DB code: S00416
| CATH domain | 3.40.718.10 : Isopropylmalate Dehydrogenase | Catalytic domain |
|---|---|---|
| E.C. | 1.1.1.42 | |
| CSA | ||
| M-CSA | ||
| MACiE | M0007 | |
| CATH domain | Related DB codes (homologues) |
|---|---|
| 3.40.718.10 : Isopropylmalate Dehydrogenase | S00417 |
| Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | RefSeq | Pfam |
|---|---|---|---|---|
| Q9YE81 |
|
Isocitrate dehydrogenase {NADP}
EC 1.1.1.42 |
NP_147421.2
(Protein)
NC_000854.2 (DNA/RNA sequence) |
PF00180
(Iso_dh)
[Graphical View] |
| P08200 |
Isocitrate dehydrogenase {NADP}
|
IDH
EC 1.1.1.42 Oxalosuccinate decarboxylase NADP(+)-specific ICDH IDP |
NP_415654.1
(Protein)
NC_000913.2 (DNA/RNA sequence) YP_489404.1 (Protein) NC_007779.1 (DNA/RNA sequence) |
PF00180
(Iso_dh)
[Graphical View] |
| P39126 |
Isocitrate dehydrogenase {NADP}
|
IDH
EC 1.1.1.42 Oxalosuccinate decarboxylase NADP(+)-specific ICDH IDP |
NP_390791.1
(Protein)
NC_000964.3 (DNA/RNA sequence) |
PF00180
(Iso_dh)
[Graphical View] |
| P33198 |
Isocitrate dehydrogenase {NADP}, mitochondrial
|
IDH
EC 1.1.1.42 Oxalosuccinate decarboxylase NADP(+)-specific ICDH IDP ICD-M |
PF00180
(Iso_dh)
[Graphical View] |
|
| O75874 |
Isocitrate dehydrogenase {NADP} cytoplasmic
|
IDH
EC 1.1.1.42 Cytosolic NADP-isocitrate dehydrogenase Oxalosuccinate decarboxylase NADP(+)-specific ICDH IDP |
NP_005887.2
(Protein)
NM_005896.2 (DNA/RNA sequence) |
PF00180
(Iso_dh)
[Graphical View] |
| KEGG enzyme name |
|---|
|
isocitrate dehydrogenase (NADP+)
oxalosuccinate decarboxylase oxalsuccinic decarboxylase isocitrate (NADP) dehydrogenase isocitrate (nicotinamide adenine dinucleotide phosphate)dehydrogenase NADP-specific isocitrate dehydrogenase NADP-linked isocitrate dehydrogenase NADP-dependent isocitrate dehydrogenase NADP isocitric dehydrogenase isocitrate dehydrogenase (NADP-dependent) NADP-dependent isocitric dehydrogenase triphosphopyridine nucleotide-linked isocitratedehydrogenase-oxalosuccinate carboxylase NADP+-linked isocitrate dehydrogenase IDH (ambiguous) dual-cofactor-specific isocitrate dehydrogenase NADP+-ICDH NADP+-IDH IDP IDP1 IDP2 IDP3 |
| UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
|---|---|---|---|---|---|
| Q9YE81 | Q9YE81_AERPE | ||||
| P08200 | IDH_ECOLI | Isocitrate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH. Oxalosuccinate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH. | Homodimer. | Binds 1 magnesium or manganese ion per subunit. | |
| P39126 | IDH_BACSU | Isocitrate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH. Oxalosuccinate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH. | Homodimer. | Binds 1 magnesium or manganese ion per subunit (By similarity). | |
| P33198 | IDHP_PIG | Isocitrate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH. Oxalosuccinate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH. | Homodimer. | Mitochondrion. | Binds 1 magnesium or manganese ion per subunit. |
| O75874 | IDHC_HUMAN | Isocitrate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH. Oxalosuccinate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH. | Homodimer. | Cytoplasm. Peroxisome. | Binds 1 magnesium or manganese ion per subunit. |
| KEGG Pathways | Map code | Pathways | E.C. |
|---|---|---|
| MAP00020 | Citrate cycle (TCA cycle) | |
| MAP00480 | Glutathione metabolism | |
| MAP00720 | Reductive carboxylate cycle (CO2 fixation) |
| Compound table | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cofactors | Substrates | Products | Intermediates | ||||||||||
| KEGG-id | C00572 | C00006 | C00311 | C00005 | C00011 | C00026 | C00080 | C05379 | |||||
| E.C. | |||||||||||||
| Compound | Divalent cation (Mg or Mn) | NADP+ | Isocitrate | NADPH | CO2 | 2-Oxoglutarate | H+ | Oxalosuccinate | |||||
| Type | divalent metal (Ca2+, Mg2+) | amide group,amine group,nucleotide | carbohydrate,carboxyl group | amide group,amine group,nucleotide | others | carbohydrate,carboxyl group | others | ||||||
| ChEBI |
18009 18009 |
30887 30887 |
16474 16474 |
16526 16526 |
30915 30915 |
15378 15378 |
|||||||
| PubChem |
5886 5886 |
1198 1198 |
5884 5884 |
280 280 |
51 51 |
1038 1038 |
|||||||
| 1tyoA |
|
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|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1tyoB |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1v94A |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1v94B |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1xgvA |
|
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|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1xgvB |
|
|
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|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1xkdA |
|
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|
|
Analogue:_CA | Bound:NAP | Bound:ICT | Unbound | Unbound | Unbound | Unbound | |
| 1xkdB |
|
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|
Analogue:_CA | Bound:NAP | Bound:ICT | Unbound | Unbound | Unbound | Unbound | |
| 1ai2A |
|
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|
Analogue:ICA | Bound:NAP | Bound:ICA | Unbound | Unbound | Unbound | Unbound | |
| 1ai3A |
|
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|
|
Bound:ITM | Analogue:NDO | Bound:ITM | Unbound | Unbound | Unbound | Unbound | |
| 1bl5A |
|
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|
|
|
Bound:_MG | Analogue:NAP | Unbound | Unbound | Unbound | Bound:AKG | Unbound | |
| 1cw1A |
|
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|
|
Analogue:_MN | Unbound | Bound:ICT | Unbound | Unbound | Unbound | Unbound | |
| 1cw4A |
|
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|
Analogue:_MN | Unbound | Unbound | Unbound | Unbound | Bound:AKG | Unbound | |
| 1cw7A |
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|
Bound:_MG | Unbound | Bound:ICT | Unbound | Unbound | Unbound | Unbound | |
| 1groA |
|
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Bound:_MG | Unbound | Bound:ICT | Unbound | Unbound | Unbound | Unbound | |
| 1grpA |
|
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|
Bound:_MG | Unbound | Bound:ICT | Unbound | Unbound | Unbound | Unbound | |
| 1hj6A |
|
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|
|
|
Bound:_MG | Bound:NAP | Analogue:IPM | Unbound | Unbound | Unbound | Unbound | |
| 1idcA |
|
|
|
|
|
Bound:_MG | Unbound | Unbound | Unbound | Unbound | Unbound | Intermediate-bound:OXS | |
| 1iddA |
|
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|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ideA |
|
|
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|
Bound:_MG | Bound:NAP | Bound:ICT | Unbound | Unbound | Unbound | Unbound | |
| 1idfA |
|
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|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ikaA |
|
|
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|
Analogue:_CA | Unbound | Unbound | Unbound | Unbound | Bound:AKG | Unbound | |
| 1ikbA |
|
|
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|
Analogue:_CA | Bound:NAP | Bound:ICT | Unbound | Unbound | Unbound | Unbound | |
| 1isoA |
|
|
|
|
|
Unbound | Analogue:AMP | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1p8fA |
|
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|
|
Bound:_MG | Unbound | Bound:ICT | Unbound | Unbound | Unbound | Unbound | |
| 1pb1A |
|
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|
|
Unbound | Unbound | Bound:ICT | Unbound | Unbound | Unbound | Unbound | |
| 1pb3A |
|
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|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1sjsA |
|
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|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 3icdA |
|
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 4icdA |
|
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|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 5icdA |
|
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Bound:_MG | Unbound | Bound:ICT | Unbound | Unbound | Unbound | Unbound | |
| 6icdA |
|
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|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 7icdA |
|
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|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 8icdA |
|
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|
|
|
Bound:_MG | Unbound | Bound:ICT | Unbound | Unbound | Unbound | Unbound | |
| 9icdA |
|
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|
Unbound | Analogue:NAP | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1hqsA |
|
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Unbound | Unbound | Analogue:CIT | Unbound | Unbound | Unbound | Unbound | |
| 1hqsB |
|
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Unbound | Unbound | Analogue:CIT | Unbound | Unbound | Unbound | Unbound | |
| 1lwdA |
|
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Analogue:_MN | Unbound | Bound:ICT | Unbound | Unbound | Unbound | Unbound | |
| 1lwdB |
|
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|
Analogue:_MN | Unbound | Bound:ICT | Unbound | Unbound | Unbound | Unbound | |
| 1t09A |
|
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Unbound | Bound:NAP | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1t09B |
|
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Unbound | Bound:NAP | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1t0lA |
|
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Analogue:_CA | Bound:NAP | Bound:ICT | Unbound | Unbound | Unbound | Unbound | |
| 1t0lB |
|
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Analogue:_CA | Bound:NAP | Bound:ICT | Unbound | Unbound | Unbound | Unbound | |
| 1t0lC |
|
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Analogue:_CA | Bound:NAP | Bound:ICT | Unbound | Unbound | Unbound | Unbound | |
| 1t0lD |
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Analogue:_CA | Bound:NAP | Bound:ICT | Unbound | Unbound | Unbound | Unbound | |
| Reference for Active-site residues | ||
|---|---|---|
| resource | references | E.C. |
| literature [11], [15] | ||
| Active-site residues | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| PDB | Catalytic residues | Cofactor-binding residues | Modified residues | Main-chain involved in catalysis | Comment | |||||
| 1tyoA |
|
|
|
|
|
TYR 166;LYS 233;ASP 287 | ASP 311;ASP 315(Magnesium binding) | SER 120(Phosphorylation) | ||
| 1tyoB |
|
|
|
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|
TYR 166;LYS 233;ASP 287 | ASP 311;ASP 315(Magnesium binding) | SER 120(Phosphorylation) | ||
| 1v94A |
|
|
|
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|
TYR 166;LYS 233;ASP 287 | ASP 311;ASP 315(Magnesium binding) | SER 120(Phosphorylation) | ||
| 1v94B |
|
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TYR 166;LYS 233;ASP 287 | ASP 311;ASP 315(Magnesium binding) | SER 120(Phosphorylation) | ||
| 1xgvA |
|
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|
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TYR 166;LYS 233;ASP 287 | ASP 311;ASP 315(Magnesium binding) | SER 120(Phosphorylation) | ||
| 1xgvB |
|
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TYR 166;LYS 233;ASP 287 | ASP 311;ASP 315(Magnesium binding) | SER 120(Phosphorylation) | ||
| 1xkdA |
|
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|
TYR 166;LYS 233;ASP 287 | ASP 311;ASP 315(Magnesium binding) | SER 120(Phosphorylation) | ||
| 1xkdB |
|
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TYR 166;LYS 233;ASP 287 | ASP 311;ASP 315(Magnesium binding) | SER 120(Phosphorylation) | ||
| 1ai2A |
|
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|
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TYR 160;LYS 230;ASP 283 | ASP 307;ASP 311(Magnesium binding) | SER 113(Phosphorylation) | ||
| 1ai3A |
|
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TYR 160;LYS 230;ASP 283 | ASP 307;ASP 311(Magnesium binding) | SER 113(Phosphorylation) | ||
| 1bl5A |
|
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TYR 160;LYS 230;ASP 283 | ASP 307;ASP 311(Magnesium binding) | SER 113(Phosphorylation) | ||
| 1cw1A |
|
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|
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|
TYR 160;;ASP 283 | ASP 307;ASP 311(Magnesium binding) | SER 113(Phosphorylation) | mutant K230M | |
| 1cw4A |
|
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TYR 160;;ASP 283 | ASP 307;ASP 311(Manganese binding) | SER 113(Phosphorylation) | mutant K230M | |
| 1cw7A |
|
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TYR 160;LYS 230;ASP 283 | ASP 307;ASP 311(Magnesium binding) | SER 113(Phosphorylation) | ||
| 1groA |
|
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TYR 160;LYS 230;ASP 283 | ASP 307;ASP 311(Magnesium binding) | mutant S113E, N115L | ||
| 1grpA |
|
|
|
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|
TYR 160;LYS 230;ASP 283 | ASP 307;ASP 311(Magnesium binding) | SER 113(Phosphorylation) | mutant N115L | |
| 1hj6A |
|
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|
TYR 160;LYS 230;ASP 283 | ASP 307;ASP 311(Magnesium binding) | mutant S113E | ||
| 1idcA |
|
|
|
|
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TYR 160;;ASP 283 | ASP 307;ASP 311(Magnesium binding) | SER 113(Phosphorylation) | mutant K230M | |
| 1iddA |
|
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|
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|
;LYS 230;ASP 283 | ASP 307;ASP 311(Magnesium binding) | SER 113(Phosphorylation) | mutant Y160F | |
| 1ideA |
|
|
|
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;LYS 230;ASP 283 | ASP 307;ASP 311(Magnesium binding) | SER 113(Phosphorylation) | mutant Y160F | |
| 1idfA |
|
|
|
|
|
TYR 160;;ASP 283 | ASP 307;ASP 311(Magnesium binding) | SER 113(Phosphorylation) | mutant K230M | |
| 1ikaA |
|
|
|
|
|
TYR 160;LYS 230;ASP 283 | ASP 307;ASP 311(Magnesium binding) | SER 113(Phosphorylation) | ||
| 1ikbA |
|
|
|
|
|
TYR 160;LYS 230;ASP 283 | ASP 307;ASP 311(Magnesium binding) | SER 113(Phosphorylation) | ||
| 1isoA |
|
|
|
|
|
TYR 160;LYS 230;ASP 283 | ASP 307;ASP 311(Magnesium binding) | SER 113(Phosphorylation) | mutant C201M, C332Y, K344D, Y345I, V351A, Y391K, R395S | |
| 1p8fA |
|
|
|
|
|
TYR 160;LYS 230;ASP 283 | ASP 307;ASP 311(Magnesium binding) | SER 113(Phosphorylation) | ||
| 1pb1A |
|
|
|
|
|
TYR 160;LYS 230;ASP 283 | ASP 307;ASP 311(Magnesium binding) | SER 113(Phosphorylation) | ||
| 1pb3A |
|
|
|
|
|
TYR 160;LYS 230;ASP 283 | ASP 307;ASP 311(Magnesium binding) | SER 113(Phosphorylation) | ||
| 1sjsA |
|
|
|
|
|
TYR 160;LYS 230;ASP 283 | ASP 307;ASP 311(Magnesium binding) | SER 113(Phosphorylation) | ||
| 3icdA |
|
|
|
|
|
TYR 160;LYS 230;ASP 283 | ASP 307;ASP 311(Magnesium binding) | SER 113(Phosphorylation) | ||
| 4icdA |
|
|
|
|
|
TYR 160;LYS 230;ASP 283 | ASP 307;ASP 311(Magnesium binding) | SER 113(Phosphorylation) | ||
| 5icdA |
|
|
|
|
|
TYR 160;LYS 230;ASP 283 | ASP 307;ASP 311(Magnesium binding) | SER 113(Phosphorylation) | ||
| 6icdA |
|
|
|
|
|
TYR 160;LYS 230;ASP 283 | ASP 307;ASP 311(Magnesium binding) | mutant S113E | ||
| 7icdA |
|
|
|
|
|
TYR 160;LYS 230;ASP 283 | ASP 307;ASP 311(Magnesium binding) | mutant S113E | ||
| 8icdA |
|
|
|
|
|
TYR 160;LYS 230;ASP 283 | ASP 307;ASP 311(Magnesium binding) | mutant S113E | ||
| 9icdA |
|
|
|
|
|
TYR 160;LYS 230;ASP 283 | ASP 307;ASP 311(Magnesium binding) | SER 113(Phosphorylation) | ||
| 1hqsA |
|
|
|
|
|
TYR 151;LYS 221;ASP 287 | ASP 311;ASP 315(Magnesium binding) | SER 104(Phosphorylation);CME 118 | ||
| 1hqsB |
|
|
|
|
|
TYR 151;LYS 221;ASP 287 | ASP 311;ASP 315(Magnesium binding) | SER 104(Phosphorylation);CME 118 | ||
| 1lwdA |
|
|
|
|
|
TYR 140;LYS 212;ASP 252 | ASP 275;ASP 279(Magnesium binding) | SER 95(Phosphorylation) | ||
| 1lwdB |
|
|
|
|
|
TYR 140;LYS 212;ASP 252 | ASP 275;ASP 279(Magnesium binding) | SER 95(Phosphorylation) | ||
| 1t09A |
|
|
|
|
|
TYR 139;LYS 212;ASP 252 | ASP 275;ASP 279(Magnesium binding) | SER 94(Phosphorylation) | ||
| 1t09B |
|
|
|
|
|
TYR 139;LYS 212;ASP 252 | ASP 275;ASP 279(Magnesium binding) | SER 94(Phosphorylation) | ||
| 1t0lA |
|
|
|
|
|
TYR 139;LYS 212;ASP 252 | ASP 275;ASP 279(Magnesium binding) | SER 94(Phosphorylation) | ||
| 1t0lB |
|
|
|
|
|
TYR 139;LYS 212;ASP 252 | ASP 275;ASP 279(Magnesium binding) | SER 94(Phosphorylation) | ||
| 1t0lC |
|
|
|
|
|
TYR 139;LYS 212;ASP 252 | ASP 275;ASP 279(Magnesium binding) | SER 94(Phosphorylation) | ||
| 1t0lD |
|
|
|
|
|
TYR 139;LYS 212;ASP 252 | ASP 275;ASP 279(Magnesium binding) | SER 94(Phosphorylation) | ||
| References for Catalytic Mechanism | ||
|---|---|---|
| References | Sections | No. of steps in catalysis |
|
[11]
|
Fig.7, p.8675-8676 | |
|
[13]
|
p.9314, p.9315-9316 | |
|
[14]
|
Fig.1 | |
|
[15]
|
p.382-383 | |
|
[19]
|
Fig.25 | |
|
[20]
|
p.292 | |
|
[23]
|
||
|
[24]
|
Fig.1 | |
|
[27]
|
||
|
[31]
|
Fig.7, p.43461-43462 | |
|
[32]
|
||
|
[33]
|
p.33954, Fig.6 | |
|
[35]
|
p.567-568 | |
| References | |
|---|---|
| [1] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 6254771 |
| Journal | Eur J Biochem |
| Year | 1980 |
| Volume | 110 |
| Pages | 465-73 |
| Authors | Kuchel PW, Reynolds CH, Dalziel K |
| Title | Determination of the stability constants of Mn2+ and Mg2+ complexes of the components of the NADP-linked isocitrate dehydrogenase reaction by electron spin resonance. |
| Related PDB | |
| Related UniProtKB | |
| [2] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 6722120 |
| Journal | Biochemistry |
| Year | 1984 |
| Volume | 23 |
| Pages | 1675-83 |
| Authors | Mas MT, Colman RF |
| Title | Phosphorus-31 nuclear magnetic resonance studies of the binding of nucleotides to NADP+-specific isocitrate dehydrogenase. |
| Related PDB | |
| Related UniProtKB | |
| [3] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 4074702 |
| Journal | Biochemistry |
| Year | 1985 |
| Volume | 24 |
| Pages | 5378-87 |
| Authors | Ehrlich RS, Colman RF |
| Title | 1H nuclear magnetic resonance studies of the conformation and environment of nucleotides bound to pig heart NADP+-dependent isocitrate dehydrogenase. |
| Related PDB | |
| Related UniProtKB | |
| [4] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 3651391 |
| Journal | Biochemistry |
| Year | 1987 |
| Volume | 26 |
| Pages | 3461-6 |
| Authors | Ehrlich RS, Colman RF |
| Title | Ionization of isocitrate bound to pig heart NADP+-dependent isocitrate dehydrogenase: 13C NMR study of substrate binding. |
| Related PDB | |
| Related UniProtKB | |
| [5] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2753210 |
| Journal | Biochem Soc Trans |
| Year | 1989 |
| Volume | 17 |
| Pages | 307-11 |
| Authors | Colman RF |
| Title | Affinity labels and spectroscopic probes of porcine heart NADP-dependent isocitrate dehydrogenase. |
| Related PDB | |
| Related UniProtKB | |
| [6] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2541772 |
| Journal | Biochemistry |
| Year | 1989 |
| Volume | 28 |
| Pages | 2058-65 |
| Authors | Ehrlich RS, Colman RF |
| Title | Multinuclear NMR studies of the divalent metal binding site of NADP-dependent isocitrate dehydrogenase from pig heart. |
| Related PDB | |
| Related UniProtKB | |
| [7] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) |
| Medline ID | 90046847 |
| PubMed ID | 2682654 |
| Journal | Proc Natl Acad Sci U S A |
| Year | 1989 |
| Volume | 86 |
| Pages | 8635-9 |
| Authors | Hurley JH, Thorsness PE, Ramalingam V, Helmers NH, Koshland DE Jr, Stroud RM |
| Title |
Structure of a bacterial enzyme regulated by phosphorylation, |
| Related PDB | 3icd |
| Related UniProtKB | P08200 |
| [8] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2378874 |
| Journal | Biochemistry |
| Year | 1990 |
| Volume | 29 |
| Pages | 5179-87 |
| Authors | Ehrlich RS, Colman RF |
| Title |
Conformations of the coenzymes and the allosteric activator, |
| Related PDB | |
| Related UniProtKB | |
| [9] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 2406256 |
| Journal | J Biol Chem |
| Year | 1990 |
| Volume | 265 |
| Pages | 3599-602 |
| Authors | Hurley JH, Dean AM, Thorsness PE, Koshland DE Jr, Stroud RM |
| Title | Regulation of isocitrate dehydrogenase by phosphorylation involves no long-range conformational change in the free enzyme. |
| Related PDB | 4icd |
| Related UniProtKB | |
| [10] | |
| Resource | |
| Comments | INFLUENCE OF PHOSPHORYLATION |
| Medline ID | 90371294 |
| PubMed ID | 2204109 |
| Journal | Science |
| Year | 1990 |
| Volume | 249 |
| Pages | 1012-6 |
| Authors | Hurley JH, Dean AM, Sohl JL, Koshland DE Jr, Stroud RM |
| Title | Regulation of an enzyme by phosphorylation at the active site. |
| Related PDB | 5icd 6icd 7icd 8icd |
| Related UniProtKB | P08200 |
| [11] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 1888729 |
| Journal | Biochemistry |
| Year | 1991 |
| Volume | 30 |
| Pages | 8671-8 |
| Authors | Hurley JH, Dean AM, Koshland DE Jr, Stroud RM |
| Title | Catalytic mechanism of NADP(+)-dependent isocitrate dehydrogenase: implications from the structures of magnesium-isocitrate and NADP+ complexes. |
| Related PDB | 9icd |
| Related UniProtKB | |
| [12] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1463739 |
| Journal | Biochemistry |
| Year | 1992 |
| Volume | 31 |
| Pages | 12524-31 |
| Authors | Ehrlich RS, Colman RF |
| Title |
Selectivity in the binding of NAD(P)+ analogues to NAD- and NADP-dependent pig heart isocitrate dehydrogenases. |
| Related PDB | |
| Related UniProtKB | |
| [13] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8369300 |
| Journal | Biochemistry |
| Year | 1993 |
| Volume | 32 |
| Pages | 9310-6 |
| Authors | Stoddard BL, Dean A, Koshland DE Jr |
| Title |
Structure of isocitrate dehydrogenase with isocitrate, |
| Related PDB | |
| Related UniProtKB | |
| [14] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 8369301 |
| Journal | Biochemistry |
| Year | 1993 |
| Volume | 32 |
| Pages | 9317-22 |
| Authors | Stoddard BL, Koshland DE Jr |
| Title | Structure of isocitrate dehydrogenase with alpha-ketoglutarate at 2.7-A resolution: conformational changes induced by decarboxylation of isocitrate. |
| Related PDB | 1ika |
| Related UniProtKB | |
| [15] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 7819221 |
| Journal | Biochemistry |
| Year | 1995 |
| Volume | 34 |
| Pages | 378-84 |
| Authors | Lee ME, Dyer DH, Klein OD, Bolduc JM, Stoddard BL, Koshland DE Jr |
| Title | Mutational analysis of the catalytic residues lysine 230 and tyrosine 160 in the NADP(+)-dependent isocitrate dehydrogenase from Escherichia coli. |
| Related PDB | 1idd |
| Related UniProtKB | |
| [16] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7819280 |
| Journal | Biochim Biophys Acta |
| Year | 1995 |
| Volume | 1246 |
| Pages | 135-41 |
| Authors | Ehrlich RS, Colman RF |
| Title | Cadmium-113 and magnesium-25 NMR study of the divalent metal binding sites of isocitrate dehydrogenases from pig heart. |
| Related PDB | |
| Related UniProtKB | |
| [17] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 7761851 |
| Journal | Science |
| Year | 1995 |
| Volume | 268 |
| Pages | 1312-8 |
| Authors | Bolduc JM, Dyer DH, Scott WG, Singer P, Sweet RM, Koshland DE Jr, Stoddard BL |
| Title | Mutagenesis and Laue structures of enzyme intermediates: isocitrate dehydrogenase. |
| Related PDB | 1idc 1ide 1idf |
| Related UniProtKB | |
| [18] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 8639526 |
| Journal | Biochemistry |
| Year | 1996 |
| Volume | 35 |
| Pages | 5670-8 |
| Authors | Hurley JH, Chen R, Dean AM |
| Title | Determinants of cofactor specificity in isocitrate dehydrogenase: structure of an engineered NADP+ --> NAD+ specificity-reversal mutant. |
| Related PDB | 1iso |
| Related UniProtKB | |
| [19] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8888067 |
| Journal | Pharmacol Ther |
| Year | 1996 |
| Volume | 70 |
| Pages | 215-56 |
| Authors | Stoddard BL |
| Title |
Intermediate trapping and laue X-ray diffraction: potential for enzyme mechanism, |
| Related PDB | |
| Related UniProtKB | |
| [20] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 8745407 |
| Journal | Protein Sci |
| Year | 1996 |
| Volume | 5 |
| Pages | 287-95 |
| Authors | Chen R, Grobler JA, Hurley JH, Dean AM |
| Title | Second-site suppression of regulatory phosphorylation in Escherichia coli isocitrate dehydrogenase. |
| Related PDB | 1gro 1grp |
| Related UniProtKB | |
| [21] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9220992 |
| Journal | Biochemistry |
| Year | 1997 |
| Volume | 36 |
| Pages | 9035-44 |
| Authors | Cohen BE, Stoddard BL, Koshland DE Jr |
| Title |
Caged NADP and NAD. |
| Related PDB | |
| Related UniProtKB | |
| [22] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 9374867 |
| Journal | Biochemistry |
| Year | 1997 |
| Volume | 36 |
| Pages | 13890-6 |
| Authors | Finer-Moore J, Tsutakawa SE, Cherbavaz DR, LaPorte DC, Koshland DE Jr, Stroud RM |
| Title | Access to phosphorylation in isocitrate dehydrogenase may occur by domain shifting. |
| Related PDB | 1sjs |
| Related UniProtKB | |
| [23] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 9211842 |
| Journal | Science |
| Year | 1997 |
| Volume | 277 |
| Pages | 202-6 |
| Authors | Mesecar AD, Stoddard BL, Koshland DE Jr |
| Title | Orbital steering in the catalytic power of enzymes: small structural changes with large catalytic consequences. |
| Related PDB | 1ai2 1ai3 1ikb |
| Related UniProtKB | |
| [24] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 9783749 |
| Journal | Nat Struct Biol |
| Year | 1998 |
| Volume | 5 |
| Pages | 891-7 |
| Authors | Stoddard BL, Cohen BE, Brubaker M, Mesecar AD, Koshland DE Jr |
| Title | Millisecond Laue structures of an enzyme-product complex using photocaged substrate analogs. |
| Related PDB | 1bl5 |
| Related UniProtKB | |
| [25] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10779683 |
| Journal | Biochim Biophys Acta |
| Year | 2000 |
| Volume | 1474 |
| Pages | 321-30 |
| Authors | Rao AV, Ramasarma T |
| Title |
NADH-dependent decavanadate reductase, |
| Related PDB | |
| Related UniProtKB | |
| [26] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10902579 |
| Journal | IUBMB Life |
| Year | 2000 |
| Volume | 49 |
| Pages | 457-66 |
| Authors | Mesecar AD, Koshland DE Jr |
| Title | Sites of binding and orientation in a four-location model for protein stereospecificity. |
| Related PDB | 1pb3 |
| Related UniProtKB | |
| [27] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF MUTANT MET-230 |
| Medline ID | 20090939 |
| PubMed ID | 10623532 |
| Journal | J Mol Biol |
| Year | 2000 |
| Volume | 295 |
| Pages | 377-85 |
| Authors | Cherbavaz DB, Lee ME, Stroud RM, Koshland DE Jr |
| Title | Active site water molecules revealed in the 2.1 A resolution structure of a site-directed mutant of isocitrate dehydrogenase. |
| Related PDB | 1cw1 1cw4 1cw7 |
| Related UniProtKB | P08200 |
| [28] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10688187 |
| Journal | Nature |
| Year | 2000 |
| Volume | 403 |
| Pages | 614-5 |
| Authors | Mesecar AD, Koshland DE Jr |
| Title | A new model for protein stereospecificity. |
| Related PDB | 1p8f 1pb1 |
| Related UniProtKB | |
| [29] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 11284679 |
| Journal | Biochemistry |
| Year | 2001 |
| Volume | 40 |
| Pages | 4234-41 |
| Authors | Doyle SA, Beernink PT, Koshland DE Jr |
| Title |
Structural basis for a change in substrate specificity: crystal structure of S113E isocitrate dehydrogenase in a complex with isopropylmalate, |
| Related PDB | 1hj6 |
| Related UniProtKB | |
| [30] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (1.55 ANGSTROMS). |
| Medline ID | |
| PubMed ID | 11290745 |
| Journal | J Biol Chem |
| Year | 2001 |
| Volume | 276 |
| Pages | 26154-63 |
| Authors | Singh SK, Matsuno K, LaPorte DC, Banaszak LJ |
| Title |
Crystal structure of Bacillus subtilis isocitrate dehydrogenase at 1.55 A. |
| Related PDB | 1hqs |
| Related UniProtKB | P39126 |
| [31] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12207025 |
| Journal | J Biol Chem |
| Year | 2002 |
| Volume | 277 |
| Pages | 43454-62 |
| Authors | Ceccarelli C, Grodsky NB, Ariyaratne N, Colman RF, Bahnson BJ |
| Title |
Crystal structure of porcine mitochondrial NADP+-dependent isocitrate dehydrogenase complexed with Mn2+ and isocitrate. |
| Related PDB | 1lwd |
| Related UniProtKB | |
| [32] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 14512428 |
| Journal | J Biol Chem |
| Year | 2003 |
| Volume | 278 |
| Pages | 49323-31 |
| Authors | Kim TK, Lee P, Colman RF |
| Title | Critical role of Lys212 and Tyr140 in porcine NADP-dependent isocitrate dehydrogenase. |
| Related PDB | |
| Related UniProtKB | |
| [33] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 15173171 |
| Journal | J Biol Chem |
| Year | 2004 |
| Volume | 279 |
| Pages | 33946-57 |
| Authors | Xu X, Zhao J, Xu Z, Peng B, Huang Q, Arnold E, Ding J |
| Title | Structures of human cytosolic NADP-dependent isocitrate dehydrogenase reveal a novel self-regulatory mechanism of activity. |
| Related PDB | 1t09 1t0l |
| Related UniProtKB | |
| [34] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 15146507 |
| Journal | Proteins |
| Year | 2004 |
| Volume | 55 |
| Pages | 1087-9 |
| Authors | Jeong JJ, Sonoda T, Fushinobu S, Shoun H, Wakagi T |
| Title | Crystal structure of isocitrate dehydrogenase from Aeropyrum pernix. |
| Related PDB | 1v94 |
| Related UniProtKB | |
| [35] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 15581899 |
| Journal | J Mol Biol |
| Year | 2005 |
| Volume | 345 |
| Pages | 559-77 |
| Authors | Karlstrom M, Stokke R, Steen IH, Birkeland NK, Ladenstein R |
| Title | Isocitrate dehydrogenase from the hyperthermophile Aeropyrum pernix: X-ray structure analysis of a ternary enzyme-substrate complex and thermal stability. |
| Related PDB | 1tyo 1xgv 1xkd |
| Related UniProtKB | |
| Comments |
|---|
|
This enzyme can be inactivated by phosphorylation of Ser113 (of 5icd) (see literature [10]).
According to the literature [11], (A) Hydride transfer from substrate to NADP(+): (B) Eliminative double-bond formation (or decarboxylation): (C) Isomerization (shift of double-bond position): |
| Created | Updated |
|---|---|
| 2004-07-22 | 2009-02-26 |