DB code: D00170
| CATH domain | 1.10.530.10 : Lysozyme | Catalytic domain |
|---|---|---|
| 3.30.20.10 : Endochitinase; domain 2 | Catalytic domain | |
| E.C. | 3.2.1.14 | |
| CSA | 1cns | |
| M-CSA | 1cns | |
| MACiE | ||
| CATH domain | Related DB codes (homologues) |
|---|---|
| 1.10.530.10 : Lysozyme | S00520 S00509 |
| Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | CAZy | Pfam |
|---|---|---|---|---|
| P23951 |
26 kDa endochitinase 2
|
EC
3.2.1.14
CHI-26 |
GH19
(Glycoside Hydrolase Family 19)
|
PF00182
(Glyco_hydro_19)
[Graphical View] |
| O81934 |
|
Chitinase
|
GH19
(Glycoside Hydrolase Family 19)
|
PF00182
(Glyco_hydro_19)
[Graphical View] |
| KEGG enzyme name |
|---|
|
chitinase
chitodextrinase 1,4-beta-poly-N-acetylglucosaminidase poly-beta-glucosaminidase beta-1,4-poly-N-acetyl glucosamidinase poly[1,4-(N-acetyl-beta-D-glucosaminide)] glycanohydrolase |
| UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
|---|---|---|---|---|---|
| P23951 | CHI2_HORVU | Random hydrolysis of N-acetyl-beta-D- glucosaminide (1->4)-beta-linkages in chitin and chitodextrins. | |||
| O81934 | O81934_CANEN |
| KEGG Pathways | Map code | Pathways | E.C. |
|---|---|---|
| MAP00530 | Aminosugars metabolism |
| Compound table | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Substrates | Products | Intermediates | |||||||||
| KEGG-id | C00461 | C00851 | C00001 | C03518 | C00140 | ||||||
| E.C. | |||||||||||
| Compound | Chitin | Chitodextrin | H2O | N-Acetyl-D-glucosaminide | N-Acetyl-D-glucosamine | ||||||
| Type | amide group,polysaccharide | amide group,polysaccharide | H2O | amide group,carbohydrate | amide group,carbohydrate | ||||||
| ChEBI |
15377 15377 |
506227 506227 |
|||||||||
| PubChem |
22247451 962 22247451 962 |
439174 439174 |
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| 1baaA01 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1cnsA01 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1cnsB01 |
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Unbound | Unbound | Unbound | Unbound | ||
| 2baaA01 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1dxjA01 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1baaA02 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1cnsA02 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1cnsB02 |
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Unbound | Unbound | Unbound | Unbound | ||
| 2baaA02 |
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Unbound | Unbound | Unbound | Unbound | ||
| 1dxjA02 |
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Unbound | Unbound | Unbound | Unbound | ||
| Reference for Active-site residues | ||
|---|---|---|
| resource | references | E.C. |
| Active-site residues | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| PDB | Catalytic residues | Cofactor-binding residues | Modified residues | Main-chain involved in catalysis | Comment | |||||
| 1baaA01 |
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|
GLU 67 | ||||
| 1cnsA01 |
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|
GLU 67 | ||||
| 1cnsB01 |
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GLU 67 | ||||
| 2baaA01 |
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|
GLU 67 | ||||
| 1dxjA01 |
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|
GLU 68 | ||||
| 1baaA02 |
|
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|
GLU 89 | ||||
| 1cnsA02 |
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|
GLU 89 | ||||
| 1cnsB02 |
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|
GLU 89 | ||||
| 2baaA02 |
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|
GLU 89 | ||||
| 1dxjA02 |
|
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|
GLU 90 | ||||
| References for Catalytic Mechanism | ||
|---|---|---|
| References | Sections | No. of steps in catalysis |
|
[3]
|
p.410-411 | |
| References | |
|---|---|
| [1] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1593638 |
| Journal | J Mol Biol |
| Year | 1992 |
| Volume | 225 |
| Pages | 565-7 |
| Authors | Hart PJ, Ready MP, Robertus JD |
| Title |
Crystallization of an endochitinase from Hordeum vulgar L. |
| Related PDB | |
| Related UniProtKB | |
| [2] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) |
| Medline ID | 93132784 |
| PubMed ID | 8421299 |
| Journal | J Mol Biol |
| Year | 1993 |
| Volume | 229 |
| Pages | 189-93 |
| Authors | Hart PJ, Monzingo AF, Ready MP, Ernst SR, Robertus JD |
| Title |
Crystal structure of an endochitinase from Hordeum vulgare L. |
| Related PDB | |
| Related UniProtKB | P23951 |
| [3] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 7739049 |
| Journal | J Mol Biol |
| Year | 1995 |
| Volume | 248 |
| Pages | 402-13 |
| Authors | Hart PJ, Pfluger HD, Monzingo AF, Hollis T, Robertus JD |
| Title |
The refined crystal structure of an endochitinase from Hordeum vulgare L. |
| Related PDB | 1baa 2baa |
| Related UniProtKB | |
| [4] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | |
| Journal | Acta Crystallogr D Biol Crystallogr |
| Year | 1996 |
| Volume | 52 |
| Pages | 289-98 |
| Authors | Song HK, Suh SW |
| Title | Refined structure of the chitinase from barley seeds at 2.0 angstrom resolution. |
| Related PDB | 1cns |
| Related UniProtKB | |
| [5] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8831791 |
| Journal | J Mol Biol |
| Year | 1996 |
| Volume | 262 |
| Pages | 243-57 |
| Authors | Terwisscha van Scheltinga AC, Hennig M, Dijkstra BW |
| Title |
The 1.8 A resolution structure of hevamine, |
| Related PDB | |
| Related UniProtKB | |
| [6] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9148754 |
| Journal | Biochem J |
| Year | 1997 |
| Volume | 322 |
| Pages | 815-22 |
| Authors | Andersen MD, Jensen A, Robertus JD, Leah R, Skriver K |
| Title | Heterologous expression and characterization of wild-type and mutant forms of a 26 kDa endochitinase from barley (Hordeum vulgare L.). |
| Related PDB | |
| Related UniProtKB | |
| [7] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9790582 |
| Journal | Mol Gen Genet |
| Year | 1998 |
| Volume | 259 |
| Pages | 511-5 |
| Authors | Ohme-Takagi M, Meins F Jr, Shinshi H |
| Title | A tobacco gene encoding a novel basic class II chitinase: a putative ancestor of basic class I and acidic class II chitinase genes. |
| Related PDB | |
| Related UniProtKB | |
| [8] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | |
| Journal | Acta Crystallogr D Biol Crystallogr |
| Year | 2000 |
| Volume | 56 |
| Pages | 1096-9 |
| Authors | Hahn M, Hennig M, Schlesier B, Hohne W |
| Title | Structure of jack bean chitinase. |
| Related PDB | 1dxj |
| Related UniProtKB | |
| [9] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11414619 |
| Journal | Plant Mol Biol |
| Year | 2001 |
| Volume | 45 |
| Pages | 609-18 |
| Authors | Suarez V, Staehelin C, Arango R, Holtorf H, Hofsteenge J, Meins F Jr |
| Title | Substrate specificity and antifungal activity of recombinant tobacco class I chitinases. |
| Related PDB | |
| Related UniProtKB | |
| Comments |
|---|
| Created | Updated |
|---|---|
| 2004-03-25 | 2009-09-29 |