DB code: D00517
| RLCP classification | 3.770.220000.47 : Transfer | |
|---|---|---|
| CATH domain | 3.30.160.70 : Double Stranded RNA Binding Domain | |
| 1.10.10.10 : Arc Repressor Mutant, subunit A | Catalytic domain | |
| E.C. | 2.1.1.63 | |
| CSA | ||
| M-CSA | ||
| MACiE | ||
| CATH domain | Related DB codes (homologues) |
|---|---|
| 1.10.10.10 : Arc Repressor Mutant, subunit A | D00510 D00452 D00077 T00055 T00113 |
| Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | RefSeq | Pfam |
|---|---|---|---|---|
| O74023 |
Methylated-DNA--protein-cysteine methyltransferase
|
EC
2.1.1.63
6-O-methylguanine-DNA methyltransferase MGMT O-6-methylguanine-DNA-alkyltransferase Pk-MGMT |
YP_184384.1
(Protein)
NC_006624.1 (DNA/RNA sequence) |
PF01035
(DNA_binding_1)
PF09153 (DUF1938) [Graphical View] |
| Q58924 |
Methylated-DNA--protein-cysteine methyltransferase
|
EC
2.1.1.63
6-O-methylguanine-DNA methyltransferase MGMT O-6-methylguanine-DNA-alkyltransferase |
NP_248537.1
(Protein)
NC_000909.1 (DNA/RNA sequence) |
PF01035
(DNA_binding_1)
[Graphical View] |
| Q973C7 |
Methylated-DNA--protein-cysteine methyltransferase
|
EC
2.1.1.63
6-O-methylguanine-DNA methyltransferase MGMT O-6-methylguanine-DNA-alkyltransferase |
NP_376877.1
(Protein)
NC_003106.2 (DNA/RNA sequence) |
PF01035
(DNA_binding_1)
[Graphical View] |
| KEGG enzyme name |
|---|
|
methylated-DNA---[protein]-cysteine S-methyltransferase
|
| UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
|---|---|---|---|---|---|
| O74023 | OGT_PYRKO | DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L- cysteine. | Cytoplasm. | ||
| Q58924 | OGT_METJA | DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L- cysteine. | Cytoplasm (By similarity). | ||
| Q973C7 | OGT_SULTO | DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L- cysteine. | Cytoplasm (By similarity). |
| KEGG Pathways | Map code | Pathways | E.C. |
|---|
| Compound table | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Substrates | Products | Intermediates | ||||||||
| KEGG-id | C04250 | C02743 | C00039 | C03800 | ||||||
| E.C. | ||||||||||
| Compound | DNA (containing 6-O-methylguanine) | Protein L-cysteine | DNA | Protein S-methyl-L-cysteine | ||||||
| Type | amine group,carbohydrate,nucleic acids | peptide/protein,sulfhydryl group | nucleic acids | peptide/protein,sulfide group | ||||||
| ChEBI | ||||||||||
| PubChem | ||||||||||
| 1mgtA01 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | |
| 2g7hA01 |
|
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|
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|
Unbound | Unbound | Unbound | Unbound | |
| 1wrjA01 |
|
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|
|
|
Unbound | Unbound | Unbound | Unbound | |
| 1mgtA02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | |
| 2g7hA02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | |
| 1wrjA02 |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | |
| Reference for Active-site residues | ||
|---|---|---|
| resource | references | E.C. |
| see D00077 | ||
| Active-site residues | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| PDB | Catalytic residues | Cofactor-binding residues | Modified residues | Main-chain involved in catalysis | Comment | |||||
| 1mgtA01 |
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| 2g7hA01 |
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| 1wrjA01 |
|
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|||||
| 1mgtA02 |
|
|
|
|
|
TYR 112;ASN 133;CYS 141;HIS 142;GLU 167 | ||||
| 2g7hA02 |
|
|
|
|
|
TYR 99;ASN 120;CYS 128;HIS 129;GLU 154 | ||||
| 1wrjA02 |
|
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|
|
|
TYR 91;ASN 112;CYS 120;HIS 121;GLU 146 | ||||
| References for Catalytic Mechanism | ||
|---|---|---|
| References | Sections | No. of steps in catalysis |
|
[4]
|
p.713 | |
|
[7]
|
Fig.5, p.159-160 | 7 |
| References | |
|---|---|
| [1] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1581542 |
| Journal | Chem Res Toxicol |
| Year | 1992 |
| Volume | 5 |
| Pages | 8-9 |
| Authors | Kohda K, Terashima I, Sawada N, Nozaki I, Yasuda M, Kawazoe Y |
| Title | Synthesis and O-demethylation rate of cis-[Pt(NH3)2(O6,9-dimethylguanine-7)2]Cl2 |
| Related PDB | |
| Related UniProtKB | |
| [2] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1554415 |
| Journal | Mol Carcinog |
| Year | 1992 |
| Volume | 5 |
| Pages | 161-9 |
| Authors | Santibanez-Koref M, Elder RH, Fan CY, Cawkwell L, McKie JH, Douglas KT, Margison GP, Rafferty JA |
| Title | Isolation and partial characterization of murine O6-alkylguanine-DNA-alkyltransferase: comparative sequence and structural properties. |
| Related PDB | |
| Related UniProtKB | |
| [3] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7727387 |
| Journal | Biochemistry |
| Year | 1994 |
| Volume | 33 |
| Pages | 11364-71 |
| Authors | Spratt TE, Campbell CR |
| Title | Synthesis of oligodeoxynucleotides containing analogs of O6-methylguanine and reaction with O6-alkylguanine-DNA alkyltransferase |
| Related PDB | |
| Related UniProtKB | |
| [4] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 10497033 |
| Journal | J Mol Biol |
| Year | 1999 |
| Volume | 292 |
| Pages | 707-16 |
| Authors | Hashimoto H, Inoue T, Nishioka M, Fujiwara S, Takagi M, Imanaka T, Kai Y |
| Title | Hyperthermostable protein structure maintained by intra and inter-helix ion-pairs in archaeal O6-methylguanine-DNA methyltransferase. |
| Related PDB | 1mgt |
| Related UniProtKB | |
| [5] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10749683 |
| Journal | Biochem J |
| Year | 2000 |
| Volume | 347 |
| Pages | 527-34 |
| Authors | Xu-Welliver M, Kanugula S, Loktionova NA, Crone TM, Pegg AE |
| Title | Conserved residue lysine165 is essential for the ability of O6-alkylguanine-DNA alkyltransferase to react with O6-benzylguanine. |
| Related PDB | |
| Related UniProtKB | |
| [6] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10749682 |
| Journal | Biochem J |
| Year | 2000 |
| Volume | 347 |
| Pages | 519-26 |
| Authors | Xu-Welliver M, Pegg AE |
| Title |
Point mutations at multiple sites including highly conserved amino acids maintain activity, |
| Related PDB | |
| Related UniProtKB | |
| [7] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10946226 |
| Journal | Mutat Res |
| Year | 2000 |
| Volume | 460 |
| Pages | 151-63 |
| Authors | Daniels DS, Tainer JA |
| Title | Conserved structural motifs governing the stoichiometric repair of alkylated DNA by O(6)-alkylguanine-DNA alkyltransferase. |
| Related PDB | |
| Related UniProtKB | |
| [8] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11488906 |
| Journal | Eur J Biochem |
| Year | 2001 |
| Volume | 268 |
| Pages | 4144-50 |
| Authors | Shiraki K, Nishikori S, Fujiwara S, Hashimoto H, Kai Y, Takagi M, Imanaka T |
| Title | Comparative analyses of the conformational stability of a hyperthermophilic protein and its mesophilic counterpart. |
| Related PDB | |
| Related UniProtKB | |
| [9] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11398466 |
| Journal | Methods Enzymol |
| Year | 2001 |
| Volume | 334 |
| Pages | 239-48 |
| Authors | Takagi M, Kai Y, Imanaka T |
| Title | Methylguanine methyltransferase from Thermococcus kodakaraensis KOD1 |
| Related PDB | |
| Related UniProtKB | |
| [10] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12093287 |
| Journal | Biochemistry |
| Year | 2002 |
| Volume | 41 |
| Pages | 8689-97 |
| Authors | Luu KX, Kanugula S, Pegg AE, Pauly GT, Moschel RC |
| Title | Repair of oligodeoxyribonucleotides by O(6)-alkylguanine-DNA alkyltransferase |
| Related PDB | |
| Related UniProtKB | |
| [11] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 16826543 |
| Journal | Magn Reson Chem |
| Year | 2006 |
| Volume | 44 Spec No |
| Pages | S71-82 |
| Authors | Roberts A, Pelton JG, Wemmer DE |
| Title |
Structural studies of MJ1529, |
| Related PDB | 2g7h |
| Related UniProtKB | |
| Comments |
|---|
|
The catalytic domain of this enzyme (from Archaea) is homologous to that of its homologue from human (D00077 in EzCatDB). |
| Created | Updated |
|---|---|
| 2003-04-25 | 2009-02-26 |