DB code: S00145

CATH domain 2.60.120.10 : Jelly Rolls Catalytic domain
E.C. 1.2.3.4
CSA
M-CSA
MACiE

CATH domain Related DB codes (homologues)
2.60.120.10 : Jelly Rolls S00155 D00842 D00843 T00255 M00216 T00101

Uniprot Enzyme Name
UniprotKB Protein name Synonyms Pfam
P45850 Oxalate oxidase 1
EC 1.2.3.4
Germin
PF00190 (Cupin_1)
[Graphical View]

KEGG enzyme name
oxalate oxidase
aero-oxalo dehydrogenase
oxalic acid oxidase

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P45850 OXO1_HORVU Oxalate + O(2) + 2 H(+) = 2 CO(2) + H(2)O(2). Hexamer. Secreted, extracellular space, apoplast (By similarity). Secreted, cell wall (By similarity).

KEGG Pathways
Map code Pathways E.C.
MAP00630 Glyoxylate and dicarboxylate metabolism

Compound table
Cofactors Substrates Products Intermediates
KEGG-id C00034 C00007 C00080 C00209 C00011 C00027
E.C.
Compound Manganese O2 H+ Oxalate CO2 H2O2
Type heavy metal others others carboxyl group others others
ChEBI 18291
 35154
 18291
 35154
 		15379
 26689
 27140
 15379
 26689
 27140
 		15378
 15378
 		16995
 16995
 		16526
 16526
 		16240
 16240
PubChem 23930
 23930
 		977
 977
 		1038
 1038
 		18676629
 3819775
 971
 18676629
 3819775
 971
 		280
 280
 		22326046
 784
 22326046
 784
1fi2A Bound:_MN Unbound Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.
Swiss-prot;P45850

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1fi2A HIS 88;HIS 90;GLU 95;HIS 137(Manganese binding)

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[9]
p.1038-1039
[10]
Fig.8, p.141-144
[11]
Scheme 2, p.177-179

References
[1]
Resource
Comments
Medline ID
PubMed ID 9143327
Journal Arch Biochem Biophys
Year 1997
Volume 340
Pages 239-49
Authors Kotsira VP, Clonis YD
Title Oxalate oxidase from barley roots: purification to homogeneity and study of some molecular, catalytic, and binding properties.
Related PDB
Related UniProtKB
[2]
Resource
Comments
Medline ID
PubMed ID 9349269
Journal Plant Mol Biol
Year 1997
Volume 35
Pages 459-69
Authors Membre N, Berna A, Neutelings G, David A, David H, Staiger D, Saez Vasquez J, Raynal M, Delseny M, Bernier F
Title cDNA sequence, genomic organization and differential expression of three Arabidopsis genes for germin/oxalate oxidase-like proteins.
Related PDB
Related UniProtKB
[3]
Resource
Comments
Medline ID
PubMed ID 9765992
Journal Biochem Soc Trans
Year 1998
Volume 26
Pages S273
Authors Requena L, Bornemann S
Title Structure and function studies of oxalate oxidase.
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID 9573603
Journal Biotechnol Genet Eng Rev
Year 1998
Volume 15
Pages 1-32
Authors Dunwell JM
Title Cupins: a new superfamily of functionally diverse proteins that include germins and plant storage proteins.
Related PDB
Related UniProtKB
[5]
Resource
Comments
Medline ID
PubMed ID 9804177
Journal FEBS Lett
Year 1998
Volume 437
Pages 87-90
Authors Woo EJ, Dunwell JM, Goodenough PW, Pickersgill RW
Title Barley oxalate oxidase is a hexameric protein related to seed storage proteins: evidence from X-ray crystallography.
Related PDB
Related UniProtKB
[6]
Resource
Comments
Medline ID
PubMed ID 9541544
Journal J Mol Evol
Year 1998
Volume 46
Pages 488-93
Authors Gane PJ, Dunwell JM, Warwicker J
Title Modeling based on the structure of vicilins predicts a histidine cluster in the active site of oxalate oxidase.
Related PDB
Related UniProtKB
[7]
Resource
Comments
Medline ID
PubMed ID 10493928
Journal Biochem J
Year 1999
Volume 343 Pt 1
Pages 185-90
Authors Requena L, Bornemann S
Title Barley (Hordeum vulgare) oxalate oxidase is a manganese-containing enzyme.
Related PDB
Related UniProtKB
[8]
Resource
Comments
Medline ID
PubMed ID 10952990
Journal J Biol Chem
Year 2000
Volume 275
Pages 36726-33
Authors Carter C, Thornburg RW
Title Tobacco nectarin I. Purification and characterization as a germin-like, manganese superoxide dismutase implicated in the defensE of floral reproductive tissues.
Related PDB
Related UniProtKB
[9]
Resource
Comments X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS)
Medline ID 20517598
PubMed ID 11062559
Journal Nat Struct Biol
Year 2000
Volume 7
Pages 1036-40
Authors Woo EJ, Dunwell JM, Goodenough PW, Marvier AC, Pickersgill RW
Title Germin is a manganese containing homohexamer with oxalate oxidase and superoxide dismutase activities.
Related PDB 1fi2
Related UniProtKB P45850
[10]
Resource
Comments
Medline ID
PubMed ID 11862550
Journal J Biol Inorg Chem
Year 2002
Volume 7
Pages 136-45
Authors Whittaker MM, Whittaker JW
Title Characterization of recombinant barley oxalate oxidase expressed by Pichia pastoris.
Related PDB
Related UniProtKB
[11]
Resource
Comments
Medline ID
PubMed ID 15581576
Journal Arch Biochem Biophys
Year 2005
Volume 433
Pages 176-92
Authors Svedruzic D, Jonsson S, Toyota CG, Reinhardt LA, Ricagno S, Lindqvist Y, Richards NG
Title The enzymes of oxalate metabolism: unexpected structures and mechanisms.
Related PDB
Related UniProtKB

Comments
This enzyme belongs to the germin family.
The manganese ion seems to play an important role in catalysis.

Created Updated
2004-01-30 2009-03-19