DB code: S00381

CATH domain 3.40.50.7700 : Rossmann fold Catalytic domain
E.C. 4.1.1.21
CSA
M-CSA
MACiE

CATH domain Related DB codes (homologues)

Uniprot Enzyme Name
UniprotKB Protein name Synonyms RefSeq Pfam
P0AG18 N5-carboxyaminoimidazole ribonucleotide mutase
N5-CAIR mutase
EC 5.4.99.18
5-(carboxyamino)imidazole ribonucleotide mutase
NP_415056.1 (Protein)
NC_000913.2 (DNA/RNA sequence)
YP_488812.1 (Protein)
NC_007779.1 (DNA/RNA sequence)
PF00731 (AIRC)
[Graphical View]

KEGG enzyme name
phosphoribosylaminoimidazole carboxylase
5-phosphoribosyl-5-aminoimidazole carboxylase
5-amino-1-ribosylimidazole 5-phosphate carboxylase
AIR carboxylase
1-(5-phosphoribosyl)-5-amino-4-imidazolecarboxylate carboxy-lyase
ADE2
class II PurE
5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate carboxy-lyase

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P0AG18 PUR6_ECOLI 5-amino-1-(5-phospho-D-ribosyl)imidazole-4- carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO(2). Homooctamer.

KEGG Pathways
Map code Pathways E.C.
MAP00230 Purine metabolism

Compound table
Substrates Products Intermediates
KEGG-id C04751 C00011 C03373
E.C.
Compound 1-(5-Phospho-D-ribosyl)-5-amino-4-imidazolecarboxylate CO2 1-(5-Phospho-D-ribosyl)-5-aminoimidazole
Type amine group,carboxyl group,nucleotide others amine group,nucleotide
ChEBI 28413
 28413
 		16526
 16526
PubChem 165388
 165388
 		280
 280
 		161500
 161500
1d7aA Unbound Unbound Bound:AIR
1d7aB Unbound Unbound Bound:AIR
1d7aC Unbound Unbound Bound:AIR
1d7aD Unbound Unbound Bound:AIR
1d7aL Unbound Unbound Unbound
1d7aM Unbound Unbound Unbound
1d7aN Unbound Unbound Unbound
1d7aO Unbound Unbound Unbound
1qczA Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1d7aA
1d7aB
1d7aC
1d7aD
1d7aL
1d7aM
1d7aN
1d7aO
1qczA

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[7]
Fig.4 3
[8]
p.1399-1402

References
[1]
Resource
Comments
Medline ID
PubMed ID 1797375
Journal Carbohydr Res
Year 1991
Volume 216
Pages 109-18
Authors Ewing DF, Holy A, Votruba I, Humble RW, Mackenzie G, Hewedi F, Shaw G
Title Synthesis of 4- and 5-amino-1-(2-deoxy-D-erythro-pentofuranosyl)imidazole nucleosides by chemical and biotransformation methods.
Related PDB
Related UniProtKB
[2]
Resource
Comments CHARACTERIZATION, AND SEQUENCE OF 1-10.
Medline ID 92287929
PubMed ID 1534690
Journal Biochemistry
Year 1992
Volume 31
Pages 5022-32
Authors Meyer E, Leonard NJ, Bhat B, Stubbe J, Smith JM
Title Purification and characterization of the purE, purK, and purC gene products: identification of a previously unrecognized energy requirement in the purine biosynthetic pathway.
Related PDB
Related UniProtKB P0AG18
[3]
Resource
Comments
Medline ID
PubMed ID 7918411
Journal Biochemistry
Year 1994
Volume 33
Pages 11927-34
Authors Firestine SM, Poon SW, Mueller EJ, Stubbe J, Davisson VJ
Title Reactions catalyzed by 5-aminoimidazole ribonucleotide carboxylases from Escherichia coli and Gallus gallus: a case for divergent catalytic mechanisms.
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID 8117684
Journal Biochemistry
Year 1994
Volume 33
Pages 2269-78
Authors Mueller EJ, Meyer E, Rudolph J, Davisson VJ, Stubbe J
Title N5-carboxyaminoimidazole ribonucleotide: evidence for a new intermediate and two new enzymatic activities in the de novo purine biosynthetic pathway of Escherichia coli.
Related PDB
Related UniProtKB
[5]
Resource
Comments
Medline ID
PubMed ID 9341908
Journal J Med Chem
Year 1997
Volume 40
Pages 3336-45
Authors Groziak MP, Huan ZW, Ding H, Meng Z, Stevens WC, Robinson PD
Title Effect of a chemical modification on the hydrated adenosine intermediate produced by adenosine deaminase and a model reaction for a potential mechanism of action of 5-aminoimidazole ribonucleotide carboxylase.
Related PDB
Related UniProtKB
[6]
Resource
Comments
Medline ID
PubMed ID 9500840
Journal Arch Biochem Biophys
Year 1998
Volume 351
Pages 123-34
Authors Firestine SM, Misialek S, Toffaletti DL, Klem TJ, Perfect JR, Davisson VJ
Title Biochemical role of the Cryptococcus neoformans ADE2 protein in fungal de novo purine biosynthesis.
Related PDB
Related UniProtKB
[7]
Resource
Comments
Medline ID
PubMed ID 10074353
Journal Biochemistry
Year 1999
Volume 38
Pages 3012-8
Authors Meyer E, Kappock TJ, Osuji C, Stubbe J
Title Evidence for the direct transfer of the carboxylate of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to generate 4-carboxy-5-aminoimidazole ribonucleotide catalyzed by Escherichia coli PurE, an N5-CAIR mutase.
Related PDB
Related UniProtKB
[8]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
Medline ID 20045189
PubMed ID 10574791
Journal Structure Fold Des
Year 1999
Volume 7
Pages 1395-406
Authors Mathews II, Kappock TJ, Stubbe J, Ealick SE
Title Crystal structure of Escherichia coli PurE, an unusual mutase in the purine biosynthetic pathway.
Related PDB 1d7a 1qcz
Related UniProtKB P0AG18

Comments

Created Updated
2004-03-31 2009-02-26