DB code: T00094
| RLCP classification | 5.203.8000.93 : Elimination | |
|---|---|---|
| CATH domain | 1.10.275.10 : Fumarase C; Chain B, domain 1 | |
| 1.20.200.10 : Fumarase C; Chain A, domain 2 | Catalytic domain | |
| 1.10.40.30 : Ribonucleotide Reductase Protein R1; domain 1 | ||
| E.C. | 4.3.2.1 | |
| CSA | 1auw | |
| M-CSA | 1auw | |
| MACiE | ||
| CATH domain | Related DB codes (homologues) |
|---|---|
| 1.10.275.10 : Fumarase C; Chain B, domain 1 | D00267 T00086 T00092 T00095 |
| 1.10.40.30 : Ribonucleotide Reductase Protein R1; domain 1 | T00086 T00092 T00095 |
| 1.20.200.10 : Fumarase C; Chain A, domain 2 | D00267 T00086 T00092 T00095 |
| Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | RefSeq | Pfam |
|---|---|---|---|---|
| P11447 |
Argininosuccinate lyase
|
ASAL
EC 4.3.2.1 Arginosuccinase |
NP_418395.1
(Protein)
NC_000913.2 (DNA/RNA sequence) YP_491492.1 (Protein) NC_007779.1 (DNA/RNA sequence) |
PF00206
(Lyase_1)
[Graphical View] |
| P24058 |
Argininosuccinate lyase
|
ASAL
EC 4.3.2.1 Arginosuccinase Delta crystallin II Delta-2 crystallin |
PF00206
(Lyase_1)
[Graphical View] |
|
| P04424 |
Argininosuccinate lyase
|
ASAL
EC 4.3.2.1 Arginosuccinase |
NP_000039.2
(Protein)
NM_000048.3 (DNA/RNA sequence) NP_001020114.1 (Protein) NM_001024943.1 (DNA/RNA sequence) NP_001020115.1 (Protein) NM_001024944.1 (DNA/RNA sequence) NP_001020117.1 (Protein) NM_001024946.1 (DNA/RNA sequence) |
PF00206
(Lyase_1)
[Graphical View] |
| KEGG enzyme name |
|---|
|
argininosuccinate lyase
arginosuccinase argininosuccinic acid lyase arginine-succinate lyase N-(L-argininosuccinate) arginine-lyase omega-N-(L-arginino)succinate arginine-lyase 2-(omega-N-L-arginino)succinate arginine-lyase (fumarate-forming) |
| UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
|---|---|---|---|---|---|
| P11447 | ARLY_ECOLI | 2-(N(omega)-L-arginino)succinate = fumarate + L-arginine. | Cytoplasm (Probable). | ||
| P24058 | ARLY2_ANAPL | 2-(N(omega)-L-arginino)succinate = fumarate + L-arginine. | Homotetramer. | ||
| P04424 | ARLY_HUMAN | 2-(N(omega)-L-arginino)succinate = fumarate + L-arginine. | Homotetramer. |
| KEGG Pathways | Map code | Pathways | E.C. |
|---|---|---|
| MAP00220 | Urea cycle and metabolism of amino groups | |
| MAP00252 | Alanine and aspartate metabolism | |
| MAP00330 | Arginine and proline metabolism |
| Compound table | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Substrates | Products | Intermediates | |||||||
| KEGG-id | C03406 | C00122 | C00062 | ||||||
| E.C. | |||||||||
| Compound | N-(L-Arginino)succinate | Fumarate | L-Arginine | ||||||
| Type | amino acids,amine group,carboxyl group,imine group | carboxyl group | amino acids,amine group,imine group,lipid | ||||||
| ChEBI |
18012 18012 |
16467 16467 |
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| PubChem |
16950 16950 |
21883788 444972 21883788 444972 |
28782 6322 28782 6322 |
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| 1tj7A01 |
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Unbound | Unbound | Unbound | |
| 1tj7B01 |
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Unbound | Unbound | Unbound | |
| 1auwA01 |
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Unbound | Unbound | Unbound | |
| 1auwB01 |
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Unbound | Unbound | Unbound | |
| 1auwC01 |
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Unbound | Unbound | Unbound | |
| 1auwD01 |
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Unbound | Unbound | Unbound | |
| 1dcnA01 |
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Unbound | Unbound | Unbound | |
| 1dcnB01 |
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Unbound | Unbound | Unbound | |
| 1dcnC01 |
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Unbound | Unbound | Unbound | |
| 1dcnD01 |
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Unbound | Unbound | Unbound | |
| 1hy1A01 |
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Unbound | Unbound | Unbound | |
| 1hy1B01 |
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Unbound | Unbound | Unbound | |
| 1hy1C01 |
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Unbound | Unbound | Unbound | |
| 1hy1D01 |
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Unbound | Unbound | Unbound | |
| 1k7wA01 |
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Unbound | Unbound | Unbound | |
| 1k7wB01 |
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Unbound | Unbound | Unbound | |
| 1k7wC01 |
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Unbound | Unbound | Unbound | |
| 1k7wD01 |
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Unbound | Unbound | Unbound | |
| 1tjuA01 |
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Unbound | Unbound | Unbound | |
| 1tjuB01 |
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Unbound | Unbound | Unbound | |
| 1tjuC01 |
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Unbound | Unbound | Unbound | |
| 1tjuD01 |
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Unbound | Unbound | Unbound | |
| 1tjvA01 |
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Unbound | Unbound | Unbound | |
| 1tjvB01 |
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Unbound | Unbound | Unbound | |
| 1tjvC01 |
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Unbound | Unbound | Unbound | |
| 1tjvD01 |
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Unbound | Unbound | Unbound | |
| 1tjwA01 |
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Unbound | Unbound | Unbound | |
| 1tjwB01 |
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Unbound | Unbound | Unbound | |
| 1tjwC01 |
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Unbound | Unbound | Unbound | |
| 1tjwD01 |
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Unbound | Unbound | Unbound | |
| 1k62A01 |
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Unbound | Unbound | Unbound | |
| 1k62B01 |
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Unbound | Unbound | Unbound | |
| 1aosA01 |
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Unbound | Unbound | Unbound | |
| 1aosB01 |
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Unbound | Unbound | Unbound | |
| 1tj7A02 |
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Unbound | Unbound | Unbound | |
| 1tj7B02 |
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Unbound | Unbound | Unbound | |
| 1auwA02 |
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Unbound | Unbound | Unbound | |
| 1auwB02 |
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Unbound | Unbound | Unbound | |
| 1auwC02 |
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Unbound | Unbound | Unbound | |
| 1auwD02 |
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Unbound | Unbound | Unbound | |
| 1dcnA02 |
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Unbound | Unbound | Unbound | |
| 1dcnB02 |
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Bound:AS1 | Unbound | Unbound | |
| 1dcnC02 |
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Unbound | Unbound | Unbound | |
| 1dcnD02 |
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Unbound | Unbound | Unbound | |
| 1hy1A02 |
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Unbound | Unbound | Unbound | |
| 1hy1B02 |
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Unbound | Unbound | Unbound | |
| 1hy1C02 |
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Unbound | Unbound | Unbound | |
| 1hy1D02 |
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Unbound | Unbound | Unbound | |
| 1k7wA02 |
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Bound:AS1 | Unbound | Unbound | |
| 1k7wB02 |
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Bound:AS1 | Unbound | Unbound | |
| 1k7wC02 |
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Bound:AS1 | Unbound | Unbound | |
| 1k7wD02 |
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Bound:AS1 | Unbound | Unbound | |
| 1tjuA02 |
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Unbound | Unbound | Unbound | |
| 1tjuB02 |
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Unbound | Unbound | Unbound | |
| 1tjuC02 |
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Unbound | Unbound | Unbound | |
| 1tjuD02 |
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Unbound | Unbound | Unbound | |
| 1tjvA02 |
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Unbound | Unbound | Unbound | |
| 1tjvB02 |
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Unbound | Unbound | Unbound | |
| 1tjvC02 |
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Unbound | Unbound | Unbound | |
| 1tjvD02 |
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Unbound | Unbound | Unbound | |
| 1tjwA02 |
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Bound:AS1 | Unbound | Unbound | |
| 1tjwB02 |
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Bound:AS1 | Unbound | Unbound | |
| 1tjwC02 |
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Bound:AS1 | Unbound | Unbound | |
| 1tjwD02 |
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Bound:AS1 | Unbound | Unbound | |
| 1k62A02 |
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Unbound | Unbound | Unbound | |
| 1k62B02 |
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Unbound | Unbound | Unbound | |
| 1aosA02 |
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Unbound | Unbound | Unbound | |
| 1aosB02 |
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Unbound | Unbound | Unbound | |
| 1tj7A03 |
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Unbound | Unbound | Unbound | |
| 1tj7B03 |
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Unbound | Unbound | Unbound | |
| 1auwA03 |
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Unbound | Unbound | Unbound | |
| 1auwB03 |
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Unbound | Unbound | Unbound | |
| 1auwC03 |
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Unbound | Unbound | Unbound | |
| 1auwD03 |
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Unbound | Unbound | Unbound | |
| 1dcnA03 |
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Unbound | Unbound | Unbound | |
| 1dcnB03 |
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Unbound | Unbound | Unbound | |
| 1dcnC03 |
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Unbound | Unbound | Unbound | |
| 1dcnD03 |
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Unbound | Unbound | Unbound | |
| 1hy1A03 |
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Unbound | Unbound | Unbound | |
| 1hy1B03 |
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Unbound | Unbound | Unbound | |
| 1hy1C03 |
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Unbound | Unbound | Unbound | |
| 1hy1D03 |
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Unbound | Unbound | Unbound | |
| 1k7wA03 |
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Unbound | Unbound | Unbound | |
| 1k7wB03 |
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Unbound | Unbound | Unbound | |
| 1k7wC03 |
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Unbound | Unbound | Unbound | |
| 1k7wD03 |
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Unbound | Unbound | Unbound | |
| 1tjuA03 |
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Unbound | Unbound | Unbound | |
| 1tjuB03 |
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Unbound | Unbound | Unbound | |
| 1tjuC03 |
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Unbound | Unbound | Unbound | |
| 1tjuD03 |
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Unbound | Unbound | Unbound | |
| 1tjvA03 |
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Unbound | Unbound | Unbound | |
| 1tjvB03 |
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Unbound | Unbound | Unbound | |
| 1tjvC03 |
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Unbound | Unbound | Unbound | |
| 1tjvD03 |
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Unbound | Unbound | Unbound | |
| 1tjwA03 |
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Unbound | Unbound | Unbound | |
| 1tjwB03 |
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Unbound | Unbound | Unbound | |
| 1tjwC03 |
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Unbound | Unbound | Unbound | |
| 1tjwD03 |
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Unbound | Unbound | Unbound | |
| 1k62A03 |
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Unbound | Unbound | Unbound | |
| 1k62B03 |
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Unbound | Unbound | Unbound | |
| 1aosA03 |
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Unbound | Unbound | Unbound | |
| 1aosB03 |
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Unbound | Unbound | Unbound | |
| Reference for Active-site residues | ||
|---|---|---|
| resource | references | E.C. |
| PDB;1auw & literature [14], [17], [21] & Catalytic Site Atlas | ||
| Active-site residues | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| PDB | Catalytic residues | Cofactor-binding residues | Modified residues | Main-chain involved in catalysis | Comment | |||||
| 1tj7A01 |
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| 1tj7B01 |
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| 1auwA01 |
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| 1auwB01 |
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| 1auwC01 |
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| 1auwD01 |
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| 1dcnA01 |
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| 1dcnB01 |
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| 1dcnC01 |
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| 1dcnD01 |
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| 1hy1A01 |
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| 1hy1B01 |
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| 1hy1C01 |
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| 1hy1D01 |
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| 1k7wA01 |
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| 1k7wB01 |
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| 1k7wC01 |
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| 1k7wD01 |
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| 1tjuA01 |
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| 1tjuB01 |
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| 1tjuC01 |
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| 1tjuD01 |
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| 1tjvA01 |
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| 1tjvB01 |
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| 1tjvC01 |
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| 1tjvD01 |
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| 1tjwA01 |
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| 1tjwB01 |
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| 1tjwC01 |
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| 1tjwD01 |
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| 1k62A01 |
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| 1k62B01 |
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| 1aosA01 |
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| 1aosB01 |
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| 1tj7A02 |
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HIS 156;SER 277;LYS 283;GLU 290 | ||||
| 1tj7B02 |
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HIS 156;SER 277;LYS 283;GLU 290 | ||||
| 1auwA02 |
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HIS 160;SER 281;LYS 287;GLU 294 | ||||
| 1auwB02 |
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HIS 160;SER 281;LYS 287;GLU 294 | ||||
| 1auwC02 |
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HIS 160;SER 281;LYS 287;GLU 294 | ||||
| 1auwD02 |
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HIS 160;SER 281;LYS 287;GLU 294 | ||||
| 1dcnA02 |
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; ; ;GLU 296 | mutant H162N, invisible 282-290 | |||
| 1dcnB02 |
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; ; ;GLU 296 | mutant H162N, invisible 277-291 | |||
| 1dcnC02 |
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; ; ;GLU 296 | mutant H162N, invisible 279-291 | |||
| 1dcnD02 |
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; ; ;GLU 296 | mutant H162N, invisible 282-290 | |||
| 1hy1A02 |
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HIS 160;SER 281;LYS 287;GLU 294 | ||||
| 1hy1B02 |
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HIS 160;SER 281;LYS 287;GLU 294 | ||||
| 1hy1C02 |
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HIS 160;SER 281;LYS 287;GLU 294 | ||||
| 1hy1D02 |
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HIS 160;SER 281;LYS 287;GLU 294 | ||||
| 1k7wA02 |
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HIS 162;;LYS 289;GLU 296 | mutant S283A | |||
| 1k7wB02 |
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HIS 162;;LYS 289;GLU 296 | mutant S283A | |||
| 1k7wC02 |
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HIS 162;;LYS 289;GLU 296 | mutant S283A | |||
| 1k7wD02 |
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HIS 162;;LYS 289;GLU 296 | mutant S283A | |||
| 1tjuA02 |
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HIS 162;SER 283;LYS 289;GLU 296 | mutant T161S | |||
| 1tjuB02 |
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HIS 162;SER 283;LYS 289;GLU 296 | mutant T161S | |||
| 1tjuC02 |
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HIS 162;SER 283;LYS 289;GLU 296 | mutant T161S | |||
| 1tjuD02 |
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HIS 162;SER 283;LYS 289;GLU 296 | mutant T161S | |||
| 1tjvA02 |
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HIS 162;SER 283;LYS 289;GLU 296 | mutant T161D | |||
| 1tjvB02 |
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HIS 162;SER 283;LYS 289;GLU 296 | mutant T161D | |||
| 1tjvC02 |
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HIS 162;SER 283;LYS 289;GLU 296 | mutant T161D | |||
| 1tjvD02 |
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HIS 162;SER 283;LYS 289;GLU 296 | mutant T161D | |||
| 1tjwA02 |
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HIS 162;SER 283;LYS 289;GLU 296 | mutant T161D | |||
| 1tjwB02 |
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HIS 162;SER 283;LYS 289;GLU 296 | mutant T161D | |||
| 1tjwC02 |
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HIS 162;SER 283;LYS 289;GLU 296 | mutant T161D | |||
| 1tjwD02 |
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HIS 162;SER 283;LYS 289;GLU 296 | mutant T161D | |||
| 1k62A02 |
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HIS 160;SER 281;LYS 287;GLU 294 | mutant Q286R | |||
| 1k62B02 |
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HIS 160;SER 281;LYS 287;GLU 294 | mutant Q286R | |||
| 1aosA02 |
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HIS 160;SER 281;LYS 287;GLU 294 | ||||
| 1aosB02 |
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HIS 160;SER 281;LYS 287;GLU 294 | ||||
| 1tj7A03 |
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| 1tj7B03 |
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| 1auwA03 |
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| 1auwB03 |
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| 1auwC03 |
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| 1auwD03 |
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| 1dcnA03 |
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| 1dcnB03 |
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| 1dcnC03 |
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| 1dcnD03 |
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| 1hy1A03 |
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| 1hy1B03 |
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| 1hy1C03 |
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| 1hy1D03 |
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| 1k7wA03 |
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| 1k7wB03 |
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| 1k7wC03 |
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| 1k7wD03 |
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| References for Catalytic Mechanism | ||
|---|---|---|
| References | Sections | No. of steps in catalysis |
|
[6]
|
p.730-732 | |
|
[8]
|
p.14018-14019 | |
|
[9]
|
p.9066-9067 | |
|
[10]
|
Scheme 2, p.333-334 | 3 |
|
[11]
|
p.2441-2442 | |
|
[12]
|
p.2427-2429 | |
|
[13]
|
Fig.5, p.1643-1645 | 3 |
|
[14]
|
p.2739-2741 | |
|
[15]
|
p.15575 | |
|
[16]
|
Fig.6, p.4173-4174 | 3 |
|
[18]
|
Fig.6, p.445-446 | |
| References | |
|---|---|
| [1] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 495948 |
| Journal | Anal Biochem |
| Year | 1979 |
| Volume | 95 |
| Pages | 139-55 |
| Authors | Murakami-Murofushi K, Ratner S |
| Title |
Argininosuccinase from bovine brain: isolation and comparison of catalytic, |
| Related PDB | |
| Related UniProtKB | |
| [2] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 6615795 |
| Journal | Biochemistry |
| Year | 1983 |
| Volume | 22 |
| Pages | 3729-35 |
| Authors | Garrard LJ, Mathis JM, Raushel FM |
| Title | Substrate-induced inactivation of argininosuccinate lyase by monofluorofumarate and difluorofumarate. |
| Related PDB | |
| Related UniProtKB | |
| [3] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 6722123 |
| Journal | Biochemistry |
| Year | 1984 |
| Volume | 23 |
| Pages | 1791-5 |
| Authors | Raushel FM, Garrard LJ |
| Title | A positional isotope exchange study of the argininosuccinate lyase reaction. |
| Related PDB | |
| Related UniProtKB | |
| [4] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 3522565 |
| Journal | J Biol Chem |
| Year | 1986 |
| Volume | 261 |
| Pages | 8163-6 |
| Authors | Kim SC, Raushel FM |
| Title | The determination of enzyme-substrate dissociation rates by dynamic isotope exchange enhancement experiments. |
| Related PDB | |
| Related UniProtKB | |
| [5] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8196062 |
| Journal | J Mol Biol |
| Year | 1994 |
| Volume | 239 |
| Pages | 336-8 |
| Authors | Turner MA, Achyuthan AM, Hershfield MS, McInnes RR, Howell PL |
| Title |
Expression, |
| Related PDB | |
| Related UniProtKB | |
| [6] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7634077 |
| Journal | Nat Struct Biol |
| Year | 1994 |
| Volume | 1 |
| Pages | 724-34 |
| Authors | Simpson A, Bateman O, Driessen H, Lindley P, Moss D, Mylvaganam S, Narebor E, Slingsby C |
| Title | The structure of avian eye lens delta-crystallin reveals a new fold for a superfamily of oligomeric enzymes. |
| Related PDB | |
| Related UniProtKB | |
| [7] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7613869 |
| Journal | Structure |
| Year | 1995 |
| Volume | 3 |
| Pages | 403-12 |
| Authors | Simpson A, Moss D, Slingsby C |
| Title | The avian eye lens protein delta-crystallin shows a novel packing arrangement of tetramers in a supramolecular helix. |
| Related PDB | |
| Related UniProtKB | |
| [8] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF MUTANT ASN-91. |
| Medline ID | 98039131 |
| PubMed ID | 9369472 |
| Journal | Biochemistry |
| Year | 1997 |
| Volume | 36 |
| Pages | 14012-22 |
| Authors | Abu-Abed M, Turner MA, Vallee F, Simpson A, Slingsby C, Howell PL |
| Title | Structural comparison of the enzymatically active and inactive forms of delta crystallin and the role of histidine 91. |
| Related PDB | 1auw |
| Related UniProtKB | P24058 |
| [9] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9256435 |
| Journal | Proc Natl Acad Sci U S A |
| Year | 1997 |
| Volume | 94 |
| Pages | 9063-8 |
| Authors | Turner MA, Simpson A, McInnes RR, Howell PL |
| Title | Human argininosuccinate lyase: a structural basis for intragenic complementation. |
| Related PDB | 1aos |
| Related UniProtKB | |
| [10] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9657972 |
| Journal | Biochem J |
| Year | 1998 |
| Volume | 333 |
| Pages | 327-34 |
| Authors | Wu CY, Lee HJ, Wu SH, Chen ST, Chiou SH, Chang GG |
| Title | Chemical mechanism of the endogenous argininosuccinate lyase activity of duck lens delta2-crystallin. |
| Related PDB | |
| Related UniProtKB | |
| [11] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10029537 |
| Journal | Biochemistry |
| Year | 1999 |
| Volume | 38 |
| Pages | 2435-43 |
| Authors | Chakraborty AR, Davidson A, Howell PL |
| Title | Mutational analysis of amino acid residues involved in argininosuccinate lyase activity in duck delta II crystallin. |
| Related PDB | |
| Related UniProtKB | |
| [12] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF MUTANT ASN-162. |
| Medline ID | 99155215 |
| PubMed ID | 10029536 |
| Journal | Biochemistry |
| Year | 1999 |
| Volume | 38 |
| Pages | 2425-34 |
| Authors | Vallee F, Turner MA, Lindley PL, Howell PL |
| Title | Crystal structure of an inactive duck delta II crystallin mutant with bound argininosuccinate. |
| Related PDB | 1dcn |
| Related UniProtKB | P24058 |
| [13] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11092456 |
| Journal | Cell Mol Life Sci |
| Year | 2000 |
| Volume | 57 |
| Pages | 1637-51 |
| Authors | Yu B, Howell PL |
| Title | Intragenic complementation and the structure and function of argininosuccinate lyase. |
| Related PDB | |
| Related UniProtKB | |
| [14] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11258884 |
| Journal | Biochemistry |
| Year | 2001 |
| Volume | 40 |
| Pages | 2732-42 |
| Authors | Sampaleanu LM, Vallee F, Slingsby C, Howell PL |
| Title | Structural studies of duck delta 1 and delta 2 crystallin suggest conformational changes occur during catalysis. |
| Related PDB | 1hy1 1hy0 1i0a |
| Related UniProtKB | |
| [15] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11747432 |
| Journal | Biochemistry |
| Year | 2001 |
| Volume | 40 |
| Pages | 15570-80 |
| Authors | Sampaleanu LM, Vallee F, Thompson GD, Howell PL |
| Title | Three-dimensional structure of the argininosuccinate lyase frequently complementing allele Q286R. |
| Related PDB | 1k62 |
| Related UniProtKB | |
| [16] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11698398 |
| Journal | J Biol Chem |
| Year | 2002 |
| Volume | 277 |
| Pages | 4166-75 |
| Authors | Sampaleanu LM, Yu B, Howell PL |
| Title | Mutational analysis of duck delta 2 crystallin and the structure of an inactive mutant with bound substrate provide insight into the enzymatic mechanism of argininosuccinate lyase. |
| Related PDB | 1k7w |
| Related UniProtKB | |
| [17] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 15502303 |
| Journal | Acta Crystallogr D Biol Crystallogr |
| Year | 2004 |
| Volume | 60 |
| Pages | 1964-70 |
| Authors | Bhaumik P, Koski MK, Bergmann U, Wierenga RK |
| Title | Structure determination and refinement at 2.44 A resolution of argininosuccinate lyase from Escherichia coli. |
| Related PDB | 1tj7 |
| Related UniProtKB | |
| [18] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 15320872 |
| Journal | Biochem J |
| Year | 2004 |
| Volume | 384 |
| Pages | 437-47 |
| Authors | Sampaleanu LM, Codding PW, Lobsanov YD, Tsai M, Smith GD, Horvatin C, Howell PL |
| Title | Structural studies of duck delta2 crystallin mutants provide insight into the role of Thr161 and the 280s loop in catalysis. |
| Related PDB | 1tju 1tjv 1tjw |
| Related UniProtKB | |
| [19] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 15362851 |
| Journal | Biochemistry |
| Year | 2004 |
| Volume | 43 |
| Pages | 11672-82 |
| Authors | Tsai M, Sampaleanu LM, Greene C, Creagh L, Haynes C, Howell PL |
| Title | A duck delta1 crystallin double loop mutant provides insight into residues important for argininosuccinate lyase activity. |
| Related PDB | 1u15 1u16 |
| Related UniProtKB | |
| Comments |
|---|
|
There are two delta crystallin isoforms in duck lenses, According to the literature [9] & [10], (1) Glu294 (of PDB;1aos) makes His160 a general acid, (2) His160 abstracts a proton from the C-beta of substrate (or deprotonation site of leaving group), (3) Redistribution of the negative charge in the leaving group (fumarate group) gives an aci-carboxylate intermediate. (4) Ser281 acts as a general acid, |
| Created | Updated |
|---|---|
| 2004-06-28 | 2009-02-26 |