DB code: T00232

CATH domain 3.40.50.10490 : Rossmann fold Catalytic domain
3.40.50.10490 : Rossmann fold Catalytic domain
1.10.1390.10 : Phosphoglucose isomerase, C-terminal domain Catalytic domain
E.C. 5.3.1.9
CSA 1dqr
M-CSA 1dqr
MACiE

CATH domain Related DB codes (homologues)
3.40.50.10490 : Rossmann fold T00201

Uniprot Enzyme Name
UniprotKB Protein name Synonyms Pfam RefSeq
P13376 Glucose-6-phosphate isomerase B
GPI B
EC 5.3.1.9
Phosphoglucose isomerase B
PGI-B
Phosphohexose isomerase B
PHI-B
PF00342 (PGI)
[Graphical View]
Q9N1E2 Glucose-6-phosphate isomerase
GPI
EC 5.3.1.9
Phosphoglucose isomerase
PGI
Phosphohexose isomerase
PHI
PF00342 (PGI)
[Graphical View] 		NP_001075538.1 (Protein)
NM_001082069.2 (DNA/RNA sequence)
P08059 Glucose-6-phosphate isomerase
GPI
EC 5.3.1.9
Phosphoglucose isomerase
PGI
Phosphohexose isomerase
PHI
PF00342 (PGI)
[Graphical View] 		NP_999495.1 (Protein)
NM_214330.1 (DNA/RNA sequence)
P06744 Glucose-6-phosphate isomerase
GPI
EC 5.3.1.9
Phosphoglucose isomerase
PGI
Phosphohexose isomerase
PHI
Neuroleukin
NLK
Sperm antigen 36
SA-36
PF00342 (PGI)
[Graphical View] 		NP_000166.2 (Protein)
NM_000175.3 (DNA/RNA sequence)
NP_001171651.1 (Protein)
NM_001184722.1 (DNA/RNA sequence)

KEGG enzyme name
glucose-6-phosphate isomerase
phosphohexose isomerase
phosphohexomutase
oxoisomerase
hexosephosphate isomerase
phosphosaccharomutase
phosphoglucoisomerase
phosphohexoisomerase
phosphoglucose isomerase
glucose phosphate isomerase
hexose phosphate isomerase
D-glucose-6-phosphate ketol-isomerase

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P13376 G6PIB_BACST D-glucose 6-phosphate = D-fructose 6- phosphate. Homodimer. Cytoplasm.
Q9N1E2 G6PI_RABIT D-glucose 6-phosphate = D-fructose 6- phosphate. Homodimer. Cytoplasm.
P08059 G6PI_PIG D-glucose 6-phosphate = D-fructose 6- phosphate. Homodimer. Cytoplasm.
P06744 G6PI_HUMAN D-glucose 6-phosphate = D-fructose 6- phosphate. Homodimer. Cytoplasm.

KEGG Pathways
Map code Pathways E.C.
MAP00010 Glycolysis / Gluconeogenesis
MAP00030 Pentose phosphate pathway
MAP00500 Starch and sucrose metabolism

Compound table
Substrates Products Intermediates
KEGG-id C00092 C00085
E.C.
Compound D-Glucose 6-phosphate D-Fructose 6-phosphate Intermediate-1 Intermediate-2
Type carbohydrate,phosphate group/phosphate ion carbohydrate,phosphate group/phosphate ion
ChEBI 4170
 4170
 		61553
 61553
PubChem 5958
 5958
 		439160
 439160
1b0zA01 Unbound Unbound Unbound Unbound
1c7qA01 Unbound Unbound Unbound Unbound
1c7rA01 Unbound Unbound Unbound Intermediate-analogue:PA5
2pgiA01 Unbound Unbound Unbound Unbound
1dqrA01 Unbound Unbound Intermediate-analogue:6PG Unbound
1dqrB01 Unbound Unbound Intermediate-analogue:6PG Unbound
1g98A01 Unbound Unbound Unbound Intermediate-analogue:PA5
1g98B01 Unbound Unbound Unbound Intermediate-analogue:PA5
1hm5A01 Unbound Unbound Unbound Unbound
1hm5B01 Unbound Unbound Unbound Unbound
1hoxA01 Unbound Bound:F6P Unbound Unbound
1hoxB01 Unbound Bound:F6P Unbound Unbound
1kojA01 Unbound Unbound Unbound Intermediate-analogue:PAN
1kojB01 Unbound Unbound Unbound Intermediate-analogue:PAN
1n8tA01 Unbound Unbound Unbound Unbound
1n8tB01 Unbound Unbound Unbound Unbound
1gzdA01 Unbound Unbound Unbound Unbound
1gzvA01 Unbound Unbound Unbound Intermediate-analogue:PA5
1iatA01 Unbound Unbound Unbound Unbound
1iriA01 Unbound Unbound Unbound Intermediate-analogue:E4P
1iriB01 Unbound Unbound Unbound Intermediate-analogue:E4P
1iriC01 Unbound Unbound Unbound Intermediate-analogue:E4P
1iriD01 Unbound Unbound Unbound Intermediate-analogue:E4P
1jiqA01 Unbound Unbound Unbound Unbound
1jiqB01 Unbound Unbound Unbound Unbound
1jiqC01 Unbound Unbound Unbound Unbound
1jiqD01 Unbound Unbound Unbound Unbound
1jlhA01 Unbound Unbound Unbound Unbound
1jlhB01 Unbound Unbound Unbound Unbound
1jlhC01 Unbound Unbound Unbound Unbound
1jlhD01 Unbound Unbound Unbound Unbound
1nuhA01 Unbound Unbound Unbound Intermediate-analogue:PA5
1b0zA02 Unbound Unbound Unbound Unbound
1c7qA02 Unbound Unbound Unbound Unbound
1c7rA02 Unbound Unbound Unbound Unbound
2pgiA02 Unbound Unbound Unbound Unbound
1dqrA02 Unbound Unbound Unbound Unbound
1dqrB02 Unbound Unbound Unbound Unbound
1g98A02 Unbound Unbound Unbound Unbound
1g98B02 Unbound Unbound Unbound Unbound
1hm5A02 Unbound Unbound Unbound Unbound
1hm5B02 Unbound Unbound Unbound Unbound
1hoxA02 Unbound Unbound Unbound Unbound
1hoxB02 Unbound Unbound Unbound Unbound
1kojA02 Unbound Unbound Unbound Unbound
1kojB02 Unbound Unbound Unbound Unbound
1n8tA02 Unbound Unbound Unbound Unbound
1n8tB02 Unbound Unbound Unbound Unbound
1gzdA02 Unbound Unbound Unbound Unbound
1gzvA02 Unbound Unbound Unbound Unbound
1iatA02 Unbound Unbound Unbound Unbound
1iriA02 Unbound Unbound Unbound Unbound
1iriB02 Unbound Unbound Unbound Unbound
1iriC02 Unbound Unbound Unbound Unbound
1iriD02 Unbound Unbound Unbound Unbound
1jiqA02 Unbound Unbound Unbound Unbound
1jiqB02 Unbound Unbound Unbound Unbound
1jiqC02 Unbound Unbound Unbound Unbound
1jiqD02 Unbound Unbound Unbound Unbound
1jlhA02 Unbound Unbound Unbound Unbound
1jlhB02 Unbound Unbound Unbound Unbound
1jlhC02 Unbound Unbound Unbound Unbound
1jlhD02 Unbound Unbound Unbound Unbound
1nuhA02 Unbound Unbound Unbound Unbound
1b0zA03 Unbound Unbound Unbound Unbound
1c7qA03 Unbound Unbound Unbound Unbound
1c7rA03 Unbound Unbound Unbound Unbound
2pgiA03 Unbound Unbound Unbound Unbound
1dqrA03 Unbound Unbound Unbound Unbound
1dqrB03 Unbound Unbound Unbound Unbound
1g98A03 Unbound Unbound Unbound Unbound
1g98B03 Unbound Unbound Unbound Unbound
1hm5A03 Unbound Unbound Unbound Unbound
1hm5B03 Unbound Unbound Unbound Unbound
1hoxA03 Unbound Unbound Unbound Unbound
1hoxB03 Unbound Unbound Unbound Unbound
1kojA03 Unbound Unbound Unbound Unbound
1kojB03 Unbound Unbound Unbound Unbound
1n8tA03 Unbound Unbound Unbound Unbound
1n8tB03 Unbound Unbound Unbound Unbound
1gzdA03 Unbound Unbound Unbound Unbound
1gzvA03 Unbound Unbound Unbound Unbound
1iatA03 Unbound Unbound Unbound Unbound
1iriA03 Unbound Unbound Unbound Unbound
1iriB03 Unbound Unbound Unbound Unbound
1iriC03 Unbound Unbound Unbound Unbound
1iriD03 Unbound Unbound Unbound Unbound
1jiqA03 Unbound Unbound Unbound Unbound
1jiqB03 Unbound Unbound Unbound Unbound
1jiqC03 Unbound Unbound Unbound Unbound
1jiqD03 Unbound Unbound Unbound Unbound
1jlhA03 Unbound Unbound Unbound Unbound
1jlhB03 Unbound Unbound Unbound Unbound
1jlhC03 Unbound Unbound Unbound Unbound
1jlhD03 Unbound Unbound Unbound Unbound
1nuhA03 Unbound Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.
Swiss-prot;P13376 & literature [13]

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1b0zA01 GLU 285;HIS 306
1c7qA01 GLU 285;HIS 306
1c7rA01 GLU 285;HIS 306
2pgiA01 GLU 285;HIS 306
1dqrA01 GLU 357;HIS 388
1dqrB01 GLU 357;HIS 388
1g98A01 GLU 357;HIS 388
1g98B01 GLU 357;HIS 388
1hm5A01 GLU 357;HIS 388
1hm5B01 GLU 357;HIS 388
1hoxA01 GLU 357;HIS 388
1hoxB01 GLU 357;HIS 388
1kojA01 GLU 357;HIS 388
1kojB01 GLU 357;HIS 388
1n8tA01 GLU 357;HIS 388
1n8tB01 GLU 357;HIS 388
1gzdA01 GLU 357;HIS 388
1gzvA01 GLU 357;HIS 388
1iatA01 GLU 357;HIS 388
1iriA01 GLU 358;HIS 389
1iriB01 GLU 358;HIS 389
1iriC01 GLU 358;HIS 389
1iriD01 GLU 358;HIS 389
1jiqA01 GLU 358;HIS 389
1jiqB01 GLU 358;HIS 389
1jiqC01 GLU 358;HIS 389
1jiqD01 GLU 358;HIS 389
1jlhA01 GLU 357;HIS 388
1jlhB01 GLU 357;HIS 388
1jlhC01 GLU 357;HIS 388
1jlhD01 GLU 357;HIS 388
1nuhA01 GLU 357;HIS 388
1b0zA02 GLU 145
1c7qA02 GLU 145
1c7rA02 GLU 145
2pgiA02 GLU 145
1dqrA02 GLU 216
1dqrB02 GLU 216
1g98A02 GLU 216
1g98B02 GLU 216
1hm5A02 GLU 216
1hm5B02 GLU 216
1hoxA02 GLU 216
1hoxB02 GLU 216
1kojA02 GLU 216
1kojB02 GLU 216
1n8tA02 GLU 216
1n8tB02 GLU 216
1gzdA02 GLU 216
1gzvA02 GLU 216
1iatA02 GLU 216
1iriA02 GLU 217
1iriB02 GLU 217
1iriC02 GLU 217
1iriD02 GLU 217
1jiqA02 GLU 217
1jiqB02 GLU 217
1jiqC02 GLU 217
1jiqD02 GLU 217
1jlhA02 GLU 216
1jlhB02 GLU 216
1jlhC02 GLU 216
1jlhD02 GLU 216
1nuhA02 GLU 216
1b0zA03 LYS 420
1c7qA03 LYS 420
1c7rA03 LYS 420
2pgiA03 LYS 420
1dqrA03 LYS 518
1dqrB03 LYS 518
1g98A03 LYS 518
1g98B03 LYS 518
1hm5A03 LYS 518
1hm5B03 LYS 518
1hoxA03 LYS 518
1hoxB03 LYS 518
1kojA03 LYS 518
1kojB03 LYS 518
1n8tA03 LYS 518
1n8tB03 LYS 518
1gzdA03 LYS 518
1gzvA03 LYS 518
1iatA03 LYS 518
1iriA03 LYS 519
1iriB03 LYS 519
1iriC03 LYS 519
1iriD03 LYS 519
1jiqA03 LYS 519
1jiqB03 LYS 519
1jiqC03 LYS 519
1jiqD03 LYS 519
1jlhA03 LYS 518
1jlhB03 LYS 518
1jlhC03 LYS 518
1jlhD03 LYS 518
1nuhA03 LYS 518

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[5]
Fig.1a, p.146- 2
[8]
Scheme I, Scheme II
[11]
Fig.1, p.2442 2
[12]
Fig.3, p.958-961 4
[13]
Fig.6, p.23158-23160 5
[15]
Fig.4, p.1564-1566 4
[16]
Fig.3B, Fig.4, p.7802-7804 2
[17]
Fig.3, p.12-14 2
[18]
Fig.8, p.455-458 4
[23]
Fig.7, p.462-463 4
[25]
p.121-122

References
[1]
Resource
Comments
Medline ID
PubMed ID
Journal J Mol Biol
Year 1977
Volume 109
Pages 475-85
Authors Shaw PJ, Muirhead H
Title Crystallographic Structure Analysis of Glucose-6-Phosphate Isomerase at 3.5 A Resolution.
Related PDB 1b0z
Related UniProtKB
[2]
Resource
Comments
Medline ID
PubMed ID 7410429
Journal J Biol Chem
Year 1980
Volume 255
Pages 9369-74
Authors Gibson DR, Gracy RW, Hartman FC
Title Affinity labeling and characterization of the active site histidine of glucosephosphate isomerase. Sequence homology with triosephosphate isomerase.
Related PDB
Related UniProtKB
[3]
Resource
Comments
Medline ID
PubMed ID 6798937
Journal Arch Biochem Biophys
Year 1981
Volume 212
Pages 347-59
Authors Lu HS, Gracy RW
Title Specific cleavage of glucosephosphate isomerases at cysteinyl residues using 2-nitro-5-thiocyanobenzoic acid: analyses of peptides eluted from polyacrylamide gels and localization of active site histidyl and lysyl residues.
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID 7225356
Journal Biochemistry
Year 1981
Volume 20
Pages 1756-61
Authors Schnackerz KD, Chirgwin JM, Noltmann EA
Title Synthesis of 1-chloro-2-oxohexanol 6-phosphate, a covalent active-site reagent for phosphoglucose isomerase.
Related PDB
Related UniProtKB
[5]
Resource
Comments
Medline ID
PubMed ID 6115414
Journal Philos Trans R Soc Lond B Biol Sci
Year 1981
Volume 293
Pages 145-57
Authors Achari A, Marshall SE, Muirhead H, Palmieri RH, Noltmann EA
Title Glucose-6-phosphate isomerase.
Related PDB
Related UniProtKB
[6]
Resource
Comments
Medline ID
PubMed ID 6762366
Journal Isozymes Curr Top Biol Med Res
Year 1982
Volume 6
Pages 169-205
Authors Gracy RW
Title Glucosephosphate and triosephosphate isomerases: significance of isozyme structural differences in evolution, physiology, and aging.
Related PDB
Related UniProtKB
[7]
Resource
Comments
Medline ID
PubMed ID 6838203
Journal Arch Biochem Biophys
Year 1983
Volume 221
Pages 489-98
Authors Pullan LM, Igarashi P, Noltmann EA
Title Arginine-specific modification of rabbit muscle phosphoglucose isomerase: differences in the inactivation by phenylglyoxal and butanedione and in the protection by substrate analogs.
Related PDB
Related UniProtKB
[8]
Resource
Comments
Medline ID
PubMed ID 8381960
Journal Proc Natl Acad Sci U S A
Year 1993
Volume 90
Pages 1237-41
Authors Seeholzer SH
Title Phosphoglucose isomerase: a ketol isomerase with aldol C2-epimerase activity.
Related PDB
Related UniProtKB
[9]
Resource
Comments
Medline ID
PubMed ID 9417984
Journal J Struct Biol
Year 1997
Volume 120
Pages 196-200
Authors Hsiao CD, Chou CC, Hsiao YY, Sun YJ, Meng M
Title Expression, purification, and crystallization of two isozymes of 6-phosphoglucose isomerase of Bacillus stearothermophilus.
Related PDB
Related UniProtKB
[10]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
Medline ID 99254054
PubMed ID 10318897
Journal Proc Natl Acad Sci U S A
Year 1999
Volume 96
Pages 5412-7
Authors Sun YJ, Chou CC, Chen WS, Wu RT, Meng M, Hsiao CD
Title The crystal structure of a multifunctional protein: phosphoglucose isomerase/autocrine motility factor/neuroleukin.
Related PDB 2pgi
Related UniProtKB P13376
[11]
Resource
Comments
Medline ID
PubMed ID 10595547
Journal Protein Sci
Year 1999
Volume 8
Pages 2438-43
Authors Meng M, Chane TL, Sun YJ, Hsiao CD
Title Probing the location and function of the conserved histidine residue of phosphoglucose isomerase by using an active site directed inhibitor N-bromoacetylethanolamine phosphate.
Related PDB
Related UniProtKB
[12]
Resource
Comments
Medline ID
PubMed ID 10653639
Journal Biochemistry
Year 2000
Volume 39
Pages 955-64
Authors Jeffery CJ, Bahnson BJ, Chien W, Ringe D, Petsko GA
Title Crystal structure of rabbit phosphoglucose isomerase, a glycolytic enzyme that moonlights as neuroleukin, autocrine motility factor, and differentiation mediator.
Related PDB 1dqr
Related UniProtKB
[13]
Resource
Comments
Medline ID
PubMed ID 10770936
Journal J Biol Chem
Year 2000
Volume 275
Pages 23154-60
Authors Chou CC, Sun YJ, Meng M, Hsiao CD
Title The crystal structure of phosphoglucose isomerase/autocrine motility factor/neuroleukin complexed with its carbohydrate phosphate inhibitors suggests its substrate/receptor recognition.
Related PDB 1c7q 1c7r
Related UniProtKB
[14]
Resource
Comments
Medline ID
PubMed ID 11030090
Journal J Enzyme Inhib
Year 2000
Volume 15
Pages 509-15
Authors Hardre R, Salmon L, Opperdoes FR
Title Competitive inhibition of Trypanosoma brucei phosphoglucose isomerase by D-arabinose-5-phosphate derivatives.
Related PDB
Related UniProtKB
[15]
Resource
Comments
Medline ID
PubMed ID 11327814
Journal Biochemistry
Year 2001
Volume 40
Pages 1560-6
Authors Jeffery CJ, Hardre R, Salmon L
Title Crystal structure of rabbit phosphoglucose isomerase complexed with 5-phospho-D-arabinonate identifies the role of Glu357 in catalysis.
Related PDB 1g98
Related UniProtKB
[16]
Resource
Comments
Medline ID
PubMed ID 11425306
Journal Biochemistry
Year 2001
Volume 40
Pages 7799-805
Authors Lee JH, Chang KZ, Patel V, Jeffery CJ
Title Crystal structure of rabbit phosphoglucose isomerase complexed with its substrate D-fructose 6-phosphate.
Related PDB 1hox
Related UniProtKB
[17]
Resource
Comments
Medline ID
PubMed ID 11418102
Journal FEBS Lett
Year 2001
Volume 499
Pages 11-4
Authors Meng M, Lin HY, Hsieh CJ, Chen YT
Title Functions of the conserved anionic amino acids and those interacting with the substrate phosphate group of phosphoglucose isomerase.
Related PDB
Related UniProtKB
[18]
Resource
Comments
Medline ID
PubMed ID 11371164
Journal J Mol Biol
Year 2001
Volume 309
Pages 447-63
Authors Read J, Pearce J, Li X, Muirhead H, Chirgwin J, Davies C
Title The crystal structure of human phosphoglucose isomerase at 1.6 A resolution: implications for catalytic mechanism, cytokine activity and haemolytic anaemia.
Related PDB 1iat
Related UniProtKB
[19]
Resource
Comments
Medline ID
PubMed ID 12368100
Journal J Mol Biol
Year 2002
Volume 323
Pages 77-84
Authors Arsenieva D, Jeffery CJ
Title Conformational changes in phosphoglucose isomerase induced by ligand binding.
Related PDB 1hm5
Related UniProtKB
[20]
Resource
Comments
Medline ID
PubMed ID 11983887
Journal Proc Natl Acad Sci U S A
Year 2002
Volume 99
Pages 5872-7
Authors Arsenieva D, Hardre R, Salmon L, Jeffery CJ
Title The crystal structure of rabbit phosphoglucose isomerase complexed with 5-phospho-D-arabinonohydroxamic acid.
Related PDB 1koj
Related UniProtKB
[21]
Resource
Comments
Medline ID
PubMed ID 12402366
Journal Proteins
Year 2002
Volume 49
Pages 577-9
Authors Davies C, Muirhead H
Title Crystal structure of phosphoglucose isomerase from pig muscle and its complex with 5-phosphoarabinonate.
Related PDB 1gzd 1gzv
Related UniProtKB
[22]
Resource
Comments
Medline ID
PubMed ID 12054796
Journal J Mol Biol
Year 2002
Volume 318
Pages 985-97
Authors Tanaka N, Haga A, Uemura H, Akiyama H, Funasaka T, Nagase H, Raz A, Nakamura KT
Title Inhibition mechanism of cytokine activity of human autocrine motility factor examined by crystal structure analyses and site-directed mutagenesis studies.
Related PDB 1iri 1jiq
Related UniProtKB
[23]
Resource
Comments
Medline ID
PubMed ID 12595702
Journal Acta Crystallogr D Biol Crystallogr
Year 2003
Volume 59
Pages 453-65
Authors Davies C, Muirhead H
Title Structure of native phosphoglucose isomerase from rabbit: conformational changes associated with catalytic function.
Related PDB 1n8t
Related UniProtKB
[24]
Resource
Comments
Medline ID
PubMed ID 12777791
Journal Acta Crystallogr D Biol Crystallogr
Year 2003
Volume 59
Pages 1111-3
Authors Davies C, Muirhead H, Chirgwin J
Title The structure of human phosphoglucose isomerase complexed with a transition-state analogue.
Related PDB 1nuh
Related UniProtKB
[25]
Resource
Comments
Medline ID
PubMed ID 12573240
Journal Biochim Biophys Acta
Year 2003
Volume 1645
Pages 117-22
Authors Cordeiro AT, Godoi PH, Silva CH, Garratt RC, Oliva G, Thiemann OH
Title Crystal structure of human phosphoglucose isomerase and analysis of the initial catalytic steps.
Related PDB 1jlh
Related UniProtKB

Comments

Created Updated
2004-05-20 2009-02-26