DB code: D00452
| CATH domain | 1.10.10.10 : Arc Repressor Mutant, subunit A | Catalytic domain |
|---|---|---|
| 3.40.1360.10 : Dna Topoisomerase Vi A Subunit; Chain | ||
| E.C. | 5.99.1.3 | |
| CSA | ||
| M-CSA | ||
| MACiE | ||
| CATH domain | Related DB codes (homologues) |
|---|---|
| 1.10.10.10 : Arc Repressor Mutant, subunit A | D00510 D00077 D00517 T00055 T00113 |
| Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | RefSeq | Pfam |
|---|---|---|---|---|
| Q57815 |
Type II DNA topoisomerase VI subunit A
|
EC
5.99.1.3
|
NP_247343.1
(Protein)
NC_000909.1 (DNA/RNA sequence) |
PF04406
(TP6A_N)
[Graphical View] |
| KEGG enzyme name |
|---|
|
DNA topoisomerase (ATP-hydrolysing)
type II DNA topoisomerase DNA-gyrase deoxyribonucleate topoisomerase deoxyribonucleic topoisomerase topoisomerase DNA topoisomerase II |
| UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
|---|---|---|---|---|---|
| Q57815 | TOP6A_METJA | ATP-dependent breakage, passage and rejoining of double-stranded DNA. | Heterotetramer of two subunits A and two subunits B. | Binds 1 magnesium ion per subunit. |
| KEGG Pathways | Map code | Pathways | E.C. |
|---|
| Compound table | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cofactors | Substrates | Products | Intermediates | |||||||||
| KEGG-id | C00305 | C00434 | C00002 | C00434 | C00008 | C00009 | ||||||
| E.C. | ||||||||||||
| Compound | Magnesium | Double-stranded DNA | ATP | Double-stranded DNA | ADP | Orthophosphate | ||||||
| Type | divalent metal (Ca2+, Mg2+) | nucleic acids | amine group,nucleotide | nucleic acids | amine group,nucleotide | phosphate group/phosphate ion | ||||||
| ChEBI |
18420 18420 |
15422 15422 |
16761 16761 |
26078 26078 |
||||||||
| PubChem |
888 888 |
5957 5957 |
6022 6022 |
1004 22486802 1004 22486802 |
||||||||
| 1d3yA01 |
|
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|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1d3yB01 |
|
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|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1d3yA02 |
|
|
|
|
|
Bound:_MG | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1d3yB02 |
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|
Bound:_MG | Unbound | Unbound | Unbound | Unbound | Unbound | |
| Reference for Active-site residues | ||
|---|---|---|
| resource | references | E.C. |
| Swiss-prot;Q57815 | ||
| Active-site residues | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| PDB | Catalytic residues | Cofactor-binding residues | Modified residues | Main-chain involved in catalysis | Comment | |||||
| 1d3yA01 |
|
|
|
|
|
TYR 103 | ||||
| 1d3yB01 |
|
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|
|
TYR 103 | ||||
| 1d3yA02 |
|
|
|
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|
GLU 197;ASP 249(Magnesium binding) | ||||
| 1d3yB02 |
|
|
|
|
|
GLU 197;ASP 249(Magnesium binding) | ||||
| References for Catalytic Mechanism | ||
|---|---|---|
| References | Sections | No. of steps in catalysis |
| References | |
|---|---|
| [1] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9519293 |
| Journal | Curr Opin Struct Biol |
| Year | 1998 |
| Volume | 8 |
| Pages | 26-32 |
| Authors | Berger JM |
| Title | Type II DNA topoisomerases. |
| Related PDB | |
| Related UniProtKB | |
| [2] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9801313 |
| Journal | Nucleic Acids Res |
| Year | 1998 |
| Volume | 26 |
| Pages | 5157-62 |
| Authors | Buhler C, Gadelle D, Forterre P, Wang JC, Bergerat A |
| Title | Reconstitution of DNA topoisomerase VI of the thermophilic archaeon Sulfolobus shibatae from subunits separately overexpressed in Escherichia coli. |
| Related PDB | |
| Related UniProtKB | |
| [3] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 10545127 |
| Journal | EMBO J |
| Year | 1999 |
| Volume | 18 |
| Pages | 6177-88 |
| Authors | Nichols MD, DeAngelis K, Keck JL, Berger JM |
| Title | Structure and function of an archaeal topoisomerase VI subunit with homology to the meiotic recombination factor Spo11. |
| Related PDB | 1d3y |
| Related UniProtKB | |
| [4] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11809802 |
| Journal | Mol Cell Biol |
| Year | 2002 |
| Volume | 22 |
| Pages | 1106-15 |
| Authors | Diaz RL, Alcid AD, Berger JM, Keeney S |
| Title | Identification of residues in yeast Spo11p critical for meiotic DNA double-strand break formation. |
| Related PDB | |
| Related UniProtKB | |
| [5] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12596227 |
| Journal | Bioessays |
| Year | 2003 |
| Volume | 25 |
| Pages | 232-42 |
| Authors | Gadelle D, Filee J, Buhler C, Forterre P |
| Title | Phylogenomics of type II DNA topoisomerases. |
| Related PDB | |
| Related UniProtKB | |
| [6] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12505993 |
| Journal | EMBO J |
| Year | 2003 |
| Volume | 22 |
| Pages | 151-63 |
| Authors | Corbett KD, Berger JM |
| Title | Structure of the topoisomerase VI-B subunit: implications for type II topoisomerase mechanism and evolution. |
| Related PDB | |
| Related UniProtKB | |
| Comments |
|---|
|
Topoisomerase Type VI is an archaeal topoisomerase type II.
|
| Created | Updated |
|---|---|
| 2004-04-22 | 2009-02-26 |