DB code: D00866

CATH domain 3.40.30.10 : Glutaredoxin Catalytic domain
3.-.-.- : Catalytic domain
E.C. 1.11.1.15
CSA
M-CSA
MACiE

CATH domain Related DB codes (homologues)
3.40.30.10 : Glutaredoxin S00916 S00279 M00184 D00869 D00870 D00278

Uniprot Enzyme Name
UniprotKB Protein name Synonyms RefSeq Pfam
Q9Y9L0 Probable peroxiredoxin
EC 1.11.1.15
NP_148509.1 (Protein)
NC_000854.2 (DNA/RNA sequence)
PF10417 (1-cysPrx_C)
PF00578 (AhpC-TSA)
[Graphical View]

KEGG enzyme name
Peroxiredoxin
Thioredoxin peroxidase
Tryparedoxin peroxidase
Alkyl hydroperoxide reductase C22
AhpC
TrxPx
TXNPx
Prx
PRDX

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
Q9Y9L0 TDXH_AERPE 2 R'-SH + ROOH = R'-S-S-R' + H(2)O + ROH.

KEGG Pathways
Map code Pathways E.C.
MAP00480 Glutathione metabolism

Compound table
Substrates Products Intermediates
KEGG-id C16736 C15498 C15496 C00001 C01335 I00144 I00145 I00142 I00143
E.C.
Compound R'-SH ROOH R-S-S-R H2O ROH Peptidyl-His-Cys-sulfenic acid (hydroxy-imidazolyl-sulfanyl-methane) Peptidyl-protonated-His-Cys-sulfenic acid (hydroxy-protonated-imidazolyl-sulfanyl-methane) Peptidyl-Cys-sulfenic acid Transient disulfide bond between peptidyl-Cys
Type sulfhydryl group others disulfide bond H2O carbohydrate
ChEBI 15377
 15377
PubChem 22247451
 962
 22247451
 962
1vgsA01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1vgsB01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1vgsC01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1vgsD01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1vgsE01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1vgsF01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1vgsG01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1vgsH01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1vgsI01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1vgsJ01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1x0rA01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1x0rB01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1x0rC01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1x0rD01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1x0rE01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1x0rF01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1x0rG01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1x0rH01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1x0rI01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1x0rJ01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2cv4A01 Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:OCS Unbound
2cv4B01 Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:OCS Unbound
2cv4C01 Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:OCS Unbound
2cv4D01 Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:OCS Unbound
2cv4E01 Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:OCS Unbound
2cv4F01 Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:OCS Unbound
2cv4G01 Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:OCS Unbound
2cv4H01 Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:OCS Unbound
2cv4I01 Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:OCS Unbound
2cv4J01 Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:OCS Unbound
2e2gA01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2e2gB01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2e2gC01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2e2gD01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2e2gE01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2e2gF01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2e2gG01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2e2gH01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2e2gI01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2e2gJ01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2e2mA01 Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:CSD Unbound
2e2mB01 Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:CSD Unbound
2e2mC01 Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:CSD Unbound
2e2mD01 Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:CSD Unbound
2e2mE01 Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:CSD Unbound
2e2mF01 Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:CSD Unbound
2e2mG01 Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:CSD Unbound
2e2mH01 Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:CSD Unbound
2e2mI01 Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:CSD Unbound
2e2mJ01 Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:CSD Unbound
2nvlA01 Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:OCS Unbound
2nvlB01 Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:OCS Unbound
2nvlC01 Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:OCS Unbound
2nvlD01 Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:OCS Unbound
2nvlE01 Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:OCS Unbound
2nvlF01 Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:OCS Unbound
2nvlG01 Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:OCS Unbound
2nvlH01 Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:OCS Unbound
2nvlI01 Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:OCS Unbound
2nvlJ01 Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:OCS Unbound
2zctA01 Unbound Unbound Unbound Unbound Intermediate-bound:HIS_42-CSO_52 Unbound Unbound Unbound
2zctB01 Unbound Unbound Unbound Unbound Unbound Intermediate-bound:HIS_42-CSO_52 Unbound Unbound
2zctC01 Unbound Unbound Unbound Unbound Unbound Intermediate-bound:HIS_42-CSO_52 Unbound Unbound
2zctD01 Unbound Unbound Unbound Unbound Unbound Intermediate-bound:HIS_42-CSO_52 Unbound Unbound
2zctE01 Unbound Unbound Unbound Unbound Intermediate-bound:HIS_42-CSO_52 Unbound Unbound Unbound
2zctF01 Unbound Unbound Unbound Unbound Unbound Intermediate-bound:HIS_42-CSO_52 Unbound Unbound
2zctG01 Unbound Unbound Unbound Unbound Intermediate-bound:HIS_42-CSO_52 Unbound Unbound Unbound
2zctH01 Unbound Unbound Unbound Unbound Unbound Intermediate-bound:HIS_42-CSO_52 Unbound Unbound
2zctI01 Unbound Unbound Unbound Unbound Intermediate-bound:HIS_42-CSO_52 Unbound Unbound Unbound
2zctJ01 Unbound Unbound Unbound Unbound Intermediate-bound:HIS_42-CSO_52 Unbound Unbound Unbound
3a2vA01 Unbound Bound:PER Unbound Unbound Unbound Unbound Unbound Unbound
3a2vB01 Unbound Bound:PER Unbound Unbound Unbound Unbound Unbound Unbound
3a2vC01 Unbound Bound:PER Unbound Unbound Unbound Unbound Unbound Unbound
3a2vD01 Unbound Bound:PER Unbound Unbound Unbound Unbound Unbound Unbound
3a2vE01 Unbound Bound:PER Unbound Unbound Unbound Unbound Unbound Unbound
3a2vF01 Unbound Bound:PER Unbound Unbound Unbound Unbound Unbound Unbound
3a2vG01 Unbound Bound:PER Unbound Unbound Unbound Unbound Unbound Unbound
3a2vH01 Unbound Bound:PER Unbound Unbound Unbound Unbound Unbound Unbound
3a2vI01 Unbound Bound:PER Unbound Unbound Unbound Unbound Unbound Unbound
3a2vJ01 Unbound Bound:PER Unbound Unbound Unbound Unbound Unbound Unbound
3a2wA01 Unbound Unbound Unbound Analogue:GOL Unbound Unbound Unbound Unbound
3a2wB01 Unbound Bound:PER Unbound Unbound Unbound Unbound Unbound Unbound
3a2wC01 Unbound Unbound Unbound Analogue:GOL Unbound Unbound Unbound Unbound
3a2wD01 Unbound Unbound Unbound Analogue:GOL Unbound Unbound Unbound Unbound
3a2wE01 Unbound Unbound Unbound Analogue:GOL Unbound Unbound Unbound Unbound
3a2wF01 Unbound Bound:PER Unbound Unbound Unbound Unbound Unbound Unbound
3a2wG01 Unbound Unbound Unbound Analogue:GOL Unbound Unbound Unbound Unbound
3a2wH01 Unbound Unbound Unbound Analogue:GOL Unbound Unbound Unbound Unbound
3a2wI01 Unbound Bound:PER Unbound Unbound Unbound Unbound Unbound Unbound
3a2wJ01 Unbound Unbound Unbound Analogue:GOL Unbound Unbound Unbound Unbound
3a2xA01 Unbound Analogue:ACT Unbound Unbound Unbound Unbound Unbound Unbound
3a2xB01 Unbound Analogue:ACT Unbound Unbound Unbound Unbound Unbound Unbound
3a2xC01 Unbound Analogue:ACT Unbound Unbound Unbound Unbound Unbound Unbound
3a2xD01 Unbound Analogue:ACT Unbound Unbound Unbound Unbound Unbound Unbound
3a2xE01 Unbound Analogue:ACT Unbound Unbound Unbound Unbound Unbound Unbound
3a2xF01 Unbound Analogue:ACT Unbound Unbound Unbound Unbound Unbound Unbound
3a2xG01 Unbound Analogue:ACT Unbound Unbound Unbound Unbound Unbound Unbound
3a2xH01 Unbound Analogue:ACT Unbound Unbound Unbound Unbound Unbound Unbound
3a2xI01 Unbound Analogue:ACT Unbound Unbound Unbound Unbound Unbound Unbound
3a2xJ01 Unbound Analogue:ACT Unbound Unbound Unbound Unbound Unbound Unbound
3a5wA01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a5wB01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a5wC01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a5wD01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a5wE01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a5wF01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a5wG01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a5wH01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a5wI01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a5wJ01 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1vgsA02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1vgsB02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1vgsC02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1vgsD02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1vgsE02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1vgsF02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1vgsG02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1vgsH02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1vgsI02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1vgsJ02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1x0rA02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1x0rB02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1x0rC02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1x0rD02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1x0rE02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1x0rF02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1x0rG02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1x0rH02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1x0rI02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1x0rJ02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2cv4A02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2cv4B02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2cv4C02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2cv4D02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2cv4E02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2cv4F02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2cv4G02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2cv4H02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2cv4I02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2cv4J02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2e2gA02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2e2gB02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2e2gC02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2e2gD02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2e2gE02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2e2gF02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2e2gG02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2e2gH02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2e2gI02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2e2gJ02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2e2mA02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2e2mB02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2e2mC02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2e2mD02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2e2mE02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2e2mF02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2e2mG02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2e2mH02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2e2mI02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2e2mJ02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2nvlA02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2nvlB02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2nvlC02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2nvlD02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2nvlE02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2nvlF02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2nvlG02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2nvlH02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2nvlI02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2nvlJ02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2zctA02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2zctB02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2zctC02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2zctD02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2zctE02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2zctF02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2zctG02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2zctH02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2zctI02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2zctJ02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a2vA02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a2vB02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a2vC02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a2vD02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a2vE02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a2vF02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a2vG02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a2vH02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a2vI02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a2vJ02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a2wA02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a2wB02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a2wC02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a2wD02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a2wE02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a2wF02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a2wG02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a2wH02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a2wI02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a2wJ02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a2xA02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a2xB02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a2xC02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a2xD02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a2xE02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a2xF02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a2xG02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a2xH02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a2xI02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a2xJ02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a5wA02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a5wB02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a5wC02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a5wD02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a5wE02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a5wF02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a5wG02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a5wH02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a5wI02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3a5wJ02 Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.
Swiss-prot;Q9Y9L0 & Literature [3]

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1vgsA01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
1vgsB01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
1vgsC01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
1vgsD01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
1vgsE01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
1vgsF01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
1vgsG01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
1vgsH01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
1vgsI01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
1vgsJ01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
1x0rA01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
1x0rB01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
1x0rC01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
1x0rD01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
1x0rE01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
1x0rF01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
1x0rG01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
1x0rH01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
1x0rI01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
1x0rJ01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
2cv4A01 HIS 42;THR 47; ;ARG 126 OCS 50 (Cys-sulfonic acid) VAL 49;OCS 50
2cv4B01 HIS 42;THR 47; ;ARG 126 OCS 50 (Cys-sulfonic acid) VAL 49;OCS 50
2cv4C01 HIS 42;THR 47; ;ARG 126 OCS 50 (Cys-sulfonic acid) VAL 49;OCS 50
2cv4D01 HIS 42;THR 47; ;ARG 126 OCS 50 (Cys-sulfonic acid) VAL 49;OCS 50
2cv4E01 HIS 42;THR 47; ;ARG 126 OCS 50 (Cys-sulfonic acid) VAL 49;OCS 50
2cv4F01 HIS 42;THR 47; ;ARG 126 OCS 50 (Cys-sulfonic acid) VAL 49;OCS 50
2cv4G01 HIS 42;THR 47; ;ARG 126 OCS 50 (Cys-sulfonic acid) VAL 49;OCS 50
2cv4H01 HIS 42;THR 47; ;ARG 126 OCS 50 (Cys-sulfonic acid) VAL 49;OCS 50
2cv4I01 HIS 42;THR 47; ;ARG 126 OCS 50 (Cys-sulfonic acid) VAL 49;OCS 50
2cv4J01 HIS 42;THR 47; ;ARG 126 OCS 50 (Cys-sulfonic acid) VAL 49;OCS 50
2e2gA01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
2e2gB01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
2e2gC01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
2e2gD01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
2e2gE01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
2e2gF01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
2e2gG01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
2e2gH01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
2e2gI01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
2e2gJ01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
2e2mA01 HIS 42;THR 47; ;ARG 126 CSD 50 (Cys-sulfinic acid) VAL 49;CSD 50
2e2mB01 HIS 42;THR 47; ;ARG 126 CSD 50 (Cys-sulfinic acid) VAL 49;CSD 50
2e2mC01 HIS 42;THR 47; ;ARG 126 CSD 50 (Cys-sulfinic acid) VAL 49;CSD 50
2e2mD01 HIS 42;THR 47; ;ARG 126 CSD 50 (Cys-sulfinic acid) VAL 49;CSD 50
2e2mE01 HIS 42;THR 47; ;ARG 126 CSD 50 (Cys-sulfinic acid) VAL 49;CSD 50
2e2mF01 HIS 42;THR 47; ;ARG 126 CSD 50 (Cys-sulfinic acid) VAL 49;CSD 50
2e2mG01 HIS 42;THR 47; ;ARG 126 CSD 50 (Cys-sulfinic acid) VAL 49;CSD 50
2e2mH01 HIS 42;THR 47; ;ARG 126 CSD 50 (Cys-sulfinic acid) VAL 49;CSD 50
2e2mI01 HIS 42;THR 47; ;ARG 126 CSD 50 (Cys-sulfinic acid) VAL 49;CSD 50
2e2mJ01 HIS 42;THR 47; ;ARG 126 CSD 50 (Cys-sulfinic acid) VAL 49;CSD 50
2nvlA01 HIS 42;THR 47; ;ARG 126 OCS 50 (Cys-sulfonic acid) VAL 49;OCS 50
2nvlB01 HIS 42;THR 47; ;ARG 126 OCS 50 (Cys-sulfonic acid) VAL 49;OCS 50
2nvlC01 HIS 42;THR 47; ;ARG 126 OCS 50 (Cys-sulfonic acid) VAL 49;OCS 50
2nvlD01 HIS 42;THR 47; ;ARG 126 OCS 50 (Cys-sulfonic acid) VAL 49;OCS 50
2nvlE01 HIS 42;THR 47; ;ARG 126 OCS 50 (Cys-sulfonic acid) VAL 49;OCS 50
2nvlF01 HIS 42;THR 47; ;ARG 126 OCS 50 (Cys-sulfonic acid) VAL 49;OCS 50
2nvlG01 HIS 42;THR 47; ;ARG 126 OCS 50 (Cys-sulfonic acid) VAL 49;OCS 50
2nvlH01 HIS 42;THR 47; ;ARG 126 OCS 50 (Cys-sulfonic acid) VAL 49;OCS 50
2nvlI01 HIS 42;THR 47; ;ARG 126 OCS 50 (Cys-sulfonic acid) VAL 49;OCS 50
2nvlJ01 HIS 42;THR 47; ;ARG 126 OCS 50 (Cys-sulfonic acid) VAL 49;OCS 50
2zctA01 HIS 42;THR 47; ;ARG 126 CSO 50 (Cys-sulfenic acid) VAL 49;CSO 50
2zctB01 HIS 42;THR 47; ;ARG 126 CSO 50 (Cys-sulfenic acid) VAL 49;CSO 50
2zctC01 HIS 42;THR 47; ;ARG 126 CSO 50 (Cys-sulfenic acid) VAL 49;CSO 50
2zctD01 HIS 42;THR 47; ;ARG 126 CSO 50 (Cys-sulfenic acid) VAL 49;CSO 50
2zctE01 HIS 42;THR 47; ;ARG 126 CSO 50 (Cys-sulfenic acid) VAL 49;CSO 50
2zctF01 HIS 42;THR 47; ;ARG 126 CSO 50 (Cys-sulfenic acid) VAL 49;CSO 50
2zctG01 HIS 42;THR 47; ;ARG 126 CSO 50 (Cys-sulfenic acid) VAL 49;CSO 50
2zctH01 HIS 42;THR 47; ;ARG 126 CSO 50 (Cys-sulfenic acid) VAL 49;CSO 50
2zctI01 HIS 42;THR 47; ;ARG 126 CSO 50 (Cys-sulfenic acid) VAL 49;CSO 50
2zctJ01 HIS 42;THR 47; ;ARG 126 CSO 50 (Cys-sulfenic acid) VAL 49;CSO 50
3a2vA01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
3a2vB01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
3a2vC01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
3a2vD01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
3a2vE01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
3a2vF01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
3a2vG01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
3a2vH01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
3a2vI01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
3a2vJ01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
3a2wA01 HIS 42;THR 47; ;ARG 126 VAL 49; mutant C50S
3a2wB01 HIS 42;THR 47; ;ARG 126 VAL 49; mutant C50S
3a2wC01 HIS 42;THR 47; ;ARG 126 VAL 49; mutant C50S
3a2wD01 HIS 42;THR 47; ;ARG 126 VAL 49; mutant C50S
3a2wE01 HIS 42;THR 47; ;ARG 126 VAL 49; mutant C50S
3a2wF01 HIS 42;THR 47; ;ARG 126 VAL 49; mutant C50S
3a2wG01 HIS 42;THR 47; ;ARG 126 VAL 49; mutant C50S
3a2wH01 HIS 42;THR 47; ;ARG 126 VAL 49; mutant C50S
3a2wI01 HIS 42;THR 47; ;ARG 126 VAL 49; mutant C50S
3a2wJ01 HIS 42;THR 47; ;ARG 126 VAL 49; mutant C50S
3a2xA01 HIS 42;THR 47; ;ARG 126 VAL 49; mutant C50S
3a2xB01 HIS 42;THR 47; ;ARG 126 VAL 49; mutant C50S
3a2xC01 HIS 42;THR 47; ;ARG 126 VAL 49; mutant C50S
3a2xD01 HIS 42;THR 47; ;ARG 126 VAL 49; mutant C50S
3a2xE01 HIS 42;THR 47; ;ARG 126 VAL 49; mutant C50S
3a2xF01 HIS 42;THR 47; ;ARG 126 VAL 49; mutant C50S
3a2xG01 HIS 42;THR 47; ;ARG 126 VAL 49; mutant C50S
3a2xH01 HIS 42;THR 47; ;ARG 126 VAL 49; mutant C50S
3a2xI01 HIS 42;THR 47; ;ARG 126 VAL 49; mutant C50S
3a2xJ01 HIS 42;THR 47; ;ARG 126 VAL 49; mutant C50S
3a5wA01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
3a5wB01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
3a5wC01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
3a5wD01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
3a5wE01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
3a5wF01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
3a5wG01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
3a5wH01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
3a5wI01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
3a5wJ01 HIS 42;THR 47;CYS 50;ARG 126 VAL 49;CYS 50
1vgsA02 CYS 207;CYS 213
1vgsB02 CYS 207;CYS 213
1vgsC02 CYS 207;CYS 213
1vgsD02 CYS 207;CYS 213
1vgsE02 CYS 207;CYS 213
1vgsF02 CYS 207;CYS 213
1vgsG02 CYS 207;CYS 213
1vgsH02 CYS 207;CYS 213
1vgsI02 CYS 207;CYS 213
1vgsJ02 CYS 207;CYS 213
1x0rA02 ;CYS 213 mutant C207S
1x0rB02 ;CYS 213 mutant C207S
1x0rC02 ;CYS 213 mutant C207S
1x0rD02 ;CYS 213 mutant C207S
1x0rE02 ;CYS 213 mutant C207S
1x0rF02 ;CYS 213 mutant C207S
1x0rG02 ;CYS 213 mutant C207S
1x0rH02 ;CYS 213 mutant C207S
1x0rI02 ;CYS 213 mutant C207S
1x0rJ02 ;CYS 213 mutant C207S
2cv4A02 CYS 207;CYS 213
2cv4B02 CYS 207;CYS 213
2cv4C02 CYS 207;CYS 213
2cv4D02 CYS 207;CYS 213
2cv4E02 CYS 207;CYS 213
2cv4F02 CYS 207;CYS 213
2cv4G02 CYS 207;CYS 213
2cv4H02 CYS 207;CYS 213
2cv4I02 CYS 207;CYS 213
2cv4J02 CYS 207;CYS 213
2e2gA02 ;CYS 213 mutant C207S
2e2gB02 ;CYS 213 mutant C207S
2e2gC02 ;CYS 213 mutant C207S
2e2gD02 ;CYS 213 mutant C207S
2e2gE02 ;CYS 213 mutant C207S
2e2gF02 ;CYS 213 mutant C207S
2e2gG02 ;CYS 213 mutant C207S
2e2gH02 ;CYS 213 mutant C207S
2e2gI02 ;CYS 213 mutant C207S
2e2gJ02 ;CYS 213 mutant C207S
2e2mA02 ;CYS 213 mutant C207S
2e2mB02 ;CYS 213 mutant C207S
2e2mC02 ;CYS 213 mutant C207S
2e2mD02 ;CYS 213 mutant C207S
2e2mE02 ;CYS 213 mutant C207S
2e2mF02 ;CYS 213 mutant C207S
2e2mG02 ;CYS 213 mutant C207S
2e2mH02 ;CYS 213 mutant C207S
2e2mI02 ;CYS 213 mutant C207S
2e2mJ02 ;CYS 213 mutant C207S
2nvlA02 ;CYS 213 mutant C207S
2nvlB02 ;CYS 213 mutant C207S
2nvlC02 ;CYS 213 mutant C207S
2nvlD02 ;CYS 213 mutant C207S
2nvlE02 ;CYS 213 mutant C207S
2nvlF02 ;CYS 213 mutant C207S
2nvlG02 ;CYS 213 mutant C207S
2nvlH02 ;CYS 213 mutant C207S
2nvlI02 ;CYS 213 mutant C207S
2nvlJ02 ;CYS 213 mutant C207S
2zctA02 ;CYS 213 mutant C207S
2zctB02 ;CYS 213 mutant C207S
2zctC02 ;CYS 213 mutant C207S
2zctD02 ;CYS 213 mutant C207S
2zctE02 ;CYS 213 mutant C207S
2zctF02 ;CYS 213 mutant C207S
2zctG02 ;CYS 213 mutant C207S
2zctH02 ;CYS 213 mutant C207S
2zctI02 ;CYS 213 mutant C207S
2zctJ02 ;CYS 213 mutant C207S
3a2vA02 ;CYS 213 mutant C207S
3a2vB02 ;CYS 213 mutant C207S
3a2vC02 ;CYS 213 mutant C207S
3a2vD02 ;CYS 213 mutant C207S
3a2vE02 ;CYS 213 mutant C207S
3a2vF02 ;CYS 213 mutant C207S
3a2vG02 ;CYS 213 mutant C207S
3a2vH02 ;CYS 213 mutant C207S
3a2vI02 ;CYS 213 mutant C207S
3a2vJ02 ;CYS 213 mutant C207S
3a2wA02 CYS 207;CYS 213
3a2wB02 CYS 207;CYS 213
3a2wC02 CYS 207;CYS 213
3a2wD02 CYS 207;CYS 213
3a2wE02 CYS 207;CYS 213
3a2wF02 CYS 207;CYS 213
3a2wG02 CYS 207;CYS 213
3a2wH02 CYS 207;CYS 213
3a2wI02 CYS 207;CYS 213
3a2wJ02 CYS 207;CYS 213
3a2xA02 CYS 207;CYS 213
3a2xB02 CYS 207;CYS 213
3a2xC02 CYS 207;CYS 213
3a2xD02 CYS 207;CYS 213
3a2xE02 CYS 207;CYS 213
3a2xF02 CYS 207;CYS 213
3a2xG02 CYS 207;CYS 213
3a2xH02 CYS 207;CYS 213
3a2xI02 CYS 207;CYS 213
3a2xJ02 CYS 207;CYS 213
3a5wA02 CYS 207;CYS 213
3a5wB02 CYS 207;CYS 213
3a5wC02 CYS 207;CYS 213
3a5wD02 CYS 207;CYS 213
3a5wE02 CYS 207;CYS 213
3a5wF02 CYS 207;CYS 213
3a5wG02 CYS 207;CYS 213
3a5wH02 CYS 207;CYS 213
3a5wI02 CYS 207;CYS 213
3a5wJ02 CYS 207;CYS 213

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[3]
Figure 9, p.324-326
[5]
Fig. 4
[6]
Fig. 4

References
[1]
Resource
Comments
Medline ID
PubMed ID 12707274
Journal J Biol Chem
Year 2003
Volume 278
Pages 24174-80
Authors Jeon SJ, Ishikawa K
Title Characterization of novel hexadecameric thioredoxin peroxidase from Aeropyrum pernix K1.
Related PDB
Related UniProtKB
[2]
Resource
Comments
Medline ID
PubMed ID 12517450
Journal Trends Biochem Sci
Year 2003
Volume 28
Pages 32-40
Authors Wood ZA, Schroder E, Robin Harris J, Poole LB
Title Structure, mechanism and regulation of peroxiredoxins.
Related PDB
Related UniProtKB
[3]
Resource
Comments
Medline ID
PubMed ID 16214169
Journal J Mol Biol
Year 2005
Volume 354
Pages 317-29
Authors Mizohata E, Sakai H, Fusatomi E, Terada T, Murayama K, Shirouzu M, Yokoyama S
Title Crystal structure of an archaeal peroxiredoxin from the aerobic hyperthermophilic crenarchaeon Aeropyrum pernix K1.
Related PDB 1vgs 2cv4
Related UniProtKB Q9Y9L0
[4]
Resource
Comments
Medline ID
PubMed ID 16342268
Journal Proteins
Year 2006
Volume 62
Pages 822-6
Authors Nakamura T, Yamamoto T, Inoue T, Matsumura H, Kobayashi A, Hagihara Y, Uegaki K, Ataka M, Kai Y, Ishikawa K
Title Crystal structure of thioredoxin peroxidase from aerobic hyperthermophilic archaeon Aeropyrum pernix K1.
Related PDB 1x0r
Related UniProtKB Q9Y9L0
[5]
Resource
Comments
Medline ID
PubMed ID 18436649
Journal Proc Natl Acad Sci U S A
Year 2008
Volume 105
Pages 6238-42
Authors Nakamura T, Yamamoto T, Abe M, Matsumura H, Hagihara Y, Goto T, Yamaguchi T, Inoue T
Title Oxidation of archaeal peroxiredoxin involves a hypervalent sulfur intermediate.
Related PDB 2e2g 2e2m 2nvl 2zct
Related UniProtKB Q9Y9L0
[6]
Resource
Comments
Medline ID
PubMed ID 19819903
Journal J Biochem
Year 2010
Volume 147
Pages 109-15
Authors Nakamura T, Kado Y, Yamaguchi T, Matsumura H, Ishikawa K, Inoue T
Title Crystal structure of peroxiredoxin from Aeropyrum pernix K1 complexed with its substrate, hydrogen peroxide.
Related PDB 3a2v 3a2w 3a2x 3a5w
Related UniProtKB Q9Y9L0

Comments
Generally, peroxiredoxins (Prxs) can be classified into three categories (see [2]):
(1) typical 2-Cys Prxs; conservation of two redox-active cysteines; homodimers having two identical active sites.
(2) atypical 2-Cys Prxs; conservation of two redox-active cysteines; functionally monomeric.
(3) 1-Cys Prxs; only one cysteine.
The redox-active cysteine is referred to as the peroxidatic cysteine, in contrast to the second cysteine, the resolving cysteine, in the 2-Cys Prxs.
However, although this archaeal enzyme shares high sequence similarities with 1-Cys Prxs, it has three cysteine residues (see [1]). Cys50 is the peroxidatic cysteine, whereas Cys213 seems to be the resolving cysteine (see [3]). Although the functional role of Cys207 is unknown, it may form disulfide bond with Cys213, under oxidative stress (see [3]). Under such a stress, Cys50 can be over-oxidized to form sulfinic acid (-SO2H) and further oxidized sulfonic acid (-SO3H) (see [3]).
Although this enzyme is homologous to other Prxs, it has a histidine residue, which is not conserved, at the active site (see [5], [6]). Due to the histidine, this enzyme seems to have a distinct reaction mechanism, where hypervalent sulfur intermediates (I00144, I00145) (see [5], [6]).
Thus, this enzyme catalyzes the following reactions (see [3], [5], [6]). Here, the first two reactions, (A) and (B), seem to occur simultaneously to form a hypervalent sulfur intermediate (His-Cys-sulfenic acid; I00144) (see [5], [6]), which is a very rare case.
(A) Transfer of peroxide oxygen from another peroxide oxygen to the perdoxidatic Cys (Cys50):
(B) Addition of sulfur atom of the peroxidatic Cys to the imidazole ring of the histidine residue (His42):
The reaction mechanisms of the next two reactions, (C) and (D), have not been elucidated yet. At least, a protonation to the hypervalent sulfur intermediate (I00144) leads to another intermediate (I00145) (see [5]).
(C) Elimination of sulfur atom of the peroxidatic Cys from the histidine residue:
(D) Transfer of sulfur atom of the peroxidatic Cys (Cys50) from the hydroxyl group to thiol (or sulfhydryl) group of the resolving Cys (Cys213), releasing H2O and forming a disulfide bond:
(E) Electron transfer from thiol of another substrate (or R'-SH; thioredoxin) to the disulfide bond, forming a intermolecular disulfide bond (thiol-disulfide exchange):
(F) Electron transfer from another thiol of the second substrate (R'-SH; thioredoxin) to the disulfide bond, forming a intramolecular disulfide bond (thiol-disulfide exchange):

Created Updated
2012-06-25 2012-09-25