB | |
| Related UniProtKB | |
| [10] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9295304 |
| Journal | J Biol Chem |
| Year | 1997 |
| Volume | 272 |
| Pages | 23637-44 |
| Authors | Newlon MG, Roy M, Hausken ZE, Scott JD, Jennings PA |
| Title | The A-kinase anchoring domain of type IIalpha cAMP-dependent protein kinase is highly helical. |
| Related PDB | |
| Related UniProtKB | |
| [11] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10485331 |
| Journal | Mol Cell Biochem |
| Year | 1999 |
| Volume | 197 |
| Pages | 117-28 |
| Authors | Nikolakaki E, Fissentzidis A, Giannakouros T, Georgatsos JG |
| Title | Purification and characterization of a dimer form of the cAMP-dependent protein kinase from mouse liver cytosol. |
| Related PDB | |
| Related UniProtKB | |
| [12] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10026146 |
| Journal | J Biol Chem |
| Year | 1999 |
| Volume | 274 |
| Pages | 5370-8 |
| Authors | Zimmermann B, Chiorini JA, Ma Y, Kotin RM, Herberg FW |
| Title | PrKX is a novel catalytic subunit of the cAMP-dependent protein kinase regulated by the regulatory subunit type I. |
| Related PDB | |
| Related UniProtKB | |
| [13] | |
| Resource | |
| Comments | STRUCTURE BY NMR OF 1-43. |
| Medline ID | 99173235 |
| PubMed ID | 10074940 |
| Journal | Nat Struct Biol |
| Year | 1999 |
| Volume | 6 |
| Pages | 222-7 |
| Authors | Newlon MG, Roy M, Morikis D, Hausken ZE, Coghlan V, Scott JD, Jennings PA |
| Title | The molecular basis for protein kinase A anchoring revealed by solution NMR. |
| Related PDB | 1r2a |
| Related UniProtKB | P12367 |
| [14] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11206066 |
| Journal | Protein Sci |
| Year | 2000 |
| Volume | 9 |
| Pages | 2446-56 |
| Authors | Muhonen WW, Shabb JB |
| Title | Resonant mirror biosensor analysis of type Ialpha cAMP-dependent protein kinase B domain--cyclic nucleotide interactions. |
| Related PDB | |
| Related UniProtKB | |
| [15] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10801316 |
| Journal | Biochemistry |
| Year | 2000 |
| Volume | 39 |
| Pages | 5662-71 |
| Authors | Leon DA, Canaves JM, Taylor SS |
| Title | Probing the multidomain structure of the type I regulatory subunit of cAMP-dependent protein kinase using mutational analysis: role and environment of endogenous tryptophans. |
| Related PDB | |
| Related UniProtKB | |
| [16] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10753751 |
| Journal | Curr Biol |
| Year | 2000 |
| Volume | 10 |
| Pages | 417-20 |
| Authors | Diviani D, Langeberg LK, Doxsey SJ, Scott JD |
| Title | Pericentrin anchors protein kinase A at the centrosome through a newly identified RII-binding domain. |
| Related PDB | |
| Related UniProtKB | |
| [17] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10899163 |
| Journal | J Biol Chem |
| Year | 2000 |
| Volume | 275 |
| Pages | 35146-52 |
| Authors | Banky P, Newlon MG, Roy M, Garrod S, Taylor SS, Jennings PA |
| Title | Isoform-specific differences between the type Ialpha and IIalpha cyclic AMP-dependent protein kinase anchoring domains revealed by solution NMR. |
| Related PDB | |
| Related UniProtKB | |
| [18] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11342137 |
| Journal | Structure (Camb) |
| Year | 2001 |
| Volume | 9 |
| Pages | 73-82 |
| Authors | Diller TC, Madhusudan, Xuong NH, Taylor SS |
| Title | Molecular basis for regulatory subunit diversity in cAMP-dependent protein kinase: crystal structure of the type II beta regulatory subunit. |
| Related PDB | |
| Related UniProtKB | |
| [19] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11593431 |
| Journal | Oncogene |
| Year | 2001 |
| Volume | 20 |
| Pages | 6225-32 |
| Authors | Fukuyama T, Sueoka E, Sugio Y, Otsuka T, Niho Y, Akagi K, Kozu T |
| Title | MTG8 proto-oncoprotein interacts with the regulatory subunit of type II cyclic AMP-dependent protein kinase in lymphocytes. |
| Related PDB | |
| Related UniProtKB | |
| [20] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12381327 |
| Journal | J Mol Biol |
| Year | 2002 |
| Volume | 323 |
| Pages | 377-86 |
| Authors | Anand GS, Hughes CA, Jones JM, Taylor SS, Komives EA |
| Title | Amide H/2H exchange reveals communication between the cAMP and catalytic subunit-binding sites in the R(I)alpha subunit of protein kinase A. |
| Related PDB | |
| Related UniProtKB | |
| [21] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11943768 |
| Journal | J Biol Chem |
| Year | 2002 |
| Volume | 277 |
| Pages | 22902-8 |
| Authors | Dahle MK, Gronning LM, Cederberg A, Blomhoff HK, Miura N, Enerback S, Tasken KA, Tasken K |
| Title | Mechanisms of FOXC2- and FOXD1-mediated regulation of the RI alpha subunit of cAMP-dependent protein kinase include release of transcriptional repression and activation by protein kinase B alpha and cAMP. |
| Related PDB | |
| Related UniProtKB | |
| [22] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11877433 |
| Journal | J Biol Chem |
| Year | 2002 |
| Volume | 277 |
| Pages | 16814-22 |
| Authors | Rayner TF, Gray JV, Thorner JW |
| Title |
Direct and novel regulation of cAMP-dependent protein kinase by Mck1p, |
| Related PDB | |
| Related UniProtKB | |
| [23] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11799117 |
| Journal | J Biol Chem |
| Year | 2002 |
| Volume | 277 |
| Pages | 12423-31 |
| Authors | Tung CS, Walsh DA, Trewhella J |
| Title | A structural model of the catalytic subunit-regulatory subunit dimeric complex of the cAMP-dependent protein kinase. |
| Related PDB | |
| Related UniProtKB | |
| Comments |
|---|
|
The E.C. This protein is a regulatory subunit. The C-terminal domains of this protein have got the cAMP binding sites. |
| Created | Updated |
|---|---|
| 2003-07-16 | 2009-03-19 |