DB code: S00279

CATH domain 3.40.30.10 : Glutaredoxin Catalytic domain
E.C. 1.11.1.9
CSA 1gp1
M-CSA 1gp1
MACiE

CATH domain Related DB codes (homologues)
3.40.30.10 : Glutaredoxin S00916 M00184 D00866 D00869 D00870 D00278

Uniprot Enzyme Name
UniprotKB Protein name Synonyms RefSeq Pfam
P00435 Glutathione peroxidase 1
EC 1.11.1.9
GSHPx-1
GPx-1
Cellular glutathione peroxidase
NP_776501.1 (Protein)
NM_174076.3 (DNA/RNA sequence)
PF00255 (GSHPx)
[Graphical View]

KEGG enzyme name
glutathione peroxidase
GSH peroxidase
selenium-glutathione peroxidase
reduced glutathione peroxidase

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P00435 GPX1_BOVIN 2 glutathione + H(2)O(2) = glutathione disulfide + 2 H(2)O. Homotetramer. Cytoplasm.

KEGG Pathways
Map code Pathways E.C.
MAP00480 Glutathione metabolism
MAP00590 Arachidonic acid metabolism

Compound table
Cofactors Substrates Products Intermediates
KEGG-id C01529 C00051 C00027 C00127 C00001
E.C.
Compound Selenium Glutathione H2O2 Oxidized glutathione H2O
Type others amino acids,carboxyl group,peptide/protein,sulfhydryl group others amino acids,carboxyl group,peptide/protein,disulfide bond H2O
ChEBI 27568
 27568
 		16856
 16856
 		16240
 16240
 		17858
 17858
 		15377
 15377
PubChem 6326970
 6326970
 		124886
 25246407
 124886
 25246407
 		22326046
 784
 22326046
 784
 		11215652
 65359
 11215652
 65359
 		22247451
 962
 22247451
 962
1gp1A Bound:SEC Unbound Unbound Unbound
1gp1B Bound:SEC Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1gp1A SEC 45(Seleninic acid)
1gp1B SEC 45(Seleninic acid)

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[2]
p.66-68, Fig.13
[6]
Fig.1, p.304
[7]
p.216-218
[8]
p.90-91
[9]
p.147-149
[10]
p.878-880

References
[1]
Resource
Comments
Medline ID
PubMed ID 1140308
Journal Experientia
Year 1975
Volume 31
Pages 769-70
Authors Beutler E, Beutler B, Matsumoto J
Title Glutathione peroxidase activity of inorganic selenium and seleno-DL-cysteine.
Related PDB
Related UniProtKB
[2]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS)
Medline ID 83209650
PubMed ID 6852035
Journal Eur J Biochem
Year 1983
Volume 133
Pages 51-69
Authors Epp O, Ladenstein R, Wendel A
Title The refined structure of the selenoenzyme glutathione peroxidase at 0.2-nm resolution.
Related PDB 1gp1
Related UniProtKB P00435
[3]
Resource
Comments
Medline ID
PubMed ID 6714945
Journal Hoppe Seylers Z Physiol Chem
Year 1984
Volume 365
Pages 195-212
Authors Gunzler WA, Steffens GJ, Grossmann A, Kim SM, Otting F, Wendel A, Flohe L
Title The amino-acid sequence of bovine glutathione peroxidase.
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID 6233508
Journal Nutr Rev
Year 1984
Volume 42
Pages 168-9
Authors [No authors listed]
Title Position and function of the organic selenium in glutathione peroxidase.
Related PDB
Related UniProtKB
[5]
Resource
Comments
Medline ID
PubMed ID 2130181
Journal J Toxicol Clin Exp
Year 1990
Volume 10
Pages 375-83
Authors Bompart G
Title Cisplatin-induced changes on cytochrome P-450, lipid peroxidation and some P-450 related specific catalytic activities in rat liver.
Related PDB
Related UniProtKB
[6]
Resource
Comments
Medline ID
PubMed ID 7651210
Journal Methods Enzymol
Year 1995
Volume 251
Pages 303-14
Authors Biewenga GP, Bast A
Title Reaction of lipoic acid with ebselen and hypochlorous acid.
Related PDB
Related UniProtKB
[7]
Resource
Comments
Medline ID
PubMed ID 7766257
Journal Z Naturforsch [C]
Year 1995
Volume 50
Pages 209-19
Authors Prutz WA
Title Glutathione peroxidase-like activity of simple selenium compounds. Peroxides and the heterocyclic N-oxide resazurin acting as O-atom donors.
Related PDB
Related UniProtKB
[8]
Resource
Comments
Medline ID
PubMed ID 8912640
Journal Biochem Biophys Res Commun
Year 1996
Volume 228
Pages 88-93
Authors Hou Y, Guo Z, Li J, Wang PG
Title Seleno compounds and glutathione peroxidase catalyzed decomposition of S-nitrosothiols.
Related PDB
Related UniProtKB
[9]
Resource
Comments
Medline ID
PubMed ID 9315305
Journal Biomed Environ Sci
Year 1997
Volume 10
Pages 136-55
Authors Aumann KD, Bedorf N, Brigelius-Flohe R, Schomburg D, Flohe L
Title Glutathione peroxidase revisited--simulation of the catalytic cycle by computer-assisted molecular modelling.
Related PDB
Related UniProtKB
[10]
Resource
Comments
Medline ID
PubMed ID 9180378
Journal J Mol Biol
Year 1997
Volume 268
Pages 869-85
Authors Ren B, Huang W, Akesson B, Ladenstein R
Title The crystal structure of seleno-glutathione peroxidase from human plasma at 2.9 A resolution.
Related PDB
Related UniProtKB
[11]
Resource
Comments
Medline ID
PubMed ID 9517485
Journal Biol Trace Elem Res
Year 1998
Volume 61
Pages 127-36
Authors Howard SA, Hawkes WC
Title The relative effectiveness of human plasma glutathione peroxidase as a catalyst for the reduction of hydroperoxides by glutathione.
Related PDB
Related UniProtKB
[12]
Resource
Comments
Medline ID
PubMed ID 9828014
Journal Protein Sci
Year 1998
Volume 7
Pages 2465-8
Authors Schroder E, Ponting CP
Title Evidence that peroxiredoxins are novel members of the thioredoxin fold superfamily.
Related PDB
Related UniProtKB
[13]
Resource
Comments
Medline ID
PubMed ID 11018712
Journal Biochim Biophys Acta
Year 2000
Volume 1481
Pages 222-8
Authors Liu J, Luo G, Ren X, Mu Y, Bai Y, Shen J
Title A bis-cyclodextrin diselenide with glutathione peroxidase-like activity.
Related PDB
Related UniProtKB
[14]
Resource
Comments
Medline ID
PubMed ID 10995212
Journal Bioconjug Chem
Year 2000
Volume 11
Pages 682-7
Authors Ren X, Liu J, Luo G, Zhang Y, Luo Y, Yan G, Shen J
Title A novel selenocystine-beta-cyclodextrin conjugate that acts as a glutathione peroxidase mimic.
Related PDB
Related UniProtKB
[15]
Resource
Comments
Medline ID
PubMed ID 11697854
Journal Arch Biochem Biophys
Year 2001
Volume 395
Pages 177-84
Authors Su D, Ren X, You D, Li D, Mu Y, Yan G, Zhang Y, Luo Y, Xue Y, Shen J, Liu Z, Luo G
Title Generation of three selenium-containing catalytic antibodies with high catalytic efficiency using a novel hapten design method.
Related PDB
Related UniProtKB
[16]
Resource
Comments
Medline ID
PubMed ID 11485327
Journal Biochem Biophys Res Commun
Year 2001
Volume 286
Pages 189-94
Authors Su D, You D, Ren X, Luo G, Mu Y, Yan G, Xue Y, Shen J
Title Kinetics study of a selenium-containing ScFv catalytic antibody that mimics glutathione peroxidase.
Related PDB
Related UniProtKB
[17]
Resource
Comments
Medline ID
PubMed ID 11456617
Journal J Am Chem Soc
Year 2001
Volume 123
Pages 839-50
Authors Mugesh G, Panda A, Singh HB, Punekar NS, Butcher RJ
Title Glutathione peroxidase-like antioxidant activity of diaryl diselenides: a mechanistic study.
Related PDB
Related UniProtKB
[18]
Resource
Comments
Medline ID
PubMed ID 11678710
Journal Org Lett
Year 2001
Volume 3
Pages 3569-72
Authors Goto K, Nagahama M, Mizushima T, Shimada K, Kawashima T, Okazaki R
Title The first direct oxidative conversion of a selenol to a stable selenenic acid: experimental demonstration of three processes included in the catalytic cycle of glutathione peroxidase.
Related PDB
Related UniProtKB
[19]
Resource
Comments
Medline ID
PubMed ID 12371844
Journal J Am Chem Soc
Year 2002
Volume 124
Pages 12104-5
Authors Back TG, Moussa Z
Title Remarkable activity of a novel cyclic seleninate ester as a glutathione peroxidase mimetic and its facile in situ generation from allyl 3-hydroxypropyl selenide.
Related PDB
Related UniProtKB
[20]
Resource
Comments
Medline ID
PubMed ID 11996219
Journal J Biotechnol
Year 2002
Volume 82
Pages 411-24
Authors Scheller FW, Wollenberger U, Lei C, Jin W, Ge B, Lehmann C, Lisdat F, Fridman V
Title Bioelectrocatalysis by redox enzymes at modified electrodes.
Related PDB
Related UniProtKB

Comments

Created Updated
2004-07-09 2009-02-26